Mathias Uhlen

Summary

Affiliation: Royal Institute of Technology
Country: Sweden

Publications

  1. pmc Assembly of a gene sequence tag microarray by reversible biotin-streptavidin capture for transcript analysis of Arabidopsis thaliana
    Valtteri Wirta
    Department of Molecular Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, SE 106 91, Stockholm, Sweden
    BMC Biotechnol 5:5. 2005
  2. ncbi request reprint A human protein atlas for normal and cancer tissues based on antibody proteomics
    Mathias Uhlen
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 4:1920-32. 2005
  3. ncbi request reprint Antibody-based proteomics for human tissue profiling
    Mathias Uhlen
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 4:384-93. 2005
  4. pmc Antibody-based protein profiling of the human chromosome 21
    Mathias Uhlen
    Science for Life Laboratory, Royal Institute of Technology, SE 17121 Solna, Sweden
    Mol Cell Proteomics 11:M111.013458. 2012
  5. pmc Parallel immunizations of rabbits using the same antigen yield antibodies with similar, but not identical, epitopes
    Barbara Hjelm
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    PLoS ONE 7:e45817. 2012
  6. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011
  7. ncbi request reprint Microbead display of proteins by cell-free expression of anchored DNA
    Olof Nord
    Department of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    J Biotechnol 106:1-13. 2003
  8. ncbi request reprint A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein
    My Hedhammar
    Royal Institute of Technology, AlbaNova University Center, Department of Biotechnology, SE 106 91 Stockholm, Sweden
    J Biotechnol 119:133-46. 2005
  9. ncbi request reprint Multiplexed PrEST immunization for high-throughput affinity proteomics
    Karin Larsson
    Department of Proteomics, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
    J Immunol Methods 315:110-20. 2006
  10. doi request reprint A genecentric Human Protein Atlas for expression profiles based on antibodies
    Lisa Berglund
    Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 7:2019-27. 2008

Detail Information

Publications87

  1. pmc Assembly of a gene sequence tag microarray by reversible biotin-streptavidin capture for transcript analysis of Arabidopsis thaliana
    Valtteri Wirta
    Department of Molecular Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, SE 106 91, Stockholm, Sweden
    BMC Biotechnol 5:5. 2005
    ....
  2. ncbi request reprint A human protein atlas for normal and cancer tissues based on antibody proteomics
    Mathias Uhlen
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 4:1920-32. 2005
    ..Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research...
  3. ncbi request reprint Antibody-based proteomics for human tissue profiling
    Mathias Uhlen
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 4:384-93. 2005
    ....
  4. pmc Antibody-based protein profiling of the human chromosome 21
    Mathias Uhlen
    Science for Life Laboratory, Royal Institute of Technology, SE 17121 Solna, Sweden
    Mol Cell Proteomics 11:M111.013458. 2012
    ..The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed...
  5. pmc Parallel immunizations of rabbits using the same antigen yield antibodies with similar, but not identical, epitopes
    Barbara Hjelm
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    PLoS ONE 7:e45817. 2012
    ..The results suggest that polyclonal antibodies generated by repeated immunizations do not display an identical epitope pattern, although many of the epitopes are similar...
  6. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011
    ....
  7. ncbi request reprint Microbead display of proteins by cell-free expression of anchored DNA
    Olof Nord
    Department of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    J Biotechnol 106:1-13. 2003
    ..The use of this technology, including alternative formats, for different proteomics applications is discussed...
  8. ncbi request reprint A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein
    My Hedhammar
    Royal Institute of Technology, AlbaNova University Center, Department of Biotechnology, SE 106 91 Stockholm, Sweden
    J Biotechnol 119:133-46. 2005
    ..Finally, the newly developed method was used to evaluate two different purification tags, His(6) and Z(basic), and their effect on the expression pattern...
  9. ncbi request reprint Multiplexed PrEST immunization for high-throughput affinity proteomics
    Karin Larsson
    Department of Proteomics, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
    J Immunol Methods 315:110-20. 2006
    ....
  10. doi request reprint A genecentric Human Protein Atlas for expression profiles based on antibodies
    Lisa Berglund
    Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 7:2019-27. 2008
    ..The new version of the protein atlas thus is a resource for many areas of biomedical research, including protein science and biomarker discovery...
  11. ncbi request reprint Gene expression analysis by signature pyrosequencing
    Charlotta Agaton
    Department of Biotechnology, KTH, Royal Institute of Technology SCFAB, Roslagstullsbacken 21, 106 91 Stockholm, Sweden
    Gene 289:31-9. 2002
    ..The analysis resulted in identification of genes with possible relevance for development of atherosclerosis. The use of the method for automated massive parallel signature sequencing is discussed...
  12. ncbi request reprint Microfluidic analysis of antibody specificity in a compact disk format
    Cecilia Eriksson
    Royal Institute of Technology, AlbaNova University Center, Department of Biotechnology, SE 106 91 Stockholm, Sweden
    J Proteome Res 5:1568-74. 2006
    ..The method presented here is shown to be very sensitive, flexible and reproducible...
  13. ncbi request reprint A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products
    Susanne Gräslund
    Division of Molecular Biotechnology, Department of Biotechnology, Royal Institute of Technology KTH, SCFAB, SE 10691 Stockholm, Sweden
    J Biotechnol 99:41-50. 2002
    ..5 M guanidine hydrochloride. The convenience and robustness of the presented expression system should make it highly suitable for various high-throughput protein expression efforts...
  14. pmc The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)
    Joakim Westberg
    Department of Biotechnology, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    Genome Res 14:221-7. 2004
    ....
  15. ncbi request reprint Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies
    Nina Kronqvist
    Department of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Recent Pat Biotechnol 4:171-82. 2010
    ..In addition, strategies for characterization of recombinant proteins on the staphylococcal cell surface, with an emphasis on an approach for epitope mapping of antibodies, are included...
  16. doi request reprint Validation of serum protein profiles by a dual antibody array approach
    Rebecca Rimini
    Department of Proteomics, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteomics 73:252-66. 2009
    ....
  17. doi request reprint A whole-genome bioinformatics approach to selection of antigens for systematic antibody generation
    Lisa Berglund
    School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    Proteomics 8:2832-9. 2008
    ..A genome-wide analysis demonstrates that antigens for approximately 80% of the human protein-coding genes can be selected with this strategy...
  18. ncbi request reprint Using Grid technology for computationally intensive applied bioinformatics analyses
    Jorge Andrade
    Department of Biotechnology, Royal Institute of Technology KTH, Stockholm, Sweden
    In Silico Biol 6:495-504. 2006
    ..This model should be of general interest in applied bioinformatics. Scripts and procedures are freely available from the authors...
  19. ncbi request reprint Single-vector three-frame expression systems for affinity-tagged proteins
    Susanne Gräslund
    Department of Biotechnology, Royal Institute of Technology KTH, 106 91, Stockholm, Sweden
    FEMS Microbiol Lett 215:139-47. 2002
    ..Nevertheless, we believe that the presented results represent the first successful attempt to create single-vector three-frame expression systems, a concept that could become valuable in future combined cloning-expression vectors...
  20. ncbi request reprint Anti-idiotypic protein domains selected from protein A-based affibody libraries
    Malin Eklund
    Department of Biotechnology, Royal Institute of Technology KTH SCFAB, Stockholm, Sweden
    Proteins 48:454-62. 2002
    ..The use of this type of small, robust, and easily expressed anti-idiotypic affibody pair for affinity technology applications, including self-assembled protein networks, is discussed...
  21. ncbi request reprint Construction and characterization of affibody-Fc chimeras produced in Escherichia coli
    Jenny Rönnmark
    Department of Biotechnology, Royal Institute of Technology SCFAB, SE 106 91 Stockholm, Sweden
    J Immunol Methods 261:199-211. 2002
    ..Such "artificial antibodies" should be interesting alternatives to, for example, whole antibodies or scFv-Fc fusions as detection devices and in diagnostic or therapeutic applications...
  22. ncbi request reprint Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues
    Charlotta Agaton
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Roslagsvägen 30 B, 106 91 Stockholm, Sweden
    Mol Cell Proteomics 2:405-14. 2003
    ..The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues...
  23. doi request reprint Large-scale protein profiling in human cell lines using antibody-based proteomics
    Linn Fagerberg
    Department of Proteomics, School of Biotechnology, AlbaNova University Center, KTH Royal Institute of Technology, SE 10691 Stockholm, Sweden
    J Proteome Res 10:4066-75. 2011
    ..This work also provides an extensive list of antibodies, for which high-resolution images as well as validation data are available on the Human Protein Atlas ( www.proteinatlas.org ), that are of potential use in cell line studies...
  24. doi request reprint The correlation between cellular size and protein expression levels--normalization for global protein profiling
    Emma Lundberg
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    J Proteomics 71:448-60. 2008
    ..The results demonstrate the value of large-scale, automated proteome analysis using immunohistochemistry, in revealing functional correlations and establishing methods to interpret and mine proteomic data...
  25. doi request reprint Prediction of the human membrane proteome
    Linn Fagerberg
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
    Proteomics 10:1141-9. 2010
    ..A visualization tool displaying the topologies suggested by the eight prediction methods, for all predicted membrane proteins, is available on the public Human Protein Atlas portal (www.proteinatlas.org)...
  26. doi request reprint Comparative protein profiling of serum and plasma using an antibody suspension bead array approach
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    Proteomics 10:532-40. 2010
    ....
  27. ncbi request reprint Strategy for highly selective ion-exchange capture using a charge-polarizing fusion partner
    Torbjörn Gräslund
    Department of Biotechnology, Royal Institute of Technology KTH, Stockholm, Sweden
    J Chromatogr A 942:157-66. 2002
    ..Methods for site-specific proteolysis of the fusion proteins to release native target proteins are also discussed...
  28. ncbi request reprint Toward a confocal subcellular atlas of the human proteome
    Laurent Barbe
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 7:499-508. 2008
    ..The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization...
  29. ncbi request reprint Selective enrichment of monospecific polyclonal antibodies for antibody-based proteomics efforts
    Charlotta Agaton
    Department of Biotechnology, Royal Institute of Technology, AlbaNova University Center, KTH, Stockholm, Sweden
    J Chromatogr A 1043:33-40. 2004
    ..The results suggest that the protocol can be used in a highly parallel and automated manner to generate mono-specific polyclonal antibodies for large-scale, antibody-based proteomics efforts, i.e. affinity proteomics...
  30. ncbi request reprint Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol
    Jenny Rönnmark
    Department of Biotechnology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    J Immunol Methods 281:149-60. 2003
    ..In addition, using human kidney biopsy samples from a nephropathy patient, IgA depositions in glomeruli could be detected by immunohistochemistry with low background staining of tissue...
  31. pmc Mutational analysis of the interaction between albumin-binding domain from streptococcal protein G and human serum albumin
    Martin Linhult
    Department of Biotechnology, Royal Institute of Technology KTH, SCFAB, S 106 91 Stockholm, Sweden
    Protein Sci 11:206-13. 2002
    ..The analysis shows that the HSA binding involves residues mainly in the second alpha-helix...
  32. doi request reprint Antibodypedia, a portal for sharing antibody and antigen validation data
    Erik Björling
    School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 7:2028-37. 2008
    ..The database thus provides a virtual resource of publicly available antibodies toward human proteins with accompanying experimental evidence supporting an individual validation score for each antibody in an application-specific manner...
  33. doi request reprint Mapping the subcellular protein distribution in three human cell lines
    Linn Fagerberg
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, SE 106 91, Sweden
    J Proteome Res 10:3766-77. 2011
    ..Our results show distinct organelle proteomes, well conserved across the cell types, and demonstrate that biochemically similar organelles are grouped together...
  34. doi request reprint Antibody suspension bead arrays within serum proteomics
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteome Res 7:3168-79. 2008
    ..The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins...
  35. doi request reprint Defining the transcriptome and proteome in three functionally different human cell lines
    Emma Lundberg
    Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden
    Mol Syst Biol 6:450. 2010
    ..Thus, this study shows a path to characterize the transcriptome and proteome in human cells from different origins...
  36. pmc Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays
    Jochen M Schwenk
    Science for Life Laboratory, Royal Institute of Technology KTH, Stockholm, Sweden
    Mol Cell Proteomics 9:2497-507. 2010
    ..The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format...
  37. ncbi request reprint Selection of protein epitopes for antibody production
    Mats Lindskog
    Royal Institute of Technology, Stockholm, Sweden
    Biotechniques 38:723-7. 2005
    ..The software and the strategy were evaluated on a human test data set and verified to fulfill the requested criteria...
  38. pmc Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model
    Frida Danielsson
    Science for Life Laboratory, Royal Institute of Technology KTH, SE 17121 Solna, Sweden
    Proc Natl Acad Sci U S A 110:6853-8. 2013
    ..This shows that this comprehensive demonstration of the molecular changes underlying malignant transformation is a relevant model to study the process of tumor formation...
  39. pmc Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes
    Elton P Hudson
    School of Biotechnology, Alba Nova University Center, Royal Institute of Technology, Stockholm, Sweden
    Sci Rep 2:706. 2012
    ....
  40. doi request reprint Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment
    Maja Neiman
    Royal Institute of Technology, Solna, Sweden
    J Proteome Res 10:4925-34. 2011
    ....
  41. pmc Correlations between RNA and protein expression profiles in 23 human cell lines
    Marcus Gry
    Department of Proteomics, School of Biotechnology, AlbaNova University Center, KTH Royal Institute of Technology, Stockholm, Sweden
    BMC Genomics 10:365. 2009
    ....
  42. ncbi request reprint A web-based tool for in silico biomarker discovery based on tissue-specific protein profiles in normal and cancer tissues
    Erik Björling
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 7:825-44. 2008
    ..The lists of candidate proteins can be used as a starting point for further validation in larger patient cohorts using both immunological approaches and technologies utilizing more classical proteomics tools...
  43. pmc Prediction of antibody response using recombinant human protein fragments as antigen
    Johan Rockberg
    School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, Stockholm SE 106 91, Sweden
    Protein Sci 18:2346-55. 2009
    ..The data set presented here can be used for further studies to design new prediction tools for the generation of antibodies to specific protein targets...
  44. doi request reprint Epitope mapping using gram-positive surface display
    Johan Rockberg
    School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    Curr Protoc Immunol . 2010
    ..The protocol described here has been shown useful for mapping of both monoclonal and polyclonal binders with varying epitope lengths...
  45. ncbi request reprint Determination of binding specificities in highly multiplexed bead-based assays for antibody proteomics
    Jochen M Schwenk
    Department of Proteomics, Royal Institute of Technology KTH, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 6:125-32. 2007
    ..This high throughput analysis will potentially have great impact on the possibility for the utilization of different antibody proteomics approaches where the quality assessment of antibodies is of the utmost importance...
  46. ncbi request reprint Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology
    Torbjörn Gräslund
    Department of Biotechnology, Royal Institute of Technology KTH, SCFAB, S 106 91, Stockholm, Sweden
    J Biotechnol 96:93-102. 2002
    ..The strategy also includes an integrated site-specific removal of the Z(basic) purification handle to yield a free target protein...
  47. ncbi request reprint High-throughput solubility assay for purified recombinant protein immunogens
    Maria Stenvall
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, 106 91 Stockholm, Sweden
    Biochim Biophys Acta 1752:6-10. 2005
    ..This comparison showed only weak correlation between the theoretical and experimental values, which emphasizes the importance of experimental assays to determine the solubility of recombinant proteins...
  48. doi request reprint Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies
    Johan Rockberg
    School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Mol Oncol 3:238-47. 2009
    ..The work describes a new strategy to develop antibodies directed to non-overlapping epitopes and shows a path of pursuit to explore the epitope space of a target protein...
  49. doi request reprint Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
    Charlotte Stadler
    Science for Life Laboratory, Royal Institute of Technology, Stockholm, SE 171 65, Sweden
    J Proteomics 75:2236-51. 2012
    ..With a large fraction of the human proteome still unexplored, we suggest this approach to be of great importance under the continued work of mapping the human proteome on a subcellular level...
  50. doi request reprint A homogeneous assay for protein analysis in droplets by fluorescence polarization
    Haakan N Joensson
    Division of Nanobiotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    Electrophoresis 33:436-9. 2012
    ..This adds to the repertoire of droplet assay techniques a direct protein detection method which can be performed entirely inside droplets without the need for labeling of the analyte molecules...
  51. ncbi request reprint A human protein atlas based on antibody proteomics
    Anja Persson
    Royal Institute of Technology, School of Biotechnology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Curr Opin Mol Ther 8:185-90. 2006
    ..The Human Protein Atlas is related to four other expression atlas initiatives, including, in particular, an upcoming protein atlas developed by the Sanger Institute...
  52. doi request reprint Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase
    Barbara Hjelm
    Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    N Biotechnol 27:129-37. 2010
    ..An alanine-scanning experiment showed binding to four specific residues. The implications for the systematic analysis of antibody epitopes on the basis of these results are discussed...
  53. doi request reprint Automated sample preparation method for mass spectrometry analysis on recombinant proteins
    Johanna Steen
    School of Biotechnology, Department of Proteomics, AlbaNova University Center, Royal Institute of Technology, 106 91 Stockholm, Sweden
    J Chromatogr A 1216:4457-64. 2009
    ....
  54. ncbi request reprint Genome-based proteomics
    Charlotta Agaton
    Royal Institute of Technology, AlbaNova University Center, Dept of Biotechnology, Stockholm, Sweden
    Electrophoresis 25:1280-8. 2004
    ..These technologies are in many ways complementary and should be seen as various ways to elucidate different functions of the proteome...
  55. ncbi request reprint Single cell gene mutation analysis using laser-assisted microdissection of tissue sections
    Asa Persson
    Department of Biotechnology, Royal Institute of Technology KTH, SCFAB, S 106 91 Stockholm, Sweden
    Methods Enzymol 356:334-43. 2002
  56. ncbi request reprint Pyrosequencing analysis of thrombosis-associated risk markers
    Kristina Holmberg
    Department of Biotechnology, Royal Institute of Technology, Stockholm Sweden
    Clin Chem 51:1549-52. 2005
  57. ncbi request reprint High-throughput protein purification using an automated set-up for high-yield affinity chromatography
    Johanna Steen
    School of Biotechnology, Division of Molecular Biotechnology, AlbaNova University Center, KTH, Royal Institute of Technology, Roslagsvägen 30 B, 106 91 Stockholm, Sweden
    Protein Expr Purif 46:173-8. 2006
    ..The procedure is highly reproducible and allows for high protein yield and purity...
  58. doi request reprint A single fixation protocol for proteome-wide immunofluorescence localization studies
    Charlotte Stadler
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    J Proteomics 73:1067-78. 2010
    ....
  59. doi request reprint Creation of an antibody-based subcellular protein atlas
    Emma Lundberg
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
    Proteomics 10:3984-96. 2010
    ..The considerations made when creating the subcellular protein atlas and critical parameters of this approach are discussed...
  60. pmc Analysis of transcript and protein overlap in a human osteosarcoma cell line
    Daniel Klevebring
    Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Royal Institute of Technology, SE 171 65 Solna, Sweden
    BMC Genomics 11:684. 2010
    ....
  61. pmc A transcriptional timetable of autumn senescence
    Anders Andersson
    Department of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Genome Biol 5:R24. 2004
    ..One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence...
  62. pmc Viral and microbial genotyping by a combination of multiplex competitive hybridization and specific extension followed by hybridization to generic tag arrays
    Baback Gharizadeh
    Department of Biotechnology, Stockholm Center for Physics, Astronomy and Biotechnology, Royal Institute of Technology KTH, Roslagstullbacken 21, SE 106 91 Stockholm, Sweden
    Nucleic Acids Res 31:e146. 2003
    ..As a model system, different clinically relevant human papillomaviruses were selected for this study. The method generated accurate results and proves to be promising for specific and correct microbial and viral typing...
  63. ncbi request reprint The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures
    Anders Holmberg
    Department of Biotechnology, Royal Institute of Technology KTH, Stockholm, Sweden
    Electrophoresis 26:501-10. 2005
    ....
  64. ncbi request reprint Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A
    Jenny Rönnmark
    Department of Biotechnology, Royal Institute of Technology SCFAB, Stockholm, Sweden
    Eur J Biochem 269:2647-55. 2002
    ..In addition, efficient affinity recovery of IgA from unconditioned human plasma was also demonstrated...
  65. doi request reprint High nuclear RBM3 expression is associated with an improved prognosis in colorectal cancer
    Barbara Hjelm
    Department of Proteomics, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Proteomics Clin Appl 5:624-35. 2011
    ..In this study, we investigated the prognostic impact of human RBM3 expression in colorectal cancer using tissue microarray-based immunohistochemical analysis...
  66. ncbi request reprint Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling
    Peter Nilsson
    Department of Biotechnology, Royal Institute of Technology KTH, Stockholm, Sweden
    Proteomics 5:4327-37. 2005
    ..The results suggest that a comprehensive atlas containing extensive protein expression and subcellular localization data of the human proteome can be generated in an efficient manner with mono-specific antibodies...
  67. ncbi request reprint A novel method for reproducible fluorescent labeling of small amounts of antibodies on solid phase
    Emma Lundberg
    Department of Biotechnology, Royal Institute of Technology, Roslagstullsbacken 21, SE 106 91 Stockholm, Sweden
    J Immunol Methods 322:40-9. 2007
    ..This novel solid-phase antibody labeling method may also be applicable for other conjugation chemistries and labels, and has potential for high-throughput applications...
  68. doi request reprint The 6th HUPO Antibody Initiative (HAI) workshop: sharing data about affinity reagents and other recent developments. September 2009, Toronto, Canada
    Kalle Jonasson
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
    Proteomics 10:2066-8. 2010
    ..workshop for the HUPO Antibody Initiative (HAI) held in September 2009 was co-chaired by Michael Snyder and Mathias Uhlen and discussed several aspects of antibody production, their validation, and attempts to standardise this ..
  69. doi request reprint Targeted protein pullout from human tissue samples using competitive elution
    Ronny Falk
    Royal Institute of Technology, AlbaNova University Center, Department of Biotechnology, Stockholm, Sweden
    Biotechnol J 6:28-37. 2011
    ..Competitive elution has thus been shown to offer an effective approach for wide-scale pullout experiments where proteins and their interaction partners are to be studied...
  70. doi request reprint Droplet size based separation by deterministic lateral displacement-separating droplets by cell--induced shrinking
    Haakan N Joensson
    Div of Nanobiotechnology, Royal Institute of Technology, KTH, AlbaNova University Center, SE 10691 Stockholm, Sweden
    Lab Chip 11:1305-10. 2011
    ..g. clonal selection. The same device also separates 11 µm from 30 µm droplets at a rate of 12,000 droplets per second, more than twofold faster than previously demonstrated passive hydrodynamic separation devices...
  71. doi request reprint Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody molecule as capture ligand
    Helena Wållberg
    Division of Molecular Biotechnology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    Protein Expr Purif 76:127-35. 2011
    ....
  72. pmc The epitope space of the human proteome
    Lisa Berglund
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Protein Sci 17:606-13. 2008
    ..The implications for human antibody-based proteomics efforts are discussed...
  73. doi request reprint Epitope mapping of antibodies using bacterial surface display
    Johan Rockberg
    Department of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Nat Methods 5:1039-45. 2008
    ..Our approach shows a path forward for systematic validation of antibodies for epitope specificity and cross-reactivity on a whole-proteome level...
  74. doi request reprint Generation and validation of affinity reagents on a proteome-wide level
    Mathias Uhlen
    Department of Proteomics, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    J Mol Recognit 22:57-64. 2009
    ..proteinatlas.org) showing the human protein profiles in a large number of normal and cancer tissues as well as a large set of human cell lines...
  75. doi request reprint High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week
    Hanna Tegel
    School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    Biotechnol J 4:51-7. 2009
    ..The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated...
  76. ncbi request reprint Mapping the human proteome using antibodies
    Mathias Uhlen
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, S 106 91 Stockholm, Sweden
    Mol Cell Proteomics 6:1455-6. 2007
  77. pmc A new era for proteomics research?
    Mathias Uhlen
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm SE 10691, Sweden
    Genome Biol 9:325. 2008
    ..A report of the 7th Annual Human Proteome Organization (HUPO) Conference, Amsterdam, the Netherlands, 16-20 August 2008...
  78. doi request reprint Expression and immunogenicity of six putative variable surface proteins in Mycoplasma mycoides subsp. mycoides SC
    Carl Hamsten
    Department of Proteomics, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    Microbiology 154:539-49. 2008
    ..Vmm and one of the putative Vmm-type proteins showed variable expression in vitro...
  79. doi request reprint Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets
    Haakan N Joensson
    Department of Nano Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden
    Angew Chem Int Ed Engl 48:2518-21. 2009
    ..Droplet optical labels allow concurrent analysis of several samples...
  80. doi request reprint Affinity as a tool in life science
    Mathias Uhlen
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Biotechniques 44:649-54. 2008
    ..Recently, such affinity proteomics have allowed the detailed analysis of human protein expression in a comprehensive manner both in normal and disease tissue using tissue microarrays and confocal microscopy...
  81. ncbi request reprint Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats
    Björn Renberg
    School of Biotechnology, Division of Molecular Biotechnology, Royal Institute of Technology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    J Proteome Res 6:171-9. 2007
    ..Finally, the presence of IgA was determined using detection of directly Cy5-labeled normal or IgA-deficient serum samples...
  82. doi request reprint Human Proteinpedia enables sharing of human protein data
    Suresh Mathivanan
    Nat Biotechnol 26:164-7. 2008
  83. ncbi request reprint Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics
    Ann Catrin Andersson
    Department of Genetics and Pathology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    J Histochem Cytochem 54:1413-23. 2006
    ..We conclude that a modified TMA technique, including cell samples, provides a valuable tool for high-throughput analysis of protein expression, and that this technique can be used for global approaches to explore the human proteome...
  84. ncbi request reprint A high-throughput strategy for protein profiling in cell microarrays using automated image analysis
    Sara Strömberg
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Proteomics 7:2142-50. 2007
    ....
  85. ncbi request reprint Large-scale proteomics analysis of human ovarian cancer for biomarkers
    Sofia Bengtsson
    Department of Protein Technology, Lund University, 221 84 Lund, Sweden
    J Proteome Res 6:1440-50. 2007
    ..Currently, we have validated 5 of these antibodies for use in immunohistochemical analysis using tissue microarrays of healthy and diseased ovarian, as well as other, human tissues...
  86. ncbi request reprint A reagent resource to identify proteins and peptides of interest for the cancer community: a workshop report
    Brian B Haab
    Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    Mol Cell Proteomics 5:1996-2007. 2006
    ..This workshop report explores various approaches to develop a framework that will most effectively fulfill the needs of the NCI and the cancer research community...
  87. ncbi request reprint Publication Committee meeting. HUPO 5th annual World Congress. Long Beach, CA, USA 30 October 2006
    Sandra Orchard
    EMBL Outstation European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, UK
    Proteomics 7:1009-11. 2007
    ..Discussions centred on how best to structure interactions between journals, databases and researchers to improve accessibility to data, and facilitate comparisons between datasets...