Witold Filipowicz

Summary

Affiliation: Friedrich Miescher Institute
Country: Switzerland

Publications

  1. ncbi request reprint The liver-specific microRNA miR-122: biology and therapeutic potential
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, Basel CH 4002, Switzerland
    Prog Drug Res 67:221-38. 2011
  2. doi request reprint Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Nat Rev Genet 9:102-14. 2008
  3. ncbi request reprint Biogenesis of small nucleolar ribonucleoproteins
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Curr Opin Cell Biol 14:319-27. 2002
  4. ncbi request reprint Single processing center models for human Dicer and bacterial RNase III
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 118:57-68. 2004
  5. pmc TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing
    Astrid D Haase
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4508 Basel, Switzerland
    EMBO Rep 6:961-7. 2005
  6. pmc The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
    Inga Loedige
    Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
    Nucleic Acids Res 41:518-32. 2013
  7. pmc miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs
    Marina Chekulaeva
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Nat Struct Mol Biol 18:1218-26. 2011
  8. pmc Dicer is associated with ribosomal DNA chromatin in mammalian cells
    Lasse Sinkkonen
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    PLoS ONE 5:e12175. 2010
  9. pmc Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells
    Daniela Schmitter
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002 Basel, Switzerland
    Nucleic Acids Res 34:4801-15. 2006
  10. pmc The double-stranded RNA binding domain of human Dicer functions as a nuclear localization signal
    Michael Doyle
    Friedrich Miescher Institute for Biomedical Research, CH 4058 Basel, Switzerland
    RNA 19:1238-52. 2013

Collaborators

Detail Information

Publications43

  1. ncbi request reprint The liver-specific microRNA miR-122: biology and therapeutic potential
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, Basel CH 4002, Switzerland
    Prog Drug Res 67:221-38. 2011
    ..We discuss antisense strategies targeting miR-122 as a potential therapeutic approach to treat hepatitis C and possibly other diseases...
  2. doi request reprint Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Nat Rev Genet 9:102-14. 2008
    ..This Review summarizes the current understanding of the mechanistic aspects of microRNA-induced repression of translation and discusses some of the controversies regarding different modes of microRNA function...
  3. ncbi request reprint Biogenesis of small nucleolar ribonucleoproteins
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Curr Opin Cell Biol 14:319-27. 2002
    ..Cajal bodies emerge as nuclear structures important for the biogenesis and function of small nucleolar ribonucleoproteins...
  4. ncbi request reprint Single processing center models for human Dicer and bacterial RNase III
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 118:57-68. 2004
    ..Based on these and other data, we propose that Dicer functions through intramolecular dimerization of its two RNase III domains, assisted by the flanking RNA binding domains, PAZ and dsRBD...
  5. pmc TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing
    Astrid D Haase
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4508 Basel, Switzerland
    EMBO Rep 6:961-7. 2005
    ..The TRBP-Dicer interaction shown raises interesting questions about the potential interplay between RNAi and interferon-PKR pathways...
  6. pmc The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
    Inga Loedige
    Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
    Nucleic Acids Res 41:518-32. 2013
    ....
  7. pmc miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs
    Marina Chekulaeva
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Nat Struct Mol Biol 18:1218-26. 2011
    ....
  8. pmc Dicer is associated with ribosomal DNA chromatin in mammalian cells
    Lasse Sinkkonen
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    PLoS ONE 5:e12175. 2010
    ..Components of the RNA silencing machinery are involved in different aspects of chromatin function in numerous organisms. However, association of RNA silencing with chromatin in mammalian cells remains unclear...
  9. pmc Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells
    Daniela Schmitter
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002 Basel, Switzerland
    Nucleic Acids Res 34:4801-15. 2006
    ..Taken together, our data suggest that, independent of the effect on translation, miRNAs affect levels of a few hundred mRNAs in HEK293 cells...
  10. pmc The double-stranded RNA binding domain of human Dicer functions as a nuclear localization signal
    Michael Doyle
    Friedrich Miescher Institute for Biomedical Research, CH 4058 Basel, Switzerland
    RNA 19:1238-52. 2013
    ..Finally, we demonstrate that human Dicer has the ability to shuttle between the nucleus and the cytoplasm. We conclude that Dicer is a shuttling protein whose steady-state localization is cytoplasmic. ..
  11. doi request reprint MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells
    Lasse Sinkkonen
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Nat Struct Mol Biol 15:259-67. 2008
    ..The defective DNA methylation can be rescued by ectopic expression of de novo Dnmts or by transfection of the miR-290 cluster miRNAs, indicating that de novo DNA methylation in ES cells is controlled by miRNAs...
  12. ncbi request reprint Inhibition of translational initiation by Let-7 MicroRNA in human cells
    Ramesh S Pillai
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Science 309:1573-6. 2005
    ..Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression...
  13. ncbi request reprint Post-transcriptional gene silencing by siRNAs and miRNAs
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Curr Opin Struct Biol 15:331-41. 2005
    ..The first structural information on protein components of the RNA interference (RNAi) and miRNA machineries is emerging, and provides some insight into the mechanism of RNA-silencing reactions...
  14. pmc Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells
    Julien Béthune
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    EMBO Rep 13:716-23. 2012
    ..This step is followed by mRNA deadenylation and decay, which is the dominant effect at steady state. Our findings have important implications for understanding the mechanism of silencing and reconcile seemingly contradictory data...
  15. ncbi request reprint Relief of microRNA-mediated translational repression in human cells subjected to stress
    Suvendra N Bhattacharyya
    Friedrich Miescher Institute for Biomedical Research, P O Box 2543, 4002 Basel, Switzerland
    Cell 125:1111-24. 2006
    ..We propose that proteins interacting with the 3'UTR will generally act as modifiers altering the potential of miRNAs to repress gene expression...
  16. ncbi request reprint Argonautes and company: sailing against the wind
    Suvendra N Bhattacharyya
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Cell 128:1027-8. 2007
    ..AGO2 and FXR1 bind to the AU-rich element in the 3'-UTR of TNFalpha mRNA, unexpectedly activating its translation in a cell growth-dependent manner...
  17. ncbi request reprint Human dicer: purification, properties, and interaction with PAZ PIWI domain proteins
    Fabrice A Kolb
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Methods Enzymol 392:316-36. 2005
    ..In this chapter we describe overexpression and purification of recombinant human Dicer, its biochemical properties, and mapping of domains responsible for Dicer-PPD protein interactions...
  18. ncbi request reprint Role of Dicer in posttranscriptional RNA silencing
    Lukasz Jaskiewicz
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Curr Top Microbiol Immunol 320:77-97. 2008
    ..We also discuss information about Dicer-associated proteins and their role in the biogenesis of small RNAs and assembly of RISC...
  19. pmc The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression
    Marina Chekulaeva
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Nucleic Acids Res 38:6673-83. 2010
    ..These results demonstrate a novel role for the GW/WG repeats as effector motifs in miRNA-mediated repression...
  20. pmc Importance of the C-terminal domain of the human GW182 protein TNRC6C for translational repression
    Jakob T Zipprich
    Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
    RNA 15:781-93. 2009
    ..Through deletion and mutagenesis, we identified the C-terminal part of TNRC6C encompassing the RRM RNA-binding motif as a key effector domain mediating protein synthesis repression by TNRC6C...
  21. doi request reprint Proteomics joins the search for microRNA targets
    Helge Grosshans
    Friedrich Miescher Institute for Biomedical Research, CH 4002 Basel, Switzerland
    Cell 134:560-2. 2008
    ..This approach is a powerful means to identify miRNA targets and to quantify the contribution of translational repression to posttranscriptional gene silencing by miRNAs...
  22. pmc Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis
    Ramesh S Pillai
    Friedrich Miescher Institute for Biomedical Research, P O Box 2543, 4002 Basel, Switzerland
    RNA 10:1518-25. 2004
    ..These findings indicate that a primary function of miRNAs is to guide their associated proteins to the mRNA...
  23. doi request reprint The widespread regulation of microRNA biogenesis, function and decay
    Jacek Krol
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Nat Rev Genet 11:597-610. 2010
    ..Such regulation has an important role in the context-specific functions of miRNAs...
  24. pmc Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila
    Marina Chekulaeva
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    RNA 15:794-803. 2009
    ..Each of these domains also functions independently of poly(A) tails. These results indicate that miRNA-mediated repression is facilitated by multiple domains of GW182...
  25. pmc HuR protein attenuates miRNA-mediated repression by promoting miRISC dissociation from the target RNA
    Pradipta Kundu
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Nucleic Acids Res 40:5088-100. 2012
    ..Further, we demonstrate that HuR association with AREs can also inhibit miRNA-mediated deadenylation of mRNA in the Krebs-2 ascites extract, in a manner likewise depending on the potential of HuR to oligomerize...
  26. doi request reprint Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells
    Marina Chekulaeva
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Curr Opin Cell Biol 21:452-60. 2009
    ..In this report, we review current progress in understanding how miRNAs execute these effects in animals and we discuss some of the controversies regarding different modes of miRNA function...
  27. ncbi request reprint RNAi: the nuts and bolts of the RISC machine
    Witold Filipowicz
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Cell 122:17-20. 2005
    ....
  28. ncbi request reprint Properties of the regulatory RNA-binding protein HuR and its role in controlling miRNA repression
    Nicole Claudia Meisner
    Novartis Institutes for BioMedical Research, Novartis Campus, 4002 Basel, Switzerland
    Adv Exp Med Biol 700:106-23. 2010
    ..In this chapter we review properties of HuR and describe examples of the cross-talk between the protein and miRNAs, with emphasis on response of the regulation to cellular stress...
  29. pmc Lack of homologous sequence-specific DNA methylation in response to stable dsRNA expression in mouse oocytes
    Petr Svoboda
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Nucleic Acids Res 32:3601-6. 2004
    ..These data demonstrate that dsRNA does not directly induce DNA methylation in the trans form of this sequence in these mammalian cells...
  30. doi request reprint Characterizing light-regulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs
    Jacek Krol
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 141:618-31. 2010
    ..Our results demonstrate that microRNA metabolism in neurons is higher than in most other cells types and linked to neuronal activity...
  31. pmc dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells
    Jana Nejepinska
    Institute of Molecular Genetics AS CR, Videnska 1083, 14220 Prague 4, Czech Republic
    Nucleic Acids Res 40:399-413. 2012
    ..RNA editing neither prevented the cytoplasmic localization nor processing into siRNAs. Thus, a long dsRNA structure is well tolerated in mammalian cells and is mainly causing a robust RNAi response in oocytes...
  32. pmc UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants
    Mark H L Lambermon
    Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
    Mol Cell Biol 22:4346-57. 2002
    ..These and other data suggest that UBP1, UBA1a, and UBA2a may act as components of a complex recognizing U-rich sequences in plant 3'-UTRs and contributing to the stabilization of mRNAs in the nucleus...
  33. pmc Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    EMBO J 21:5875-85. 2002
    ..Additional experiments suggest that if ATP participates in the Dicer reaction in mammalian cells, it might be involved in product release needed for the multiple turnover of the enzyme...
  34. ncbi request reprint MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F
    Geraldine Mathonnet
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada, H3G 1Y6
    Science 317:1764-7. 2007
    ..Our results indicate that inhibition of translation initiation is the earliest molecular event effected by miRNAs. Other mechanisms, such as mRNA degradation, may subsequently consolidate mRNA silencing...
  35. pmc ATP modulates siRNA interactions with an endogenous human Dicer complex
    Janice L Pellino
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
    RNA 11:1719-24. 2005
    ..Complex D could impinge upon the RISC assembly pathway in humans, similar to an analogous complex in Drosophila...
  36. doi request reprint Molecular biology: the expanding world of small RNAs
    Helge Grosshans
    Nature 451:414-6. 2008
  37. pmc The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components
    Noora Kotaja
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, B P 10142, 67404 Illkirch Strasbourg, France
    Proc Natl Acad Sci U S A 103:2647-52. 2006
    ..Our findings underscore the importance of posttranscriptional gene regulation and of the microRNA pathway in the control of postmeiotic male germ cell differentiation...
  38. pmc Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer
    Nasser Tahbaz
    Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada
    EMBO Rep 5:189-94. 2004
    ..Lastly, we show that PPD proteins and Dicer are present in soluble and membrane-associated fractions, indicating that interactions between these two types of proteins may occur in multiple compartments...
  39. ncbi request reprint Repression of protein synthesis by miRNAs: how many mechanisms?
    Ramesh S Pillai
    European Molecular Biology Laboratory, Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
    Trends Cell Biol 17:118-26. 2007
    ..Recent data support different microRNA mechanisms and a role for cytoplasmic processing bodies in the degradation and storage of mRNAs targeted by microRNA regulators...
  40. ncbi request reprint Crystal structures of the semireduced and inhibitor-bound forms of cyclic nucleotide phosphodiesterase from Arabidopsis thaliana
    Andreas Hofmann
    Macromolecular Crystallography Laboratory, NCI, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 277:1419-25. 2002
    ..The ligand is bound within the active site, and the mode of binding is in agreement with the previously proposed enzymatic mechanism. Selected biophysical properties of the oxidized and the semireduced CPDase are also discussed...
  41. pmc Interferon signaling and treatment outcome in chronic hepatitis C
    Magdalena Sarasin-Filipowicz
    Department of Biomedicine, University of Basel, CH 4031 Basel, Switzerland
    Proc Natl Acad Sci U S A 105:7034-9. 2008
    ....
  42. ncbi request reprint From RNAi to epigenomes: how RNA rules the world
    Eric Westhof
    Institut de Biologie Moleculaire et Cellulaire du CNRS, Universite Louis Pasteur, 15 rue Rene Descartes, 67084 Strasbourg, France
    Chembiochem 6:441-3. 2005
  43. pmc A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs
    Iwan Beuvink
    Novartis Institutes for BioMedical Research, Genome and Proteome Sciences, Basel, Switzerland
    Nucleic Acids Res 35:e52. 2007
    ....