Research Topics
Species | Jochen M SchwenkSummaryAffiliation: Royal Institute of Technology Country: Sweden Publications
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Detail Information
Publications
Comparative protein profiling of serum and plasma using an antibody suspension bead array approachJochen M Schwenk
Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
Proteomics 10:532-40. 2010....
Antibody suspension bead arrays within serum proteomicsJochen M Schwenk
Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
J Proteome Res 7:3168-79. 2008..The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins...
Classification of protein profiles from antibody microarrays using heat and detergent treatmentAnna Häggmark
Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
N Biotechnol 29:564-70. 2012..The experimental scheme presented here can be used to systematically categorize antibodies and thereby combine antibodies with similar properties into targeted arrays for analysis of plasma and serum...
Systematic antibody and antigen-based proteomic profiling with microarraysBurcu Ayoglu
Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
Expert Rev Mol Diagn 11:219-34. 2011....
A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serumJesper Gantelius
Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
J Microbiol Methods 82:11-8. 2010....
Validation of serum protein profiles by a dual antibody array approachRebecca Rimini
Department of Proteomics, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
J Proteomics 73:252-66. 2009....
Generation of monospecific antibodies based on affinity capture of polyclonal antibodiesBarbara Hjelm
Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
Protein Sci 20:1824-35. 2011....
Plasma profiling reveals human fibulin-1 as candidate marker for renal impairmentMaja Neiman
Royal Institute of Technology, Solna, Sweden
J Proteome Res 10:4925-34. 2011....
Validation of affinity reagents using antigen microarraysRonald Sjöberg
Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
N Biotechnol 29:555-63. 2012..The presented study illustrates the feasibility of using antigen microarrays for integrative, high-throughput validation of various types of binders and antigens...
Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assaysJochen M Schwenk
Science for Life Laboratory, Royal Institute of Technology KTH, Stockholm, Sweden
Mol Cell Proteomics 9:2497-507. 2010..The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format...
Multiplex screening of surface proteins from Mycoplasma mycoides subsp. mycoides small colony for an antigen cocktail enzyme-linked immunosorbent assayMaja Neiman
Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Clin Vaccine Immunol 16:1665-74. 2009..This set of proteins and technologies therefore offers a powerful combination to drive and further improve serological assays toward reliable, simple, and cost-effective diagnosis of CBPP...
Assessment of antibody specificity using suspension bead arraysJochen M Schwenk
SciLifeLab Stockholm, KTH Royal Institute of Technology, Tomtebodav, Sweden
Methods Mol Biol 785:183-9. 2011..While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility...
Determination of binding specificities in highly multiplexed bead-based assays for antibody proteomicsJochen M Schwenk
Department of Proteomics, Royal Institute of Technology KTH, SE 10691 Stockholm, Sweden
Mol Cell Proteomics 6:125-32. 2007..This high throughput analysis will potentially have great impact on the possibility for the utilization of different antibody proteomics approaches where the quality assessment of antibodies is of the utmost importance...
Antibody suspension bead arraysJochen M Schwenk
Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, 10691, Stockholm, Sweden
Methods Mol Biol 723:29-36. 2011..Presently, this workflow enables the profiling of 384 clinical samples for up to 100 proteins per assay...
Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony typeCarl Hamsten
Department of Proteomics, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Mol Cell Proteomics 8:2544-54. 2009..In conclusion, a toolbox with recombinant proteins and a flexible suspension array assay that allows multiplex analysis of humoral immune responses to M. mycoides SC has been created...
Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodiesJohan Rockberg
School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Mol Oncol 3:238-47. 2009..The work describes a new strategy to develop antibodies directed to non-overlapping epitopes and shows a path of pursuit to explore the epitope space of a target protein...
Magnetic bead-based detection of autoimmune responses using protein microarraysJesper Gantelius
Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
N Biotechnol 26:269-76. 2009..We propose from our findings that the magnetic bead-based detection option provides a simplified and cost effective readout method for protein microarrays...
Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samplesCecilia Eriksson
AlbaNova University Center, Department of Biotechnology, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
Biotechnol Appl Biochem 56:49-57. 2010..A sample-processing platform like this should be suitable for a number of proteomics technologies; its flexibility in buffer composition allows for different types of downstream applications...
Protein microarrays for antibody profiling: specificity and affinity determination on a chipOliver Poetz
, Reutlingen, Germany
Proteomics 5:2402-11. 2005..The new microarray based approach has an extraordinary potential to speed up the screening process for the generation of recombinant antibodies with pre-defined selection criteria, since it is intrinsically a high-throughput technology...
Protein microarrays: catching the proteomeOliver Poetz
, Markwiesenstr. 55, 72770 Reutlingen, Germany
Mech Ageing Dev 126:161-70. 2005..We will focus on the latest fields of application for the simultaneous determination of a variety of parameters from a minute amount of sample. Future challenges of this cutting-edge technology will be discussed...
Protein microarrays: promising tools for proteomic researchMarkus F Templin
NMI Natural and Medical Sciences Institute, , Markwiesenstrasse 55, D-72770 Reutlingen, Germany
Proteomics 3:2155-66. 2003..Microarray technology is an emerging technology used in global analytical approaches and has a considerable impact on proteomic research...
Cell microarrays: an emerging technology for the characterization of antibodiesJochen M Schwenk
, Reutlingen, Germany
Biotechniques . 2002..This new method is applicable for rapidly screening cell surface-specific antibodies with respect to selectivity and cross-reactivity...
