Research Topics
Genomes and Genes | Ute RomlingSummaryAffiliation: Karolinska Institutet Country: Sweden Publications
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Publications
Cyclic di-GMP: the first 25 years of a universal bacterial second messengerUte Romling
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Microbiol Mol Biol Rev 77:1-52. 2013..We also identify unresolved questions and highlight new directions in c-di-GMP research that will give us a deeper understanding of this truly universal bacterial second messenger...
Biofilm infections, their resilience to therapy and innovative treatment strategiesU Romling
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
J Intern Med 272:541-61. 2012..Their conserved structures sensed by the eukaryotic host have a widespread effect on the immune system. Thus, cyclic di-nucleotides are also potential immunotherapeutic agents to treat antibiotic-resistant bacterial infections...
Cyclic di-GMP, an established secondary messenger still speeding upUte Romling
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Environ Microbiol 14:1817-29. 2012....
Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopyKristina Jonas
Swedish Institute for Infectious Disease Control, Solna, Sweden
BMC Microbiol 7:70. 2007....
Prevailing concepts of c-di-GMP signalingUte Romling
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Contrib Microbiol 16:161-81. 2009..However, the exact molecular mechanisms of c-di-GMP action remain to be discovered...
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and ArchaeaUte Romling
Karolinska Institutet, Department of Microbiology, Tumor and Cell Biology, FE280, SE 171 77 Stockholm, Sweden
Sci Signal 1:pe39. 2008..To elucidate the biological importance and molecular mechanisms of action for c-di-AMP and the recently recognized second messenger c-di-GMP will require a multidisciplinary approach...
Cyclic di-GMP as a second messengerUte Romling
Karolinska Institutet, Microbiology and Tumor Biology Center MTC, Box 280, SE 171 77 Stockholm, Sweden
Curr Opin Microbiol 9:218-28. 2006..Besides its role as an intracellular signaling molecule in bacteria, c-di-GMP also acts as an intercellular signaling molecule between prokaryotes and also has effects in eukaryotes that could provide a perspective in cancer treatment...
Complex regulatory network encompassing the Csr, c-di-GMP and motility systems of Salmonella TyphimuriumKristina Jonas
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE 17177 Stockholm, Sweden
Environ Microbiol 12:524-40. 2010..Altogether, our data reveal that the concerted and complex regulation of several genes encoding GGDEF/EAL domain proteins allows CsrA to control the motility-sessility switch in S. Typhimurium at multiple levels...
Unphosphorylated CsgD controls biofilm formation in Salmonella enterica serovar TyphimuriumKatherina Zakikhany
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, FE 280, 17177 Stockholm, Sweden
Mol Microbiol 77:771-86. 2010..In summary, our findings suggest that the response regulator CsgD functions in its unphosphorylated form under the conditions of biofilm formation investigated in this study...
GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motilityRoger Simm
Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
Mol Microbiol 53:1123-34. 2004..Thus, the data suggest that c-di-GMP is a novel global second messenger in bacteria the metabolism of which is controlled by GGDEF and EAL domain proteins...
Characterization of cellulose production in Escherichia coli Nissle 1917 and its biological consequencesClaudia Monteiro
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
Environ Microbiol 11:1105-16. 2009..The role of cellulose in this strain is in contrast to the role of cellulose in the commensal strain E. coli TOB1. Consequently, the role of cellulose in bacterial-host interaction is dependent on the E. coli strain background...
Cyclic di-GMP signalling controls virulence properties of Salmonella enterica serovar Typhimurium at the mucosal liningAgaristi Lamprokostopoulou
Department of Microbiology, Tumor and Cell Biology, FE number 280, Karolinska Institutet, 17177 Stockholm, Sweden
Environ Microbiol 12:40-53. 2010..Our results suggest that transition between biofilm formation and virulence in S. typhimurium at the epithelial cell lining is mediated by c-di-GMP signalling through CsgD and cellulose expression...
The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteinsKristina Jonas
Swedish Institute for Infectious Disease Control, SE 17182, Solna and Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, SE 17177 Stockholm, Sweden
Mol Microbiol 70:236-57. 2008..All together, these data demonstrate a global role for CsrA in the regulation of c-di-GMP metabolism by regulating the expression of GGDEF proteins at the post-transcriptional level...
Complex c-di-GMP signaling networks mediate transition between virulence properties and biofilm formation in Salmonella enterica serovar TyphimuriumIrfan Ahmad
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
PLoS ONE 6:e28351. 2011..Our findings show that c-di-GMP signaling is at least equally important in the regulation of Salmonella-host interaction as in the regulation of biofilm formation at ambient temperature...
Characteristics of biofilms from urinary tract catheters and presence of biofilm-related components in Escherichia coliXiaoda Wang
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, FE 280, SE 171 77, Stockholm, Sweden
Curr Microbiol 60:446-53. 2010....
Hfq and Hfq-dependent small RNAs are major contributors to multicellular development in Salmonella enterica serovar TyphimuriumClaudia Monteiro
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
RNA Biol 9:489-502. 2012..curli fimbriae surface-attachment at ambient temperature. Consequently, ArcZ is a major regulator of rdar biofilm development...
A role for the EAL-like protein STM1344 in regulation of CsgD expression and motility in Salmonella enterica serovar TyphimuriumRoger Simm
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
J Bacteriol 191:3928-37. 2009..Consequently, although STM1344 has not retained a direct role in c-di-GMP metabolism, it still participates in the regulation of c-di-GMP turnover and has a role in the transition between sessility and motility...
Bistable expression of CsgD in biofilm development of Salmonella enterica serovar typhimuriumNina Grantcharova
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Nobels vag 16, Stockholm, Sweden
J Bacteriol 192:456-66. 2010..Consequently, bistability at the level of CsgD expression leads to a corresponding pattern of task distribution in S. Typhimurium biofilms...
Regulatory components at the csgD promoter--additional roles for OmpR and integration host factor and role of the 5' untranslated regionUlrich Gerstel
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Stockholm, Sweden
FEMS Microbiol Lett 261:109-17. 2006..Furthermore, the role of the 174 bp long 5'-untranslated region on transcriptional activity was defined...
Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometryRoger Simm
Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
Anal Biochem 386:53-8. 2009..We describe the use of the methodology to assess the change in c-di-GMP concentration during the growth phase and the contribution of a point mutation in S. typhimurium to the overall cellular c-di-GMP concentration...
The role of c-di-GMP signaling in an Aeromonas veronii biovar sobria strainMokhlasur Rahman
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
FEMS Microbiol Lett 273:172-9. 2007..On the other hand, overexpression of the EAL domain protein YhjH decreased biofilm formation and increased motility...
Role of EAL-containing proteins in multicellular behavior of Salmonella enterica serovar TyphimuriumRoger Simm
Department of Microbiology, Tumor and Cell Biology MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
J Bacteriol 189:3613-23. 2007..Consequently, this work has shown that several EAL and GGDEF-EAL domain proteins, which act as phosphodiesterases, play a determinative role in the expression level of multicellular behavior of Salmonella enterica serovar Typhimurium...
Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37Ylva Kai-Larsen
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
PLoS Pathog 6:e1001010. 2010..Taken together, our data demonstrate that biofilm components are involved in the pathogenesis of urinary tract infections by E. coli and can be a target of local immune defense mechanisms...
C-di-GMP: the dawning of a novel bacterial signalling systemUte Romling
Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, SE 17177 Stockholm, Sweden
Mol Microbiol 57:629-39. 2005..The accumulated data clearly indicate that a novel ubiquitous signalling system in bacteria has been discovered...
Regulation of c-di-GMP metabolism in biofilmsKristina Jonas
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE 17177 Stockholm, Sweden
Future Microbiol 4:341-58. 2009....
Regulation of biofilm components in Salmonella enterica serovar Typhimurium by lytic transglycosylases involved in cell wall turnoverClaudia Monteiro
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, FE28017177, Stockholm, Sweden
J Bacteriol 193:6443-51. 2011..These findings reveal, for the first time, a specific function of lytic transglycosylases in S. Typhimurium and suggest the existence of a new signaling pathway that links cell wall turnover to biofilm formation...
Opposing contributions of polynucleotide phosphorylase and the membrane protein NlpI to biofilm formation by Salmonella enterica serovar TyphimuriumSyed Fazle Rouf
Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
J Bacteriol 193:580-2. 2011..Polynucleotide phosphorylase and NlpI also had opposite effects on the expression of yjcC, which codes for a cyclic-3',5'-di-guanylate phosphodiesterase affecting CsgD expression...
Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environmentDinesh Diraviam Sriramulu
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
Proteomics 5:3712-21. 2005..Altogether, our results elucidate adaptive strategies of P. aeruginosa towards the nutrient-rich CF lung habitat during the course of chronic colonization...
Worldwide distribution of Pseudomonas aeruginosa clone C strains in the aquatic environment and cystic fibrosis patientsUte Romling
Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Stockholm, Sweden
Environ Microbiol 7:1029-38. 2005..Elucidation of the mechanisms which lead to the prevalence of clone C strain in patients and the environment requires the investigation of additional phenotypes...
Occurrence and regulation of the multicellular morphotype in Salmonella serovars important in human diseaseUte Romling
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden
Int J Med Microbiol 293:273-85. 2003..Therefore, the expression pattern of the rdar morphotype is serovar specific and correlates with a disease phenotype breaching the intestinal epithelial cell lining...
Complex regulation of csgD promoter activity by global regulatory proteinsUlrich Gerstel
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden
Mol Microbiol 49:639-54. 2003..A complex regulatory network involving IHF, H-NS and OmpR is proposed whereby the nucleo-complex composition in the csgD-csgBA intergenic region is altered in response to oxygen tension...
