Pär Nordlund

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi Ribonucleotide reductases
    Pär Nordlund
    Division of Biophysics and 2Division of Biochemistry, Medical Nobel Institute, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Annu Rev Biochem 75:681-706. 2006
  2. doi Substrate specificity and oligomerization of human GMP synthetase
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    J Mol Biol 425:4323-33. 2013
  3. doi Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS J 277:4920-30. 2010
  4. pmc Structures of BIR domains from human NAIP and cIAP2
    Maria Dolores Herman
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:1091-6. 2009
  5. ncbi Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase
    Daniel Martinez Molina
    Division of Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Nature 448:613-6. 2007
  6. pmc Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy
    Petri Kursula
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:613-7. 2006
  7. pmc Structural studies of tri-functional human GART
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Nucleic Acids Res 38:7308-19. 2010
  8. pmc Expression, purification, crystallization and preliminary X-ray analysis of ORF60, the small subunit (R2) of ribonucleotide reductase from Kaposi's sarcoma-associated herpesvirus (KSHV)
    Daniel Gurmu
    Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles vag 2, SE 171 77 Stockholm, Sweden
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:734-7. 2010
  9. pmc Arginine 104 is a key catalytic residue in leukotriene C4 synthase
    Agnes Rinaldo-Matthis
    Division of Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 285:40771-6. 2010
  10. pmc Structural insights into substrate recognition in proton-dependent oligopeptide transporters
    Fatma Guettou
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177 Stockholm, Sweden
    EMBO Rep 14:804-10. 2013

Collaborators

Detail Information

Publications39

  1. ncbi Ribonucleotide reductases
    Pär Nordlund
    Division of Biophysics and 2Division of Biochemistry, Medical Nobel Institute, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Annu Rev Biochem 75:681-706. 2006
    ..In spite of large differences in the amino acid sequences, basic structural features are remarkably similar and suggest a common evolutionary origin for the three classes...
  2. doi Substrate specificity and oligomerization of human GMP synthetase
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    J Mol Biol 425:4323-33. 2013
    ..The information obtained from the crystal structure of human GMPS might therefore aid in understanding interactions of nucleoside-based drugs with GMPS and in structure-based design of GMPS-specific inhibitors. ..
  3. doi Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS J 277:4920-30. 2010
    ..The data obtained in the present study indicate that PRTFDC1 does not have a direct catalytic role in the nucleotide salvage pathway...
  4. pmc Structures of BIR domains from human NAIP and cIAP2
    Maria Dolores Herman
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:1091-6. 2009
    ..In addition to shedding light on the specificity determinants of these two proteins, the structures should now also provide a framework for future structure-based work targeting these proteins...
  5. ncbi Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase
    Daniel Martinez Molina
    Division of Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Nature 448:613-6. 2007
    ....
  6. pmc Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy
    Petri Kursula
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:613-7. 2006
    ..The structure also serves as a basis for structure-based design of anti-proliferative inhibitors...
  7. pmc Structural studies of tri-functional human GART
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Nucleic Acids Res 38:7308-19. 2010
    ..This resilient seesaw shape may facilitate internal substrate/product transfer or forwarding to other enzymes in the pathway...
  8. pmc Expression, purification, crystallization and preliminary X-ray analysis of ORF60, the small subunit (R2) of ribonucleotide reductase from Kaposi's sarcoma-associated herpesvirus (KSHV)
    Daniel Gurmu
    Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles vag 2, SE 171 77 Stockholm, Sweden
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:734-7. 2010
    ..9, b = 71.2, c = 71.8 A, alpha = 90, beta = 106.7, gamma = 90 degrees. The data set collected was 95.3% complete, with an R(merge) of 9.6%. There are two molecules in the asymmetric unit, corresponding to a solvent content of 43.4%...
  9. pmc Arginine 104 is a key catalytic residue in leukotriene C4 synthase
    Agnes Rinaldo-Matthis
    Division of Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 285:40771-6. 2010
    ..Because Arg-104 is a conserved residue, our results pertain to other homologous membrane proteins and represent a structure-function paradigm probably common to all microsomal GSH transferases...
  10. pmc Structural insights into substrate recognition in proton-dependent oligopeptide transporters
    Fatma Guettou
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177 Stockholm, Sweden
    EMBO Rep 14:804-10. 2013
    ..All ligand-binding residues are highly conserved and the structural insights presented here are therefore likely to also apply to human POTs...
  11. pmc The crystal structure of the Dachshund domain of human SnoN reveals flexibility in the putative protein interaction surface
    Tomas Nyman
    Structural Genomics Consortium, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 5:e12907. 2010
    ..The flexibility observed in the putative protein binding groove may enable SnoN to recognize multiple interaction partners...
  12. ncbi Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms
    Esben M Quistgaard
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177 Stockholm, Sweden Electronic address
    J Struct Biol 184:375-8. 2013
    ....
  13. doi Completing the family portrait of the anti-apoptotic Bcl-2 proteins: crystal structure of human Bfl-1 in complex with Bim
    Maria Dolores Herman
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Pär Nordlund, 17177 Stockholm, Sweden
    FEBS Lett 582:3590-4. 2008
    ....
  14. ncbi The crystal structure of the bifunctional deaminase/reductase RibD of the riboflavin biosynthetic pathway in Escherichia coli: implications for the reductive mechanism
    Pal Stenmark
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Mol Biol 373:48-64. 2007
    ....
  15. ncbi Crystal structure of human inosine triphosphatase. Substrate binding and implication of the inosine triphosphatase deficiency mutation P32T
    Pal Stenmark
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, 17177 Stockholm, Sweden
    J Biol Chem 282:3182-7. 2007
    ..We predict that the ITPA deficiency mutation P32T leads to a shift of this loop that results in a disturbed affinity for nucleotides and/or a reduced catalytic activity in both monomers of the physiological dimer...
  16. ncbi Structural basis for phosphoinositide substrate recognition, catalysis, and membrane interactions in human inositol polyphosphate 5-phosphatases
    Lionel Trésaugues
    Structural Genomics Consortium, Karolinska Institutet, 17177 Stockholm, Sweden
    Structure 22:744-55. 2014
    ..The high conservation of the environment of the scissile 5-phosphate suggests a common reaction geometry for all members of the human 5-phosphatase family. ..
  17. pmc Structural and biophysical characterization of the cytoplasmic domains of human BAP29 and BAP31
    Esben M Quistgaard
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 8:e71111. 2013
    ..2 in vitro. It is however likely that folding of the domain is triggered by other factors than low pH in vivo. We found no evidence for direct interaction of the cytoplasmic domains of BAP29 and BAP31. ..
  18. doi The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins
    Susanne Gräslund
    Structural Genomics Consortium, Karolinska Institutet, Department of Medical Biophysics and Biochemistry, 17177 Stockholm, Sweden
    Protein Expr Purif 58:210-21. 2008
    ..Finally, a retrospective analysis of gel filtration profiles indicates that these could be used with caution to prioritize protein targets for crystallization trials...
  19. doi Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters
    Esben M Quistgaard
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Nat Struct Mol Biol 20:766-8. 2013
    ..This is, to our knowledge, the first MFS transporter structure determined in more than one conformational state, which may establish XylE as an important MFS model protein...
  20. doi High-throughput analytical gel filtration screening of integral membrane proteins for structural studies
    Christian Löw
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Biochim Biophys Acta 1830:3497-508. 2013
    ..However, these approaches suffer from two drawbacks; proteins with a translocated C-terminus cannot be tested and scale-up from analytical to preparative purification is often non-trivial and may require re-cloning...
  21. doi High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris
    Anders Wetterholm
    Department of Medical Biochemistry and Biophysics, Division for Chemistry 2, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Protein Expr Purif 60:1-6. 2008
    ..Hence, our approach holds promise for solving the structure of rat LTC4S and other members of the MAPEG family of integral membrane proteins...
  22. ncbi Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolution
    Said Eshaghi
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Science 313:354-7. 2006
    ....
  23. ncbi Understanding specificity in metabolic pathways--structural biology of human nucleotide metabolism
    Martin Welin
    Structural Genomics Consortium, Karolinska Institutet, 17177 Stockholm, Sweden
    Biochem Biophys Res Commun 396:157-63. 2010
    ....
  24. ncbi Crystal structure of YegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site
    H M Amin Bakali
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    J Biol Chem 282:19644-52. 2007
    ..The active site is located in the interdomain cleft formed by four conserved sequence motifs. Surprisingly, the structure reveals a novel metal binding site composed of residues conserved in most lipid kinases...
  25. doi Crystal structure of the shutoff and exonuclease protein from the oncogenic Kaposi's sarcoma-associated herpesvirus
    Sue Li Dahlroth
    Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS J 276:6636-45. 2009
    ..Mutations specifically affecting host shutoff map to a surface region of the N-terminal domain, implying an essential role in protein-protein interactions, and link the RNase activity of the enzyme to mRNA degradation pathways...
  26. ncbi Catalysis within the lipid bilayer-structure and mechanism of the MAPEG family of integral membrane proteins
    Daniel Martinez Molina
    Department of Medical Biochemistry and Biophysics, Division of Biophysics, Karolinska Institutet, Stockholm, Sweden
    Curr Opin Struct Biol 18:442-9. 2008
    ..Structure-based sequence alignments of human MAPEG members support the notion that the glutathione binding site is highly conserved among MAPEG enzymes and that they use a similar mechanism for glutathione activation...
  27. doi High-resolution insights into binding of unfolded polypeptides by the PPIase chaperone SlpA
    Esben M Quistgaard
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles vag 2, SE 171 77 Stockholm, Sweden
    FASEB J 26:4003-13. 2012
    ..Based on pulldown results, we suggest that SlpA is likely to be involved in ribosome assembly...
  28. pmc Nanobody mediated crystallization of an archeal mechanosensitive channel
    Christian Löw
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 8:e77984. 2013
    ..Together with previous data we show that nanobodies are a versatile and valuable tool in structural biology by widening the crystallization space for highly challenging proteins, protein complexes and integral membrane proteins. ..
  29. pmc The crystal structure of the DNA-binding domain of vIRF-1 from the oncogenic KSHV reveals a conserved fold for DNA binding and reinforces its role as a transcription factor
    Kelly Hew
    Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 138673 Singapore, Singapore
    Nucleic Acids Res 41:4295-306. 2013
    ....
  30. ncbi The structure of human collapsin response mediator protein 2, a regulator of axonal growth
    Pal Stenmark
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    J Neurochem 101:906-17. 2007
    ..The differences in oligomerisation interfaces between CRMP-1 and CRMP-2 are used to model CRMP-1/2 heterotetramers...
  31. doi Structural basis for the allosteric regulation and substrate recognition of human cytosolic 5'-nucleotidase II
    Karin Wallden
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    J Mol Biol 408:684-96. 2011
    ..These structures provide a comprehensive structural basis for the design of cN-II inhibitors. They also contribute to the understanding of how the nucleotide salvage pathway is regulated at a molecular level...
  32. ncbi Structures of the hydrolase domain of human 10-formyltetrahydrofolate dehydrogenase and its complex with a substrate analogue
    Petri Kursula
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    Acta Crystallogr D Biol Crystallogr 62:1294-9. 2006
    ..Two water molecules in the structures mark the positions of key oxygen moieties in the catalytic reaction and reaction geometries are proposed based on the structural data...
  33. ncbi Functional properties and molecular architecture of leukotriene A4 hydrolase, a pivotal catalyst of chemotactic leukotriene formation
    Jesper Z Haeggstrom
    Department of Medical Biochemistry and Biophysics, Division of Chemistry 2, Karolinska Institutet, S 171 77 Stockholm, Sweden
    ScientificWorldJournal 2:1734-49. 2002
    ..Here we describe the most recent developments regarding our understanding of the function and molecular architecture of leukotriene A4 hydrolase...
  34. pmc Optimisation of over-expression in E. coli and biophysical characterisation of human membrane protein synaptogyrin 1
    Christian Löw
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 7:e38244. 2012
    ..The protein stability is significantly improved in the presence of cholesteryl hemisuccinate as judged by differential light scattering. The approach described here can easily be adapted to other eukaryotic IMPs...
  35. pmc Protein production and purification
    Susanne Gräslund
    Karolinska Institutet, Scheeles vag 2, 171 77 Stockholm, Sweden
    Nat Methods 5:135-46. 2008
    ..This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators...
  36. ncbi Structural and Biochemical Characterization of Human PR70 in Isolation and in Complex with the Scaffolding Subunit of Protein Phosphatase 2A
    Rebecca Dovega
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 9:e101846. 2014
    ..This study provides additional knowledge about the structural basis for the function of B'' containing holoenzymes. ..
  37. ncbi GRETA, a new multifermenter system for structural genomics and process optimization
    Marie Hedrén
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Acta Crystallogr D Biol Crystallogr 62:1227-31. 2006
    ....
  38. doi Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay
    Daniel Martinez Molina
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Science 341:84-7. 2013
    ..CETSA is likely to become a valuable tool for the validation and optimization of drug target engagement. ..
  39. ncbi The structure of the RNA m5C methyltransferase YebU from Escherichia coli reveals a C-terminal RNA-recruiting PUA domain
    B Martin Hallberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, 17177 Stockholm, Sweden
    J Mol Biol 360:774-87. 2006
    ..The ribosomal protein interactions would explain why the assembled 30 S subunit, and not naked 16 S rRNA, is the preferred substrate for YebU...