Patrice Francois

Summary

Affiliation: Genomic Research Laboratory
Country: Switzerland

Publications

  1. pmc Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureus
    Thibaud Koessler
    Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1040-8. 2006
  2. pmc Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
    Caroline Le Marechal
    INRA, UMR1253 Science et Technologie du Lait et de l Œuf, F 35042 Rennes, France
    Vet Res 42:35. 2011
  3. pmc Genomic analysis of an emerging multiresistant Staphylococcus aureus strain rapidly spreading in cystic fibrosis patients revealed the presence of an antibiotic inducible bacteriophage
    Jean Marc Rolain
    URMITE CNRS IRD UMR 6236, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biol Direct 4:1. 2009
  4. pmc Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation
    Qiang Lou
    Key Laboratory of Medical Molecular Virology of Ministry of Education and Ministry of Public Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, 138 Yixueyuan Road, Shanghai, 200032, PR China
    BMC Microbiol 11:146. 2011
  5. pmc Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus
    Kati Seidl
    Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland
    BMC Microbiol 9:95. 2009
  6. pmc Transcriptome analysis of the responses of Staphylococcus aureus to antimicrobial peptides and characterization of the roles of vraDE and vraSR in antimicrobial resistance
    Milla Pietiäinen
    Antimicrobial Resistance Unit, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare THL, PL 30, 00271 Helsinki, Finland
    BMC Genomics 10:429. 2009
  7. pmc Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases Genomic Research Laboratory, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1892-5. 2006
  8. ncbi request reprint Comparison of amplification methods for transcriptomic analyses of low abundance prokaryotic RNA sources
    Patrice Francois
    Genomic Research Laboratory, Service of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 68:385-91. 2007
  9. pmc Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 45:2011-3. 2007
  10. ncbi request reprint Genome content determination in methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Service of Infectious Diseases, Genomic Research Laboratory, Department of Internal Medicine, Geneva 14, Switzerland
    Future Microbiol 2:187-98. 2007

Detail Information

Publications66

  1. pmc Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureus
    Thibaud Koessler
    Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1040-8. 2006
    ....
  2. pmc Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
    Caroline Le Marechal
    INRA, UMR1253 Science et Technologie du Lait et de l Œuf, F 35042 Rennes, France
    Vet Res 42:35. 2011
    ..These findings open avenues in S. aureus mastitis studies as some of these proteins, expressed in vivo, are likely to account for the success of S. aureus as a pathogen of the ruminant mammary gland...
  3. pmc Genomic analysis of an emerging multiresistant Staphylococcus aureus strain rapidly spreading in cystic fibrosis patients revealed the presence of an antibiotic inducible bacteriophage
    Jean Marc Rolain
    URMITE CNRS IRD UMR 6236, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biol Direct 4:1. 2009
    ..We also conducted a retrospective epidemiological analysis on all S. aureus isolated from 2002 to 2007 in CF patients from our institution...
  4. pmc Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation
    Qiang Lou
    Key Laboratory of Medical Molecular Virology of Ministry of Education and Ministry of Public Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, 138 Yixueyuan Road, Shanghai, 200032, PR China
    BMC Microbiol 11:146. 2011
    ..The deletion of saeR in S. epidermidis results in impaired anaerobic growth and decreased nitrate utilization. However, the regulatory function of SaeRS on biofilm formation and autolysis in S. epidermidis remains unclear...
  5. pmc Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus
    Kati Seidl
    Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland
    BMC Microbiol 9:95. 2009
    ..This study is the first CcpA-dependent transcriptome and proteome analysis in Staphylococcus aureus, focussing on short-time effects of glucose under stable pH conditions...
  6. pmc Transcriptome analysis of the responses of Staphylococcus aureus to antimicrobial peptides and characterization of the roles of vraDE and vraSR in antimicrobial resistance
    Milla Pietiäinen
    Antimicrobial Resistance Unit, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare THL, PL 30, 00271 Helsinki, Finland
    BMC Genomics 10:429. 2009
    ..Three antimicrobial peptides were used in the analysis, two are derived from frog, temporin L and dermaseptin K4-S4(1-16), and the ovispirin-1 is obtained from sheep...
  7. pmc Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases Genomic Research Laboratory, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1892-5. 2006
    ..aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information...
  8. ncbi request reprint Comparison of amplification methods for transcriptomic analyses of low abundance prokaryotic RNA sources
    Patrice Francois
    Genomic Research Laboratory, Service of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 68:385-91. 2007
    ....
  9. pmc Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 45:2011-3. 2007
    ..Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones...
  10. ncbi request reprint Genome content determination in methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Service of Infectious Diseases, Genomic Research Laboratory, Department of Internal Medicine, Geneva 14, Switzerland
    Future Microbiol 2:187-98. 2007
    ..In addition, sequence information is mandatory for the development of molecular tests allowing the rapid identification, genotyping and characterization of clinical isolates...
  11. doi request reprint Rapid and high-throughput genotyping of Staphylococcus epidermidis isolates by automated multilocus variable-number of tandem repeats: a tool for real-time epidemiology
    Patrice Francois
    University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 72:296-305. 2008
    ..This type of rapid and high-throughput methodology opens the possibility to rapidly assess long-term nosocomial transmission or to characterize infecting strains in the general procedure of routine laboratories, in real-time...
  12. pmc Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005
    Patrice Francois
    University of Geneva Hospitals, Geneva, Switzerland
    Emerg Infect Dis 14:304-7. 2008
    ..We demonstrated strain diversity, including clones rarely found in Europe. Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains...
  13. ncbi request reprint Rapid bacterial identification using evanescent-waveguide oligonucleotide microarray classification
    Patrice Francois
    University Hospitals of Geneva, Genomic Research Laboratory, Service of Infectious Diseases, Switzerland
    J Microbiol Methods 65:390-403. 2006
    ..In conclusion, when coupled to optimal signal detection strategy, microarrays provide bacterial identification within a few hours post-sampling, allowing targeted antimicrobial prescription...
  14. pmc Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 43:3346-55. 2005
    ..All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time...
  15. ncbi request reprint Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens
    Patrice Francois
    Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 55:755-62. 2003
    ..Our study reveals that nanoparticle-labeled bacterial RNA generates reproducible resonance light scattering signals that are at least 50 times more intense than state-of-the-art confocal-based fluorescence signals...
  16. pmc A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 42:3309-12. 2004
    ..This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA...
  17. pmc Induction of fibronectin adhesins in quinolone-resistant Staphylococcus aureus by subinhibitory levels of ciprofloxacin or by sigma B transcription factor activity is mediated by two separate pathways
    Dongmei Li
    Service of Infectious Diseases, University Hospitals of Geneva, Geneva, Switzerland
    Antimicrob Agents Chemother 49:916-24. 2005
    ..aureus occurs via independent pathways whose concerted actions may significantly promote bacterial adhesion and colonization...
  18. pmc A generic approach for the design of whole-genome oligoarrays, validated for genomotyping, deletion mapping and gene expression analysis on Staphylococcus aureus
    Yvan Charbonnier
    Genomic Research Laboratory, University Hospitals of Geneva, Rue Micheli du Crest 24, CH 1211 Geneva 14, Switzerland
    BMC Genomics 6:95. 2005
    ..Relying on parameters shown to affect the hybridization of nucleic acids, we designed freely available software and validated experimentally its performance on the bacterial pathogen Staphylococcus aureus...
  19. pmc Modulation of fibronectin adhesins and other virulence factors in a teicoplanin-resistant derivative of methicillin-resistant Staphylococcus aureus
    Adriana Renzoni
    Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    Antimicrob Agents Chemother 48:2958-65. 2004
    ..These molecular and phenotypic changes may have a profound impact on the bacterial adhesion and colonization properties of such multiresistant organisms...
  20. pmc Transcriptomic and functional analysis of an autolysis-deficient, teicoplanin-resistant derivative of methicillin-resistant Staphylococcus aureus
    Adriana Renzoni
    Service of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    Antimicrob Agents Chemother 50:3048-61. 2006
    ..Instead, our analysis points to alternate regulatory mechanisms that impact autolysis effectors which may include changes in posttranscriptional processing or export...
  21. pmc A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells
    Christian Garzoni
    Service of Infectious Diseases, University Hospital of Geneva, Department of Internal Medicine, Geneva 14, Switzerland
    BMC Genomics 8:171. 2007
    ..Mechanisms contributing to internalization have been partly elucidated, but bacterial processes triggered intracellularly are largely unknown...
  22. pmc Identification by genomic and genetic analysis of two new genes playing a key role in intermediate glycopeptide resistance in Staphylococcus aureus
    Adriana Renzoni
    Service of Infectious Diseases, Geneva University Hospital, Geneva, Switzerland
    Antimicrob Agents Chemother 53:903-11. 2009
    ..In conclusion, our study demonstrates the key role of two novel loci in endogenous, low-level glycopeptide resistance in S. aureus whose precise molecular functions warrant further investigation...
  23. ncbi request reprint Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth
    Alexander Scherl
    Biomedical Proteomics Research Group, Geneva University Hospitals, Geneva, Switzerland
    J Microbiol Methods 60:247-57. 2005
    ..The simultaneous large-scale analysis of transcriptomes and proteomes enables a global and holistic view of the S. aureus biology, allowing the parallel study of multiple active events in an organism...
  24. pmc Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay
    Patrice Francois
    Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 41:254-60. 2003
    ..The complete protocol may provide results in less than 6 h (while standard procedure needs 2 to 3 days), thus allowing prompt and cost-effective implementation of contact precautions...
  25. pmc Daptomycin resistance mechanisms in clinically derived Staphylococcus aureus strains assessed by a combined transcriptomics and proteomics approach
    Adrien Fischer
    Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    J Antimicrob Chemother 66:1696-711. 2011
    ..The development of daptomycin resistance in Staphylococcus aureus is associated with clinical treatment failures. The mechanism(s) of such resistance have not been clearly defined...
  26. pmc High prevalence of isolates with reduced glycopeptide susceptibility in persistent or recurrent bloodstream infections due to methicillin-resistant Staphylococcus aureus
    Ilker Uckay
    Service of Infectious Diseases, Infection Control Program, Geneva University Hospital and Medical School, Geneva, Switzerland
    Antimicrob Agents Chemother 56:1258-64. 2012
    ..0001). Detection of elevated teicoplanin MICs may help to predict a poor response to glycopeptide therapy in MRSA bacteremic patients...
  27. ncbi request reprint Comparative efficacy of daptomycin and vancomycin in the therapy of experimental foreign body infection due to Staphylococcus aureus
    Pierre Vaudaux
    Division of Infectious Diseases, Geneva University Hospital, CH 1211 Geneva 14, Switzerland
    J Antimicrob Chemother 52:89-95. 2003
    ..aureus. Drug dosage should be adapted to obtain inflammatory fluid levels of daptomycin minimizing emergence of resistant subpopulations...
  28. pmc Cartography of methicillin-resistant S. aureus transcripts: detection, orientation and temporal expression during growth phase and stress conditions
    Marie Beaume
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals, Geneva, Switzerland
    PLoS ONE 5:e10725. 2010
    ..Most studies evaluating gene content have so far neglected to analyze the so-called intergenic regions as well as potential antisense RNA molecules...
  29. doi request reprint Epidemiology and virulence insights from MRSA and MSSA genome analysis
    Vladimir Lazarevic
    Genomic Research Laboratory, Geneva University Hospitals, Switzerland
    Future Microbiol 6:513-32. 2011
    ..This high resolution approach allows the monitoring micro- and macroevolution of MRSA and identification of specific genomic markers of evolution of invasive or highly virulent phenotypes...
  30. ncbi request reprint A recA-LexA-dependent pathway mediates ciprofloxacin-induced fibronectin binding in Staphylococcus aureus
    Carmelo Bisognano
    Division of Infectious Diseases, University Hospital, CH 1211 Geneva 14, Switzerland
    J Biol Chem 279:9064-71. 2004
    ..We conclude that activation of recA and derepression of lexA-regulated genes by CPX may represent a response to drug-induced damage that results in a novel induction of a virulence factor leading to increased bacterial tissue adherence...
  31. doi request reprint Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus
    Ludwig Stenz
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals, Geneva 14, Switzerland
    FEMS Microbiol Lett 287:149-55. 2008
    ..Our results suggest a natural protective effect of oleic acid against primary adhesion...
  32. doi request reprint Development and validation of a modified broad-range 16S rDNA PCR for diagnostic purposes in clinical microbiology
    Abdessalam Cherkaoui
    Clinical Microbiology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, Geneva 14, Switzerland
    J Microbiol Methods 79:227-31. 2009
    ..Compared with conventional methods, broad-range PCR with ExoIII pre-treatment of reagents substantially improves the diagnostic yield of bacterial pathogen identification from normally sterile sites...
  33. pmc Increased expression of clumping factor and fibronectin-binding proteins by hemB mutants of Staphylococcus aureus expressing small colony variant phenotypes
    Pierre Vaudaux
    Division of Infectious Diseases, University Hospital, CH 1211 Geneva 14, Switzerland
    Infect Immun 70:5428-37. 2002
    ..Both agr-positive and agr-lacking hemB mutants were also more efficiently internalized by human embryonic kidney cells than were their isogenic controls, presumably because of increased surface display of their fibronectin adhesins...
  34. ncbi request reprint Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis
    Patrice Francois
    Division of Infectious Diseases, University Hospitals of Geneva, 1211 Geneva 14, Switzerland
    FEMS Immunol Med Microbiol 35:135-40. 2003
    ..aureus or S. epidermidis were equivalent to those of their respective ica-negative mutants, in the locally infected fluids and on tissue-cage-inserted plastic coverslips...
  35. ncbi request reprint High-density DNA probe arrays for identification of staphylococci to the species level
    Sabine Couzinet
    Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli du Crest 24, 1211 Geneva 14, Switzerland
    J Microbiol Methods 61:201-8. 2005
    ..The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies...
  36. doi request reprint Proteomic approaches to study Staphylococcus aureus pathogenesis
    Patrice Francois
    Genomic Research Laboratory, Service of Infectious Diseases, Department of Internal Medicine, Geneva University and Geneva University Hospitals, 1211 Geneva 14, Switzerland
    J Proteomics 73:701-8. 2010
    ..This review summarizes the impact of proteomic on our comprehension of S. aureus virulence and pathogenesis...
  37. ncbi request reprint Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolates
    Sabine Couzinet
    Division of Infectious Diseases, University Hospitals of Geneva, CH 1211, 24 Rue Micheli du Crest, 1211 Geneva 14, Switzerland
    J Microbiol Methods 60:275-9. 2005
    ..For the two most important genes, gyrA and grlA, correlation with sequencing reached 95.1%. If all genes were considered, correlation was 88.8%...
  38. pmc Community-associated methicillin-resistant Staphylococcus aureus, Switzerland
    Stephan Harbarth
    University of Geneva Hospitals, Geneva, Switzerland
    Emerg Infect Dis 11:962-5. 2005
    ..9/1,000 admissions. Although 5 CA-MRSA isolates contained Panton-Valentine leukocidin, only 1 patient had a previous skin infection. No easily modifiable risk factor for CA-MRSA was identified...
  39. pmc Exploring glycopeptide-resistance in Staphylococcus aureus: a combined proteomics and transcriptomics approach for the identification of resistance-related markers
    Alexander Scherl
    Biomedical Proteomics Research Group, Geneva University, Geneva, Switzerland
    BMC Genomics 7:296. 2006
    ..Quantitative proteomics performed on membrane extracts showed exquisite inter-experimental reproducibility permitting the identification and relative quantification of >30% of the predicted S. aureus proteome...
  40. doi request reprint New approaches for functional genomic studies in staphylococci
    Marie Beaume
    Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals HUG, CH 1211 Geneva 14, Switzerland
    Int J Med Microbiol 300:88-97. 2010
    ..Here we review the principles, advantages and disadvantages of recent technical developments in the field of transcriptomics and their potential impact on S. aureus research...
  41. pmc Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study
    Stephan Harbarth
    Infection Control Program, Geneva University Hospitals, Geneva, Switzerland
    Crit Care 10:R25. 2006
    ....
  42. doi request reprint Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications
    Patrice Francois
    Genomic Research Laboratory, University Hospitals of Geneva, Service of Infectious Diseases, Geneva, Switzerland
    FEMS Immunol Med Microbiol 62:41-8. 2011
    ..Our results demonstrate that LAMP is not only easy to use, but is also a very robust, innovative and powerful molecular diagnostic method for both industrialized and developing countries...
  43. ncbi request reprint Proteomic approach to investigate MRSA
    Patrice Francois
    Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, Geneva, Switzerland
    Methods Mol Biol 391:179-99. 2007
    ..aureus on the organism level. Coupled with methods analyzing the whole bacterial transcriptome, this approach might contribute to the development of rapid diagnostic tests and to the identification of new drug targets...
  44. pmc Study of inter- and intra-individual variations in the salivary microbiota
    Vladimir Lazarevic
    Genomic Research Laboratory, Geneva University Hospitals, Rue Gabrielle Perret Gentil 4, CH 1211 Geneva 14, Switzerland
    BMC Genomics 11:523. 2010
    ..Culture-independent approaches provide the best means to assess the diversity of oral bacteria because most of them remain uncultivable...
  45. pmc Metagenomic study of the oral microbiota by Illumina high-throughput sequencing
    Vladimir Lazarevic
    Genomic Research Laboratory, Geneva University Hospitals, Rue Gabrielle Perret Gentil 4, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 79:266-71. 2009
    ..Access to large amounts of data is already leading to a better representation of sample diversity at a reasonable cost...
  46. pmc Novel microarray design strategy to study complex bacterial communities
    Antoine Huyghe
    Genomic Research Laboratory, Infectious Diseases Service, University of Geneva Hospitals, Micheli du Crest 24, 1211 Geneva 14, Geneva, Switzerland
    Appl Environ Microbiol 74:1876-85. 2008
    ..Our results show that this hierarchical approach can reveal minor changes (<or=1%) in gingival flora content when samples collected in individuals from similar geographical origins are compared...
  47. doi request reprint Development and evaluation of a rapid strategy to determine enterotoxin gene content in Staphylococcus aureus
    Adrien Fischer
    Genomic Research Laboratory, Service of Infectious Diseases, University Hospital of Geneva, University of Geneva, CH 1 211 Geneva 14, Switzerland
    J Microbiol Methods 77:184-90. 2009
    ..A combined genotyping method that includes rapid enterotoxin content determination appears informative for various epidemiological survey purposes...
  48. doi request reprint The CodY pleiotropic repressor controls virulence in gram-positive pathogens
    Ludwig Stenz
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals, Geneva, Switzerland
    FEMS Immunol Med Microbiol 62:123-39. 2011
    ..This is true for the anthrax toxins of Bacillus anthracis and the haemolysins of Staphylococcus aureus. The purpose of this review is to illustrate CodY-regulated mechanisms on virulence in major gram-positive pathogens...
  49. pmc Analysis of the salivary microbiome using culture-independent techniques
    Vladimir Lazarevic
    Genomic Research Laboratory, Division of Infectious Diseases, Geneva University Hospitals, Rue Gabrielle Perret Gentil 4, CH 1211 Geneva 14, Switzerland
    J Clin Bioinforma 2:4. 2012
    ..abstract:..
  50. pmc De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer
    David Hernandez
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals and the University of Geneva, CH 1211 Geneva 4, Switzerland
    Genome Res 18:802-9. 2008
    ..5- to 3.0-kb fragments. We also provide indications that the broad coverage achieved by high-throughput sequencing might allow for the detection of clonal polymorphisms in the set of DNA molecules being sequenced...
  51. pmc Comparison of levofloxacin, alatrofloxacin, and vancomycin for prophylaxis and treatment of experimental foreign-body-associated infection by methicillin-resistant Staphylococcus aureus
    Pierre Vaudaux
    Division of Infectious Diseases, Geneva University Hospital, CH 1211 Geneva 14, Switzerland
    Antimicrob Agents Chemother 46:1503-9. 2002
    ..The mechanisms explaining the inferior activity of alatrofloxacin compared to the activity of levofloxacin against chronic foreign-body-associated infections by MRSA are unknown...
  52. ncbi request reprint Comparison of four chromogenic media for culture-based screening of meticillin-resistant Staphylococcus aureus
    Abdessalam Cherkaoui
    Clinical Microbiology Laboratory, University of Geneva Hospitals HUG, Service of Infectious Diseases, CH 1211 Geneva 14, Switzerland
    J Med Microbiol 56:500-3. 2007
    ..aureus are commercially available. However, their respective performances under real conditions of utilization are heterogeneous, underlining the absence of a gold standard medium for MRSA screening...
  53. pmc Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level
    Abdessalam Cherkaoui
    Clinical Microbiology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 48:1169-75. 2010
    ..Our data suggest that implementation of MS as a first test strategy for one-step species identification would improve timeliness and reduce isolate identification costs in clinical bacteriology laboratories now...
  54. pmc Screening for staphylococcal superantigen genes shows no correlation with the presence or the severity of chronic rhinosinusitis and nasal polyposis
    Frédéric Heymans
    Rhinology Olfactology Unit, Department of Otorhinolaryngology and ENT Surgery, Geneva University Hospital, Geneva, Switzerland
    PLoS ONE 5:e9525. 2010
    ..The aim of this study was to investigate whether the presence of any of the 22 currently known staphylococcal exotoxin genes could be correlated with chronic rhinosinusitis...
  55. doi request reprint CC9 livestock-associated Staphylococcus aureus emerges in bloodstream infections in French patients unconnected with animal farming
    Cindy Lamamy
    Genomic Research Laboratory, University of Geneva Hospitals, Switzerland
    Clin Infect Dis 56:e83-6. 2013
    ..We demonstrate, by microarray analysis, the presence of egc cluster, fnbA, cap operon, lukS, set2, set12, splE, splD, sak, epiD, and can, genomic features associated with a high virulence potential in humans...
  56. pmc Comparison of DNA extraction methods in analysis of salivary bacterial communities
    Vladimir Lazarevic
    Genomic Research Laboratory, Division of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland
    PLoS ONE 8:e67699. 2013
    ..Differences between the extraction procedures outweighed the variations due to the bioinformatics analysis pipelines used. ..
  57. doi request reprint Accelerated digestion for high-throughput proteomics analysis of whole bacterial proteomes
    Ali R Vaezzadeh
    Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, Geneva University, 1 Michel Servet, Geneva, Switzerland
    J Microbiol Methods 80:56-62. 2010
    ..The combination of high-throughput proteomics with accelerated and efficient sample preparation should enhance the practicability of proteomics by reducing the time from sample collection to obtaining the results...
  58. doi request reprint Differentiating osteoarticular infections caused by Kingella kingae from those due to typical pathogens in young children
    Dimitri Ceroni
    Pediatric Orthopedic Service, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland
    Pediatr Infect Dis J 30:906-9. 2011
    ..kingae osteoarticular infections by using the following 4 variables: body temperature <38°C, serum C-reactive protein <55 mg/L, white blood cell count <14,000/mm, and band forms <150/mm...
  59. doi request reprint Development of a rapid PCR assay for screening of maternal colonization by group B streptococcus and neonatal invasive Escherichia coli during labor
    Begona Martinez de Tejada
    Department of Obstetrics and Gynecology, University Hospitals of Geneva, Geneva, Switzerland
    Gynecol Obstet Invest 70:250-5. 2010
    ..To screen for colonization during labor, we developed a real-time PCR assay for the simultaneous detection of GBS and neonatal invasive strains of E. coli...
  60. doi request reprint Orientation and expression of methicillin-resistant Staphylococcus aureus small RNAs by direct multiplexed measurements using the nCounter of NanoString technology
    Marie Beaume
    Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland
    J Microbiol Methods 84:327-34. 2011
    ..e. during host-pathogen interactions) or when starting from poorly purified RNA or even directly from lysed infected tissues...
  61. doi request reprint Bacterial genome evolution within a clonal population: from in vitro investigations to in vivo observations
    Marie Beaume
    Genomic Research Laboratory, Infectious Diseases Service, University of Geneva Hospitals, Rue Gabrielle Perret Gentil 4, CH 1211 Geneva 14, Switzerland
    Future Microbiol 8:661-74. 2013
    ..In this review, the authors discuss the evolution of bacteria in the laboratory and in human patients...
  62. pmc In vivo detection of clonally derived methicillin-resistant/methicillin-susceptible Staphylococcus aureus strains is not a rare event
    Patrice Francois
    J Clin Microbiol 46:1890-1. 2008
  63. doi request reprint Ceftazidime-resistant Klebsiella oxytoca producing an OXY-2-type variant from Switzerland
    José Manuel Rodríguez-Martínez
    Int J Antimicrob Agents 32:278-9. 2008
  64. ncbi request reprint Evidence of an intracellular reservoir in the nasal mucosa of patients with recurrent Staphylococcus aureus rhinosinusitis
    Sophie Clement
    Department of Pathology and Immunology, University of Geneva, Switzerland
    J Infect Dis 192:1023-8. 2005
    ..Intracellular residence may provide a sanctuary for pathogenic bacteria by protecting them from host defense mechanisms and antibiotic treatment during acute, recurrent S. aureus rhinosinusitis...
  65. ncbi request reprint Nonredundant mass spectrometry: a strategy to integrate mass spectrometry acquisition and analysis
    Alexander Scherl
    Biomedical Proteomics Research Group, Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland
    Proteomics 4:917-27. 2004
    ..It was also found that this new workflow significantly increased sequence coverage by identifying unexpected peptides, which can result from post-translational modifications...
  66. ncbi request reprint Inactivation of a subpopulation of human neutrophils by exposure to ultrahigh-molecular-weight polyethylene wear debris
    Louis Bernard
    Service of Orthopaedic Surgery, Geneva University Hospital, Switzerland
    FEMS Immunol Med Microbiol 49:425-32. 2007
    ..In conclusion, engulfment of UHMWPE wear particles led to inactivation of S. aureus uptake in nearly half of the neutrophil population, which may potentially impair host clearance mechanisms against pyogenic infections...