M Ehrenberg

Summary

Affiliation: Uppsala University
Country: Sweden

Publications

  1. doi request reprint Medium-dependent control of the bacterial growth rate
    Mans Ehrenberg
    Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, S 751 24 Uppsala, Sweden
    Biochimie 95:643-58. 2013
  2. pmc Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs
    Andrey V Zavialov
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, SE 75124 Uppsala, Sweden
    J Biol 4:9. 2005
  3. doi request reprint Maximum rrn promoter activity in Escherichia coli at saturating concentrations of free RNA polymerase
    M Ehrenberg
    Department of Cell and Molecular Biology, Uppsala University, Sweden
    Biochimie 92:12-20. 2010
  4. ncbi request reprint Systems biology is taking off
    Mans Ehrenberg
    Department of Cell and Molecular Biology, Uppsala University, BMC, 751 24 Uppsala, Sweden
    Genome Res 13:2377-80. 2003
  5. pmc Hypothesis: hypersensitive plasmid copy number control for ColE1
    M Ehrenberg
    Department of Molecular Biology, Uppsala University, Sweden
    Biophys J 70:135-45. 1996
  6. ncbi request reprint Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site
    M Y Pavlov
    Department of Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 273:389-401. 1997
  7. ncbi request reprint A direct estimation of the context effect on the efficiency of termination
    M Y Pavlov
    Department of Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 284:579-90. 1998
  8. ncbi request reprint Novel roles for classical factors at the interface between translation termination and initiation
    R Karimi
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala, Sweden
    Mol Cell 3:601-9. 1999
  9. pmc Dissociation rates of peptidyl-tRNA from the P-site of E.coli ribosomes
    R Karimi
    Department of Molecular Biology, BMC, Box 590, S 751 24, Sweden
    EMBO J 15:1149-54. 1996
  10. ncbi request reprint Shutdown in protein synthesis due to the expression of mini-genes in bacteria
    V Dincbas
    Department of Cell and Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 291:745-59. 1999

Research Grants

Detail Information

Publications28

  1. doi request reprint Medium-dependent control of the bacterial growth rate
    Mans Ehrenberg
    Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, S 751 24 Uppsala, Sweden
    Biochimie 95:643-58. 2013
    ..Our analysis indicates that this is true only in special cases and that, in general, medium adaptation also depends on factors other than ppGpp...
  2. pmc Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs
    Andrey V Zavialov
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, SE 75124 Uppsala, Sweden
    J Biol 4:9. 2005
    ..In both the classical and motor protein models, GDP-to-GTP exchange is assumed to occur spontaneously on 'free' EF-G even in the absence of a guanine-nucleotide exchange factor (GEF)...
  3. doi request reprint Maximum rrn promoter activity in Escherichia coli at saturating concentrations of free RNA polymerase
    M Ehrenberg
    Department of Cell and Molecular Biology, Uppsala University, Sweden
    Biochimie 92:12-20. 2010
    ..56 s. This corresponds to a promoter activity of 107 transcripts/min and is assumed to be close to the V(max) of rrn P1 promoters...
  4. ncbi request reprint Systems biology is taking off
    Mans Ehrenberg
    Department of Cell and Molecular Biology, Uppsala University, BMC, 751 24 Uppsala, Sweden
    Genome Res 13:2377-80. 2003
  5. pmc Hypothesis: hypersensitive plasmid copy number control for ColE1
    M Ehrenberg
    Department of Molecular Biology, Uppsala University, Sweden
    Biophys J 70:135-45. 1996
    ....
  6. ncbi request reprint Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site
    M Y Pavlov
    Department of Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 273:389-401. 1997
    ..The overproduction of RF1 in cells deficient in RRF or lacking RF3 has effects on growth rate predicted by the in vitro experiments...
  7. ncbi request reprint A direct estimation of the context effect on the efficiency of termination
    M Y Pavlov
    Department of Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 284:579-90. 1998
    ..It is also striking that the affinity of ribosomes for a chromosomally expressed RF2 is at least three times higher than for RF2 isolated from an overproducing E. coli strain...
  8. ncbi request reprint Novel roles for classical factors at the interface between translation termination and initiation
    R Karimi
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala, Sweden
    Mol Cell 3:601-9. 1999
    ..We show that this step requires initiation factor IF3, whose role was previously thought to be restricted to promoting specific 30S initiation complex formation from free 30S subunits...
  9. pmc Dissociation rates of peptidyl-tRNA from the P-site of E.coli ribosomes
    R Karimi
    Department of Molecular Biology, BMC, Box 590, S 751 24, Sweden
    EMBO J 15:1149-54. 1996
    ..The results are also used to interpret some in vivo experiments on translational processivity...
  10. ncbi request reprint Shutdown in protein synthesis due to the expression of mini-genes in bacteria
    V Dincbas
    Department of Cell and Molecular Biology, BMC, Uppsala, S 75124, Sweden
    J Mol Biol 291:745-59. 1999
    ..It is proposed that the retranslation by ribosomes of mini-gene transcripts with efficient ribosome binding (Shine/Dalgarno) sequences strongly contributes to the inhibitory effects of mini-gene expression on protein synthesis...
  11. pmc Fast recycling of Escherichia coli ribosomes requires both ribosome recycling factor (RRF) and release factor RF3
    M Y Pavlov
    Department of Molecular Biology, BMC, Uppsala, Sweden
    EMBO J 16:4134-41. 1997
    ..It is proposed that terminating ribosomes become mobile on mRNA and ready to enter the next translation round only after two distinct steps, catalysed consecutively by RF3 and RRF, which are slow in the absence of these factors...
  12. ncbi request reprint Mutations in 23 S ribosomal RNA perturb transfer RNA selection and can lead to streptomycin dependence
    N Bilgin
    Department of Molecular Biology, University of Uppsala, Sweden
    J Mol Biol 235:813-24. 1994
    ..Streptomycin restores viability by increasing the association rate of ternary complex to these doubly altered ribosomes. The binding rate of EF-G to 2661C ribosomes is also reduced while the translocation rate is unaffected...
  13. pmc A post-translational modification in the GGQ motif of RF2 from Escherichia coli stimulates termination of translation
    V Dincbas-Renqvist
    Department of Cell and Molecular Biology and Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, S 751 24, Uppsala, Sweden
    EMBO J 19:6900-7. 2000
    ..We propose that the previously observed lethal effect of overproducing E.coli K12 RF2 arises through accumulating the defects due to lack of Gln252 methylation and Thr246 in place of alanine...
  14. ncbi request reprint A posttermination ribosomal complex is the guanine nucleotide exchange factor for peptide release factor RF3
    A V Zavialov
    Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Cell 107:115-24. 2001
    ..Dissociation of RF3 from the ribosome requires GTP hydrolysis. Our data suggest that RF3 and its eukaryotic counterpart, eRF3, have mechanistic principles in common...
  15. pmc Origins of minigene-dependent growth inhibition in bacterial cells
    V Heurgué-Hamard
    Department of Cell and Molecular Biology, BMC, Box 596, S 75124 Uppsala, Sweden
    EMBO J 19:2701-9. 2000
    ..The peptidyl-tRNA hydrolase rap mutant, unlike the wild-type enzyme, is highly sensitive to the length and sequence of the peptide. Together, these parameters explain the length dependence of mini-gene toxicity...
  16. ncbi request reprint Molecular clocks reduce plasmid loss rates: the R1 case
    J Paulsson
    Department of Cell and Molecular Biology, Biomedical Center Box 596, Uppsala, SE 75124, Sweden
    J Mol Biol 297:179-92. 2000
    ..This simplicity makes it possible to extend the analysis to other replicons with similar design principles...
  17. pmc Ribosomal RNA and protein mutants resistant to spectinomycin
    N Bilgin
    Department of Molecular Biology, Uppsala University, Sweden
    EMBO J 9:735-9. 1990
    ..All of these ribosomal mutants confer a degree of Spc resistance for the EF-G cycle in vitro that is correlated with the degree of growth rate resistance to the antibiotic in culture...
  18. pmc The accuracy of codon recognition by polypeptide release factors
    D V Freistroffer
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Box 596, S 75124 Uppsala, Sweden
    Proc Natl Acad Sci U S A 97:2046-51. 2000
    ..Two codons, UAU and UGG, stand out as hotspots for RF-dependent premature termination...
  19. ncbi request reprint Requirements for rapid plasmid ColE1 copy number adjustments: a mathematical model of inhibition modes and RNA turnover rates
    J Paulsson
    Department of Molecular Biology, Uppsala University, Uppsala, S 75124, Sweden
    Plasmid 39:215-34. 1998
    ..It is also discussed how the presence of the Rom protein may affect dynamic qualities of copy number control...
  20. ncbi request reprint Rate, accuracy and cost of ribosomes in bacterial cells
    M Lovmar
    Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, S 751 24 Uppsala, Sweden
    Biochimie 88:951-61. 2006
    ..e. error levels about 1 per million. The future prospect of extensive integration of biochemistry, cell physiology and population genetics is discussed in the light of the maximal fitness theory and other, similar, theoretical approaches...
  21. ncbi request reprint Comparison of repressor and transcriptional attenuator systems for control of amino acid biosynthetic operons
    J Elf
    Department of Cell and Molecular Biology, BMC, SE-751 24 Uppsala, Sweden
    J Mol Biol 313:941-54. 2001
    ..The reason for this is that repressor, but not attenuator, mechanisms can regulate expression from biosynthetic operons also when transfer RNAs are fully charged with amino acids so that the ribosomes work with maximal speed...
  22. ncbi request reprint Random signal fluctuations can reduce random fluctuations in regulated components of chemical regulatory networks
    J Paulsson
    Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, Uppsala, 75124 Sweden
    Phys Rev Lett 84:5447-50. 2000
    ..The principle resembles Stochastic Resonance in that signal noise improves signal detection...
  23. ncbi request reprint Solution structure of the ternary complex between aminoacyl-tRNA, elongation factor Tu, and guanosine triphosphate
    N Bilgin
    Department of Molecular Biology, The Biomedical Center, Uppsala University, Sweden
    Biochemistry 37:8163-72. 1998
    ..Furthermore, for the hybrid complex in solution there is no evidence for the formation of trimers as suggested by the crystal structure...
  24. ncbi request reprint The mRNA cap structure stimulates rate of poly(A) removal and amplifies processivity of degradation
    J Martinez
    Department of Cell and Molecular Biology, Uppsala University, Box 596, Uppsala SE 751 24, Sweden
    J Biol Chem 276:27923-9. 2001
    ..Complex formation does not require a cap structure on the RNA substrate. The multiple effects of cap are all accounted for by a simple, kinetic model that takes the processivity of PARN into account...
  25. pmc Release factor RF3 in E.coli accelerates the dissociation of release factors RF1 and RF2 from the ribosome in a GTP-dependent manner
    D V Freistroffer
    Department of Molecular Biology, Uppsala University, BMC, Sweden
    EMBO J 16:4126-33. 1997
    ..A model is proposed which explains how RF3 recycles RF1 and RF2 by displacing the factors from the ribosome after the release of peptide...
  26. pmc Fluctuations and quality of control in biological cells: zero-order ultrasensitivity reinvestigated
    O G Berg
    Department of Molecular Evolution, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden
    Biophys J 79:1228-36. 2000
    ..As a consequence, the average response in a large population of cells will often be much more gradual than predicted from macroscopic descriptions...
  27. pmc Stochastic focusing: fluctuation-enhanced sensitivity of intracellular regulation
    J Paulsson
    Department of Cell and Molecular Biology, Biomedical Center Box 596, SE 75124 Uppsala, Sweden
    Proc Natl Acad Sci U S A 97:7148-53. 2000
    ....
  28. ncbi request reprint Kinetic properties of Escherichia coli ribosomes with altered forms of S12
    N Bilgin
    University of Uppsala, Department of Molecular Biology, Sweden
    J Mol Biol 224:1011-27. 1992
    ..We use our in vitro findings to discuss the in vivo physiology of these mutants as well as mechanistic features of E. coli translation...

Research Grants4

  1. Biochemical and Structural Analysis of the Ribosome
    Mans Ehrenberg; Fiscal Year: 2007
    ..Between 30,000 and 100,000 images will be collected for 10-12A resolution. The density maps will be analyzed by manual or automated fitting of x-ray structures or by real space refinement to obtain atomic models. ..