Patrick Micke

Summary

Affiliation: Uppsala University
Country: Sweden

Publications

  1. ncbi A fluid cover medium provides superior morphology and preserves RNA integrity in tissue sections for laser microdissection and pressure catapulting
    Patrick Micke
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    J Pathol 202:130-8. 2004
  2. ncbi Exploring the tumour environment: cancer-associated fibroblasts as targets in cancer therapy
    Patrick Micke
    Department of Genetics and Pathology, Uppsala University, S 751 85, Uppsala, Sweden
    Expert Opin Ther Targets 9:1217-33. 2005
  3. ncbi Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues
    Kenneth Wester
    Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Lab Invest 83:889-99. 2003
  4. doi Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis, and tissue microarray validation
    Johan Botling
    Departments of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Clin Cancer Res 19:194-204. 2013
  5. pmc Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity
    Markus Rasmussen
    Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Akademiska sjukhuset, SE 751 85 Uppsala, Sweden
    Genome Biol 12:R108. 2011
  6. doi Gene copy number aberrations are associated with survival in histologic subgroups of non-small cell lung cancer
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    J Thorac Oncol 6:1833-40. 2011
  7. pmc Data-driven unbiased curation of the TP53 tumor suppressor gene mutation database and validation by ultradeep sequencing of human tumors
    Karolina Edlund
    Department of Immunology, and SciLifeLab Uppsala, Uppsala University, SE 751 85 Uppsala, Sweden
    Proc Natl Acad Sci U S A 109:9551-6. 2012
  8. doi CD99 is a novel prognostic stromal marker in non-small cell lung cancer
    Karolina Edlund
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Int J Cancer 131:2264-73. 2012
  9. pmc KRAS analysis in colorectal carcinoma: analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice
    Magnus Sundstrom
    Molecular Pathology Unit, Department of Genetics and Pathology, Uppsala University, SE 751 85 Uppsala, Sweden
    BMC Cancer 10:660. 2010
  10. doi Fresh frozen tissue: RNA extraction and quality control
    Johan Botling
    Rudbeck Laboratory, Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Methods Mol Biol 675:405-13. 2011

Collaborators

Detail Information

Publications20

  1. ncbi A fluid cover medium provides superior morphology and preserves RNA integrity in tissue sections for laser microdissection and pressure catapulting
    Patrick Micke
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    J Pathol 202:130-8. 2004
    ..This not only facilitates established procedures, but will also extend the application to tissues that require superior morphological resolution...
  2. ncbi Exploring the tumour environment: cancer-associated fibroblasts as targets in cancer therapy
    Patrick Micke
    Department of Genetics and Pathology, Uppsala University, S 751 85, Uppsala, Sweden
    Expert Opin Ther Targets 9:1217-33. 2005
    ..It is likely that the continued characterisation of this interaction, and the molecular identification of key mediators, will provide insights into tumour biology and suggest novel therapeutic options...
  3. ncbi Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues
    Kenneth Wester
    Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Lab Invest 83:889-99. 2003
    ..We conclude that ZBF is superior to NBF for analysis of DNA and protein expression. Fixation of tissues in ZBF may also be an alternative strategy to freeze storage of tissue specimens, eg, in future bio-banks...
  4. doi Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis, and tissue microarray validation
    Johan Botling
    Departments of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Clin Cancer Res 19:194-204. 2013
    ..So far, the value of these multigene signatures in clinical practice is unclear, and the biologic importance of individual genes is difficult to assess, as the published signatures virtually do not overlap...
  5. pmc Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity
    Markus Rasmussen
    Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Akademiska sjukhuset, SE 751 85 Uppsala, Sweden
    Genome Biol 12:R108. 2011
    ..Analysis of cancer samples indicates that TAPS is well suited to investigate samples with aneuploidy and tumor heterogeneity, which is commonly found in many types of solid tumors...
  6. doi Gene copy number aberrations are associated with survival in histologic subgroups of non-small cell lung cancer
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    J Thorac Oncol 6:1833-40. 2011
    ..To maximize the chance to identify clinically relevant events, we applied a strategy involving two prognostically extreme patient groups...
  7. pmc Data-driven unbiased curation of the TP53 tumor suppressor gene mutation database and validation by ultradeep sequencing of human tumors
    Karolina Edlund
    Department of Immunology, and SciLifeLab Uppsala, Uppsala University, SE 751 85 Uppsala, Sweden
    Proc Natl Acad Sci U S A 109:9551-6. 2012
    ..Thus, by combining statistical and experimental analyses, we provide a curated mutation database for TP53 mutations and a framework for mutation database analysis...
  8. doi CD99 is a novel prognostic stromal marker in non-small cell lung cancer
    Karolina Edlund
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Int J Cancer 131:2264-73. 2012
    ..The results support the concept that stromal properties have an important impact on tumour progression...
  9. pmc KRAS analysis in colorectal carcinoma: analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice
    Magnus Sundstrom
    Molecular Pathology Unit, Department of Genetics and Pathology, Uppsala University, SE 751 85 Uppsala, Sweden
    BMC Cancer 10:660. 2010
    ..Several procedures to detect KRAS mutations have been developed. However, the analytical sensitivity and specificity of these assays on routine clinical samples are not yet fully characterised...
  10. doi Fresh frozen tissue: RNA extraction and quality control
    Johan Botling
    Rudbeck Laboratory, Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Methods Mol Biol 675:405-13. 2011
    ..To this end, we here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis...
  11. doi Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue
    Johan Botling
    Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Diagn Mol Pathol 18:44-52. 2009
    ..In conclusion, degradation of RNA in frozen tissue occurs first after several minutes of thawing. Already minimal decrease in RNA quality may result in significant changes in gene expression patterns in clinical tissue samples...
  12. doi Biobanking of fresh frozen tissue from clinical surgical specimens: transport logistics, sample selection, and histologic characterization
    Johan Botling
    Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Methods Mol Biol 675:299-306. 2011
    ..Also, knowledge of the histomorphology of the frozen tissue sample - tumor cell content, stromal components, and presence of necrosis - is pivotal before entering a biobank case into costly molecular profiling studies...
  13. pmc Quantification of normal cell fraction and copy number neutral LOH in clinical lung cancer samples using SNP array data
    Hanna Göransson
    Department of Medical Sciences, Uppsala University, Uppsala, Sweden
    PLoS ONE 4:e6057. 2009
    ..Since tumor tissue invariably contains genetically normal stromal cells, this may lead to a failure to detect aberrations in the tumor cells...
  14. doi A novel strategy based on histological protein profiling in-silico for identifying potential biomarkers in urinary bladder cancer
    M Ulrika Segersten
    Department of Surgical Sciences Urology, Uppsala University Hospital, Uppsala, Sweden
    BJU Int 104:1780-5. 2009
    ..To screen a publicly available immunohistochemistry (IHC) based web-atlas, to identify key proteins in bladder cancer that might serve as potential biomarkers...
  15. ncbi Tumour-stroma interaction: cancer-associated fibroblasts as novel targets in anti-cancer therapy?
    Patrick Micke
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    Lung Cancer 45:S163-75. 2004
    ..Finally, a laser-capture- and microarray-based approach is presented, which comprehensively characterises carcinoma-associated fibroblasts in their in vivo environment for the identification of potential targets for anti-cancer therapy...
  16. ncbi In vitro characterization of the cytokine profile of the epithelial cell rests of Malassez
    Mitsuhiro Ohshima
    Department of Biochemistry, Nihon University School of Dentistry, 1 8 13 Kanda Surugadai, Chiyoda ku, Tokyo, Japan
    J Periodontol 79:912-9. 2008
    ..The aim of this study was to establish primary cell cultures of human ERM, characterize the cytokine profile, and compare it to other periodontal cell entities...
  17. ncbi In situ identification of genes regulated specifically in fibroblasts of human basal cell carcinoma
    Patrick Micke
    Department of Oncology Pathology, Cancer Centrum Karolinska, Karolinska Institutet, Stockholm, Sweden
    J Invest Dermatol 127:1516-23. 2007
    ..The results suggest a specific expression profile of CAFs in BCC possibly accounting for disease-specific pathological roles...
  18. ncbi Dynamic changes in the expression of DEP-1 and other PDGF receptor-antagonizing PTPs during onset and termination of neointima formation
    Kai Kappert
    Cancer Centrum Karolinska, Department of Oncology Pathology, Karolinska Institutet, 17176 Stockholm, Sweden
    FASEB J 21:523-34. 2007
    ..In more general terms, the observations indicate transcriptional regulation of PTPs as an important mechanism for controlling onset and termination of RTK-dependent tissue remodeling...
  19. ncbi Laser-assisted cell microdissection using the PALM system
    Patrick Micke
    Cancer Center Karolinska, Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden
    Methods Mol Biol 293:151-66. 2005
    ..The described procedures emphasize preparation of tissue, laser microdissection including catapulting of cells, and extraction of RNA and DNA. Downstream experiments for validation are also shown...
  20. ncbi Downregulation of bradykinin B2 receptor in human fibroblasts during prolonged agonist exposure
    Andree Blaukat
    Institute of Pharmacology, University of Heidelberg, Germany
    Am J Physiol Heart Circ Physiol 284:H1909-16. 2003
    ..e., by attenuation of de novo synthesis and by reduction of receptor stability...