R Ohlsson

Summary

Affiliation: Uppsala University
Country: Sweden

Publications

  1. ncbi request reprint Multiple cis elements within the Igf2/H19 insulator domain organize a distance-dependent silencer. A cautionary note
    Vasudeva Ginjala
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    J Biol Chem 277:5707-10. 2002
  2. ncbi request reprint CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease
    R Ohlsson
    Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Trends Genet 17:520-7. 2001
  3. ncbi request reprint The 4C technique: the 'Rosetta stone' for genome biology in 3D?
    Rolf Ohlsson
    Department of Development and Genetics, Norbyvagen 18A, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Curr Opin Cell Biol 19:321-5. 2007
  4. ncbi request reprint Genetics. Widespread monoallelic expression
    Rolf Ohlsson
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Science 318:1077-8. 2007
  5. ncbi request reprint [Epigenetics--cellular memories--affect disease development]
    Rolf Ohlsson
    Lakartidningen 103:919-25. 2006
  6. ncbi request reprint New twists on the epigenetics of CpG islands
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Genome Res 12:525-6. 2002
  7. ncbi request reprint Did genomic imprinting and X chromosome inactivation arise from stochastic expression?
    R Ohlsson
    Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Trends Genet 17:136-41. 2001
  8. ncbi request reprint A differentially methylated imprinting control region within the Kcnq1 locus harbors a methylation-sensitive chromatin insulator
    Chandrasekhar Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    J Biol Chem 277:18106-10. 2002
  9. ncbi request reprint Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions
    Zhihu Zhao
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, SE 752 36 Uppsala, Sweden
    Nat Genet 38:1341-7. 2006
  10. ncbi request reprint CpG methylation regulates the Igf2/H19 insulator
    C Holmgren
    Department of Development and Genetics, Uppsala University, , S-752 36, Uppsala, Sweden
    Curr Biol 11:1128-30. 2001

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Multiple cis elements within the Igf2/H19 insulator domain organize a distance-dependent silencer. A cautionary note
    Vasudeva Ginjala
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    J Biol Chem 277:5707-10. 2002
    ..We issue a cautionary note, therefore, that the interpretation of insulator/silencer data could be erroneous should the distance issue not be taken into consideration...
  2. ncbi request reprint CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease
    R Ohlsson
    Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Trends Genet 17:520-7. 2001
    ..CTCF emerges, therefore, as a central player in networks linking expression domains with epigenetics and cell growth regulation...
  3. ncbi request reprint The 4C technique: the 'Rosetta stone' for genome biology in 3D?
    Rolf Ohlsson
    Department of Development and Genetics, Norbyvagen 18A, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Curr Opin Cell Biol 19:321-5. 2007
    ..As this method is based on the unbiased identification of sequences interacting with specific baits, there are unique opportunities for unravelling the secrets of how the genome functions in 3D...
  4. ncbi request reprint Genetics. Widespread monoallelic expression
    Rolf Ohlsson
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Science 318:1077-8. 2007
  5. ncbi request reprint [Epigenetics--cellular memories--affect disease development]
    Rolf Ohlsson
    Lakartidningen 103:919-25. 2006
  6. ncbi request reprint New twists on the epigenetics of CpG islands
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Genome Res 12:525-6. 2002
  7. ncbi request reprint Did genomic imprinting and X chromosome inactivation arise from stochastic expression?
    R Ohlsson
    Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Trends Genet 17:136-41. 2001
    ..Two different mechanisms can accomplish regular monoallelic expression - genomic imprinting and gene counting...
  8. ncbi request reprint A differentially methylated imprinting control region within the Kcnq1 locus harbors a methylation-sensitive chromatin insulator
    Chandrasekhar Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    J Biol Chem 277:18106-10. 2002
    ..Moreover, we document that CpG methylation regulates this insulator function suggesting that a methylation-sensitive chromatin insulator is a common theme in the phenomenon of genomic imprinting...
  9. ncbi request reprint Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions
    Zhihu Zhao
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, SE 752 36 Uppsala, Sweden
    Nat Genet 38:1341-7. 2006
    ..These observations shed new light on development, cancer epigenetics and the evolution of imprinting...
  10. ncbi request reprint CpG methylation regulates the Igf2/H19 insulator
    C Holmgren
    Department of Development and Genetics, Uppsala University, , S-752 36, Uppsala, Sweden
    Curr Biol 11:1128-30. 2001
    ..Finally, the insulator function was strongly attenuated in stably maintained episomes. Collectively, these results provide the first experimental support that the H19 insulator function is regulated by CpG methylation...
  11. ncbi request reprint Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation
    Wenqiang Yu
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Nat Genet 36:1105-10. 2004
    ..We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions...
  12. pmc Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritance
    Vinod Pant
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Mol Cell Biol 24:3497-504. 2004
    ..Considering many other CTCF targets, we propose that site-specific interactions between various DNA-bound CTCF molecules may provide general focal points in the organization of looped chromatin domains involved in gene regulation...
  13. ncbi request reprint The paternal allele of the H19 gene is progressively silenced during early mouse development: the acetylation status of histones may be involved in the generation of variegated expression patterns
    K Svensson
    Department of Animal Development and Genetics, Uppsala University, Sweden
    Development 125:61-9. 1998
    ..We suggest that allele-specific silencing of H19 share some common features with chromatin-mediated silencing in position-effect variegation...
  14. pmc CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2
    Sreenivasulu Kurukuti
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Proc Natl Acad Sci U S A 103:10684-9. 2006
    ....
  15. pmc An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region
    Noopur Thakur
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Mol Cell Biol 24:7855-62. 2004
    ..Double-stranded RNA (dsRNA)-mediated RNA interference is unlikely to be involved, as the ICR is active irrespective of the simultaneous production of dsRNA from the genes it silences...
  16. doi request reprint High-resolution circular chromosome conformation capture assay
    Anita Göndör
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Nat Protoc 3:303-13. 2008
    ..Following optimization of enzyme digestions and amplification conditions, the protocol can be completed in 2-3 weeks...
  17. pmc The nucleotides responsible for the direct physical contact between the chromatin insulator protein CTCF and the H19 imprinting control region manifest parent of origin-specific long-distance insulation and methylation-free domains
    Vinod Pant
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Genes Dev 17:586-90. 2003
    ..Because CTCF interacts with other imprinting control regions, it emerges as a central factor responsible for interpreting and propagating gamete-derived epigenetic marks and for organizing epigenetically controlled expression domains...
  18. ncbi request reprint The kinetics of deregulation of expression by de novo methylation of the h19 imprinting control region in cancer cells
    Chandrasekhar Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Cancer Res 62:4545-8. 2002
    ..Our results document that a strong de novo methylation machinery leads to loss of methylation privilege states of H19 ICR to functionally manifest loss of insulator function in a matter of only a few days in human cancer cells...
  19. ncbi request reprint CTCF regulates asynchronous replication of the imprinted H19/Igf2 domain
    Rosita Bergstrom
    Department of Development and Genetics, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden
    Cell Cycle 6:450-4. 2007
    ..Here we show that maternal, but not paternal inheritance of a mutated H19 imprinting control region, lacking functional CTCF binding sites, underlies a late to early switch in replication timing of the maternal H19/Igf2 domain...
  20. pmc The binding sites for the chromatin insulator protein CTCF map to DNA methylation-free domains genome-wide
    Rituparna Mukhopadhyay
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Genome Res 14:1594-602. 2004
    ..As these targets are generally DNA methylation-free as determined by antibodies against 5-methylcytidine and a methyl-binding protein (MBD2), a CTCF-based network correlates with genome-wide epigenetic states...
  21. pmc Multiple nucleosome positioning sites regulate the CTCF-mediated insulator function of the H19 imprinting control region
    Meena Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Mol Cell Biol 22:3339-44. 2002
    ..We propose that the NPSs ensure the fidelity of the repressed status of the maternal Igf2 allele during development by constitutively maintaining availability of the CTCF target sites...
  22. pmc Transforming growth factor beta promotes complexes between Smad proteins and the CCCTC-binding factor on the H19 imprinting control region chromatin
    Rosita Bergstrom
    Ludwig Institute for Cancer Research, Uppsala University, SE 751 24 Uppsala, Sweden
    J Biol Chem 285:19727-37. 2010
    ..Because the CTCF-Smad complex is not essential for the chromatin insulator function of the H19 ICR, we propose that it can play a role in chromatin cross-talk organized by the H19 ICR...
  23. ncbi request reprint Mosaic allelic insulin-like growth factor 2 expression patterns reveal a link between Wilms' tumorigenesis and epigenetic heterogeneity
    R Ohlsson
    Department of Animal Development and Genetics, Uppsala University, Sweden
    Cancer Res 59:3889-92. 1999
    ..These data show that Wilms' tumorigenesis involves epigenetic heterogeneity as visualized by variable allelic IGF2 expression patterns...
  24. ncbi request reprint Loss of IGF2 imprinting: mechanisms and consequences
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Novartis Found Symp 262:108-21; discussion 121-4, 265-8. 2004
    ..We discuss here the possibility that pathological mechanisms leading to IGF2 LOI use different strategies with the neutralization of the H19 ICR insulator function as a common denominator...
  25. pmc Nonallelic transvection of multiple imprinted loci is organized by the H19 imprinting control region during germline development
    Kuljeet Singh Sandhu
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Genes Dev 23:2598-603. 2009
    ..We conclude that one locus can directly or indirectly pleiotropically influence epigenetic states of multiple regions on other chromosomes with which it interacts...
  26. ncbi request reprint Impressions of imprints
    R Ohlsson
    Department of Animal Development and Genetics, Uppsala University, Sweden
    Trends Genet 10:415-7. 1994
  27. ncbi request reprint Epigenetic variability and the evolution of human cancer
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Adv Cancer Res 88:145-68. 2003
    ....
  28. pmc Chromatin insulators and cohesins
    Anita Göndör
    Department of Development and Genetics, Uppsala University, Evolution Biology Centre, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    EMBO Rep 9:327-9. 2008
  29. pmc Enhanced sensitivity to IGF-II signaling links loss of imprinting of IGF2 to increased cell proliferation and tumor risk
    Atsushi Kaneda
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, 1064 Ross, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:20926-31. 2007
    ....
  30. ncbi request reprint DNA methylation and genomic imprinting: insights from cancer into epigenetic mechanisms
    Andrew P Feinberg
    Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 1064 Ross, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Semin Cancer Biol 12:389-98. 2002
    ..LOI may precede the development of cancer and may thus serve as a common marker for risk, but also as a model for understanding the developmental mechanism for normal imprinting...
  31. ncbi request reprint Poly(ADP-ribosyl)ation and epigenetics. Is CTCF PARt of the plot?
    Elena Klenova
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, UK
    Cell Cycle 4:96-101. 2005
    ....
  32. ncbi request reprint Loss of imprinting of Igf2 alters intestinal maturation and tumorigenesis in mice
    Takashi Sakatani
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 307:1976-8. 2005
    ..A similar shift in differentiation was seen in the normal colonic mucosa of humans with LOI. Thus, altered maturation of nonneoplastic tissue may be one mechanism by which epigenetic changes affect cancer risk...
  33. pmc Epigenetic regulation of TTF-I-mediated promoter-terminator interactions of rRNA genes
    Attila Nemeth
    Department of Biochemistry III, University of Regensburg, Regensburg, Germany
    EMBO J 27:1255-65. 2008
    ..Reporter assays show that TTF-I mediates this interaction, thereby linking topology and epigenetic regulation of the rRNA genes...
  34. ncbi request reprint CpG methylation of the IFNG gene as a mechanism to induce immunosuppression [correction of immunosupression] in tumor-infiltrating lymphocytes
    Peter C J Janson
    Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden
    J Immunol 181:2878-86. 2008
    ....
  35. pmc BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma
    Dmitri I Loukinov
    Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0760, USA
    Proc Natl Acad Sci U S A 99:6806-11. 2002
    ..Because BORIS bears the same DNA-binding domain that CTCF employs for recognition of methylation marks in soma, BORIS is a candidate protein for the elusive epigenetic reprogramming factor acting in the male germ line...
  36. ncbi request reprint The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer
    Elena M Klenova
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CQ4 3SQ, UK
    Semin Cancer Biol 12:399-414. 2002
    ..The sibling rivalry occasioned by aberrant expression of BORIS in cancer may interfere with normal functions of CTCF including growth suppression, and contribute to epigenetic dysregulation which is a common feature in human cancer...
  37. ncbi request reprint Targeted regulation of imprinted genes by synthetic zinc-finger transcription factors
    Y Jouvenot
    Sangamo BioSciences, Inc, Point Richmond Tech Center, Richmond, CA 94804, USA
    Gene Ther 10:513-22. 2003
    ....
  38. ncbi request reprint A CTCF-binding silencer regulates the imprinted genes AWT1 and WT1-AS and exhibits sequential epigenetic defects during Wilms' tumourigenesis
    Anne L Hancock
    Cancer and Leukaemia in Childhood, Sargent Research Unit, Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, University Walk, Bristol, UK
    Hum Mol Genet 16:343-54. 2007
    ..We propose a model wherein imprinting defects at chromosome 11p13 may contribute to Wilms' tumourigenesis...
  39. pmc CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wide
    Igor Chernukhin
    Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Mol Cell Biol 27:1631-48. 2007
    ..We discuss the possible implications of our observations with respect to plausible mechanisms of transcriptional regulation via a CTCF-mediated direct link of LS Pol II to the DNA...
  40. ncbi request reprint Transcription in the loop
    Anita Göndör
    Nat Genet 38:1229-30. 2006
  41. pmc CTCF is conserved from Drosophila to humans and confers enhancer blocking of the Fab-8 insulator
    Hanlim Moon
    Section of Molecular Pathology, Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    EMBO Rep 6:165-70. 2005
    ..Therefore, the enhancer-blocking protein CTCF and, most probably, the mechanism of enhancer blocking mediated by this remarkably versatile factor are conserved from Drosophila to humans...
  42. ncbi request reprint Familial cases of point mutations in the XIST promoter reveal a correlation between CTCF binding and pre-emptive choices of X chromosome inactivation
    Elena M Pugacheva
    Molecular Pathology Section, Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Twinbrook I Rm 1417, Rockville, MD 20852, USA
    Hum Mol Genet 14:953-65. 2005
    ..These observations are in keeping with the possibility that the choice of X chromosome inactivation reflects stabilization of a higher order chromatin conformation impinging on the CTCF-XIST promoter complex...
  43. ncbi request reprint The epigenetic progenitor origin of human cancer
    Andrew P Feinberg
    Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
    Nat Rev Genet 7:21-33. 2006
    ..Therefore, non-neoplastic but epigenetically disrupted stem/progenitor cells might be a crucial target for cancer risk assessment and chemoprevention...
  44. pmc CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin
    Les J Burke
    Institute for Genetics, Justus Liebig Universitaet Giessen, Giessen, Germany
    EMBO J 24:3291-300. 2005
    ..In contrast, the enhancer/promoter loop of the same locus is lost in mitosis. This may provide a novel form of epigenetic memory during cell division...
  45. pmc FOXP3 promoter demethylation reveals the committed Treg population in humans
    Peter C J Janson
    Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 3:e1612. 2008
    ....