Joshua Tobias

Summary

Country: Sweden

Publications

  1. doi request reprint Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli
    Joshua Tobias
    Department of Microbiology and Immunology, University of Gothenburg, P O Box 435, S 40530 Gothenburg, Sweden
    Microbiol Res 167:564-70. 2012
  2. doi request reprint Strategies to overexpress enterotoxigenic Escherichia coli (ETEC) colonization factors for the construction of oral whole-cell inactivated ETEC vaccine candidates
    Joshua Tobias
    Gothenburg University Vaccine Research Institute GUVAX and Department of Microbiology and Immunology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
    Appl Microbiol Biotechnol 93:2291-300. 2012
  3. doi request reprint Construction of a non-toxigenic Escherichia coli oral vaccine strain expressing large amounts of CS6 and inducing strong intestinal and serum anti-CS6 antibody responses in mice
    Joshua Tobias
    University of Gothenburg Vaccine Research Institute, and WHO Collaborating Center for Research on Enterotoxigenic Escherichia coli, Department of Microbiology and Immunology, The Sahlgrenska Academy of University of Gothenburg, S 40530 Gothenburg, Sweden
    Vaccine 29:8863-9. 2011
  4. doi request reprint Construction of non-toxic Escherichia coli and Vibrio cholerae strains expressing high and immunogenic levels of enterotoxigenic E. coli colonization factor I fimbriae
    Joshua Tobias
    Institute of Biomedicine, Department of Microbiology and Immunology, The Sahlgrenska Academy of Göteborg University, S 40530 Göteborg, Sweden
    Vaccine 26:743-52. 2008
  5. doi request reprint Over-expression of major colonization factors of enterotoxigenic Escherichia coli, alone or together, on non-toxigenic E. coli bacteria
    Joshua Tobias
    University of Gothenburg Vaccine Research Institute, Department of Microbiology and Immunology, The Sahlgrenska Academy of University of Gothenburg, Gothenburg, Sweden
    Vaccine 28:6977-84. 2010
  6. doi request reprint Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6
    Joshua Tobias
    WHO Collaborating Centre for Research on Enterotoxigenic Escherichia coli ETEC, and Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg University, 40530 Goteborg, Sweden
    Vaccine 26:5373-80. 2008
  7. doi request reprint Construction and expression of immunogenic hybrid enterotoxigenic Escherichia coli CFA/I and CS2 colonization fimbriae for use in vaccines
    Joshua Tobias
    University of Gothenburg Vaccine Research Institute GUVAX, 40530 Gothenburg, Sweden
    Appl Microbiol Biotechnol 87:1355-65. 2010
  8. pmc Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coli
    Lena Jansson
    Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Goteborg, Sweden
    PLoS ONE 4:e4487. 2009
  9. ncbi request reprint Mutations in the periplasmic chaperone leading to loss of surface expression of the colonization factor CS6 in enterotoxigenic Escherichia coli (ETEC) clinical isolates
    Matilda Nicklasson
    Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg University, P O Box 435, 405 30 Goteborg, Sweden
    Microb Pathog 44:246-54. 2008
  10. ncbi request reprint Preexisting antibodies to homologous colonization factors and heat-labile toxin in serum, and the risk to develop enterotoxigenic Escherichia coli-associated diarrhea
    Joshua Tobias
    Institute of Biomedicine, Department of Microbiology and Immunology, The Sahlgrenska Academy of Göteborg University, S 40530 Göteborg, Sweden
    Diagn Microbiol Infect Dis 60:229-31. 2008

Collaborators

Detail Information

Publications12

  1. doi request reprint Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli
    Joshua Tobias
    Department of Microbiology and Immunology, University of Gothenburg, P O Box 435, S 40530 Gothenburg, Sweden
    Microbiol Res 167:564-70. 2012
    ..By applying the developed multiplex PCR system, categorization of E. coli isolates isolated from stool samples of infants with diarrhea into the main diarrheagenic E. coli categories is also shown...
  2. doi request reprint Strategies to overexpress enterotoxigenic Escherichia coli (ETEC) colonization factors for the construction of oral whole-cell inactivated ETEC vaccine candidates
    Joshua Tobias
    Gothenburg University Vaccine Research Institute GUVAX and Department of Microbiology and Immunology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
    Appl Microbiol Biotechnol 93:2291-300. 2012
    ..We therefore conclude that our constructs may be useful as candidate strains in an oral whole-cell inactivated CF ETEC vaccine...
  3. doi request reprint Construction of a non-toxigenic Escherichia coli oral vaccine strain expressing large amounts of CS6 and inducing strong intestinal and serum anti-CS6 antibody responses in mice
    Joshua Tobias
    University of Gothenburg Vaccine Research Institute, and WHO Collaborating Center for Research on Enterotoxigenic Escherichia coli, Department of Microbiology and Immunology, The Sahlgrenska Academy of University of Gothenburg, S 40530 Gothenburg, Sweden
    Vaccine 29:8863-9. 2011
    ..Our data indicate that the described phenol-inactivated non-toxigenic and CS6 over-expressing E. coli strain may be a useful component in an oral ETEC vaccine...
  4. doi request reprint Construction of non-toxic Escherichia coli and Vibrio cholerae strains expressing high and immunogenic levels of enterotoxigenic E. coli colonization factor I fimbriae
    Joshua Tobias
    Institute of Biomedicine, Department of Microbiology and Immunology, The Sahlgrenska Academy of Göteborg University, S 40530 Göteborg, Sweden
    Vaccine 26:743-52. 2008
    ..cholerae bacteria also induce strong CFA/I-specific serum IgA and IgG+M responses. We conclude that our constructs may be useful as candidate strains in an oral killed CF-ETEC vaccine...
  5. doi request reprint Over-expression of major colonization factors of enterotoxigenic Escherichia coli, alone or together, on non-toxigenic E. coli bacteria
    Joshua Tobias
    University of Gothenburg Vaccine Research Institute, Department of Microbiology and Immunology, The Sahlgrenska Academy of University of Gothenburg, Gothenburg, Sweden
    Vaccine 28:6977-84. 2010
    ..We conclude that the described type of recombinant bacteria over-expressing major CFs of ETEC, alone or in combination, may be useful as candidate strains for use in an oral whole-cell CF-ETEC vaccine...
  6. doi request reprint Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6
    Joshua Tobias
    WHO Collaborating Centre for Research on Enterotoxigenic Escherichia coli ETEC, and Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg University, 40530 Goteborg, Sweden
    Vaccine 26:5373-80. 2008
    ..These findings may have important implications for the construction of strains expressing high levels of CS6 on the surface for eventual use in an ETEC vaccine...
  7. doi request reprint Construction and expression of immunogenic hybrid enterotoxigenic Escherichia coli CFA/I and CS2 colonization fimbriae for use in vaccines
    Joshua Tobias
    University of Gothenburg Vaccine Research Institute GUVAX, 40530 Gothenburg, Sweden
    Appl Microbiol Biotechnol 87:1355-65. 2010
    ..Bacteria expressing hybrid fimbriae are potential candidate strains in an oral-killed CF-ETEC vaccine, and the approach represents an attractive and novel means of producing a broad-spectrum ETEC vaccine...
  8. pmc Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coli
    Lena Jansson
    Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Goteborg, Sweden
    PLoS ONE 4:e4487. 2009
    ..g. humans and rabbits, but lacking in species not affected by CS6 ETEC, e.g. mice. The ability of CS6-expressing ETEC to adhere to sulfatide in target small intestinal epithelium may thus contribute to virulence...
  9. ncbi request reprint Mutations in the periplasmic chaperone leading to loss of surface expression of the colonization factor CS6 in enterotoxigenic Escherichia coli (ETEC) clinical isolates
    Matilda Nicklasson
    Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg University, P O Box 435, 405 30 Goteborg, Sweden
    Microb Pathog 44:246-54. 2008
    ..The study emphasizes the importance of using both genotypic and phenotypic methods in epidemiological studies of ETEC, e.g. for vaccine development...
  10. ncbi request reprint Preexisting antibodies to homologous colonization factors and heat-labile toxin in serum, and the risk to develop enterotoxigenic Escherichia coli-associated diarrhea
    Joshua Tobias
    Institute of Biomedicine, Department of Microbiology and Immunology, The Sahlgrenska Academy of Göteborg University, S 40530 Göteborg, Sweden
    Diagn Microbiol Infect Dis 60:229-31. 2008
    ..The data suggest an inverse association between the antibody titers against colonization factors, but not heat-labile toxin, and development of ETEC-associated diarrhea...
  11. doi request reprint Vaccines against enterotoxigenic Escherichia coli
    Ann Mari Svennerholm
    Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Box 435, S 40530, Gothenburg, Sweden
    Expert Rev Vaccines 7:795-804. 2008
    ..Against this background, intense efforts are in progress to try to improve the immunogenicity of different available candidate vaccines, as well as to develop new types of ETEC vaccines...
  12. pmc The major subunit, CfaB, of colonization factor antigen i from enterotoxigenic Escherichia coli is a glycosphingolipid binding protein
    Lena Jansson
    Department of Medical Biochemistry, Institute of Biomedicine, Goteborg University, P O Box 440, S 405 30 Goteborg, Sweden
    Infect Immun 74:3488-97. 2006
    ..coli expressing CFA/I in the absence of tip protein CfaE, as well as by purified fimbriae from the same strain. This demonstrates that the glycosphingolipid-binding capacity of CFA/I resides in the major CfaB subunit...