M Pekny

Summary

Country: Sweden

Publications

  1. pmc Abnormal reaction to central nervous system injury in mice lacking glial fibrillary acidic protein and vimentin
    M Pekny
    Department of Medical Biochemistry, Gothenburg University, SE 405 30 Gothenburg, Sweden
    J Cell Biol 145:503-14. 1999
  2. ncbi request reprint Absence of glial fibrillary acidic protein and vimentin prevents hypertrophy of astrocytic processes and improves post-traumatic regeneration
    Ulrika Wilhelmsson
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, SE 405 30 Goteborg, Sweden
    J Neurosci 24:5016-21. 2004
  3. ncbi request reprint Cytoskeleton and vesicle mobility in astrocytes
    Maja Potokar
    Celica Biomedical Sciences Center, Stegne 21c, SI 1000 Ljubljana, Slovenia
    Traffic 8:12-20. 2007
  4. ncbi request reprint Synemin is expressed in reactive astrocytes in neurotrauma and interacts differentially with vimentin and GFAP intermediate filament networks
    Runfeng Jing
    Department of Cellular Biology and Anatomy and Feist Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    J Cell Sci 120:1267-77. 2007
  5. ncbi request reprint Astrocyte activation and reactive gliosis
    Milos Pekny
    The Arvid Carlsson Institute for Neuroscience, Institute of Clinical Neuroscience, Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    Glia 50:427-34. 2005
  6. ncbi request reprint Intermediate filaments and stress
    Milos Pekny
    Department of Clinical Neuroscience and Rehabilitation, Institute for Neuroscience and Physiology, Sahlgrenska Academy, Goteborg University, Goteborg, Sweden
    Exp Cell Res 313:2244-54. 2007
  7. ncbi request reprint Response to Quinlan and Nilsson: Astroglia sitting at the controls?
    Milos Pekny
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, Medicinaregatan 9A, SE 413 90 Goteborg, Sweden
    Trends Neurosci 27:243-4. 2004
  8. ncbi request reprint Astrocyte intermediate filaments in CNS pathologies and regeneration
    Milos Pekny
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, Box 430, 405 30 Goteborg, Sweden
    J Pathol 204:428-37. 2004
  9. ncbi request reprint GFAP-deficient astrocytes are capable of stellation in vitro when cocultured with neurons and exhibit a reduced amount of intermediate filaments and an increased cell saturation density
    M Pekny
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Exp Cell Res 239:332-43. 1998
  10. ncbi request reprint The impact of genetic removal of GFAP and/or vimentin on glutamine levels and transport of glucose and ascorbate in astrocytes
    M Pekny
    Department of Medical Biochemistry, University of Gothenburg, Sweden
    Neurochem Res 24:1357-62. 1999

Collaborators

Detail Information

Publications43

  1. pmc Abnormal reaction to central nervous system injury in mice lacking glial fibrillary acidic protein and vimentin
    M Pekny
    Department of Medical Biochemistry, Gothenburg University, SE 405 30 Gothenburg, Sweden
    J Cell Biol 145:503-14. 1999
    ..These results show that GFAP and vimentin are required for proper glial scar formation in the injured central nervous system and that some degree of functional overlap exists between these IF proteins...
  2. ncbi request reprint Absence of glial fibrillary acidic protein and vimentin prevents hypertrophy of astrocytic processes and improves post-traumatic regeneration
    Ulrika Wilhelmsson
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, SE 405 30 Goteborg, Sweden
    J Neurosci 24:5016-21. 2004
    ..These findings show a novel role for intermediate filaments in astrocytes and implicate reactive astrocytes as potent inhibitors of neuroregeneration...
  3. ncbi request reprint Cytoskeleton and vesicle mobility in astrocytes
    Maja Potokar
    Celica Biomedical Sciences Center, Stegne 21c, SI 1000 Ljubljana, Slovenia
    Traffic 8:12-20. 2007
    ..Depolymerization of intermediate filaments strongly affected vesicle trafficking and in their absence the fraction of vesicles with directional mobility was reduced...
  4. ncbi request reprint Synemin is expressed in reactive astrocytes in neurotrauma and interacts differentially with vimentin and GFAP intermediate filament networks
    Runfeng Jing
    Department of Cellular Biology and Anatomy and Feist Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    J Cell Sci 120:1267-77. 2007
    ..In astrocytes, this partner is vimentin and not GFAP, which implies a functional difference between these two type III IF proteins...
  5. ncbi request reprint Astrocyte activation and reactive gliosis
    Milos Pekny
    The Arvid Carlsson Institute for Neuroscience, Institute of Clinical Neuroscience, Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    Glia 50:427-34. 2005
    ....
  6. ncbi request reprint Intermediate filaments and stress
    Milos Pekny
    Department of Clinical Neuroscience and Rehabilitation, Institute for Neuroscience and Physiology, Sahlgrenska Academy, Goteborg University, Goteborg, Sweden
    Exp Cell Res 313:2244-54. 2007
    ..We discuss selected situations in which responses to stress are clearly influenced by intermediate filaments...
  7. ncbi request reprint Response to Quinlan and Nilsson: Astroglia sitting at the controls?
    Milos Pekny
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, Medicinaregatan 9A, SE 413 90 Goteborg, Sweden
    Trends Neurosci 27:243-4. 2004
  8. ncbi request reprint Astrocyte intermediate filaments in CNS pathologies and regeneration
    Milos Pekny
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, Box 430, 405 30 Goteborg, Sweden
    J Pathol 204:428-37. 2004
    ..GFAP is the principal astrocyte intermediate filament protein and dominant mutations in the GFAP gene have been shown to lead to Alexander disease, a fatal neurodegenerative condition in humans...
  9. ncbi request reprint GFAP-deficient astrocytes are capable of stellation in vitro when cocultured with neurons and exhibit a reduced amount of intermediate filaments and an increased cell saturation density
    M Pekny
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Exp Cell Res 239:332-43. 1998
    ..The latter leads us to speculate that the loss of GFAP expression observed focally in a proportion of human malignant gliomas may reflect tumor progression toward a more rapidly growing and malignant phenotype...
  10. ncbi request reprint The impact of genetic removal of GFAP and/or vimentin on glutamine levels and transport of glucose and ascorbate in astrocytes
    M Pekny
    Department of Medical Biochemistry, University of Gothenburg, Sweden
    Neurochem Res 24:1357-62. 1999
    ..Furthermore, the effect of GFAP is dose-dependent since the glutamine concentration in GFAP+/- astrocytes falls between those in wild-type and GFAP-/- astrocytes...
  11. ncbi request reprint Intermediate filament protein partnership in astrocytes
    C Eliasson
    Department of Medical Biochemistry, University of Gothenburg, SE 405 30 Gothenburg, Sweden
    J Biol Chem 274:23996-4006. 1999
    ..Here we present models by which the normal and pathogenic functions of intermediate filaments may be elucidated in astrocytes...
  12. ncbi request reprint Altered taurine release following hypotonic stress in astrocytes from mice deficient for GFAP and vimentin
    M Ding
    Department of Anatomy and Cell Biology, University of Goteborg, PO Box 420, SE 405 30, Goteborg, Sweden
    Brain Res Mol Brain Res 62:77-81. 1998
    ..The results strengthen the role of the cytoskeleton in astrocyte volume regulation and suggest an involvement of intermediate filaments in the process...
  13. ncbi request reprint Paracrine PDGF-B/PDGF-Rbeta signaling controls mesangial cell development in kidney glomeruli
    P Lindahl
    Department of Medical Biochemistry, Goteborg University, PO Box 440, SE 405 30 Goteborg, Sweden
    Development 125:3313-22. 1998
    ....
  14. pmc Mice lacking glial fibrillary acidic protein display astrocytes devoid of intermediate filaments but develop and reproduce normally
    M Pekny
    Department of Medical Biochemistry, University of Goteborg, Sweden
    EMBO J 14:1590-8. 1995
    ..The GFAP-negative mice displayed post-traumatic reactive gliosis, which suggests that GFAP up-regulation, a hallmark of reactive gliosis, is not an obligatory requirement for this process...
  15. ncbi request reprint PDGF-A signaling is a critical event in lung alveolar myofibroblast development and alveogenesis
    H Bostrom
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Cell 85:863-73. 1996
    ..The two PDGF null phenotypes also reveal analogous morphogenetic functions for myofibroblast-type cells in lung and kidney organogenesis...
  16. ncbi request reprint Mice deficient for the complement factor B develop and reproduce normally
    M Pekna
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Scand J Immunol 47:375-80. 1998
    ..This model enables the dissection of the complement cascade in vivo and the elucidation of the relative contribution of this complement pathway in the various physiological and pathological phenomena ascribed to the complement system...
  17. ncbi request reprint Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice
    Asa Widestrand
    Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, Goteborg University, Medicinaregatan 9A, SE 413 90 Goteborg, Sweden
    Stem Cells 25:2619-27. 2007
    ..Thus, reactive gliosis may adversely affect the integration of transplanted neural progenitor cells in the brain. Disclosure of potential conflicts of interest is found at the end of this article...
  18. ncbi request reprint Targeted disruption of the murine gene coding for the third complement component (C3)
    M Pekna
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Scand J Immunol 47:25-9. 1998
    ..The C3-deficient mice should be valuable for the study of the roles of the complement system in vivo in a variety of physiological and pathological situations...
  19. ncbi request reprint Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities
    P Leveen
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Genes Dev 8:1875-87. 1994
    ..Their hematological status includes erythroblastosis, macrocytic anemia, and thrombocytopenia. On the basis of these findings, we conclude that PDGF B has crucial roles in vivo in establishing certain renal and circulatory functions...
  20. ncbi request reprint 14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics
    Carina Sihlbom
    Center for Brain Repair and Rehabilitation CBR, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Institute of Biomedicine, Sahlgrenska Academy, Goteborg University, Sweden
    J Proteome Res 6:3491-500. 2007
    ....
  21. ncbi request reprint Under stress, the absence of intermediate filaments from Müller cells in the retina has structural and functional consequences
    Andrea Lundkvist
    Department of Medical Biochemistry, The Sahlgrenska Academy at Goteborg University, Medicinaregatan 9C, SE 41390 Göteborg, Sweden
    J Cell Sci 117:3481-8. 2004
    ..Furthermore, the absence of IFs in Müller cells leads to an abnormal response of the vascular system to ischemia, specifically decreased ability of newly formed blood vessels to traverse the ILM...
  22. ncbi request reprint The role of astrocytes and complement system in neural plasticity
    Milos Pekny
    Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute for Neuroscience and Physiology at Sahlgrenska Academy Göteborg University, 405 30 Goteborg, Sweden
    Int Rev Neurobiol 82:95-111. 2007
    ..However, growing body of evidence suggests that complement may be a physiological neuroprotective mechanism as well as it may participate in maintenance and repair of the adult brain...
  23. ncbi request reprint Increased insulin secretion and glucose tolerance in mice lacking islet amyloid polypeptide (amylin)
    S Gebre-Medhin
    Department of Medical Biochemistry, Goteborg University, Sweden
    Biochem Biophys Res Commun 250:271-7. 1998
    ....
  24. doi request reprint Retinoids and activation of PKC induce tissue-type plasminogen activator expression and storage in human astrocytes
    K Hultman
    Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation, the Sahlgrenska Academy at University of Gothenburg, Department of Clinical Genetics, Sahlgrenska University Hospital, Gothenburg, Sweden
    J Thromb Haemost 6:1796-803. 2008
    ..In contrast to endothelial cells, little is known about the regulation of t-PA gene expression and secretion in astrocytes...
  25. ncbi request reprint Signaling through C5aR is not involved in basal neurogenesis
    Yalda Rahpeymai Bogestål
    Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    J Neurosci Res 85:2892-7. 2007
    ..Our results indicate that signaling through C5aR is not involved in basal neurogenesis in adult mice and that basal neurogenesis in adult C3a/GFAP and C5a/GFAP mice is not altered...
  26. ncbi request reprint Protective role of reactive astrocytes in brain ischemia
    Lizhen Li
    Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology at Sahlgrenska Academy, Goteborg University, Goteborg, Sweden
    J Cereb Blood Flow Metab 28:468-81. 2008
    ..Thus, reactive astrocytes have a protective role in brain ischemia, and the absence of astrocyte IFs is linked to changes in glutamate transport, ET(B)R-mediated control of gap junctions, and PAI-1 expression...
  27. doi request reprint Expression of plasminogen activator inhibitor-1 and protease nexin-1 in human astrocytes: Response to injury-related factors
    Karin Hultman
    Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden
    J Neurosci Res 88:2441-9. 2010
    ..We show here that human astrocytes express PAI-1 and PN-1 and demonstrate that this astrocytic expression is regulated in a dynamic manner by injury-related factors...
  28. ncbi request reprint Increased cell proliferation and neurogenesis in the hippocampal dentate gyrus of old GFAP(-/-)Vim(-/-) mice
    Asa Larsson
    Department of Medical Biochemistry, Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    Neurochem Res 29:2069-73. 2004
    ..These findings suggest that the adult hippocampal neurogenesis in healthy old mice can be increased by modulating astrocyte reactivity...
  29. ncbi request reprint Neuron-specific ablation of PDGF-B is compatible with normal central nervous system development and astroglial response to injury
    Maria Enge
    Department of Medical Biochemistry, Goteborg University, Goteborg, Sweden
    Neurochem Res 28:271-9. 2003
    ..Alternatively, other and more specific analyses of CNS functions in the normal and injured states will be required to demonstrate such a role...
  30. ncbi request reprint Enriched environment and astrocytes in central nervous system regeneration
    Michael Nilsson
    Center for Brain Repair and Rehabilitation CBR, Department of Clinical Neuroscience and Rehabilitation, Institute for Neuroscience and Physiology, Sahlgrenska Academy, Goteborg University, Goteborg, Sweden
    J Rehabil Med 39:345-52. 2007
    ..It also summarizes research showing that astrocytes - considered only years ago merely to nurse and support neurones - are a novel and highly interesting target for regenerative strategies in the brain and spinal cord...
  31. pmc Redefining the concept of reactive astrocytes as cells that remain within their unique domains upon reaction to injury
    Ulrika Wilhelmsson
    Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, Goteborg University, SE 405 30 Goteborg, Sweden
    Proc Natl Acad Sci U S A 103:17513-8. 2006
    ..This work helps to redefine the century-old concept of hypertrophy of reactive astrocytes...
  32. doi request reprint Complement-derived anaphylatoxin C3a regulates in vitro differentiation and migration of neural progenitor cells
    Noriko Shinjyo
    Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, SE 405 30 Gothenburg, Sweden
    Stem Cells 27:2824-32. 2009
    ..Our results provide the first evidence that C3a regulates neurogenesis by directly affecting the fate and properties of NPCs...
  33. pmc Complement: a novel factor in basal and ischemia-induced neurogenesis
    Yalda Rahpeymai
    Institute of Biomedicine, Department of Medical Chemistry and Cell Biology, Sahlgrenska Academy, Goteborg University, Goteborg, Sweden
    EMBO J 25:1364-74. 2006
    ..Thus, in the adult mammalian CNS, complement activation products promote both basal and ischemia-induced neurogenesis...
  34. ncbi request reprint Loss of GFAP expression in high-grade astrocytomas does not contribute to tumor development or progression
    Ulrika Wilhelmsson
    Department of Medical Biochemistry, Goteborg University, Box 440, SE 405 30 Goteborg, Sweden
    Oncogene 22:3407-11. 2003
    ..Thus, the loss of GFAP expression frequently seen in high-grade astrocytomas does not constitute a step in tumor development. Rather, it may represent the undifferentiated state of these cells...
  35. pmc Re-establishing the regenerative potential of central nervous system axons in postnatal mice
    Kin Sang Cho
    Schepens Eye Research Institute, Program in Neuroscience and Department of Ophthalmology, Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USA
    J Cell Sci 118:863-72. 2005
    ..These results suggest that an early postnatal downregulation of Bcl-2 and post-traumatic reactive gliosis are two important elements of axon regenerative failure in the CNS...
  36. pmc Abnormal reactivity of muller cells after retinal detachment in mice deficient in GFAP and vimentin
    Mark R Verardo
    Neuroscience Research Institute, University of California, Santa Barbara, California 93106, USA
    Invest Ophthalmol Vis Sci 49:3659-65. 2008
    ..To determine the roles of glial fibrillary acidic protein (GFAP) and vimentin in Müller cell reactivity...
  37. ncbi request reprint Robust neural integration from retinal transplants in mice deficient in GFAP and vimentin
    Reiko Kinouchi
    Schepens Eye Research Institute and Department of Ophthalmology, Program in Neuroscience, Harvard Medical School, 20 Staniford Street, Boston, Massachusetts 02114 USA
    Nat Neurosci 6:863-8. 2003
    ..Our results indicate an essential role for reactive astroglial cells in preventing neural graft integration after transplantation...
  38. ncbi request reprint Bfsp2 mutation found in mouse 129 strains causes the loss of CP49' and induces vimentin-dependent changes in the lens fibre cell cytoskeleton
    Aileen Sandilands
    Department of Molecular and Cellular Pathology, University of Dundee, Dundee DD1 5EH Scotland, UK
    Exp Eye Res 78:875-89. 2004
    ..This report of a natural mutation in Bfsp2 in the 129 and other mouse strains also has important implications for lens studies that have used the 129X1/SvJ strain in knockout strategies...
  39. ncbi request reprint Dynamics of mutated GFAP aggregates revealed by real-time imaging of an astrocyte model of Alexander disease
    Cyril Mignot
    INSERM UMR S 546, Paris, France
    Exp Cell Res 313:2766-79. 2007
    ..Particularly, vimentin displayed an anti-aggregation effect on mutated GFAP. Our data indicate a dynamic and reversible aggregation of mutated GFAP, suggesting that therapeutic approaches may be possible...
  40. ncbi request reprint Stress models for the study of intermediate filament function
    E Birgitte Lane
    Cancer Research UK, Cell Structure Research Group, University of Dundee School of Life Sciences, Dundee DD1 5EH, Scotland
    Methods Cell Biol 78:229-64. 2004
  41. pmc Attenuated glial reactions and photoreceptor degeneration after retinal detachment in mice deficient in glial fibrillary acidic protein and vimentin
    Toru Nakazawa
    Angiogenesis Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA
    Invest Ophthalmol Vis Sci 48:2760-8. 2007
    ....
  42. ncbi request reprint Bfsp2 mutation found in mouse 129 strains causes the loss of CP49 and induces vimentin-dependent changes in the lens fibre cell cytoskeleton
    Aileen Sandilands
    Department of Molecular and Cellular Pathology, University of Dundee DD1 5EH, Scotland, UK
    Exp Eye Res 78:109-23. 2004
    ..This report of a natural mutation in Bfsp2 in the 129 and other mouse strains also has important implications for lens studies that have used the 129X1/SvJ strain in knockout strategies...
  43. ncbi request reprint Mice lacking the adenosine A1 receptor are anxious and aggressive, but are normal learners with reduced muscle strength and survival rate
    Lydia Gimenez-Llort
    Medical Psychology Unit, Department of Psychiatry and Forensic Medicine, Neuroscience Institute, Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain
    Eur J Neurosci 16:547-50. 2002
    ..These results confirm the involvement of adenosine in motor activity, exploratory behaviour, anxiety and aggressiveness. A1Rs also appear to play a critical role in ageing-related deterioration...