Genomes and Genes
Affiliation: University of Gothenburg
Location: Gothenburg, Sweden
Studies of molecular mechanisms and biomarker in autoimmunity.
Autoimmune diseases collectively affect 5-7% of the population, often with debilitating effects. In order to better understand the genes and mechanisms involved in autoimmunity we are studying idiopathic thrombocytopenic purpura (ITP) and multiple sclerosis (MS). In ITP, the platelets are destroyed prematurely, and in MS, axons, oligodendrocytes and myelin. By combining gene expression studies, protein analyses and animal studies we are trying to define the genes and mechanisms that are involved in leukocytes in the target organs of different autoimmune diseases. In the only animal model for ITP we are studying regulated genes in the spleen since this organ is central in ITP and 70% of all ITP patients are cured by splenectomy. Our aim is also to identify differences in mechanisms between the acute and chronic form of ITP and to understand why the acute form spontaneously resolves. Biomarkers are important diagnostic tools and can be used as markers for certain disease mechanisms. We are focused on finding a biomarker in blood since this would simplify the clinical monitoring of ITP and MS patients. Using genome-wide expression profiling of lymfocytes and plasma we also aim to identify unique microRNA profile for ITP and MS. The identification of regulated genes, microRNAs and involved mechanisms may provide new insights into the physiology and pathophysiology of ITP and MS and general mechanisms in autoimmunity.
Studies of molecular mechanisms and biomarker in ITP" 2011 Aroseniusfondens 400 000 kronor
- Regulation of carboxylesterase 1 (CES1) in human adipose tissueMargareta Jernas
Department of Molecular and Clinical Medicine and Center for Cardiovascular and Metabolic Research, The Sahlgrenska Academy, Goteborg, Sweden
Biochem Biophys Res Commun 383:63-7. 2009..1x10(-6)). Basal and stimulated lipolysis was not different in individuals with high, intermediate, and low expression of CES1. Thus, CES1 expression was linked to body fat and adipocyte fat content but not to lipolytic activity...
- Evaluation of CXCL9 and CXCL10 as circulating biomarkers of human cardiac allograft rejectionKristjan Karason
Department of Cardiology, The Sahlgrenska Academy, Göteborgs University, SE 413 45, Goteborg, Sweden
BMC Cardiovasc Disord 6:29. 2006..However, this is an invasive procedure, which is unpleasant for the patient and carries a certain risk. Therefore, a sensitive non-invasive biomarker of acute rejection would be desirable...
- Increased number of B-cells in the red pulp of the spleen in ITPBob Olsson
Department of Psychiatry and Neurochemistry, University of Gothenburg, Molndal, Sweden
Ann Hematol 91:271-7. 2012..In conclusion, B-cells are increased in the red pulp of the spleen and together with cytotoxic T-cells, helper T-cells and macrophages line the sinusoids enabling the immunological attack on platelets in ITP...
- Tenomodulin is highly expressed in adipose tissue, increased in obesity, and down-regulated during diet-induced weight lossAtsuhito Saiki
Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, The Sahlgrenska Academy at Gothenburg University, 413 45 Gothenburg, Sweden
J Clin Endocrinol Metab 94:3987-94. 2009..Global gene expression scans show that the TNMD gene also is expressed in human adipose tissue and that its expression is regulated in response to weight reduction; however, more detailed information is lacking...
- Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucoseKatrina Kos
Diabetes and Endocrinology Research Unit, Clinical Sciences Centre, University Hospital Aintree, Liverpool, UK
Diabetes 58:1780-8. 2009..Adipose tissue collagen has recently been found to be linked with metabolic dysregulation. Therefore, we tested the hypothesis that SPARC in human adipose tissue is influenced by glucose metabolism and adipokines...
- Increased expression of aquaporin 3 in atopic eczemaM Olsson
Department of Pediatrics, Queen Silvia Children s Hospital, and Department of Dermatology, Sahlgrenska Academy, Gothenburg, Sweden
Allergy 61:1132-7. 2006..Dry skin in atopic eczema depends on increased water loss. The mechanisms behind this are poorly understood. The aim of this work was to identify genes that may contribute to water loss in eczema...
- DPP-IV inhibition enhances the antilipolytic action of NPY in human adipose tissueK Kos
Unit for Diabetes and Metabolism, Clinical Sciences Research Institute CSRI, Warwick Medical School, Coventry, UK
Diabetes Obes Metab 11:285-92. 2009..Little is known whether DPP-IV inhibitors for the treatment of type 2 diabetic (T2DM) patients could influence these pathways...
- Changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins in patients with critical illnessMargareta Jernas
Department of Molecular and Clinical Medicine, Sahlgrenska Academy, University of Gothenburg, SE 413 45 Gothenburg, Sweden
Metabolism 58:102-8. 2009..05). The described changes in adipose tissue gene expression and plasma levels of adipokines and acute-phase proteins may influence the development of insulin resistance during critical illness...
- Expression of chemokine (C-C motif) ligand 18 in human macrophages and atherosclerotic plaquesDaniel A Hägg
Center of Metabolic and Cardiovascular Research, Department of Molecular and Clinical Medicine, Sahlgrenska Academy, University of Goteborg, Vita stråket 15, 41345 Goteborg, Sweden
Atherosclerosis 204:e15-20. 2009..Such genes may be important in atherogenesis and pathophysiology of the plaque, and genes that encode for secreted proteins may be potential biomarkers for atherosclerosis and cardiovascular disease...
- Cell death-inducing DFF45-like effector C is reduced by caloric restriction and regulates adipocyte lipid metabolismBjörn Magnusson
Department of Molecular and Clinical Medicine, Institute of Medicine, The Sahlgrenska Academy, Goteborg University, SE 413 45 Goteborg, Sweden
Metabolism 57:1307-13. 2008..Thus, we suggest that CIDEC is a regulator of adipocyte lipid metabolism and may be important for the adipocyte to adapt to changes in energy availability...
- Preliminary report: Zn-alpha2-glycoprotein genotype and serum levels are associated with serum lipidsLouise E Olofsson
The Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, Sahlgrenska Academy at University of Gothenburg, 413 45 Gothenburg, Sweden
Metabolism 59:1316-8. 2010..014) and low-density lipoprotein cholesterol (P = .026) in healthy subjects, and the associations were replicated in an additional cohort (P = .0017 and P = .060, respectively). Our data indicate that ZAG plays a role in lipid metabolism...
- In humans the adiponectin receptor R2 is expressed predominantly in adipose tissue and linked to the adipose tissue expression of MMIF-1K Kos
Diabetes and Endocrinology Clinical Research Group, Clinical Sciences Centre, University Hospital Aintree, Longmoor Lane, Liverpool, L9 7AL, UK
Diabetes Obes Metab 12:360-3. 2010..The adiponectin-dependent insulin-sensitizing action of thiazolidinediones is thus probably to differ amongst species with weaker effects on the human liver...
- Differences in associations between HSD11B1 gene expression and metabolic parameters in subjects with and without impaired glucose homeostasisC Karlsson
Department of Molecular and Clinical Medicine, the Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
Diabetes Res Clin Pract 88:252-8. 2010..We investigated the relationship between HSD11B1 mRNA levels in adipose tissue and in skeletal muscle and anthropometric and metabolic measurements in humans with and without impaired glucose homeostasis...
- Association of sirtuin 1 (SIRT1) gene SNPs and transcript expression levels with severe obesityStephen J Clark
Department of Genomics of Common Diseases, School of Public Health, Imperial College London, Hammersmith Hospital, London, UK
Obesity (Silver Spring) 20:178-85. 2012..However, no SNP was associated with SIRT1 transcript expression level. In summary, both SNPs and SIRT1 gene expression are associated with severe obesity...
- Differential coexpression analysis of obesity-associated networks in human subcutaneous adipose tissueA J Walley
Department of Genomics of Common Disease, School of Public Health, Imperial College London, Hammersmith Hospital, London, UK
Int J Obes (Lond) 36:137-47. 2012....
- Peripheral administration of bovine GH regulates the expression of cerebrocortical beta-globin, GABAB receptor 1, and the Lissencephaly-1 protein (LIS-1) in adult hypophysectomized ratsMarion Walser
Laboratory of Experimental Endocrinology, Department of Internal Medicine, Institute of Medicine, Sahlgrenska University Hospital, University of Gothenburg, Gothenburg, Sweden
Growth Horm IGF Res 21:16-24. 2011..These transcripts represent potential novel mechanisms by which GH can interact with the brain...
- Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNAMaria Eldh
Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
PLoS ONE 5:e15353. 2010..Exosomes differ compared to their donor cells, not only in size, but also in their RNA, protein and lipid composition...
- Gene expression in human brown adipose tissuePer Arne Svensson
Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Int J Mol Med 27:227-32. 2011....
- Identification of adipocyte genes regulated by caloric intakeNiclas Franck
Department of Medical and Health Sciences, Linkoping University, 581 85 Linkoping, Sweden
J Clin Endocrinol Metab 96:E413-8. 2011..Changes in energy intake have marked and rapid effects on metabolic functions, and some of these effects may be due to changes in adipocyte gene expression that precede alterations in body weight...
- Twist1 in human white adipose tissue and obesityAmanda T Pettersson
Karolinska Institutet, Department of Medicine Huddinge, Lipid Laboratory, Stockholm, Sweden
J Clin Endocrinol Metab 96:133-41. 2011..Twist1 is a transcription factor implicated in the regulation of TNFα signaling and was recently shown to be highly expressed in both human and murine adipose tissue, but its role in obesity is unknown...
- Familial dyslexia in a large Swedish family: a whole genome linkage scanIdor Svensson
Department of Psychology, Linnæus University, 351 95 Växjö, Sweden
Behav Genet 41:43-9. 2011..The results were discussed in relation to diagnostic procedures and sample characteristics...
- The autocrine motility factor receptor is overexpressed on the surface of B cells in Binet C chronic lymphocytic leukemiaSofia Grund
Department of Internal Medicine, Section of Hematology and Coagulation, Sahlgrenska University Hospital, University of Gothenburg, 413 45, Gothenburg, Sweden
Med Oncol 28:1542-8. 2011..Cell surface AMFR was increased in Binet C compared with Binet A+B (P=0.016). In conclusion, the mRNA levels reflect the total amount of AMFR, whereas cell surface expression is associated with progression in CLL...
- Regulation of human aldoketoreductase 1C3 (AKR1C3) gene expression in the adipose tissuePer Arne Svensson
Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy at the University of Gothenburg, SE 413 45, Gothenburg, Sweden
Cell Mol Biol Lett 13:599-613. 2008..5-fold, p < 0.01). In conclusion, adipose tissue AKR1C3 expression may be affected by metabolic disease, and its levels are significantly reduced in response to dietinduced weight loss and correlate with leptin levels...
- Expression profiling of macrophages from subjects with atherosclerosis to identify novel susceptibility genesDaniel A Hägg
Department of Molecular and Clinical Medicine, Sahlgrenska Academy, University of Goteborg, Goteborg, Sweden
Int J Mol Med 21:697-704. 2008..Our method provides a reliable approach for identifying susceptibility genes for atherosclerosis, and we conclude that elevated IRS2 gene expression in macrophages may be associated with an increased risk of CHD...
- Identification of genes predominantly expressed in human macrophagesPer Arne Svensson
Department of Internal Medicine, Research Centre for Endocrinology and Metabolism, Vita straket 12, Sahlgrenska Academy, Goteborg University, S 41345 Goteborg, Sweden
Atherosclerosis 177:287-90. 2004..Identification of novel macrophage specific genes may increase our understanding of the role of this cell in different diseases...
- Gene profiling reveals increased expression of uteroglobin and other anti-inflammatory genes in glucocorticoid-treated nasal polypsMikael Benson
Laboratory for Clinical and Experimental Allergy Research, Department of Oto Rhino Laryngology, Malmo University Hospital, Malmo, Sweden
J Allergy Clin Immunol 113:1137-43. 2004..Treatment with local glucocorticoids (GCs) decreases symptoms and the size of nasal polyps. This might depend on the downregulation of proinflammatory genes, as well as the upregulation of anti-inflammatory genes...
- T-cell-mediated cytotoxicity toward platelets in chronic idiopathic thrombocytopenic purpuraBob Olsson
Research Center for Endocrinology and Metabolism, Sahlgrenska University Hospital, Goteborg University, SE 413 45 Goteborg, Sweden
Nat Med 9:1123-4. 2003..In addition, cytotoxic cell-mediated lysis of autologous platelets was shown in active ITP. Our data suggest that T-cell-mediated cytotoxicity is an alternative mechanism for platelet destruction in ITP...
- Copper induces the expression of cholesterogenic genes in human macrophagesPer Arne Svensson
Department of Internal Medicine, Research Centre for Endocrinology and Metabolism RCEM, Vita straket 12, Sahlgrenska Academy, Goteborg University, S 41345 Gothenburg, Sweden
Atherosclerosis 169:71-6. 2003..The effect of copper on cholesterogenic genes may also have implications for liver steatosis in early stages of Wilson's disease...
- High expression of complement components in omental adipose tissue in obese menBritt G Gabrielsson
Research Centre for Endocrinology and Metabolism, Sahlgrenska University Hospital, Goteborg University, Goteborg, Sweden
Obes Res 11:699-708. 2003..Accumulation of visceral fat is recognized as a predictor of obesity-related metabolic disturbances. Factors that are predominantly expressed in this depot could mediate the link between visceral obesity and associated diseases...
- Depot-specific expression of fibroblast growth factors in human adipose tissueBritt G Gabrielsson
Research Centre for Endocrinology and Metabolism and Division for Body Composition and Metabolism, The Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
Obes Res 10:608-16. 2002..We have investigated the expression of several fibroblast growth factors (FGFs) and FGF-receptors (FGFRs) in human adipose tissue and adipose-tissue cell fractions obtained from both subcutaneous (sc) and omental (om) depots...
- DNA microarrays to study gene expression in allergic airwaysM Benson
Allergy Laboratory, Department of Oto Rhino Laryngology, Malmo University Hospital, S 205 02 Malmo, Sweden
Clin Exp Allergy 32:301-8. 2002..Allergic rhinitis results from interactions between a large number of cells and mediators in different compartments of the body. DNA microarrays allow simultaneous measurement of expression of thousands of genes in the same tissue sample...
- Total RNA and array-based expression monitoringB Carlsson
Nat Biotechnol 18:579. 2000
- [Pseudothrombocytopenia--a laboratory disease]J Westin
Lakartidningen 84:555-7. 1987
- Evaluation of reference genes for studies of gene expression in human adipose tissueBritt G Gabrielsson
Research Center for Endocrinology and Metabolism, Department of Internal Medicine, Grona Straket 8, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
Obes Res 13:649-52. 2005..The aim of this study was to evaluate reference genes for expression studies of human adipose tissue...
- Gene expression profiling of the rat hippocampus one month after focal cerebral ischemia followed by enriched environmentAnnica Rönnbäck
Department of Public Health and Clinical Medicine, Umea University Hospital, S 901 85 Umea, Sweden
Neurosci Lett 385:173-8. 2005..Further studies at earlier time points and in additional subregions of the brain are of interest in the search for molecular mechanisms behind EE-induced neuronal plasticity after ischemic stroke...
- Relations of adipose tissue CIDEA gene expression to basal metabolic rate, energy restriction, and obesity: population-based and dietary intervention studiesAnders Gummesson
Department of Molecular and Clinical Medicine and Wallenberg Laboratory for Cardiovascular Research, Institute of Medicine, The Sahlgrenska Academy, Goteborg University, SE 413 45 Goteborg, Sweden
J Clin Endocrinol Metab 92:4759-65. 2007....
- Dissociation between adipose tissue expression and serum levels of adiponectin during and after diet-induced weight loss in obese subjects with and without the metabolic syndromeCarl Johan Behre
Wallenberg Laboratory for Cardiovascular Research, Institute of Medicine, The Sahlgrenska Academy, Goteborg University, SE 413 45 Goteborg, Sweden
Metabolism 56:1022-8. 2007..Weight loss increased serum levels of adiponectin without a parallel increase in adiponectin gene expression. The mechanisms involved in the regulation of adiponectin levels merits further investigation...
- The expression of NAD(P)H:quinone oxidoreductase 1 is high in human adipose tissue, reduced by weight loss, and correlates with adiposity, insulin sensitivity, and markers of liver dysfunctionJenny Palming
Sahlgrenska Center for Cardiovascular and Metabolic Research, Department of Molecular and Clinical Medicine, The Sahlgrenska Academy, Goteborg University, Goteborg, Sweden
J Clin Endocrinol Metab 92:2346-52. 2007..Studies in mice deficient in NQO1 activity suggest that NQO1 may also play an important role in metabolism...
- A network-based analysis of allergen-challenged CD4+ T cells from patients with allergic rhinitisM Benson
Department of Pediatrics, Queen Silvia Children s Hospital, Gothenburg, Sweden
Genes Immun 7:514-21. 2006..05). A logical inference from all this is that the proliferation of allergen-challenged Th2 cells is associated with a decreased apoptosis of Th2 cells and an increase in TNFRSF4 signalling...
- Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expressionMargareta Jernas
Research Centre for Endocrinology and Metabolism, Division of Body Composition and Metabolism, Department of Internal Medicine, Vita stråket 15, SE 413 45 Goteborg, Sweden
FASEB J 20:1540-2. 2006..Thus, we have identified genes with markedly higher expression in large, compared with small, human adipocytes. These genes may link hypertrophic obesity to insulin resistance/type 2 diabetes...
- Increased levels of acylation-stimulating protein in interleukin-6-deficient (IL-6(-/-)) miceI Wernstedt
Research Center for Endocrinology and Metabolism, Wallenberg Laboratory, Sahlgrenska Academy, Sahlgrenska University Hospital, SE 41345 Goteborg, Sweden
Endocrinology 147:2690-5. 2006..This increase may result in increased triacylglycerol formation and uptake in IL-6(-/-) adipocytes and thereby contribute to the development of obesity in IL-6(-/-) mice...
- Differential global gene expression response patterns of human endothelium exposed to shear stress and intraluminal pressureMaria Andersson
Clinical Experimental Research Laboratory, Cardiovascular Institute Sahlgrenska University Hospital Ostra, Goteborg, Sweden
J Vasc Res 42:441-52. 2005..Considering the low number of overlapping genes, we suggest that the endothelial cells can distinguish between shear stress and pressure stimulation...
- Oxidized LDL induces a coordinated up-regulation of the glutathione and thioredoxin systems in human macrophagesDaniel Hagg
Research Centre for Endocrinology and Metabolism, Division of Body Composition and Metabolism, Department of Internal Medicine, Vita stråket 15, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
Atherosclerosis 185:282-9. 2006....
- Major role of HSP70 as a paracrine inducer of cytokine production in human oxidized LDL treated macrophagesPer Arne Svensson
Research Centre for Endocrinology and Metabolism, Division of Body Composition and Metabolism, Department of Internal Medicine, Vita straket 12, Sahlgrenska Academy, Goteborg University, S 413 45 Goteborg, Sweden
Atherosclerosis 185:32-8. 2006..This suggests that extracellular HSP70 can mediate pro-inflammatory changes in macrophages in response to oxLDL...
- MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS)Margareta Jernas
Department of Molecular and Clinical Medicine, the Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
BMC Immunol 14:32. 2013..To investigate the role of microRNA in multiple sclerosis (MS), we performed genome-wide expression analyses of mRNA and microRNA in T-cells from MS patients and controls...
- Differential expression of T-cell genes in blood and bone marrow between ITP patients and controlsMargareta Jernas
Department of Internal Medicine, Institute of Medicine, Sahlgrenska Academy at University of Gothenburg, Vita straket 12, SE 413 45 Gothenburg, Sweden
Thromb Haemost 109:112-7. 2013..This further supports the concept that bone marrow is an important compartment in ITP...