Affiliation: Swedish University of Agricultural Sciences
- Detection of programmed cell death in plant embryosLada H Filonova
Department of Wood Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
Methods Mol Biol 427:173-9. 2008..This chapter provides step-by-step procedure of how to process embryos for TUNEL and how to combine TUNEL with immunolocalization of the protein of interest...
- Metacaspase-dependent programmed cell death is essential for plant embryogenesisMaria F Suarez
Curr Biol 14:R339-40. 2004
- Autophagy and cell-death proteases in plants: two wheels of a funeral cartPeter Bozhkov
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden
Autophagy 3:136-8. 2007..Here we focus on the relationship between autophagic and proteoloytic components in plant PCD at the cellular and organismal levels...
- Aspasing out metacaspases and caspases: proteases of many tradesPeter V Bozhkov
Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7080, SE 75007 Uppsala, Sweden
Sci Signal 3:pe48. 2010..The identification of a common biological target of caspases and metacaspases and previously unknown functions unrelated to cell death of metacaspases provide new food for thought...
- VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formationP V Bozhkov
1Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080, SE 750 07 Uppsala, Sweden
Cell Death Differ 11:175-82. 2004..Our data indicate that the VEIDase activity is an integral part in the control of plant developmental cell death programme, and that this activity is essential for the embryo pattern formation...
- Cysteine protease mcII-Pa executes programmed cell death during plant embryogenesisPeter V Bozhkov
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080, SE 75007 Uppsala, Sweden
Proc Natl Acad Sci U S A 102:14463-8. 2005..Although mcII-Pa and metazoan caspases have different substrate specificity, they serve a common function during development, demonstrating the evolutionary parallelism of PCD pathways in plants and animals...
- Developmental regulation of a VEIDase caspase-like proteolytic activity in barley caryopsisMats Boren
Department of Plant Biology and Forestry Genetics, Swedish University of Agricultural Sciences, PO Box 7080, SE 75007, Uppsala, Sweden
J Exp Bot 57:3747-53. 2006..Several manifestations of programmed cell death exist in developing barley caryopsis, indicating a connection between VEIDase activity and developmental programmed cell death in barley...
- Assessment of the integral membrane protein topology in living cellsAndrey A Zamyatnin
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences SLU, Genetic Centre, Box 7080, SE 750 07 Uppsala, Sweden
Plant J 46:145-54. 2006....
- High stability of nuclear microsatellite loci during the early stages of somatic embryogenesis in Norway spruceAndreas Helmersson
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7080, SE 750 07 Uppsala, Sweden
Tree Physiol 24:1181-6. 2004..We conclude, therefore, that the high genetic stability of somatic embryos in Norway spruce is unaffected by the induction of programmed cell death caused by withdrawal of auxin and cytokinin...
- The level of free intracellular zinc mediates programmed cell death/cell survival decisions in plant embryosAndreas Helmersson
Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences, SE 750 07 Uppsala, Sweden
Plant Physiol 147:1158-67. 2008..Our data demonstrate that perturbation of zinc homeostasis disrupts the balance between cell proliferation and PCD required for plant embryogenesis. This establishes zinc as an important cue governing cell fate decisions in plants...
- A key developmental switch during Norway spruce somatic embryogenesis is induced by withdrawal of growth regulators and is associated with cell death and extracellular acidificationPeter V Bozhkov
Department of Forest Genetics, Uppsala Genetic Centre, Swedish University of Agricultural Sciences, Box 7027, SE 750 07 Uppsala, Sweden
Biotechnol Bioeng 77:658-67. 2002..Over 800 plants regenerated from all tested genotypes demonstrated a good performance in the greenhouse and they were transferred to the field...
- Variation in transcript abundance during somatic embryogenesis in gymnospermsClaudio Stasolla
Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
Tree Physiol 24:1073-85. 2004..Several of these genes encode proteins involved in detoxification processes, methionine synthesis and utilization, and carbohydrate metabolism. The potential role of these genes in embryo development is discussed...
- Re-organisation of the cytoskeleton during developmental programmed cell death in Picea abies embryosAndrei P Smertenko
The Integrative Cell Biology Laboratory, School of Biological Sciences and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK
Plant J 33:813-24. 2003..F-actin de-polymerisation drugs abolish normal embryonic pattern formation and associated PCD in the suspensor, strongly suggesting that the actin network is vital in this PCD pathway...
- Up, down and up again is a signature global gene expression pattern at the beginning of gymnosperm embryogenesisLeonel van Zyl
Forest Biotechnology Group, Centennial Campus, Box 7247, North Carolina State University, Raleigh, NC 27695, USA
Gene Expr Patterns 3:83-91. 2003..abies embryogenesis. The majority of the marker genes encode for proteins involved in translation and posttranslational modification. Among them, 18 genes displayed the major expression pattern...
- Early selection improves clonal performance and reduces intraclonal variation of Norway spruce plants propagated by somatic embryogenesisKarl Anders Högberg
The Forestry Research Institute of Sweden, Ekebo, SE 268 90 Svalöv, Sweden
Tree Physiol 23:211-6. 2003..We conclude that selection according to these criteria at ex vitro transfer can result in improved performance of clonal stock propagated by somatic embryogenesis...