Research Topics
| Joshua TobiasSummaryCountry: Sweden Publications
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Publications
Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coliJoshua Tobias
Department of Microbiology and Immunology, University of Gothenburg, P O Box 435, S 40530 Gothenburg, Sweden
Microbiol Res 167:564-70. 2012..By applying the developed multiplex PCR system, categorization of E. coli isolates isolated from stool samples of infants with diarrhea into the main diarrheagenic E. coli categories is also shown...- Strategies to overexpress enterotoxigenic Escherichia coli (ETEC) colonization factors for the construction of oral whole-cell inactivated ETEC vaccine candidatesJoshua Tobias
Gothenburg University Vaccine Research Institute GUVAX and Department of Microbiology and Immunology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Appl Microbiol Biotechnol 93:2291-300. 2012..We therefore conclude that our constructs may be useful as candidate strains in an oral whole-cell inactivated CF ETEC vaccine... - Construction of a non-toxigenic Escherichia coli oral vaccine strain expressing large amounts of CS6 and inducing strong intestinal and serum anti-CS6 antibody responses in miceJoshua Tobias
University of Gothenburg Vaccine Research Institute, and WHO Collaborating Center for Research on Enterotoxigenic Escherichia coli, Department of Microbiology and Immunology, The Sahlgrenska Academy of University of Gothenburg, S 40530 Gothenburg, Sweden
Vaccine 29:8863-9. 2011..Our data indicate that the described phenol-inactivated non-toxigenic and CS6 over-expressing E. coli strain may be a useful component in an oral ETEC vaccine... - Construction of non-toxic Escherichia coli and Vibrio cholerae strains expressing high and immunogenic levels of enterotoxigenic E. coli colonization factor I fimbriaeJoshua Tobias
Institute of Biomedicine, Department of Microbiology and Immunology, The Sahlgrenska Academy of Göteborg University, S 40530 Göteborg, Sweden
Vaccine 26:743-52. 2008..cholerae bacteria also induce strong CFA/I-specific serum IgA and IgG+M responses. We conclude that our constructs may be useful as candidate strains in an oral killed CF-ETEC vaccine... - Over-expression of major colonization factors of enterotoxigenic Escherichia coli, alone or together, on non-toxigenic E. coli bacteriaJoshua Tobias
University of Gothenburg Vaccine Research Institute, Department of Microbiology and Immunology, The Sahlgrenska Academy of University of Gothenburg, Gothenburg, Sweden
Vaccine 28:6977-84. 2010..We conclude that the described type of recombinant bacteria over-expressing major CFs of ETEC, alone or in combination, may be useful as candidate strains for use in an oral whole-cell CF-ETEC vaccine... - Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6Joshua Tobias
WHO Collaborating Centre for Research on Enterotoxigenic Escherichia coli ETEC, and Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg University, 40530 Goteborg, Sweden
Vaccine 26:5373-80. 2008..These findings may have important implications for the construction of strains expressing high levels of CS6 on the surface for eventual use in an ETEC vaccine... - Construction and expression of immunogenic hybrid enterotoxigenic Escherichia coli CFA/I and CS2 colonization fimbriae for use in vaccinesJoshua Tobias
University of Gothenburg Vaccine Research Institute GUVAX, 40530 Gothenburg, Sweden
Appl Microbiol Biotechnol 87:1355-65. 2010..Bacteria expressing hybrid fimbriae are potential candidate strains in an oral-killed CF-ETEC vaccine, and the approach represents an attractive and novel means of producing a broad-spectrum ETEC vaccine... 
Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coliLena Jansson
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Goteborg, Sweden
PLoS ONE 4:e4487. 2009..g. humans and rabbits, but lacking in species not affected by CS6 ETEC, e.g. mice. The ability of CS6-expressing ETEC to adhere to sulfatide in target small intestinal epithelium may thus contribute to virulence...
Mutations in the periplasmic chaperone leading to loss of surface expression of the colonization factor CS6 in enterotoxigenic Escherichia coli (ETEC) clinical isolatesMatilda Nicklasson
Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg University, P O Box 435, 405 30 Goteborg, Sweden
Microb Pathog 44:246-54. 2008..The study emphasizes the importance of using both genotypic and phenotypic methods in epidemiological studies of ETEC, e.g. for vaccine development...- Preexisting antibodies to homologous colonization factors and heat-labile toxin in serum, and the risk to develop enterotoxigenic Escherichia coli-associated diarrheaJoshua Tobias
Institute of Biomedicine, Department of Microbiology and Immunology, The Sahlgrenska Academy of Göteborg University, S 40530 Göteborg, Sweden
Diagn Microbiol Infect Dis 60:229-31. 2008..The data suggest an inverse association between the antibody titers against colonization factors, but not heat-labile toxin, and development of ETEC-associated diarrhea... - Vaccines against enterotoxigenic Escherichia coliAnn Mari Svennerholm
Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Box 435, S 40530, Gothenburg, Sweden
Expert Rev Vaccines 7:795-804. 2008..Against this background, intense efforts are in progress to try to improve the immunogenicity of different available candidate vaccines, as well as to develop new types of ETEC vaccines... - The major subunit, CfaB, of colonization factor antigen i from enterotoxigenic Escherichia coli is a glycosphingolipid binding proteinLena Jansson
Department of Medical Biochemistry, Institute of Biomedicine, , P.O. Box 440, , Sweden
Infect Immun 74:3488-97. 2006..coli expressing CFA/I in the absence of tip protein CfaE, as well as by purified fimbriae from the same strain. This demonstrates that the glycosphingolipid-binding capacity of CFA/I resides in the major CfaB subunit...