Marie F Gorwa-Grauslund

Summary

Affiliation: Lund University
Country: Sweden

Publications

  1. pmc Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant
    Violeta Sànchez i Nogué
    Division of Applied Microbiology, Lund University, P, O, Box 124, SE 221 00, Lund, Sweden
    AMB Express 2:68. 2012
  2. pmc Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library
    Nadia Skorupa Parachin
    Department of Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund University, P, O, Box 124, SE 221 00 Lund, Sweden
    Biotechnol Biofuels 4:9. 2011
  3. pmc Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering
    Rosa Garcia Sanchez
    Department of Applied Microbiology, Lund University, PO Box 124, SE 22100 Lund, Sweden
    Biotechnol Biofuels 3:13. 2010
  4. pmc Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
    Oskar Bengtsson
    Department of Applied Microbiology, Lund University, SE 221 00 Lund, Sweden
    Biotechnol Biofuels 2:9. 2009
  5. pmc Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae
    Rosa Garcia Sanchez
    Department of Applied Microbiology, Lund University, P, O, Box 124, SE 22100 Lund, Sweden
    Biotechnol Biofuels 3:19. 2010
  6. ncbi request reprint A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance
    Anneli Petersson
    Department of Chemical Engineering, Lund University, PO Box 124, S 221 00 Lund, Sweden
    Yeast 23:455-64. 2006
  7. doi request reprint NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae
    João R M Almeida
    Department of Applied Microbiology, Lund University, Lund, Sweden
    Appl Microbiol Biotechnol 78:939-45. 2008
  8. ncbi request reprint Efficient anaerobic whole cell stereoselective bioreduction with recombinant Saccharomyces cerevisiae
    Michael Katz
    Department of Applied Microbiology, Lund University, P O Box 124, 221 00 Lund, Sweden
    Biotechnol Bioeng 84:573-82. 2003
  9. ncbi request reprint The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains
    Marie Jeppsson
    Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden
    Yeast 20:1263-72. 2003
  10. doi request reprint Stress-related challenges in pentose fermentation to ethanol by the yeast Saccharomyces cerevisiae
    João R M Almeida
    Applied Microbiology, Lund University, Lund, Sweden EMBRAPA Agroenergy, PqEB, Brasilia, 70770 901 DF, Brazil
    Biotechnol J 6:286-99. 2011

Collaborators

  • Kaisa Karhumaa
  • Tobias Modig
  • Bjorn Johansson
  • Magnus Bertilsson
  • Natalie Kostesha
  • David Runquist
  • Peter Radstrom
  • Uwe Sauer
  • Andreas Rudolf
  • Karin Ohgren
  • Romain Fromanger
  • Bärbel Hahn-Hägerdal
  • João R M Almeida
  • Oskar Bengtsson
  • Marie Jeppsson
  • Nadia Skorupa Parachin
  • Gunnar Liden
  • Rosa Garcia Sanchez
  • Violeta Sànchez i Nogué
  • Ted Johanson
  • Maurizio Bettiga
  • Magnus Carlquist
  • Michael Katz
  • Basti Bergdahl
  • Torbjorn Frejd
  • Ed W J Van Niel
  • Nádia S Parachin
  • Nádia Skorupa Parachin
  • César Fonseca
  • Christer F Spégel
  • Annika Friberg
  • Anneli Petersson
  • Anneli Nilsson
  • Márk Gárdonyi
  • Tarinee Boonyawan
  • Celina Borgström
  • Venkatachalam Narayanan
  • Anders G Sandström
  • Börje Norling
  • João Rm Almeida
  • Violeta Sànchez Nogué
  • Christer U Larsson
  • Jenny Schelin
  • Marie F Gorwa Grauslund
  • Steven Gorsich
  • Marco Sonderegger
  • Boaz Laadan
  • Cecilia Olsson
  • Anja Roder
  • Jenny Emneus
  • Tautgirdas Ruzgas
  • Isabel Spencer-Martins
  • Milena Koudelka-Hep
  • Arto R Heiskanen
  • Ted H Johanson
  • Johan Franzén
  • Hung Lee
  • Katja Franke
  • Karin Träff
  • Peter Ruhdal Jensen

Detail Information

Publications41

  1. pmc Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant
    Violeta Sànchez i Nogué
    Division of Applied Microbiology, Lund University, P, O, Box 124, SE 221 00, Lund, Sweden
    AMB Express 2:68. 2012
    ....
  2. pmc Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library
    Nadia Skorupa Parachin
    Department of Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund University, P, O, Box 124, SE 221 00 Lund, Sweden
    Biotechnol Biofuels 4:9. 2011
    ..abstract:..
  3. pmc Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering
    Rosa Garcia Sanchez
    Department of Applied Microbiology, Lund University, PO Box 124, SE 22100 Lund, Sweden
    Biotechnol Biofuels 3:13. 2010
    ..Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization. Notably, the rate and yield of xylose and arabinose co-fermentation to ethanol must be enhanced...
  4. pmc Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
    Oskar Bengtsson
    Department of Applied Microbiology, Lund University, SE 221 00 Lund, Sweden
    Biotechnol Biofuels 2:9. 2009
    ..cerevisiae strain was created that provides a unique combination of high xylose consumption rate, high ethanol yield and low xylitol yield during ethanolic xylose fermentation...
  5. pmc Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae
    Rosa Garcia Sanchez
    Department of Applied Microbiology, Lund University, P, O, Box 124, SE 22100 Lund, Sweden
    Biotechnol Biofuels 3:19. 2010
    ..cerevisiae strains engineered with the xylose reductase (XR)-xylitol dehydrogenase (XDH) pathway...
  6. ncbi request reprint A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance
    Anneli Petersson
    Department of Chemical Engineering, Lund University, PO Box 124, S 221 00 Lund, Sweden
    Yeast 23:455-64. 2006
    ..Yeast strains overexpressing ADH6 also had a substantially higher in vivo conversion rate of HMF in both aerobic and anaerobic cultures, showing that the overexpression indeed conveyed the desired increased reduction capacity...
  7. doi request reprint NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae
    João R M Almeida
    Department of Applied Microbiology, Lund University, Lund, Sweden
    Appl Microbiol Biotechnol 78:939-45. 2008
    ....
  8. ncbi request reprint Efficient anaerobic whole cell stereoselective bioreduction with recombinant Saccharomyces cerevisiae
    Michael Katz
    Department of Applied Microbiology, Lund University, P O Box 124, 221 00 Lund, Sweden
    Biotechnol Bioeng 84:573-82. 2003
    ..Combining overexpression of BCO2,6D reductase with reduced glycolytic rate (low phosphoglucose isomerase activity) offers a fast whole cell stereoselective bioreduction system useful for facilitated anaerobic batch conversions...
  9. ncbi request reprint The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains
    Marie Jeppsson
    Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden
    Yeast 20:1263-72. 2003
    ..Low G6PDH-activity strains were also more sensitive to H(2)O(2) than the control strain TMB3001...
  10. doi request reprint Stress-related challenges in pentose fermentation to ethanol by the yeast Saccharomyces cerevisiae
    João R M Almeida
    Applied Microbiology, Lund University, Lund, Sweden EMBRAPA Agroenergy, PqEB, Brasilia, 70770 901 DF, Brazil
    Biotechnol J 6:286-99. 2011
    ..Finally, the fermentation of a real lignocellulosic medium is discussed in terms of inhibitory effects of furaldehydes, phenolics and weak acids and the presence of contaminating microbiota...
  11. doi request reprint Variability of the response of Saccharomyces cerevisiae strains to lignocellulose hydrolysate
    Tobias Modig
    Department of Chemical Engineering, Lund University, Lund, Sweden
    Biotechnol Bioeng 100:423-9. 2008
    ....
  12. ncbi request reprint Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering
    Kaisa Karhumaa
    Department of Applied Microbiology, Lund University, PO Box 124, SE 22100 Lund, Sweden
    Yeast 22:359-68. 2005
    ..This demonstrates the necessity to simultaneously increase the conversion of xylose to xylulose and the metabolic steps downstream of xylulose for efficient xylose utilization in S. cerevisiae...
  13. doi request reprint Electrochemical probing of in vivo 5-hydroxymethyl furfural reduction in Saccharomyces cerevisiae
    Natalie V Kostesha
    Department of Applied Microbiology, Lund University, P O Box 124, SE 221 00 Lund, Sweden
    Anal Chem 81:9896-901. 2009
    ..These results demonstrate that the mediated amperometric method is useful for monitoring the short-term dynamics of NAD(P)H variations and determining cellular enzyme kinetic parameters in S. cerevisiae cells...
  14. doi request reprint Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering
    Oskar Bengtsson
    Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden
    Yeast 25:835-47. 2008
    ..The strains overexpressing SOL3 and TAL1 grew 19% and 24% faster than their reference strain, and the strains carrying deletions of YLR042C, MNI1 or RPA49 grew 173%, 62% and 90% faster than their reference strain...
  15. doi request reprint Comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli for the production of an optically pure keto alcohol
    Nádia Skorupa Parachin
    Department of Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund University, P O Box 124, 221 00, Lund, Sweden
    Appl Microbiol Biotechnol 84:487-97. 2009
    ..S. cerevisiae required an increased NADPH regeneration rate to supply YPR1 with co-enzyme while the native NADPH regeneration rate was sufficient for E. coli...
  16. ncbi request reprint Reaction and strain engineering for improved stereo-selective whole-cell reduction of a bicyclic diketone
    Ted Johanson
    Department of Applied Microbiology, Lund University, P O Box 124, SE 22100, Lund, Sweden
    Appl Microbiol Biotechnol 77:1111-8. 2008
    ....
  17. doi request reprint Carbon fluxes of xylose-consuming Saccharomyces cerevisiae strains are affected differently by NADH and NADPH usage in HMF reduction
    João R M Almeida
    Department of Applied Microbiology, Lund University, P O Box 124, S 221 00, Lund, Sweden
    Appl Microbiol Biotechnol 84:751-61. 2009
    ..In particular, NADH-dependent HMF reduction contributed to carbon conservation so that biomass was produced at the expense of xylitol and glycerol formation...
  18. ncbi request reprint High activity of xylose reductase and xylitol dehydrogenase improves xylose fermentation by recombinant Saccharomyces cerevisiae
    Kaisa Karhumaa
    Department of Applied Microbiology, Lund University, P O Box 124, SE 22100, Lund, Sweden
    Appl Microbiol Biotechnol 73:1039-46. 2007
    ..The results indicate that ethanolic xylose fermentation by recombinant S. cerevisiae expressing XR and XDH is governed by the efficiency by which xylose is introduced in the central metabolism...
  19. doi request reprint The deletion of YLR042c improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
    Nádia S Parachin
    Department of Applied Microbiology, Lund University, Sweden
    Yeast 27:741-51. 2010
    ..Altogether, these results suggest that YLR042c influences xylose and the assimilation of carbon sources other than glucose, and that the effect could be at the level of sugar transport or sugar signalling...
  20. doi request reprint Kinetic modelling reveals current limitations in the production of ethanol from xylose by recombinant Saccharomyces cerevisiae
    Nadia Skorupa Parachin
    Department of Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
    Metab Eng 13:508-17. 2011
    ..Under anaerobic conditions, the XI strains overexpressing xylB gene and the combination of xylB and GFX1 genes consumed 27% and 37% more xylose than the control strain...
  21. ncbi request reprint Effect of enhanced xylose reductase activity on xylose consumption and product distribution in xylose-fermenting recombinant Saccharomyces cerevisiae
    Marie Jeppsson
    Department of Applied Microbiology, Lund University, P O Box 124, 221 00 Lund, Sweden
    FEMS Yeast Res 3:167-75. 2003
    ..Enhanced glycerol yields were observed in the high-XR-activity strains. These are suggested to result from the observed reductase activity of the purified XR for dihydroxyacetone phosphate...
  22. doi request reprint Metabolic effects of furaldehydes and impacts on biotechnological processes
    João R M Almeida
    Department of Applied Microbiology, Lund University, P O Box 124, 221 00 Lund, Sweden
    Appl Microbiol Biotechnol 82:625-38. 2009
    ..In this short review, we take a look at known metabolic effects, as well as strategies to overcome problems in biotechnological applications caused by furaldehydes...
  23. doi request reprint Screening of Saccharomyces cerevisiae strains with respect to anaerobic growth in non-detoxified lignocellulose hydrolysate
    João R M Almeida
    Department of Applied Microbiology, Lund University, P O Box 124, S 221 00 Lund, Sweden
    Bioresour Technol 100:3674-7. 2009
    ....
  24. pmc Cofactor dependence in furan reduction by Saccharomyces cerevisiae in fermentation of acid-hydrolyzed lignocellulose
    Anneli Nilsson
    Department of Chemical Engineering, Lund University, P O Box 124, S 221 00 Lund, Sweden
    Appl Environ Microbiol 71:7866-71. 2005
    ..The ability to reduce 5-hydroxymethyl furfural is an important characteristic for the development of yeast strains with increased tolerance to lignocellulosic hydrolysates...
  25. ncbi request reprint Towards industrial pentose-fermenting yeast strains
    Bärbel Hahn-Hägerdal
    Department of Applied Microbiology, Lund University, PO Box 124, Lund 22100, Sweden
    Appl Microbiol Biotechnol 74:937-53. 2007
    ....
  26. ncbi request reprint Metabolic engineering for pentose utilization in Saccharomyces cerevisiae
    Bärbel Hahn-Hägerdal
    Applied Microbiology, Lund University, P O Box 124, 221 00, Lund, Sweden
    Adv Biochem Eng Biotechnol 108:147-77. 2007
    ..cerevisiae is primarily limited to genetically and physiologically well-characterized laboratory strains. The translation of this knowledge to strains performing in an industrial context is discussed...
  27. pmc PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae
    Rosa Garcia Sanchez
    Department of Applied Microbiology, Lund University, P O Box 124, SE 22100 Lund, Sweden
    Microb Cell Fact 9:40. 2010
    ..Overexpression of PGM2 has previously been shown to enhance aerobic growth of S. cerevisiae in galactose medium...
  28. ncbi request reprint Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400
    Karin Ohgren
    Department of Chemical Engineering, Lund University, P O Box 124, SE 221 00 Lund, Sweden
    J Biotechnol 126:488-98. 2006
    ..The ethanol productivity increased with increasing concentration of pretreatment hydrolysate in the yeast production medium and when SSF was performed in a fed-batch mode...
  29. doi request reprint Flavonoids as inhibitors of human carbonyl reductase 1
    Magnus Carlquist
    Department of Applied Microbiology, Lund University, P O Box 124, SE 221 00 Lund, Sweden
    Chem Biol Interact 174:98-108. 2008
    ..These included; hydrogen-binding sites surrounding Ser139 and Cys226, Met234 and Tyr193 or Trp229; aromatic-aromatic interaction with Tyr193, Trp229 or NADPH; van der Waals interactions with Ile140...
  30. doi request reprint Proteome analysis of the xylose-fermenting mutant yeast strain TMB 3400
    Kaisa Karhumaa
    Department of Applied Microbiology, Lund University, PO Box 124, SE 22100 Lund, Sweden
    Yeast 26:371-82. 2009
    ....
  31. ncbi request reprint Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae
    Márk Gárdonyi
    Department of Applied Microbiology, Lund University, P O Box 124, 22100 Lund, Sweden
    Biotechnol Bioeng 82:818-24. 2003
    ..5 g L(-1). However, for strain TMB3260 the flux control coefficient was higher than 0.5 at xylose concentrations < 0.6 g L(-1), while C(J) (transp) stayed below 0.2 for strain TMB3001 irrespective of xylose concentration...
  32. pmc Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway
    Maurizio Bettiga
    Department of Applied Microbiology, Lund University, PO Box 124, SE 22100 Lund, Sweden
    Microb Cell Fact 8:40. 2009
    ..abstract:..
  33. ncbi request reprint Strain engineering for stereoselective bioreduction of dicarbonyl compounds by yeast reductases
    Ted Johanson
    Department of Applied Microbiology, Lund University, P O Box 124, SE 22100 Lund, Sweden
    FEMS Yeast Res 5:513-25. 2005
    ..This review discusses two aspects of strain engineering: (i) the generation of strains with higher reductase activity towards dicarbonyl compounds and (ii) the optimisation of co-substrate utilisation for NADPH cofactor regeneration...
  34. ncbi request reprint Amperometric response from the glycolytic versus the pentose phosphate pathway in Saccharomyces cerevisiae cells
    Christer F Spégel
    Department of Analytical Chemistry, Department of Applied Microbiology, Lund University, P O Box 124, SE 22100 Lund, Sweden
    Anal Chem 79:8919-26. 2007
    ..These in vitro experiments show a higher activity of NADH-dependent than NADPH-dependent menadione-reducing dehydrogenases in S. cerevisiae cells...
  35. ncbi request reprint Mild detergent treatment of Candida tropicalis reveals a NADPH-dependent reductase in the crude membrane fraction, which enables the production of pure bicyclic exo-alcohol
    Michael Katz
    Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden
    Yeast 21:1253-67. 2004
    ..The exo-reductase is suspected to be either a beta-hydroxysteroid dehydrogenase or a polyol dehydrogenase from either the short chain dehydrogenase family or the dihydroflavonol reductase family...
  36. pmc Engineering yeast hexokinase 2 for improved tolerance toward xylose-induced inactivation
    Basti Bergdahl
    Division of Applied Microbiology, Department of Chemistry, Lund University, Lund, Sweden
    PLoS ONE 8:e75055. 2013
    ....
  37. doi request reprint Flotation as a tool for indirect DNA extraction from soil
    Nadia Skorupa Parachin
    Applied Microbiology, Lund Institute of Technology, Lund University, PO Box 124, 221 00 Lund, Sweden
    Appl Microbiol Biotechnol 87:1927-33. 2010
    ..5 microg DNA/g soil), and neither PCR nor restriction enzyme digestion of DNA were inhibited. Furthermore, specific primers enabled recovery of both prokaryotic and eukaryotic sequences...
  38. ncbi request reprint The expression of a Pichia stipitis xylose reductase mutant with higher K(M) for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae
    Marie Jeppsson
    Department of Applied Microbiology, Lund University, P O Box 124, SE 22100 Lund, Sweden
    Biotechnol Bioeng 93:665-73. 2006
    ..40 g per gram of sugar and a xylose consumption rate of 0.16 g per gram of biomass per hour in chemostat culture (0.06/h) with 10 g/L glucose and 10 g/L xylose as carbon source...
  39. ncbi request reprint Efficient bioreduction of bicyclo[2.2.2]octane-2,5-dione and bicyclo[2.2.2]oct-7-ene-2,5-dione by genetically engineered Saccharomyces cerevisiae
    Annika Friberg
    Division of Organic Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P O Box 124, SE 221 00 Lund, Sweden
    Org Biomol Chem 4:2304-12. 2006
    ..However, TMB4100 led to significantly higher conversion rates (over 40 fold faster) and also a minor improvement of the enantiomeric excesses (>99%)...
  40. pmc Short-term adaptation improves the fermentation performance of Saccharomyces cerevisiae in the presence of acetic acid at low pH
    Violeta Sànchez i Nogué
    Division of Applied Microbiology, Department of Chemistry, Lund University, P O Box 124, SE 22100 Lund, Sweden
    Appl Microbiol Biotechnol 97:7517-25. 2013
    ..7) and in the presence of inhibitory levels of acetic acid (6 g L⁻¹). During anaerobic cultivation with adapted cells of strain TMB3500, the specific ethanol production rate was increased, reducing the fermentation time to 48 %...
  41. pmc Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose
    Marie Jeppsson
    Department of Applied Microbiology, Lund University, 221 00 Lund, Sweden
    Appl Environ Microbiol 68:1604-9. 2002
    ..These results indicate that xylitol production is strongly connected to the flux through the oxidative part of the pentose phosphate pathway...