Ute Romling

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. pmc Complex c-di-GMP signaling networks mediate transition between virulence properties and biofilm formation in Salmonella enterica serovar Typhimurium
    Irfan Ahmad
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 6:e28351. 2011
  2. pmc Cyclic di-GMP: the first 25 years of a universal bacterial second messenger
    Ute Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Microbiol Mol Biol Rev 77:1-52. 2013
  3. doi request reprint Biofilm infections, their resilience to therapy and innovative treatment strategies
    U Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    J Intern Med 272:541-61. 2012
  4. doi request reprint Cyclic di-GMP, an established secondary messenger still speeding up
    Ute Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Environ Microbiol 14:1817-29. 2012
  5. pmc Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy
    Kristina Jonas
    Swedish Institute for Infectious Disease Control, Solna, Sweden
    BMC Microbiol 7:70. 2007
  6. doi request reprint Prevailing concepts of c-di-GMP signaling
    Ute Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Contrib Microbiol 16:161-81. 2009
  7. ncbi request reprint Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea
    Ute Romling
    Karolinska Institutet, Department of Microbiology, Tumor and Cell Biology, FE280, SE 171 77 Stockholm, Sweden
    Sci Signal 1:pe39. 2008
  8. ncbi request reprint Cyclic di-GMP as a second messenger
    Ute Romling
    Karolinska Institutet, Microbiology and Tumor Biology Center MTC, Box 280, SE 171 77 Stockholm, Sweden
    Curr Opin Microbiol 9:218-28. 2006
  9. ncbi request reprint Hierarchical involvement of various GGDEF domain proteins in rdar morphotype development of Salmonella enterica serovar Typhimurium
    Abdul Kader
    Microbiology and Tumor Biology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    Mol Microbiol 60:602-16. 2006
  10. ncbi request reprint The EAL-like protein STM1697 regulates virulence phenotypes, motility and biofilm formation in Salmonella typhimurium
    Irfan Ahmad
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 17177, Stockholm, Sweden
    Mol Microbiol 90:1216-32. 2013

Collaborators

Detail Information

Publications42

  1. pmc Complex c-di-GMP signaling networks mediate transition between virulence properties and biofilm formation in Salmonella enterica serovar Typhimurium
    Irfan Ahmad
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 6:e28351. 2011
    ..Our findings show that c-di-GMP signaling is at least equally important in the regulation of Salmonella-host interaction as in the regulation of biofilm formation at ambient temperature...
  2. pmc Cyclic di-GMP: the first 25 years of a universal bacterial second messenger
    Ute Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Microbiol Mol Biol Rev 77:1-52. 2013
    ..We also identify unresolved questions and highlight new directions in c-di-GMP research that will give us a deeper understanding of this truly universal bacterial second messenger...
  3. doi request reprint Biofilm infections, their resilience to therapy and innovative treatment strategies
    U Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    J Intern Med 272:541-61. 2012
    ..Their conserved structures sensed by the eukaryotic host have a widespread effect on the immune system. Thus, cyclic di-nucleotides are also potential immunotherapeutic agents to treat antibiotic-resistant bacterial infections...
  4. doi request reprint Cyclic di-GMP, an established secondary messenger still speeding up
    Ute Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Environ Microbiol 14:1817-29. 2012
    ....
  5. pmc Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy
    Kristina Jonas
    Swedish Institute for Infectious Disease Control, Solna, Sweden
    BMC Microbiol 7:70. 2007
    ....
  6. doi request reprint Prevailing concepts of c-di-GMP signaling
    Ute Romling
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Contrib Microbiol 16:161-81. 2009
    ..However, the exact molecular mechanisms of c-di-GMP action remain to be discovered...
  7. ncbi request reprint Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea
    Ute Romling
    Karolinska Institutet, Department of Microbiology, Tumor and Cell Biology, FE280, SE 171 77 Stockholm, Sweden
    Sci Signal 1:pe39. 2008
    ..To elucidate the biological importance and molecular mechanisms of action for c-di-AMP and the recently recognized second messenger c-di-GMP will require a multidisciplinary approach...
  8. ncbi request reprint Cyclic di-GMP as a second messenger
    Ute Romling
    Karolinska Institutet, Microbiology and Tumor Biology Center MTC, Box 280, SE 171 77 Stockholm, Sweden
    Curr Opin Microbiol 9:218-28. 2006
    ..Besides its role as an intracellular signaling molecule in bacteria, c-di-GMP also acts as an intercellular signaling molecule between prokaryotes and also has effects in eukaryotes that could provide a perspective in cancer treatment...
  9. ncbi request reprint Hierarchical involvement of various GGDEF domain proteins in rdar morphotype development of Salmonella enterica serovar Typhimurium
    Abdul Kader
    Microbiology and Tumor Biology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    Mol Microbiol 60:602-16. 2006
    ..Thus in the regulatory cascade leading to rdar morphotype expression c-di-GMP activates several subsequent steps in the network...
  10. ncbi request reprint The EAL-like protein STM1697 regulates virulence phenotypes, motility and biofilm formation in Salmonella typhimurium
    Irfan Ahmad
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 17177, Stockholm, Sweden
    Mol Microbiol 90:1216-32. 2013
    ..typhimurium, which are all involved in motility, biofilm and virulence regulation through interaction with proteins that regulate flagella function. ..
  11. ncbi request reprint Cyclic di-GMP signalling controls virulence properties of Salmonella enterica serovar Typhimurium at the mucosal lining
    Agaristi Lamprokostopoulou
    Department of Microbiology, Tumor and Cell Biology, FE number 280, Karolinska Institutet, 17177 Stockholm, Sweden
    Environ Microbiol 12:40-53. 2010
    ..Our results suggest that transition between biofilm formation and virulence in S. typhimurium at the epithelial cell lining is mediated by c-di-GMP signalling through CsgD and cellulose expression...
  12. pmc Complex regulatory network encompassing the Csr, c-di-GMP and motility systems of Salmonella Typhimurium
    Kristina Jonas
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Environ Microbiol 12:524-40. 2010
    ..Altogether, our data reveal that the concerted and complex regulation of several genes encoding GGDEF/EAL domain proteins allows CsrA to control the motility-sessility switch in S. Typhimurium at multiple levels...
  13. ncbi request reprint Unphosphorylated CsgD controls biofilm formation in Salmonella enterica serovar Typhimurium
    Katherina Zakikhany
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, FE 280, 17177 Stockholm, Sweden
    Mol Microbiol 77:771-86. 2010
    ..In summary, our findings suggest that the response regulator CsgD functions in its unphosphorylated form under the conditions of biofilm formation investigated in this study...
  14. ncbi request reprint GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility
    Roger Simm
    Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    Mol Microbiol 53:1123-34. 2004
    ..Thus, the data suggest that c-di-GMP is a novel global second messenger in bacteria the metabolism of which is controlled by GGDEF and EAL domain proteins...
  15. doi request reprint Characterization of cellulose production in Escherichia coli Nissle 1917 and its biological consequences
    Claudia Monteiro
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
    Environ Microbiol 11:1105-16. 2009
    ..The role of cellulose in this strain is in contrast to the role of cellulose in the commensal strain E. coli TOB1. Consequently, the role of cellulose in bacterial-host interaction is dependent on the E. coli strain background...
  16. ncbi request reprint Flagellin in combination with curli fimbriae elicits an immune response in the gastrointestinal epithelial cell line HT-29
    Maike Rochon
    Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    Microbes Infect 8:2027-33. 2006
    ..Typhimurium and Escherichia coli strains can bind flagellin and thus elicit an immune response by the intestinal epithelial cell line HT-29...
  17. pmc The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins
    Kristina Jonas
    Swedish Institute for Infectious Disease Control, SE 17182, Solna and Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, SE 17177 Stockholm, Sweden
    Mol Microbiol 70:236-57. 2008
    ..All together, these data demonstrate a global role for CsrA in the regulation of c-di-GMP metabolism by regulating the expression of GGDEF proteins at the post-transcriptional level...
  18. pmc Bistable expression of CsgD in biofilm development of Salmonella enterica serovar typhimurium
    Nina Grantcharova
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Nobels vag 16, Stockholm, Sweden
    J Bacteriol 192:456-66. 2010
    ..Consequently, bistability at the level of CsgD expression leads to a corresponding pattern of task distribution in S. Typhimurium biofilms...
  19. pmc A role for the EAL-like protein STM1344 in regulation of CsgD expression and motility in Salmonella enterica serovar Typhimurium
    Roger Simm
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    J Bacteriol 191:3928-37. 2009
    ..Consequently, although STM1344 has not retained a direct role in c-di-GMP metabolism, it still participates in the regulation of c-di-GMP turnover and has a role in the transition between sessility and motility...
  20. pmc Production of cellulose and curli fimbriae by members of the family Enterobacteriaceae isolated from the human gastrointestinal tract
    Xhavit Zogaj
    Microbiology and Tumorbiology Center, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Infect Immun 71:4151-8. 2003
    ..and Klebsiella spp. are frequently found to cause biofilm-related infections such as catheter-associated urinary tract infections, the human gut could serve as a reservoir for dissemination of biofilm-forming isolates...
  21. doi request reprint Characteristics of biofilms from urinary tract catheters and presence of biofilm-related components in Escherichia coli
    Xiaoda Wang
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, FE 280, SE 171 77, Stockholm, Sweden
    Curr Microbiol 60:446-53. 2010
    ....
  22. doi request reprint Hfq and Hfq-dependent small RNAs are major contributors to multicellular development in Salmonella enterica serovar Typhimurium
    Claudia Monteiro
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
    RNA Biol 9:489-502. 2012
    ..curli fimbriae surface-attachment at ambient temperature. Consequently, ArcZ is a major regulator of rdar biofilm development...
  23. doi request reprint Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry
    Roger Simm
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    Anal Biochem 386:53-8. 2009
    ..We describe the use of the methodology to assess the change in c-di-GMP concentration during the growth phase and the contribution of a point mutation in S. typhimurium to the overall cellular c-di-GMP concentration...
  24. ncbi request reprint The role of c-di-GMP signaling in an Aeromonas veronii biovar sobria strain
    Mokhlasur Rahman
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    FEMS Microbiol Lett 273:172-9. 2007
    ..On the other hand, overexpression of the EAL domain protein YhjH decreased biofilm formation and increased motility...
  25. pmc Role of EAL-containing proteins in multicellular behavior of Salmonella enterica serovar Typhimurium
    Roger Simm
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    J Bacteriol 189:3613-23. 2007
    ..Consequently, this work has shown that several EAL and GGDEF-EAL domain proteins, which act as phosphodiesterases, play a determinative role in the expression level of multicellular behavior of Salmonella enterica serovar Typhimurium...
  26. ncbi request reprint Regulatory components at the csgD promoter--additional roles for OmpR and integration host factor and role of the 5' untranslated region
    Ulrich Gerstel
    Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
    FEMS Microbiol Lett 261:109-17. 2006
    ..Furthermore, the role of the 174 bp long 5'-untranslated region on transcriptional activity was defined...
  27. pmc Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37
    Ylva Kai-Larsen
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS Pathog 6:e1001010. 2010
    ..Taken together, our data demonstrate that biofilm components are involved in the pathogenesis of urinary tract infections by E. coli and can be a target of local immune defense mechanisms...
  28. ncbi request reprint The csgD promoter, a control unit for biofilm formation in Salmonella typhimurium
    Ulrich Gerstel
    Microbiology and Tumorbiology Center MTC, Karolinska Institute, Stockholm, Sweden
    Res Microbiol 154:659-67. 2003
    ..Complex nucleoprotein arrangements modulate the transcriptional activity of csgD and trigger the transition between the planktonic status and biofilm formation...
  29. ncbi request reprint Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung
    Dinesh D Sriramulu
    Microbiology and Tumor Biology Center, Karolinska Institutet, 17177 Stockholm, Sweden
    J Med Microbiol 54:667-76. 2005
    ..Thus, growth of P. aeruginosa in artificial sputum medium is an appropriate model of chronic lung colonization and may be useful for evaluating therapeutic procedures and studying antibiotic-resistance mechanisms...
  30. ncbi request reprint C-di-GMP: the dawning of a novel bacterial signalling system
    Ute Romling
    Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, SE 17177 Stockholm, Sweden
    Mol Microbiol 57:629-39. 2005
    ..The accumulated data clearly indicate that a novel ubiquitous signalling system in bacteria has been discovered...
  31. doi request reprint Regulation of c-di-GMP metabolism in biofilms
    Kristina Jonas
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Future Microbiol 4:341-58. 2009
    ....
  32. pmc Regulation of biofilm components in Salmonella enterica serovar Typhimurium by lytic transglycosylases involved in cell wall turnover
    Claudia Monteiro
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, FE28017177, Stockholm, Sweden
    J Bacteriol 193:6443-51. 2011
    ..These findings reveal, for the first time, a specific function of lytic transglycosylases in S. Typhimurium and suggest the existence of a new signaling pathway that links cell wall turnover to biofilm formation...
  33. pmc Opposing contributions of polynucleotide phosphorylase and the membrane protein NlpI to biofilm formation by Salmonella enterica serovar Typhimurium
    Syed Fazle Rouf
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    J Bacteriol 193:580-2. 2011
    ..Polynucleotide phosphorylase and NlpI also had opposite effects on the expression of yjcC, which codes for a cyclic-3',5'-di-guanylate phosphodiesterase affecting CsgD expression...
  34. ncbi request reprint Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment
    Dinesh Diraviam Sriramulu
    Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
    Proteomics 5:3712-21. 2005
    ..Altogether, our results elucidate adaptive strategies of P. aeruginosa towards the nutrient-rich CF lung habitat during the course of chronic colonization...
  35. ncbi request reprint Worldwide distribution of Pseudomonas aeruginosa clone C strains in the aquatic environment and cystic fibrosis patients
    Ute Romling
    Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Stockholm, Sweden
    Environ Microbiol 7:1029-38. 2005
    ..Elucidation of the mechanisms which lead to the prevalence of clone C strain in patients and the environment requires the investigation of additional phenotypes...
  36. ncbi request reprint Occurrence and regulation of the multicellular morphotype in Salmonella serovars important in human disease
    Ute Romling
    Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden
    Int J Med Microbiol 293:273-85. 2003
    ..Therefore, the expression pattern of the rdar morphotype is serovar specific and correlates with a disease phenotype breaching the intestinal epithelial cell lining...
  37. pmc Characterization of biofilm formation and the role of BCR1 in clinical isolates of Candida parapsilosis
    Srisuda Pannanusorn
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Eukaryot Cell 13:438-51. 2014
    ..parapsilosis is both dependent and independent of BCR1, but even in strains which showed a BCR1-independent biofilm phenotype, BCR1 has alternative physiological functions. ..
  38. doi request reprint Control of pathogen growth and biofilm formation using a urinary catheter that releases antimicrobial nitrogen oxides
    Hiroaki Kishikawa
    Department of Physiology and Pharmacology, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Free Radic Biol Med 65:1257-64. 2013
    ..Although promising, future studies will reveal if this novel approach can be clinically useful for the prevention of catheter-associated urinary tract infections. ..
  39. doi request reprint Prevalence of biofilm formation in clinical isolates of Candida species causing bloodstream infection
    Srisuda Pannanusorn
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Mycoses 56:264-72. 2013
    ..These results suggest that biofilm formation as a virulence factor might have a higher significance for non-albicans Candida species than for C. albicans...
  40. ncbi request reprint Finally! The structural secrets of a HD-GYP phosphodiesterase revealed
    Edvard Wigren
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Mol Microbiol 91:1-5. 2014
    ....
  41. ncbi request reprint Complex regulation of csgD promoter activity by global regulatory proteins
    Ulrich Gerstel
    Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden
    Mol Microbiol 49:639-54. 2003
    ..A complex regulatory network involving IHF, H-NS and OmpR is proposed whereby the nucleo-complex composition in the csgD-csgBA intergenic region is altered in response to oxygen tension...