Research Topics
Genomes and Genes | Olof RadmarkSummaryAffiliation: Karolinska Institutet Country: Sweden Publications
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Publications
Zymosan suppresses leukotriene C₄ synthase activity in differentiating monocytes: antagonism by aspirin and protein kinase inhibitorsJulia Esser
Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
FASEB J 25:1417-27. 2011..Our results suggest a late PGE(2)-mediated phosphoregulation of LTC(4)S during microbial exposure, which may contribute to resolution of inflammation, with implications for aspirin hypersensitivity...- Regulation of the activity of 5-lipoxygenase, a key enzyme in leukotriene biosynthesisOlof Radmark
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, 17177 Stockholm, Sweden
Biochem Biophys Res Commun 396:105-10. 2010..Also, possible effects of 5LO products in relation to tumorigenesis have been described. Thus, insight regarding the biochemistry of 5LO is relevant for better understanding of normal physiology, and for development of therapy... 
5-Lipoxygenase: regulation of expression and enzyme activityOlof Radmark
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 17177 Stockholm, Sweden
Trends Biochem Sci 32:332-41. 2007..With regard to the control of enzyme activity, many of these findings focus on the N-terminal domain of 5-LO...- 5-lipoxygenase: regulation and possible involvement in atherosclerosisOlof Radmark
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, S 17177 Stockholm, Sweden
Prostaglandins Other Lipid Mediat 83:162-74. 2007..This review article focuses on two aspects regarding 5-lipoxygenase. First, mechanisms for activation of the enzyme. Second, the involvement of 5-lipoxygenase and leukotrienes in atherosclerosis... - Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102Julia Esser
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
Biochem J 425:265-74. 2010..The results of the present study support a role for CLP as a chaperoning scaffold factor, influencing both the stability and the activity of 5-LO... 
Coactosin-like protein supports 5-lipoxygenase enzyme activity and up-regulates leukotriene A4 productionMarija Rakonjac
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
Proc Natl Acad Sci U S A 103:13150-5. 2006..Furthermore, in stimulated cells, CLP appears to function in a complex together with 5LO and membranes, increasing the capacity of 5LO for leukotriene biosynthesis...- Human Dicer C-terminus functions as a 5-lipoxygenase binding domainVildan Dincbas-Renqvist
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
Biochim Biophys Acta 1789:99-108. 2009..Providing a link between miRNA-mediated regulation of gene expression and inflammation, our results suggest that the formation of miRNAs may be regulated by 5LO in leukocytes and cancer cells expressing this lipoxygenase... - Arachidonate 5-lipoxygenaseOlof Radmark
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
Prostaglandins Other Lipid Mediat 68:211-34. 2002..Also, studies on phosphorylation of 5-lipoxygenase, interaction with other proteins, and the intracellullar mobility of 5-lipoxygenase, are reviewed... - GC-rich sequences in the 5-lipoxygenase gene promoter are required for expression in Mono Mac 6 cells, characterization of a novel Sp1 binding siteDavid Dishart
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II Karolinska Institutet, S 171 77 Stockholm, Sweden
Biochim Biophys Acta 1738:37-47. 2005..Thus, the GC-rich part of the 5LO gene promoter, including a novel Sp1 site, appear important for basal (rather than upregulated) transcription of 5LO in MM6 cells... - Cloning of rat p47phox and comparison with human p47phoxMichiharu Tanabe
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77, Stockholm, Sweden
DNA Seq 16:65-8. 2005..Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox... - Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2)Oliver Werz
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
J Biol Chem 277:14793-800. 2002.... - Mutation analysis of the human 5-lipoxygenase C-terminus: support for a stabilizing C-terminal loopHisayo Okamoto
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
Biochim Biophys Acta 1749:123-31. 2005..In the structure of soybean lipoxygenase-1, a C-terminal loop was pointed out as important for correct orientation of the C-terminus. Accordingly, a hydrogen bond appears to stabilize such a C-terminal loop also in 5LO... - Exosomes from human macrophages and dendritic cells contain enzymes for leukotriene biosynthesis and promote granulocyte migrationJulia Esser
Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
J Allergy Clin Immunol 126:1032-40, 1040.e1-4. 2010..Recently, nanovesicles (exosomes), released from macrophages and dendritic cells (DCs), have become increasingly appreciated as messengers in immunity... - Ribonuclease activity and RNA binding of recombinant human DicerPatrick Provost
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
EMBO J 21:5864-74. 2002..Availability of the recombinant Dicer protein will help improve our understanding of RNA silencing and other Dicer-related processes... - 5-Lipoxygenase: mechanisms of regulationOlof Radmark
Department Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 17177 Stockholm, Sweden
J Lipid Res 50:S40-5. 2009..Furthermore, possible effects of 5-LO metabolites in relation to tumorigenesis have emerged. Thus, an understanding of the biochemistry of this lipoxygenase has potential implications for treatment of various diseases... - Dicer is required for chromosome segregation and gene silencing in fission yeast cellsPatrick Provost
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, S 171 77 Stockholm, Sweden Europe
Proc Natl Acad Sci U S A 99:16648-53. 2002..Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif... - Expression of 5-lipoxygenase and 15-lipoxygenase in rheumatoid arthritis synovium and effects of intraarticular glucocorticoidsKarina Roxana Gheorghe
Department of Biosciences and Nutrition, Novum, Karolinska Institute, SE 141 57 Huddinge, Sweden
Arthritis Res Ther 11:R83. 2009..We therefore aimed to investigate the 5-LO and 15-LO-1 expression pattern in RA and ostheoarthritis (OA) synovial tissue and to study the effect of intraarticular glucocorticoid (GC) therapy on enzyme expression... - C-terminus of p47(phox) is required for interaction with leukocyte type rat 12-lipoxygenaseMichiharu Tanabe
Department of Medical Biochemistry and Biophysics, Karolinska Instituet, Stockholm, Sweden
Protein Pept Lett 12:295-7. 2005..These results indicate that C-terminus of p47(phox) is important for its interaction to rat 12-LO... - Microsomal prostaglandin E synthase 1 determines tumor growth in vivo of prostate and lung cancer cellsHiromi Hanaka
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
Proc Natl Acad Sci U S A 106:18757-62. 2009..The results suggest that MPGES-1 is an alternative therapeutic target in cancer cells expressing this enzyme... - Regulation of 5-lipoxygenase enzyme activityOlof Radmark
Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 17177 Stockholm, Sweden
Biochem Biophys Res Commun 338:102-10. 2005..This is followed by the activating factors: Ca2+, membranes, ATP, and lipid hydroperoxide. Also, studies on phosphorylation of 5-lipoxygenase and nuclear localization sequences are reviewed... - Molecular cloning and functional characterization of mouse coactosin-like proteinJohanne Doucet
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, S 171 77, Sweden
Biochem Biophys Res Commun 290:783-9. 2002..However, association with actin was best observed at low salt concentrations, while association with 5LO was favored by salt, indicating different binding characteristics... - Calcium binding to 5-lipoxygenaseTove Hammarberg
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden
Adv Exp Med Biol 507:117-21. 2002 - Activated LTB4 pathway in muscle tissue of patients with polymyositis or dermatomyositisIngela Loell
Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, Stockholm SE 171 76, Sweden
Ann Rheum Dis 72:293-9. 2013..To investigate the involvement of the leukotriene B4 (LTB4) pathway in polymyositis (PM) and dermatomyositis (DM) and the effect of immunosuppressive treatment on the LTB4 pathway... - Thr94 in bovine myelin basic protein is a second phosphorylation site for 42-kDa mitogen-activated protein kinase (ERK2)Daniel Hirschberg
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
J Protein Chem 22:177-81. 2003..The results provide information for delineation of a further substrate consensus motif for ERK2 phosphorylation... - Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic beta-cellsOrison O Woolcott
Karolinska Institutet, Sodersjukhuset, Department of Internal Medicine, Research Center, SE 118 83 Stockholm, Sweden
Cell Calcium 39:529-37. 2006..These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in beta-cells...