Research Topics
Genomes and GenesSpecies | Björn HerrmannSummaryAffiliation: Uppsala University Country: Sweden Publications
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Detail Information
Publications
Prevalence trends of the new variant of Chlamydia trachomatis in four counties of Sweden in 2007-2011Björn Herrmann
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
Sex Transm Dis 39:648-50. 2012..Future studies are needed to see if nvCT will decline further...
Emergence and spread of Chlamydia trachomatis variant, SwedenBjörn Herrmann
Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden
Emerg Infect Dis 14:1462-5. 2008..In a nationwide study, we found that it is now prevalent across Sweden, irrespective of the detection system used. Genetic analysis by multilocus sequence typing identified a predominant variant, suggesting recent emergence...
[We need a better strategy against Chlamydia infections]Björn Herrmann
Lakartidningen 103:2155; discussion 2156. 2006- [New Chlamydia variation discovered in 2006. Quick transmission in Sweden, diagnostic techniques missed the mutation]Björn Herrmann
sektionen för klinisk mikrobiologi, Akademiska sjukhuset, Uppsala
Lakartidningen 104:3535-6. 2007 - Comparison of a duplex quantitative real-time PCR assay and the COBAS Amplicor CMV Monitor test for detection of cytomegalovirusBjörn Herrmann
Section of Virology, Department of Medical Sciences, Uppsala University, Sweden
J Clin Microbiol 42:1909-14. 2004..In conclusion, a duplex qPCR assay in a real-time format facilitates quantitative measurements and minimizes the risk of false-negative results... - [Chlamydia infections increasing in Sweden, too. Better knowledge and a national intervention program are necessary]Björn Herrmann
Avdelningen för klinisk mikrobiologi, Akademiska sjukhuset, Uppsala
Lakartidningen 103:1412-5. 2006 - Simultaneous detection and typing of influenza viruses A and B by a nested reverse transcription-PCR: comparison to virus isolation and antigen detection by immunofluorescence and optical immunoassay (FLU OIA)B Herrmann
Section of Virology, Department of Clinical Microbiology, University Hospital, S 751 85 Uppsala, Sweden
J Clin Microbiol 39:134-8. 2001..The RT-PCR provides a sensitive and specific method for detecting and typing influenza viruses A and B. The rapid OIA is useful as a complementary test, but it cannot replace established methods without further evaluation... - Chlamydophila abortus in a Brown skua (Catharacta antarctica lonnbergi) from a subantarctic islandB Herrmann
Section of Virology, Department of Clinical Microbiology, University Hospital, S 751 85 Uppsala, Sweden
Appl Environ Microbiol 66:3654-6. 2000..Phylogenetic analysis of the rnpB and omp1 genes indicated the strain to be more closely related to C. abortus than to 6BC, the type strain of Chlamydophila psittaci... - Characterization of the rnpB gene and RNase P RNA in the order ChlamydialesB Herrmann
Department of Clinical Microbiology, University Hospital, Uppsala, Sweden
Int J Syst Evol Microbiol 50:149-58. 2000..It was also demonstrated that the RNase P RNA derived from Chlamydia trachomatis is able to cleave a tRNA precursor in the absence of protein. These findings are discussed in relation to the structure of Chlamydia RNase P RNA... - Characterization of ompA genotypes by sequence analysis of DNA from all detected cases of Chlamydia trachomatis infections during 1 year of contact tracing in a Swedish CountyMaria Lysén
Section of Clinical Bacteriology, Department of Medical Sciences, University of Uppsala, Sweden
J Clin Microbiol 42:1641-7. 2004..Clinical manifestations were not associated with genotypes. Sequence variation was linked to sexual networks identified by contact tracing and improved epidemiological knowledge but was of limited benefit... - Chlamydia psittaci in Swedish wetland birds: a risk to zoonotic infection?Maria Blomqvist
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, S 751 85 Uppsala, Sweden
Avian Dis 56:737-40. 2012..Considering exposure to humans it is concluded that the risk of zoonotic infection is low... - Detection of Haemophilus influenzae in respiratory secretions from pneumonia patients by quantitative real-time polymerase chain reactionGuma M K Abdeldaim
Department of Clinical Microbiology, Uppsala University Hospital, S 751 85 Uppsala, Sweden
Diagn Microbiol Infect Dis 64:366-73. 2009..influenzae in respiratory secretions. Quantification facilitates discrimination between disease-causing H. influenzae strains and commensal colonization... - Multilocus sequence typing of urogenital Chlamydia trachomatis from patients with different degrees of clinical symptomsLinus Christerson
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
Sex Transm Dis 38:490-4. 2011..In the past, contradictory results have been obtained linking Chlamydia trachomatis serovars (ompA gene) to different clinical courses of infection... - Chlamydia trachomatis strains show specific clustering for men who have sex with men compared to heterosexual populations in Sweden, the Netherlands, and the United StatesLinus Christerson
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
J Clin Microbiol 50:3548-55. 2012..Our study supports the hypotheses of both tissue tropism as well as epidemiological network structures as explanations for the linkage between specific genetic variants and sexual orientation... - Phylogenetic relationships and genotyping of the genus Streptococcus by sequence determination of the RNase P RNA gene, rnpBJenny Täpp
Department of Clinical Microbiology, University Hospital, SE 751 85 Uppsala, Sweden
Int J Syst Evol Microbiol 53:1861-71. 2003..It can be concluded that the rnpB gene is suitable for phylogenetic analysis of closely related taxa and has potential as a tool for species discrimination... - Variation-tolerant capture and multiplex detection of nucleic acids: application to detection of microbesChristina Ohrmalm
Section of Clinical Virology, Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden
J Clin Microbiol 50:3208-15. 2012..It can be run on an ordinary PCR and a Luminex machine and is suitable for both clinical diagnoses and microbial surveillance... - Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidisGuma M K Abdeldaim
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
BMC Microbiol 10:310. 2010..The method was evaluated on bronchoalveolar lavage (BAL) samples from 156 adults with lower respiratory tract infection (LRTI) and 31 controls, and on 87 cerebrospinal fluid (CSF) samples from meningitis patients... - Mosaic structure of intragenic repetitive elements in histone H1-like protein Hc2 varies within serovars of Chlamydia trachomatisMarkus Klint
Department of Clinical Microbiology, Uppsala University, Uppsala, Sweden
BMC Microbiol 10:81. 2010..A more complex structure was seen in this study when sequences from 378 specimens covering the hctB gene, which encodes Hc2, were compared... - Differentiation of Chlamydia spp. by sequence determination and restriction endonuclease cleavage of RNase P RNA genesB Herrmann
Department of Clinical Microbiology, University Hospital, Uppsala, Sweden
J Clin Microbiol 34:1897-902. 1996..This structure has not previously been found among RNase P RNAs of members of the division Bacteria... - Broadly targeted triplex real-time PCR detection of influenza A, B and C viruses based on the nucleoprotein gene and a novel "MegaBeacon" probe strategyShaman Muradrasoli
Section of Clinical Virology, Department of Medical Sciences, Uppsala University, Sweden
J Virol Methods 163:313-22. 2010..MegaBeacon probes hold promise for variable target nucleic acids... - Chlamydophila psittaci in Fulmars, the Faroe IslandsBjörn Herrmann
University Hospital, Uppsala, Sweden
Emerg Infect Dis 12:330-2. 2006..Analysis of ompA showed a sequence almost identical to that of the type strain. The origin of C. psittaci outbreaks in fulmars is discussed. Despite a high level of exposure, the risk for transmission of C. psittaci to humans is low... - Toward a quantitative DNA-based definition of pneumococcal pneumonia: a comparison of Streptococcus pneumoniae target genes, with special reference to the Spn9802 fragmentGuma M K Abdeldaim
Department of Clinical Microbiology, Uppsala University Hospital, Uppsala S 751 85, Sweden
Diagn Microbiol Infect Dis 60:143-50. 2008..In conclusion, Spn9802 real-time PCR is highly sensitive and specific. The quantification it provides enables differentiation between pneumococcal pathogenicity and commensalism... - PCR detection of Haemophilus influenzae from respiratory specimensGuma M K Abdeldaim
Department of Clinical Microbiology, Uppsala, University Hospital, Uppsala, Sweden
Methods Mol Biol 943:115-23. 2013..Here we describe a quantitative real-time PCR assay based on the outer membrane P6 gene omp6, to detect H. influenzae and its application on respiratory tract specimens... - High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysisMarkus Klint
Department of Clinical Microbiology, Uppsala University Hospital, SE 751 85 Uppsala, Sweden
J Clin Microbiol 45:1410-4. 2007..To conclude, our MLST system can be used to discriminate C. trachomatis strains and can be applied to high-resolution molecular epidemiology... - Typing of lymphogranuloma venereum Chlamydia trachomatis strainsLinus Christerson
Uppsala University, Uppsala, Sweden
Emerg Infect Dis 16:1777-9. 2010..Specimens from an outbreak in 2003 in Europe were monoclonal. In contrast, several strains were in the United States in the 1980s, including a variant from Europe... - High-resolution genotyping of Chlamydia trachomatis by use of a novel multilocus typing DNA microarrayLinus Christerson
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, S 751 85 Uppsala, Sweden
J Clin Microbiol 49:2838-43. 2011..Moreover, testing can be completed within 1 working day rather than 3 or 4 days, with data analysis not requiring highly specialized personnel. The present MLT array represents a powerful alternative in C. trachomatis genotyping... - Usefulness of real-time PCR for lytA, ply, and Spn9802 on plasma samples for the diagnosis of pneumococcal pneumoniaG Abdeldaim
Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden
Clin Microbiol Infect 16:1135-41. 2010.... - Guidelines for high-resolution genotyping of Chlamydia trachomatis using multilocus sequence analysisLinus Christerson
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
Methods Mol Biol 903:51-64. 2012..It is based on five highly variable but stable target regions which are PCR amplified and DNA sequenced... - Duplex real-time polymerase chain reaction assay for detection and quantification of herpes simplex virus type 1 and herpes simplex virus type 2 in genital and cutaneous lesionsFinn Filén
Section of Dermatology and Venereology, Department of Medical Sciences, University of Uppsala, Uppsala, Sweden
Sex Transm Dis 31:331-6. 2004..A sensitive and specific method for detecting herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) is important for diagnosing genital and cutaneous infections... - Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in bloodAsa Innings
Department of Clinical Microbiology, Uppsala University Hospital, SE 751 85 Uppsala, Sweden
J Clin Microbiol 45:874-80. 2007..The assay was applied to 20 blood samples from nine patients and showed a sensitivity similar to that of culture... - Multiplex and quantifiable detection of nucleic acid from pathogenic fungi using padlock probes, generic real time PCR and specific suspension array readoutRonnie Eriksson
Uppsala University, Department of Medical Sciences, Sections of Clinical Virology and Clinical Bacteriology, Sweden
J Microbiol Methods 78:195-202. 2009..The method correctly detected fungi in a variety of clinical samples and offered quantitative information on fungal nucleic acid... - Is quantitative PCR for the pneumolysin (ply) gene useful for detection of pneumococcal lower respiratory tract infection?G Abdeldaim
Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden
Clin Microbiol Infect 15:565-70. 2009..However, as a high cut-off resulted in low sensitivity, quantitative ply-based PCR does not appear to be clinically useful. Quantitative PCR methods for S. pneumoniae using alternative gene targets should be evaluated... - Experiences with the new genetic variant of Chlamydia trachomatis in Orebro county, Sweden - proportion, characteristics and effective diagnostic solution in an emergent situationM Unemo
Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Euro Surveill 12:E5-6. 2007..LightMix 480HT seemed sensitive, specific, and enabled high throughput diagnostics. However, rare low positive samples may be false-negative. Frequent surveillance and evaluations of diagnostic methods worldwide are crucial... - Detection of Neisseria gonorrhoeae from air-dried genital samples by single-tube nested PCRB Herrmann
Departments of Clinical Microbiology, University Hospital, Uppsala, Sweden
J Clin Microbiol 34:2548-51. 1996..This sensitive nested PCR assay, combined with air-dried storage, allows for the detection of gonococci when specimen storage and transport times are extended and freezing conditions are not available...