M Unemo

Summary

Affiliation: Orebro University Hospital
Country: Sweden

Publications

  1. doi request reprint Emergence, spread and characteristics of Neisseria gonorrhoeae isolates with in vitro decreased susceptibility and resistance to extended-spectrum cephalosporins in Sweden
    Daniel Golparian
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Sex Transm Infect 86:454-60. 2010
  2. doi request reprint Guidelines for the laboratory diagnosis of mycoplasma genitalium infections in East European countries
    Elena Shipitsyna
    Microbiology Laboratory, DO Ott Research Institute of Obstetrics and Gynecology, Pavlov State Medical University, St Petersburg, Russia
    Acta Derm Venereol 90:461-7. 2010
  3. doi request reprint Antimicrobial susceptibility/resistance and genetic characteristics of Neisseria gonorrhoeae isolates from Poland, 2010-2012
    Beata Mlynarczyk-Bonikowska
    WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    BMC Infect Dis 14:65. 2014
  4. doi request reprint Does the '2012 IUSTI ECCG report on the diagnosis and management of Neisseria gonorrhoeae infections in Europe' depict the situation in Europe?
    M Unemo
    World Health Organization Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, Orebro University Hospital, Orebro, Sweden
    Int J STD AIDS 24:423-6. 2013
  5. pmc Composition of the vaginal microbiota in women of reproductive age--sensitive and specific molecular diagnosis of bacterial vaginosis is possible?
    Elena Shipitsyna
    Laboratory of Microbiology, D O Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia
    PLoS ONE 8:e60670. 2013
  6. pmc Neisseria gonorrhoeae strain with high-level resistance to spectinomycin due to a novel resistance mechanism (mutated ribosomal protein S5) verified in Norway
    Magnus Unemo
    WHO Collaborating Centre for Gonorrhoea and Other STIs, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 57:1057-61. 2013
  7. pmc Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from Vietnam, 2011
    Birgitta Olsen
    School of Health and Medical Sciences, Orebro University, Orebro, Sweden
    BMC Infect Dis 13:40. 2013
  8. pmc Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from India, Pakistan and Bhutan in 2007-2011
    Sunil Sethi
    Department of Medical Microbiology, Post Graduate Institute of MedicalEducation and Research, Chandigarh, India
    BMC Infect Dis 13:35. 2013
  9. pmc Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea
    Magnus Unemo
    WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Future Microbiol 7:1401-22. 2012
  10. pmc In vitro activity of the new fluoroketolide solithromycin (CEM-101) against a large collection of clinical Neisseria gonorrhoeae isolates and international reference strains, including those with high-level antimicrobial resistance: potential treatment op
    Daniel Golparian
    WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 56:2739-42. 2012

Detail Information

Publications82

  1. doi request reprint Emergence, spread and characteristics of Neisseria gonorrhoeae isolates with in vitro decreased susceptibility and resistance to extended-spectrum cephalosporins in Sweden
    Daniel Golparian
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Sex Transm Infect 86:454-60. 2010
    ....
  2. doi request reprint Guidelines for the laboratory diagnosis of mycoplasma genitalium infections in East European countries
    Elena Shipitsyna
    Microbiology Laboratory, DO Ott Research Institute of Obstetrics and Gynecology, Pavlov State Medical University, St Petersburg, Russia
    Acta Derm Venereol 90:461-7. 2010
    ....
  3. doi request reprint Antimicrobial susceptibility/resistance and genetic characteristics of Neisseria gonorrhoeae isolates from Poland, 2010-2012
    Beata Mlynarczyk-Bonikowska
    WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    BMC Infect Dis 14:65. 2014
    ..gonorrhoeae isolates in Poland in 2010-2012...
  4. doi request reprint Does the '2012 IUSTI ECCG report on the diagnosis and management of Neisseria gonorrhoeae infections in Europe' depict the situation in Europe?
    M Unemo
    World Health Organization Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, Orebro University Hospital, Orebro, Sweden
    Int J STD AIDS 24:423-6. 2013
    ..This, together with increased access to and use of antimicrobial susceptibility testing, is crucial in controlling the emergent spread of cephalosporin-resistant and multidrug-resistant gonorrhoea...
  5. pmc Composition of the vaginal microbiota in women of reproductive age--sensitive and specific molecular diagnosis of bacterial vaginosis is possible?
    Elena Shipitsyna
    Laboratory of Microbiology, D O Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia
    PLoS ONE 8:e60670. 2013
    ..This study aimed to investigate the composition of the vaginal microbiota in women of reproductive age (healthy women and women with BV), with the view of developing molecular criteria for BV diagnosis...
  6. pmc Neisseria gonorrhoeae strain with high-level resistance to spectinomycin due to a novel resistance mechanism (mutated ribosomal protein S5) verified in Norway
    Magnus Unemo
    WHO Collaborating Centre for Gonorrhoea and Other STIs, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 57:1057-61. 2013
    ..The traditional spectinomycin resistance gene (16S rRNA) was wild type. Despite this exceedingly rare finding, spectinomycin available for treatment of ceftriaxone-resistant urogenital gonorrhea would be very valuable...
  7. pmc Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from Vietnam, 2011
    Birgitta Olsen
    School of Health and Medical Sciences, Orebro University, Orebro, Sweden
    BMC Infect Dis 13:40. 2013
    ..gonorrhoeae isolated in 2011 in Hanoi, Vietnam...
  8. pmc Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from India, Pakistan and Bhutan in 2007-2011
    Sunil Sethi
    Department of Medical Microbiology, Post Graduate Institute of MedicalEducation and Research, Chandigarh, India
    BMC Infect Dis 13:35. 2013
    ..In this paper, we describe the prevalence of antimicrobial resistance and molecular characteristics of N. gonorrhoeae isolates from India, Pakistan, and Bhutan in 2007-2011...
  9. pmc Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea
    Magnus Unemo
    WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Future Microbiol 7:1401-22. 2012
    ..Novel treatment strategies, antimicrobials (or other compounds) and, ideally, a vaccine must be developed...
  10. pmc In vitro activity of the new fluoroketolide solithromycin (CEM-101) against a large collection of clinical Neisseria gonorrhoeae isolates and international reference strains, including those with high-level antimicrobial resistance: potential treatment op
    Daniel Golparian
    WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 56:2739-42. 2012
    ..The activity of solithromycin was mainly superior to that of other antimicrobials (n = 10) currently or previously recommended for gonorrhea treatment. Solithromycin might be an effective treatment option for gonorrhea...
  11. doi request reprint Antibiotic resistance in Neisseria gonorrhoeae: origin, evolution, and lessons learned for the future
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Ann N Y Acad Sci 1230:E19-28. 2011
    ..gonorrhoeae, the linkage between resistance and biological fitness of N. gonorrhoeae, lessons learned, and future perspectives are reviewed and discussed...
  12. pmc High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure
    Magnus Unemo
    WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 56:1273-80. 2012
    ..Nevertheless, new treatment strategies and/or drugs and, ideally, a vaccine are essential to develop for efficacious gonorrhea management...
  13. pmc HIV, STI prevalence and risk behaviours among women selling sex in Lahore, Pakistan
    Mohsin Saeed Khan
    Mohsin Saeed Khan, Division of Global Health IHCAR, Department of Public Health Sciences, Karolinska Institutet, Stockholm, Sweden
    BMC Infect Dis 11:119. 2011
    ..The aim of our study was to estimate the prevalence of HIV and STIs, and assess knowledge and risk behaviours related to HIV/STI, among women selling sex in Lahore, Pakistan...
  14. ncbi request reprint Neisseria gonorrhoeae population in Arkhangelsk, Russia: phenotypic and genotypic heterogeneity
    M Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Clin Microbiol Infect 13:873-8. 2007
    ..Thorough knowledge concerning the incidence of gonorrhoea, antibiotic susceptibility and other phenotypic and genotypic characteristics of the N. gonorrhoeae strains circulating in eastern Europe is crucial...
  15. pmc Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strain
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    APMIS 115:231-41. 2007
    ..gonorrhoeae in the effective treatment of gonorrhoea...
  16. pmc The Swedish new variant of Chlamydia trachomatis: genome sequence, morphology, cell tropism and phenotypic characterization
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Orebro University Hospital, Orebro, Sweden
    Microbiology 156:1394-404. 2010
    ..Therefore, the rapid transmission of nvCT in Sweden was due to the strong diagnostic selective advantage and its introduction into a high-frequency transmitting population...
  17. doi request reprint Recommended antimicrobial treatment of uncomplicated gonorrhoea in 2009 in 11 East European countries: implementation of a Neisseria gonorrhoeae antimicrobial susceptibility programme in this region is crucial
    M Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro SE 701 85, Sweden
    Sex Transm Infect 86:442-4. 2010
    ..Quality-assured and quality-controlled AMR surveillance data on gonococci globally are crucial for public health purposes. In East European countries, knowledge regarding gonococcal AMR and its prevalence is limited...
  18. ncbi request reprint Pulsed-field gel electrophoresis as an epidemiologic tool for Neisseria gonorrhoeae: identification of clusters within serovars
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria and Unit for Infectious Disease Control, Department of Clinical Microbiology and Immunology, Orebro Medical Centre Hospital, Orebro, Sweden
    Sex Transm Dis 29:25-31. 2002
    ..The increasing incidence of gonorrhea in Sweden in 1998 was due to mostly domestic cases. Among these, two core groups were identified: homosexual men with serovar IB-2 and young heterosexuals with serovar IB-3...
  19. doi request reprint Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes
    Magnus Unemo
    Department of Clinical Microbiology, Orebro University Hospital, Sweden
    J Antimicrob Chemother 63:1142-51. 2009
    ..Here we phenotypically and genetically characterized the 2008 WHO N. gonorrhoeae reference panel, widely used under existing WHO AMR surveillance protocols...
  20. ncbi request reprint Genetic homogeneity/heterogeneity of Propionibacterium acnes isolated from patients during cardiothoracic reoperation
    Magnus Unemo
    Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Anaerobe 13:121-6. 2007
    ..Existence of several clonally related P. acnes isolates derived from multiple samples from patients suffering from deep SWI after cardiothoracic surgery has not previously been shown...
  21. pmc Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategies
    M Unemo
    Department of Clinical Microbiology, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Sex Transm Infect 83:47-51. 2007
    ..This enzyme has previously been considered to be almost universally present in N gonorrhoeae. However, increasing numbers of N gonorrhoeae isolates lacking PIP activity have been identified...
  22. doi request reprint The Swedish new variant of Chlamydia trachomatis
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Curr Opin Infect Dis 24:62-9. 2011
    ..This information provides an interesting insight into the emergence of new strains (how, where, and when), and the important lessons learned are discussed...
  23. ncbi request reprint Experiences with the new genetic variant of Chlamydia trachomatis in Orebro county, Sweden - proportion, characteristics and effective diagnostic solution in an emergent situation
    M Unemo
    Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Euro Surveill 12:E5-6. 2007
    ..LightMix 480HT seemed sensitive, specific, and enabled high throughput diagnostics. However, rare low positive samples may be false-negative. Frequent surveillance and evaluations of diagnostic methods worldwide are crucial...
  24. pmc Laboratory diagnosis of Neisseria gonorrhoeae in St Petersburg, Russia: inventory, performance characteristics and recommended optimisations
    M Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Sex Transm Infect 82:41-4. 2006
    ....
  25. pmc Comparison of serologic and genetic porB-based typing of Neisseria gonorrhoeae: consequences for future characterization
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    J Clin Microbiol 41:4141-7. 2003
    ..In conclusion, analysis of short highly variable regions of the porB gene could form the basis for a fast molecular epidemiological tool for the examination of emergence and transmission of N. gonorrhoeae strains within the community...
  26. doi request reprint Gonorrhoea surveillance, laboratory diagnosis and antimicrobial susceptibility testing of Neisseria gonorrhoeae in 11 countries of the eastern part of the WHO European region
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Sweden
    APMIS 119:643-9. 2011
    ..The information provided herein will be useful in this respect...
  27. pmc Molecular typing of Neisseria gonorrhoeae isolates by pyrosequencing of highly polymorphic segments of the porB gene
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    J Clin Microbiol 42:2926-34. 2004
    ..In conclusion, the fast and high-throughput pyrosequencing technology can be used for molecular epidemiological characterization of N. gonorrhoeae...
  28. ncbi request reprint The porA pseudogene of Neisseria gonorrhoeae--low level of genetic polymorphism and a few, mainly identical, inactivating mutations
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, Orebro, Sweden
    APMIS 113:410-9. 2005
    ..The highly conserved N. gonorrhoeae porA pseudogene may reflect an evolutionary neutral molecular clock and may be a suitable genetic target for diagnosis of N. gonorrhoeae...
  29. doi request reprint Evaluation of six nucleic acid amplification tests used for diagnosis of Neisseria gonorrhoeae in Russia compared with an international strictly validated real-time porA pseudogene polymerase chain reaction
    E Shipitsyna
    Laboratory of Microbiology, D O Ott Research Institute of Obstetrics and Gynaecology, St Petersburg, Russia
    J Eur Acad Dermatol Venereol 23:1246-53. 2009
    ..However, these NAATs have never been validated to any international well-recognized diagnostic NAAT...
  30. doi request reprint First evaluation of six nucleic acid amplification tests widely used in the diagnosis of Chlamydia trachomatis in Russia
    E Shipitsyna
    Laboratory of Microbiology, D O Ott Research Institute of Obstetrics and Gynaecology, St Petersburg, Russia
    J Eur Acad Dermatol Venereol 23:268-76. 2009
    ..In Russia, nationally developed nucleic acid amplification tests (NAATs), which have never been validated to international commercially available NAATs, are mainly used in the diagnosis of Chlamydia trachomatis infection...
  31. doi request reprint Sexual behaviours, knowledge and attitudes regarding safe sex, and prevalence of non-viral sexually transmitted infections among attendees of youth clinics in St. Petersburg, Russia
    E Shipitsyna
    Laboratory of Microbiology, DO Ott Research Institute of Obstetrics and Gynaecology, St Petersburg, Russia
    J Eur Acad Dermatol Venereol 27:e75-84. 2013
    ..Knowledge of STI prevalence and risk factors are essential tools to elaborate preventive strategies. However, internationally reported studies on epidemiology of STIs among the youth in Russia are mainly lacking...
  32. doi request reprint In vitro susceptibility to 17 antimicrobials of clinical Clostridium difficile isolates collected in 1993-2007 in Sweden
    T Noren
    Department of Infectious Diseases, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Clin Microbiol Infect 16:1104-10. 2010
    ..difficile isolates showed variations of multiresistance to be due to a specific PCR ribotype 012, emphasizing the importance of genotyping when evaluating emerging resistance over time...
  33. doi request reprint In vitro antimicrobial synergy testing of coagulase-negative staphylococci isolated from prosthetic joint infections using Etest and with a focus on rifampicin and linezolid
    B Hellmark
    Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, 701 85 Orebro, Sweden
    Eur J Clin Microbiol Infect Dis 29:591-5. 2010
    ..In conclusion, Etest is an objective and easily performed in vitro method for antimicrobial synergy testing. However, each isolate requires testing for the specific combination considered for treatment...
  34. doi request reprint Staphylococcal cassette chromosome mec (SCCmec) and arginine catabolic mobile element (ACME) in Staphylococcus epidermidis isolated from prosthetic joint infections
    B Hellmark
    Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, 701 85 Orebro, Sweden
    Eur J Clin Microbiol Infect Dis 32:691-7. 2013
    ..ACME was more common among the commensal isolates and may represent a survival benefit for S. epidermidis colonising healthy individuals in the community...
  35. doi request reprint First evaluation of polymerase chain reaction assays used for diagnosis of Mycoplasma genitalium in Russia
    E Shipitsyna
    Laboratory of Microbiology, D O Ott Research Institute of Obstetrics and Gynaecology, St Petersburg, Russia
    J Eur Acad Dermatol Venereol 23:1164-72. 2009
    ..In Russia, several M. genitalium polymerase chain reaction (PCR) assays have been developed; however, any evaluation of their performance has never been performed...
  36. doi request reprint Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections with special focus on rifampicin and variability of the rpoB gene
    B Hellmark
    Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Clin Microbiol Infect 15:238-44. 2009
    ..Detection of mecA is necessary before initiating treatment of infections due to S. epidermidis when it displays intermediate susceptibility to cefoxitin...
  37. ncbi request reprint Molecular epidemiology of Neisseria gonorrhoeae- identification of the first presumed Swedish transmission chain of an azithromycin-resistant strain
    David Lundbäck
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Sweden
    APMIS 114:67-71. 2006
    ..gonorrhoeae in order to identify emergence of new resistance, monitor the changing patterns of the susceptibility, and be able to update treatment recommendations on a regular basis...
  38. doi request reprint Evaluation of polymerase chain reaction assays for the diagnosis of Trichomonas vaginalis infection in Russia
    E Shipitsyna
    Laboratory of Microbiology, DO Ott Research Institute of Obstetrics and Gynaecology, St Petersburg, Russia
    J Eur Acad Dermatol Venereol 27:e217-23. 2013
    ..Recent years, domestically produced diagnostic PCR assays have been implemented; however, any evaluation of these PCRs has never been internationally reported...
  39. pmc Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponA
    Robert Lindberg
    Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Antimicrob Agents Chemother 51:2117-22. 2007
    ..In conclusion, an unambiguous association between penA mosaic alleles, in conjunction with genetic polymorphisms in mtrR, porB1b, and ponA, and greater reduced susceptibility to cefixime and ceftriaxone was identified...
  40. pmc Molecular epidemiology of Neisseria gonorrhoeae: sequence analysis of the porB gene confirms presence of two circulating strains
    Magnus Unemo
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    J Clin Microbiol 40:3741-9. 2002
    ..gonorrhoeae strains, as well as for confirmation or discrimination of clusters of gonorrhea cases...
  41. ncbi request reprint Genosubtyping by sequencing group A, B and C meningococci; a tool for epidemiological studies of epidemics, clusters and sporadic cases
    P Molling
    Department of Clinical Microbiology and Immunology, Orebro Medical Centre Hospital, Sweden
    APMIS 108:509-16. 2000
    ..Nucleotide sequence analysis of VRs in the porA gene proved a valuable epidemiological tool since almost all isolates could be genosubtyped, in contrast to the phenotypic methods presently used...
  42. doi request reprint Propionibacterium acnes as an etiological agent of arthroplastic and osteosynthetic infections--two cases with specific clinical presentation including formation of draining fistulae
    B Soderquist
    Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Anaerobe 16:304-6. 2010
    ..These infections are insidious with delayed presentation of symptoms and may have been overlooked because of the consideration of P. acnes as a contaminating commensal as well as the frequent use of suboptimal culture procedures...
  43. doi request reprint Evaluation of the new COBAS TaqMan CT test v2.0 and impact on the proportion of new variant Chlamydia trachomatis by the introduction of diagnostics detecting new variant C trachomatis in Orebro county, Sweden
    R Hadad
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Sex Transm Infect 85:190-3. 2009
    ....
  44. doi request reprint The Neisseria gonorrhoeae population in Sweden during 2005-phenotypes, genotypes and antibiotic resistance
    Birgitta Olsen
    Department of Clinical Medicine, Orebro University, Orebro, Sweden
    APMIS 116:181-9. 2008
    ..gonorrhoeae strains circulating in our societies, is crucial...
  45. ncbi request reprint Transformation of ciprofloxacin-resistant Neisseria gonorrhoeae gyrA, parE and porB1b genes
    Emma Lindback
    Division of Clinical Bacteriology, Department of Laboratory Medicine, Karolinska Institute, F82 Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    Int J Antimicrob Agents 28:206-11. 2006
    ..The ciprofloxacin-resistant transformants had the donor porB1b sequence, and most of them also had altered serovars. We conclude that alterations in N. gonorrhoeae PorB1b could be involved in ciprofloxacin resistance...
  46. doi request reprint Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Sweden during 2000-2007
    Emma Johansson
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    APMIS 117:900-4. 2009
    ..Wherever high reliance is placed on PIP activity for N. gonorrhoeae species confirmation, changes in the diagnostic strategies may need to be considered and/or monitoring of the PIP activity is crucial...
  47. doi request reprint Simultaneous species identification and detection of rifampicin resistance in staphylococci by sequencing of the rpoB gene
    B Hellmark
    Department of Clinical Microbiology, Orebro University Hospital, 701 85, Orebro, Sweden
    Eur J Clin Microbiol Infect Dis 28:183-90. 2009
    ..In conclusion, rpoB sequencing is a rapid, objective and accurate method of species identification and simultaneous detection of rifampicin resistance in staphylococci...
  48. pmc Total variation in the penA gene of Neisseria meningitidis: correlation between susceptibility to beta-lactam antibiotics and penA gene heterogeneity
    Sara Thulin
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 50:3317-24. 2006
    ..By studying the penA gene, genotypical determination of susceptibility in culture-negative cases can also be accomplished...
  49. pmc Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard
    Torbjörn Norén
    Department of Laboratory Medicine, Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    J Clin Microbiol 49:710-1. 2011
    ..A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings...
  50. ncbi request reprint Molecular genetic methods for diagnosis and characterisation of Chlamydia trachomatis and Neisseria gonorrhoeae: impact on epidemiological surveillance and interventions
    Hans Fredlund
    Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    APMIS 112:771-84. 2004
    ..trachomatis. This is due to the low genetic variability and hence the limited benefits for partner notification. However, genotyping of C. trachomatis may play an important role under special circumstances...
  51. ncbi request reprint Sequence constancies and variations in genes encoding three new meningococcal vaccine candidate antigens
    Susanne Jacobsson
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Vaccine 24:2161-8. 2006
    ..Based on gene prevalence and conservation, all three antigens are promising candidates for an effective meningococcal vaccine against all N. meningitidis irrespective of serogroup...
  52. ncbi request reprint Genotypic and phenotypic stability of Helicobacter pylori markers in a nine-year follow-up study of patients with noneradicated infection
    Anders Gustavsson
    Department of Medicine, Division of Gastroenterology, Orebro University Hospital, Orebro, Sweden
    Dig Dis Sci 50:375-80. 2005
    ..pylori than previously regarded. This might be true for a community with low infection and transmission rates. Complementary techniques like microarrays might, however, disclose evolutionary changes not identified here...
  53. ncbi request reprint Molecular characterisation of group A Neisseria meningitidis isolated in Sudan 1985-2001
    Susanne Jacobsson
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    APMIS 111:1060-6. 2003
    ..However, the PFGE patterns showed changes over time within the clones, where SpeI revealed somewhat more diversity than NheI...
  54. doi request reprint Antibiotic susceptibility of invasive Neisseria meningitidis isolates from 1995 to 2008 in Sweden--the meningococcal population remains susceptible
    Sara Thulin Hedberg
    National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
    Scand J Infect Dis 42:61-4. 2010
    ..In general, these remain highly susceptible to the antibiotics recommended for use. Accordingly, penicillin G remains effective for the treatment of invasive meningococcal disease and ciprofloxacin appropriate for prophylaxis...
  55. doi request reprint Characterisation of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing in a Swedish county before and after identification of the new variant
    Margaretha Jurstrand
    Clinical Research Centre, Orebro University Hospital, Orebro SE 70185, Sweden
    Sex Transm Infect 86:56-60. 2010
    ..The study also aimed to evaluate the discriminatory capacity of multilocus sequence typing (MLST) compared with ompA sequencing...
  56. pmc Antibiotic susceptibility and characteristics of Neisseria meningitidis isolates from the African meningitis belt, 2000 to 2006: phenotypic and genotypic perspectives
    Sara Thulin Hedberg
    Department of Laboratory Medicine, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, Orebro, Sweden
    Antimicrob Agents Chemother 53:1561-6. 2009
    ..Regarding beta-lactam antibiotics, rare isolates showed a reduced susceptibility to penicillins, but the expanded-spectrum cephalosporins are not affected at present...
  57. doi request reprint Emergence of the new variant of Chlamydia trachomatis in a defined area of Sweden before 2002?
    Margaretha Jurstrand
    Clinical Research Centre, Orebro University Hospital, Orebro, Sweden
    Sex Transm Infect 86:337-41. 2010
    ....
  58. pmc Combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high-throughput identification of reduced susceptibility to penicillins in Neisseria meningitidis
    Sara Thulin
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Antimicrob Agents Chemother 52:753-6. 2008
    ..All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pen(i)-specific sites and reduced susceptibility to penicillins was identified...
  59. doi request reprint Prevalence and sequence variations of the genes encoding the five antigens included in the novel 5CVMB vaccine covering group B meningococcal disease
    Susanne Jacobsson
    National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Vaccine 27:1579-84. 2009
    ..GNA2132 (54% of the amino acids conserved) appeared to be the most diversified antigen. Consequently, the theoretical coverage of the 5CVMB antigens and the feasibility to use these in a broad-range meningococcal vaccine is appealing...
  60. doi request reprint Population-based assessment of Mycoplasma genitalium in Vietnam--low prevalence among married women of reproductive age in a rural area
    B Olsen
    School of Health and Medical Sciences, Orebro University, Orebro, Sweden
    J Eur Acad Dermatol Venereol 23:533-7. 2009
    ..To analyse the prevalence of Mycoplasma genitalium infection in a population-based study among married women from a demographic surveillance site in a rural geographical area of Vietnam...
  61. doi request reprint Clostridium difficile PCR ribotype 046 is common among neonatal pigs and humans in Sweden
    T Noren
    Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
    Clin Microbiol Infect 20:O2-6. 2014
    ..Human C. difficile infection outbreaks in southern Sweden by the identical PCR ribotype 046 indicate its zoonotic potential...
  62. pmc Mutations in fusA associated with posttherapy fusidic acid resistance in Clostridium difficile
    T Noren
    Department of Infectious Diseases, Orebro University Hospital, SE 701 85 Orebro, Sweden
    Antimicrob Agents Chemother 51:1840-3. 2007
    ..Five of these mutations have previously been described in fusA of fusidic acid-resistant Staphylococcus aureus, but seven were novel fusA mutations. Fusidic acid monotherapy for CDAD seemed to rapidly select conserved resistant mutants...
  63. pmc Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone
    Makoto Ohnishi
    National Institute of Infectious Diseases, Tokyo, Japan
    Antimicrob Agents Chemother 55:3538-45. 2011
    ..Ultimately, the development of new drugs for efficacious gonorrhea treatment is necessary...
  64. ncbi request reprint The sialic acid binding SabA adhesin of Helicobacter pylori is essential for nonopsonic activation of human neutrophils
    Magnus Unemo
    Department of Clinical Microbiology, Orebro University Hospital
    J Biol Chem 280:15390-7. 2005
    ..Collectively our data suggest that the sialic acid-binding SabA adhesin is a prerequisite for the nonopsonic activation of human neutrophils and, thus, is a virulence factor important for the pathogenesis of H. pylori infection...
  65. ncbi request reprint One year of Neisseria gonorrhoeae isolates in Sweden: the prevalence study of antibiotic susceptibility shows relation to the geographic area of exposure
    Torsten Berglund
    Unit for Infectious Disease Epidemiology, Microbiology and Tumour Biology Centre, Karolinska Institute, Stockholm, Sweden
    Int J STD AIDS 13:109-14. 2002
    ..5% of the isolates from patients exposed in other places (RR=8.5, P<0.001). Ciprofloxacin cannot be recommended as the first choice of treatment if the place of exposure was in Asia...
  66. ncbi request reprint PCR of cerebrospinal fluid for diagnosis of bacterial meningitis during meningococcal epidemics; an example from Sudan
    Mohamed Issa
    Department of Microbiology and Parasitology, College of Medicine, Juba University, Sudan
    Scand J Infect Dis 35:719-23. 2003
    ..It is important to stress the use of direct and specific PCRs to avoid interference by contaminating bacteria, a great problem in samples from areas in the meningitis belt...
  67. doi request reprint Comparison of microscopy, culture and in-house PCR and NASBA assays for diagnosis of Neisseria gonorrhoeae in Russia
    Elena Shipitsyna
    D O Ott Research Institute of Obstetrics and Gynecology, St Petersburg, Russia
    APMIS 116:133-8. 2008
    ..However, in general the diagnosis of gonorrhoea in Russia is suboptimal and crucially requires validation, improvements and quality assurance...
  68. pmc Increased sporulation rate of epidemic Clostridium difficile Type 027/NAP1
    Thomas Akerlund
    Swedish Institute for Infectious Disease Control, Department of Bacteriology, SE 171 82, Solna, Sweden
    J Clin Microbiol 46:1530-3. 2008
    ..Type 027 produced 3- to 13-fold more toxin than did major Swedish types. One epidemic strain (027/NAP1a) sporulated more than did other type 027 isolates, a feature that should contribute to its survival and spread...
  69. ncbi request reprint A premature infant with fulminant meningococcal septicaemia
    Bo Selander
    Department of Paediatrics, Lund University Hospital, Lund, Sweden
    Scand J Infect Dis 35:345-8. 2003
    ..2%) were asymptomatic carriers of N. meningitidis. Serotyping and pulsed-field gel electrophoresis of the genomic DNA of the N. meningitidis isolates revealed that the infant and his father had closely related strains...
  70. pmc Slow genetic divergence of Helicobacter pylori strains during long-term colonization
    Annelie Lundin
    Department of Bacteriology, Swedish Institute for Infectious Disease Control, SE 171 82 Solna, Sweden
    Infect Immun 73:4818-22. 2005
    ..These results suggest that previous estimates of the rate of genetic change in H. pylori within an individual might be overestimates...
  71. pmc Detection of gyrA mutations associated with ciprofloxacin resistance in Neisseria gonorrhoeae by rapid and reliable pre-programmed short DNA sequencing
    Baback Gharizadeh
    Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
    Int J Antimicrob Agents 26:486-90. 2005
    ..The new method used in the present study has the potential for rapid and reliable identification of known as well as previously unknown drug resistance mutations...
  72. ncbi request reprint Pooling samples: the key to sensitive, specific and cost-effective genetic diagnosis of Chlamydia trachomatis in low-resource countries
    Elena Shipitsyna
    Laboratory of Microbiology, DO Ott Research Institute of Obstetrics and Gynecology RAMS, St Petersburg, Russia
    Acta Derm Venereol 87:140-3. 2007
    ..3% (5 samples per pool) and 44.0% (10 samples per pool). Pooling samples for PCR detection of C. trachomatis is an accurate and cost-saving approach for diagnosis and large-scale prevalence studies in St Petersburg, Russia...
  73. doi request reprint Clinical validation of a real-time polymerase chain reaction detection of Neisseria gonorrheae porA pseudogene versus culture techniques
    Stig Ove Hjelmevoll
    Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway
    Sex Transm Dis 35:517-20. 2008
    ..This study was conducted to clinically validate a previously published real-time polymerase chain reaction (PCR) method targeting the porA pseudogene in N. gonorrheae in comparison to culture techniques...
  74. pmc Pyrosequencing of the DNA gyrase gene in Neisseria species: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeae
    Emma Lindback
    Department of Clinical Bacteriology, Division of Laboratory Medicine, Karolinska University Hospital Huddinge, Stockholm, Sweden
    APMIS 114:837-41. 2006
    ..gonorrhoeae and seems unsuitable for species verification of N. gonorrhoeae. However, whether it is also possible to use this region for verification depends on the specificity of the primary screening method used...
  75. pmc A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene
    Stig Ove Hjelmevoll
    Department of Microbiology and Infection Control, University Hospital of North Norway, 9038 Tromsø, Norway
    J Mol Diagn 8:574-81. 2006
    ..In conclusion, the N. gonorrhoeae porA pseudogene real-time PCR developed in the present study is highly sensitive, specific, robust, rapid and reproducible, making it suitable for diagnosis of N. gonorrhoeae infection...
  76. ncbi request reprint Neisseria meningitidis serogroup W-135 isolated from healthy carriers and patients in Sudan after the Hajj in 2000
    Mohamed Issa
    Department of Microbiology and Parasitology, College of Medicine, Juba University, Sudan
    Scand J Infect Dis 35:230-3. 2003
    ..This strain has not been diagnosed before in Sudan. Close epidemiological surveillance is required to identify a possible new emerging meningitis epidemic...
  77. pmc Antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, Russia
    Veronica Vorobieva
    Department of Microbiology and Virology, University of Tromsø, Tromsø, Norway
    Sex Transm Infect 83:133-5. 2007
    ..To characterise comprehensively the antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, Russia, and to investigate whether the recommended treatment guidelines are updated and effective...
  78. ncbi request reprint Diagnosis of Chlamydia trachomatis in Russia--in-house PCR assays may be effective but overall optimization and quality assurance are urgently needed
    Kira Shalepo
    D O Ott Research Institute of Obstetrics and Gynecology RAMS, St Petersburg, Russia
    APMIS 114:500-7. 2006
    ..trachomatis. Nevertheless, in Russia the need for optimization and quality assurance of diagnostic methods for C. trachomatis, especially Russian DIF assays, has to be emphasized...
  79. pmc Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidis
    Muhamed Kheir Taha
    Neisseria Unit, Institut Pasteur, 28 rue du Dr Roux, Paris, France
    Antimicrob Agents Chemother 51:2784-92. 2007
    ..These data argue for the use of penA sequencing to identify isolates with reduced susceptibility to penicillin G and as a tool to improve typing of meningococcal isolates, as well as to analyze DNA exchange among Neisseria species...
  80. ncbi request reprint Home sampling and pooling of vaginal samples are effective tools for genetic screening of Chlamydia trachomatis among high school female students in Lithuania
    Vesta Kucinskiene
    Department of Skin and Venereal Diseases, Kaunas University of Medicine, Kaunas, Lithuania
    Scand J Infect Dis 40:88-93. 2008
    ..By extended personal contact and internet-based communication, increased participation rates may be attained...
  81. ncbi request reprint Laboratory diagnosis of sexually transmitted infections in estonia in 2001-2002: shortcomings with impact on diagnostic quality and surveillance
    Paul Naaber
    Department of Clinical Microbiology, United Laboratories, Tartu University Clinics, Estonia
    Sex Transm Dis 32:759-64. 2005
    ....
  82. ncbi request reprint Pooling of urine specimens allows accurate and cost-effective genetic detection of Chlamydia trachomatis in Lithuania and other low-resource countries
    Rita Butylkina
    Kaunas Public Health Centre, Kaunas, Lithuania
    Scand J Infect Dis 39:209-12. 2007
    ..The strategies would be most useful for reasonably inexpensive large-scale screening, prevalence studies and even diagnostics in Lithuania and many other low-resource countries...