H Jornvall

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi request reprint Multiplicity of eukaryotic ADH and other MDR forms
    Hans Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77, Stockholm, Sweden
    Chem Biol Interact 143:255-61. 2003
  2. ncbi request reprint Variations and constant patterns in eukaryotic MDR enzymes. Conclusions from novel structures and characterized genomes
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77, Stockholm, Sweden
    Chem Biol Interact 130:491-8. 2001
  3. ncbi request reprint SDR and MDR: completed genome sequences show these protein families to be large, of old origin, and of complex nature
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 445:261-4. 1999
  4. ncbi request reprint Isolation of three antibacterial peptides from pig intestine: gastric inhibitory polypeptide (7-42), diazepam-binding inhibitor (32-86) and a novel factor, peptide 3910
    B Agerberth
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 216:623-9. 1993
  5. ncbi request reprint Characterization of dopuin, a polypeptide with special residue distributions
    Z W Chen
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 249:518-22. 1997
  6. ncbi request reprint Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids
    H U Marschall
    Karolinska Institutet, Division of Gastroenterology and Hepatology, Huddinge University Hospital, SE 141 86 Huddinge, Sweden
    Hepatology 31:990-6. 2000
  7. pmc NK-lysin, a novel effector peptide of cytotoxic T and NK cells. Structure and cDNA cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity
    M Andersson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    EMBO J 14:1615-25. 1995
  8. pmc Escherichia coli ferredoxin NADP+ reductase: activation of E. coli anaerobic ribonucleotide reduction, cloning of the gene (fpr), and overexpression of the protein
    V Bianchi
    Department of Biochemistry I, Karolinska Institutet, Stockholm, Sweden
    J Bacteriol 175:1590-5. 1993
  9. ncbi request reprint Acetylated N-terminal structures of class III alcohol dehydrogenases. Differences among the three enzyme classes
    T Fairwell
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 222:99-103. 1987
  10. ncbi request reprint Identification of the bioactive peptide PEC-60 in brain
    A Norberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden
    Cell Mol Life Sci 60:378-81. 2003

Collaborators

Detail Information

Publications126 found, 100 shown here

  1. ncbi request reprint Multiplicity of eukaryotic ADH and other MDR forms
    Hans Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77, Stockholm, Sweden
    Chem Biol Interact 143:255-61. 2003
    ....
  2. ncbi request reprint Variations and constant patterns in eukaryotic MDR enzymes. Conclusions from novel structures and characterized genomes
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77, Stockholm, Sweden
    Chem Biol Interact 130:491-8. 2001
    ....
  3. ncbi request reprint SDR and MDR: completed genome sequences show these protein families to be large, of old origin, and of complex nature
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 445:261-4. 1999
    ..Repeated duplications can be traced for the alcohol dehydrogenases, with independent enzymogenesis of ethanol activity, showing a general importance of this enzyme activity...
  4. ncbi request reprint Isolation of three antibacterial peptides from pig intestine: gastric inhibitory polypeptide (7-42), diazepam-binding inhibitor (32-86) and a novel factor, peptide 3910
    B Agerberth
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 216:623-9. 1993
    ..All three peptides showed good activity against B. megaterium. In addition, GIP (7-42) showed some activity against Streptococcus pyogenes and an Escherichia coli mutant with a defect in its outer membrane...
  5. ncbi request reprint Characterization of dopuin, a polypeptide with special residue distributions
    Z W Chen
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 249:518-22. 1997
    ..These two peptides increase the complexity of known intestinal polypeptides and at least dopuin has properties compatible with specific biofunctions...
  6. ncbi request reprint Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids
    H U Marschall
    Karolinska Institutet, Division of Gastroenterology and Hepatology, Huddinge University Hospital, SE 141 86 Huddinge, Sweden
    Hepatology 31:990-6. 2000
    ..Hydroxysteroid dehydrogenases are candidates for the binding and transport of 3alpha-hydroxy bile acids. We assume that ADH gammagamma isozyme is involved in cytosolic bile acid binding and transport processes as well...
  7. pmc NK-lysin, a novel effector peptide of cytotoxic T and NK cells. Structure and cDNA cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity
    M Andersson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    EMBO J 14:1615-25. 1995
    ..We suggest that NK-lysin is a new effector molecule of cytotoxic T and NK cells...
  8. pmc Escherichia coli ferredoxin NADP+ reductase: activation of E. coli anaerobic ribonucleotide reduction, cloning of the gene (fpr), and overexpression of the protein
    V Bianchi
    Department of Biochemistry I, Karolinska Institutet, Stockholm, Sweden
    J Bacteriol 175:1590-5. 1993
    ..Morimyo, J. Bacteriol. 170:2136-2142, 1988). We suggest that a frameshift introduced during isolation or sequencing of mvrA caused an error in the determination of its sequence...
  9. ncbi request reprint Acetylated N-terminal structures of class III alcohol dehydrogenases. Differences among the three enzyme classes
    T Fairwell
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 222:99-103. 1987
    ....
  10. ncbi request reprint Identification of the bioactive peptide PEC-60 in brain
    A Norberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden
    Cell Mol Life Sci 60:378-81. 2003
    ..The specific regional brain distribution and interaction with classical neurotransmitters raise the possibility that PEC-60 may play a role in the central nervous system disorders involving dopamine dysregulation...
  11. pmc Molecular cloning and amino acid sequence of human 5-lipoxygenase
    T Matsumoto
    Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 85:26-30. 1988
    ..RNA blot analysis indicated substantial amounts of an mRNA species of approximately equal to 2700 nucleotides in leukocytes, lung, and placenta...
  12. ncbi request reprint An ethanol-inducible MDR ethanol dehydrogenase/acetaldehyde reductase in Escherichia coli: structural and enzymatic relationships to the eukaryotic protein forms
    J Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 263:305-11. 1999
    ..Although present in several strains under different conditions, inducibility may constitute an explanation for the fairly late characterization of this E. coli gene product...
  13. pmc Two alternative processing pathways for a preprohormone: a bioactive form of secretin
    V Bonetto
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 92:11985-9. 1995
    ....
  14. ncbi request reprint Gln-Gly cleavage: correlation between collision-induced dissociation and biological degradation
    A P Jonsson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    J Am Soc Mass Spectrom 12:337-42. 2001
    ..We suggest a link between the gas-phase chemistry and the biochemical degradation of these molecules...
  15. ncbi request reprint Avian alcohol dehydrogenase: the chicken liver enzyme. Primary structure, cDNA-cloning, and relationships to other alcohol dehydrogenases
    M Estonius
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 194:593-602. 1990
    ..One of the residues, Gly260 (mammalian class I numbering system), previously considered characteristic of all alcohol dehydrogenases, is replaced by Gln...
  16. pmc A possible glycine radical in anaerobic ribonucleotide reductase from Escherichia coli: nucleotide sequence of the cloned nrdD gene
    X Sun
    Department of Biochemistry I, Karolinska Institute, Stockholm, Sweden
    Proc Natl Acad Sci U S A 90:577-81. 1993
    ..F. V., Frey, M., Neugebauer, F. A., Schäfer, W. & Knappe, J. (1992) Proc. Natl. Acad. Sci. USA 89, 996-1000]. The gene for the anaerobic reductase is located at a position around 96 min on the E. coli genomic map...
  17. pmc Mammalian class IV alcohol dehydrogenase (stomach alcohol dehydrogenase): structure, origin, and correlation with enzymology
    X Pares
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 91:1893-7. 1994
    ..Clearly, class IV is distinct in structure, ethanol turnover, stomach expression, and possible emergence from class I...
  18. ncbi request reprint Hydrophobic 3.7 kDa surfactant polypeptide: structural characterization of the human and bovine forms
    J Johansson
    Department of Clinical Chemistry, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden
    FEBS Lett 232:61-4. 1988
    ..Comparison of the porcine, human and bovine polypeptides reveals a conserved hydrophobic middle/C-terminal segment and a variable hydrophilic N-terminal part...
  19. ncbi request reprint Decreased pulmonary levels of the anti-inflammatory Clara cell 16 kDa protein after induction of airway inflammation in asthmatics
    C Lensmar
    Department of Medicine, Karolinska Hospital, Stockholm, Sweden
    Cell Mol Life Sci 57:976-81. 2000
    ..Reduced levels of pulmonary CC16 may cause loss of anti-inflammatory activity in the airways and contribute to the development of airway inflammation in asthma...
  20. pmc Fundamental molecular differences between alcohol dehydrogenase classes
    O Danielsson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 91:4980-4. 1994
    ....
  21. ncbi request reprint Isolation and characterization of porcine diazepam-binding inhibitor, a polypeptide not only of cerebral occurrence but also common in intestinal tissues and with effects on regulation of insulin release
    Z W Chen
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 174:239-45. 1988
    ..It is possible that DBI belongs to a new family of gut polypeptides which inhibit glucose-mediated insulin release by hormonal and/or neurocrine mechanisms...
  22. ncbi request reprint Identification of reactive tyrosine residues in cysteine-reactive dehydrogenases. Differences between liver sorbitol, liver alcohol and Drosophila alcohol dehydrogenases
    V Prozorovski
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 304:46-50. 1992
    ..Super-activation with cyanide was also noticed after S-sulphocysteine protection. The results demonstrate the possibility of identification of specific Tyr residues in proteins with reversibly protected Cys residues...
  23. ncbi request reprint Hep27, a member of the short-chain dehydrogenase/reductase family, is an NADPH-dependent dicarbonyl reductase expressed in vascular endothelial tissue
    N Shafqat
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77, Stockholm, Sweden
    Cell Mol Life Sci 63:1205-13. 2006
    ....
  24. ncbi request reprint Ser(13)-phosphorylated PYY from porcine intestine with a potent biological activity
    Z Chen
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 492:119-22. 2001
    ..5 nM compared to 0.15 nM for non-phosphorylated PYY. The finding of phosphorylation of PYY is unusual among hormonal peptides, and emphasizes the importance of direct protein analysis of gene products...
  25. pmc Identification, isolation, and characterization of daintain (allograft inflammatory factor 1), a macrophage polypeptide with effects on insulin secretion and abundantly present in the pancreas of prediabetic BB rats
    Z W Chen
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 94:13879-84. 1997
    ....
  26. ncbi request reprint The human antimicrobial and chemotactic peptides LL-37 and alpha-defensins are expressed by specific lymphocyte and monocyte populations
    B Agerberth
    Department of Medical Biochemistry and Biophysics, Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden
    Blood 96:3086-93. 2000
    ....
  27. ncbi request reprint Sorbitol dehydrogenase: cDNA coding for the rat enzyme. Variations within the alcohol dehydrogenase family independent of quaternary structure and metal content
    C Karlsson
    Department of Chemistry I, Katolinska Institutet, Stockholm, Sweden
    Eur J Biochem 198:761-5. 1991
    ....
  28. ncbi request reprint Function, gene organization and protein structures of 11beta-hydroxysteroid dehydrogenase isoforms
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 249:355-60. 1997
    ..On the other hand, for adrenal glucocorticoids the type-2 enzyme seems to be exclusively a dehydrogenase and, by inactivating glucocorticoids, confers specificity to peripheral mineralocorticoid receptors...
  29. ncbi request reprint Unordered structured of proinsulin C-peptide in aqueous solution and in the presence of lipid vesicles
    M Henriksson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Cell Mol Life Sci 57:337-42. 2000
    ..In conclusion, C-peptide is unlikely to elicit physiological effects through stable conformation-dependent interactions with lipid membranes...
  30. ncbi request reprint Primary structure of the hemoglobin alpha-chain of rose-ringed parakeet (Psittacula krameri)
    A Islam
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    J Protein Chem 7:561-9. 1988
    ....
  31. ncbi request reprint Isolation and characterization of hydrophobic polypeptides in human bile
    M Stark
    Department of Medical Biochemistry, Karolinska Institutet, Stockholm, Sweden Margareta
    Eur J Biochem 266:209-14. 1999
    ..Several of the polypeptides found are unexpectedly present in bile, suggesting that specific excretion mechanisms may be involved...
  32. ncbi request reprint Distribution of alcohol and sorbitol dehydrogenases. Assessment of mRNA species in mammalian tissues
    M Estonius
    Department of Chemistry I, Karolinska Instituet, Stockholm, Sweden
    Eur J Biochem 215:497-503. 1993
    ..In addition, evidence for a sex-specific expression, in the liver, of class-II alcohol dehydrogenase was obtained...
  33. pmc Carboxyethyllysine in a protein: native carbonyl reductase/NADP(+)-dependent prostaglandin dehydrogenase
    M Krook
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 90:502-6. 1993
    ....
  34. ncbi request reprint Three-dimensional model of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase and relationships to the NADP(+)-dependent enzyme (carbonyl reductase)
    M Krook
    Department of Chemistry I, Karolinska Institute, Stockholm, Sweden
    FEBS Lett 322:139-42. 1993
    ..Active site relationships can be evaluated and subunit interactions predicted, suggesting that the alpha E + alpha F two-helix surface constitutes the major subunit interacting area, forming a dimeric unit in the oligomeric enzymes...
  35. ncbi request reprint Isozyme developments in mammalian class-I alcohol dehydrogenase. cDNA cloning, functional correlations, and lack of evidence for genetic isozymes in rabbit
    J O Hoog
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 213:31-8. 1993
    ..1 mM, for ethanol 1.6-1.9 mM). The isozyme versus species differences mark the variability of class-I alcohol dehydrogenase versus class III and suggest a parallelism between rapid mutational differences and frequent duplicatory events...
  36. ncbi request reprint Rat liver alcohol dehydrogenase of class III. Primary structure, functional consequences and relationships to other alcohol dehydrogenases
    P Julia
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 172:73-83. 1988
    ..Finally the class III structure supports evolutionary relationships suggesting that the three classes constitute clearly separate enzymes within the group of mammalian zinc-containing alcohol dehydrogenases...
  37. ncbi request reprint ClpB proteins copurify with the anaerobic Escherichia coli reductase
    E Pontis
    Department of Biochemistry I, Karolinska Institute, Stockholm, Sweden
    Biochem Biophys Res Commun 180:1222-6. 1991
    ..The tight associations suggest the possibility that the ClpB proteins are involved in the regulation of the anaerobic reductase...
  38. ncbi request reprint Class III human liver alcohol dehydrogenase: a novel structural type equidistantly related to the class I and class II enzymes
    R Kaiser
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Biochemistry 27:1132-40. 1988
    ..abstract truncated at 250 words)..
  39. ncbi request reprint Short-chain dehydrogenases/reductases (SDR)
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Biochemistry 34:6003-13. 1995
    ..Short-chain dehydrogenases illustrate the value of comparisons and diversified research in generating unexpected discoveries...
  40. ncbi request reprint Isolation and structural characterization of porcine coupling factor 6 from intestinal tissues
    Z W Chen
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 226:43-6. 1987
    ..The extensive conservation suggests strict structural constraints on the functional properties of the polypeptide...
  41. ncbi request reprint Recovery of gel-separated proteins for in-solution digestion and mass spectrometry
    A P Jonsson
    Department of Medical Biochemistry and Biophysics, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden
    Anal Chem 73:5370-7. 2001
    ..The improved ability to localize and determine protein modifications is shown for N-terminal acetylation and methylation of a histidine residue. Furthermore, the method enables fast screening of homologous protein sequences...
  42. ncbi request reprint Amino acid sequence of PR-39. Isolation from pig intestine of a new member of the family of proline-arginine-rich antibacterial peptides
    B Agerberth
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 202:849-54. 1991
    ..The peptide was named PR-39 (proline-arginine-rich with a size of 39 residues). The lethal concentration of the peptide was determined against six Gram-negative and four Gram-positive strains of bacteria...
  43. pmc Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme
    G Spyrou
    Department of Biochemistry, Karolinska Institutet, Stockholm, Sweden
    J Bacteriol 173:3673-9. 1991
    ....
  44. ncbi request reprint Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus. Co-purification of RNase A with Sso7c
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    FEBS Lett 432:141-4. 1998
    ....
  45. ncbi request reprint Degradation of proinsulin C-peptide in kidney and placenta extracts by a specific endoprotease activity
    E Melles
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden
    Cell Mol Life Sci 61:2979-82. 2004
    ..We conclude that the degradation of C-peptide in kidney and placenta follows similar patterns, dominated by endopeptidase cleavages N-terminal of Leu...
  46. ncbi request reprint Characteristics of short-chain alcohol dehydrogenases and related enzymes
    B Persson
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 200:537-43. 1991
    ..Consequently, the overall comparisons suggest the possibility of related mechanisms and domain properties for different members of the short-chain family...
  47. ncbi request reprint Gln-Gly cleavage: a dominant dissociation site in the fragmentation of protonated peptides
    A P Jonsson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Rapid Commun Mass Spectrom 15:713-20. 2001
    ....
  48. ncbi request reprint Sheep neuropeptide Y. A third structural type of a highly conserved peptide
    R Sillard
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 258:263-5. 1989
    ..At position 17 it has Leu as do both pig and ox NPY. Consequently, 3 different structural types of mammalian NPY are now known...
  49. ncbi request reprint Variability within mammalian sorbitol dehydrogenases. The primary structure of the human liver enzyme
    C Karlsson
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 186:543-50. 1989
    ..The primary structure of human liver sorbitol dehydrogenase further shows that the absence of three of the four ligands to a second zinc atom present in alcohol dehydrogenases is a general property of sorbitol dehydrogenase...
  50. ncbi request reprint Primary structure of glycolate oxidase from spinach
    E Cederlund
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 173:523-30. 1988
    ..An amino acid sequence homology with flavocytochrome b2 from yeast, as expected from known similarities in tertiary structure, is observed (33% residue identities)...
  51. pmc Molecular cloning and amino acid sequence of leukotriene A4 hydrolase
    C D Funk
    Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 84:6677-81. 1987
    ..No apparent homologies with microsomal epoxide hydrolases were found. RNA blot analysis indicated substantial amounts of a discrete mRNA of approximately equal to 2250 nucleotides in lung tissue and leukocytes...
  52. ncbi request reprint Ethanol-, fasting-, and acetone-inducible cytochromes P-450 in rat liver: regulation and characteristics of enzymes belonging to the IIB and IIE gene subfamilies
    I Johansson
    Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden
    Biochemistry 27:1925-34. 1988
    ..The results indicate that effects of ethanol, acetone, and/or starvation on drug and xenobiotic metabolism are caused by the induction of P-450 forms belonging to at least two gene subfamilies...
  53. pmc Hydrophobic surfactant-associated polypeptides: SP-C is a lipopeptide with two palmitoylated cysteine residues, whereas SP-B lacks covalently linked fatty acyl groups
    T Curstedt
    Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden
    Proc Natl Acad Sci U S A 87:2985-9. 1990
    ..Long-chain acylation may constitute a means for association of proteins with membranes and could conceivably modulate the stability and biological activity of surfactant films...
  54. ncbi request reprint T cell tolerance based on avidity thresholds rather than complete deletion allows maintenance of maximal repertoire diversity
    J K Sandberg
    Microbiology and Tumor Biology Center and Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    J Immunol 165:25-33. 2000
    ..We interpret these data, together with results previously published by others, in an avidity pit model based on avidity thresholds for maintenance of both maximal diversity and optimal self tolerance in the CD8+ T cell repertoire...
  55. ncbi request reprint Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-metalloenzyme' alcohol dehydrogenase type
    L Marekov
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 266:51-4. 1990
    ..These enzymes are low in Cys and have a strictly conserved Tyr residue that appears to be important...
  56. ncbi request reprint Glutaredoxin-3 from Escherichia coli. Amino acid sequence, 1H AND 15N NMR assignments, and structural analysis
    F Aslund
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 171 77 Stockholm, Sweden
    J Biol Chem 271:6736-45. 1996
    ..Thus, despite an identical active site disulfide motif and a similar secondary structure and tertiary fold, Grx3 and Grx1 display large functional differences in in vitro protein disulfide oxido-reduction reactions...
  57. ncbi request reprint Short-chain dehydrogenases. Proteolysis and chemical modification of prokaryotic 3 alpha/20 beta-hydroxysteroid, insect alcohol and human 15-hydroxyprostaglandin dehydrogenases
    M Krook
    Department of Chemistry I, Karolinska Institutet, Sweden
    Eur J Biochem 209:233-9. 1992
    ....
  58. ncbi request reprint Porphobilinogen deaminase in human erythrocytes: purification of two forms with apparent molecular weights of 40 kDa and 42 kDa
    L Lannfelt
    Department of Psychiatry, St Goran s Hospital, Stockholm, Sweden
    Scand J Clin Lab Invest 49:677-84. 1989
    ..The two forms present could each be separated into three differently charged subforms by Mono Q chromatography...
  59. ncbi request reprint Cellular internalization of proinsulin C-peptide
    E Lindahl
    Department of Medical Biochemistry and Biophysics MBB, Karolinska Institutet, 171 77, Stockholm, Sweden
    Cell Mol Life Sci 64:479-86. 2007
    ..Hence, the data suggest the possibility of one further C-peptide site of action...
  60. pmc Processing of prosecretin: isolation of a secretin precursor from porcine intestine
    G Gafvelin
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 87:6781-5. 1990
    ..This implies that differential splicing may occur when the secretin gene transcript is processed to mRNA...
  61. pmc Isolation and characterization of a 60-residue intestinal peptide structurally related to the pancreatic secretory type of trypsin inhibitor: influence on insulin secretion
    B Agerberth
    Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden
    Proc Natl Acad Sci U S A 86:8590-4. 1989
    ..The biological role of PEC-60 is not known, but the effect on insulin secretion and the homologies observed suggest important biological activities and interesting structural relationships...
  62. ncbi request reprint Pharmacogenetics of the alcohol dehydrogenase system
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Pharmacology 61:184-91. 2000
    ..Future perspectives suggest continued studies in specific directions for distinction of metabolic, regulatory and pharmacogenetic roles of ADH...
  63. ncbi request reprint A novel Ser O-glucuronidation in acidic proline-rich proteins identified by tandem mass spectrometry
    A P Jonsson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 475:131-4. 2000
    ..The latter modification was located to Ser-17 and found to be present in approximately 40% of the polypeptides...
  64. ncbi request reprint Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes
    T Izumi
    Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 202:1231-8. 1991
    ..Of particular interest, the relative amounts of the two forms differed markedly between leukocytes obtained from normal donors and leukocytes from an individual with eosinophilia...
  65. ncbi request reprint The alcohol dehydrogenase system
    H Jornvall
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    EXS 71:221-9. 1994
    ..The structures also correlate with functional properties and define molecular building units for the whole family...
  66. ncbi request reprint Motifer, a search tool for finding amino acid sequence patterns from nucleotide sequence databases
    H Jornvall
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 456:85-8. 1999
    ..The functions aimed at enhancing EST searches are illustrated by the 'in silico' cloning of a novel cytochrome P450 enzyme...
  67. ncbi request reprint Characterization of components of the anaerobic ribonucleotide reductase system from Escherichia coli
    R Eliasson
    Department of Biochemistry, Karolinska Institute, Stockholm, Sweden
    J Biol Chem 267:25541-7. 1992
    ..Micromolar concentrations of several chelators inhibit CTP reduction completely, suggesting the involvement of (a) transition metal(s)...
  68. ncbi request reprint Forms and functions of human SDR enzymes
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Chem Biol Interact 130:699-705. 2001
    ..This overview, prepared just before completion of the human genome project, gives the different human SDR forms and relates them to human diseases...
  69. ncbi request reprint Phosphatidate phosphatase--a key enzyme in glycerolipid biosynthesis. Studies on the yeast enzyme
    M Stark
    Department of Medical and Laboratory Sciences and Technology, Huddinge University Hospital, Sweden
    J Protein Chem 17:1-7. 1998
    ..Glycerol in the buffers could be excluded without rapid loss of enzyme activity, and Tris could be substituted for ammonium bicarbonate, while at least 0.6% sodium cholate in the buffers was essential...
  70. ncbi request reprint A novel type I receptor serine-threonine kinase predominantly expressed in the adult central nervous system
    M Ryden
    Laboratory of Molecular Neurobiology, Department of Neuroscience, Karolinska Institute, 171 77 Stockholm, Sweden
    J Biol Chem 271:30603-9. 1996
    ..The specific expression pattern of ALK-7, restricted to the postnatal central nervous system, indicates that this receptor may play an important role in the maturation and maintenance of several neuronal subpopulations...
  71. ncbi request reprint Binding of amyloid beta-peptide to mitochondrial hydroxyacyl-CoA dehydrogenase (ERAB): regulation of an SDR enzyme activity with implications for apoptosis in Alzheimer's disease
    U C Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 451:238-42. 1999
    ..The interaction of A beta with ERAB further links oxidoreductase activity with both apoptosis and amyloid toxicity...
  72. ncbi request reprint Human and rodent type 1 11beta-hydroxysteroid dehydrogenases are 7beta-hydroxycholesterol dehydrogenases involved in oxysterol metabolism
    M Hult
    Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Cell Mol Life Sci 61:992-9. 2004
    ..These results demonstrate an enzymatic origin of species differences in 7-oxysterol metabolism, establish the origin of endogenous 7beta-OH cholesterol in humans, and point to a possible involvement of 11beta-HSD1 in atherosclerosis...
  73. ncbi request reprint Purification of recombinant human apometallothionein-3 and reconstitution with zinc
    Elo Eriste
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77, Stockholm, Sweden
    Protein Expr Purif 31:161-5. 2003
    ..It showed that ZnMT-3 exists in solution as a dynamic mixture of several metalloforms, where the main metalloform is Zn(7)MT-3 and minor forms are Zn(6)MT-3 and Zn(8)MT-3...
  74. ncbi request reprint Structure and influence on stability and activity of the N-terminal propeptide part of lung surfactant protein C
    Jing Li
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS J 273:926-35. 2006
    ..These data suggest that the propeptide part of SP-Ci prevents unfolding by locking the N-terminal part of the helix, and that acidic pH results in structural disordering of the region that is proteolytically cleaved to generate SP-C...
  75. ncbi request reprint Microfluidic systems and proteomics: applications of the electrocapture technology to protein and peptide analysis
    Juan Astorga-Wells
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Anal Biochem 345:10-7. 2005
  76. ncbi request reprint Short-chain dehydrogenases/reductases (SDR): the 2002 update
    Udo Oppermann
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177, Stockholm, Sweden
    Chem Biol Interact 143:247-53. 2003
    ....
  77. ncbi request reprint Antibacterial peptides in stimulated human granulocytes: characterization of ubiquitinated histone H1A
    Yuqin Wang
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Eur J Biochem 269:512-8. 2002
    ..The tripeptide Arg-Gly-Gly corresponded uniquely to the three C-terminal residues of ubiquitin, demonstrating the presence of ubiquitinated histone H1A...
  78. ncbi request reprint Molecular effects of proinsulin C-peptide
    Jan Johansson
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 295:1035-40. 2002
    ..Combined, the available observations show that C-peptide is biologically active and suggest a molecular model for its physiological effects...
  79. doi request reprint Peptide-binding specificity of the prosurfactant protein C Brichos domain analyzed by electrospray ionization mass spectrometry
    Michael Fitzen
    Department of Medical Biochemistry and Biophysics, Division of Chemistry I, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Rapid Commun Mass Spectrom 23:3591-8. 2009
    ..The Brichos domain was also shown to bind fibril-forming peptides containing aromatic/hydrophobic residues...
  80. doi request reprint Structural features of proinsulin C-peptide oligomeric and amyloid states
    Jesper Lind
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, The Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
    FEBS J 277:3759-68. 2010
    ..This leads to the conclusion that the oligomerization of C-peptide may be relevant also at low concentrations...
  81. ncbi request reprint A novel form of neurotensin post-translationally modified by arginylation
    Elo Eriste
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 280:35089-97. 2005
    ..We have shown that SK-N-SH neuroblastoma cells express NTR1 and NTR3 but not NTR2, suggesting that the Ca2+ mobilization elicited by R-NT is via NTR1...
  82. ncbi request reprint Chemical detection of natural peptides by specific structures. Isolation of chicken galanin by monitoring for its N-terminal dipeptide, and determination of the amino acid sequence
    A Norberg
    Department of Biochemistry II, Karolinska Institute, Stockholm, Sweden
    FEBS Lett 288:151-3. 1991
    ..In addition to common substitutions at positions 16, 18, 23, 26 and 29, chicken galanin has phenylalanine at position 28, where all known mammalian galanins have leucine...
  83. ncbi request reprint Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance
    Xiaoqiu Wu
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE 171 77, Sweden
    Biochem Biophys Res Commun 313:89-96. 2004
    ..Taken together, the results underscore the importance of mRNA levels and RNA stability, but not necessarily tRNA abundance for efficient heterologous protein production in E. coli...
  84. ncbi request reprint Biochemical characterization of TASSELSEED 2, an essential plant short-chain dehydrogenase/reductase with broad spectrum activities
    Xiaoqiu Wu
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    FEBS J 274:1172-82. 2007
    ....
  85. doi request reprint Thermal unfolding of the archaeal DNA and RNA binding protein Ssh10
    Xiaoqiu Wu
    Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 373:482-7. 2008
    ..5 kcal/mol at 320 K and a DeltaG of only 6.0 kcal/mol at the optimal growth temperature of Sulfolobus...
  86. ncbi request reprint The N-terminal propeptide of lung surfactant protein C is necessary for biosynthesis and prevents unfolding of a metastable alpha-helix
    Jing Li
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Mol Biol 338:857-62. 2004
    ..Finally, in an acidic environment, SP-Ci unfolds and aggregates into amyloid fibrils like SP-C. These data suggest a direct role of the N-terminal propeptide in SP-C biosynthesis...
  87. ncbi request reprint Antimicrobial peptides in the first line defence of human colon mucosa
    Maria Tollin
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Peptides 24:523-30. 2003
    ..Combined, these results indicate that the colon mucosa is protected by a complex mixture of polypeptides, able to kill invading microbes and working in synergy as a barrier against bacterial invasion...
  88. ncbi request reprint Inhibition of nicotinoprotein (NAD+-containing) alcohol dehydrogenase by trans-4-(N,N-dimethylamino)-cinnamaldehyde binding to the active site
    Sander R Piersma
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Protein Chem 22:457-61. 2003
    ..Np-ADH is structurally related to mammalian ADH class I, and a model of np-ADH shows how the cinnamaldehyde derivative can be accommodated in the active site of the nicotinoprotein, correlating the structural and enzymological data...
  89. ncbi request reprint Cloning and characterization of the human ADH4 gene
    H von Bahr-Lindström
    Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden
    Gene 103:269-74. 1991
    ..The difference and the overall low degree of identity, 41%, of the upstream regions suggest different regulatory mechanisms for the class-I and class-II genes...
  90. ncbi request reprint Characterization of human deoxycytidine kinase. Correlation with cDNA sequences
    S Eriksson
    Medical Nobel Institute, Department of Biochemistry, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 280:363-6. 1991
    ..5 kDa dCK recently cloned. Furthermore, homology of the peptide sequences od dCK to parts of thymidine kinases and protein-tyrosine kinases are detected...
  91. ncbi request reprint Leukotriene A4 hydrolase in the human B-lymphocytic cell line Raji: indications of catalytically divergent forms of the enzyme
    B Odlander
    Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden
    Arch Biochem Biophys 287:167-74. 1991
    ..It is thus suggested, on the basis of the kinetic properties and the heat-inactivation pattern, that these enzyme preparations contained an addition form of LTA4 hydrolase...
  92. pmc Host-derived pentapeptide affecting adhesion, proliferation, and local pH in biofilm communities composed of Streptococcus and Actinomyces species
    Mirva Drobni
    Department of Odontology Cariology, Umea University, SE 901 87 Umea, and Division of Clinical Immunology, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Infect Immun 74:6293-9. 2006
    ..The present findings indicate the presence of a host-bacterium interaction in which a host peptide released by bacterial proteolysis affects key properties in biofilm formation...
  93. pmc p53 targets identified by protein expression profiling
    Rubaiyat Rahman-Roblick
    Department of Oncology Pathology, Cancer Center Karolinska, Karolinska Institutet, SE 171 76 Stockholm, Sweden
    Proc Natl Acad Sci U S A 104:5401-6. 2007
    ..Thus, this study reveals protein targets of p53 and highlights the role of transcription-independent effects for the p53-induced biological response...
  94. doi request reprint Superfamilies SDR and MDR: from early ancestry to present forms. Emergence of three lines, a Zn-metalloenzyme, and distinct variabilities
    Hans Jornvall
    Department of Medical Biochemistry and Biophysics MBB, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Biochem Biophys Res Commun 396:125-30. 2010
    ..Differences among structural and catalytic zinc ions may be relative and involve several states. Combined, the comparisons trace evolutionary properties of huge superfamilies, with partially redundant enzymes in cellular redox functions...
  95. doi request reprint Proteome patterns in uremic plasma
    Uzma Naseeb
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Blood Purif 26:561-8. 2008
    ....
  96. doi request reprint Highly active dimeric and low-activity tetrameric forms of selenium-containing rat thioredoxin reductase 1
    Olle Rengby
    Division of Biochemistry, Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 46:893-904. 2009
    ..The tetrameric forms of the protein could represent hitherto unknown regulatory states of the enzyme...
  97. pmc Proinsulin C-peptide regulates ribosomal RNA expression
    Emma Lindahl
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 285:3462-9. 2010
    ..This regulation of ribosomal RNA provides a mechanism by which C-peptide can exert transcriptional effects and implies that the peptide has growth factor activity...
  98. doi request reprint Proinsulin C-peptide interaction with protein tyrosine phosphatase 1B demonstrated with a labeling reaction
    Theres Jägerbrink
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Biochem Biophys Res Commun 387:31-5. 2009
    ..Results highlight the importance of possible phosphatase/C-peptide roles in diabetes, and the usefulness of the cross-labeling reaction also for acidic peptides like C-peptide...
  99. ncbi request reprint Formation of stable stacking zones in a flow stream for sample immobilization in microfluidic systems
    Juan Astorga-Wells
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Anal Chem 79:1057-63. 2007
    ....
  100. ncbi request reprint Identification of variant forms of the neuroendocrine peptide galanin
    Ake Norberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Rapid Commun Mass Spectrom 18:1583-91. 2004
    ..All were found to be C-terminally amidated. At least the beta-aspartyl-shifted forms could be of native occurrence. Mechanistic explanations of the mass spectrometric fragmentation patterns of the different forms are suggested...
  101. ncbi request reprint Microfluidic electrocapture for separation of peptides
    Juan Astorga-Wells
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Anal Chem 77:7131-6. 2005
    ..In addition to separation, simultaneous sample cleanup was demonstrated for peptides obtained by shotgun tryptic digestion of membrane protein extracts...