John Wahren

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi request reprint Splanchnic regulation of glucose production
    John Wahren
    Department of Molecular Medicine and Surgery, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Annu Rev Nutr 27:329-45. 2007
  2. ncbi request reprint C-peptide is a bioactive peptide
    J Wahren
    Department of Molecular Medicine and Surgery, Karolinska Hospital, 171 76, Stockholm, Sweden
    Diabetologia 50:503-9. 2007
  3. ncbi request reprint C-Peptide replacement therapy and sensory nerve function in type 1 diabetic neuropathy
    Karin Ekberg
    Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Diabetes Care 30:71-6. 2007
  4. ncbi request reprint C-peptide improves adenosine-induced myocardial vasodilation in type 1 diabetes patients
    Bo Lennart Johansson
    Department of Surgical Sciences, Division of Clinical Physiology N1 05, Karolinska Institutet, Karolinska Hospital, SE 171 76 Stockholm, Sweden
    Am J Physiol Endocrinol Metab 286:E14-9. 2004
  5. pmc Molecular and cellular effects of C-peptide--new perspectives on an old peptide
    John Wahren
    Department of Surgical Sciences, Section of Clinical Physiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Exp Diabesity Res 5:15-23. 2004
  6. ncbi request reprint Amelioration of sensory nerve dysfunction by C-Peptide in patients with type 1 diabetes
    Karin Ekberg
    Department of Surgical Sciences, Section of Clinical Physiology, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Diabetes 52:536-41. 2003
  7. ncbi request reprint C-peptide increases forearm blood flow in patients with type 1 diabetes via a nitric oxide-dependent mechanism
    Bo Lennart Johansson
    Department of Surgical Sciences, Division of Clinical Physiology N1 05, Karolinska Hospital, SE 171 76 Stockholm, Sweden
    Am J Physiol Endocrinol Metab 285:E864-70. 2003
  8. ncbi request reprint C-peptide exerts beneficial effects on myocardial blood flow and function in patients with type 1 diabetes
    Alexander Hansen
    Section of Cardiology, Department of Medicine, Karolinska Hospital, Stockholm, Sweden
    Diabetes 51:3077-82. 2002
  9. ncbi request reprint C-peptide: new findings and therapeutic implications in diabetes
    John Wahren
    Section of Clinical Physiology, Department of Surgical Sciences, Karolinska Institute, N1 05, SE 171 76 Stockholm, Sweden
    Clin Physiol Funct Imaging 24:180-9. 2004
  10. ncbi request reprint Ernst-Friedrich-Pfeiffer Memorial Lecture. New aspects of C-peptide physiology
    J Wahren
    Department of Surgical Sciences, Karolinska Hospital, Stockholm, Sweden
    Horm Metab Res 30:A2-5. 1998

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Splanchnic regulation of glucose production
    John Wahren
    Department of Molecular Medicine and Surgery, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Annu Rev Nutr 27:329-45. 2007
    ..The hyperglycemia of type 2 diabetes is in part caused by an overproduction of glucose from the liver that is secondary to accelerated gluconeogenesis...
  2. ncbi request reprint C-peptide is a bioactive peptide
    J Wahren
    Department of Molecular Medicine and Surgery, Karolinska Hospital, 171 76, Stockholm, Sweden
    Diabetologia 50:503-9. 2007
  3. ncbi request reprint C-Peptide replacement therapy and sensory nerve function in type 1 diabetic neuropathy
    Karin Ekberg
    Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Diabetes Care 30:71-6. 2007
    ..The present study was undertaken to examine whether C-peptide administration to patients with type 1 diabetes and peripheral neuropathy improves sensory nerve function...
  4. ncbi request reprint C-peptide improves adenosine-induced myocardial vasodilation in type 1 diabetes patients
    Bo Lennart Johansson
    Department of Surgical Sciences, Division of Clinical Physiology N1 05, Karolinska Institutet, Karolinska Hospital, SE 171 76 Stockholm, Sweden
    Am J Physiol Endocrinol Metab 286:E14-9. 2004
    ..05) and stroke volume (+7%, P < 0.05). It is concluded that short-term C-peptide infusion in physiological amounts increases the hyperemic MBF and left-ventricular function in type 1 diabetic patients...
  5. pmc Molecular and cellular effects of C-peptide--new perspectives on an old peptide
    John Wahren
    Department of Surgical Sciences, Section of Clinical Physiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Exp Diabesity Res 5:15-23. 2004
    ..Clinical evidence suggests that replacement of C-peptide, together with regular insulin therapy, may be beneficial in patients with type 1 diabetes and serve to retard or prevent the development of long-term complications...
  6. ncbi request reprint Amelioration of sensory nerve dysfunction by C-Peptide in patients with type 1 diabetes
    Karin Ekberg
    Department of Surgical Sciences, Section of Clinical Physiology, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Diabetes 52:536-41. 2003
    ..In conclusion, C-peptide administered for 3 months as replacement therapy to patients with early signs of diabetic neuropathy ameliorates nerve dysfunction...
  7. ncbi request reprint C-peptide increases forearm blood flow in patients with type 1 diabetes via a nitric oxide-dependent mechanism
    Bo Lennart Johansson
    Department of Surgical Sciences, Division of Clinical Physiology N1 05, Karolinska Hospital, SE 171 76 Stockholm, Sweden
    Am J Physiol Endocrinol Metab 285:E864-70. 2003
    ..It is concluded that the stimulatory effect of C-peptide on FBF in type 1 diabetic patients is mediated via the NO system and that C-peptide increases basal endothelial NO levels...
  8. ncbi request reprint C-peptide exerts beneficial effects on myocardial blood flow and function in patients with type 1 diabetes
    Alexander Hansen
    Section of Cardiology, Department of Medicine, Karolinska Hospital, Stockholm, Sweden
    Diabetes 51:3077-82. 2002
    ..3 +/- 0.4 to 5.3 +/- 0.9 (P < 0.05). The results demonstrate that type 1 diabetic patients have impaired myocardial function and perfusion in the basal state that can be improved by short-term replacement of C-peptide...
  9. ncbi request reprint C-peptide: new findings and therapeutic implications in diabetes
    John Wahren
    Section of Clinical Physiology, Department of Surgical Sciences, Karolinska Institute, N1 05, SE 171 76 Stockholm, Sweden
    Clin Physiol Funct Imaging 24:180-9. 2004
    ....
  10. ncbi request reprint Ernst-Friedrich-Pfeiffer Memorial Lecture. New aspects of C-peptide physiology
    J Wahren
    Department of Surgical Sciences, Karolinska Hospital, Stockholm, Sweden
    Horm Metab Res 30:A2-5. 1998
    ..In conclusion, the combined findings indicate that C-peptide is a biologically active hormone. The possibility that C-peptide therapy in IDDM patients may be beneficial should be considered...
  11. pmc Large-surface biosensor technology for enhanced recovery in protein characterization
    Ermias Melles
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    J Biomol Tech 16:392-7. 2005
    ..Recovery of affinity partners at the multi-pmole level is demonstrated for protein purification in SPR approaches...
  12. ncbi request reprint Molecular effects of proinsulin C-peptide
    Jan Johansson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 295:1035-40. 2002
    ..Combined, the available observations show that C-peptide is biologically active and suggest a molecular model for its physiological effects...
  13. pmc Proinsulin C-peptide elicits disaggregation of insulin resulting in enhanced physiological insulin effects
    J Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Cell Mol Life Sci 63:1805-11. 2006
    ..It is compatible with the normal co-release of C-peptide and insulin and may contribute to the beneficial effect of C-peptide and insulin replacement in type 1 diabetics...
  14. pmc C-peptide increases Na,K-ATPase expression via PKC- and MAP kinase-dependent activation of transcription factor ZEB in human renal tubular cells
    Dana Galuska
    Section of Integrative Physiology, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 6:e28294. 2011
    ..We determined the molecular mechanism by which long term exposure to C-peptide stimulates Na,K-ATPase expression and activity in primary human renal tubular cells (HRTC) in control and hyperglycemic conditions...
  15. doi request reprint Precise determination of glucose-d(2)/glucose ratio in human serum and plasma by APCI LC-MS/MS
    Shahid Ullah
    Department of Medicine, Division of Clinical Pharmacology, Karolinska Institute and University Hospital, Stockholm, Sweden
    Scand J Clin Lab Invest 69:837-42. 2009
    ..Determinations of glucose turnover based on infusion of deuterium-labelled glucose are frequently undertaken in studies involving the pathophysiology of diabetes or obesity or the metabolic response to physical exercise...
  16. ncbi request reprint C-peptide: a new potential in the treatment of diabetic nephropathy
    J Wahren
    Department of Surgical Sciences, Karolinska Hospital, Stockholm SE 171 76, Sweden
    Curr Diab Rep 1:261-6. 2001
    ..C-peptide replacement together with insulin therapy may be beneficial in type 1 diabetes patients with nephropathy...
  17. ncbi request reprint Role of C-peptide in human physiology
    J Wahren
    Department of Surgical Sciences, Section of Clinical Physiology, Karolinska Hospital, SE 171 76 Stockholm, Sweden
    Am J Physiol Endocrinol Metab 278:E759-68. 2000
    ..The possibility exists that C-peptide replacement, together with insulin administration, may prevent the development or retard the progression of long-term complications in type 1 diabetes...
  18. ncbi request reprint Cellular internalization of proinsulin C-peptide
    E Lindahl
    Department of Medical Biochemistry and Biophysics MBB, Karolinska Institutet, 171 77, Stockholm, Sweden
    Cell Mol Life Sci 64:479-86. 2007
    ..Hence, the data suggest the possibility of one further C-peptide site of action...
  19. ncbi request reprint C-peptide binding to human cell membranes: importance of Glu27
    A Pramanik
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SE-171 77, Sweden
    Biochem Biophys Res Commun 284:94-8. 2001
    ..These results indicate that Glu27 of C-peptide is critically involved in binding to cellular targets...
  20. ncbi request reprint Diminished skin blood flow in Type I diabetes: evidence for non-endothelium-dependent dysfunction
    A Katz
    Department of Surgical Sciences, Division of Clinical Physiology, Karolinska Institute, Karolinska Hospital, 171 76 Stockholm, Sweden
    Clin Sci (Lond) 101:59-64. 2001
    ..These data demonstrate that Type I diabetic patients have similar diminished SBF responses to iontophoresis of ACh and SNP, which suggests that non-endothelial-dependent factors are primarily responsible for the diminished SBF responses...
  21. ncbi request reprint Proinsulin C-peptide and its analogues induce intracellular Ca2+ increases in human renal tubular cells
    J Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Cell Mol Life Sci 59:1185-9. 2002
    ..Combined, all data show that C-peptide is a bioactive peptide and suggest that it elicits changes in [Ca2+]i via G-protein-coupled pathways, giving downstream enzyme effects...
  22. ncbi request reprint Separate functional features of proinsulin C-peptide
    M Henriksson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77, Stockholm, Sweden
    Cell Mol Life Sci 62:1772-8. 2005
    ..Previous findings have ascribed functional roles to the C-terminal pentapeptide segment, and all results combined therefore now show the importance of different segments, suggesting that C-peptide interactions are complex or multiple...
  23. ncbi request reprint Unordered structured of proinsulin C-peptide in aqueous solution and in the presence of lipid vesicles
    M Henriksson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Cell Mol Life Sci 57:337-42. 2000
    ..In conclusion, C-peptide is unlikely to elicit physiological effects through stable conformation-dependent interactions with lipid membranes...
  24. ncbi request reprint C-peptide stimulates ERK1/2 and JNK MAP kinases via activation of protein kinase C in human renal tubular cells
    Z Zhong
    Section of Clinical Physiology, Department of Surgical Sciences, Karolinska Institute, Stockholm, Sweden
    Diabetologia 48:187-97. 2005
    ..This study examines the intracellular signalling pathways activated by C-peptide in human renal tubular cells...
  25. ncbi request reprint Insulin binding monitored by fluorescence correlation spectroscopy
    Z H Zhong
    Department of Surgical Sciences, Karolinska Hospital, Stockholm, Sweden
    Diabetologia 44:1184-8. 2001
    ..Because of its high sensitivity (single molecule detection), FCS, provides additional data allowing a more precise evaluation of the kinetics of ligand-receptor interactions at low expression levels in living cells...
  26. ncbi request reprint C-peptide improves neuropathy in type 1 diabetic BB/Wor-rats
    Weixian Zhang
    Department of Pathology, Wayne State University, School of Medicine, Detroit, MI 48201, USA
    Diabetes Metab Res Rev 23:63-70. 2007
    ..Here we examine the therapeutic effects of C-peptide, delivered as continuous infusion or once daily subcutaneous injections on established DPN...
  27. ncbi request reprint C-Peptide reverses nociceptive neuropathy in type 1 diabetes
    Hideki Kamiya
    Department of Pathology, Scott Hall 9275, 540 E Canfield Ave, Detroit, MI 48201, USA
    Diabetes 55:3581-7. 2006
    ..We conclude that C-peptide exerts beneficial therapeutic effects on diabetic nociceptive neuropathy and that optimal effects require maintenance of physiological C-peptide concentrations for a major proportion of the day...
  28. ncbi request reprint Direct measurements of the permeability surface area for insulin and glucose in human skeletal muscle
    Soffia Gudbjornsdottir
    Lundberg Laboratory for Diabetes Research, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
    J Clin Endocrinol Metab 88:4559-64. 2003
    ....
  29. ncbi request reprint C-peptide makes a comeback
    John Wahren
    Diabetes Metab Res Rev 19:345-7. 2003
    ..The possibility that replacement of C-peptide in patients with type 1 diabetes may serve to retard or prevent the development of long-term complications should be evaluated...
  30. pmc New tricks by an old dog
    Thomas Forst
    Exp Diabetes Res 2008:384219. 2008
  31. pmc C-peptide and its C-terminal fragments improve erythrocyte deformability in type 1 diabetes patients
    Thomas Hach
    Department of Internal Medicine, University of Mainz, 55101 Mainz, Germany thomas
    Exp Diabetes Res 2008:730594. 2008
    ..This study aimed to investigate the influence of C-peptide and fragments thereof on erythrocyte deformability and to elucidate the relevant signal transduction pathway...
  32. ncbi request reprint In vivo biodistribution and pharmacokinetics of (18)F-labeled human C-peptide: evaluation in monkeys using positron emission tomography
    Anna Fredriksson
    Karolinska Pharmacy, Karolinska Hospital and Institute, SE 171 76 Stockholm, Sweden
    Life Sci 71:1361-70. 2002
    ..The organs with the highest radioactivity concentrations have been identified, enabling dose estimations for studies in humans with low or no C-peptide...
  33. ncbi request reprint C-peptide and captopril are equally effective in lowering glomerular hyperfiltration in diabetic rats
    Björn Samnegård
    Department of Nephrology, Danderyd Hospital, SE 182 88 Stockholm, Sweden
    Nephrol Dial Transplant 19:1385-91. 2004
    ....
  34. ncbi request reprint Influence of C-peptide on early glomerular changes in diabetic mice
    Yoshiro Maezawa
    Department of Clinical Cell Biology and Medicine, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chiba 260 8670, Japan
    Diabetes Metab Res Rev 22:313-22. 2006
    ..This study aims to examine the molecular effects of C-peptide on early glomerular changes in a mouse model of type 1 diabetes...
  35. ncbi request reprint The contribution of visceral adipose tissue to splanchnic cortisol production in healthy humans
    Ruth Andrew
    Endocrinology Unit, School of Molecular and Clinical Medicine, University of Edinburgh, Western General Hospital, Edinburgh, EH4 2XU, UK
    Diabetes 54:1364-70. 2005
    ..Thus, in addition to free fatty acids and adipokines, the portal vein delivers cortisol to the liver, and inhibition of 11HSD1 in visceral adipose tissue may indeed be valuable in ameliorating insulin resistance in obesity...
  36. ncbi request reprint C-peptide prevents glomerular hypertrophy and mesangial matrix expansion in diabetic rats
    Björn Samnegård
    Department of Nephrology, Danderyd Hospital, SE 182 88 Stockholm, Sweden
    Nephrol Dial Transplant 20:532-8. 2005
    ..The aim of this study was to clarify further the effects of C-peptide on renal structural changes in type-1 diabetic rats...
  37. ncbi request reprint Effects of proinsulin C-peptide in experimental diabetic neuropathy: vascular actions and modulation by nitric oxide synthase inhibition
    Mary A Cotter
    Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Scotland, UK
    Diabetes 52:1812-7. 2003
    ..Thus, C-peptide replacement improves nerve function in experimental diabetes, and the data are compatible with the notion that this is mediated by a NO-sensitive vascular mechanism...