Martin J Stoddart

Summary

Affiliation: AO Research Institute
Country: Switzerland

Publications

  1. doi request reprint WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
    Martin J Stoddart
    AO Research Institute Davos, Davos Platz, Switzerland
    Methods Mol Biol 740:21-5. 2011
  2. doi request reprint Cells and secretome--towards endogenous cell re-activation for cartilage repair
    Martin J Stoddart
    AO Research Institute Davos, 7270 Davos Platz, Switzerland Electronic address
    Adv Drug Deliv Rev 84:135-45. 2015
  3. doi request reprint Cells and biomaterials in cartilage tissue engineering
    Martin J Stoddart
    Biomaterials and Tissue Engineering, AO Research Institute, Davos Platz, Switzerland
    Regen Med 4:81-98. 2009
  4. doi request reprint Cell viability assays: introduction
    Martin J Stoddart
    AO Research Institute Davos, Davos Platz, Switzerland
    Methods Mol Biol 740:1-6. 2011
  5. pmc A rapid method for the generation of uniform acellular bone explants: a technical note
    Katharina Jahn
    AO Research Institute, Clavadelerstrasse 8, 7270 Davos, Switzerland
    J Orthop Surg Res 5:32. 2010
  6. doi request reprint Chondrogenesis of human bone marrow-derived mesenchymal stem cells is modulated by complex mechanical stimulation and adenoviral-mediated overexpression of bone morphogenetic protein 2
    Alexander J Neumann
    Musculoskeletal Regeneration Program, AO Research Institute Davos, AO Foundation, Davos Platz, Switzerland
    Tissue Eng Part A 19:1285-94. 2013
  7. pmc Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway
    Zhen Li
    AO Research Institute, AO Foundation, Davos Platz, Switzerland
    J Cell Mol Med 14:1338-46. 2010
  8. doi request reprint Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin-polyurethane composites is modulated by frequency and amplitude of dynamic compression and shear stress
    Zhen Li
    AO Research Institute Davos, AO Foundation, Davos Platz, Switzerland
    Tissue Eng Part A 16:575-84. 2010
  9. doi request reprint The role of retinoic acid receptor inhibitor LE135 on the osteochondral differentiation of human bone marrow mesenchymal stem cells
    Zhen Li
    AO Research Institute, AO Foundation, Davos Platz 7270, Switzerland
    J Cell Biochem 112:963-70. 2011
  10. pmc Physical stimulation of chondrogenic cells in vitro: a review
    Sibylle Grad
    AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland
    Clin Orthop Relat Res 469:2764-72. 2011

Collaborators

Detail Information

Publications25

  1. doi request reprint WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
    Martin J Stoddart
    AO Research Institute Davos, Davos Platz, Switzerland
    Methods Mol Biol 740:21-5. 2011
    ..This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity...
  2. doi request reprint Cells and secretome--towards endogenous cell re-activation for cartilage repair
    Martin J Stoddart
    AO Research Institute Davos, 7270 Davos Platz, Switzerland Electronic address
    Adv Drug Deliv Rev 84:135-45. 2015
    ..Potential therapies and their effects on the repair of articular cartilage defects will be discussed, with a focus on the transition from classical cell therapy to the implantation of cell free matrices releasing specific cytokines. ..
  3. doi request reprint Cells and biomaterials in cartilage tissue engineering
    Martin J Stoddart
    Biomaterials and Tissue Engineering, AO Research Institute, Davos Platz, Switzerland
    Regen Med 4:81-98. 2009
    ..This review aims to provide an overview of potential cell sources and different scaffold materials, which are in development for cartilage tissue engineering...
  4. doi request reprint Cell viability assays: introduction
    Martin J Stoddart
    AO Research Institute Davos, Davos Platz, Switzerland
    Methods Mol Biol 740:1-6. 2011
    ..The cost, speed, and complexity of equipment required will all play a role in determining the assay used. This chapter aims to provide an overview of many of the assays available today...
  5. pmc A rapid method for the generation of uniform acellular bone explants: a technical note
    Katharina Jahn
    AO Research Institute, Clavadelerstrasse 8, 7270 Davos, Switzerland
    J Orthop Surg Res 5:32. 2010
    ..abstract:..
  6. doi request reprint Chondrogenesis of human bone marrow-derived mesenchymal stem cells is modulated by complex mechanical stimulation and adenoviral-mediated overexpression of bone morphogenetic protein 2
    Alexander J Neumann
    Musculoskeletal Regeneration Program, AO Research Institute Davos, AO Foundation, Davos Platz, Switzerland
    Tissue Eng Part A 19:1285-94. 2013
    ..However, BMP-2 overexpression consistently resulted in a trend toward decreased GAG/DNA ratios in both mechanical stimulated and unloaded groups...
  7. pmc Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway
    Zhen Li
    AO Research Institute, AO Foundation, Davos Platz, Switzerland
    J Cell Mol Med 14:1338-46. 2010
    ..Taken together, these findings suggest that mechanical load promotes chondrogenesis of hMSCs through TGF-beta pathway by up-regulating TGF-beta gene expression and protein synthesis...
  8. doi request reprint Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin-polyurethane composites is modulated by frequency and amplitude of dynamic compression and shear stress
    Zhen Li
    AO Research Institute Davos, AO Foundation, Davos Platz, Switzerland
    Tissue Eng Part A 16:575-84. 2010
    ..In conclusion, chondrogenesis of human MSCs induced by mechanical stimulation can be further enhanced by modifying frequency and compression...
  9. doi request reprint The role of retinoic acid receptor inhibitor LE135 on the osteochondral differentiation of human bone marrow mesenchymal stem cells
    Zhen Li
    AO Research Institute, AO Foundation, Davos Platz 7270, Switzerland
    J Cell Biochem 112:963-70. 2011
    ..Some cells were undergoing chondrogenesis and this was affected by LE135, while other cells underwent osteogenic differentiation and were not affected by LE135...
  10. pmc Physical stimulation of chondrogenic cells in vitro: a review
    Sibylle Grad
    AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland
    Clin Orthop Relat Res 469:2764-72. 2011
    ..As a result of the complexity of the load and motion patterns within an articulating joint, no bioreactor can completely recreate the in vivo situation...
  11. doi request reprint Improving chondrogenesis: potential and limitations of SOX9 gene transfer and mechanical stimulation for cartilage tissue engineering
    Laszlo Kupcsik
    AO Research Institute, Davos Platz, Switzerland
    Tissue Eng Part A 16:1845-55. 2010
    ..These findings provide insight into the mechanotransduction of mesenchymal stem cells and demonstrate the potential of a transcription factor in stem cell therapy...
  12. doi request reprint Homing of mesenchymal stem cells in induced degenerative intervertebral discs in a whole organ culture system
    Svenja Illien-Jünger
    AO Research Institute, Davos, Switzerland
    Spine (Phila Pa 1976) 37:1865-73. 2012
    ..Homing of human bone marrow-derived mesenchymal stem cells (BMSCs) was studied using ex vivo cultured bovine caudal intervertebral discs (IVDs)...
  13. ncbi request reprint Establishing a 3D ex vivo culture system for investigations of bone metabolism and biomaterial interactions
    R Geoff Richards
    AO Research Institute, Davos Platz, Switzerland
    ALTEX 24:56-9. 2007
    ..Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies...
  14. doi request reprint Enhanced adenovirus transduction of hMSCs using 3D hydrogel cell carriers
    Alexander J Neumann
    Musculoskeletal Regeneration Program, AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland
    Mol Biotechnol 53:207-16. 2013
    ..It therefore, might help increasing its administration in tissue engineering and clinical applications...
  15. doi request reprint TGFbeta3 and loading increases osteocyte survival in human cancellous bone cultured ex vivo
    Angharad E Simpson
    AO Research Institute, AO Foundation, Davos, Switzerland
    Cell Biochem Funct 27:23-9. 2009
    ..Further to this, the combination of daily loading and TGFbeta(3) administration produced superior osteocyte survival at the core centres when compared to loading or TGFbeta alone...
  16. doi request reprint Human Articular Cartilage Progenitor Cells Are Responsive to Mechanical Stimulation and Adenoviral-Mediated Overexpression of Bone-Morphogenetic Protein 2
    Alexander J Neumann
    AO Research Institute Davos, AO Foundation, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland Cardiff School of Biosciences, Cardiff University, CF10 3 AX, Cardiff, Wales, United Kingdom
    PLoS ONE 10:e0136229. 2015
    ..Over-expression of BMP-2, independent of load, led to an increase in markers associated with hypertropy. Taken together ACPCs represent a potential alterative cell source for cartilage tissue engineering applications. ..
  17. doi request reprint Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts
    Jan O Voss
    AO Research Institute Davos, 7270 Davos Platz, Switzerland Department of Oral and Maxillofacial Surgery Clinical Navigation, Charite Universitatsmedizin Berlin, Campus Virchow Clinic, 13353 Berlin, Germany
    Biomed Res Int 2015:714230. 2015
    ..In addition, undifferentiated hMSCs are able to regulate osteoblast differentiation; however, this regulatory function is lost upon osteogenic differentiation in vitro. This offers a novel approach for clinical cell therapy protocols. ..
  18. ncbi request reprint Tissue engineering for articular cartilage repair--the state of the art
    Brian Johnstone
    AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland
    Eur Cell Mater 25:248-67. 2013
    ..With this approach we are more likely to realise the clinical goal of treating both focal defects and even large-scale osteoarthritic degenerative changes in the joint...
  19. doi request reprint Epsilon-aminocaproic acid is a useful fibrin degradation inhibitor for cartilage tissue engineering
    Laszlo Kupcsik
    Biomaterials and Tissue Engineering Program, AO Research Institute, Davos Platz, Switzerland
    Tissue Eng Part A 15:2309-13. 2009
    ..Therefore, it is a useful plasmin inhibitor for cartilage tissue engineering with hMSCs...
  20. doi request reprint Mesenchymal Stem Cells Derived from Human Bone Marrow
    Oliver F W Gardner
    Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland
    Methods Mol Biol 1340:41-52. 2015
    ..This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the cells and characterisation of their multipotency...
  21. pmc In vitro osteogenic potential of human mesenchymal stem cells is predicted by Runx2/Sox9 ratio
    Claudia Loebel
    1 AO Research Institute Davos, Davos Platz, Switzerland
    Tissue Eng Part A 21:115-23. 2015
    ..During endochondral bone formation in vivo, Sox9 directly interacts with Runx2 and represses its activity; however, the role of Sox9 in direct osteogenesis in vitro has been largely overlooked...
  22. doi request reprint Three-dimensional culture and characterization of mononuclear cells from human bone marrow
    Jennifer J Bara
    AO Research Institute Davos, Davos Platz, Switzerland
    Cytotherapy 17:458-72. 2015
    ..The mononuclear cell fraction of bone marrow, inclusive of discrete stem cell populations, is not well characterized, and we currently lack suitable cell culture systems in which to culture and investigate the behavior of these cells...
  23. doi request reprint Concise review: Bone marrow-derived mesenchymal stem cells change phenotype following in vitro culture: implications for basic research and the clinic
    Jennifer J Bara
    AO Research Institute Davos, Davos Platz, 7270, Davos, Switzerland
    Stem Cells 32:1713-23. 2014
    ..Finally, we describe recent work attempting to model the MSC bone marrow niche, with focus on both basic research and clinical applications and consider the challenges associated with these new generation culture systems...
  24. doi request reprint The use of Reamer Irrigator Aspirator (RIA) autograft harvest in the treatment of critical-sized iliac wing defects in sheep: investigation of dexamethasone and beta-tricalcium phosphate augmentation
    Aswin Beck
    AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland
    Bone 53:554-65. 2013
    ..If avoidance of an iliac wing graft is desirable, or a reaming procedure is required, then a RIA graft or RIA graft plus β-TCP granules are as good as the current gold standard for this model...
  25. doi request reprint Role of HOXA9 and VEZF1 in endothelial biology
    Marco Bruderer
    Musculoskeletal Regeneration Program, AO Research Institute Davos, Davos, Switzerland
    J Vasc Res 50:265-78. 2013
    ..Due to their broad role in multiple aspects of endothelial biology, they might potentially become interesting targets for therapeutic manipulation of pathological blood vessel growth. ..