Giuseppe Pantaleo

Summary

Affiliation: Centre Hospitalier Universitaire Vaudois
Country: Switzerland

Publications

  1. ncbi request reprint Correlates of immune protection in HIV-1 infection: what we know, what we don't know, what we should know
    Giuseppe Pantaleo
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    Nat Med 10:806-10. 2004
  2. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
  3. pmc Evidence for rapid disappearance of initially expanded HIV-specific CD8+ T cell clones during primary HIV infection
    G Pantaleo
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:9848-53. 1997
  4. pmc Rapid perturbation in viremia levels drives increases in functional avidity of HIV-specific CD8 T cells
    Selena Vigano
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland
    PLoS Pathog 9:e1003423. 2013
  5. ncbi request reprint Strong EBV-specific CD8+ T-cell response in patients with early multiple sclerosis
    Samantha Jilek
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Brain 131:1712-21. 2008
  6. pmc Monitoring of CD4+CD25highIL-7Rαhigh activated T cells in kidney transplant recipients
    Laure Vallotton
    Service of Transplantation, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 46, 1011 Lausanne, Switzerland
    Clin J Am Soc Nephrol 6:2025-33. 2011
  7. doi request reprint Intrathecal immune responses to EBV in early MS
    Emilie Jaquiéry
    Service of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Eur J Immunol 40:878-87. 2010
  8. pmc HIV-1-specific IFN-gamma/IL-2-secreting CD8 T cells support CD4-independent proliferation of HIV-1-specific CD8 T cells
    Simone C Zimmerli
    Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 102:7239-44. 2005
  9. doi request reprint Cytokine mRNA profile of Epstein-Barr virus-stimulated highly differentiated T cells in multiple sclerosis: a pilot study
    Emilie Jaquiéry
    Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Neuroimmunol 225:167-70. 2010
  10. ncbi request reprint NYVAC immunization induces polyfunctional HIV-specific T-cell responses in chronically-infected, ART-treated HIV patients
    Alexandre Harari
    Division of Immunology and Allergy, Lausanne University Hospital, Lausanne, Switzerland
    Eur J Immunol 42:3038-48. 2012

Research Grants

  1. South-West European HIV Vaccine Clinical Trial Unit
    Giuseppe Pantaleo; Fiscal Year: 2007
  2. South-West European HIV Vaccine Clinical Trial Unit
    Giuseppe Pantaleo; Fiscal Year: 2008

Detail Information

Publications83

  1. ncbi request reprint Correlates of immune protection in HIV-1 infection: what we know, what we don't know, what we should know
    Giuseppe Pantaleo
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    Nat Med 10:806-10. 2004
    ..We will also discuss why it is important to design phase 3 clinical trials of HIV vaccines to determine the correlates of protection for each individual vaccine...
  2. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
    ....
  3. pmc Evidence for rapid disappearance of initially expanded HIV-specific CD8+ T cell clones during primary HIV infection
    G Pantaleo
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:9848-53. 1997
    ..These findings should provide insights into how HIV, and possibly other viruses, elude the host immune response during primary infection...
  4. pmc Rapid perturbation in viremia levels drives increases in functional avidity of HIV-specific CD8 T cells
    Selena Vigano
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland
    PLoS Pathog 9:e1003423. 2013
    ..These results provide insights on the relationships between functional avidity, viremia, T-cell exhaustion and TCR renewal of antiviral CD8 T cell responses. ..
  5. ncbi request reprint Strong EBV-specific CD8+ T-cell response in patients with early multiple sclerosis
    Samantha Jilek
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Brain 131:1712-21. 2008
    ..Our data show high levels of CD8+ T-cell activation against EBV--but not CMV--early in the course of MS, which support the hypothesis that EBV might be associated with the onset of this disease...
  6. pmc Monitoring of CD4+CD25highIL-7Rαhigh activated T cells in kidney transplant recipients
    Laure Vallotton
    Service of Transplantation, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 46, 1011 Lausanne, Switzerland
    Clin J Am Soc Nephrol 6:2025-33. 2011
    ....
  7. doi request reprint Intrathecal immune responses to EBV in early MS
    Emilie Jaquiéry
    Service of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Eur J Immunol 40:878-87. 2010
    ..Our data, showing a high EBV-, but not CMV-specific intrathecal immune response, strengthen the association between EBV and MS, in particular at the onset of the disease...
  8. pmc HIV-1-specific IFN-gamma/IL-2-secreting CD8 T cells support CD4-independent proliferation of HIV-1-specific CD8 T cells
    Simone C Zimmerli
    Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 102:7239-44. 2005
    ..These results indicate that IFN-gamma/IL-2-secreting CD8 T cells may promote antigen-specific proliferation of CD8 T cells even in the absence of helper CD4 T cells...
  9. doi request reprint Cytokine mRNA profile of Epstein-Barr virus-stimulated highly differentiated T cells in multiple sclerosis: a pilot study
    Emilie Jaquiéry
    Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Neuroimmunol 225:167-70. 2010
    ..All the other tested molecules were expressed similarly in MS patients and HC. Interestingly, increased IFN-gamma and IL-4 suggest that the control of EBV replication may be insufficient in MS patients...
  10. ncbi request reprint NYVAC immunization induces polyfunctional HIV-specific T-cell responses in chronically-infected, ART-treated HIV patients
    Alexandre Harari
    Division of Immunology and Allergy, Lausanne University Hospital, Lausanne, Switzerland
    Eur J Immunol 42:3038-48. 2012
    ..In conclusion, NYVAC-B immunization induces broad, vigorous, and polyfunctional HIV-specific T-cell responses, suggesting that poxvirus-based vaccine regimens may be instrumental in the therapeutic HIV vaccine field...
  11. ncbi request reprint Distribution and functional analysis of memory antiviral CD8 T cell responses in HIV-1 and cytomegalovirus infections
    Kim Ellefsen
    Laboratory of AIDS Immunopathogenesis, Divisions of Immunology and Allergy and Infectious Diseases, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Eur J Immunol 32:3756-64. 2002
    ..Taken together, these results provide further advances in the characterization of human memory CD8 T cells...
  12. pmc Skewed association of polyfunctional antigen-specific CD8 T cell populations with HLA-B genotype
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 104:16233-8. 2007
    ..These results provide the rationale for the observed protective role of HLA-B in HIV-1-infection and new insights into the relationship between TCR avidity, PD-1 expression, and the functional profile of CD8 T cells...
  13. ncbi request reprint Functional patterns of HIV-1-specific CD4 T-cell responses in children are influenced by the extent of virus suppression and exposure
    Rafael Correa
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    AIDS 21:23-30. 2007
    ..HIV-1-specific IL-2-secreting CD4 T cells are key components of effective immunity...
  14. ncbi request reprint Functional heterogeneity of memory CD4 T cell responses in different conditions of antigen exposure and persistence
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Immunol 174:1037-45. 2005
    ..The results obtained supported the hypothesis that the different patterns observed were substantially influenced by different conditions of Ag exposure and persistence...
  15. pmc Proliferation capacity and cytotoxic activity are mediated by functionally and phenotypically distinct virus-specific CD8 T cells defined by interleukin-7R{alpha} (CD127) and perforin expression
    Cristina Cellerai
    Laboratory of AIDS Immunopathogenesis, Service of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Virol 84:3868-78. 2010
    ..These results advance our understanding of the relationship between cytotoxicity, proliferation capacity, the levels of senescence and exhaustion, and antigen exposure of antiviral memory CD8 T cells...
  16. doi request reprint Polyfunctional HCV-specific T-cell responses are associated with effective control of HCV replication
    Donatella Ciuffreda
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, CHUV, Lausanne, Switzerland
    Eur J Immunol 38:2665-77. 2008
    ..Therefore, the present results suggest that immune-based mechanisms may contribute to explain the accelerated course of HCV infection in conditions of HIV-1 co-infection and liver transplantation...
  17. pmc Distinct profiles of cytotoxic granules in memory CD8 T cells correlate with function, differentiation stage, and antigen exposure
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Virol 83:2862-71. 2009
    ..These results advance our understanding of the relationship between distinct profiles of cytotoxic granules in memory CD8 T cells and function, differentiation stage, and antigen exposure...
  18. doi request reprint Hepatitis C virus infection after liver transplantation is associated with lower levels of activated CD4(+)CD25(+)CD45RO(+)IL-7ralpha(high) T cells
    Donatella Ciuffreda
    Division of Immunology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Liver Transpl 16:49-55. 2010
    ....
  19. ncbi request reprint Functional signatures of protective antiviral T-cell immunity in human virus infections
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Immunol Rev 211:236-54. 2006
    ..e. IFN-gamma secretion, CD4+ and CD8+ T-cell responses represent correlates of protective antiviral immunity in chronic virus infections...
  20. pmc An HIV-1 clade C DNA prime, NYVAC boost vaccine regimen induces reliable, polyfunctional, and long-lasting T cell responses
    Alexandre Harari
    Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    J Exp Med 205:63-77. 2008
    ..These results support the development of the poxvirus platform in the HIV vaccine field and the further clinical development of the DNA C plus NYVAC C vaccine regimen...
  21. ncbi request reprint Cytomegalovirus (CMV)-specific cellular immune responses
    Alexandre Harari
    Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Hum Immunol 65:500-6. 2004
    ..Furthermore, it has also been shown that the combination of virus-specific CD4 and CD8 T-cell responses are critical components of the immune response in the control of virus replication...
  22. ncbi request reprint Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland
    Blood 100:1381-7. 2002
    ..These results advance the characterization of antiviral memory CD4 T-cell response and the delineation of the potential mechanisms that likely prevent the generation of a robust CD4 T-cell immune response during primary infection...
  23. ncbi request reprint Immune responses to JC virus in patients with multiple sclerosis treated with natalizumab: a cross-sectional and longitudinal study
    Samantha Jilek
    Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Lancet Neurol 9:264-72. 2010
    ..We aimed to better understand the associations between JC virus, which causes PML, and natalizumab treatment...
  24. pmc Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells
    Carsten Krieg
    Division of Immunology and Allergy, University Hospital of Lausanne, CH 1011 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 107:11906-11. 2010
    ..Thus, IL-2/anti-IL-2 antibody complexes might improve current strategies of IL-2-based tumor immunotherapy...
  25. pmc Expansion and tissue infiltration of an allospecific CD4+CD25+CD45RO+IL-7Ralphahigh cell population in solid organ transplant recipients
    Laura Codarri
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, University of Lausanne, 1011 Lausanne, Switzerland
    J Exp Med 204:1533-41. 2007
    ..These results indicate that the CD4(+)CD25(+)IL-7Ralpha(+) cell population may represent a valuable, sensitive, and specific marker to monitor allospecific CD4 T cell responses both in blood and in tissues after organ transplantation...
  26. ncbi request reprint EV01: a phase I trial in healthy HIV negative volunteers to evaluate a clade C HIV vaccine, NYVAC-C undertaken by the EuroVacc Consortium
    Pierre Alexandre Bart
    Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Vaccine 26:3153-61. 2008
    ..Half of the 10 assessed responded in the ELISpot assay under stringent criteria, which informed the sample size for a DNA-NYVAC-C comparison to NYVAC-C alone...
  27. pmc Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production
    Matthieu Perreau
    Divisions of Immunology and Allergy, Lausanne University Hospital, University of Lausanne, CH 1011 Lausanne, Switzerland
    J Exp Med 210:143-56. 2013
    ..However, only the percentage of Tfh cells correlated with the levels of plasma viremia. These results demonstrate that Tfh cells serve as the major CD4 T cell compartment for HIV infection, replication, and production...
  28. doi request reprint Nonrandom distribution of cryptic repeating triplets of purines and pyrimidines (RNY)(n) in gp120 of HIV Type1
    Elisa De Crignis
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, CHUV, Lausanne, Switzerland
    AIDS Res Hum Retroviruses 28:493-504. 2012
    ..These data suggest that cryptic repeats of RNY may play a role in the genesis of multiple base insertions and deletions in hypervariable regions of gp120...
  29. ncbi request reprint HIV-1 drug resistance transmission networks in southwest Switzerland
    Erika Castro
    Service of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Switzerland
    AIDS Res Hum Retroviruses 26:1233-8. 2010
    ..These findings have relevant implications for the local molecular mapping of HIV-1 and future ART surveillance studies in the region...
  30. ncbi request reprint Skewed representation of functionally distinct populations of virus-specific CD4 T cells in HIV-1-infected subjects with progressive disease: changes after antiretroviral therapy
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Rue Bugnon, 1011 Lausanne, Switzerland
    Blood 103:966-72. 2004
    ..These results indicate that the composition of the pool of functionally distinct virus-specific CD4 T cells is important for virus control...
  31. pmc IL-2/anti-IL-2 antibody complexes show strong biological activity by avoiding interaction with IL-2 receptor alpha subunit CD25
    Sven Létourneau
    Division of Immunology and Allergy, University Hospital of Lausanne, CH 1011 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 107:2171-6. 2010
    ..These data suggest an unpredicted role for CD25 in IL-2 homeostasis...
  32. ncbi request reprint Antiviral memory T cell responses: correlation with protective immunity and implication for vaccine development
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Divisions of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland
    Adv Exp Med Biol 512:155-64. 2002
  33. ncbi request reprint Functional and phenotypic characterization of tetanus toxoid-specific human CD4+ T cells following re-immunization
    Cristina Cellerai
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Eur J Immunol 37:1129-38. 2007
    ..Together, these results suggest that IL-7Ralpha expression levels might be a useful marker for identifying long-lived Ag-specific CD4(+ )T cells in memory T cells...
  34. pmc Early and prolonged antiretroviral therapy is associated with an HIV-1-specific T-cell profile comparable to that of long-term non-progressors
    Cristina Cellerai
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    PLoS ONE 6:e18164. 2011
    ..We have therefore hypothesized that ART maintenance over a very prolonged period in HIV-1 seroconverters could induce an immuno-virological status similar to that of HIV-1 long-term non-progressors (LTNPs)...
  35. pmc DNA/NYVAC vaccine regimen induces HIV-specific CD4 and CD8 T-cell responses in intestinal mucosa
    Matthieu Perreau
    Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Virol 85:9854-62. 2011
    ....
  36. ncbi request reprint Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease
    Virginie Rozot
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Eur J Immunol 43:1568-77. 2013
    ..Thus, the activity of Mtb infection and clinical presentation are associated with distinct profiles of Mtb-specific CD8(+) T-cell responses. These results provide new insights in the interaction between Mtb and the host immune response...
  37. doi request reprint Impairment of JCV-specific T-cell response by corticotherapy: effect on PML-IRIS management?
    Caroline Antoniol
    Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Neurology 79:2258-64. 2012
    ....
  38. ncbi request reprint HLA-B7-restricted EBV-specific CD8+ T cells are dysregulated in multiple sclerosis
    Samantha Jilek
    Division of Immunology and Allergy, Department of Medicine, University Hospital of Lausanne, 1011 Lausanne, Switzerland
    J Immunol 188:4671-80. 2012
    ..This observation is particularly interesting knowing that the HLA-B7 allele is more frequently expressed in MS patients and considering that EBV is associated with MS...
  39. ncbi request reprint Phenotypic heterogeneity of antigen-specific CD4 T cells under different conditions of antigen persistence and antigen load
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Eur J Immunol 34:3525-33. 2004
    ..The present results indicate that the heterogeneity of different Ag-specific CD4 T cell responses is regulated by Ag exposure and Ag load...
  40. ncbi request reprint De novo T-cell generation in patients at different ages and stages of HIV-1 disease
    Massimo Nobile
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Rue Bugnon, 1011 Lausanne, Switzerland
    Blood 104:470-7. 2004
    ..Our results demonstrate a dichotomy between TREC contents in CD4 and CD8 T-cell populations in HIV-1 infection and indicate that thymus function in younger subjects is preserved at early and intermediate stages of HIV infection...
  41. ncbi request reprint Differences in HCV-specific T cell responses between chronic HCV infection and HIV/HCV co-infection
    Valerie Dutoit
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Eur J Immunol 35:3493-504. 2005
    ..Altogether, these results provide new insights into the characterization of HCV-specific T cell responses in HCV mono-infected and HIV/HCV co-infected individuals...
  42. doi request reprint Poxvirus vector-based HIV vaccines
    Giuseppe Pantaleo
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland
    Curr Opin HIV AIDS 5:391-6. 2010
    ....
  43. doi request reprint A soluble hexameric form of CD40 ligand activates human dendritic cells and augments memory T cell response
    Isabelle Miconnet
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, 1011 Lausanne, Switzerland
    Vaccine 26:4006-14. 2008
    ..These results demonstrate that this soluble form of CD40 ligand may serve as an adjuvant for T cell response and thus provide the rationale for its potential use in T cell based vaccine strategies...
  44. pmc Activation of a dendritic cell-T cell axis by Ad5 immune complexes creates an improved environment for replication of HIV in T cells
    Matthieu Perreau
    Department of Medicine, Service of Immunology and Allergy, Centre Hospitalier, Universitaire Vaudois, Lausanne, University of Lausanne, Lausanne, Switzerland
    J Exp Med 205:2717-25. 2008
    ....
  45. pmc c-Cbl expression levels regulate the functional responses of human central and effector memory CD4 T cells
    Nicolò C Brembilla
    Division of Experimental Oncology, Multidisciplinary Oncology Center, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Blood 112:652-60. 2008
    ..Our study reports c-Cbl as a critical regulator of the functional responses of memory T cell subsets and identifies for the first time in humans a mechanism controlling the functional heterogeneity of memory CD4 cells...
  46. ncbi request reprint Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
    Arnaud Didierlaurent
    Swiss Institute for Experimental Cancer Research ISREC, Institute of Biochemistry, University of Lausanne, CH 1066 Epalinges, Switzerland
    Vaccine 22:3395-403. 2004
    ..In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules...
  47. doi request reprint Independent evolution of hypervariable regions of HIV-1 gp120: V4 as a swarm of N-Linked glycosylation variants
    Erika Castro
    Laboratory of AIDS Immunopathogenesis, CHUV, Lausanne, Switzerland
    AIDS Res Hum Retroviruses 24:106-13. 2008
    ....
  48. ncbi request reprint Severe post-EBV encephalopathy associated with myelin oligodendrocyte glycoprotein-specific immune response
    Samantha Jilek
    Divisions of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon, 1011 Lausanne, Switzerland
    J Neuroimmunol 192:192-7. 2007
    ..In conclusion, this detailed study shows that acute EBV infection can trigger a potent auto-inflammatory response in the CNS, without evidence of an overt infection...
  49. pmc Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses
    Melissa L Precopio
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:1405-16. 2007
    ..This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated...
  50. ncbi request reprint Understanding what makes a good versus a bad vaccine
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Eur J Immunol 35:2528-31. 2005
    ..Furthermore, the mouse model of Estcourt and collaborators may represent the basis for the development of powerful in vivo experimental strategies to evaluate vaccine candidates...
  51. pmc Exhaustion of bacteria-specific CD4 T cells and microbial translocation in common variable immunodeficiency disorders
    Matthieu Perreau
    Service of Immunology and Allergy and Service of Infectious Diseases, Department of Medicine, Lausanne University Hospital, University of Lausanne, 1011 Lausanne, Switzerland
    J Exp Med 211:2033-45. 2014
    ....
  52. ncbi request reprint Natalizumab treatment alters the expression of T-cell trafficking marker LFA-1 α-chain (CD11a) in MS patients
    Samantha Jilek
    Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Mult Scler . 2013
    ..To determine the long-term effect of natalizumab (NTZ) treatment on the expression of integrins and chemokine receptors involved in the migration of T cells towards the central nervous system (CNS)...
  53. doi request reprint MOBP-specific cellular immune responses are weaker than MOG-specific cellular immune responses in patients with multiple sclerosis and healthy subjects
    Samantha Jilek
    Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon, 1011 Lausanne, Switzerland
    Neurol Sci 34:539-43. 2013
    ..However, both MS patients and control study subjects had similar myelin-specific T-cell responses at the periphery, thus calling for more precise studies at CNS level...
  54. pmc The number of Toll-like receptor 9-agonist motifs in the adenovirus genome correlates with induction of dendritic cell maturation by adenovirus immune complexes
    Matthieu Perreau
    Division of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland
    J Virol 86:6279-85. 2012
    ..In addition, we showed that antihexon but not antifiber antibodies are responsible for the induction of Ad IC-mediated DC maturation...
  55. pmc Immunization with HIV Gag targeted to dendritic cells followed by recombinant New York vaccinia virus induces robust T-cell immunity in nonhuman primates
    Barbara J Flynn
    Vaccine Research Center and Cellular Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:7131-6. 2011
    ..These data reveal qualitative differences in antibody and T-cell responses to DEC-HIV Gag p24 and Gag p24 protein and show that prime boost with protein and adjuvant followed by NYVAC elicits potent cellular immunity...
  56. ncbi request reprint Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease
    Alexandre Harari
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Nat Med 17:372-6. 2011
    ..4%. Therefore, the proportion of single-positive TNF-α Mtb-specific CD4+ T cells is a new tool for the rapid diagnosis of active tuberculosis disease...
  57. doi request reprint Vitamin D has a direct immunomodulatory effect on CD8+ T cells of patients with early multiple sclerosis and healthy control subjects
    Andreas P Lysandropoulos
    Service of Neurology, Department of Clinical Neurosciences, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Neuroimmunol 233:240-4. 2011
    ..CD4+ T cell depletion or even culture with CD8+ T cells only did not abolish the immunomodulatory effect of 1,25(OH)(2)D(3) on CD8+ T cells, suggesting that 1,25(OH)(2)D(3) can act directly on CD8+ T cells...
  58. ncbi request reprint HIV-1-specific immune response
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland
    Adv Pharmacol 56:75-92. 2008
  59. doi request reprint Low levels of human leukocyte antigen donor-specific antibodies detected by solid phase assay before transplantation are frequently clinically irrelevant
    Vincent Aubert
    Service of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Hum Immunol 70:580-3. 2009
    ..The only AHR episode occurred in a recipient with pretransplantation DSA. More work should be performed to better delineate the precise clinical significance of detecting low titers of DSA before transplantation...
  60. ncbi request reprint Effects of immunosuppressive drugs on HIV infection: implications for solid-organ transplantation
    Donatella Ciuffreda
    Division of Immunology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Transpl Int 20:649-58. 2007
    ..We then discuss the most recent results of solid-organ transplantation in HIV-infected patients...
  61. doi request reprint Long sequence duplications, repeats, and palindromes in HIV-1 gp120: length variation in V4 as the product of misalignment mechanism
    Silvia Guglietta
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, CHUV, Hôpital Beaumont, 1011 Lausanne, Switzerland
    Virology 399:167-75. 2010
    ..It also implies that occurrence of large indels in gp120 is not random but is directed by the presence and distribution of elements of misalignment in the HIV genome...
  62. doi request reprint Large TCR diversity of virus-specific CD8 T cells provides the mechanistic basis for massive TCR renewal after antigen exposure
    Isabelle Miconnet
    Division of Immunology and Allergy, Department of Medicine, University Hospital Center, University of Lausanne, 1011 Lausanne, Switzerland
    J Immunol 186:7039-49. 2011
    ....
  63. ncbi request reprint Functional signatures in antiviral T-cell immunity for monitoring virus-associated diseases
    Giuseppe Pantaleo
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
    Nat Rev Immunol 6:417-23. 2006
    ....
  64. doi request reprint IL-2- and CD25-dependent immunoregulatory mechanisms in the homeostasis of T-cell subsets
    Sven Létourneau
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Allergy Clin Immunol 123:758-62. 2009
    ..This review explores these findings and integrates them into our current understanding of T-cell homeostasis...
  65. pmc Carriers of the fragile X mental retardation 1 (FMR1) premutation allele present with increased levels of cytokine IL-10
    Diana Marek
    Department of Medical Genetics, University of Lausanne, Rue du Bugnon 27, Lausanne, 1005, Switzerland
    J Neuroinflammation 9:238. 2012
    ..Abnormal immunological patterns are often associated with neurodegenerative disorders and implicated in their etiology. We therefore investigated the immune status of FXTAS patients, which had not been assessed prior to this study...
  66. pmc Functional avidity: a measure to predict the efficacy of effector T cells?
    Selena Vigano
    Divisions of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland
    Clin Dev Immunol 2012:153863. 2012
    ..Enhancing our knowledge in this field is relevant for tumor immunotherapy and vaccines design...
  67. ncbi request reprint CSF enrichment of highly differentiated CD8+ T cells in early multiple sclerosis
    Samantha Jilek
    Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Clin Immunol 123:105-13. 2007
    ..024) and than in CD8+ T(HD) cells in PP-MS/OND patients (p=0.006). These data suggest that CD8+ T(HD) cells play a role in the early stages of RR-MS...
  68. pmc Innate immune sensing of modified vaccinia virus Ankara (MVA) is mediated by TLR2-TLR6, MDA-5 and the NALP3 inflammasome
    Julie Delaloye
    Department of Medicine, Infectious Diseases Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    PLoS Pathog 5:e1000480. 2009
    ..Delineation of the host response induced by MVA is critical for improving our understanding of poxvirus antiviral escape mechanisms and for designing new MVA vaccine vectors with improved immunogenicity...
  69. pmc Transepithelial transport of HIV-1 by M cells is receptor-mediated
    Grigorios Fotopoulos
    Swiss Institute for Experimental Cancer Research and Institute of Biochemistry, University of Lausanne, CH 1066 Epalinges, Switzerland
    Proc Natl Acad Sci U S A 99:9410-4. 2002
    ..The follicle-associated epithelium covering human gut lymphoid follicles expresses CCR5, but not CXCR4, and lactosyl cerebroside, suggesting that HIV-1 infection may occur through M cells and enterocytes at these sites...
  70. pmc Treatment of primary HIV-1 infection with cyclosporin A coupled with highly active antiretroviral therapy
    G Paolo Rizzardi
    Laboratory of AIDS Immunopathogenesis, Division of Immunology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Clin Invest 109:681-8. 2002
    ..Appropriate, immune-based therapeutic interventions may represent a valuable complement to HAART for treating HIV infection...
  71. ncbi request reprint HIV-1 T-cell vaccines: evaluating the next step
    Giuseppe Pantaleo
    Lancet Infect Dis 8:82-3. 2008
  72. ncbi request reprint EV02: a Phase I trial to compare the safety and immunogenicity of HIV DNA-C prime-NYVAC-C boost to NYVAC-C alone
    Sheena McCormack
    MRC Clinical Trials Unit, 222 Euston Road, London NW1 2DA, UK
    Vaccine 26:3162-74. 2008
    ..Of the 9 individuals with responses to envelope and other peptides, 8 were in the DNA-C group. These promising results suggest that DNA-C was an effective priming agent, that merits further investigation...
  73. ncbi request reprint Medicine. The need for a global HIV vaccine enterprise
    Richard D Klausner
    Bill and Melinda Gates Foundation, Seattle, WA 98102, USA
    Science 300:2036-9. 2003
    ....
  74. ncbi request reprint Effects of CCR5-Delta32 and CCR2-64I alleles on HIV-1 disease progression: the protection varies with duration of infection
    Stephanie A Mulherin
    Biostatistics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH DHHS, 6120 Executive Boulevard, Rockville, MD 20852, USA
    AIDS 17:377-87. 2003
    ..To examine temporal variation in the effects of CCR5-Delta32 and CCR2-64I chemokine receptor gene polymorphisms on HIV-1 disease progression...
  75. ncbi request reprint Response to antiretroviral treatment in HIV-1-infected individuals with allelic variants of the multidrug resistance transporter 1: a pharmacogenetics study
    Jacques Fellay
    Division of Infectious Diseases, University Hospital of Lausanne, Lausanne, Switzerland
    Lancet 359:30-6. 2002
    ..This variability could have a genetic basis. We did a pharmacogenetics study to analyse the association between response to antiretroviral treatment and allelic variants of several genes...
  76. ncbi request reprint Long-term persistence of HIV-1 vaccine-induced CD4+CD45RA-CD62L-CCR7- memory T-helper cells
    Sunita S Balla-Jhagjhoorsingh
    Department of Virology, Biomedical Primate Research Centre, 2280 GH Rijswijk, The Netherlands
    AIDS 18:837-48. 2004
    ..To determine in chimpanzees if candidate HIV-1 subunit protein vaccines were capable of eliciting long-lasting T-cell memory responses in the absence of viral infection, and to determine the specific characteristics of these responses...
  77. ncbi request reprint Long-term efficacy and safety of atazanavir with stavudine and lamivudine in patients previously treated with nelfinavir or atazanavir
    Robin Wood
    Somerset Hospital, Capetown, South Africa and Chulalongkorn University, Bangkok, Thailand
    J Acquir Immune Defic Syndr 36:684-92. 2004
    ..In virologically suppressed nelfinavir-treated patients switched to atazanavir, virologic improvement continued, whereas nelfinavir-induced lipid elevations were reversed within 12 weeks, approaching pretreatment values...
  78. ncbi request reprint Loss of HIV-1-specific T cell proliferation in chronic HIV-1 infection: cause or consequence of viral replication?
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    AIDS 19:1225-7. 2005
  79. ncbi request reprint Three-year immune reconstitution in PI-sparing and PI-containing antiretroviral regimens in advanced HIV-1 disease
    Assia Samri
    Laboratoire d Immunologie Cellulaire, AP HP, Hopital Pitie Salpetriere, Paris, France
    Antivir Ther 12:553-8. 2007
    ..The long-term immunological benefit of protease inhibitor (PI)-sparing antiretroviral therapy (ART) using non-nucleoside reverse transcriptase inhibitors (NNRTIs) remains poorly investigated...
  80. ncbi request reprint Immune-based interventions in HIV infection: doing the right studies, getting the right answers
    Pierre Alexandre Bart
    AIDS 20:617-8. 2006
  81. ncbi request reprint Head-to-head comparison on the immunogenicity of two HIV/AIDS vaccine candidates based on the attenuated poxvirus strains MVA and NYVAC co-expressing in a single locus the HIV-1BX08 gp120 and HIV-1(IIIB) Gag-Pol-Nef proteins of clade B
    Carmen Elena Gómez
    Department of Molecular and Cellular Biology, Centro Nacional de Biotecnologia, CSIC, Ciudad Universitaria Cantoblanco, 28049 Madrid, Spain
    Vaccine 25:2863-85. 2007
    ..All of the immunization protocols induced some humoral response against the gp160 protein from HIV-1 clone LAV. Our findings indicate that MVA-B and NYVAC-B meet the criteria to be potentially useful vaccine candidates against HIV/AIDS...
  82. ncbi request reprint Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade C
    Carmen Elena Gómez
    Department of Molecular and Cellular Biology, Centro Nacional de Biotecnologia, CSIC, Ciudad Universitaria Cantoblanco, 28049 Madrid, Spain
    Vaccine 25:1969-92. 2007
    ..These results demonstrate the immunogenic potential of MVA-C and NYVAC-C as novel vaccine candidates against clade C of HIV-1...
  83. ncbi request reprint Studies of a prophylactic HIV-1 vaccine candidate based on modified vaccinia virus Ankara (MVA) with and without DNA priming: effects of dosage and route on safety and immunogenicity
    Barry S Peters
    Kings College London, UK
    Vaccine 25:2120-7. 2007
    ..Modified vaccinia virus Ankara (MVA) and plasmid DNA (pTHr) expressed HIV-1 clade A gag p24 and p17 fused to a string of 25 overlapping CD8+ T cell epitopes (HIVA)...

Research Grants2

  1. South-West European HIV Vaccine Clinical Trial Unit
    Giuseppe Pantaleo; Fiscal Year: 2007
    ..administrative component: ..
  2. South-West European HIV Vaccine Clinical Trial Unit
    Giuseppe Pantaleo; Fiscal Year: 2008
    ..administrative component: ..