Research Topics
| Emma LundbergSummaryAffiliation: Royal Institute of Technology Country: Sweden Publications
| Collaborators
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Detail Information
Publications
Creation of an antibody-based subcellular protein atlasEmma Lundberg
School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, Sweden
Proteomics 10:3984-96. 2010..The considerations made when creating the subcellular protein atlas and critical parameters of this approach are discussed...- Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopyCharlotte Stadler
Science for Life Laboratory, Royal Institute of Technology, Stockholm, SE 171 65, Sweden
J Proteomics 75:2236-51. 2012..With a large fraction of the human proteome still unexplored, we suggest this approach to be of great importance under the continued work of mapping the human proteome on a subcellular level... - Selection and characterisation of affibody molecules inhibiting the interaction between Ras and Raf in vitroSebastian Grimm
Division of Molecular Biotechnology, Royal Institute of Technology, Stockholm, Sweden
N Biotechnol 27:766-73. 2010..Possible intracellular applications of the selected affibody molecules are discussed... - The correlation between cellular size and protein expression levels--normalization for global protein profilingEmma Lundberg
School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
J Proteomics 71:448-60. 2008..The results demonstrate the value of large-scale, automated proteome analysis using immunohistochemistry, in revealing functional correlations and establishing methods to interpret and mine proteomic data... - Mapping the subcellular protein distribution in three human cell linesLinn Fagerberg
School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, Stockholm, SE 106 91, Sweden
J Proteome Res 10:3766-77. 2011..Our results show distinct organelle proteomes, well conserved across the cell types, and demonstrate that biochemically similar organelles are grouped together... 
Generation of monospecific antibodies based on affinity capture of polyclonal antibodiesBarbara Hjelm
Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
Protein Sci 20:1824-35. 2011....- A single fixation protocol for proteome-wide immunofluorescence localization studiesCharlotte Stadler
School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
J Proteomics 73:1067-78. 2010.... - Defining the transcriptome and proteome in three functionally different human cell linesEmma Lundberg
Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden
Mol Syst Biol 6:450. 2010..Thus, this study shows a path to characterize the transcriptome and proteome in human cells from different origins... - Analysis of transcript and protein overlap in a human osteosarcoma cell lineDaniel Klevebring
Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Royal Institute of Technology, SE 171 65 Solna, Sweden
BMC Genomics 11:684. 2010.... - A genecentric Human Protein Atlas for expression profiles based on antibodiesLisa Berglund
Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
Mol Cell Proteomics 7:2019-27. 2008..The new version of the protein atlas thus is a resource for many areas of biomedical research, including protein science and biomarker discovery... - Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cellsCharlotte Stadler
KTH Royal Institute of Technology, Science for Life Laboratory, School of Biotechnology, Stockholm, Sweden
Nat Methods 10:315-23. 2013..These results show that both IF and FP tagging are reliable techniques and demonstrate the usefulness of an integrative approach for a complete investigation of the subcellular human proteome... - Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domainErik Vernet
Department of Molecular Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, 10691 Stockholm, Sweden
N Biotechnol 25:417-23. 2009..Surface depletion of EGFR led to a dramatic decrease in the amount of kinase domain phosphorylated EGFR, coincident with a significant decrease in the proliferation rate... - Selection and characterization of Affibody ligands to the transcription factor c-JunEmma Lundberg
School of Biotechnology, AlbaNova University Center, Kungliga Tekniska Högskolan, Stockholm, Sweden
Biotechnol Appl Biochem 52:17-27. 2009..The potential intracellular use of Affibody ligands directed against transcription factors and other oncogenic factors is discussed... 
Toward a confocal subcellular atlas of the human proteomeLaurent Barbe
Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
Mol Cell Proteomics 7:499-508. 2008..The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization...- Comparison of total and cytoplasmic mRNA reveals global regulation by nuclear retention and miRNAsBeata Werne Solnestam
KTH Royal Institute of Technology, Science for Life Laboratory SciLifeLab Stockholm, School of Biotechnology, Division of Gene Technology, SE 171 65, Solna, Sweden
BMC Genomics 13:574. 2012..In this study, mRNA fractions from the cytoplasm and from whole cells (total RNA) were prepared from three human cell lines and sequenced using massive parallel sequencing...