O Korsgren

Summary

Affiliation: Uppsala University
Country: Sweden

Publications

  1. ncbi Toward clinical trials of islet xenotransplantation
    Olle Korsgren
    Department of Clinical Immunology and Transfusion Medicine, Uppsala University Hospital, Uppsala, Sweden
    Xenotransplantation 10:289-92. 2003
  2. pmc Decreased defluorination using the novel beta-cell imaging agent [18F]FE-DTBZ-d4 in pigs examined by PET
    Mahabuba Jahan
    Karolinska Institutet, Department of Clinical Neuroscience, Centre for Psychiatry Research, Building R5 U1, Karolinska University Hospital, SE 171 76, Stockholm, Sweden
    EJNMMI Res 1:33. 2011
  3. doi Improving islet transplantation: a road map for a widespread application for the cure of persons with type I diabetes
    Olle Korsgren
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Sweden
    Curr Opin Organ Transplant 14:683-7. 2009
  4. ncbi Current status of clinical islet transplantation
    Olle Korsgren
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplantation 79:1289-93. 2005
  5. ncbi Optimising islet engraftment is critical for successful clinical islet transplantation
    O Korsgren
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, Rudbeck Laboratory, C11, University Hospital, SE 751 85, Uppsala, Sweden
    Diabetologia 51:227-32. 2008
  6. doi Co-transplantation of human and pig islets
    Olle Korsgren
    Department of Clinical Immunology, University of Uppsala, Sweden
    Xenotransplantation 15:112. 2008
  7. doi Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation
    H Brandhorst
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Uppsala, Sweden
    Cell Transplant 19:3-8. 2010
  8. ncbi Key factors for human islet isolation and clinical transplantation
    M Goto
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplant Proc 37:1315-6. 2005
  9. ncbi A distinct Th1 immune response precedes the described Th2 response in islet xenograft rejection
    Henrik Krook
    Division of Clinical Immunology, Uppsala University, Uppsala, Sweden
    Diabetes 51:79-86. 2002
  10. ncbi Delayed type hypersensitivity-associated cytokines in islet xenotransplantation: limited efficacy of interleukin-2- and tumor necrosis factor-alpha-blockade in interferon-gamma receptor-deficient mice
    B Benda
    Department of Oncology, Radiology, and Clinical Immunology, Uppsala University, Sweden
    Xenotransplantation 7:206-13. 2000

Research Grants

Detail Information

Publications90

  1. ncbi Toward clinical trials of islet xenotransplantation
    Olle Korsgren
    Department of Clinical Immunology and Transfusion Medicine, Uppsala University Hospital, Uppsala, Sweden
    Xenotransplantation 10:289-92. 2003
  2. pmc Decreased defluorination using the novel beta-cell imaging agent [18F]FE-DTBZ-d4 in pigs examined by PET
    Mahabuba Jahan
    Karolinska Institutet, Department of Clinical Neuroscience, Centre for Psychiatry Research, Building R5 U1, Karolinska University Hospital, SE 171 76, Stockholm, Sweden
    EJNMMI Res 1:33. 2011
    ..abstract:..
  3. doi Improving islet transplantation: a road map for a widespread application for the cure of persons with type I diabetes
    Olle Korsgren
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Sweden
    Curr Opin Organ Transplant 14:683-7. 2009
    ..However, results have, in general, been disappointing when transferred to large animal models or to humans...
  4. ncbi Current status of clinical islet transplantation
    Olle Korsgren
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplantation 79:1289-93. 2005
    ..A conceivable strategy to achieve this goal in clinical islet transplantation is discussed...
  5. ncbi Optimising islet engraftment is critical for successful clinical islet transplantation
    O Korsgren
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, Rudbeck Laboratory, C11, University Hospital, SE 751 85, Uppsala, Sweden
    Diabetologia 51:227-32. 2008
    ..A novel view on the engraftment of intraportally transplanted islets is presented that could explain the low efficacy of the procedure...
  6. doi Co-transplantation of human and pig islets
    Olle Korsgren
    Department of Clinical Immunology, University of Uppsala, Sweden
    Xenotransplantation 15:112. 2008
  7. doi Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation
    H Brandhorst
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Uppsala, Sweden
    Cell Transplant 19:3-8. 2010
    ..The results suggest that Liberase is more efficient for pancreas dissociation than collagenase NB1 but seems to be more harmful to exocrine cells and islet tissue...
  8. ncbi Key factors for human islet isolation and clinical transplantation
    M Goto
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplant Proc 37:1315-6. 2005
    ..To further improve the outcome of clinical islet transplantation analysis of the impact of donor- and process-related factors could be of great importance...
  9. ncbi A distinct Th1 immune response precedes the described Th2 response in islet xenograft rejection
    Henrik Krook
    Division of Clinical Immunology, Uppsala University, Uppsala, Sweden
    Diabetes 51:79-86. 2002
    ....
  10. ncbi Delayed type hypersensitivity-associated cytokines in islet xenotransplantation: limited efficacy of interleukin-2- and tumor necrosis factor-alpha-blockade in interferon-gamma receptor-deficient mice
    B Benda
    Department of Oncology, Radiology, and Clinical Immunology, Uppsala University, Sweden
    Xenotransplantation 7:206-13. 2000
    ..Nevertheless, in a majority of animals, other cytokines eventually substitute for the lack of IFN-gamma, TNF-alpha and interleukin-2...
  11. ncbi Expression of islet amyloid polypeptide in fetal and adult porcine and human pancreatic islet cells
    A Lukinius
    Department of Pathology, University of Uppsala, Sweden
    Endocrinology 137:5319-25. 1996
    ..The substance is more widely distributed within the pancreatic islet cell types than are any of the other islet hormones, which presumably has functional implications...
  12. ncbi Low molecular weight dextran sulfate: a strong candidate drug to block IBMIR in clinical islet transplantation
    H Johansson
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Am J Transplant 6:305-12. 2006
    ..The toxicity was assessed using a glucose challenge test and the pharmacokinetics was tested in the nonhuman primate model. Here, we present a tentative protocol for using LMW-DS in clinical islet transplantation...
  13. ncbi Production of tissue factor by pancreatic islet cells as a trigger of detrimental thrombotic reactions in clinical islet transplantation
    L Moberg
    Department of Radiology, Oncology, and Clinical Immunology, Division of Clinical Immunology, Rudbeck Laboratory, Uppsala, Sweden
    Lancet 360:2039-45. 2002
    ..We investigated the possible mechanism and treatment of such thrombotic reactions...
  14. ncbi Human islet separation utilizing a closed automated purification system
    A S Friberg
    Department of Oncology, Radiology and Clinical Immunology, Division of Clinical Immunology, Uppsala University Hospital, SE 75185 Uppsala, Sweden
    Cell Transplant 17:1305-13. 2008
    ..The APS facilitates the standardization of this complex procedure according to cGMP standards...
  15. ncbi Low molecular weight dextran sulfate prevents the instant blood-mediated inflammatory reaction induced by adult porcine islets
    Masafumi Goto
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, Uppsala, Sweden
    Transplantation 77:741-7. 2004
    ....
  16. ncbi Co-stimulatory molecules in islet xenotransplantation: CTLA4Ig treatment in CD40 ligand-deficient mice
    Birgitta Benda
    Section of Clinical Immunology, Department of Oncology, Radiology, and Clinical Immunology, The Rudbeck Laboratory, Uppsala University, SE 751 85 Uppsala, Sweden
    Cell Transplant 11:715-20. 2002
    ....
  17. ncbi Immunosuppressive drugs in islet xenotransplantation: a tool for gaining further insights in the mechanisms of the rejection process
    Henrik Krook
    Division of Clinical Immunology, Rudbeck Laboratory, Uppsala University, SE 751 85 Uppsala, Sweden
    Transplantation 74:1084-9. 2002
    ....
  18. ncbi Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model
    H Brandhorst
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Cell Transplant 22:1477-83. 2013
    ..7 vs. 39.3 ± 6.4%, p < 0.01) was measured in islets isolated from F6H8-preserved pancreata. The present data suggest that F6H8 does not increase islet yield but improves quality of pig islets isolated after prolonged cold ischemia...
  19. ncbi T cells in islet-like cell cluster xenograft rejection: a study in the pig-to-mouse model
    B Benda
    Department of Oncology, Radiology, and Clinical Immunology, Uppsala University, Sweden
    Transplantation 66:435-40. 1998
    ..The aim of the present study was to evaluate the nature of T cells involved in and, presumably, critical to fetal porcine islet-like cell cluster (ICC) xenograft rejection...
  20. ncbi Islet surface heparinization prevents the instant blood-mediated inflammatory reaction in islet transplantation
    Sanja Cabric
    Division of Clinical Immunology, Department of Oncology, Radiology, and Clinical Immunology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Diabetes 56:2008-15. 2007
    ..To avoid systemic treatment, the aim of the present study was to render the islet graft blood biocompatible by applying a continuous heparin coating to the islet surface...
  21. ncbi Intragraft cytokine mRNA expression in rejecting and non-rejecting vascularized xenografts
    Tomas Lorant
    Departments of Transplantation Surgery and Clinical Immunology, University Hospital, SE 751 85 Uppsala, Sweden
    Xenotransplantation 10:311-24. 2003
    ..A parallel investigation was made of the antibody response of IgM and IgG to the xenografts...
  22. ncbi Low-molecular weight dextran sulfate abrogates the instant blood-mediated inflammatory reaction induced by adult porcine islets both in vitro and in vivo
    M Goto
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplant Proc 36:1186-7. 2004
    ..As a consequence of IBMIR, morphological destruction of porcine islets occurs within the first few hours after transplantation...
  23. pmc Neutrophilic granulocytes are the predominant cell type infiltrating pancreatic islets in contact with ABO-compatible blood
    L Moberg
    Department of Oncology, Radiology and Clinical Immunology, Division of Clinical Immunology, Uppsala University Hospital, Sweden
    Clin Exp Immunol 142:125-31. 2005
    ..Because islets are known to be exquisitely susceptible to oxidative stress, development of drugs targeting neutrophilic cytotoxicity could markedly improve the outcome of islet transplantation...
  24. ncbi The transplanted fetal endocrine pancreas undergoes an inherent sequential differentiation similar to that in the native pancreas. An ultrastructural study in the pig-to-mouse model
    A Lukinius
    Department of Genetics and Pathology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Diabetes 50:962-71. 2001
    ..Therefore, the results presented strongly suggest that the ontogeny of the four major endocrine islet cells is determined by genetic information carried by the progenitor cells and not by the systemic or local environment...
  25. doi Positron emission tomography in clinical islet transplantation
    O Eriksson
    Department of Radiology, Oncology and Clinical Immunology, Division of Radiology, University Hospital, Uppsala, Sweden
    Am J Transplant 9:2816-24. 2009
    ..The technique has potential to be used to assess approaches to enhance islet survival and engraftment in clinical transplantation...
  26. ncbi Ex vivo propagation and characterization of lymphocytes from rejecting rat-kidney allografts
    M Engstrand
    Department of Clinical Immunology, University Hospital, Uppsala, Sweden
    Transpl Immunol 7:189-96. 1999
    ..This technique offers a possibility to study cellular reactivity in allotransplantation...
  27. ncbi Expression of islet amyloid polypeptide in fetal and adult porcine and human pancreatic islet cells
    A Lukinius
    Department of Pathology, University of Uppsala, Sweden
    Endocrinology 138:5319-25. 1997
    ..The substance is more widely distributed within the pancreatic islet cell types than are any of the other islet hormones, which presumably has functional implications...
  28. ncbi Intraportal pig islet xenotransplantation into athymic mice as an in vivo model for the study of the instant blood-mediated inflammatory reaction
    Masafumi Goto
    Department of Radiology, Oncology, and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Xenotransplantation 11:195-202. 2004
    ..Intraportal transplantation of APIs into athymic mice induced IBMIR, thus providing a small animal model for studying xenogeneic IBMIR...
  29. ncbi Lymphocyte propagation from biopsies of kidney allografts
    Mats Engstrand
    Department of Clinical Immunology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transpl Immunol 16:215-9. 2006
    ..Tissue culturing is easy to perform and evaluate and can be used to determine and analyse the cellular immune response to allografts and may thus be used as a complement to morphological analyses...
  30. pmc Obtaining regulatory T cells from uraemic patients awaiting kidney transplantation for use in clinical trials
    D Berglund
    Department of Surgical Sciences, Section of Transplantation Surgery, Uppsala University, Uppsala, Sweden
    Clin Exp Immunol 173:310-22. 2013
    ..This is a direct comparison between different isolation techniques and expansion protocols with T(regs) from uraemic patients that may guide future efforts to produce clinical-grade T(regs) for use in kidney transplantation...
  31. doi Cytomegalovirus-specific CD4 and CD8 T cell responses in infants and children
    A K Lidehall
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Scand J Immunol 77:135-43. 2013
    ..In conclusion, inadequate CD 4 T cell function seems to be involved in the failure to get immune control of the CMV infection in children younger than 2 years of age with congenital as well as postnatal CMV infection...
  32. doi New class of oxygen carriers improves islet isolation from long-term stored rat pancreata
    H Brandhorst
    Department of Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Transplant Proc 40:393-4. 2008
    ..33 g/cm3) and lipophilic qualities, to facilitate islet isolation from long-term stored rat pancreata...
  33. ncbi No beneficial effect of two-layer storage compared with UW-storage on human islet isolation and transplantation
    Jose Caballero-Corbalan
    Department of Oncology, Radiology and Clinical Immunology, Division of Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Transplantation 84:864-9. 2007
    ..The present study aimed to clarify whether TLM enhances the ischemic tolerance of human pancreata...
  34. ncbi Inhibition of thrombin abrogates the instant blood-mediated inflammatory reaction triggered by isolated human islets: possible application of the thrombin inhibitor melagatran in clinical islet transplantation
    Lisa Ozmen
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Diabetes 51:1779-84. 2002
    ..This protective effect indicates that thrombin plays a pivotal role in IBMIR and suggests that thrombin inhibition can improve the outcome of clinical islet transplantation...
  35. pmc Clinical and experimental pancreatic islet transplantation to striated muscle: establishment of a vascular system similar to that in native islets
    Gustaf Christoffersson
    Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
    Diabetes 59:2569-78. 2010
    ..The aim of the current study was to evaluate striated muscle as a potential angiogenic site for islet transplantation...
  36. doi Insulin-heparin infusions peritransplant substantially improve single-donor clinical islet transplant success
    Angela Koh
    1 Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada 2 Division of Clinical Immunology, Department of Oncology, Radiology and Clinical Immunology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Transplantation 89:465-71. 2010
    ..The ability to achieve insulin independence with a single donor is an important goal in clinical islet transplantation due to the limited organ supply...
  37. pmc Anchoring of vascular endothelial growth factor to surface-immobilized heparin on pancreatic islets: implications for stimulating islet angiogenesis
    Sanja Cabric
    Division of Clinical Immunology, Department of Oncology, Radiology, and Clinical Immunology, Uppsala University, Uppsala, Sweden
    Tissue Eng Part A 16:961-70. 2010
    ....
  38. doi CMV-specific T-cell immunity, viral load, and clinical outcome in seropositive renal transplant recipients: a pilot study
    Fredrik Sund
    Department of Infectious Diseases, University Hospital, SE 751 85 Uppsala, Sweden
    Clin Transplant 24:401-9. 2010
    ..The aim of this prospective study of renal transplant recipients was to evaluate the dynamics of CMV-specific T-cells, viral load, and clinical symptoms of CMV infection...
  39. ncbi Interleukin-6 in islet xenograft rejection
    B Benda
    Section of Clinical Immunology, Department of Oncology, Radiology and Clinical Immunology, The Rudbeck Laboratory, Uppsala University, 75185 Uppsala, Sweden
    Transpl Int 14:63-71. 2001
    ..In the pig-to-mouse model, IL-6 seems to be of minor importance to fetal porcine ICC xenograft rejection. Macrophages, and not eosinophils, dominate the cellular response associated with this process...
  40. ncbi Nicotinamide inhibits tissue factor expression in isolated human pancreatic islets: implications for clinical islet transplantation
    Lisa Moberg
    Department of Radiology, Oncology, and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplantation 76:1285-8. 2003
    ..In this study, the authors investigated whether compounds previously shown to affect TF and macrophage chemoattractant protein (MCP)-1 expression in monocytes and endothelial cells have the same effect in human islet cells...
  41. ncbi Adenovirus-mediated expression of human CD55 or CD59 protects adult porcine islets from complement-mediated cell lysis by human serum
    Peter Schmidt
    Department of Clinical Immunology, Uppsala University, Uppsala, Sweden
    Transplantation 75:697-702. 2003
    ..The functional capacity of the transgenes was examined in vitro after exposure to fresh human serum...
  42. ncbi MyD88-dependent toll-like receptor signalling is not a requirement for fetal islet xenograft rejection in mice
    Peter Schmidt
    Division of Clinical Immunology, The Rudbeck Laboratory, Uppsala University, Sweden
    Xenotransplantation 11:347-52. 2004
    ..These mice have been shown to have markedly impaired Th1 immunity...
  43. ncbi Positron emission tomography: a real-time tool to quantify early islet engraftment in a preclinical large animal model
    Torsten Eich
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, University Hospital, Uppsala, Sweden
    Transplantation 84:893-8. 2007
    ..Therefore, the objective of the present study was to develop a technique for imaging survival of transplanted islets in the peritransplant and early posttransplant phase...
  44. doi A new oxygen carrier for improved long-term storage of human pancreata before islet isolation
    Heide Brandhorst
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Transplantation 89:155-60. 2010
    ..The aim of this study was to compare PFD with a newly developed oxygen carrier composed of perfluorohexyloctane and polydimethylsiloxane 5 (F6H8S5) for long-term storage of human pancreata...
  45. doi The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas
    Heide Brandhorst
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Transplantation 85:456-61. 2008
    ....
  46. doi The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation
    Heide Brandhorst
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Transplantation 87:370-5. 2009
    ..Despite improved characterization of these components, the lot-related variability in efficacy still persists suggesting that additional so far disregarded enzymes are required for efficient islet cleavage...
  47. pmc Can cells and biomaterials in therapeutic medicine be shielded from innate immune recognition?
    Bo Nilsson
    Division of Clinical Immunology, Rudbeck Laboratory, Uppsala University, Sweden
    Trends Immunol 31:32-8. 2010
    ..We discuss here the new technologies that are being developed to shield the biomaterial and cell surfaces from recognition by the innate immune system...
  48. ncbi A new murine model of islet xenograft rejection: graft destruction is dependent on a major histocompatibility-specific interaction between T-cells and macrophages
    Peter Schmidt
    Division of Clinical Immunology, Uppsala University, Rudbeck Laboratory CII, Dag Hammerskjölds väg 20, SE 75185 Uppsala, Sweden
    Diabetes 52:1111-8. 2003
    ..Interaction between the TCR of transferred T-cells and MHC on host endothelial cells and/or macrophages seems necessary for inducing ICC xenograft rejection...
  49. doi Perfluorohexyloctane improves long-term storage of rat pancreata for subsequent islet isolation
    Heide Brandhorst
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Uppsala, Sweden
    Transpl Int 22:1017-22. 2009
    ..This proof-of-concept study demonstrated in rats that islet isolation performed after long-term storage of oxygenated pancreatic tissue can be significantly improved if PFD was replaced by F6H8...
  50. ncbi Inflammatory mediators expressed in human islets of Langerhans: implications for islet transplantation
    Ulrika Johansson
    Department of Clinical Immunology, The Rudbeck Laboratory, Uppsala University C11, Sweden
    Biochem Biophys Res Commun 308:474-9. 2003
    ..In conclusion, several inflammatory mediators were expressed in isolated islets, particularly at an early stage after isolation, indicating that a few days of culture could be beneficial for the outcome of islet transplantation...
  51. pmc A new method for incorporating functional heparin onto the surface of islets of Langerhans
    Sanja Cabric
    Department of Oncology, Radiology, and Clinical Immunology, Uppsala University, Uppsala, Sweden
    Tissue Eng Part C Methods 14:141-7. 2008
    ..The technique presented allows human islets of Langerhans to successfully be covered with functional heparin as a means to reduce instant blood-mediated inflammatory reactions induced by the innate immune system...
  52. ncbi Quantification of lymphocytes propagating from rat-kidney allografts--a tool to monitor anti-rejection treatment
    Mats Engstrand
    Transpl Immunol 10:31-6. 2002
    ..The objective of the present study was to develop a diagnostic tool enabling rapid assessment of the effects of an attempted anti-rejection treatment...
  53. ncbi Adenovirus-mediated expression of the anticoagulant hirudin in human islets: a tool to make the islets biocompatible to blood
    Sanja Cabric
    Division of Clinical Immunology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Cell Transplant 15:759-67. 2006
    ..The impairment in glucose-stimulated insulin release in hirudin-secreting islets, compared to controls, indicates that the additional protein synthesis affects the functional capacity of the islets...
  54. ncbi Tissue factor produced by the endocrine cells of the islets of Langerhans is associated with a negative outcome of clinical islet transplantation
    Helena Johansson
    The Rudbeck Laboratory, University Hospital, 751 85 Uppsala, Sweden
    Diabetes 54:1755-62. 2005
    ..In conclusion, tissue factor present in both the islets and the culture medium and elicits IBMIR, which affects the function of the transplanted islets...
  55. ncbi Possible transmission of zoonoses in xenotransplantation: porcine endogenous retroviruses (PERVs) from an immunological point of view
    Peter Schmidt
    The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Acta Vet Scand Suppl 99:27-34. 2004
  56. ncbi T cell control of primary and latent cytomegalovirus infections in healthy subjects
    Anna K Lidehall
    Divisions of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden
    J Clin Immunol 25:473-81. 2005
    ..In conclusion, a majority of healthy individuals readily seem to control latent CMV infection, whereas a subpopulation (1/3) of individuals uses a large proportion of their CD8+ T cell repertoire to control the infection...
  57. ncbi Inflammatory gene expression in Coxsackievirus B-4-infected human islets of Langerhans
    Annika Olsson
    Division of Clinical Immunology, Uppsala University, Uppsala, Sweden
    Biochem Biophys Res Commun 330:571-6. 2005
    ..These cytokines and chemokines have the potential to rapidly induce inflammatory reactions when expressed in vivo and could contribute to the autoimmune reactions associated with the development of T1DM...
  58. ncbi Pig islet xenotransplantation: activation of porcine endogenous retrovirus in the immediate post-transplantation period
    Peter Schmidt
    The Rudbeck Laboratory, Division of Clinical Immunology, Uppsala University, Uppsala, Sweden
    Xenotransplantation 12:450-6. 2005
    ..In vivo activation of PERV have so far not been examined. Expression of PERV was examined in adult and fetal porcine islets with or without the presence of known retroviral inducers or after transplantation to rats...
  59. pmc Formation of composite endothelial cell-mesenchymal stem cell islets: a novel approach to promote islet revascularization
    Ulrika Johansson
    Division of Clinical Immunology, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Uppsala, Sweden
    Diabetes 57:2393-401. 2008
    ..MSCs were added to our previously described composite EC islets as a potential means to improve their capacity for islet angiogenesis...
  60. ncbi Composite islet-endothelial cell grafts: a novel approach to counteract innate immunity in islet transplantation
    Ulrika Johansson
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, Uppsala University, Sweden
    Am J Transplant 5:2632-9. 2005
    ....
  61. doi Degraded collagenase deteriorates islet viability
    H Brandhorst
    Department of Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden
    Transplant Proc 40:370-1. 2008
    ..The present study sought to assess the effect of degraded collagenase on islet function in vitro and posttransplantation...
  62. ncbi Induction of the chemokine interferon-gamma-inducible protein-10 in human pancreatic islets during enterovirus infection
    A K Berg
    Department of Women s and Children s Health, Uppsala University, Akademiska sjukhuset, ing 95 96, SE 751 85 Uppsala, Sweden
    Diabetologia 49:2697-703. 2006
    ....
  63. ncbi Refinement of the automated method for human islet isolation and presentation of a closed system for in vitro islet culture
    Masafumi Goto
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, University Hospital, Uppsala, Sweden
    Transplantation 78:1367-75. 2004
    ..The isolation protocol has also been modified to meet current GMP (cGMP) standards. Moreover, the impact of donor- and process-related factors was correlated to the use of islets for clinical transplantation...
  64. ncbi Effects on isolated human pancreatic islet cells after infection with strains of enterovirus isolated at clinical presentation of type 1 diabetes
    Asma Elshebani
    Department of Women s and Children s Health, University Hospital, Uppsala University, Uppsala, Sweden
    Virus Res 124:193-203. 2007
    ..In addition, the infection caused a clear increase in the number of dead cells...
  65. ncbi Antiviral treatment of Coxsackie B virus infection in human pancreatic islets
    Anna Karin Berg
    Department of Women s and Children s Health, Uppsala University, Akademiska Hospital, ing 95 96 nbv, SE 751 85 Uppsala, Sweden
    Antiviral Res 74:65-71. 2007
    ..However, genetic differences between these strains influenced the effectiveness of pleconaril treatment. This stresses the importance of using multiple viral strains in antiviral tests...
  66. pmc Tropism Analysis of Two Coxsackie B5 Strains Reveals Virus Growth in Human Primary Pancreatic Islets but not in Exocrine Cell Clusters In Vitro
    M Hodik
    Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Open Virol J 7:49-56. 2013
    ..The association of virus with insulin granules might reflect the use of these as replication scaffolds...
  67. ncbi Islet allo- and xenotransplantation in normal, intercellular adhesion molecule-1-deficient, and P selectin-deficient mice
    J O Sandberg
    Department of Clinical Immunology and Transfusion Medicine, Uppsala University, Sweden
    Transplantation 64:584-9. 1997
    ..These deficient mice provide powerful tools to study the complex role of cell adhesion molecules in the cellular interactions between graft and recipient that culminate in graft rejection...
  68. ncbi Technical improvement of human pancreatic islet isolation
    M Goto
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Transplant Proc 37:1313-4. 2005
    ..To improve this procedure, we introduced novel techniques to identify and repair tissue damage resulting in leakage of collagenase solution...
  69. doi Efficient adenovector CD40 ligand immunotherapy of canine malignant melanoma
    Henrik von Euler
    Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Center of Clinical Comparative Oncology C3O, Swedish University of Agricultural Sciences SLU, Uppsala, Sweden
    J Immunother 31:377-84. 2008
    ..Our results indicate that AdCD40L immunogene therapy is beneficial in canine malignant melanoma and could be considered for human malignant melanoma as well...
  70. pmc Acute antibody-mediated complement activation mediates lysis of pancreatic islets cells and may cause tissue loss in clinical islet transplantation
    Jenny Tjernberg
    Department of Oncology, Rudbeck Laboratory, Uppsala, Sweden
    Transplantation 85:1193-9. 2008
    ..Complement activation is an important cytotoxic component of the IBMIR, and in the present study, we have investigated this component in detail...
  71. ncbi The recent finding that tissue factor is produced by the pancreatic islets constitutes a possible link between insulin resistance and cardiovascular disease
    Bo Nilsson
    Department of Radiology, Oncology, and Clinical Immunology, Division of Clinical Immunology, University Hospital, Uppsala, Sweden
    Am J Ther 12:551-4. 2005
    ..Here we review the field and set forth a hypothesis that links TF expression in the islets of Langerhans to the increased risk of CHD...
  72. ncbi Potent antitumor effects of CD154 transduced tumor cells in experimental bladder cancer
    A Loskog
    Clinical Immunology Division, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    J Urol 166:1093-7. 2001
    ..CONCLUSIONS: Our experimental results suggest that the potent antitumor effects of CD154 gene transduction should be considered for immunostimulatory gene therapy for bladder cancer...
  73. ncbi Reinnervation of syngeneic pancreatico-duodenal grafts in rats
    O Korsgren
    Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Sweden
    Transplantation 71:8-13. 2001
    ..CONCLUSIONS: The data suggest that the transplanted rat pancreas becomes reinnervated by mainly sympathetic nerve fibers...
  74. pmc Cellular responses to cytomegalovirus in immunosuppressed patients: circulating CD8+ T cells recognizing CMVpp65 are present but display functional impairment
    M Engstrand
    Division of Clinical Immunology, University Hospital, SE 751 85, Uppsala, Sweden
    Clin Exp Immunol 132:96-104. 2003
    ..Hence, these findings reflect impaired activation of cytotoxic T cells controlling latent CMV infection in immunosuppressed patients...
  75. doi Antiviral effect of nicotinamide on enterovirus-infected human islets in vitro: effect on virus replication and chemokine secretion
    Annika Moëll
    Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, The Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    J Med Virol 81:1082-7. 2009
    ..Also, this study highlights a possible mechanism of virus-induced type 1 diabetes through the induction of MCP-1 and IP-10 in pancreatic islets...
  76. ncbi Antibody-based proteomics for discovery and exploration of proteins expressed in pancreatic islets
    Cecilia Lindskog
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Discov Med 9:565-78. 2010
    ..One of these proteins, tetraspanin-7, is expressed on the cell membrane and could thus be a potential candidate for future development of tracers for beta cell imaging...
  77. ncbi Adenovirus CD40 ligand gene therapy counteracts immune escape mechanisms in the tumor Microenvironment
    Angelica Loskog
    Clinical Immunology Division, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    J Immunol 172:7200-5. 2004
    ..Our results argue for using adenovirus CD40L gene transfer, alone or in combination with other modalities, for the treatment of Th2-dominated tumors...
  78. doi In vivo and in vitro characterization of [18F]-FE-(+)-DTBZ as a tracer for beta-cell mass
    Olof Eriksson
    Division of Radiology, Department of Oncology, Radiology and Clinical Immunology, University Hospital, Uppsala, Sweden
    Nucl Med Biol 37:357-63. 2010
    ..The purpose was to investigate in vitro and in vivo utility of this tracer for the assessment of beta-cell mass...
  79. ncbi [Replacement therapy with beta-cells: the way to treat diabetes in the future?]
    Timo Otonkoski
    Biomedicum Helsinki, kehitys ja lisääntymisbiologian ohjelma PL 700, 00029 HUS
    Duodecim 120:1133-4. 2004
  80. ncbi Maturation of in vitro-generated human islets after transplantation in nude mice
    Ru Gao
    Program of Developmental and Reproductive Biology, Biomedicum Helsinki, University of Helsinki, Finland
    Mol Cell Endocrinol 264:28-34. 2007
    ..Ductal cell differentiation into endocrine cells may be an important factor in sustaining the long-term function of islet transplants...
  81. ncbi Interferons induce an antiviral state in human pancreatic islet cells
    Monica Hultcrantz
    Center for Infectious Medicine F59, Department of Medicine, Karolinska Institutet, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Virology 367:92-101. 2007
    ..This may be critical for beta cell survival and protection from virus-induced T1D in humans...
  82. ncbi The efficacy of CD40 ligand blockade in discordant pig-to-rat islet xenotransplantation is correlated with an immunosuppressive effect of immunoglobulin
    Lars Wennberg
    Karolinska Institute, Department of Transplantation Surgery, Karolinska University Hospital, Huddinge, Sweden
    Transplantation 79:157-64. 2005
    ..The authors' aim was to evaluate the efficacy of immunosuppression with monoclonal anti-CD40 ligand antibodies (aCD40L) or nonspecific polyclonal intravenous immunoglobulin (IVIG) in the pig-to-rat islet xenotransplantation model...
  83. doi Beta-cell transplantation for diabetes therapy
    Camillo Ricordi
    Lancet 372:27-8; author reply 29-30. 2008
  84. ncbi Effect of plasma exchange in combination with deoxyspergualin on the survival of guinea-pig hearts in macrophage-depleted C6-deficient rats
    Guosheng Wu
    Department of Transplantation Surgery, Huddinge Hospital, Karolinska Institutet, Stockholm, Sweden
    Xenotransplantation 10:214-22. 2003
    ..These findings suggest that strategies targeting antibody and macrophages may be useful in prolonging xenograft survival...
  85. ncbi Characterization of endocrine progenitor cells and critical factors for their differentiation in human adult pancreatic cell culture
    Ru Gao
    Program of Developmental and Reproductive Biology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland
    Diabetes 52:2007-15. 2003
    ..Our results support the previous notion that pancreatic progenitor cells represent a subpopulation of ductal epithelial cells. No evidence was found for the development of endocrine cells from nestin-positive stem cells...
  86. doi Glucocorticoids reduce pro-inflammatory cytokines and tissue factor in vitro and improve function of transplanted human islets in vivo
    Tormod Lund
    Surgical Clinic, Section for Transplantation, Rikshospitalet University Hospital, Oslo, Norway
    Transpl Int 21:669-78. 2008
    ..In conclusion, glucocorticoids have potent anti-inflammatory properties on human islets without permanent effects on insulin metabolism. Brief glucocorticoid exposure improves function of transplanted human islets in vivo...
  87. doi Widespread amyloid deposition in transplanted human pancreatic islets
    Gunilla T Westermark
    N Engl J Med 359:977-9. 2008
  88. doi Beta-cell transplantation for diabetes therapy
    Antonio Secchi
    Lancet 372:28-9; author reply 29-30. 2008
  89. ncbi Intracerebral cytokine profiles in adult rats grafted with neural tissue of different immunological disparity
    Bilal Mirza
    Section for Neuronal Survival, Department of Physiological Sciences and Neuroscience, Wallenberg Neuroscience Center, Lund University, BMC A10, 221 84 Lund, Sweden
    Brain Res Bull 63:105-18. 2004
    ..In allografts, a chronic inflammatory reaction was detected and in xenogeneic grafts a delayed hypersensitivity like reaction may be involved in rejection...
  90. doi Low molecular weight dextran sulfate as complement inhibitor and cytoprotectant in solid organ and islet transplantation
    Rolf Spirig
    Department of Clinical Research, University of Bern, Switzerland
    Mol Immunol 45:4084-94. 2008
    ..Taken together the available data suggest that DXS may be a useful reagent to prevent the activation of innate immunity, both in solid organ and islet transplantation...

Research Grants2

  1. Innate immunity in clinical islet transplantation
    Olle Korsgren; Fiscal Year: 2007
    ..The successful completion of the clinical studies proposed will form a foundation for subsequent clinical trials aiming for induction of immunological tolerance. ..