Jochen M Schwenk

Summary

Affiliation: Royal Institute of Technology
Country: Sweden

Publications

  1. doi request reprint Antibody suspension bead arrays within serum proteomics
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteome Res 7:3168-79. 2008
  2. doi request reprint Comparative protein profiling of serum and plasma using an antibody suspension bead array approach
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    Proteomics 10:532-40. 2010
  3. pmc Autoantibody profiling in multiple sclerosis using arrays of human protein fragments
    Burcu Ayoglu
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 12:2657-72. 2013
  4. doi request reprint Classification of protein profiles from antibody microarrays using heat and detergent treatment
    Anna Häggmark
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    N Biotechnol 29:564-70. 2012
  5. ncbi request reprint A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Microbiol Methods 82:11-8. 2010
  6. doi request reprint Systematic antibody and antigen-based proteomic profiling with microarrays
    Burcu Ayoglu
    Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Expert Rev Mol Diagn 11:219-34. 2011
  7. doi request reprint Validation of serum protein profiles by a dual antibody array approach
    Rebecca Rimini
    Department of Proteomics, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteomics 73:252-66. 2009
  8. ncbi request reprint Antibody-based profiling of cerebrospinal fluid within multiple sclerosis
    Anna Häggmark
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Proteomics 13:2256-67. 2013
  9. ncbi request reprint Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
    Ulrika Qundos
    Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    J Proteomics 95:46-54. 2013
  10. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011

Collaborators

Detail Information

Publications31

  1. doi request reprint Antibody suspension bead arrays within serum proteomics
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteome Res 7:3168-79. 2008
    ..The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins...
  2. doi request reprint Comparative protein profiling of serum and plasma using an antibody suspension bead array approach
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    Proteomics 10:532-40. 2010
    ....
  3. pmc Autoantibody profiling in multiple sclerosis using arrays of human protein fragments
    Burcu Ayoglu
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 12:2657-72. 2013
    ....
  4. doi request reprint Classification of protein profiles from antibody microarrays using heat and detergent treatment
    Anna Häggmark
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    N Biotechnol 29:564-70. 2012
    ..The experimental scheme presented here can be used to systematically categorize antibodies and thereby combine antibodies with similar properties into targeted arrays for analysis of plasma and serum...
  5. ncbi request reprint A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Microbiol Methods 82:11-8. 2010
    ....
  6. doi request reprint Systematic antibody and antigen-based proteomic profiling with microarrays
    Burcu Ayoglu
    Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Expert Rev Mol Diagn 11:219-34. 2011
    ....
  7. doi request reprint Validation of serum protein profiles by a dual antibody array approach
    Rebecca Rimini
    Department of Proteomics, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteomics 73:252-66. 2009
    ....
  8. ncbi request reprint Antibody-based profiling of cerebrospinal fluid within multiple sclerosis
    Anna Häggmark
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Proteomics 13:2256-67. 2013
    ..SERPINA3 instead was detected at higher levels in all MS patients compared to controls. The developed assay procedure now offers new possibilities for broad-scale protein profiling of CSF within neurological disorders. ..
  9. ncbi request reprint Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
    Ulrika Qundos
    Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    J Proteomics 95:46-54. 2013
    ....
  10. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011
    ....
  11. doi request reprint Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment
    Maja Neiman
    Royal Institute of Technology, Solna, Sweden
    J Proteome Res 10:4925-34. 2011
    ....
  12. doi request reprint Validation of affinity reagents using antigen microarrays
    Ronald Sjöberg
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    N Biotechnol 29:555-63. 2012
    ..The presented study illustrates the feasibility of using antigen microarrays for integrative, high-throughput validation of various types of binders and antigens...
  13. pmc Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays
    Jochen M Schwenk
    Science for Life Laboratory, Royal Institute of Technology KTH, Stockholm, Sweden
    Mol Cell Proteomics 9:2497-507. 2010
    ..The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format...
  14. doi request reprint Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP)
    Linn Fagerberg
    Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden
    J Proteome Res 12:2439-48. 2013
    ..The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas ( www.proteinatlas.org )...
  15. pmc Multiplex screening of surface proteins from Mycoplasma mycoides subsp. mycoides small colony for an antigen cocktail enzyme-linked immunosorbent assay
    Maja Neiman
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Clin Vaccine Immunol 16:1665-74. 2009
    ..This set of proteins and technologies therefore offers a powerful combination to drive and further improve serological assays toward reliable, simple, and cost-effective diagnosis of CBPP...
  16. doi request reprint Assessment of antibody specificity using suspension bead arrays
    Jochen M Schwenk
    SciLifeLab Stockholm, KTH Royal Institute of Technology, Tomtebodav, Sweden
    Methods Mol Biol 785:183-9. 2011
    ..While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility...
  17. doi request reprint Highly multiplexed antibody suspension bead arrays for plasma protein profiling
    Kimi Drobin
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Methods Mol Biol 1023:137-45. 2013
    ..Presently, this workflow enables the profiling of 384 samples for up to 384 proteins per assay...
  18. ncbi request reprint Determination of binding specificities in highly multiplexed bead-based assays for antibody proteomics
    Jochen M Schwenk
    Department of Proteomics, Royal Institute of Technology KTH, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 6:125-32. 2007
    ..This high throughput analysis will potentially have great impact on the possibility for the utilization of different antibody proteomics approaches where the quality assessment of antibodies is of the utmost importance...
  19. doi request reprint Antibody suspension bead arrays
    Jochen M Schwenk
    Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, 10691, Stockholm, Sweden
    Methods Mol Biol 723:29-36. 2011
    ..Presently, this workflow enables the profiling of 384 clinical samples for up to 100 proteins per assay...
  20. pmc Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type
    Carl Hamsten
    Department of Proteomics, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 8:2544-54. 2009
    ..In conclusion, a toolbox with recombinant proteins and a flexible suspension array assay that allows multiplex analysis of humoral immune responses to M. mycoides SC has been created...
  21. pmc Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
    Linn Fagerberg
    Science for Life Laboratory, KTH Royal Institute of Technology, SE 171 21 Stockholm, Sweden
    Mol Cell Proteomics 13:397-406. 2014
    ..The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body. ..
  22. doi request reprint Selectivity analysis of single binder assays used in plasma protein profiling
    Maja Neiman
    Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Solna, Sweden
    Proteomics 13:3406-10. 2013
    ..The presented approach will serve as an important tool for resolving differential protein profiles from antibody arrays within plasma biomarker discoveries. ..
  23. doi request reprint Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies
    Johan Rockberg
    School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Mol Oncol 3:238-47. 2009
    ..The work describes a new strategy to develop antibodies directed to non-overlapping epitopes and shows a path of pursuit to explore the epitope space of a target protein...
  24. doi request reprint Magnetic bead-based detection of autoimmune responses using protein microarrays
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    N Biotechnol 26:269-76. 2009
    ..We propose from our findings that the magnetic bead-based detection option provides a simplified and cost effective readout method for protein microarrays...
  25. ncbi request reprint Affinity proteomics within rare diseases: a BIO-NMD study for blood biomarkers of muscular dystrophies
    Burcu Ayoglu
    Affinity Proteomics, SciLifeLab, School of Biotechnology KTH Royal Institute of Technology, Stockholm, Sweden
    EMBO Mol Med 6:918-36. 2014
    ..Using age-matched sub-cohorts, 9 protein profiles correlating with disease progression and severity were identified, which hold promise for the development of new clinical tools for management of dystrophinopathies. ..
  26. ncbi request reprint Magnetic bead assisted labeling of antibodies at nanogram scale
    Mahya Dezfouli
    Science for Life Laboratory, Division of Gene Technology, School of Biotechnology, Royal Institute of Technology KTH, Solna, Sweden
    Proteomics 14:14-8. 2014
    ..With the support of magnetic microspheres, automated labeling of antibodies in parallel using biotin or fluorescent dyes was achieved. ..
  27. ncbi request reprint Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples
    Cecilia Eriksson
    AlbaNova University Center, Department of Biotechnology, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Biotechnol Appl Biochem 56:49-57. 2010
    ..A sample-processing platform like this should be suitable for a number of proteomics technologies; its flexibility in buffer composition allows for different types of downstream applications...
  28. ncbi request reprint Protein microarrays for antibody profiling: specificity and affinity determination on a chip
    Oliver Poetz
    Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
    Proteomics 5:2402-11. 2005
    ..The new microarray based approach has an extraordinary potential to speed up the screening process for the generation of recombinant antibodies with pre-defined selection criteria, since it is intrinsically a high-throughput technology...
  29. ncbi request reprint Protein microarrays: catching the proteome
    Oliver Poetz
    NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr 55, 72770 Reutlingen, Germany
    Mech Ageing Dev 126:161-70. 2005
    ..We will focus on the latest fields of application for the simultaneous determination of a variety of parameters from a minute amount of sample. Future challenges of this cutting-edge technology will be discussed...
  30. ncbi request reprint Protein microarrays: promising tools for proteomic research
    Markus F Templin
    NMI Natural and Medical Sciences Institute, University of Tubingen, Markwiesenstrasse 55, D 72770 Reutlingen, Germany
    Proteomics 3:2155-66. 2003
    ..Microarray technology is an emerging technology used in global analytical approaches and has a considerable impact on proteomic research...
  31. ncbi request reprint Cell microarrays: an emerging technology for the characterization of antibodies
    Jochen M Schwenk
    NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
    Biotechniques . 2002
    ..This new method is applicable for rapidly screening cell surface-specific antibodies with respect to selectivity and cross-reactivity...