Research Topics
Species | Jonas JarviusSummaryAffiliation: Uppsala University Country: Sweden Publications
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Detail Information
Publications
DNA Skyline: fonts to facilitate visual inspection of nucleic acid sequencesJonas Jarvius
Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden
Biotechniques 40:740. 2006
A dual-tag microarray platform for high-performance nucleic acid and protein analysesOlle Ericsson
Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, SE 75185 Uppsala, Sweden
Nucleic Acids Res 36:e45. 2008..We provide proof of concept for microarray-based measurement of both mRNA molecules and of proteins, converted to tag DNA sequences by padlock and proximity probe ligation, respectively...- Prospects for in situ analyses of individual and complexes of DNA, RNA, and protein molecules with padlock and proximity probesUlf Landegren
Department of Genetics and Pathology, University of Uppsala, S-751-85 Uppsala, Sweden
Methods Cell Biol 75:787-97. 2004 - Proximity ligation assays for sensitive and specific protein analysesSigrun M Gustafsdottir
Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, SE-75185 Uppsala, Sweden
Anal Biochem 345:2-9. 2005 - A sense of closeness: protein detection by proximity ligationMats Gullberg
The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden
Curr Opin Biotechnol 14:82-6. 2003..Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction... - Molecular tools for a molecular medicine: analyzing genes, transcripts and proteins using padlock and proximity probesUlf Landegren
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE 751 85 Uppsala, Sweden
J Mol Recognit 17:194-7. 2004..The procedures permit highly specific solution-phase or localized analyses of large sets of target molecules as required in future molecular analyses... 
Ligation-based molecular tools for lab-on-a-chip devicesJonas Melin
Department of Genetics and Pathology, Rudbeck Laboratory, University of Uppsala, SE 75185 Uppsala, Sweden
N Biotechnol 25:42-8. 2008..We discuss these problems and describe a series of molecular tools that may present new opportunities for lab-on-a-chip devices at the point-of-care...- Cytokine detection by antibody-based proximity ligationMats Gullberg
The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden
Proc Natl Acad Sci U S A 101:8420-4. 2004..The technique is applied to measure cytokines in a homogenous test format with femtomolar detection sensitivities in 1-microl samples, and we exemplify its utility in situations when only minute sample amounts are available... - Proximity ligation: a specific and versatile tool for the proteomic eraOla Söderberg
University of Uppsala, Department of Genetics and Pathology, Rudbeck Laboratory, SE 751 85 Uppsala, Sweden
Genet Eng (N Y) 28:85-93. 2007..In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique... - High content screening for inhibitors of protein interactions and post-translational modifications in primary cells by proximity ligationKarl Johan Leuchowius
Molecular Medicine, Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, Dag Hammarskjölds väg 20, S 751 85 Uppsala, Sweden
Mol Cell Proteomics 9:178-83. 2010..In addition, inhibitor-induced perturbation of protein-protein interactions of the PDGF signaling pathway could be quantified, further demonstrating the usefulness of the assay in drug discovery... - Digital quantification using amplified single-molecule detectionJonas Jarvius
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden
Nat Methods 3:725-7. 2006..We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae... - Homogeneous amplified single-molecule detection: Characterization of key parametersJonas Melin
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, S 751 85 Uppsala, Sweden
Anal Biochem 368:230-8. 2007..Therefore, the signal/noise ratio is limited by the number of detection sites. By increasing the density of detection sites in the RCP by a factor of 1.9, we show that the optical signal/noise level can be increased from 42 to 75... - A single molecule array for digital targeted molecular analysesJenny Göransson
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
Nucleic Acids Res 37:e7. 2009..The decoding strategy is generic in that the target can be any biomolecule which has been encoded into a DNA circle via a molecular probing reaction... - Direct observation of individual endogenous protein complexes in situ by proximity ligationOla Söderberg
Department of Genetics and Pathology, Rudbeck Laboratory, University of Uppsala, SE 75185 Uppsala, Sweden
Nat Methods 3:995-1000. 2006..We used this method to show specific regulation of protein-protein interactions between endogenous Myc and Max oncogenic transcription factors in response to interferon-gamma (IFN-gamma) signaling and low-molecular-weight inhibitors... - Thermoplastic microfluidic platform for single-molecule detection, cell culture, and actuationJonas Melin
Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Angstrom Laboratory, University of Uppsala, Sweden
Anal Chem 77:7122-30. 2005..Biocompatibility of the platform was illustrated by culturing Chinese hamster ovary cells for 7 days in the microfluidic channels... - Parallel gene analysis with allele-specific padlock probes and tag microarraysJohan Baner
The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE 751 85 Uppsala, Sweden
Nucleic Acids Res 31:e103. 2003..The technique was illustrated by analyzing normal and pathogenic variation within the Wilson disease-related ATP7B gene, both at the level of DNA and RNA, using allele-specific padlock probes... - Oligonucleotide ligation assayJonas Jarvius
Rudbeck Laboratory, Unit of Molecular Medicine, Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden
Methods Mol Biol 212:215-28. 2003 - Endothelial cell migration in stable gradients of vascular endothelial growth factor A and fibroblast growth factor 2: effects on chemotaxis and chemokinesisIrmeli Barkefors
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
J Biol Chem 283:13905-12. 2008..Our findings suggest that chemotactic growth factor gradients may instruct endothelial cells to shift toward a nonmigratory phenotype when approaching the growth factor source... - Protein detection using proximity-dependent DNA ligation assaysSimon Fredriksson
The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE-75185 Uppsala, Sweden
Nat Biotechnol 20:473-7. 2002..This proximity ligation assay detects zeptomole (40 x 10(-21) mol) amounts of the cytokine platelet-derived growth factor (PDGF) without washes or separations, and the mechanism can be generalized to other forms of protein analysis...