Helene Andersson-Svahn

Summary

Affiliation: Royal Institute of Technology
Country: Sweden

Publications

  1. doi request reprint Droplet microfluidics--a tool for single-cell analysis
    Haakan N Joensson
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden
    Angew Chem Int Ed Engl 51:12176-92. 2012
  2. doi request reprint A homogeneous assay for protein analysis in droplets by fluorescence polarization
    Haakan N Joensson
    Division of Nanobiotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    Electrophoresis 33:436-9. 2012
  3. doi request reprint Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets
    Haakan N Joensson
    Department of Nano Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden
    Angew Chem Int Ed Engl 48:2518-21. 2009
  4. doi request reprint Droplet size based separation by deterministic lateral displacement-separating droplets by cell--induced shrinking
    Haakan N Joensson
    Div of Nanobiotechnology, Royal Institute of Technology, KTH, AlbaNova University Center, SE 10691 Stockholm, Sweden
    Lab Chip 11:1305-10. 2011
  5. ncbi request reprint Toward a confocal subcellular atlas of the human proteome
    Laurent Barbe
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 7:499-508. 2008
  6. doi request reprint A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Microbiol Methods 82:11-8. 2010
  7. doi request reprint Single-cell culture in microwells
    Sara Lindstrom
    Department of Cell and Molecular Biology, Science for Life Laboratory, Karolinska Institute, Stockholm, Sweden
    Methods Mol Biol 853:41-52. 2012
  8. doi request reprint PCR amplification and genetic analysis in a microwell cell culturing chip
    Sara Lindstrom
    Division of Nanobiotechnology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Lab Chip 9:3465-71. 2009
  9. doi request reprint Magnetic bead-based detection of autoimmune responses using protein microarrays
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    N Biotechnol 26:269-76. 2009
  10. pmc Imaging immune surveillance of individual natural killer cells confined in microwell arrays
    Karolin Guldevall
    Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden
    PLoS ONE 5:e15453. 2010

Collaborators

Detail Information

Publications15

  1. doi request reprint Droplet microfluidics--a tool for single-cell analysis
    Haakan N Joensson
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden
    Angew Chem Int Ed Engl 51:12176-92. 2012
    ..This Review gives an overview of the current state of single-cell analysis involving droplet microfluidics and offers examples where droplet microfluidics can further biological understanding...
  2. doi request reprint A homogeneous assay for protein analysis in droplets by fluorescence polarization
    Haakan N Joensson
    Division of Nanobiotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    Electrophoresis 33:436-9. 2012
    ..This adds to the repertoire of droplet assay techniques a direct protein detection method which can be performed entirely inside droplets without the need for labeling of the analyte molecules...
  3. doi request reprint Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets
    Haakan N Joensson
    Department of Nano Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden
    Angew Chem Int Ed Engl 48:2518-21. 2009
    ..Droplet optical labels allow concurrent analysis of several samples...
  4. doi request reprint Droplet size based separation by deterministic lateral displacement-separating droplets by cell--induced shrinking
    Haakan N Joensson
    Div of Nanobiotechnology, Royal Institute of Technology, KTH, AlbaNova University Center, SE 10691 Stockholm, Sweden
    Lab Chip 11:1305-10. 2011
    ..g. clonal selection. The same device also separates 11 µm from 30 µm droplets at a rate of 12,000 droplets per second, more than twofold faster than previously demonstrated passive hydrodynamic separation devices...
  5. ncbi request reprint Toward a confocal subcellular atlas of the human proteome
    Laurent Barbe
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 7:499-508. 2008
    ..The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization...
  6. doi request reprint A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Microbiol Methods 82:11-8. 2010
    ....
  7. doi request reprint Single-cell culture in microwells
    Sara Lindstrom
    Department of Cell and Molecular Biology, Science for Life Laboratory, Karolinska Institute, Stockholm, Sweden
    Methods Mol Biol 853:41-52. 2012
    ..The glass/silicon plate is compatible with most standard instrumentation to facilitate easy handling and enable use of the plate for single-cell analysis in most laboratory settings...
  8. doi request reprint PCR amplification and genetic analysis in a microwell cell culturing chip
    Sara Lindstrom
    Division of Nanobiotechnology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Lab Chip 9:3465-71. 2009
    ..The presented method of single-cell culture and DNA amplification thus offers a potentially powerful alternative to single-cell PCR, with advantageous robustness and sensitivity...
  9. doi request reprint Magnetic bead-based detection of autoimmune responses using protein microarrays
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    N Biotechnol 26:269-76. 2009
    ..We propose from our findings that the magnetic bead-based detection option provides a simplified and cost effective readout method for protein microarrays...
  10. pmc Imaging immune surveillance of individual natural killer cells confined in microwell arrays
    Karolin Guldevall
    Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden
    PLoS ONE 5:e15453. 2010
    ....
  11. doi request reprint Miniaturization of biological assays -- overview on microwell devices for single-cell analyses
    Sara Lindstrom
    Division of Nanobiotechnology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Biochim Biophys Acta 1810:308-16. 2011
    ..However, cellular heterogeneity implies the importance of studying how individual cells respond, one by one, in order to learn more about drug targeting and cellular behavior...
  12. doi request reprint Engineering and characterization of a bispecific HER2 x EGFR-binding affibody molecule
    Mikaela Friedman
    Division of Molecular Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Biotechnol Appl Biochem 54:121-31. 2009
    ..The potential future use of this and similar constructs, capable of bs targeting of receptors to increase the efficacy and selectivity in imaging and therapy, is discussed...
  13. doi request reprint A microwell array device with integrated microfluidic components for enhanced single-cell analysis
    Sara Lindstrom
    Division of Nanobiotechnology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    Electrophoresis 30:4166-71. 2009
    ....
  14. pmc A lateral flow protein microarray for rapid and sensitive antibody assays
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, 106 91 Stockholm, Sweden
    Int J Mol Sci 12:7748-59. 2011
    ..We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings...
  15. doi request reprint The correlation between cellular size and protein expression levels--normalization for global protein profiling
    Emma Lundberg
    School of Biotechnology, AlbaNova University Center, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    J Proteomics 71:448-60. 2008
    ..The results demonstrate the value of large-scale, automated proteome analysis using immunohistochemistry, in revealing functional correlations and establishing methods to interpret and mine proteomic data...