Nicola Solaroli

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. pmc Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design
    Kamel El Omari
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
    BMC Struct Biol 6:22. 2006
  2. doi Identification of two active functional domains of human adenylate kinase 5
    Nicola Solaroli
    Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden
    FEBS Lett 583:2872-6. 2009
  3. doi Substrate specificity of feline and canine herpesvirus thymidine kinase
    N Solaroli
    Department of Laboratory Medicine, Karolinska Institute, S 141 86 Stockholm, Sweden
    Antiviral Res 79:128-32. 2008
  4. ncbi Mitochondrial expression of the Drosophila melanogaster multisubstrate deoxyribonucleoside kinase
    Nicola Solaroli
    Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Virology F68, S 14186 Stockholm, Sweden
    Mol Pharmacol 72:1593-8. 2007
  5. ncbi Enhanced toxicity of purine nucleoside analogs in cells expressing Drosophila melanogaster nucleoside kinase mutants
    N Solaroli
    Department of Laboratory Medicine, Division of Metabolic Diseases, Karolinska Institute, Stockholm, Sweden
    Gene Ther 14:86-92. 2007
  6. ncbi Substrate specificity of three viral thymidine kinases (TK): vaccinia virus TK, feline herpesvirus TK, and canine herpesvirus TK
    N Solaroli
    Mitochondrial Medicine Center, Karolinska Institutet, Stockholm, Sweden
    Nucleosides Nucleotides Nucleic Acids 25:1189-92. 2006
  7. doi Evidence of an intact N-terminal translocation sequence of human mitochondrial adenylate kinase 4
    Christakis Panayiotou
    Department of Laboratory Medicine, Karolinska Institute, F68, S 141 86 Huddinge, Sweden
    Int J Biochem Cell Biol 42:62-9. 2010
  8. doi Retained sensitivity to cytotoxic pyrimidine nucleoside analogs in thymidine kinase 2 deficient human fibroblasts
    Mia Bjerke
    Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden
    Nucleosides Nucleotides Nucleic Acids 29:1-13. 2010
  9. doi The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes
    Christakis Panayiotou
    Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden
    Biochem J 433:527-34. 2011
  10. doi Hematopoiesis in the thymidine kinase 2 deficient mouse model of mitochondrial DNA depletion syndrome
    Xiaoshan Zhou
    Clinical Microbiology F68, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden
    J Inherit Metab Dis 33:231-6. 2010

Collaborators

Detail Information

Publications19

  1. pmc Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design
    Kamel El Omari
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
    BMC Struct Biol 6:22. 2006
    ..Vaccinia virus TK is a close orthologue of variola TK but also shares a relatively high sequence identity to human type 2 TK (hTK), thus achieving drug selectivity relative to the host enzyme is challenging...
  2. doi Identification of two active functional domains of human adenylate kinase 5
    Nicola Solaroli
    Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden
    FEBS Lett 583:2872-6. 2009
    ..The first domain, AK5p1, phosphorylated AMP, CMP, dAMP and dCMP with ATP or GTP as phosphate donors similarly to AK5p2. Our data demonstrate that human AK5 has two separate functional domains and that both have enzymatic activity...
  3. doi Substrate specificity of feline and canine herpesvirus thymidine kinase
    N Solaroli
    Department of Laboratory Medicine, Karolinska Institute, S 141 86 Stockholm, Sweden
    Antiviral Res 79:128-32. 2008
    ..The reason is likely the poor, if any, thymidylate kinase activity of FHV and CHV TK, which in HSV-1 TK-expressing cells convert BVDU-MP to its 5'-diphosphate derivative...
  4. ncbi Mitochondrial expression of the Drosophila melanogaster multisubstrate deoxyribonucleoside kinase
    Nicola Solaroli
    Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Virology F68, S 14186 Stockholm, Sweden
    Mol Pharmacol 72:1593-8. 2007
    ....
  5. ncbi Enhanced toxicity of purine nucleoside analogs in cells expressing Drosophila melanogaster nucleoside kinase mutants
    N Solaroli
    Department of Laboratory Medicine, Division of Metabolic Diseases, Karolinska Institute, Stockholm, Sweden
    Gene Ther 14:86-92. 2007
    ..In summary, our data show that enzyme engineering can be used to shift the substrate specificity of the Dm-dNK to selectively increase the sensitivity of cells expressing the enzyme to purine nucleoside analogs...
  6. ncbi Substrate specificity of three viral thymidine kinases (TK): vaccinia virus TK, feline herpesvirus TK, and canine herpesvirus TK
    N Solaroli
    Mitochondrial Medicine Center, Karolinska Institutet, Stockholm, Sweden
    Nucleosides Nucleotides Nucleic Acids 25:1189-92. 2006
    ....
  7. doi Evidence of an intact N-terminal translocation sequence of human mitochondrial adenylate kinase 4
    Christakis Panayiotou
    Department of Laboratory Medicine, Karolinska Institute, F68, S 141 86 Huddinge, Sweden
    Int J Biochem Cell Biol 42:62-9. 2010
    ..Site directed mutagenesis of amino acids Lys 4 and Arg 7 showed that these two residues were essential for mitochondrial import...
  8. doi Retained sensitivity to cytotoxic pyrimidine nucleoside analogs in thymidine kinase 2 deficient human fibroblasts
    Mia Bjerke
    Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden
    Nucleosides Nucleotides Nucleic Acids 29:1-13. 2010
    ..This study suggests that nucleoside analog phosphorylation mediated by TK2 may be less important, compared to other deoxyribonucleoside kinases, for the cytotoxic effects of these compounds...
  9. doi The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes
    Christakis Panayiotou
    Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden
    Biochem J 433:527-34. 2011
    ..Expression of the proteins fused with green fluorescent protein demonstrated a cytosolic localization for both AK7 and AK8...
  10. doi Hematopoiesis in the thymidine kinase 2 deficient mouse model of mitochondrial DNA depletion syndrome
    Xiaoshan Zhou
    Clinical Microbiology F68, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden
    J Inherit Metab Dis 33:231-6. 2010
    ....
  11. ncbi Investigation of the substrate recognition of Drosophila melanogaster nucleoside kinase by site directed mutagenesis
    N Solaroli
    Division of Clinical Virology F68, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
    Nucleosides Nucleotides Nucleic Acids 23:1527-9. 2004
    ..We have performed site directed mutagenesis and tested different truncated forms of the enzyme in order to increase the affinity for ganciclovir...
  12. doi Two novel mutations in thymidine kinase-2 cause early onset fatal encephalomyopathy and severe mtDNA depletion
    Nicole Lesko
    Department of Laboratory Medicine, Division of Metabolic Diseases, Karolinska Institutet, Stockholm, Sweden
    Neuromuscul Disord 20:198-203. 2010
    ..We conclude that the severe clinical presentation in this patient was due to a virtual lack of mitochondrial TK2 activity...
  13. ncbi Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase
    Nicola Solaroli
    Division of Clinical Virology F68, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden and Dipartimento di Scienze Farmaceutiche, Universita di Ferrara, Italy
    Eur J Biochem 270:2879-84. 2003
    ....
  14. doi Progressive loss of mitochondrial DNA in thymidine kinase 2-deficient mice
    Xiaoshan Zhou
    Department of Laboratory Medicine, Karolinska Institute, S 141 86 Huddinge, Sweden
    Hum Mol Genet 17:2329-35. 2008
    ..We conclude that Tk2 has a major role in supplying deoxyribonucleotides for mtDNA replication and that other pathways of deoxyribonucleotide synthesis cannot compensate for loss of this enzyme...
  15. doi The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase
    Marjan Amiri
    Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institute, F68, SE 141 86 Huddinge, Sweden
    Int J Biochem Cell Biol 45:925-31. 2013
    ..These enzymes are accordingly suggested to be regarded as nucleoside mono- and diphosphate kinases with catalytic activities possibly determined by local substrate concentrations...
  16. ncbi Novel selective human mitochondrial kinase inhibitors: design, synthesis and enzymatic activity
    Nunzia Ciliberti
    Department of Pharmaceutical Sciences, University of Ferrara, Italy
    Bioorg Med Chem 15:3065-81. 2007
    ..The biological activity of a representative compound, 2'-O-decanoyl-BVaraU, was also investigated in normal human fibroblasts and was found to impair mitochondrial function due to TK2 inhibition...
  17. ncbi Engineering of a single conserved amino acid residue of herpes simplex virus type 1 thymidine kinase allows a predominant shift from pyrimidine to purine nucleoside phosphorylation
    Jan Balzarini
    Rega Institute for Medical Research, K U Leuven, B 3000 Leuven, Belgium
    J Biol Chem 281:19273-9. 2006
    ....
  18. ncbi Design and synthesis of phosphonoacetic acid (PPA) ester and amide bioisosters of ribofuranosylnucleoside diphosphates as potential ribonucleotide reductase inhibitors and evaluation of their enzyme inhibitory, cytostatic and antiviral activity
    Stefano Manfredini
    Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy
    Antivir Chem Chemother 14:183-94. 2003
    ....
  19. ncbi Design, synthesis and anti flaviviridae activity of N(6)-, 5',3'-O- and 5',2'-O-substituted adenine nucleoside analogs
    Angela Angusti
    Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy
    Chem Pharm Bull (Tokyo) 56:423-32. 2008
    ..A molecular modeling study was also carried out to highlight the possible interactions between this compounds class and the corresponding hepatitis C virus (HCV) enzyme...