Research Topics
| M UnemoSummaryAffiliation: Orebro University Hospital Country: Sweden Publications
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Publications
Emergence, spread and characteristics of Neisseria gonorrhoeae isolates with in vitro decreased susceptibility and resistance to extended-spectrum cephalosporins in SwedenDaniel Golparian
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Sex Transm Infect 86:454-60. 2010....
Neisseria gonorrhoeae strain with high-level resistance to spectinomycin due to a novel resistance mechanism (mutated ribosomal protein S5) verified in NorwayMagnus Unemo
WHO Collaborating Centre for Gonorrhoea and Other STIs, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 57:1057-61. 2013..The traditional spectinomycin resistance gene (16S rRNA) was wild type. Despite this exceedingly rare finding, spectinomycin available for treatment of ceftriaxone-resistant urogenital gonorrhea would be very valuable...- Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from Vietnam, 2011Birgitta Olsen
School of Health and Medical Sciences, Orebro University, Orebro, Sweden
BMC Infect Dis 13:40. 2013..gonorrhoeae isolated in 2011 in Hanoi, Vietnam... - Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from India, Pakistan and Bhutan in 2007-2011Sunil Sethi
WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, SE 701 85, Orebro, Sweden
BMC Infect Dis 13:35. 2013..abstract:.. - Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrheaMagnus Unemo
WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, SE 701 85 Orebro, Sweden
Future Microbiol 7:1401-22. 2012..Novel treatment strategies, antimicrobials (or other compounds) and, ideally, a vaccine must be developed... - In vitro activity of the new fluoroketolide solithromycin (CEM-101) against a large collection of clinical Neisseria gonorrhoeae isolates and international reference strains, including those with high-level antimicrobial resistance: potential treatment opDaniel Golparian
WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 56:2739-42. 2012..The activity of solithromycin was mainly superior to that of other antimicrobials (n = 10) currently or previously recommended for gonorrhea treatment. Solithromycin might be an effective treatment option for gonorrhea... - Antibiotic resistance in Neisseria gonorrhoeae: origin, evolution, and lessons learned for the futureMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
Ann N Y Acad Sci 1230:E19-28. 2011..gonorrhoeae, the linkage between resistance and biological fitness of N. gonorrhoeae, lessons learned, and future perspectives are reviewed and discussed... - High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failureMagnus Unemo
WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 56:1273-80. 2012..Nevertheless, new treatment strategies and/or drugs and, ideally, a vaccine are essential to develop for efficacious gonorrhea management... - HIV, STI prevalence and risk behaviours among women selling sex in Lahore, PakistanMohsin Saeed Khan
Mohsin Saeed Khan, Division of Global Health IHCAR, Department of Public Health Sciences, Karolinska Institutet, Stockholm, Sweden
BMC Infect Dis 11:119. 2011..The aim of our study was to estimate the prevalence of HIV and STIs, and assess knowledge and risk behaviours related to HIV/STI, among women selling sex in Lahore, Pakistan... - Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategiesM Unemo
Department of Clinical Microbiology, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, SE 701 85 Orebro, Sweden
Sex Transm Infect 83:47-51. 2007..This enzyme has previously been considered to be almost universally present in N gonorrhoeae. However, increasing numbers of N gonorrhoeae isolates lacking PIP activity have been identified... - The Swedish new variant of Chlamydia trachomatis: genome sequence, morphology, cell tropism and phenotypic characterizationMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Orebro University Hospital, Orebro, Sweden
Microbiology 156:1394-404. 2010..Therefore, the rapid transmission of nvCT in Sweden was due to the strong diagnostic selective advantage and its introduction into a high-frequency transmitting population... - Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposesMagnus Unemo
Department of Clinical Microbiology, Orebro University Hospital, Sweden
J Antimicrob Chemother 63:1142-51. 2009..Here we phenotypically and genetically characterized the 2008 WHO N. gonorrhoeae reference panel, widely used under existing WHO AMR surveillance protocols... - Laboratory diagnosis of Neisseria gonorrhoeae in St Petersburg, Russia: inventory, performance characteristics and recommended optimisationsM Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Sex Transm Infect 82:41-4. 2006.... 
Experiences with the new genetic variant of Chlamydia trachomatis in Orebro county, Sweden - proportion, characteristics and effective diagnostic solution in an emergent situationM Unemo
Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Euro Surveill 12:E5-6. 2007..LightMix 480HT seemed sensitive, specific, and enabled high throughput diagnostics. However, rare low positive samples may be false-negative. Frequent surveillance and evaluations of diagnostic methods worldwide are crucial...- Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strainMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 115:231-41. 2007..gonorrhoeae in the effective treatment of gonorrhoea... - Genetic homogeneity/heterogeneity of Propionibacterium acnes isolated from patients during cardiothoracic reoperationMagnus Unemo
Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Anaerobe 13:121-6. 2007..Existence of several clonally related P. acnes isolates derived from multiple samples from patients suffering from deep SWI after cardiothoracic surgery has not previously been shown... - Neisseria gonorrhoeae population in Arkhangelsk, Russia: phenotypic and genotypic heterogeneityM Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Clin Microbiol Infect 13:873-8. 2007..Thorough knowledge concerning the incidence of gonorrhoea, antibiotic susceptibility and other phenotypic and genotypic characteristics of the N. gonorrhoeae strains circulating in eastern Europe is crucial... - Recommended antimicrobial treatment of uncomplicated gonorrhoea in 2009 in 11 East European countries: implementation of a Neisseria gonorrhoeae antimicrobial susceptibility programme in this region is crucialM Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro SE 701 85, Sweden
Sex Transm Infect 86:442-4. 2010..Quality-assured and quality-controlled AMR surveillance data on gonococci globally are crucial for public health purposes. In East European countries, knowledge regarding gonococcal AMR and its prevalence is limited... - Pulsed-field gel electrophoresis as an epidemiologic tool for Neisseria gonorrhoeae: identification of clusters within serovarsMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria and Unit for Infectious Disease Control, Department of Clinical Microbiology and Immunology, Orebro Medical Centre Hospital, Orebro, Sweden
Sex Transm Dis 29:25-31. 2002..The increasing incidence of gonorrhea in Sweden in 1998 was due to mostly domestic cases. Among these, two core groups were identified: homosexual men with serovar IB-2 and young heterosexuals with serovar IB-3... - Comparison of serologic and genetic porB-based typing of Neisseria gonorrhoeae: consequences for future characterizationMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
J Clin Microbiol 41:4141-7. 2003..In conclusion, analysis of short highly variable regions of the porB gene could form the basis for a fast molecular epidemiological tool for the examination of emergence and transmission of N. gonorrhoeae strains within the community... - The Swedish new variant of Chlamydia trachomatisMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Curr Opin Infect Dis 24:62-9. 2011..This information provides an interesting insight into the emergence of new strains (how, where, and when), and the important lessons learned are discussed... - The porA pseudogene of Neisseria gonorrhoeae--low level of genetic polymorphism and a few, mainly identical, inactivating mutationsMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, Orebro, Sweden
APMIS 113:410-9. 2005..The highly conserved N. gonorrhoeae porA pseudogene may reflect an evolutionary neutral molecular clock and may be a suitable genetic target for diagnosis of N. gonorrhoeae... - Gonorrhoea surveillance, laboratory diagnosis and antimicrobial susceptibility testing of Neisseria gonorrhoeae in 11 countries of the eastern part of the WHO European regionMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Orebro University Hospital, Sweden
APMIS 119:643-9. 2011..The information provided herein will be useful in this respect... - Molecular typing of Neisseria gonorrhoeae isolates by pyrosequencing of highly polymorphic segments of the porB geneMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
J Clin Microbiol 42:2926-34. 2004..In conclusion, the fast and high-throughput pyrosequencing technology can be used for molecular epidemiological characterization of N. gonorrhoeae... - In vitro antimicrobial synergy testing of coagulase-negative staphylococci isolated from prosthetic joint infections using Etest and with a focus on rifampicin and linezolidB Hellmark
Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, 701 85 Orebro, Sweden
Eur J Clin Microbiol Infect Dis 29:591-5. 2010..In conclusion, Etest is an objective and easily performed in vitro method for antimicrobial synergy testing. However, each isolate requires testing for the specific combination considered for treatment... - In vitro susceptibility to 17 antimicrobials of clinical Clostridium difficile isolates collected in 1993-2007 in SwedenT Noren
Department of Infectious Diseases, Orebro University Hospital, SE 701 85 Orebro, Sweden
Clin Microbiol Infect 16:1104-10. 2010..difficile isolates showed variations of multiresistance to be due to a specific PCR ribotype 012, emphasizing the importance of genotyping when evaluating emerging resistance over time... - Antibiotic susceptibility among Staphylococcus epidermidis isolated from prosthetic joint infections with special focus on rifampicin and variability of the rpoB geneB Hellmark
Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Clin Microbiol Infect 15:238-44. 2009..Detection of mecA is necessary before initiating treatment of infections due to S. epidermidis when it displays intermediate susceptibility to cefoxitin... - Molecular epidemiology of Neisseria gonorrhoeae- identification of the first presumed Swedish transmission chain of an azithromycin-resistant strainDavid Lundbäck
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Sweden
APMIS 114:67-71. 2006..gonorrhoeae in order to identify emergence of new resistance, monitor the changing patterns of the susceptibility, and be able to update treatment recommendations on a regular basis... - Molecular epidemiology of Neisseria gonorrhoeae: sequence analysis of the porB gene confirms presence of two circulating strainsMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
J Clin Microbiol 40:3741-9. 2002..gonorrhoeae strains, as well as for confirmation or discrimination of clusters of gonorrhea cases... - Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponARobert Lindberg
Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Antimicrob Agents Chemother 51:2117-22. 2007..In conclusion, an unambiguous association between penA mosaic alleles, in conjunction with genetic polymorphisms in mtrR, porB1b, and ponA, and greater reduced susceptibility to cefixime and ceftriaxone was identified... - Propionibacterium acnes as an etiological agent of arthroplastic and osteosynthetic infections--two cases with specific clinical presentation including formation of draining fistulaeB Soderquist
Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Anaerobe 16:304-6. 2010..These infections are insidious with delayed presentation of symptoms and may have been overlooked because of the consideration of P. acnes as a contaminating commensal as well as the frequent use of suboptimal culture procedures... - Evaluation of the new COBAS TaqMan CT test v2.0 and impact on the proportion of new variant Chlamydia trachomatis by the introduction of diagnostics detecting new variant C trachomatis in Orebro county, SwedenR Hadad
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Sex Transm Infect 85:190-3. 2009.... - Genosubtyping by sequencing group A, B and C meningococci; a tool for epidemiological studies of epidemics, clusters and sporadic casesP Molling
Department of Clinical Microbiology and Immunology, Orebro Medical Centre Hospital, Sweden
APMIS 108:509-16. 2000..Nucleotide sequence analysis of VRs in the porA gene proved a valuable epidemiological tool since almost all isolates could be genosubtyped, in contrast to the phenotypic methods presently used... - The Neisseria gonorrhoeae population in Sweden during 2005-phenotypes, genotypes and antibiotic resistanceBirgitta Olsen
Department of Clinical Medicine, Orebro University, Orebro, Sweden
APMIS 116:181-9. 2008..gonorrhoeae strains circulating in our societies, is crucial... - Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Sweden during 2000-2007Emma Johansson
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 117:900-4. 2009..Wherever high reliance is placed on PIP activity for N. gonorrhoeae species confirmation, changes in the diagnostic strategies may need to be considered and/or monitoring of the PIP activity is crucial... - Transformation of ciprofloxacin-resistant Neisseria gonorrhoeae gyrA, parE and porB1b genesEmma Lindback
Division of Clinical Bacteriology, Department of Laboratory Medicine, Karolinska Institute, F82 Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
Int J Antimicrob Agents 28:206-11. 2006..The ciprofloxacin-resistant transformants had the donor porB1b sequence, and most of them also had altered serovars. We conclude that alterations in N. gonorrhoeae PorB1b could be involved in ciprofloxacin resistance... - Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standardTorbjörn Norén
Department of Laboratory Medicine, Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
J Clin Microbiol 49:710-1. 2011..A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings... - Characterisation of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing in a Swedish county before and after identification of the new variantMargaretha Jurstrand
Clinical Research Centre, Orebro University Hospital, Orebro SE 70185, Sweden
Sex Transm Infect 86:56-60. 2010..The study also aimed to evaluate the discriminatory capacity of multilocus sequence typing (MLST) compared with ompA sequencing... - Molecular genetic methods for diagnosis and characterisation of Chlamydia trachomatis and Neisseria gonorrhoeae: impact on epidemiological surveillance and interventionsHans Fredlund
Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 112:771-84. 2004..trachomatis. This is due to the low genetic variability and hence the limited benefits for partner notification. However, genotyping of C. trachomatis may play an important role under special circumstances... - Antibiotic susceptibility of invasive Neisseria meningitidis isolates from 1995 to 2008 in Sweden--the meningococcal population remains susceptibleSara Thulin Hedberg
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Scand J Infect Dis 42:61-4. 2010..In general, these remain highly susceptible to the antibiotics recommended for use. Accordingly, penicillin G remains effective for the treatment of invasive meningococcal disease and ciprofloxacin appropriate for prophylaxis... - Prevalence and sequence variations of the genes encoding the five antigens included in the novel 5CVMB vaccine covering group B meningococcal diseaseSusanne Jacobsson
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Vaccine 27:1579-84. 2009..GNA2132 (54% of the amino acids conserved) appeared to be the most diversified antigen. Consequently, the theoretical coverage of the 5CVMB antigens and the feasibility to use these in a broad-range meningococcal vaccine is appealing... - Molecular characterisation of group A Neisseria meningitidis isolated in Sudan 1985-2001Susanne Jacobsson
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 111:1060-6. 2003..However, the PFGE patterns showed changes over time within the clones, where SpeI revealed somewhat more diversity than NheI... - Antibiotic susceptibility and characteristics of Neisseria meningitidis isolates from the African meningitis belt, 2000 to 2006: phenotypic and genotypic perspectivesSara Thulin Hedberg
Department of Laboratory Medicine, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 53:1561-6. 2009..Regarding beta-lactam antibiotics, rare isolates showed a reduced susceptibility to penicillins, but the expanded-spectrum cephalosporins are not affected at present... - Genotypic and phenotypic stability of Helicobacter pylori markers in a nine-year follow-up study of patients with noneradicated infectionAnders Gustavsson
Department of Medicine, Division of Gastroenterology, Orebro University Hospital, Orebro, Sweden
Dig Dis Sci 50:375-80. 2005..pylori than previously regarded. This might be true for a community with low infection and transmission rates. Complementary techniques like microarrays might, however, disclose evolutionary changes not identified here... - Sequence constancies and variations in genes encoding three new meningococcal vaccine candidate antigensSusanne Jacobsson
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Vaccine 24:2161-8. 2006..Based on gene prevalence and conservation, all three antigens are promising candidates for an effective meningococcal vaccine against all N. meningitidis irrespective of serogroup... - Combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high-throughput identification of reduced susceptibility to penicillins in Neisseria meningitidisSara Thulin
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Antimicrob Agents Chemother 52:753-6. 2008..All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pen(i)-specific sites and reduced susceptibility to penicillins was identified... - Emergence of the new variant of Chlamydia trachomatis in a defined area of Sweden before 2002?Margaretha Jurstrand
Clinical Research Centre, Orebro University Hospital, Orebro, Sweden
Sex Transm Infect 86:337-41. 2010.... - Total variation in the penA gene of Neisseria meningitidis: correlation between susceptibility to beta-lactam antibiotics and penA gene heterogeneitySara Thulin
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 50:3317-24. 2006..By studying the penA gene, genotypical determination of susceptibility in culture-negative cases can also be accomplished... - Staphylococcal cassette chromosome mec (SCCmec) and arginine catabolic mobile element (ACME) in Staphylococcus epidermidis isolated from prosthetic joint infectionsB Hellmark
Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, 701 85, Orebro, Sweden
Eur J Clin Microbiol Infect Dis 32:691-7. 2013..ACME was more common among the commensal isolates and may represent a survival benefit for S. epidermidis colonising healthy individuals in the community... - Population-based assessment of Mycoplasma genitalium in Vietnam--low prevalence among married women of reproductive age in a rural areaB Olsen
School of Health and Medical Sciences, Orebro University, Orebro, Sweden
J Eur Acad Dermatol Venereol 23:533-7. 2009..To analyse the prevalence of Mycoplasma genitalium infection in a population-based study among married women from a demographic surveillance site in a rural geographical area of Vietnam... - Simultaneous species identification and detection of rifampicin resistance in staphylococci by sequencing of the rpoB geneB Hellmark
Department of Clinical Microbiology, Orebro University Hospital, 701 85, Orebro, Sweden
Eur J Clin Microbiol Infect Dis 28:183-90. 2009..In conclusion, rpoB sequencing is a rapid, objective and accurate method of species identification and simultaneous detection of rifampicin resistance in staphylococci... - Mutations in fusA associated with posttherapy fusidic acid resistance in Clostridium difficileT Noren
Department of Infectious Diseases, Orebro University Hospital, SE 701 85 Orebro, Sweden
Antimicrob Agents Chemother 51:1840-3. 2007..Five of these mutations have previously been described in fusA of fusidic acid-resistant Staphylococcus aureus, but seven were novel fusA mutations. Fusidic acid monotherapy for CDAD seemed to rapidly select conserved resistant mutants... - Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxoneMakoto Ohnishi
National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 55:3538-45. 2011..Ultimately, the development of new drugs for efficacious gonorrhea treatment is necessary... - The sialic acid binding SabA adhesin of Helicobacter pylori is essential for nonopsonic activation of human neutrophilsMagnus Unemo
Department of Clinical Microbiology, Orebro University Hospital
J Biol Chem 280:15390-7. 2005..Collectively our data suggest that the sialic acid-binding SabA adhesin is a prerequisite for the nonopsonic activation of human neutrophils and, thus, is a virulence factor important for the pathogenesis of H. pylori infection... - PCR of cerebrospinal fluid for diagnosis of bacterial meningitis during meningococcal epidemics; an example from SudanMohamed Issa
Department of Microbiology and Parasitology, College of Medicine, Juba University, Sudan
Scand J Infect Dis 35:719-23. 2003..It is important to stress the use of direct and specific PCRs to avoid interference by contaminating bacteria, a great problem in samples from areas in the meningitis belt... - Neisseria meningitidis serogroup W-135 isolated from healthy carriers and patients in Sudan after the Hajj in 2000Mohamed Issa
Department of Microbiology and Parasitology, College of Medicine, Juba University, Sudan
Scand J Infect Dis 35:230-3. 2003..This strain has not been diagnosed before in Sudan. Close epidemiological surveillance is required to identify a possible new emerging meningitis epidemic... - Slow genetic divergence of Helicobacter pylori strains during long-term colonizationAnnelie Lundin
Department of Bacteriology, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden
Infect Immun 73:4818-22. 2005..These results suggest that previous estimates of the rate of genetic change in H. pylori within an individual might be overestimates... - One year of Neisseria gonorrhoeae isolates in Sweden: the prevalence study of antibiotic susceptibility shows relation to the geographic area of exposureTorsten Berglund
Unit for Infectious Disease Epidemiology, Microbiology and Tumour Biology Centre, Karolinska Institute, Stockholm, Sweden
Int J STD AIDS 13:109-14. 2002..5% of the isolates from patients exposed in other places (RR=8.5, P<0.001). Ciprofloxacin cannot be recommended as the first choice of treatment if the place of exposure was in Asia... - A premature infant with fulminant meningococcal septicaemiaBo Selander
Department of Paediatrics, Lund University Hospital, Lund, Sweden
Scand J Infect Dis 35:345-8. 2003..2%) were asymptomatic carriers of N. meningitidis. Serotyping and pulsed-field gel electrophoresis of the genomic DNA of the N. meningitidis isolates revealed that the infant and his father had closely related strains... - Detection of gyrA mutations associated with ciprofloxacin resistance in Neisseria gonorrhoeae by rapid and reliable pre-programmed short DNA sequencingBaback Gharizadeh
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
Int J Antimicrob Agents 26:486-90. 2005..The new method used in the present study has the potential for rapid and reliable identification of known as well as previously unknown drug resistance mutations... - Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidisMuhamed Kheir Taha
Neisseria Unit, Institut Pasteur, 28 rue du Dr Roux, Paris, France
Antimicrob Agents Chemother 51:2784-92. 2007..These data argue for the use of penA sequencing to identify isolates with reduced susceptibility to penicillin G and as a tool to improve typing of meningococcal isolates, as well as to analyze DNA exchange among Neisseria species... - Increased sporulation rate of epidemic Clostridium difficile Type 027/NAP1Thomas Akerlund
Swedish Institute for Infectious Disease Control, Department of Bacteriology, SE 171 82, Solna, Sweden
J Clin Microbiol 46:1530-3. 2008..Type 027 produced 3- to 13-fold more toxin than did major Swedish types. One epidemic strain (027/NAP1a) sporulated more than did other type 027 isolates, a feature that should contribute to its survival and spread... - Comparison of microscopy, culture and in-house PCR and NASBA assays for diagnosis of Neisseria gonorrhoeae in RussiaElena Shipitsyna
D O Ott Research Institute of Obstetrics and Gynecology, St Petersburg, Russia
APMIS 116:133-8. 2008..However, in general the diagnosis of gonorrhoea in Russia is suboptimal and crucially requires validation, improvements and quality assurance... - Clinical validation of a real-time polymerase chain reaction detection of Neisseria gonorrheae porA pseudogene versus culture techniquesStig Ove Hjelmevoll
Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway
Sex Transm Dis 35:517-20. 2008..This study was conducted to clinically validate a previously published real-time polymerase chain reaction (PCR) method targeting the porA pseudogene in N. gonorrheae in comparison to culture techniques... - Pooling samples: the key to sensitive, specific and cost-effective genetic diagnosis of Chlamydia trachomatis in low-resource countriesElena Shipitsyna
Laboratory of Microbiology, DO Ott Research Institute of Obstetrics and Gynecology RAMS, St Petersburg, Russia
Acta Derm Venereol 87:140-3. 2007..3% (5 samples per pool) and 44.0% (10 samples per pool). Pooling samples for PCR detection of C. trachomatis is an accurate and cost-saving approach for diagnosis and large-scale prevalence studies in St Petersburg, Russia... - Pyrosequencing of the DNA gyrase gene in Neisseria species: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeaeEmma Lindback
Department of Clinical Bacteriology, Division of Laboratory Medicine, Karolinska University Hospital Huddinge, Stockholm, Sweden
APMIS 114:837-41. 2006..gonorrhoeae and seems unsuitable for species verification of N. gonorrhoeae. However, whether it is also possible to use this region for verification depends on the specificity of the primary screening method used... - A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogeneStig Ove Hjelmevoll
Department of Microbiology and Infection Control, University Hospital of North Norway, 9038 Tromsø, Norway
J Mol Diagn 8:574-81. 2006..In conclusion, the N. gonorrhoeae porA pseudogene real-time PCR developed in the present study is highly sensitive, specific, robust, rapid and reproducible, making it suitable for diagnosis of N. gonorrhoeae infection... - Antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, RussiaVeronica Vorobieva
Department of Microbiology and Virology, University of Tromsø, Tromsø, Norway
Sex Transm Infect 83:133-5. 2007..To characterise comprehensively the antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, Russia, and to investigate whether the recommended treatment guidelines are updated and effective... - Diagnosis of Chlamydia trachomatis in Russia--in-house PCR assays may be effective but overall optimization and quality assurance are urgently neededKira Shalepo
D.O. Ott Research Institute of Obstetrics and Gynecology RAMS, St. Petersburg, Russia
APMIS 114:500-7. 2006..trachomatis. Nevertheless, in Russia the need for optimization and quality assurance of diagnostic methods for C. trachomatis, especially Russian DIF assays, has to be emphasized... - Laboratory diagnosis of sexually transmitted infections in estonia in 2001-2002: shortcomings with impact on diagnostic quality and surveillancePaul Naaber
Department of Clinical Microbiology, United Laboratories, Tartu University Clinics, Estonia
Sex Transm Dis 32:759-64. 2005.... - Home sampling and pooling of vaginal samples are effective tools for genetic screening of Chlamydia trachomatis among high school female students in LithuaniaVesta Kucinskiene
Department of Skin and Venereal Diseases, Kaunas University of Medicine, Kaunas, Lithuania
Scand J Infect Dis 40:88-93. 2008..By extended personal contact and internet-based communication, increased participation rates may be attained... - Pooling of urine specimens allows accurate and cost-effective genetic detection of Chlamydia trachomatis in Lithuania and other low-resource countriesRita Butylkina
Kaunas Public Health Centre, Kaunas, Lithuania
Scand J Infect Dis 39:209-12. 2007..The strategies would be most useful for reasonably inexpensive large-scale screening, prevalence studies and even diagnostics in Lithuania and many other low-resource countries...