Research Topics
Genomes and Genes | Jure PiskurSummaryAffiliation: Lund University Country: Sweden Publications
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Publications
Yeast genome duplication was followed by asynchronous differentiation of duplicated genesRikke B Langkjaer
BioCentrum-DTU, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark
Nature 421:848-52. 2003..Further evolution of each duplicated gene pair-such as specialization or differentiation of the two copies, or deletion of a single copy--has taken place independently throughout the evolution of these species...
A second pathway to degrade pyrimidine nucleic acid precursors in eukaryotesGorm Andersen
Department of Cell and Organism Biology, Lund University, 223 62 Lund, Sweden
J Mol Biol 380:656-66. 2008..The genes of the URC pathway are not homologous to any of the eukaryotic or prokaryotic genes involved in pyrimidine degradation described to date...- The genome of wine yeast Dekkera bruxellensis provides a tool to explore its food-related propertiesJure Piskur
Wine Research Centre, University of Nova Gorica, Nova Gorica, Slovenia
Int J Food Microbiol 157:202-9. 2012..cerevisiae lineage. However, D. bruxellensis has several independently duplicated ADH and ADH-like genes, which are likely responsible for metabolism of alcohols, including ethanol, and also a range of aromatic compounds... - How did Saccharomyces evolve to become a good brewer?Jure Piskur
Cell and Organism Biology, Lund University, Sölvegatan 35, 22362 Lund, Sweden
Trends Genet 22:183-6. 2006..Two research groups have shed light on the origin of the genes encoding alcohol dehydrogenase and the process of ethanol accumulation in Saccharomyces cerevisiae... - Comparative genomics reveals novel biochemical pathwaysJure Piskur
Department of Cell and Organism Biology, Lund University, Sölvegatan 35, 22362 Lund, Sweden
Trends Genet 23:369-72. 2007.... 
Parallel evolution of the make-accumulate-consume strategy in Saccharomyces and Dekkera yeastsElzbieta Rozpedowska
Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden
Nat Commun 2:302. 2011..bruxellensis this promoter rewiring contributes to the observed Crabtree effect...- Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thalianaAnders R Clausen
Department of Cell and Organism Biology, Lund University, Lund, Sweden
FEBS J 279:3889-97. 2012..The results obtained in the present study suggest a crucial role for the salvage of thymidine during early plant development... - Candida albicans--a pre-whole genome duplication yeast--is predominantly aerobic and a poor ethanol producerElzbieta Rozpedowska
Department of Biology, Lund University, Lund, Sweden
FEMS Yeast Res 11:285-91. 2011..Apparently, C. albicans and several other pre-WGD yeasts still exhibit the original traits of the yeast progenitor: poor accumulation of ethanol under aerobic conditions and strong dependence on the presence of oxygen... - Saccharomyces kluyveri as a model organism to study pyrimidine degradationHalfdan Beck
Department of Cell and Organism Biology, Lund University, Lund, Sweden
FEMS Yeast Res 8:1209-13. 2008..kluyveri and how this increases our understanding of human disease. Additionally, we discuss the possibilities and limitations of this nonconventional yeast as a laboratory organism... - Thymidine kinase 1 regulatory fine-tuning through tetramer formationZeeshan Mutahir
Department of Biology, Lund University, Lund, Sweden
FEBS J 280:1531-41. 2013..The efficient switching mechanism was probably first established in warm-blooded animals when they separated from the rest of the vertebrates... - Complex nature of the genome in a wine spoilage yeast, Dekkera bruxellensisLinda Hellborg
Department of Cell and Organism Biology, Lund University, Sölvegatan 35, Lund SE 223 62, Sweden
Eukaryot Cell 8:1739-49. 2009.... - Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosineTinkara Tinta
Department of Biology, Lund University, Lund, Sweden
FEMS Microbiol Lett 331:120-7. 2012..When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies... - Elucidation of the sex-pheromone biosynthesis producing 5,7-dodecadienes in Dendrolimus punctatus (Lepidoptera: Lasiocampidae) reveals Delta 11- and Delta 9-desaturases with unusual catalytic propertiesMarjorie A Liénard
Chemical Ecology and Ecotoxicology, Department of Biology, Lund University, Lund, Sweden
Insect Biochem Mol Biol 40:440-52. 2010..The combined action of three desaturases in generating a dienoic sex-pheromone component emphasizes the diversity and complexity of chemical reactions that can be catalysed by pheromone biosynthetic fatty-acyl-CoA desaturases in moths... - Formation of new chromosomes as a virulence mechanism in yeast Candida glabrataSilvia Polakova
Department of Cell and Organism Biology, Lund University, SE 22362 Lund, Sweden
Proc Natl Acad Sci U S A 106:2688-93. 2009..We show that the observed genome plasticity is connected with antifungal drug resistance and it is likely an advantage in the human body, where environmental conditions fluctuate a lot... - A recruited protease is involved in catabolism of pyrimidinesBirgit Andersen
Department of Cell and Organism Biology, Lund University, SE 22362 Lund, Sweden
J Mol Biol 379:243-50. 2008.... - Complex evolution of the DAL5 transporter familyLinda Hellborg
Cell and Organism Biology, Lund University, Lund, Sweden
BMC Genomics 9:164. 2008..We studied the complex evolution of this group in species from the kingdom of fungi particularly the Ascomycetes... - Deoxyribonucleoside kinases: two enzyme families catalyze the same reactionMichael P B Sandrini
Cell and Organism Biology, Lund University, Sölvegatan 35, SE 22732 Lund, Sweden
Trends Biochem Sci 30:225-8. 2005..Recently, the crystal structure of human TK1 has been solved and has revealed that enzymes with fundamentally different origins and folds catalyze similar, crucial cellular reactions... - Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassaysMikael Evander
Department of Electrical Measurements, Lund University, Lund, Sweden
Anal Chem 79:2984-91. 2007..The demonstrated acoustic trapping of cells and particles enables cell- or particle-based bioassays to be performed in a continuous flow format... - Transfer of genetic material between pathogenic and food-borne yeastsMarek Mentel
Department of Cell and Organism Biology, Lund University, , SE-223 62 Lund, Sweden
Appl Environ Microbiol 72:5122-5. 2006..However, high-frequency transfer of plasmid DNA was observed when pathogenic and food-borne yeasts were grown together. This property could play a crucial role in the spread of virulence and drug resistance factors among yeasts... - Fermentative lifestyle in yeasts belonging to the Saccharomyces complexAnnamaria Merico
Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milan, Italy
FEBS J 274:976-89. 2007..However, these traits have been combined and developed to perfection only in the lineage that underwent the whole-genome duplication and led to the modern Saccharomyces cerevisiae yeast... - Dihydropyrimidine amidohydrolases and dihydroorotases share the same origin and several enzymatic propertiesZoran Gojkovic
Eukaryote Molecular Biology, BioCentrum DTU, Technical University of Denmark, Building 301, DK 2800 Lyngby, Denmark
Nucleic Acids Res 31:1683-92. 2003..Therefore, only a few modifications might have been necessary during evolution to convert the unspecialized enzyme into anabolic and catabolic ones... - Steady-state and transient-state analyses of aerobic fermentation in Saccharomyces kluyveriKasper Møller
Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
FEMS Yeast Res 2:233-44. 2002..kluyveri led to ethanol formation after a delay of 20-50 min (depending on culture conditions prior to the pulse), which is in contrast to S. cerevisiae that ferments immediately after glucose addition... - Animal deoxyribonucleoside kinases: 'forward' and 'retrograde' evolution of their substrate specificityJure Piskur
BioCentrum DTU, Eukaryote Molecular Biology Group, Building 301, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
FEBS Lett 560:3-6. 2004..Thereafter, insects lost all but one kinase, dNK (EC 2.7.1.145), which subsequently, through remodelling of a limited number of amino acid residues, gained a broad substrate specificity... - Yeast genome sequencing: the power of comparative genomicsJure Piskur
BioCentrum DTU, Building 301, Technical University of Denmark, DK 2800 Kgl Lyngby, Denmark
Mol Microbiol 53:381-9. 2004..In addition, the accumulating sequence data provide the background to use more yeast species in model studies, to combat pathogens and for efficient manipulation of industrial strains... - Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygenMarek Mentel
Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynska dolina CH-I, 842 15 Bratislava, Slovakia
Mol Genet Genomics 273:84-91. 2005..lipolytica and the facultative anaerobe S. cerevisiae, the three paralogs are differentially regulated in the absence of oxygen. Apparently, Y. lipolytica can sense hypoxia and down-regulate target genes in response... - Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific mannerMichael P B Sandrini
BioCentrum DTU, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
J Antimicrob Chemother 60:510-20. 2007..To investigate the bactericidal activity of antiviral and anticancer nucleoside analogues against a variety of pathogenic bacteria and characterize the activating enzymes, deoxyribonucleoside kinases (dNKs)... - A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeastGorm Andersen
BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
FEBS J 274:1804-17. 2007..It is likely that upon duplication approximately 200 million years ago, a specialized Uga1p evolved into a 'novel' transaminase enzyme with broader substrate specificity... - The crystal structures of dihydropyrimidinases reaffirm the close relationship between cyclic amidohydrolases and explain their substrate specificityBernhard Lohkamp
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17177 Stockholm
J Biol Chem 281:13762-76. 2006.... - Deoxyribonucleoside kinases belonging to the thymidine kinase 2 (TK2)-like group vary significantly in substrate specificity, kinetics and feed-back regulationWolfgang Knecht
Section of Molecular Microbiology, BioCentrum DTU, Building 301, Technical University of Denmark, Lyngby, DK 2800, Denmark
J Mol Biol 315:529-40. 2002..The broad substrate specificity and different feedback regulation of the multisubstrate insect deoxyribonucleoside kinases may indicate that these enzymes have a different functional role than the other members of the TK2-like group... - Inheritance and organisation of the mitochondrial genome differ between two Saccharomyces yeastsRandi F Petersen
BioCentrum DTU, Technical University of Denmark, Building 301, DK 2800 Kgl Lyngby, Denmark
J Mol Biol 318:627-36. 2002..cerevisiae. The structure of one suppressive S. castellii mutant, CA38, was also determined. Apparently, a short direct intergenic repeat was involved in the generation of this petite mtDNA molecule... - Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensisMegan Woolfit
Department of Genetics, Smurfit Institute, Trinity College Dublin, Dublin 2, Ireland
Eukaryot Cell 6:721-33. 2007..These data provide a resource for further analyses of the population genetics and evolution of D. bruxellensis and of the genetic bases of its physiological capabilities... - Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicansElke Zameitat
Institute for Physiological Chemistry, Philipps University, Marburg, Germany
FEBS J 273:3183-91. 2006..albicans DHODH activity. This study provides a background for the development of antipyrimidines with high efficacy for decreasing in situ pyrimidine nucleotide pools in C. albicans... - High-rate evolution of Saccharomyces sensu lato chromosomesMario Spirek
Eukaryote Molecular Biology Group, BioCentrum DTU, Technical University of Denmark, Building 301, 2800, Lyngby, Denmark
FEMS Yeast Res 3:363-73. 2003..Apparently, yeast chromosomes have been frequently reshaped and therefore also the position of genes has been dynamic during the evolutionary history of yeasts... - Reconstitution of an efficient thymidine salvage pathway in Saccharomyces cerevisiaeLaurence Vernis
Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, EN6 3LD, UK
Nucleic Acids Res 31:e120. 2003..We show that this strain also efficiently incorporates the thymidine analogue, BrdU, into DNA and can be used for pulse-chase labelling... - Further development of the cassette-based pYC plasmid system by incorporation of the dominant hph, nat and AUR1-C gene markers and the lacZ reporter systemJørgen Hansen
Carlsberg Research Laboratory, Gamle Carlsberg Vej 10, 2500, Copenhagen Valby, Denmark
FEMS Yeast Res 4:323-7. 2003..Furthermore, the lacZ system for analyzing gene expression was included in the system... - Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complexVeronika Fekete
Comenius University, Faculty of Natural Sciences, Department of Biochemistry, Mlynska dolina, Bratislava, Slovakia
FEMS Yeast Res 7:1237-47. 2007..However, petite-positive traits could also be observed among the prewhole-genome duplication species... - Crystal structures of yeast beta-alanine synthase complexes reveal the mode of substrate binding and large scale domain closure movementsStina Lundgren
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177 Stockholm, Sweden
J Biol Chem 282:36037-47. 2007..Substrate binding is achieved via a salt bridge, a number of hydrogen bonds, and coordination to one of the zinc ions of the di-metal center... - Aerobic glucose metabolism of Saccharomyces kluyveri: growth, metabolite production, and quantification of metabolic fluxesKasper Møller
Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
Biotechnol Bioeng 77:186-93. 2002..cerevisiae at the same growth conditions, where the tricarboxylic acid cycle operates as two branches. This indicates that the respiratory system was not significantly repressed in S. kluyveri during batch cultivation on glucose...