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Genomes and Genes | R OhlssonSummaryAffiliation: Uppsala University Country: Sweden Publications
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Publications
Multiple cis elements within the Igf2/H19 insulator domain organize a distance-dependent silencer. A cautionary noteVasudeva Ginjala
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, , S-752 36 Uppsala, Sweden
J Biol Chem 277:5707-10. 2002..We issue a cautionary note, therefore, that the interpretation of insulator/silencer data could be erroneous should the distance issue not be taken into consideration...- CTCF is a uniquely versatile transcription regulator linked to epigenetics and diseaseR Ohlsson
Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Trends Genet 17:520-7. 2001..CTCF emerges, therefore, as a central player in networks linking expression domains with epigenetics and cell growth regulation... - The 4C technique: the 'Rosetta stone' for genome biology in 3D?Rolf Ohlsson
Department of Development and Genetics, Norbyvagen 18A, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Curr Opin Cell Biol 19:321-5. 2007..As this method is based on the unbiased identification of sequences interacting with specific baits, there are unique opportunities for unravelling the secrets of how the genome functions in 3D... - Genetics. Widespread monoallelic expressionRolf Ohlsson
Department of Development and Genetics, Uppsala University, , S-752 36 Uppsala, Sweden
Science 318:1077-8. 2007 - [Epigenetics--cellular memories--affect disease development]Rolf Ohlsson
Lakartidningen 103:919-25. 2006 - New twists on the epigenetics of CpG islandsRolf Ohlsson
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, , S-752 36 Uppsala, Sweden
Genome Res 12:525-6. 2002 - Did genomic imprinting and X chromosome inactivation arise from stochastic expression?R Ohlsson
Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Trends Genet 17:136-41. 2001..Two different mechanisms can accomplish regular monoallelic expression - genomic imprinting and gene counting... - A differentially methylated imprinting control region within the Kcnq1 locus harbors a methylation-sensitive chromatin insulatorChandrasekhar Kanduri
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
J Biol Chem 277:18106-10. 2002..Moreover, we document that CpG methylation regulates this insulator function suggesting that a methylation-sensitive chromatin insulator is a common theme in the phenomenon of genomic imprinting... - Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactionsZhihu Zhao
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, , SE-752 36 Uppsala, Sweden
Nat Genet 38:1341-7. 2006..These observations shed new light on development, cancer epigenetics and the evolution of imprinting... - CpG methylation regulates the Igf2/H19 insulatorC Holmgren
Department of Development and Genetics, Uppsala University, , S-752 36, Uppsala, Sweden
Curr Biol 11:1128-30. 2001..Finally, the insulator function was strongly attenuated in stably maintained episomes. Collectively, these results provide the first experimental support that the H19 insulator function is regulated by CpG methylation... - Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulationWenqiang Yu
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Nat Genet 36:1105-10. 2004..We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions... 
Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritanceVinod Pant
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Mol Cell Biol 24:3497-504. 2004..Considering many other CTCF targets, we propose that site-specific interactions between various DNA-bound CTCF molecules may provide general focal points in the organization of looped chromatin domains involved in gene regulation...- The paternal allele of the H19 gene is progressively silenced during early mouse development: the acetylation status of histones may be involved in the generation of variegated expression patternsK Svensson
Department of Animal Development and Genetics, Uppsala University, Sweden
Development 125:61-9. 1998..We suggest that allele-specific silencing of H19 share some common features with chromatin-mediated silencing in position-effect variegation... - CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2Sreenivasulu Kurukuti
Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Proc Natl Acad Sci U S A 103:10684-9. 2006.... - An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control regionNoopur Thakur
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Mol Cell Biol 24:7855-62. 2004..Double-stranded RNA (dsRNA)-mediated RNA interference is unlikely to be involved, as the ICR is active irrespective of the simultaneous production of dsRNA from the genes it silences... 
High-resolution circular chromosome conformation capture assayAnita Göndör
Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Nat Protoc 3:303-13. 2008..Following optimization of enzyme digestions and amplification conditions, the protocol can be completed in 2-3 weeks...- The nucleotides responsible for the direct physical contact between the chromatin insulator protein CTCF and the H19 imprinting control region manifest parent of origin-specific long-distance insulation and methylation-free domainsVinod Pant
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Genes Dev 17:586-90. 2003..Because CTCF interacts with other imprinting control regions, it emerges as a central factor responsible for interpreting and propagating gamete-derived epigenetic marks and for organizing epigenetically controlled expression domains... - The kinetics of deregulation of expression by de novo methylation of the h19 imprinting control region in cancer cellsChandrasekhar Kanduri
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Cancer Res 62:4545-8. 2002..Our results document that a strong de novo methylation machinery leads to loss of methylation privilege states of H19 ICR to functionally manifest loss of insulator function in a matter of only a few days in human cancer cells... - CTCF regulates asynchronous replication of the imprinted H19/Igf2 domainRosita Bergstrom
Department of Development and Genetics, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden
Cell Cycle 6:450-4. 2007..Here we show that maternal, but not paternal inheritance of a mutated H19 imprinting control region, lacking functional CTCF binding sites, underlies a late to early switch in replication timing of the maternal H19/Igf2 domain... - The binding sites for the chromatin insulator protein CTCF map to DNA methylation-free domains genome-wideRituparna Mukhopadhyay
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, , S-752 36 Uppsala, Sweden
Genome Res 14:1594-602. 2004..As these targets are generally DNA methylation-free as determined by antibodies against 5-methylcytidine and a methyl-binding protein (MBD2), a CTCF-based network correlates with genome-wide epigenetic states... - Multiple nucleosome positioning sites regulate the CTCF-mediated insulator function of the H19 imprinting control regionMeena Kanduri
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Mol Cell Biol 22:3339-44. 2002..We propose that the NPSs ensure the fidelity of the repressed status of the maternal Igf2 allele during development by constitutively maintaining availability of the CTCF target sites... - Transforming growth factor beta promotes complexes between Smad proteins and the CCCTC-binding factor on the H19 imprinting control region chromatinRosita Bergstrom
Ludwig Institute for Cancer Research, Uppsala University, SE 751 24 Uppsala, Sweden
J Biol Chem 285:19727-37. 2010..Because the CTCF-Smad complex is not essential for the chromatin insulator function of the H19 ICR, we propose that it can play a role in chromatin cross-talk organized by the H19 ICR... - Mosaic allelic insulin-like growth factor 2 expression patterns reveal a link between Wilms' tumorigenesis and epigenetic heterogeneityR Ohlsson
Department of Animal Development and Genetics, Uppsala University, Sweden
Cancer Res 59:3889-92. 1999..These data show that Wilms' tumorigenesis involves epigenetic heterogeneity as visualized by variable allelic IGF2 expression patterns... - Loss of IGF2 imprinting: mechanisms and consequencesRolf Ohlsson
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, , S-752 36 Uppsala, Sweden
Novartis Found Symp 262:108-21; discussion 121-4, 265-8. 2004..We discuss here the possibility that pathological mechanisms leading to IGF2 LOI use different strategies with the neutralization of the H19 ICR insulator function as a common denominator... - Nonallelic transvection of multiple imprinted loci is organized by the H19 imprinting control region during germline developmentKuljeet Singh Sandhu
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Genes Dev 23:2598-603. 2009..We conclude that one locus can directly or indirectly pleiotropically influence epigenetic states of multiple regions on other chromosomes with which it interacts... - Impressions of imprintsR Ohlsson
Department of Animal Development and Genetics, Uppsala University, Sweden
Trends Genet 10:415-7. 1994 - Epigenetic variability and the evolution of human cancerRolf Ohlsson
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S-752 36 Uppsala, Sweden
Adv Cancer Res 88:145-68. 2003.... - Chromatin insulators and cohesinsAnita Göndör
Department of Development and Genetics, Uppsala University, Evolution Biology Centre, Norbyvagen 18A, S 752 36 Uppsala, Sweden
EMBO Rep 9:327-9. 2008 - Enhanced sensitivity to IGF-II signaling links loss of imprinting of IGF2 to increased cell proliferation and tumor riskAtsushi Kaneda
Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, 1064 Ross, 720 Rutland Avenue, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 104:20926-31. 2007.... - DNA methylation and genomic imprinting: insights from cancer into epigenetic mechanismsAndrew P Feinberg
Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 1064 Ross, 720 Rutland Avenue, Baltimore, MD 21205, USA
Semin Cancer Biol 12:389-98. 2002..LOI may precede the development of cancer and may thus serve as a common marker for risk, but also as a model for understanding the developmental mechanism for normal imprinting... - Poly(ADP-ribosyl)ation and epigenetics. Is CTCF PARt of the plot?Elena Klenova
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, UK
Cell Cycle 4:96-101. 2005.... - Loss of imprinting of Igf2 alters intestinal maturation and tumorigenesis in miceTakashi Sakatani
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Science 307:1976-8. 2005..A similar shift in differentiation was seen in the normal colonic mucosa of humans with LOI. Thus, altered maturation of nonneoplastic tissue may be one mechanism by which epigenetic changes affect cancer risk... - Epigenetic regulation of TTF-I-mediated promoter-terminator interactions of rRNA genesAttila Nemeth
Department of Biochemistry III, University of Regensburg, Regensburg, Germany
EMBO J 27:1255-65. 2008..Reporter assays show that TTF-I mediates this interaction, thereby linking topology and epigenetic regulation of the rRNA genes... - CpG methylation of the IFNG gene as a mechanism to induce immunosuppression [correction of immunosupression] in tumor-infiltrating lymphocytesPeter C J Janson
Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden
J Immunol 181:2878-86. 2008.... - BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the somaDmitri I Loukinov
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0760, USA
Proc Natl Acad Sci U S A 99:6806-11. 2002..Because BORIS bears the same DNA-binding domain that CTCF employs for recognition of methylation marks in soma, BORIS is a candidate protein for the elusive epigenetic reprogramming factor acting in the male germ line... - The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancerElena M Klenova
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CQ4 3SQ, UK
Semin Cancer Biol 12:399-414. 2002..The sibling rivalry occasioned by aberrant expression of BORIS in cancer may interfere with normal functions of CTCF including growth suppression, and contribute to epigenetic dysregulation which is a common feature in human cancer... - Targeted regulation of imprinted genes by synthetic zinc-finger transcription factorsY Jouvenot
Sangamo BioSciences, Inc, Point Richmond Tech Center, Richmond, CA 94804, USA
Gene Ther 10:513-22. 2003.... - A CTCF-binding silencer regulates the imprinted genes AWT1 and WT1-AS and exhibits sequential epigenetic defects during Wilms' tumourigenesisAnne L Hancock
Cancer and Leukaemia in Childhood, Sargent Research Unit, Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, University Walk, Bristol, UK
Hum Mol Genet 16:343-54. 2007..We propose a model wherein imprinting defects at chromosome 11p13 may contribute to Wilms' tumourigenesis... - CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wideIgor Chernukhin
Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
Mol Cell Biol 27:1631-48. 2007..We discuss the possible implications of our observations with respect to plausible mechanisms of transcriptional regulation via a CTCF-mediated direct link of LS Pol II to the DNA... - Transcription in the loopAnita Göndör
Nat Genet 38:1229-30. 2006 - CTCF is conserved from Drosophila to humans and confers enhancer blocking of the Fab-8 insulatorHanlim Moon
Section of Molecular Pathology, Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
EMBO Rep 6:165-70. 2005..Therefore, the enhancer-blocking protein CTCF and, most probably, the mechanism of enhancer blocking mediated by this remarkably versatile factor are conserved from Drosophila to humans... - Familial cases of point mutations in the XIST promoter reveal a correlation between CTCF binding and pre-emptive choices of X chromosome inactivationElena M Pugacheva
Molecular Pathology Section, Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Twinbrook I Rm 1417, Rockville, MD 20852, USA
Hum Mol Genet 14:953-65. 2005..These observations are in keeping with the possibility that the choice of X chromosome inactivation reflects stabilization of a higher order chromatin conformation impinging on the CTCF-XIST promoter complex... - The epigenetic progenitor origin of human cancerAndrew P Feinberg
Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
Nat Rev Genet 7:21-33. 2006..Therefore, non-neoplastic but epigenetically disrupted stem/progenitor cells might be a crucial target for cancer risk assessment and chemoprevention... - CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatinLes J Burke
Institute for Genetics, Justus Liebig Universitaet Giessen, Giessen, Germany
EMBO J 24:3291-300. 2005..In contrast, the enhancer/promoter loop of the same locus is lost in mitosis. This may provide a novel form of epigenetic memory during cell division... - FOXP3 promoter demethylation reveals the committed Treg population in humansPeter C J Janson
Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden
PLoS ONE 3:e1612. 2008....