Research Topics
| Per BerglundSummaryAffiliation: Royal Institute of Technology Country: Sweden Publications
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Detail Information
Publications
An efficient single-enzymatic cascade for asymmetric synthesis of chiral amines catalyzed by ω-transaminaseBo Wang
KTH Royal Institute of Technology, Division of Biochemistry, School of Biotechnology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Chem Commun (Camb) 49:161-3. 2013....
Engineered enzymes for improved organic synthesisKarl Hult
Department of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Curr Opin Biotechnol 14:395-400. 2003..Both strategies of enzyme engineering can be successful and are very useful for improving the utility of enzymes for applied catalysis. Several examples illustrating these concepts in a variety of enzyme classes have appeared recently...
Direct epoxidation in Candida antarctica lipase B studied by experiment and theoryMaria Svedendahl
Department of Biochemistry, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
Chembiochem 9:2443-51. 2008..The active-site residue His224 functions as a general acid-base catalyst with support from Asp187. Oxyanion stabilization is facilitated by two hydrogen bonds from Thr40...
Fast carbon-carbon bond formation by a promiscuous lipaseMaria Svedendahl
Department of Biochemistry, School of Biotechnology, Royal Institute of Technology (KTH, AlbaNova University Center, SE-106 91 Stockholm, Sweden
J Am Chem Soc 127:17988-9. 2005..The designed enzyme showed high rate enhancements with a catalytic proficiency higher than 108, which is on the same level as that observed for enzymes with native substrates...
Chromobacterium violaceum ω-transaminase variant Trp60Cys shows increased specificity for (S)-1-phenylethylamine and 4'-substituted acetophenones, and follows Swain-Lupton parameterisationKarim Engelmark Cassimjee
KTH Royal Institute of Technology, Division of Biochemistry, School of Biotechnology, AlbaNova University Centre, SE 106 91 Stockholm, Sweden
Org Biomol Chem 10:5466-70. 2012..On comparison with the wild type, reactions of the variant showed increased resonance dependence; this observation together with changed pH optimum and cofactor dependence suggests an altered reaction mechanism...
Crystal structures of the Chromobacterium violaceumω-transaminase reveal major structural rearrangements upon binding of coenzyme PLPMaria S Humble
Division of Biochemistry, KTH Royal Institute of Technology, Stockholm, Sweden
FEBS J 279:779-92. 2012....
Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regenerationKarim Engelmark Cassimjee
Division of Biochemistry, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Centre, SE 106 91 Stockholm, Sweden
Chem Commun (Camb) 46:5569-71. 2010....
Enzyme promiscuity: mechanism and applicationsKarl Hult
School of Biotechnology, Department of Biochemistry, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Trends Biotechnol 25:231-8. 2007..Exploiting enzyme catalytic promiscuity might lead to improvements in existing catalysts and provide novel synthesis pathways that are currently not available...
One-step enzyme extraction and immobilization for biocatalysis applicationsKarim Engelmark Cassimjee
Division of Biochemistry, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
Biotechnol J 6:463-9. 2011....
Silica-immobilized His6-tagged enzyme: alanine racemase in hydrophobic solventKarim Engelmark Cassimjee
Department of Biochemistry, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
Biotechnol Bioeng 99:712-6. 2008..This practical method is a first step towards a general immobilization method for synthesis applications with any enzyme suitable for His6-tagging...
Carbon-carbon bonds by hydrolytic enzymesCecilia Branneby
Department of Biotechnology, KTH, AlbaNova University Center, SE-106 91 Stockholm, Sweden
J Am Chem Soc 125:874-5. 2003..We expect that the new catalytic activity, harbored in the stable protein scaffold of the lipase, will allow aldol additions of substrates, which cannot be reached by traditional aldolases...
Synthesis of cyclic polyamines by enzymatic generation of an amino aldehyde in situKarim Engelmark Cassimjee
School of Biotechnology, AlbaNova University Center, Stockholm, Sweden
Macromol Rapid Commun 33:1580-3. 2012..The enzyme also had activity for 2-amino-propan-1-ol and 2-amino-2-phenyl-ethanol, for which the enantioselectivity was 330 (S) and 32 (R), respectively...
Exploring the active-site of a rationally redesigned lipase for catalysis of Michael-type additionsPeter Carlqvist
Physical Chemistry, Royal Institute of Technology (KTH, 100 44 Stockholm, Sweden
Chembiochem 6:331-6. 2005..The enzyme proficiency was 10(7). Furthermore, the Michael-type reactions followed saturation kinetics and were confirmed to take place in the enzyme active site...
