Affiliation: AO Research Institute
- Chondrocyte gene expression under applied surface motionSibylle Grad
Biomaterials and Tissue Engineering Program, AO Research Institute, Davos, Switzerland
Biorheology 43:259-69. 2006..These results demonstrate the beneficial effect of articular motion not only for stimulation of important lubricating molecules, but also for the preservation of the chondrocytic phenotype...
- Effects of simple and complex motion patterns on gene expression of chondrocytes seeded in 3D scaffoldsSibylle Grad
AO Research Institute, Davos Platz, Switzerland
Tissue Eng 12:3171-9. 2006..It is suggested that the oscillating ball drags the fluid into the joint space, thereby causing biophysical effects similar to those of fluid flow...
- Sliding motion modulates stiffness and friction coefficient at the surface of tissue engineered cartilageS Grad
AO Research Institute Davos, Switzerland
Osteoarthritis Cartilage 20:288-95. 2012..For better understanding of the molecule-function relationships, AFM was complemented with immunohistochemistry...
- [Chondrocytes - one cell type, different subpopulations : characteristics and behavior of different types of chondrocytes and implications for tissue engineering applications]S Grad
AO Forschungsinstitut Davos, Clavadelerstrasse 8, CH 7270, Davos, Schweiz
Orthopade 38:1038-44. 2009..In particular, an overview of recent results from comparative studies is presented, and possible consequences for the design of tissue engineering models are discussed...
- A combination of shear and dynamic compression leads to mechanically induced chondrogenesis of human mesenchymal stem cellsO Schätti
AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, Switzerland
Eur Cell Mater 22:214-25. 2011..Histological analysis detected sulphated glycosaminoglycan and collagen II only in the compression and shear group. The results obtained may provide insight into post-operative care after cell therapy involving mesenchymal stromal cells...
- Cell therapy for intervertebral disc repair: advancing cell therapy from bench to clinicsL M Benneker
AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos
Eur Cell Mater 27:5-11. 2014..Finally, appropriate large animal models and well-designed clinical studies will be required, particularly addressing safety aspects. ..
- Platelet-rich plasma induces annulus fibrosus cell proliferation and matrix productionT N Pirvu
AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland
Eur Spine J 23:745-53. 2014..Platelet-rich plasma (PRP) contains growth factors and creates a 3D structure upon clotting; PRP or platelet lysate (PL) might be considered for annulus fibrosus (AF) repair...
- Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cellsJ V Stoyanov
AO Research Institute Davos, Clavadelerstrasse, Switzerland
Eur Cell Mater 21:533-47. 2011..Trends in gene expression regulation were confirmed on the protein level using immunohistochemistry. We conclude that hypoxia and GDF5 may be suitable for directing MSCs towards the IVD-like phenotype...
- CCL5/RANTES is a key chemoattractant released by degenerative intervertebral discs in organ cultureG Pattappa
AO Research Institute Davos, Clavadelerstrasse 8, CH 7270 Davos
Eur Cell Mater 27:124-36; discussion 136. 2014..Therefore, these factors could be used to enhance stem/progenitor cell mobilisation in regenerative therapies for early stages of disc degeneration. ..
- Effect of reduced oxygen tension and long-term mechanical stimulation on chondrocyte-polymer constructsEllen Wernike
Biomaterials and Tissue Engineering Program, AO Research Institute, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland
Cell Tissue Res 331:473-83. 2008....
- A phenotypic comparison of intervertebral disc and articular cartilage cells in the ratCynthia R Lee
Biomaterials and Tissue Engineering Program, AO Research Institute, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland
Eur Spine J 16:2174-85. 2007..Future studies using different species are required to evaluate whether the expression of these molecules can be used to characterize NP cells and distinguish them from other chondrocyte-like cells...
- Chondrocytes seeded onto poly (L/DL-lactide) 80%/20% porous scaffolds: a biochemical evaluationSibylle Grad
Biochemistry and Cell Biology, AO Research Institute, 7270 Davos Platz, Switzerland
J Biomed Mater Res A 66:571-9. 2003..The limitations of the system are the diffusion of large amounts of matrix molecules into the culture medium and the dedifferentiation of the chondrocytes with prolonged time in culture...
- The use of biodegradable polyurethane scaffolds for cartilage tissue engineering: potential and limitationsSibylle Grad
Biochemistry and Cell Biology Unit, AO Research Institute, 7270 Davos Platz, Switzerland
Biomaterials 24:5163-71. 2003..However, due to the favourable mechanical properties of the polyurethane scaffold, stimulation of chondrocytes by mechanical loading can be considered in order to improve the formation of a functional cartilage-like extracellular matrix...
- Tribology approach to the engineering and study of articular cartilageMarkus A Wimmer
Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois 60612, USA
Tissue Eng 10:1436-45. 2004..The final design of the bioreactor will include four individual stations in line, which will facilitate the investigation of motion-initiated effects at the contacting surfaces in more detail...
- Surface motion upregulates superficial zone protein and hyaluronan production in chondrocyte-seeded three-dimensional scaffoldsSibylle Grad
Biochemistry and Cell Biology, AO Research Institute, Davos Platz, Switzerland
Tissue Eng 11:249-56. 2005..These results indicate that specific stimuli that mimic the kinematics of natural joints, such as articular motion, may promote the development of a functional articular surface-synovial interface...
- Effect of mechanical loading on mRNA levels of common endogenous controls in articular chondrocytes and intervertebral diskCynthia R Lee
Biochemistry and Cell Biology, AO Research Institute, Davos, Switzerland
Anal Biochem 341:372-5. 2005
- Fibrin-polyurethane composites for articular cartilage tissue engineering: a preliminary analysisCynthia R Lee
Biochemistry and Cell Biology, AO Research Institute, Davos, Switzerland
Tissue Eng 11:1562-73. 2005..Although further work is needed to optimize this system, the addition of fibrin hydrogel to encapsulate cells in the stiff, macroporous PU scaffold is a step forward in our approach to articular cartilage tissue engineering...
- Effects of immobilization and dynamic compression on intervertebral disc cell gene expression in vivoJeffery J Maclean
Department of Mechanical Engineering, University of Vermont, Burlington 05405 0156, USA
Spine (Phila Pa 1976) 28:973-81. 2003..An in vivo analysis of the intervertebral disc's cellular response to dynamic compression and immobilization was performed using a rat-tail model...
- Association of the asporin D14 allele with lumbar-disc degeneration in AsiansYou Qiang Song
Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China
Am J Hum Genet 82:744-7. 2008..70 (p = 0.000013). ASPN expression in vertebral discs increased with age and degeneration. Our results indicate ASPN is a LDD gene in Asians, and common risk factors may be considered for OA and LDD...
- An injectable cross-linked scaffold for nucleus pulposus regenerationDamien O Halloran
National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland
Biomaterials 29:438-47. 2008....