Rolf Ohlsson

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi request reprint Chromosomal networks as mediators of epigenetic states: the maternal genome connection
    Noriyuki Sumida
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    Epigenetics 5:297-300. 2010
  2. pmc Transforming growth factor beta promotes complexes between Smad proteins and the CCCTC-binding factor on the H19 imprinting control region chromatin
    Rosita Bergstrom
    Ludwig Institute for Cancer Research, Uppsala University, SE 751 24 Uppsala, Sweden
    J Biol Chem 285:19727-37. 2010
  3. doi request reprint Does CTCF mediate between nuclear organization and gene expression?
    Rolf Ohlsson
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    Bioessays 32:37-50. 2010
  4. pmc CTCF shapes chromatin by multiple mechanisms: the impact of 20 years of CTCF research on understanding the workings of chromatin
    Rolf Ohlsson
    Institute for Microbiology, Tumor and Cellbiology, Karolinska Institute, Stockholm, Sweden
    Chromosoma 119:351-60. 2010
  5. ncbi request reprint Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions
    Zhihu Zhao
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, SE 752 36 Uppsala, Sweden
    Nat Genet 38:1341-7. 2006
  6. pmc Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritance
    Vinod Pant
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Mol Cell Biol 24:3497-504. 2004
  7. ncbi request reprint Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation
    Wenqiang Yu
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Nat Genet 36:1105-10. 2004
  8. pmc CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2
    Sreenivasulu Kurukuti
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Proc Natl Acad Sci U S A 103:10684-9. 2006
  9. doi request reprint CTCF-binding sites within the H19 ICR differentially regulate local chromatin structures and cis-acting functions
    Sylvain Guibert
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Uppsala, Sweden
    Epigenetics 7:361-9. 2012
  10. pmc An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region
    Noopur Thakur
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Mol Cell Biol 24:7855-62. 2004

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Chromosomal networks as mediators of epigenetic states: the maternal genome connection
    Noriyuki Sumida
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    Epigenetics 5:297-300. 2010
    ..We also discuss the possibility that the genome is physically linked by means of maternal-specific epigenetic marks during development...
  2. pmc Transforming growth factor beta promotes complexes between Smad proteins and the CCCTC-binding factor on the H19 imprinting control region chromatin
    Rosita Bergstrom
    Ludwig Institute for Cancer Research, Uppsala University, SE 751 24 Uppsala, Sweden
    J Biol Chem 285:19727-37. 2010
    ..Because the CTCF-Smad complex is not essential for the chromatin insulator function of the H19 ICR, we propose that it can play a role in chromatin cross-talk organized by the H19 ICR...
  3. doi request reprint Does CTCF mediate between nuclear organization and gene expression?
    Rolf Ohlsson
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    Bioessays 32:37-50. 2010
    ....
  4. pmc CTCF shapes chromatin by multiple mechanisms: the impact of 20 years of CTCF research on understanding the workings of chromatin
    Rolf Ohlsson
    Institute for Microbiology, Tumor and Cellbiology, Karolinska Institute, Stockholm, Sweden
    Chromosoma 119:351-60. 2010
    ..Two decades of research has advanced the understanding of the fundamental role that CTCF plays in regulating such a vast expanse of DNA...
  5. ncbi request reprint Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions
    Zhihu Zhao
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, SE 752 36 Uppsala, Sweden
    Nat Genet 38:1341-7. 2006
    ..These observations shed new light on development, cancer epigenetics and the evolution of imprinting...
  6. pmc Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritance
    Vinod Pant
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Mol Cell Biol 24:3497-504. 2004
    ..Considering many other CTCF targets, we propose that site-specific interactions between various DNA-bound CTCF molecules may provide general focal points in the organization of looped chromatin domains involved in gene regulation...
  7. ncbi request reprint Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation
    Wenqiang Yu
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Nat Genet 36:1105-10. 2004
    ..We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions...
  8. pmc CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2
    Sreenivasulu Kurukuti
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Proc Natl Acad Sci U S A 103:10684-9. 2006
    ....
  9. doi request reprint CTCF-binding sites within the H19 ICR differentially regulate local chromatin structures and cis-acting functions
    Sylvain Guibert
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Uppsala, Sweden
    Epigenetics 7:361-9. 2012
    ....
  10. pmc An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region
    Noopur Thakur
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Mol Cell Biol 24:7855-62. 2004
    ..Double-stranded RNA (dsRNA)-mediated RNA interference is unlikely to be involved, as the ICR is active irrespective of the simultaneous production of dsRNA from the genes it silences...
  11. pmc The nucleotides responsible for the direct physical contact between the chromatin insulator protein CTCF and the H19 imprinting control region manifest parent of origin-specific long-distance insulation and methylation-free domains
    Vinod Pant
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Genes Dev 17:586-90. 2003
    ..Because CTCF interacts with other imprinting control regions, it emerges as a central factor responsible for interpreting and propagating gamete-derived epigenetic marks and for organizing epigenetically controlled expression domains...
  12. ncbi request reprint CTCF regulates asynchronous replication of the imprinted H19/Igf2 domain
    Rosita Bergstrom
    Department of Development and Genetics, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden
    Cell Cycle 6:450-4. 2007
    ..Here we show that maternal, but not paternal inheritance of a mutated H19 imprinting control region, lacking functional CTCF binding sites, underlies a late to early switch in replication timing of the maternal H19/Igf2 domain...
  13. ncbi request reprint CpG methylation of the IFNG gene as a mechanism to induce immunosuppression [correction of immunosupression] in tumor-infiltrating lymphocytes
    Peter C J Janson
    Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden
    J Immunol 181:2878-86. 2008
    ....
  14. pmc Nonallelic transvection of multiple imprinted loci is organized by the H19 imprinting control region during germline development
    Kuljeet Singh Sandhu
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Genes Dev 23:2598-603. 2009
    ..We conclude that one locus can directly or indirectly pleiotropically influence epigenetic states of multiple regions on other chromosomes with which it interacts...
  15. pmc The binding sites for the chromatin insulator protein CTCF map to DNA methylation-free domains genome-wide
    Rituparna Mukhopadhyay
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Genome Res 14:1594-602. 2004
    ..As these targets are generally DNA methylation-free as determined by antibodies against 5-methylcytidine and a methyl-binding protein (MBD2), a CTCF-based network correlates with genome-wide epigenetic states...
  16. pmc Multiple nucleosome positioning sites regulate the CTCF-mediated insulator function of the H19 imprinting control region
    Meena Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Mol Cell Biol 22:3339-44. 2002
    ..We propose that the NPSs ensure the fidelity of the repressed status of the maternal Igf2 allele during development by constitutively maintaining availability of the CTCF target sites...
  17. ncbi request reprint The kinetics of deregulation of expression by de novo methylation of the h19 imprinting control region in cancer cells
    Chandrasekhar Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Cancer Res 62:4545-8. 2002
    ..Our results document that a strong de novo methylation machinery leads to loss of methylation privilege states of H19 ICR to functionally manifest loss of insulator function in a matter of only a few days in human cancer cells...
  18. ncbi request reprint The 4C technique: the 'Rosetta stone' for genome biology in 3D?
    Rolf Ohlsson
    Department of Development and Genetics, Norbyvagen 18A, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Curr Opin Cell Biol 19:321-5. 2007
    ..As this method is based on the unbiased identification of sequences interacting with specific baits, there are unique opportunities for unravelling the secrets of how the genome functions in 3D...
  19. ncbi request reprint Epigenetic variability and the evolution of human cancer
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Adv Cancer Res 88:145-68. 2003
    ....
  20. pmc Chromatin insulators and cohesins
    Anita Göndör
    Department of Development and Genetics, Uppsala University, Evolution Biology Centre, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    EMBO Rep 9:327-9. 2008
  21. ncbi request reprint A differentially methylated imprinting control region within the Kcnq1 locus harbors a methylation-sensitive chromatin insulator
    Chandrasekhar Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    J Biol Chem 277:18106-10. 2002
    ..Moreover, we document that CpG methylation regulates this insulator function suggesting that a methylation-sensitive chromatin insulator is a common theme in the phenomenon of genomic imprinting...
  22. doi request reprint High-resolution circular chromosome conformation capture assay
    Anita Göndör
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Nat Protoc 3:303-13. 2008
    ..Following optimization of enzyme digestions and amplification conditions, the protocol can be completed in 2-3 weeks...
  23. ncbi request reprint Multiple cis elements within the Igf2/H19 insulator domain organize a distance-dependent silencer. A cautionary note
    Vasudeva Ginjala
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    J Biol Chem 277:5707-10. 2002
    ..We issue a cautionary note, therefore, that the interpretation of insulator/silencer data could be erroneous should the distance issue not be taken into consideration...
  24. ncbi request reprint Loss of IGF2 imprinting: mechanisms and consequences
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Novartis Found Symp 262:108-21; discussion 121-4, 265-8. 2004
    ..We discuss here the possibility that pathological mechanisms leading to IGF2 LOI use different strategies with the neutralization of the H19 ICR insulator function as a common denominator...
  25. ncbi request reprint New twists on the epigenetics of CpG islands
    Rolf Ohlsson
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Genome Res 12:525-6. 2002
  26. doi request reprint Replication timing and epigenetic reprogramming of gene expression: a two-way relationship?
    Anita Göndör
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
    Nat Rev Genet 10:269-76. 2009
    ..These considerations have implications for our understanding of development, evolution and diseases such as cancer...
  27. ncbi request reprint Genetics. Widespread monoallelic expression
    Rolf Ohlsson
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Science 318:1077-8. 2007
  28. doi request reprint Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution
    Xingqi Chen
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Biotechniques 56:117-8, 120-4. 2014
    ..We use this method to identify higher order structures of individual chromosomes in relation to structural hallmarks of interphase nuclei and beyond the resolution of the light microscope. ..
  29. pmc FOXP3 promoter demethylation reveals the committed Treg population in humans
    Peter C J Janson
    Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 3:e1612. 2008
    ....
  30. doi request reprint Chromosome crosstalk in three dimensions
    Anita Göndör
    Department of Microbiology, Tumor and Cell Biology, Nobels vag 16, Box 280, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Nature 461:212-7. 2009
    ..Technical advances in detecting these interactions contribute to our understanding of the functional organization of the genome, as well as its adaptive plasticity in response to environmental changes during development and disease...