D Moradpour

Summary

Affiliation: Centre Hospitalier Universitaire Vaudois
Country: Switzerland

Publications

  1. pmc Membrane association of the RNA-dependent RNA polymerase is essential for hepatitis C virus RNA replication
    Darius Moradpour
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 44, CH 1011 Lausanne, Switzerland
    J Virol 78:13278-84. 2004
  2. ncbi request reprint [Diagnosis of hepatitis B and C]
    D Moradpour
    Abteilung Innere Medizin II, Medizinische Universitätsklinik Freiburg i Br
    Praxis (Bern 1994) 94:66-72. 2005
  3. ncbi request reprint Pathogenesis of hepatocellular carcinoma
    Darius Moradpour
    Department of Medicine II, University of Freiburg, Germany
    Eur J Gastroenterol Hepatol 17:477-83. 2005
  4. ncbi request reprint The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein
    T Hugle
    Department of Medicine II, University of Freiburg, Freiburg, D 79106, Germany
    Virology 284:70-81. 2001
  5. pmc Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines
    B Wolk
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Virol 74:2293-304. 2000
  6. ncbi request reprint [Hepatitis C]
    D Moradpour
    Abteilung Innere Medizin II, Medizinische Universitätsklinik Freiburg i Br
    Ther Umsch 61:493-8. 2004
  7. ncbi request reprint Current and evolving therapies for hepatitis C
    D Moradpour
    Department of Medicine II, University of Freiburg, Germany
    Eur J Gastroenterol Hepatol 11:1199-202. 1999
  8. ncbi request reprint Hepatitis C virus infection: in vivo and in vitro models
    V Brass
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    J Viral Hepat 14:64-7. 2007
  9. ncbi request reprint Hepatitis C: an update
    D Moradpour
    Department of Medicine II, University of Freiburg, Freiburg i. Br, Germany
    Swiss Med Wkly 131:291-8. 2001
  10. doi request reprint Nonstructural protein 3-4A: the Swiss army knife of hepatitis C virus
    K Morikawa
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Viral Hepat 18:305-15. 2011

Collaborators

Detail Information

Publications56

  1. pmc Membrane association of the RNA-dependent RNA polymerase is essential for hepatitis C virus RNA replication
    Darius Moradpour
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 44, CH 1011 Lausanne, Switzerland
    J Virol 78:13278-84. 2004
    ..These results have implications for the functional architecture of the HCV replication complex and provide new insights into the expanding spectrum of tail-anchored proteins...
  2. ncbi request reprint [Diagnosis of hepatitis B and C]
    D Moradpour
    Abteilung Innere Medizin II, Medizinische Universitätsklinik Freiburg i Br
    Praxis (Bern 1994) 94:66-72. 2005
    ..Molecular assays for the detection of HBV DNA or HCV RNA are in some instances needed to confirm the diagnosis. However, their principal role is in the therapeutic decision making and in the monitoring of antiviral therapy...
  3. ncbi request reprint Pathogenesis of hepatocellular carcinoma
    Darius Moradpour
    Department of Medicine II, University of Freiburg, Germany
    Eur J Gastroenterol Hepatol 17:477-83. 2005
    ..New technologies, including gene expression profiling and proteomic analyses, should allow us to further elucidate the molecular events underlying HCC development and identify novel diagnostic markers as well as therapeutic targets...
  4. ncbi request reprint The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein
    T Hugle
    Department of Medicine II, University of Freiburg, Freiburg, D 79106, Germany
    Virology 284:70-81. 2001
    ..Taken together, our findings suggest that NS4B is a component of a membrane-associated cytoplasmic HCV replication complex. An efficient replication system will be essential to further define the role of NS4B in the viral life cycle...
  5. pmc Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines
    B Wolk
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Virol 74:2293-304. 2000
    ....
  6. ncbi request reprint [Hepatitis C]
    D Moradpour
    Abteilung Innere Medizin II, Medizinische Universitätsklinik Freiburg i Br
    Ther Umsch 61:493-8. 2004
    ..Recent progress has allowed the identification of novel antiviral targets and therapeutic strategies. These will likely complement existing therapeutic modalities in the near future...
  7. ncbi request reprint Current and evolving therapies for hepatitis C
    D Moradpour
    Department of Medicine II, University of Freiburg, Germany
    Eur J Gastroenterol Hepatol 11:1199-202. 1999
    ..These and other novel antiviral strategies may complement the existing therapeutic modalities in the future...
  8. ncbi request reprint Hepatitis C virus infection: in vivo and in vitro models
    V Brass
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    J Viral Hepat 14:64-7. 2007
    ..In the following we will review the different in vivo and in vitro models of HCV infection...
  9. ncbi request reprint Hepatitis C: an update
    D Moradpour
    Department of Medicine II, University of Freiburg, Freiburg i. Br, Germany
    Swiss Med Wkly 131:291-8. 2001
    ..Here, we briefly review current concepts of the epidemiology, molecular virology, pathogenesis, natural history, diagnosis, therapy, and prevention of hepatitis C...
  10. doi request reprint Nonstructural protein 3-4A: the Swiss army knife of hepatitis C virus
    K Morikawa
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    J Viral Hepat 18:305-15. 2011
    ..This review provides a concise update on the biochemical and structural aspects of NS3-4A, its role in the pathogenesis of chronic hepatitis C and the clinical development of NS3-4A protease inhibitors...
  11. ncbi request reprint Expression of hepatitis C virus proteins does not interfere with major histocompatibility complex class I processing and presentation in vitro
    D Moradpour
    Department of Medicine II, University of Freiburg, Germany
    Hepatology 33:1282-7. 2001
    ..Other mechanisms, such as interference with the interferon system, may be operative in HCV infection, leading to viral persistence...
  12. ncbi request reprint Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase
    J Schmidt-Mende
    Department of Medicine II, University of Freiburg, D-79106 Freiburg, Germany
    J Biol Chem 276:44052-63. 2001
    ..Formation of the HCV replication complex, therefore, involves specific determinants for membrane association that represent potential targets for antiviral intervention...
  13. ncbi request reprint [Molecular virology of hepatitis C]
    D Moradpour
    Abteilung Innere Medizin II, Medizinische Universitätsklinik Freiburg i Br
    Praxis (Bern 1994) 91:2247-53. 2002
    ..In vitro and in vivo models of HCV replication will be discussed in this context. Finally, novel antiviral strategies will be outlined that result from an improved understanding of the viral life cycle...
  14. ncbi request reprint Hepatitis C: a concise review
    D Moradpour
    Department of Medicine II, University of Freiburg, Germany
    Minerva Med 92:329-39. 2001
    ..Here, we briefly review current concepts of the epidemiology, molecular virology, pathogenesis, natural history, diagnosis, therapy, and prevention of hepatitis C...
  15. ncbi request reprint [When and how to treat hepatitis B and C?]
    I Pache
    Toronto Western Hospital, Liver Clinic, 399 Bathurst St, Toronto, Ontario, M5ST 2S8, Canada
    Rev Med Suisse 1:237-41. 2005
    ..Novel antiviral strategies are currently being explored...
  16. ncbi request reprint [Complications of liver cirrhosis: oesophageal varices, ascites and hepato-cellular carcinoma]
    F X Troillet
    Service de Chirurgie Viscerale, CHUV, Lausanne
    Rev Med Suisse 1:249-50, 252-5. 2005
    ..Surgery is only possible in a small number of cases. Percutaneous destruction techniques have nearly the same survival rate as that obtained by surgery and should be proposed to patients where surgery is not an option...
  17. ncbi request reprint [Therapy of hepatitis C]
    D Moradpour
    Abteilung Innere Medizin II, Medizinische Universitätsklinik Freiburg i Br
    Praxis (Bern 1994) 91:977-82. 2002
    ..These will likely complement existing therapeutic modalities in the near future...
  18. ncbi request reprint Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins
    Benno Wölk
    Department of Medicine II, University of Freiburg, Hugstetter Str 55, D 79106 Freiburg, Germany
    J Gen Virol 86:1737-46. 2005
    ..In conclusion, these cell lines represent a unique tool to study the cellular immune response, as well as to evaluate novel vaccine and immunotherapeutic strategies against HCV...
  19. ncbi request reprint [Highlights in hepatology]
    Isabelle Pache
    Service de gastro entérologie et d hépatologie, CHUV, 1011 Lausanne
    Rev Med Suisse 4:214, 216, 218-20. 2008
    ..Finally, we will briefly comment new findings in alcoholic hepatitis as well as acetaminophen hepatotoxicity and summarize revised criteria for the diagnosis and management of hepatorenal syndrome...
  20. ncbi request reprint A primer on the molecular virology of hepatitis C
    Darius Moradpour
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    Liver Int 24:519-25. 2004
    ..Here, we provide a primer on the molecular virology of hepatitis C, with particular reference to novel antiviral targets and therapeutic strategies...
  21. ncbi request reprint Hepatitis C virus comes full circle: production of recombinant infectious virus in tissue culture
    Jan Martin Berke
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland
    Hepatology 42:1264-9. 2005
  22. ncbi request reprint Function follows form: the structure of the N-terminal domain of HCV NS5A
    Darius Moradpour
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Hepatology 42:732-5. 2005
    ..5-A resolution, which contains a novel fold, a new zinc-coordination motif, and a disulfide bond. We use molecular surface analysis to suggest the location of protein-, RNA-, and membrane-interaction sites...
  23. ncbi request reprint Replication of hepatitis C virus
    Darius Moradpour
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH 1011 Lausanne, Switzerland
    Nat Rev Microbiol 5:453-63. 2007
    ..These efforts have already translated into the identification of novel antiviral targets and the development of new therapeutic strategies, some of which are currently undergoing clinical evaluation...
  24. pmc A dynamic view of hepatitis C virus replication complexes
    Benno Wölk
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    J Virol 82:10519-31. 2008
    ..We suggest that this MT-dependent transport sustains two distinct RC populations, which are both required during the HCV life cycle...
  25. ncbi request reprint [Hepatocellular carcinoma: a multidisciplinary approach]
    Adrien A Tempia-Caliera
    Service de Chirurgie Viscerale, CHUV, 1011 Lausanne
    Rev Med Suisse 1:1621-4, 1627-9. 2005
    ..Follow-up is crucial as recurrences can be treated by following similar algorithms. The efficacy of oncological adjuvant and neoadjuvant treatment is not yet proved...
  26. pmc Characterization of nonstructural protein membrane anchor deletion mutants expressed in the context of the hepatitis C virus polyprotein
    Rainer Gosert
    Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 46, CH 1011 Lausanne, Switzerland
    J Virol 79:7911-7. 2005
    ..This feature may provide the nonstructural proteins with a certain flexibility to perform their multiple functions during HCV replication...
  27. ncbi request reprint Of mice and men: a small animal model of hepatitis C virus replication
    Volker Brass
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    Hepatology 35:722-4. 2002
  28. pmc The hepatitis C virus RNA-dependent RNA polymerase membrane insertion sequence is a transmembrane segment
    Natalia Ivashkina
    Department of Medicine II, University Hospital, University of Freiburg, Hugstetter Strasse 55, D 79106 Freiburg, Germany
    J Virol 76:13088-93. 2002
    ..These findings have important implications for the functional architecture of the HCV replication complex...
  29. ncbi request reprint Viral pathogenesis of hepatocellular carcinoma
    Hubert E Blum
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    J Gastroenterol Hepatol 17:S413-20. 2002
  30. pmc Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons
    Rainer Gosert
    Department of Medicine II, University of Freiburg, Germany
    J Virol 77:5487-92. 2003
    ..Metabolic labeling with 5-bromouridine 5'-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV...
  31. ncbi request reprint Expression of hepatitis c virus proteins inhibits interferon alpha signaling in the liver of transgenic mice
    Alex Blindenbacher
    Department of Research, University Hospital Basel, Switzerland
    Gastroenterology 124:1465-75. 2003
    ..Interference of viral proteins with the Jak-STAT pathway could be responsible for interferon resistance in patients with chronic HCV...
  32. ncbi request reprint Membrane association of hepatitis C virus nonstructural proteins and identification of the membrane alteration that harbors the viral replication complex
    Darius Moradpour
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    Antiviral Res 60:103-9. 2003
    ..These findings add to our current understanding of the HCV life cycle and may ultimately allow to design novel antiviral strategies against hepatitis C...
  33. pmc Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex
    Denise Egger
    Institute for Medical Microbiology, University of Basel, CH 4003 Basel, Switzerland
    J Virol 76:5974-84. 2002
    ..Virchows Arch. B., 33:233-243, 1980). In view of this finding and the observation that all HCV proteins accumulate on the membranous web, we propose that the membranous web forms the viral replication complex in HCV-infected cells...
  34. pmc Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes
    Darius Moradpour
    Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10021, USA
    J Virol 78:7400-9. 2004
    ..These findings reveal an unexpected flexibility of the C-terminal domain of NS5A and provide tools for studying the formation and turnover of HCV replication complexes in living cells...
  35. ncbi request reprint [Management of chronic viral hepatitis]
    Isabelle Pache
    Service de gastro entérologie et d hépatologie, CHUV, 1011 Lausanne
    Rev Med Suisse 3:215, 217-8, 221-3. 2007
    ..Finally, recent studies using pegylated interferon-alpha have shown promising results for the treatment of chronic hepatitis D...
  36. ncbi request reprint A new twist to translation initiation of the hepatitis C virus
    Rainer Gosert
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    Hepatology 35:724-6. 2002
  37. ncbi request reprint Combined antiviral therapy for recurrent hepatitis C virus after liver transplantation
    Massimiliano Fontana
    Transplantation 83:525-6. 2007
  38. ncbi request reprint Recent developments in target identification against hepatitis C virus
    Volker Brass
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    Expert Opin Ther Targets 8:295-307. 2004
    ..These and other novel antiviral strategies may complement existing therapeutic modalities in the future...
  39. ncbi request reprint Hepatitis C virus infection in haemodialysis and kidney transplant patients
    Seema Baid-Agrawal
    Department of Nephrology and Medical Intensive Care, Campus Virchow Klinikum, Charite Universitatsmedizin Berlin, Berlin, Germany
    Rev Med Virol 18:97-115. 2008
    ..Large multi-centre clinical trials are required in HCV-infected haemodialysis and kidney transplant patients in order to define optimal therapeutic strategies before and after transplantation...
  40. ncbi request reprint An amino-terminal amphipathic alpha-helix mediates membrane association of the hepatitis C virus nonstructural protein 5A
    Volker Brass
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Biol Chem 277:8130-9. 2002
    ..These findings have important implications for the structural and functional organization of the HCV replication complex and may define novel targets for antiviral intervention...
  41. ncbi request reprint [Liver transplantation: new organ allocation system in Switzerland]
    Massimiliano Fontana
    Centre de transplantation d organes, CHUV, 1011 Lausanne
    Rev Med Suisse 4:221-3. 2008
    ..Advantages and limitations of the MELD score are discussed. Finally the West Switzerland joint liver transplantation program is briefly introduced...
  42. ncbi request reprint Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase
    Darius Moradpour
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Biol Chem 277:593-601. 2002
    ..More important, detailed knowledge of the mAb 5B-12B7 contact sites on the enzyme may facilitate the development of small molecule RdRp inhibitors as novel antiviral agents...
  43. pmc Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex
    Volker Brass
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    Proc Natl Acad Sci U S A 105:14545-50. 2008
    ..This model has implications for the functional architecture of the HCV replication complex, proteolytic targeting of host factors, and drug design...
  44. pmc Conserved determinants for membrane association of nonstructural protein 5A from hepatitis C virus and related viruses
    Volker Brass
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Virol 81:2745-57. 2007
    ..This observation points to conserved roles of the N-terminal amphipathic alpha helices of NS5A in replication complex formation...
  45. pmc euHCVdb: the European hepatitis C virus database
    Christophe Combet
    Institut de Biologie et Chimie des Protéines UMR5086, CNRS, University of Lyon 1, IFR 128 BioSciences, Lyon Gerland, France
    Nucleic Acids Res 35:D363-6. 2007
    ..As part of an international collaborative effort with the US and Japanese databases, the European HCV Database (euHCVdb) is mainly dedicated to HCV protein sequences, 3D structures and functional analyses...
  46. ncbi request reprint Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A
    Francois Penin
    Institut de Biologie et Chimie des Proteines, CNRS UMR 5086, IFR128 BioSciences, Lyon Gerland, Lyon F 69367, Cedex 07, France
    J Biol Chem 279:40835-43. 2004
    ....
  47. ncbi request reprint Structural biology of hepatitis C virus
    Francois Penin
    Institut de Biologie et Chimie des Proteines, Lyon, France
    Hepatology 39:5-19. 2004
    ..This article reviews the current knowledge of HCV structural biology...
  48. pmc Cytotoxic T lymphocytes derived from patients with chronic hepatitis C virus infection kill bystander cells via Fas-FasL interaction
    Christel Gremion
    Clinic for Rheumatology and Clinical Immunology Allergology, University of Bern, CH 3010 Bern, Switzerland
    J Virol 78:2152-7. 2004
    ..Our data underscore the role of the Fas pathway in HCV-related liver injury and support the exploration of Fas-based treatment strategies for patients with chronic hepatitis C virus infection...
  49. ncbi request reprint A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognise human cells encoding hepatitis C virus (HCV) proteins
    Olivier B Engler
    Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, Bern, Switzerland
    Vaccine 23:58-68. 2004
    ..In conclusion, such liposome formulations represent a safe and promising strategy to stimulate the CD8(+) T cell against HCV...
  50. ncbi request reprint Antiviral treatment of patients with HBV-related cirrhosis
    Hubert E Blum
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    J Gastroenterol Hepatol 17:S306-10. 2002
  51. ncbi request reprint Interaction of hepatitis C virus proteins with host cell membranes and lipids
    Jean Dubuisson
    CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    Trends Cell Biol 12:517-23. 2002
    ....
  52. ncbi request reprint Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
    ..Cardif thus functions as an adaptor, linking the cytoplasmic dsRNA receptor RIG-I to the initiation of antiviral programmes...
  53. pmc Upregulation of protein phosphatase 2Ac by hepatitis C virus modulates NS3 helicase activity through inhibition of protein arginine methyltransferase 1
    Francois H T Duong
    Department of Research and Division of Gastroenterology and Hepatology, University Hospital Basel, CH 4031 Basel, Switzerland
    J Virol 79:15342-50. 2005
    ..We conclude that up-regulation of PP2A might be crucial for the efficient replication of HCV and propose PP2A as a potential target for anti-HCV treatment strategies...
  54. ncbi request reprint Hepatitis C: molecular virology and antiviral targets
    Darius Moradpour
    Department of Medicine II, University of Freiburg, Hugstetter Strasse 55, Germany
    Trends Mol Med 8:476-82. 2002
    ..Moreover, in vitro and in vivo model systems have been developed that allow systematic evaluation of new therapeutic strategies. This review details current concepts in molecular virology and emerging therapies for hepatitis C...
  55. ncbi request reprint NMR structure and molecular dynamics of the in-plane membrane anchor of nonstructural protein 5A from bovine viral diarrhea virus
    Nicolas Sapay
    Institut de Biologie et Chimie des Proteines, CNRS UMR 5086, IFR128 Biosciences Lyon Gerland, University of Lyon, France
    Biochemistry 45:2221-33. 2006
    ..These structural conservations point toward conserved roles of the N-terminal in-plane membrane anchors of NS5A in replication complex formation of HCV, BVDV, and other related viruses...
  56. ncbi request reprint Hepatitis C virus protein expression causes calcium-mediated mitochondrial bioenergetic dysfunction and nitro-oxidative stress
    Claudia Piccoli
    Department of Biomedical Sciences, University of Foggia, Foggia, Italy
    Hepatology 46:58-65. 2007
    ..These observations provide new insights into the pathogenesis of hepatitis C and may offer new opportunities for therapeutic intervention...