G von Heijne

Summary

Affiliation: Stockholm University
Country: Sweden

Publications

  1. ncbi request reprint Inter-helical hydrogen bond formation during membrane protein integration into the ER membrane
    Marika Hermansson
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91, Stockholm, Sweden
    J Mol Biol 334:803-9. 2003
  2. ncbi request reprint Transmembrane helices before, during, and after insertion
    Stephen H White
    Department of Physiology and Biophysics, and Program in Macromolecular Structure, University of California at Irvine, Irvine, CA 92697 4560, USA
    Curr Opin Struct Biol 15:378-86. 2005
  3. pmc Interface connections of a transmembrane voltage sensor
    J Alfredo Freites
    Department of Physiology and Biophysics and Program in Macromolecular Structure, University of California, Irvine, CA 92697 4560, USA
    Proc Natl Acad Sci U S A 102:15059-64. 2005
  4. ncbi request reprint Membrane topology of the human seipin protein
    Carolina Lundin
    Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm, Sweden
    FEBS Lett 580:2281-4. 2006
  5. pmc Asn- and Asp-mediated interactions between transmembrane helices during translocon-mediated membrane protein assembly
    Nadja M Meindl-Beinker
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 106 91 Stockholm, Sweden
    EMBO Rep 7:1111-6. 2006
  6. ncbi request reprint The membrane protein universe: what's out there and why bother?
    G von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    J Intern Med 261:543-57. 2007
  7. ncbi request reprint Membrane-protein topology
    Gunnar von Heijne
    Center for Biomembrane Research and Stockholm Bioinformatics Center, Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm
    Nat Rev Mol Cell Biol 7:909-18. 2006
  8. ncbi request reprint Microbiology. Translocation of anthrax toxin: lord of the rings
    Gunnar von Heijne
    Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm, Sweden
    Science 309:709-10. 2005
  9. doi request reprint Membrane proteins: from bench to bits
    Gunnar von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Sweden
    Biochem Soc Trans 39:747-50. 2011
  10. doi request reprint Introduction to theme "membrane protein folding and insertion"
    Gunnar von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm Unviersity, S 10691 Stockholm, Sweden
    Annu Rev Biochem 80:157-60. 2011

Collaborators

Detail Information

Publications96

  1. ncbi request reprint Inter-helical hydrogen bond formation during membrane protein integration into the ER membrane
    Marika Hermansson
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91, Stockholm, Sweden
    J Mol Biol 334:803-9. 2003
    ....
  2. ncbi request reprint Transmembrane helices before, during, and after insertion
    Stephen H White
    Department of Physiology and Biophysics, and Program in Macromolecular Structure, University of California at Irvine, Irvine, CA 92697 4560, USA
    Curr Opin Struct Biol 15:378-86. 2005
    ..A detailed analysis of the TM amino acid distributions of helix-bundle membrane proteins of known structure recapitulates this code...
  3. pmc Interface connections of a transmembrane voltage sensor
    J Alfredo Freites
    Department of Physiology and Biophysics and Program in Macromolecular Structure, University of California, Irvine, CA 92697 4560, USA
    Proc Natl Acad Sci U S A 102:15059-64. 2005
    ..It suggests that bilayer phospholipids can adapt locally to strongly perturbing protein elements, causing the phospholipids to become a structural extension of the protein...
  4. ncbi request reprint Membrane topology of the human seipin protein
    Carolina Lundin
    Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm, Sweden
    FEBS Lett 580:2281-4. 2006
    ..Our results suggest that the predominant form of seipin is 462 residues long and has an N(cyt)-C(cyt) orientation with a long luminal loop between the two transmembrane helices...
  5. pmc Asn- and Asp-mediated interactions between transmembrane helices during translocon-mediated membrane protein assembly
    Nadja M Meindl-Beinker
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 106 91 Stockholm, Sweden
    EMBO Rep 7:1111-6. 2006
    ..Our observations suggest that inter-helix hydrogen bonds can form during Sec61 translocon-assisted insertion and thus could be important for membrane protein assembly...
  6. ncbi request reprint The membrane protein universe: what's out there and why bother?
    G von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    J Intern Med 261:543-57. 2007
    ..What is special about membrane proteins in terms of structure and function, how many membrane proteins are out there, how are they made in the cell? Welcome to the membrane protein universe!..
  7. ncbi request reprint Membrane-protein topology
    Gunnar von Heijne
    Center for Biomembrane Research and Stockholm Bioinformatics Center, Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm
    Nat Rev Mol Cell Biol 7:909-18. 2006
    ....
  8. ncbi request reprint Microbiology. Translocation of anthrax toxin: lord of the rings
    Gunnar von Heijne
    Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm, Sweden
    Science 309:709-10. 2005
  9. doi request reprint Membrane proteins: from bench to bits
    Gunnar von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Sweden
    Biochem Soc Trans 39:747-50. 2011
    ..This raises questions regarding how membrane proteins are inserted into the membrane and fold in vivo, and also complicates life for bioinformaticians trying to predict membrane protein topology and structure...
  10. doi request reprint Introduction to theme "membrane protein folding and insertion"
    Gunnar von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm Unviersity, S 10691 Stockholm, Sweden
    Annu Rev Biochem 80:157-60. 2011
    ..Assembly of Bacterial Inner Membrane Proteins." In this short introduction, I discuss these reviews in the larger context of where the field of membrane protein biochemistry is heading...
  11. pmc Formation of transmembrane helices in vivo--is hydrophobicity all that matters?
    Gunnar von Heijne
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Gen Physiol 129:353-6. 2007
  12. pmc Improved membrane protein topology prediction by domain assignments
    Andreas Bernsel
    Stockholm University, Sweden
    Protein Sci 14:1723-8. 2005
    ..Two-thirds of these proteins are single spanning, a group of proteins for which current topology prediction methods perform particularly poorly...
  13. pmc Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli
    Karl Skoog
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    J Bacteriol 194:584-6. 2012
    ..Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event...
  14. pmc Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
    J W de Gier
    Department of Biochemistry, Arrhenius Laboratory, Stockholm University, S 106 91 Stockholm University, Sweden
    Proc Natl Acad Sci U S A 95:14646-51. 1998
    ..The observed change in assembly mechanism when the hydrophobicity of the procoat signal peptide is increased demonstrates that the assembly of an inner membrane protein can be rerouted between different pathways...
  15. pmc Different conformations of nascent polypeptides during translocation across the ER membrane
    I Mingarro
    Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    BMC Cell Biol 1:3. 2000
    ....
  16. ncbi request reprint Effects of 'hydrophobic mismatch' on the location of transmembrane helices in the ER membrane
    M Monne
    Department of Biochemistry and Biophysics, Stockholm University, S 106 91, Stockholm, Sweden
    FEBS Lett 496:96-100. 2001
    ..We discuss these findings in the context of models for peptide-lipid interactions during hydrophobic mismatch...
  17. ncbi request reprint Formation of helical hairpins during membrane protein integration into the endoplasmic reticulum membrane. Role of the N and C-terminal flanking regions
    M Hermansson
    Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden
    J Mol Biol 313:1171-9. 2001
    ..These observations have interesting implications for the way we picture membrane protein topogenesis within the context of the endoplasmic reticulum (ER) translocon...
  18. ncbi request reprint The internal repeats in the Na+/Ca2+ exchanger-related Escherichia coli protein YrbG have opposite membrane topologies
    A Saaf
    Department of Biochemistry and Biophysics, Stockholm University, S 106 91 Stockholm, Sweden
    J Biol Chem 276:18905-7. 2001
    ..This has implications for our understanding of the function of Na(+)/Ca(2+) exchangers and provides an example of "divergent" evolution of membrane protein topology...
  19. pmc Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues
    A Saaf
    Department of Biochemistry, Stockholm University, S 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 96:8540-4. 1999
    ..This example of divergent evolution of membrane protein topology suggests that a complete inversion of membrane topology is possible with relatively few mutational changes even for proteins with multiple transmembrane segments...
  20. ncbi request reprint Inhibition of protein translocation across the endoplasmic reticulum membrane by sterols
    I Nilsson
    Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden
    J Biol Chem 276:41748-54. 2001
    ..The inhibiting effect on translocation is different between different sterols. Our data suggest that the protein translocation machinery may be sensitive to changes in cholesterol levels in the ER membrane...
  21. ncbi request reprint Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli
    D E Drew
    Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, S-106 91 Stockholm, Sweden
    FEBS Lett 507:220-4. 2001
    ..coli. Furthermore, we show that an overexpressed MP can be recovered from a MP-GFP fusion using a site specific protease. This makes GFP an excellent tool for large-scale MP target selection in structural genomics projects...
  22. ncbi request reprint Prediction of organellar targeting signals
    O Emanuelsson
    Stockholm Bioinformatics Center, Stockholm University, S-10691, Stockholm, Sweden
    Biochim Biophys Acta 1541:114-9. 2001
    ..This review attempts to summarize the present state of the art in the field...
  23. ncbi request reprint Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli
    A Saaf
    Department of Biochemistry, Stockholm University, S 106 91 Stockholm, Sweden
    J Biol Chem 273:30415-8. 1998
    ..In contrast to partial N-terminal fusion protein constructs, the full-length protein folds into a protease-resistant conformation, suggesting that important folding determinants are present in the C-terminal part of the molecule...
  24. ncbi request reprint Getting greasy: how transmembrane polypeptide segments integrate into the lipid bilayer
    G von Heijne
    Department of Biochemistry, Stockholm University, Sweden
    Mol Microbiol 24:249-53. 1997
    ..The molecular basis for this remarkable intramembraneous sorting event is a major focus of current studies of membrane protein biogenesis...
  25. ncbi request reprint Membrane insertion of a potassium-channel voltage sensor
    Tara Hessa
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Science 307:1427. 2005
    ..Our results suggest that the paddle model is not inconsistent with the high charge content of S4...
  26. ncbi request reprint Recognition of transmembrane helices by the endoplasmic reticulum translocon
    Tara Hessa
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Nature 433:377-81. 2005
    ..Our results indicate that direct protein-lipid interactions are critical during translocon-mediated membrane insertion...
  27. ncbi request reprint Membrane-protein engineering
    I Mingarro
    Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden
    Trends Biotechnol 15:432-7. 1997
    ..Therefore our ability to understand them and manipulate their functions is both important and necessary to enable protein engineers to create 'designer' membrane proteins (that is, proteins designed to have desired properties)...
  28. doi request reprint Membrane-integration characteristics of two ABC transporters, CFTR and P-glycoprotein
    Karl Enquist
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 387:1153-64. 2009
    ..Membrane-insertion characteristics can thus vary widely between related proteins...
  29. pmc Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for N(in)-C(out) and N(out)-C(in) transmembrane helices
    Carolina Lundin
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 105:15702-7. 2008
    ....
  30. ncbi request reprint Emulating membrane protein evolution by rational design
    Mikaela Rapp
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Science 315:1282-4. 2007
    ..This simple evolutionary pathway can explain the frequent occurrence of membrane proteins with an internal pseudo-two-fold symmetry axis in the plane of the membrane...
  31. pmc Prediction of membrane-protein topology from first principles
    Andreas Bernsel
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 105:7177-81. 2008
    ..This result suggests that prediction of membrane-protein topology and structure directly from first principles is an attainable goal, given the recently improved understanding of peptide recognition by the translocon...
  32. ncbi request reprint Membrane protein structure: prediction versus reality
    Arne Elofsson
    Center for Biomembrane Research, Stockholm Bioinformatics Center, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Annu Rev Biochem 76:125-40. 2007
    ..But how well do the current prediction methods perform? Which structural features can be predicted and which cannot? And what can we expect in the next few years?..
  33. ncbi request reprint Global topology analysis of the Escherichia coli inner membrane proteome
    Daniel O Daley
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Science 308:1321-3. 2005
    ....
  34. ncbi request reprint Reliability measures for membrane protein topology prediction algorithms
    Karin Melén
    Department of Biochemistry and Biophysics, Stockholm Bioinformatics Center, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 327:735-44. 2003
    ....
  35. doi request reprint Prediction of the human membrane proteome
    Linn Fagerberg
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
    Proteomics 10:1141-9. 2010
    ..A visualization tool displaying the topologies suggested by the eight prediction methods, for all predicted membrane proteins, is available on the public Human Protein Atlas portal (www.proteinatlas.org)...
  36. doi request reprint Repositioning of transmembrane alpha-helices during membrane protein folding
    Anni Kauko
    Center for Biomembrane Research and Stockholm Bioinformatics Center, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 397:190-201. 2010
    ..These observations raise new challenges for the structure prediction of membrane proteins and suggest that the classical two-stage model often used to describe membrane protein folding needs to be modified...
  37. ncbi request reprint Cleavage-site motifs in mitochondrial targeting peptides
    Y Gavel
    Department of Theoretical Physics, Royal Institute of Technology, Stockholm, Sweden
    Protein Eng 4:33-7. 1990
    ..We also suggest that a well-conserved RXY decreases (S/A) pattern may be used to engineer efficiently recognized cleavage sites into uncleaved or artificial mitochondrial targeting peptides...
  38. ncbi request reprint Structural aspects of transmembrane alpha-helices
    G von Heijne
    Department of Biochemistry, Stockholm University, Sweden
    Acta Physiol Scand Suppl 643:17-9. 1998
    ..In this short communication, a new way to study the conformation and membrane localization of a single transmembrane protein segment--glycosylation mapping--is introduced...
  39. ncbi request reprint N-tail translocation in a eukaryotic polytopic membrane protein: synergy between neighboring transmembrane segments
    M Monne
    Department of Biochemistry, Stockholm University, Stockholm, Sweden
    Eur J Biochem 263:264-9. 1999
    ..Our observations imply a non-sequential assembly mechanism in which the ultimate location of the N-tail relative to the membrane may depend on more than one transmembrane segment...
  40. doi request reprint Control of membrane protein topology by a single C-terminal residue
    Susanna Seppälä
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Science 328:1698-700. 2010
    ..This observation points to an unanticipated plasticity in membrane protein insertion mechanisms...
  41. ncbi request reprint Membrane protein assembly in vivo
    Gunnar von Heijne
    Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm, Sweden
    Adv Protein Chem 63:1-18. 2003
  42. ncbi request reprint How hydrophobic is alanine?
    Ingmarie Nilsson
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Biol Chem 278:29389-93. 2003
    ....
  43. pmc A global topology map of the Saccharomyces cerevisiae membrane proteome
    Hyun Kim
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 103:11142-7. 2006
    ..By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes...
  44. ncbi request reprint Analysis of transmembrane helix integration in the endoplasmic reticulum in S. cerevisiae
    Tara Hessa
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 386:1222-8. 2009
    ..cerevisiae and the mammalian ER, we find that the two scales are strongly correlated but that the absolute difference between the most hydrophobic and most hydrophilic residues is approximately 2-fold smaller in S. cerevisiae...
  45. ncbi request reprint Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
    J W de Gier
    Department of Biochemistry, Arrhenius Laboratory, Stockholm University, Sweden
    FEBS Lett 399:307-9. 1996
    ..coli SRP. Depletion of either component strongly affected the insertion of both Lep and Lep-inv into the cytoplasmic membrane. This indicates that SRP is required for the assembly of cytoplasmic membrane proteins in E. coli...
  46. ncbi request reprint A Day in the Life of Dr K. or How I Learned to Stop Worrying and Love Lysozyme: a tragedy in six acts
    G von Heijne
    Department of Biochemistry, Stockholm University, Stockholm, S 106 91, Sweden
    J Mol Biol 293:367-79. 1999
    ..James Glib-Burdock, drama critic for The Stratford Observer...
  47. ncbi request reprint Predicting subcellular localization of proteins based on their N-terminal amino acid sequence
    O Emanuelsson
    Stockholm Bioinformatics Center, Department of Biochemistry, Stockholm University, Stockholm, S 106 91, Sweden
    J Mol Biol 300:1005-16. 2000
    ..TargetP is available as a web-server at http://www.cbs.dtu.dk/services/TargetP/...
  48. ncbi request reprint Comparative analysis of amino acid distributions in integral membrane proteins from 107 genomes
    Johan Nilsson
    Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden
    Proteins 60:606-16. 2005
    ..The results strongly support the notion that a biased distribution of Lys and Arg residues between cytoplasmic and extra-cytoplasmic segments (the positive-inside rule) is present in most if not all organisms...
  49. pmc LumenP--a neural network predictor for protein localization in the thylakoid lumen
    Isabelle Westerlund
    Stockholm Bioinformatics Center, and Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Protein Sci 12:2360-6. 2003
    ..The combination of TargetP and LumenP predicts around 1.5%-3% of all proteins encoded in the genomes of Arabidopsis thaliana and Oryza sativa to be located in the lumen of the thylakoid...
  50. ncbi request reprint A study of the membrane-water interface region of membrane proteins
    Erik Granseth
    Stockholm Bioinformatics Center, AlbaNova, SE 106 91 Stockholm, Sweden
    J Mol Biol 346:377-85. 2005
    ..The interface region thus imposes different constraints on protein structure than do the central hydrocarbon core of the membrane and the surrounding aqueous phase...
  51. ncbi request reprint Competition between neighboring topogenic signals during membrane protein insertion into the ER
    Magnus Monné
    Department of Biochemistry and Biophysics, Stockholm University, Sweden
    FEBS J 272:28-36. 2005
    ..We suggest that the topology of the TM1-TM2 region of Cig30 depends on a competition between TM1 and TM2 such that the transmembrane segment that inserts first into the ER membrane determines the final topology...
  52. pmc Stable insertion of Alzheimer Abeta peptide into the ER membrane strongly correlates with its length
    Carolina Lundin
    Stockholm Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius vag 12, SE 106 91 Stockholm, Sweden
    FEBS Lett 581:3809-13. 2007
    ..Our results show a strong correlation between the length of an Abeta-derived segment and its ability to integrate into the microsomal membrane...
  53. doi request reprint Membrane insertion of marginally hydrophobic transmembrane helices depends on sequence context
    Linnea E Hedin
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 396:221-9. 2010
    ..However, for at least two of the marginally hydrophobic helices, the local interactions are not enough, indicating that post-insertional rearrangements are involved in the folding of these proteins...
  54. ncbi request reprint Molecular code for transmembrane-helix recognition by the Sec61 translocon
    Tara Hessa
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Nature 450:1026-30. 2007
    ..The emerging picture of translocon-mediated transmembrane helix assembly is simple, with the critical sequence characteristics mirroring the physical properties of the lipid bilayer...
  55. ncbi request reprint Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex
    Hyun Kim
    Department of Biochemistry and Biophysics, Stockholm University, SE 10691 Stockholm, Sweden
    J Biol Chem 280:20261-7. 2005
    ..Our results suggest that STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation...
  56. ncbi request reprint Membrane topology of Kch, a putative K+ channel from Escherichia coli
    M Johansson
    Department of Biochemistry, Stockholm University, S 106 91 Stockholm, Sweden
    J Biol Chem 271:25912-5. 1996
    ..The rather hydrophobic P-segment is found to translocate only slowly across the inner membrane and seems to be near a threshold for stop-transfer function...
  57. ncbi request reprint Identification and evolution of dual-topology membrane proteins
    Mikaela Rapp
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, AlbaNova, Stockholm University, SE 10691 Stockholm, Sweden
    Nat Struct Mol Biol 13:112-6. 2006
    ..Our results provide strong support for the existence of dual-topology proteins and shed new light on the evolution of membrane-protein topology and structure...
  58. pmc Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae
    Marie Osterberg
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 103:11148-53. 2006
    ..We also have quantified the expression levels for >550 membrane proteins, facilitating the choice of well expressing proteins for future functional and structural studies...
  59. pmc New Escherichia coli outer membrane proteins identified through prediction and experimental verification
    Paola Marani
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Protein Sci 15:884-9. 2006
    ....
  60. ncbi request reprint Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions
    Hyun Kim
    Department of Biochemistry and Biophysics, Stockholm University, Sweden
    J Biol Chem 278:10208-13. 2003
    ..We propose that this approach may be used to produce reliable topology models on a proteome-wide scale...
  61. ncbi request reprint Assembly of the cytochrome bo3 complex
    Filippa Stenberg
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Sweden
    J Mol Biol 371:765-73. 2007
    ..We also show that cofactor insertion catalyses assembly. These findings provide novel insights into the biogenesis of this model membrane protein complex...
  62. ncbi request reprint Experimentally constrained topology models for 51,208 bacterial inner membrane proteins
    Erik Granseth
    Stockholm Bioinformatics Center, AlbaNova, SE 106 91 Stockholm, Sweden
    J Mol Biol 352:489-94. 2005
    ..The 51,208 much improved topology models derived in this way cover approximately 30% of all predicted bacterial inner membrane proteins in 225 fully sequenced bacterial genomes...
  63. doi request reprint Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter
    Marika Cassel
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Mol Biol 381:860-6. 2008
    ..Since the 3-D structure of ClcA is known, our results provide a critical test of the reporter fusion approach and offer new insights into the ClcA folding pathway...
  64. pmc Contribution of positively charged flanking residues to the insertion of transmembrane helices into the endoplasmic reticulum
    Mirjam Lerch-Bader
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 105:4127-32. 2008
    ....
  65. ncbi request reprint Cleavage of a tail-anchored protein by signal peptidase
    Ingmarie Nilsson
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91, Stockholm, Sweden
    FEBS Lett 516:106-8. 2002
    ..Here, we show that a tail-anchored protein can be cleaved by signal peptidase and that the sequence requirements for efficient cleavage seem to be the same as for cleavage of co-translationally targeted SRP-dependent proteins...
  66. ncbi request reprint Membrane protein structural biology--how far can the bugs take us?
    Erik Granseth
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    Mol Membr Biol 24:329-32. 2007
    ..With this technical limitation in mind, we have probed the question as to how far prokaryotic homologues can take us towards a structural understanding of the eukaryotic/human membrane proteome(s)...
  67. pmc Improved detection of homologous membrane proteins by inclusion of information from topology predictions
    Maria Hedman
    Stockholm Bioinformatics Center, SCFAB, Stockholm University, SE 10691, Stockholm, Sweden
    Protein Sci 11:652-8. 2002
    ..Benchmarking over a test set of G-protein-coupled receptor sequences shows that the number of false positives is significantly reduced in this way, both when closely related and distantly related proteins are searched for...
  68. pmc Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis
    David Drew
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 99:2690-5. 2002
    ..We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the approximately 800 inner-membrane proteins thought to exist in E. coli...
  69. ncbi request reprint In silico prediction of the peroxisomal proteome in fungi, plants and animals
    Olof Emanuelsson
    Stockholm Bioinformatics Center, AlbaNova University Center, Department of Biochemistry and Biophysics, Stockholm University, S 106 91, Stockholm, Sweden
    J Mol Biol 330:443-56. 2003
    ..These proteins can be grouped into 29 families covering most of the known steps in all known peroxisomal pathways. In general, plants have the highest number of predicted peroxisomal proteins, and fungi the smallest number...
  70. pmc Stop-transfer efficiency of marginally hydrophobic segments depends on the length of the carboxy-terminal tail
    Tara Hessa
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    EMBO Rep 4:178-83. 2003
    ..Our findings suggest that membrane integration of a hydrophobic segment is not simply a question of thermodynamic equilibrium, but can be influenced by details of the translocation mechanism...
  71. ncbi request reprint Locating proteins in the cell using TargetP, SignalP and related tools
    Olof Emanuelsson
    Stockholm Bioinformatics Center, AlbaNova, Stockholm University, SE 10691 Stockholm, Sweden
    Nat Protoc 2:953-71. 2007
    ..In addition, we describe and provide web references to other useful subcellular localization predictors. Finally, we discuss predictive performance measures in general and the performance of TargetP and SignalP in particular...
  72. doi request reprint How translocons select transmembrane helices
    Stephen H White
    Department of Physiology and Biophysics, University of California, Irvine, California 92697 4560, USA
    Annu Rev Biophys 37:23-42. 2008
    ..We review this progress and consider the connection between the physical principles of membrane protein stability and translocon selection of transmembrane helices...
  73. pmc Prediction of lipoprotein signal peptides in Gram-negative bacteria
    Agnieszka S Juncker
    Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby 2800, Denmark
    Protein Sci 12:1652-62. 2003
    ..A neural network-based predictor was developed for comparison, and it gave very similar results. LipoP is available as a Web server at www.cbs.dtu.dk/services/LipoP/...
  74. pmc Membrane assembly of the cannabinoid receptor 1: impact of a long N-terminal tail
    Helena Andersson
    Yale University, Department of Molecular Biophysics and Biochemistry, P O Box 208114, New Haven, CT 06520 8114, USA
    Mol Pharmacol 64:570-7. 2003
    ..We propose that the N-tail translocation is a crucial early step in biosynthesis of the receptor and may play a role in regulating the stability and surface expression of CB1...
  75. pmc Determination of the membrane topology of Ost4p and its subunit interactions in the oligosaccharyltransferase complex in Saccharomyces cerevisiae
    Hyun Kim
    Department of Biochemistry and Cell Biology and the Institute for Cell and Developmental Biology, Stony Brook University, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 100:7460-4. 2003
    ....
  76. pmc Photocross-linking of nascent chains to the STT3 subunit of the oligosaccharyltransferase complex
    Ingmarie Nilsson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    J Cell Biol 161:715-25. 2003
    ....
  77. pmc Prediction of partial membrane protein topologies using a consensus approach
    Johan Nilsson
    Stockholm Bioinformatics Centre, AlbaNova, Sweden
    Protein Sci 11:2974-80. 2002
    ..Reliably predicted partial topologies may simplify experimental determinations of membrane protein topology...
  78. ncbi request reprint Membrane proteins: shaping up
    Chen Ni Chin
    Department Molecular Biophysics and Biochemistry, Yale University, Bass 429, 266 Whitney Avenue, New Haven, CT 06520, USA
    Trends Biochem Sci 27:231-4. 2002
    ....
  79. ncbi request reprint Insertion and topology of a plant viral movement protein in the endoplasmic reticulum membrane
    Marçal Vilar
    Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, E 46 100 Burjassot, Spain
    J Biol Chem 277:23447-52. 2002
    ....
  80. ncbi request reprint Determination of N- and C-terminal borders of the transmembrane domain of integrin subunits
    Anne Stefansson
    Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, SE 751 23 Uppsala, Sweden
    J Biol Chem 279:21200-5. 2004
    ..Instead the shift results in a shorter and therefore less tilted or bent TM alpha-helix...
  81. ncbi request reprint Protein complexes of the Escherichia coli cell envelope
    Filippa Stenberg
    Department of Biochemistry and Stockholm University, Stockholm SE 106 91, Sweden
    J Biol Chem 280:34409-19. 2005
    ..coli membrane protein complexes during different growth conditions and in different mutant backgrounds...
  82. ncbi request reprint A nine-transmembrane domain topology for presenilin 1
    Hanna Laudon
    Department of Neurotec, Division of Experimental Geriatrics, Karolinska Institutet, Novum, SE 141 86 Huddinge, Sweden
    J Biol Chem 280:35352-60. 2005
    ....
  83. ncbi request reprint Helices on the move
    Gunnar von Heijne
    Nat Struct Mol Biol 12:834-5. 2005
  84. pmc Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K+ channel voltage sensor domain
    Liyan Zhang
    Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aobayama 6 6 07, Sendai 980 8579, Japan
    Proc Natl Acad Sci U S A 104:8263-8. 2007
    ..These results suggest that cooperative insertion of the voltage-sensor transmembrane helices is a property common to K(v) channels and that the degree of cooperativity depends on a balance between electrostatic and hydrophobic forces...
  85. ncbi request reprint Membrane proteins up for grabs
    Gunnar von Heijne
    Nat Biotechnol 25:646-7. 2007
  86. ncbi request reprint Membrane topology mapping of vitamin K epoxide reductase by in vitro translation/cotranslocation
    Jian Ke Tie
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:16410-6. 2005
    ..Altogether, our results suggest that VKOR is a type III membrane protein with three transmembrane domains, which agrees well with the prediction by the topology prediction program TMHMM...
  87. ncbi request reprint The machinery of membrane protein assembly
    Stephen H White
    Department of Physiology and Biophysics, Program in Macromolecular Structure, University of California at Irvine, Irvine, California 92697 4560, USA
    Curr Opin Struct Biol 14:397-404. 2004
    ..The folding of membrane proteins may begin in the ribosome exit tunnel, before entering the translocon, according to cryo-electron microscopy and biophysical studies...
  88. ncbi request reprint Mapping the interaction of the STT3 subunit of the oligosaccharyl transferase complex with nascent polypeptide chains
    Andrey L Karamyshev
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, USA
    J Biol Chem 280:40489-93. 2005
    ..Our results show that the addition of a glycan moiety in a stretch of approximately 15 residues surrounding a QK(*)T cross-linking site impairs the interaction between the nascent chain and STT3...
  89. pmc Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction
    Jean Benoit Peltier
    Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA
    Plant Cell 14:211-36. 2002
    ..Many of the predicted lumenal proteins must be present at concentrations at least 10,000-fold lower than proteins of the photosynthetic apparatus...
  90. pmc Experimentally based topology models for E. coli inner membrane proteins
    Mikaela Rapp
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Protein Sci 13:937-45. 2004
    ..We further report experimentally based topology models for 31 Escherichia coli inner membrane proteins, using methodology suitable for genome-scale studies...
  91. pmc Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125
    Claudine Medigue
    Genoscope, CNRS UMR 8030, Atelier de Génomique Comparative, 91006 Evry Cedex, France
    Genome Res 15:1325-35. 2005
    ..Adding to its originality, P. haloplanktis further differs from its marine counterparts with recruitment of a plasmid origin of replication for its second chromosome...
  92. pmc Topology of the membrane-associated hepatitis C virus protein NS4B
    Marika Lundin
    Karolinska Institutet, Department of Medicine at Center of Molecular Medicine, Karolinska Hospital, S 171 76 Stockholm, Sweden
    J Virol 77:5428-38. 2003
    ..A shared topology achieved in two different ways supports the notion of a common function for NS4B in FLAVIVIRIDAE:..
  93. doi request reprint Membranes: reading between the lines
    Gunnar von Heijne
    Curr Opin Struct Biol 18:403-5. 2008
  94. ncbi request reprint Human neuropeptide Y signal peptide gain-of-function polymorphism is associated with increased body mass index: possible mode of function
    Bo Ding
    Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm SE 171 77, Sweden
    Regul Pept 127:45-53. 2005
    ..Our findings in humans strongly indicate that the NPY signaling system is implicated in body weight regulation and suggest a new and unexpected functional role of a signal peptide...
  95. ncbi request reprint Features of transmembrane segments that promote the lateral release from the translocase into the lipid phase
    Kun Xie
    Department of Chemistry, The Ohio State University, 100 W 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 46:15153-61. 2007
    ..coli system. These results are significant because they define the features of a membrane protein transmembrane segment that induce lateral release from the YidC and Sec translocases into the lipid bilayer in bacteria...
  96. pmc PONGO: a web server for multiple predictions of all-alpha transmembrane proteins
    Mauro Amico
    BioDec Srl, via Calzavecchio, 20 2, I 40033 Casalecchio di Reno, BO, Italy
    Nucleic Acids Res 34:W169-72. 2006
    ..Together with the predicted topology, the server also displays a signal peptide prediction determined with SPEP. The PONGO web server is available at http://pongo.biocomp.unibo.it/pongo...

Research Grants1

  1. New approaches for facilitating membrane protein overexpression and purification
    Gunnar von Heijne; Fiscal Year: 2007
    ..The project aims to develop better techniques for producing membrane proteins on a large scale. ..