U C Oppermann

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi Function, gene organization and protein structures of 11beta-hydroxysteroid dehydrogenase isoforms
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 249:355-60. 1997
  2. ncbi Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity
    Stanley S Ng
    Structural Genomics Consortium, Botnar Research Center, University of Oxford, Oxford OX3 7LD, UK
    Nature 448:87-91. 2007
  3. ncbi Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus. Co-purification of RNase A with Sso7c
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    FEBS Lett 432:141-4. 1998
  4. ncbi Carbonyl reduction of an anti-insect agent imidazole analogue of metyrapone in soil bacteria, invertebrate and vertebrate species
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Chem Biol Interact 114:211-24. 1998
  5. ncbi Binding of amyloid beta-peptide to mitochondrial hydroxyacyl-CoA dehydrogenase (ERAB): regulation of an SDR enzyme activity with implications for apoptosis in Alzheimer's disease
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 451:238-42. 1999
  6. ncbi Short-chain dehydrogenases/reductases (SDR): the 2002 update
    Udo Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177, Stockholm, Sweden
    Chem Biol Interact 143:247-53. 2003
  7. ncbi Heterogeneity of 11beta-hydroxysteroid dehydrogenase type 1/microsomal carbonyl reductase (11beta-HSD/CR) in guinea pig tissues. Purification of the liver form suggests modification in the cosubstrate binding site
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Toxicology 144:63-9. 2000
  8. ncbi Molecular and structural aspects of xenobiotic carbonyl metabolizing enzymes. Role of reductases and dehydrogenases in xenobiotic phase I reactions
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Toxicology 144:71-81. 2000
  9. ncbi Forms and functions of human SDR enzymes
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Chem Biol Interact 130:699-705. 2001
  10. ncbi Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids
    H U Marschall
    Karolinska Institutet, Division of Gastroenterology and Hepatology, Huddinge University Hospital, SE 141 86 Huddinge, Sweden
    Hepatology 31:990-6. 2000

Collaborators

Detail Information

Publications45

  1. ncbi Function, gene organization and protein structures of 11beta-hydroxysteroid dehydrogenase isoforms
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 249:355-60. 1997
    ..On the other hand, for adrenal glucocorticoids the type-2 enzyme seems to be exclusively a dehydrogenase and, by inactivating glucocorticoids, confers specificity to peripheral mineralocorticoid receptors...
  2. ncbi Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity
    Stanley S Ng
    Structural Genomics Consortium, Botnar Research Center, University of Oxford, Oxford OX3 7LD, UK
    Nature 448:87-91. 2007
    ....
  3. ncbi Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus. Co-purification of RNase A with Sso7c
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    FEBS Lett 432:141-4. 1998
    ....
  4. ncbi Carbonyl reduction of an anti-insect agent imidazole analogue of metyrapone in soil bacteria, invertebrate and vertebrate species
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Chem Biol Interact 114:211-24. 1998
    ..These findings suggest that the high CR activities found in these fractions belong to different subgroups of SDR or AKR...
  5. ncbi Binding of amyloid beta-peptide to mitochondrial hydroxyacyl-CoA dehydrogenase (ERAB): regulation of an SDR enzyme activity with implications for apoptosis in Alzheimer's disease
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 451:238-42. 1999
    ..The interaction of A beta with ERAB further links oxidoreductase activity with both apoptosis and amyloid toxicity...
  6. ncbi Short-chain dehydrogenases/reductases (SDR): the 2002 update
    Udo Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177, Stockholm, Sweden
    Chem Biol Interact 143:247-53. 2003
    ....
  7. ncbi Heterogeneity of 11beta-hydroxysteroid dehydrogenase type 1/microsomal carbonyl reductase (11beta-HSD/CR) in guinea pig tissues. Purification of the liver form suggests modification in the cosubstrate binding site
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Toxicology 144:63-9. 2000
    ..Both have an apparent molecular mass of 32 kDa, suggesting protein modifications occurring in the type 1 isozyme which account for the differences in chromatographic behaviour...
  8. ncbi Molecular and structural aspects of xenobiotic carbonyl metabolizing enzymes. Role of reductases and dehydrogenases in xenobiotic phase I reactions
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Toxicology 144:71-81. 2000
    ..A wealth of biochemical, genetic and structural data now establishes these families to constitute important phase I enzymes...
  9. ncbi Forms and functions of human SDR enzymes
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Chem Biol Interact 130:699-705. 2001
    ..This overview, prepared just before completion of the human genome project, gives the different human SDR forms and relates them to human diseases...
  10. ncbi Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids
    H U Marschall
    Karolinska Institutet, Division of Gastroenterology and Hepatology, Huddinge University Hospital, SE 141 86 Huddinge, Sweden
    Hepatology 31:990-6. 2000
    ..Hydroxysteroid dehydrogenases are candidates for the binding and transport of 3alpha-hydroxy bile acids. We assume that ADH gammagamma isozyme is involved in cytosolic bile acid binding and transport processes as well...
  11. ncbi Novel enzymological profiles of human 11beta-hydroxysteroid dehydrogenase type 1
    M Hult
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Chem Biol Interact 130:805-14. 2001
    ..In conclusion, evidence is provided that human 11beta-HSD1 in vitro is involved in phase I reactions of anti-inflammatory non-steroidal drugs like ketoprofen and DFU-lactol...
  12. ncbi Human type 10 17 beta-hydroxysteroid dehydrogenase: molecular modelling and substrate docking
    E Nordling
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden
    J Mol Graph Model 19:514-20, 591-3. 2001
    ..The model is suitable to explore substrate and inhibitor characteristics that may be used in the development of novel strategies in the treatment of degenerative or malignant diseases...
  13. ncbi High-level expression and rapid purification of rare-codon genes from hyperthermophilic archaea by the GST gene fusion system
    Xiaoqiu Wu
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    J Chromatogr B Analyt Technol Biomed Life Sci 786:177-85. 2003
    ..The affinity chromatography purification followed by a heating step is an efficient and convenient method for thermostable protein purification...
  14. ncbi An ethanol-inducible MDR ethanol dehydrogenase/acetaldehyde reductase in Escherichia coli: structural and enzymatic relationships to the eukaryotic protein forms
    J Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 263:305-11. 1999
    ..Although present in several strains under different conditions, inducibility may constitute an explanation for the fairly late characterization of this E. coli gene product...
  15. ncbi Critical residues for structure and catalysis in short-chain dehydrogenases/reductases
    Charlotta Filling
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 277:25677-84. 2002
    ..This extends the previously recognized catalytic triad of Ser-Tyr-Lys residues to form a tetrad of Asn-Ser-Tyr-Lys in the majority of characterized short-chain dehydrogenases/reductase enzymes...
  16. ncbi Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance
    Xiaoqiu Wu
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE 171 77, Sweden
    Biochem Biophys Res Commun 313:89-96. 2004
    ..Taken together, the results underscore the importance of mRNA levels and RNA stability, but not necessarily tRNA abundance for efficient heterologous protein production in E. coli...
  17. doi Superfamilies SDR and MDR: from early ancestry to present forms. Emergence of three lines, a Zn-metalloenzyme, and distinct variabilities
    Hans Jornvall
    Department of Medical Biochemistry and Biophysics MBB, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Biochem Biophys Res Commun 396:125-30. 2010
    ..Differences among structural and catalytic zinc ions may be relative and involve several states. Combined, the comparisons trace evolutionary properties of huge superfamilies, with partially redundant enzymes in cellular redox functions...
  18. ncbi Biochemical characterization of TASSELSEED 2, an essential plant short-chain dehydrogenase/reductase with broad spectrum activities
    Xiaoqiu Wu
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS J 274:1172-82. 2007
    ....
  19. pmc Protein production and purification
    Susanne Gräslund
    Karolinska Institutet, Scheeles vag 2, 171 77 Stockholm, Sweden
    Nat Methods 5:135-46. 2008
    ..This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators...
  20. ncbi Short-chain dehydrogenases/reductases (SDRs)
    Yvonne Kallberg
    Department of Medical Biochemistry and Biophysics and Stockholm Bioinformatics Centre, Karolinska Institutet, Sweden
    Eur J Biochem 269:4409-17. 2002
    ..We show that, in spite of the great diversity of the SDR superfamily, the primary structure alone can be used for functional assignments and for predictions of coenzyme preference...
  21. ncbi Liver X receptors downregulate 11beta-hydroxysteroid dehydrogenase type 1 expression and activity
    Thomas M Stulnig
    Department of Medical Nutrition and Biosciences, Karolinska Institutet, Huddinge, Sweden
    Diabetes 51:2426-33. 2002
    ..In conclusion, LXR ligands could mediate beneficial metabolic effects in insulin resistance syndromes including type 2 diabetes by interfering with peripheral glucocorticoid activation...
  22. pmc Short-chain dehydrogenase/reductase (SDR) relationships: a large family with eight clusters common to human, animal, and plant genomes
    Yvonne Kallberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Protein Sci 11:636-41. 2002
    ..Close to half of these gene products represent steroid dehydrogenases, emphasizing the regulatory importance of these enzymes...
  23. ncbi Active site variability of type 1 11beta-hydroxysteroid dehydrogenase revealed by selective inhibitors and cross-species comparisons
    Malin Hult
    Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Mol Cell Endocrinol 248:26-33. 2006
    ..Taken together, this structure-activity study provides increased insight into active site complexity and catalytic mechanism of 11beta-HSD1, useful for further inhibitor design...
  24. pmc Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activity
    Petra Lukacik
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, UK
    Biochem J 402:419-27. 2007
    ..It also reveals a broad and deep active site cleft into which NAD+ and oestradiol can be docked in a catalytically competent orientation...
  25. pmc Mechanism and substrate recognition of human holo ACP synthase
    Gabor Bunkoczi
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, UK
    Chem Biol 14:1243-53. 2007
    ..A detailed mechanism is proposed describing the substrate binding and catalytic process...
  26. ncbi Reversible sequestration of active site cysteines in a 2Fe-2S-bridged dimer provides a mechanism for glutaredoxin 2 regulation in human mitochondria
    Catrine Johansson
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford OX3 7LD, United Kingdom
    J Biol Chem 282:3077-82. 2007
    ..Under oxidizing conditions, the dimers would readily separate into iron-free active monomers, providing a structural explanation for glutaredoxin activation under oxidative stress...
  27. pmc The molecular mechanism of nitrogen-containing bisphosphonates as antiosteoporosis drugs
    Kathryn L Kavanagh
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, United Kingdom
    Proc Natl Acad Sci U S A 103:7829-34. 2006
    ..These experiments reveal the molecular binding characteristics of an important pharmacological target and provide a route for further optimization of these important drugs...
  28. pmc Expanded substrate screenings of human and Drosophila type 10 17beta-hydroxysteroid dehydrogenases (HSDs) reveal multiple specificities in bile acid and steroid hormone metabolism: characterization of multifunctional 3alpha/7alpha/7beta/17beta/20beta/21-H
    Naeem Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem J 376:49-60. 2003
    ....
  29. ncbi Molecular mechanisms underlying WOX1 activation during apoptotic and stress responses
    Nan Shan Chang
    Laboratory of Molecular Immunology, Guthrie Research Institute, 1 Guthrie Square, Sayre, PA 18840, USA
    Biochem Pharmacol 66:1347-54. 2003
    ..Taken together, WOX1 is involved in stress and apoptotic responses, and is likely to regulate the activation of both p53 and JNK1...
  30. ncbi Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase
    Kunde Guo
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, United Kingdom
    J Biol Chem 281:10291-7. 2006
    ..The properties determined for DHRS6 suggest a possible physiological role in cytosolic ketone body utilization, either as a secondary system for energy supply in starvation or to generate precursors for lipid and sterol synthesis...
  31. ncbi Tic32, an essential component in chloroplast biogenesis
    Friederike Hörmann
    Department of Biology and Plant Biochemistry, University of Munich, Menzinger Strasse 67, 80638 Munich, Germany
    J Biol Chem 279:34756-62. 2004
    ..The homology of Tic32 to short-chain dehydrogenases suggests a dual role of Tic32 in import, one as a regulatory component and one as an important subunit in the assembly of the entire complex...
  32. ncbi Coenzyme-based functional assignments of short-chain dehydrogenases/reductases (SDRs)
    Bengt Persson
    IFM Bioinformatics, Linkoping University, S 581 83, Linkoping, Sweden
    Chem Biol Interact 143:271-8. 2003
    ..We also find that NADP(H) is the preferred coenzyme among most classical SDRs, while NAD(H) is that preferred among most extended SDRs...
  33. doi Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study
    Nicola A Burgess-Brown
    The Structural Genomics Consortium, Old Road Campus Research Building, University of Oxford, Roosevelt Drive, Oxford OX3 7DQ, UK
    Protein Expr Purif 59:94-102. 2008
    ..The trend is that heterologous expression of some proteins in bacteria can be improved by altering codon preference, but that this effect can be generally recapitulated by introducing rare codon tRNAs into the host cell...
  34. doi Ligand supplementation as a method to increase soluble heterologous protein production
    Viktorija Hozjan
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford OX3 7LD, UK
    Expert Rev Proteomics 5:137-43. 2008
    ....
  35. ncbi Hot spots in Tcf4 for the interaction with beta-catenin
    Marina Fasolini
    Pharmacia Corporation Discovery Research Oncology, Department of Chemistry, Viale Pasteur 10, 20014 Nerviano, Italy
    J Biol Chem 278:21092-8. 2003
    ..The measured thermodynamic data are discussed with the available structural information of Tcf-beta-catenin crystal structures and allow us to propose possible sites for development of Tcf antagonists...
  36. ncbi Evaluation of micro-parallel liquid chromatography as a method for HTS-coupled actives verification
    Anton Simeonov
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892 3370, USA
    Assay Drug Dev Technol 5:815-24. 2007
    ..We discuss the benefits of microPLC and its limitations from the standpoint of ease of use and integration into a seamless postscreen workflow...
  37. ncbi SDR-type human hydroxysteroid dehydrogenases involved in steroid hormone activation
    Xiaoqiu Wu
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, United Kingdom
    Mol Cell Endocrinol 265:71-6. 2007
    ..Several isoforms constitute promising drug targets, of particular importance in cancer, metabolic diseases, neurodegeneration and immunity...
  38. ncbi High-level production and optimization of monodispersity of 11beta-hydroxysteroid dehydrogenase type 1
    Björn Elleby
    Department of Assay Development and Screening, Biovitrum AB, Stockholm SE 112 76, Sweden
    Biochim Biophys Acta 1700:199-207. 2004
    ..The outlined procedure may provide a general method for preparing similar proteins to oligomeric homogeneity and with retained biological activity...
  39. ncbi Type 1 11beta-hydroxysteroid dehydrogenase as universal drug target in metabolic diseases?
    Udo Oppermann
    Structural Genomics Consortium, University of Oxford, Oxford, OX3 7LD, UK
    Endocr Metab Immune Disord Drug Targets 6:259-69. 2006
    ..Taken together, it appears that inhibitors against 11beta-HSD1 constitute a promising avenue for novel treatment strategies against the underlying causes of cardiovascular and other metabolic diseases...
  40. ncbi Carbonyl reductases: the complex relationships of mammalian carbonyl- and quinone-reducing enzymes and their role in physiology
    Udo Oppermann
    Structural Genomics Consortium, Botnar Research Center, University of Oxford, Oxford, OX3 7LD, United Kingdom
    Annu Rev Pharmacol Toxicol 47:293-322. 2007
    ..This review summarizes the current knowledge on structure and function relationships of the major human and mammalian carbonyl reductases identified...
  41. ncbi The crystal structure of human geranylgeranyl pyrophosphate synthase reveals a novel hexameric arrangement and inhibitory product binding
    Kathryn L Kavanagh
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, United Kingdom
    J Biol Chem 281:22004-12. 2006
    ..Structural comparisons between members of this enzyme class give deeper insights into conserved features important for catalysis...
  42. ncbi The crystal structure of guinea pig 11beta-hydroxysteroid dehydrogenase type 1 provides a model for enzyme-lipid bilayer interactions
    Derek Ogg
    Department of Structural Chemistry, Biovitrum, SE 112 76 Stockholm, Sweden
    J Biol Chem 280:3789-94. 2005
    ..The structure provides a model for enzyme-lipid bilayer interactions and suggests a funneling of lipophilic substrates such as steroid hormones from the hydrophobic membrane environment to the enzyme active site...
  43. ncbi Structure of human phytanoyl-CoA 2-hydroxylase identifies molecular mechanisms of Refsum disease
    Michael A McDonough
    Oxford Centre for Molecular Sciences and Department of Chemistry, University of Oxford, Mansfield Road, Oxford OX1 3TA, United Kingdom
    J Biol Chem 280:41101-10. 2005
    ..PAHX may be the first of a new subfamily of coenzyme A-binding 2OG oxygenases...
  44. ncbi Structure and function of human 17beta-hydroxysteroid dehydrogenases
    Petra Lukacik
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, United Kingdom
    Mol Cell Endocrinol 248:61-71. 2006
    ..Several 17beta-HSDs enzymes constitute promising drug targets, of particular importance in cancer, metabolic diseases, neurodegeneration and possibly immunity...
  45. ncbi Comparative enzymology of 11 beta -hydroxysteroid dehydrogenase type 1 from glucocorticoid resistant (Guinea pig) versus sensitive (human) species
    Naeem Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 278:2030-5. 2003
    ..Instead, the expression of 11 beta-hydroxysteroid dehydrogenase type 1 in the Zona glomerulosa of the guinea pig adrenal gland suggests a role of this enzyme in mineralocorticoid synthesis in this hypercortisolic species...