Harald Brussow

Summary

Country: Switzerland

Publications

  1. ncbi request reprint Phages of dairy bacteria
    H Brussow
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, Lausanne 26, CH 1000 Switzerland
    Annu Rev Microbiol 55:283-303. 2001
  2. pmc Lactobacillus plantarum bacteriophage LP65: a new member of the SPO1-like genus of the family Myoviridae
    Sandra Chibani-Chennoufi
    Nestle Research Centre, CH 1000 Lausanne, 26 Vers chez les Blanc, Switzerland
    J Bacteriol 186:7069-83. 2004
  3. ncbi request reprint Phage therapy: the Escherichia coli experience
    Harald Brussow
    Nestle Research Centre, CH 1000 Lausanne 26 Vers chez les Blanc, Switzerland
    Microbiology 151:2133-40. 2005
  4. doi request reprint Nutrition, population growth and disease: a short history of lactose
    Harald Brussow
    Nestlé Research Center Lausanne, BioAnalytical Science Department, Food and Health Microbiology, CH 1000, Lausanne, 26, Switzerland
    Environ Microbiol 15:2154-61. 2013
  5. doi request reprint Bacteriophage-host interaction: from splendid isolation into a messy reality
    Harald Brussow
    Nestle Research Centre, BioAnalytical Science Department, Food and Health Microbiology, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    Curr Opin Microbiol 16:500-6. 2013
  6. doi request reprint What is health?
    Harald Brussow
    Nestle Research Center, Bioanalytical Sciences, Food and Health Microbiology, Lausanne, Switzerland
    Microb Biotechnol 6:341-8. 2013
  7. doi request reprint Microbiota and healthy ageing: observational and nutritional intervention studies
    Harald Brussow
    Bioanalytical Sciences, Food and Health Microbiology, Nestle Research Center, Lausanne, Switzerland
    Microb Biotechnol 6:326-34. 2013
  8. doi request reprint What is needed for phage therapy to become a reality in Western medicine?
    Harald Brussow
    Nestle Research Center, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Virology 434:138-42. 2012
  9. pmc Shiga toxin-producing Escherichia coli O104:H4: a new challenge for microbiology
    Maite Muniesa
    Department of Microbiology, University of Barcelona, Barcelona, Spain
    Appl Environ Microbiol 78:4065-73. 2012
  10. pmc Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies
    Neha Pant
    Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    BMC Microbiol 7:86. 2007

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Phages of dairy bacteria
    H Brussow
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, Lausanne 26, CH 1000 Switzerland
    Annu Rev Microbiol 55:283-303. 2001
    ..Virulent lactococcal phages with prolate heads (c2-like genus of Siphoviridae), in contrast, have no known counterparts in other bacterial genera...
  2. pmc Lactobacillus plantarum bacteriophage LP65: a new member of the SPO1-like genus of the family Myoviridae
    Sandra Chibani-Chennoufi
    Nestle Research Centre, CH 1000 Lausanne, 26 Vers chez les Blanc, Switzerland
    J Bacteriol 186:7069-83. 2004
    ..The putative tail sheath protein showed a shear-induced change in electrophoretic migration behavior. The genome organization of the structural module in LP65 resembled that of Siphoviridae from the lambda supergroup...
  3. ncbi request reprint Phage therapy: the Escherichia coli experience
    Harald Brussow
    Nestle Research Centre, CH 1000 Lausanne 26 Vers chez les Blanc, Switzerland
    Microbiology 151:2133-40. 2005
    ..These proposals have a long history, but are currently going through a kind of renaissance as documented by a spate of recent reviews. This review discusses the potential of phage therapy with a specific example, namely Escherichia coli...
  4. doi request reprint Nutrition, population growth and disease: a short history of lactose
    Harald Brussow
    Nestlé Research Center Lausanne, BioAnalytical Science Department, Food and Health Microbiology, CH 1000, Lausanne, 26, Switzerland
    Environ Microbiol 15:2154-61. 2013
    ..Lactase is also temporarily lost in rotavirus and Escherichia coli childhood diarrhoea and persistent diarrhoea is consequently best treated with lactose-free diets. ..
  5. doi request reprint Bacteriophage-host interaction: from splendid isolation into a messy reality
    Harald Brussow
    Nestle Research Centre, BioAnalytical Science Department, Food and Health Microbiology, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    Curr Opin Microbiol 16:500-6. 2013
    ..Phages were already used successfully to reduce contamination levels with medical catheters and might likewise be of use against infections frequently forming bacterial biofilms. ..
  6. doi request reprint What is health?
    Harald Brussow
    Nestle Research Center, Bioanalytical Sciences, Food and Health Microbiology, Lausanne, Switzerland
    Microb Biotechnol 6:341-8. 2013
    ..Since the health impact of the human gut microbiome is currently a topical research area, microbiologists should be aware of the problems in defining health...
  7. doi request reprint Microbiota and healthy ageing: observational and nutritional intervention studies
    Harald Brussow
    Bioanalytical Sciences, Food and Health Microbiology, Nestle Research Center, Lausanne, Switzerland
    Microb Biotechnol 6:326-34. 2013
    ..However, in the ELDERMET study, microbiota changes do not precede, but follow the changes in health status of elderly subjects possibly as a consequence of diet changes...
  8. doi request reprint What is needed for phage therapy to become a reality in Western medicine?
    Harald Brussow
    Nestle Research Center, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Virology 434:138-42. 2012
    ..In view of the growing antibiotic resistance crisis, a coordinated effort by the public health sector is needed to evaluate the potential of phage therapy as an adjunct to antibiotics...
  9. pmc Shiga toxin-producing Escherichia coli O104:H4: a new challenge for microbiology
    Maite Muniesa
    Department of Microbiology, University of Barcelona, Barcelona, Spain
    Appl Environ Microbiol 78:4065-73. 2012
    ..The epidemic O104:H4 strains were resistant to all penicillins and cephalosporins but susceptible to carbapenems, which were recommended for treatment...
  10. pmc Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies
    Neha Pant
    Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    BMC Microbiol 7:86. 2007
    ..Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti - rotavirus antibodies in a mouse model of rotavirus diarrhea...
  11. pmc Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion
    Harald Brussow
    Nestle, Research Center, Vers chez les Blanc, CH 1000 Lausanne, Switzerland
    Microbiol Mol Biol Rev 68:560-602, table of contents. 2004
    ..In addition, they can serve as anchoring points for genome inversions. The current review presents the available genomics and biological data on prophages from bacterial pathogens in an evolutionary framework...
  12. ncbi request reprint Comparative phage genomics and the evolution of Siphoviridae: insights from dairy phages
    H Brussow
    Nestle Research Centre, Nestec Ltd, CH 1000 Lausanne 26, Switzerland
    Mol Microbiol 39:213-22. 2001
    ..We suggest that tailed phages are the result of both vertical and horizontal evolution and are thus a good model system for web-like phylogenies...
  13. ncbi request reprint Phage genomics: small is beautiful
    Harald Brussow
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 26 Lausanne, Switzerland
    Cell 108:13-6. 2002
    ....
  14. ncbi request reprint Transcription analysis of Streptococcus thermophilus phages in the lysogenic state
    Marco Ventura
    Nestle Research Center, Nestec Ltd Vers chez les Blanc, CH Lausanne, 26, Switzerland
    Virology 302:21-32. 2002
    ..thermophilus that had lost most of the prophage DNA while transcribed prophage genes were spared from the deletion process...
  15. ncbi request reprint The prophages of Lactobacillus johnsonii NCC 533: comparative genomics and transcription analysis
    Marco Ventura
    Nestle Research Center, Nestec Ltd, CH 1026 Lausanne, Switzerland
    Virology 320:229-42. 2004
    ..A short, but highly transcribed region was located between the phage lysin and right attachment site; it lacked a protein-encoding function in one prophage...
  16. pmc The role of prophage for genome diversification within a clonal lineage of Lactobacillus johnsonii: characterization of the defective prophage LJ771
    Emmanuel Denou
    Nestle Research Center, Nestec Ltd, P O Box 44, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 190:5806-13. 2008
    ..The two L. johnsonii strains had identical in vitro growth and in vivo gut persistence phenotypes. Also, in an isogenic background, the presence of the prophage resulted in no growth disadvantage...
  17. pmc In vitro and in vivo bacteriolytic activities of Escherichia coli phages: implications for phage therapy
    Sandra Chibani-Chennoufi
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Antimicrob Agents Chemother 48:2558-69. 2004
    ..coli colonies were susceptible to the given phage cocktail. Apparently, the resident E. coli gut flora is physically or physiologically protected against phage infection...
  18. doi request reprint A Mesocosm of Lactobacillus johnsonii, Bifidobacterium longum, and Escherichia coli in the mouse gut
    Emmanuel Denou
    Nutrition and Health Department, Nestle Research Center, Lausanne, Switzerland
    DNA Cell Biol 28:413-22. 2009
    ..The study of such simple interacting bacterial systems might help to derive some basic rules governing microbial ecology within the mammalian gut...
  19. doi request reprint T4 phages against Escherichia coli diarrhea: potential and problems
    Emmanuel Denou
    Nestle Research Center, Nestec Ltd, Lausanne 26, Switzerland
    Virology 388:21-30. 2009
    ..coli strains representing the five main pathotypes isolated from diarrhea patients...
  20. pmc Isolation of Escherichia coli bacteriophages from the stool of pediatric diarrhea patients in Bangladesh
    Sandra Chibani-Chennoufi
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 186:8287-94. 2004
    ..Phages with closely related restriction patterns and, in the case of T4-like phages, identical g23 gene sequences were isolated from different patients, suggesting epidemiological links between the patients...
  21. pmc Integration and distribution of Lactobacillus johnsonii prophages
    Marco Ventura
    Nestle Research Center, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    J Bacteriol 185:4603-8. 2003
    ..In a survey, the prophages were restricted to strains sharing an essentially identical restriction pattern. Microarray analysis showed that the prophage DNA represents about 50% of the NCC533 strain-specific DNA...
  22. pmc Comparative genomics of the T4-Like Escherichia coli phage JS98: implications for the evolution of T4 phages
    Sandra Chibani-Chennoufi
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 186:8276-86. 2004
    ..Comparative genomics of 100-kb genome sequence revealed that T4-like phages diversify more by the accumulation of point mutations and occasional gene duplication events than by modular exchanges...
  23. ncbi request reprint Transcription mapping as a tool in phage genomics: the case of the temperate Streptococcus thermophilus phage Sfi21
    Marco Ventura
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, Lausanne 26, CH 1000, Switzerland
    Virology 296:62-76. 2002
    ..The involvement of phage-encoded regulators for middle and late transcription was suggested by chloramphenicol-inhibition experiments...
  24. doi request reprint In vivo replication of T4 and T7 bacteriophages in germ-free mice colonized with Escherichia coli
    Marietta Weiss
    Nestle Research Center, Nestec Ltd P O Box 44, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Virology 393:16-23. 2009
    ..The difference between T7 and T4 with respect to gut replication might partly reflect their distinct in vitro capacity to replicate on slowly growing cells...
  25. ncbi request reprint Phage as agents of lateral gene transfer
    Carlos Canchaya
    Nestle Research Centre, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    Curr Opin Microbiol 6:417-24. 2003
    ..Occasionally, phages are also involved in the lateral transfer of other mobile DNA elements or bacterial DNA. Recent advances in the field of genomics have revealed a major impact by phages on bacterial chromosome evolution...
  26. pmc Temporal transcription map of the virulent Streptococcus thermophilus bacteriophage Sfi19
    Marco Ventura
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Appl Environ Microbiol 70:5041-6. 2004
    ..Chloramphenicol treatment suppressed the translation of a putative transcriptional factor necessary for the production of late transcripts and shifted middle transcripts to early transcription times...
  27. pmc Gene expression of commensal Lactobacillus johnsonii strain NCC533 during in vitro growth and in the murine gut
    Emmanuel Denou
    Nestle Research Centre, Nestec Ltd, P O Box 44, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 189:8109-19. 2007
    ..Our data revealed not only major differences between in vitro- and in vivo-expressed genes in a Lactobacillus gut commensal organism but also marked changes in the expression of genes along the digestive tract...
  28. ncbi request reprint Genome analysis of an inducible prophage and prophage remnants integrated in the Streptococcus pyogenes strain SF370
    Carlos Canchaya
    Nestle Research Center, Nestec Ltd Vers chez les Blanc, CH Lausanne 26, Switzerland
    Virology 302:245-58. 2002
    ..1 and S. pneumoniae phage MM1 integrated into homologous chromosomal locations. The prophage sequences were interpreted with a hypothesis that predicts elements of cooperation and an arms race between phage and host genomes...
  29. pmc The dilemma of phage taxonomy illustrated by comparative genomics of Sfi21-like Siphoviridae in lactic acid bacteria
    Caroline Proux
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 184:6026-36. 2002
    ..In this system the currently sequenced lactococcal phages would be grouped into five genera: c2-, sk1, Sfi11-, r1t-, and Sfi21-like phages...
  30. pmc Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy
    Anne Bruttin
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Antimicrob Agents Chemother 49:2874-8. 2005
    ..This is, to our knowledge, the first safety test in the recent English literature which has measured the bioavailability of oral phage in humans and is thus a first step to the rational evaluation of phage therapy for diarrheal diseases...
  31. ncbi request reprint The prophage sequences of Lactobacillus plantarum strain WCFS1
    Marco Ventura
    Nestle Research Center, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    Virology 316:245-55. 2003
    ..Both prophages harbored tRNA genes. R-Lp3 and R-Lp4 represent short prophage remnants; R-Lp3 abuts Lp2 and displays sequence links to cos-site Siphoviridae...
  32. pmc Phage-host interaction: an ecological perspective
    Sandra Chibani-Chennoufi
    Nestle Research Centre, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 186:3677-86. 2004
  33. pmc Identification of genes associated with the long-gut-persistence phenotype of the probiotic Lactobacillus johnsonii strain NCC533 using a combination of genomics and transcriptome analysis
    Emmanuel Denou
    Nestle Research Centre, CH 1000 Lausanne 26 Vers chez les Blanc, Switzerland
    J Bacteriol 190:3161-8. 2008
    ..The experiments revealed that deletion of LJ1654 to LJ1656 and LJ1680 decreased the gut residence time, while a mutant with a deleted exopolysaccharide biosynthesis cluster had a slightly increased residence time...
  34. doi request reprint Safety analysis of a Russian phage cocktail: from metagenomic analysis to oral application in healthy human subjects
    Shawna McCallin
    Nestle Research Centre, Nestec Ltd, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Virology 443:187-96. 2013
    ..Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure...
  35. ncbi request reprint The impact of prophages on bacterial chromosomes
    Carlos Canchaya
    Nestle Research Centre, Nutrition and Health Department Functional Microbiology Group, CH 1000 Lausanne 26 Vers chez les Blanc, Switzerland
    Mol Microbiol 53:9-18. 2004
    ..Prophages are apparently as easily acquired as they are lost from the bacterial chromosome. Fixation of prophage genes seems to be restricted to those with functions that have been co-opted by the bacterial host...
  36. pmc Prophage genomics
    Carlos Canchaya
    Nestle Research Center, Vers chez les Blanc, CH 1000 Lausanne 26, Switzerland
    Microbiol Mol Biol Rev 67:238-76, table of contents. 2003
    ..The effect of selection pressure on the host bacterium is apparently an important force shaping the prophage genomes in low-G+C gram-positive bacteria and gamma-proteobacteria...
  37. ncbi request reprint DNA-binding activity of the Streptococcus thermophilus phage Sfi21 repressor
    Anne Bruttin
    Nestle Research Center, Nestec Ltd Vers chez les Blanc, CH Lausanne, 26, Switzerland
    Virology 303:100-9. 2002
    ..However, when increasing amounts of orf 75 gp containing cell extracts were added to orf 127 gp containing cell extracts, the repressor could no longer bind its operator site...
  38. ncbi request reprint Comparative genomics of phages and prophages in lactic acid bacteria
    Frank Desiere
    Nestle Research Center, Nestec Ltd, Vers chez les Blanc, CH Lausanne 26, Switzerland
    Antonie Van Leeuwenhoek 82:73-91. 2002
    ..These genes belong to the few prophage genes transcribed in the lysogen. Prophages from dairy bacteria might therefore also contribute to the evolutionary fitness of non-pathogenic LAB...
  39. doi request reprint Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails
    Gilles Bourdin
    Nutrition and Health Department, Nestle Research Center, Lausanne, Switzerland
    Microb Biotechnol 7:165-76. 2014
    ..Since the coverage of a given T4-like phage cocktail differed with geographical area and epidemiological setting, a phage composition adapted to a local situation is needed for phage therapy approaches against E. coli pathogens. ..
  40. doi request reprint Amplification and purification of T4-like escherichia coli phages for phage therapy: from laboratory to pilot scale
    Gilles Bourdin
    Nestle Research Centre, Nestec Ltd, Vers chez les Blanc, Lausanne, Switzerland
    Appl Environ Microbiol 80:1469-76. 2014
    ..T4-like phages could not be pasteurized but maintained their infectivity titer in the cold chain. In the presence of 10 mM magnesium ions, phages showed no loss of titer over 1 month at 30°C. ..
  41. pmc Genome analysis of phage JS98 defines a fourth major subgroup of T4-like phages in Escherichia coli
    Sophie Zuber
    Nestle Research Center, Nestec Ltd, P O Box 44, CH 1000 Lausanne 26, Switzerland
    J Bacteriol 189:8206-14. 2007
    ..No evidence for DNA exchange between JS98 phage and the E. coli host genome or coliphages other than T4 was observed. No undesired genes which could compromise its medical use were detected in the JS98 genome sequence...
  42. pmc Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics
    Bernard Berger
    Nestle Research Center, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    J Bacteriol 189:1311-21. 2007
    ..Modern whole-genome-based techniques are thus a useful adjunct to the clarification of taxonomical relationships in problematic bacterial groups...
  43. doi request reprint Decreasing Enterobacter sakazakii (Cronobacter spp.) food contamination level with bacteriophages: prospects and problems
    Sophie Zuber
    Nestle Research Center, Nestec Ltd, Lausanne, Switzerland
    Microb Biotechnol 1:532-43. 2008
    ..Addition of trehalose or milk formula stabilized the phage preparation, which then showed excellent storage stability even at elevated temperature...