Mikael Kubista

Summary

Affiliation: TATAA Biocenter
Country: Sweden

Publications

  1. ncbi request reprint Nucleic acid-based technologies: application amplified
    Mikael Kubista
    TATAA Biocenter AB, Gothenburg, Sweden
    Pharmacogenomics 5:767-73. 2004
  2. ncbi request reprint The real-time polymerase chain reaction
    Mikael Kubista
    TATAA Biocenter, Medicinargatan 7B, 405 30 Goteborg, Sweden
    Mol Aspects Med 27:95-125. 2006
  3. doi request reprint Gene expression profiling--Clusters of possibilities
    Anders Bergkvist
    MultiD Analyses AB, Gothenburg, Sweden
    Methods 50:323-35. 2010
  4. doi request reprint RT-qPCR work-flow for single-cell data analysis
    Anders Ståhlberg
    TATAA Biocenter, Gothenburg, Sweden Sahlgrenska Cancer Center, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
    Methods 59:80-8. 2013
  5. ncbi request reprint Comparison of reverse transcriptases in gene expression analysis
    Anders Ståhlberg
    Department of Chemistry and Biosciences, Chalmers University of Technology, Gothenburg, Sweden
    Clin Chem 50:1678-80. 2004
  6. pmc Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli
    Anders Ståhlberg
    TATAA Biocenter, Odinsgatan 28, 411 03 Göteborg, Sweden
    BMC Genomics 9:170. 2008
  7. ncbi request reprint Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis
    Kristina Lind
    Chalmers University of Technology, Gothenburg, Sweden
    Biotechniques 40:315-9. 2006
  8. ncbi request reprint Thermodynamics study of the dimerization equilibria of rhodamine B and 6G in different ionic strengths by photometric titration and chemometrics method
    Jahanbakhsh Ghasemi
    Chemistry Department, Faculty of Sciences, Razi University, Kermanshah 57166, Iran
    Spectrochim Acta A Mol Biomol Spectrosc 62:649-56. 2005
  9. pmc Plasticity response in the contralesional hemisphere after subtle neurotrauma: gene expression profiling after partial deafferentation of the hippocampus
    Daniel Andersson
    Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
    PLoS ONE 8:e70699. 2013
  10. ncbi request reprint Quantitative real-time PCR for cancer detection: the lymphoma case
    Anders Ståhlberg
    TATAA Biocenter, Medicinaregatan 7B, 413 90 Gothenburg, Sweden
    Expert Rev Mol Diagn 5:221-30. 2005

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Nucleic acid-based technologies: application amplified
    Mikael Kubista
    TATAA Biocenter AB, Gothenburg, Sweden
    Pharmacogenomics 5:767-73. 2004
  2. ncbi request reprint The real-time polymerase chain reaction
    Mikael Kubista
    TATAA Biocenter, Medicinargatan 7B, 405 30 Goteborg, Sweden
    Mol Aspects Med 27:95-125. 2006
    ..Typical uses of real-time PCR include pathogen detection, gene expression analysis, single nucleotide polymorphism (SNP) analysis, analysis of chromosome aberrations, and most recently also protein detection by real-time immuno PCR...
  3. doi request reprint Gene expression profiling--Clusters of possibilities
    Anders Bergkvist
    MultiD Analyses AB, Gothenburg, Sweden
    Methods 50:323-35. 2010
    ..Reports are either hypothesis generating, from an exploratory study, or hypothesis validating, from a confirmatory study, or both. In either case, we suggest the generated or validated hypotheses be specifically stated...
  4. doi request reprint RT-qPCR work-flow for single-cell data analysis
    Anders Ståhlberg
    TATAA Biocenter, Gothenburg, Sweden Sahlgrenska Cancer Center, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
    Methods 59:80-8. 2013
    ..The aim of this workflow is provide guide to the rapidly growing community studying single-cells by means of reverse transcription quantitative real-time PCR profiling...
  5. ncbi request reprint Comparison of reverse transcriptases in gene expression analysis
    Anders Ståhlberg
    Department of Chemistry and Biosciences, Chalmers University of Technology, Gothenburg, Sweden
    Clin Chem 50:1678-80. 2004
  6. pmc Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli
    Anders Ståhlberg
    TATAA Biocenter, Odinsgatan 28, 411 03 Göteborg, Sweden
    BMC Genomics 9:170. 2008
    ..Here we perform a multiway study of the temporal response of four yeast Saccharomyces cerevisiae strains with different glucose uptake rates upon altered metabolic conditions...
  7. ncbi request reprint Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis
    Kristina Lind
    Chalmers University of Technology, Gothenburg, Sweden
    Biotechniques 40:315-9. 2006
    ..The probe signal reflect formation of target product, while melting curve analysis of the BOXTO signal reveals primer-dimer formation and the presence of any other anomalous products...
  8. ncbi request reprint Thermodynamics study of the dimerization equilibria of rhodamine B and 6G in different ionic strengths by photometric titration and chemometrics method
    Jahanbakhsh Ghasemi
    Chemistry Department, Faculty of Sciences, Razi University, Kermanshah 57166, Iran
    Spectrochim Acta A Mol Biomol Spectrosc 62:649-56. 2005
    ..From the thermodynamic results the TDeltaS degrees -DeltaH degrees plot was sketched. It shows a fairly good positive correlation which indicates the enthalpy-entropy compensation in the dimerization reactions (compensation effect)...
  9. pmc Plasticity response in the contralesional hemisphere after subtle neurotrauma: gene expression profiling after partial deafferentation of the hippocampus
    Daniel Andersson
    Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
    PLoS ONE 8:e70699. 2013
    ..We conclude that even a very limited focal neurotrauma triggers a distinct response at the contralesional side, which at least to some extent depends on astrocyte activation. ..
  10. ncbi request reprint Quantitative real-time PCR for cancer detection: the lymphoma case
    Anders Ståhlberg
    TATAA Biocenter, Medicinaregatan 7B, 413 90 Gothenburg, Sweden
    Expert Rev Mol Diagn 5:221-30. 2005
    ..Fine-needle aspirates and even single cells contain enough material for accurate real-time PCR analysis...
  11. ncbi request reprint Properties of the reverse transcription reaction in mRNA quantification
    Anders Ståhlberg
    Department of Chemistry and Bioscience, Chalmers University of Technology, Gothenburg, Sweden
    Clin Chem 50:509-15. 2004
    ..In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression...
  12. pmc Kinetic Outlier Detection (KOD) in real-time PCR
    Tzachi Bar
    Department of Chemistry and Bioscience, Chalmers University of Technology, Medicinargatan 7B, 405 30 Gothenburg, Sweden
    Nucleic Acids Res 31:e105. 2003
    ..05. This precision is higher than the minimal 2-fold difference in number of DNA molecules that real-time PCR usually aims to detect. Thus, KOD may be a useful tool for outlier detection in real-time PCR...
  13. ncbi request reprint Spectrophotometric and thermodynamic study on the dimerization equilibrium of ionic dyes in water by chemometrics method
    Ali Niazi
    Department of Chemistry, Faculty of Sciences, Azad University of Arak, Arak, Iran
    Spectrochim Acta A Mol Biomol Spectrosc 65:73-8. 2006
    ..The enthalpy and entropy of the dimerization reactions were determined from the dependence of the equilibrium constants to the temperature (van't Hoff equation)...
  14. ncbi request reprint Neural cell adhesion molecule-deficient beta-cell tumorigenesis results in diminished extracellular matrix molecule expression and tumour cell-matrix adhesion
    Joakim Håkansson
    Department of Medical Biochemistry, Goteborg University, Sweden
    Tumour Biol 26:103-12. 2005
    ..Prospective consequences of these findings for the role of N-CAM in beta tumour cell dissemination are discussed...
  15. pmc Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte
    Radek Sindelka
    Laboratory of Gene Expression, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Nucleic Acids Res 36:387-92. 2008
    ..mRNA expression profiles did not change following in vitro fertilization and we conclude that the cortical rotation that follows fertilization has no detectable effect on intracellular mRNA gradients...
  16. pmc A new minor groove binding asymmetric cyanine reporter dye for real-time PCR
    Martin Bengtsson
    Department of Chemistry and Bioscience, Chalmers University of Technology 41296 Göteborg and TATAA Biocenter, Medicinaregatan 9E, 413 90 Goteborg, Sweden
    Nucleic Acids Res 31:e45. 2003
    ..1 and 0.4 micro M generated sufficient fluorescence signal without inhibiting the PCR. A comparison with the commonly used reporter dye SYBR Green I shows that the two dyes behave similarly in all important aspects...
  17. doi request reprint Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction
    Pamela Pinzani
    Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy
    Hum Pathol 39:1474-82. 2008
    ..The approach is expected to become a very powerful tool for expression studies in human cancers at both mRNA and protein levels...
  18. ncbi request reprint Development and evaluation of three real-time immuno-PCR assemblages for quantification of PSA
    Kristina Lind
    Department of Chemistry and Bioscience, Chalmers University of Technology, and TATAA Biocenter, Box 462, 405 30 Gothenburg, Sweden
    J Immunol Methods 304:107-16. 2005
    ..Measurements of PSA in serum samples using real-time immuno-PCR correlated well with measurements performed with ELISA. The real-time immuno-PCR measurements were more sensitive and the dynamic range was larger than with the ELISA...
  19. ncbi request reprint LightUp probes in clinical diagnostics
    Mikael Leijon
    LightUp Technologies AB, Lunastigen 5, SE 141 44 Huddinge, Sweden
    Mol Aspects Med 27:160-75. 2006
    ..An example of cancer diagnostics by detection of altered gene expression patterns will also be shown...
  20. ncbi request reprint Monitoring differentiation of human embryonic stem cells using real-time PCR
    Karin Noaksson
    Cellartis AB, Arvid Wallgrens Backe 20, 413 46 Goteborg, Sweden
    Stem Cells 23:1460-7. 2005
    ..The combination of QPCR with hESC-based technologies opens novel avenues for high-throughput analysis of hESCs in, for example, pharmacological and cytotoxicity screening...
  21. pmc Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels
    Martin Bengtsson
    Department of Experimental Medical Science, Lund University, 221 84 Lund, Sweden
    Genome Res 15:1388-92. 2005
    ..Hence, the geometric mean of expression levels provides a better estimate of gene activity of the typical cell than does the arithmetic mean measured on a cell population...
  22. ncbi request reprint Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression
    Anders Ståhlberg
    Department of Molecular Biotechnology, Chalmers University of Technology, 405 30 Gothenburg, Sweden
    Clin Chem 49:51-9. 2003
    ..As a first step toward a quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed a method for determination of IgLkappa:IgLlambda ratio in clinical samples...