Research Topics
Species | S GradSummaryAffiliation: AO Research Institute Country: Switzerland Publications
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Detail Information
Publications
Effects of simple and complex motion patterns on gene expression of chondrocytes seeded in 3D scaffoldsSibylle Grad
AO Research Institute, Davos Platz, Switzerland
Tissue Eng 12:3171-9. 2006..It is suggested that the oscillating ball drags the fluid into the joint space, thereby causing biophysical effects similar to those of fluid flow...
Sliding motion modulates stiffness and friction coefficient at the surface of tissue engineered cartilageS Grad
AO Research Institute Davos, Switzerland
Osteoarthritis Cartilage 20:288-95. 2012..For better understanding of the molecule-function relationships, AFM was complemented with immunohistochemistry...- [Chondrocytes - one cell type, different subpopulations : characteristics and behavior of different types of chondrocytes and implications for tissue engineering applications]S Grad
AO Forschungsinstitut Davos, Clavadelerstrasse 8, CH 7270, Davos, Schweiz
Orthopade 38:1038-44. 2009..In particular, an overview of recent results from comparative studies is presented, and possible consequences for the design of tissue engineering models are discussed... - Chondrocyte gene expression under applied surface motionSibylle Grad
Biomaterials and Tissue Engineering Program, AO Research Institute, Davos, Switzerland
Biorheology 43:259-69. 2006..These results demonstrate the beneficial effect of articular motion not only for stimulation of important lubricating molecules, but also for the preservation of the chondrocytic phenotype... - A combination of shear and dynamic compression leads to mechanically induced chondrogenesis of human mesenchymal stem cellsO Schätti
AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, Switzerland
Eur Cell Mater 22:214-25. 2011..Histological analysis detected sulphated glycosaminoglycan and collagen II only in the compression and shear group. The results obtained may provide insight into post-operative care after cell therapy involving mesenchymal stromal cells... - Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cellsJ V Stoyanov
AO Research Institute Davos, Clavadelerstrasse, Switzerland
Eur Cell Mater 21:533-47. 2011..Trends in gene expression regulation were confirmed on the protein level using immunohistochemistry. We conclude that hypoxia and GDF5 may be suitable for directing MSCs towards the IVD-like phenotype... - Surface motion upregulates superficial zone protein and hyaluronan production in chondrocyte-seeded three-dimensional scaffoldsSibylle Grad
Biochemistry and Cell Biology, AO Research Institute, Davos Platz, Switzerland
Tissue Eng 11:249-56. 2005..These results indicate that specific stimuli that mimic the kinematics of natural joints, such as articular motion, may promote the development of a functional articular surface-synovial interface... - Chondrocytes seeded onto poly (L/DL-lactide) 80%/20% porous scaffolds: a biochemical evaluationSibylle Grad
Biochemistry and Cell Biology, AO Research Institute, 7270 Davos Platz, Switzerland
J Biomed Mater Res A 66:571-9. 2003..The limitations of the system are the diffusion of large amounts of matrix molecules into the culture medium and the dedifferentiation of the chondrocytes with prolonged time in culture... - Effect of reduced oxygen tension and long-term mechanical stimulation on chondrocyte-polymer constructsEllen Wernike
Biomaterials and Tissue Engineering Program, AO Research Institute, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland
Cell Tissue Res 331:473-83. 2008.... 
A phenotypic comparison of intervertebral disc and articular cartilage cells in the ratCynthia R Lee
Biomaterials and Tissue Engineering Program, AO Research Institute, Clavadelerstrasse 8, 7270, Davos Platz, Switzerland
Eur Spine J 16:2174-85. 2007..Future studies using different species are required to evaluate whether the expression of these molecules can be used to characterize NP cells and distinguish them from other chondrocyte-like cells...- The use of biodegradable polyurethane scaffolds for cartilage tissue engineering: potential and limitationsSibylle Grad
Biochemistry and Cell Biology Unit, AO Research Institute, 7270 Davos Platz, Switzerland
Biomaterials 24:5163-71. 2003..However, due to the favourable mechanical properties of the polyurethane scaffold, stimulation of chondrocytes by mechanical loading can be considered in order to improve the formation of a functional cartilage-like extracellular matrix... - Tribology approach to the engineering and study of articular cartilageMarkus A Wimmer
Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois 60612, USA
Tissue Eng 10:1436-45. 2004..The final design of the bioreactor will include four individual stations in line, which will facilitate the investigation of motion-initiated effects at the contacting surfaces in more detail... - Fibrin-polyurethane composites for articular cartilage tissue engineering: a preliminary analysisCynthia R Lee
Biochemistry and Cell Biology, AO Research Institute, Davos, Switzerland
Tissue Eng 11:1562-73. 2005..Although further work is needed to optimize this system, the addition of fibrin hydrogel to encapsulate cells in the stiff, macroporous PU scaffold is a step forward in our approach to articular cartilage tissue engineering... - Effect of mechanical loading on mRNA levels of common endogenous controls in articular chondrocytes and intervertebral diskCynthia R Lee
Biochemistry and Cell Biology, AO Research Institute, Davos, Switzerland
Anal Biochem 341:372-5. 2005 - Effects of immobilization and dynamic compression on intervertebral disc cell gene expression in vivoJeffery J Maclean
Department of Mechanical Engineering, University of Vermont, Burlington 05405-0156, USA
Spine 28:973-81. 2003..In studying the response of the disc to loading, it is necessary to look at both anabolic and catabolic pathways, and to consider strain history... - An injectable cross-linked scaffold for nucleus pulposus regenerationDamien O Halloran
National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland
Biomaterials 29:438-47. 2008.... - Association of the asporin D14 allele with lumbar-disc degeneration in AsiansYou Qiang Song
Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China
Am J Hum Genet 82:744-7. 2008..70 (p = 0.000013). ASPN expression in vertebral discs increased with age and degeneration. Our results indicate ASPN is a LDD gene in Asians, and common risk factors may be considered for OA and LDD...