Outi Hovatta

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi request reprint Derivation of human embryonic stem cell lines, towards clinical quality
    Outi Hovatta
    Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden
    Reprod Fertil Dev 18:823-8. 2006
  2. pmc Transcriptome profiling of human pre-implantation development
    Pu Zhang
    Department of Bioscience and Nutrition, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 4:e7844. 2009
  3. pmc Challenges for the Therapeutic use of Pluripotent Stem Derived Cells
    Magda Forsberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet Stockholm, Sweden
    Front Physiol 3:19. 2012
  4. doi request reprint Global update: Sweden
    Outi Hovatta
    Karolinska Institutet, Karolinska University Hospital Huddinge, K57, SE 141 86 Stockholm, Sweden
    Regen Med 7:140-2. 2012
  5. ncbi request reprint Ovarian function and in vitro fertilization (IVF) in Turner syndrome
    Outi Hovatta
    Karolinska Institutet Karolinska University Hospital K57, SE 141 86 Stockholm, Sweden
    Pediatr Endocrinol Rev 9:713-7. 2012
  6. pmc Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods
    Susanna Narkilahti
    REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, 33520 Tampere, Finland
    Biomed Eng Online 6:11. 2007
  7. pmc A gene expression signature shared by human mature oocytes and embryonic stem cells
    Said Assou
    CHU Montpellier, Institute for Research in Biotherapy, Hopital Saint Eloi, Montpellier, F 34000 France
    BMC Genomics 10:10. 2009
  8. pmc The zebrafish transcriptome during early development
    Liselotte Vesterlund
    Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden
    BMC Dev Biol 11:30. 2011
  9. ncbi request reprint [Human cell nucleus to animal embryo. Ethically defendable British decision. but it's necessary to make the technique function]
    Outi Hovatta
    Karolinska Institutet, Karolinska Universitetssjukhuset Huddinge, Stockholm
    Lakartidningen 105:20-1. 2008
  10. doi request reprint Infectious problems associated with transplantation of cells differentiated from pluripotent stem cells
    Outi Hovatta
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital Huddinge, K57, 141 86, Stockholm, Sweden
    Semin Immunopathol 33:627-30. 2011

Detail Information

Publications106 found, 100 shown here

  1. ncbi request reprint Derivation of human embryonic stem cell lines, towards clinical quality
    Outi Hovatta
    Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden
    Reprod Fertil Dev 18:823-8. 2006
    ..Somatic cell nuclear transfer would help to overcome rejection of transplanted cells...
  2. pmc Transcriptome profiling of human pre-implantation development
    Pu Zhang
    Department of Bioscience and Nutrition, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 4:e7844. 2009
    ..Preimplantation development is a crucial step in early human development. However, the molecular basis of human preimplantation development is not well known...
  3. pmc Challenges for the Therapeutic use of Pluripotent Stem Derived Cells
    Magda Forsberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet Stockholm, Sweden
    Front Physiol 3:19. 2012
    ..After addressing these challenges, precise strategies are developed to monitor the status of the cells at different times and in case of undesired results, corresponding counteracting strategies must exist before any clinical attempt...
  4. doi request reprint Global update: Sweden
    Outi Hovatta
    Karolinska Institutet, Karolinska University Hospital Huddinge, K57, SE 141 86 Stockholm, Sweden
    Regen Med 7:140-2. 2012
    ..Successful transplantations of trachea using a tissue-engineered product with cells cultured into a scaffold have been made recently. Optimizing the stem cell type for these constructs is ongoing...
  5. ncbi request reprint Ovarian function and in vitro fertilization (IVF) in Turner syndrome
    Outi Hovatta
    Karolinska Institutet Karolinska University Hospital K57, SE 141 86 Stockholm, Sweden
    Pediatr Endocrinol Rev 9:713-7. 2012
    ..Some 30% of Turner women develop hypertension during pregnancy, but this is also common among all oocyte donation pregnancies...
  6. pmc Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods
    Susanna Narkilahti
    REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, 33520 Tampere, Finland
    Biomed Eng Online 6:11. 2007
    ..Continuous monitoring of living cell cultures can reveal more information and is faster than using laborious traditional methods such as microscopic evaluation, immunohistochemistry and flow cytometry...
  7. pmc A gene expression signature shared by human mature oocytes and embryonic stem cells
    Said Assou
    CHU Montpellier, Institute for Research in Biotherapy, Hopital Saint Eloi, Montpellier, F 34000 France
    BMC Genomics 10:10. 2009
    ..Comparison of the transcriptome of these two cell types may highlight genes that are involved in pluripotency initiation...
  8. pmc The zebrafish transcriptome during early development
    Liselotte Vesterlund
    Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden
    BMC Dev Biol 11:30. 2011
    ..In this study RNA-Seq is used to compare the transcription profiles of four early developmental stages in zebrafish (Danio rerio) on a global scale...
  9. ncbi request reprint [Human cell nucleus to animal embryo. Ethically defendable British decision. but it's necessary to make the technique function]
    Outi Hovatta
    Karolinska Institutet, Karolinska Universitetssjukhuset Huddinge, Stockholm
    Lakartidningen 105:20-1. 2008
  10. doi request reprint Infectious problems associated with transplantation of cells differentiated from pluripotent stem cells
    Outi Hovatta
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital Huddinge, K57, 141 86, Stockholm, Sweden
    Semin Immunopathol 33:627-30. 2011
    ....
  11. ncbi request reprint A large Northern European observational study of follitropin alpha filled-by-mass pre-filled pen
    Outi Hovatta
    Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Reprod Biomed Online 18:502-8. 2009
    ..Although minor differences were observed between countries, the follitropin alpha FbM pre-filled pen proved clinically effective, was generally well tolerated, and both the women and nurses/midwives found the training easy...
  12. pmc A teratocarcinoma-like human embryonic stem cell (hESC) line and four hESC lines reveal potentially oncogenic genomic changes
    Outi Hovatta
    Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 5:e10263. 2010
    ..They also point to the possibility that the acquisition of genetic changes by ESCs in culture may be used to explore certain aspects of the mechanisms regulating oncogenesis...
  13. ncbi request reprint Cost-effective approaches to in vitro fertilization: means to improve access
    O Hovatta
    Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Int J Gynaecol Obstet 94:287-91. 2006
    ..To prevent short- and long-term costs as well as possible lifelong problems, the transfer of multiple embryos should not be performed...
  14. pmc An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells
    Frida Holm
    Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, K57, Karolinska University Hospital, Huddinge, Stockholm 141 86, Sweden
    Hum Reprod 25:1271-9. 2010
    ..In addition to optimized clinical grade culture conditions, efficient clinical grade cryopreservation methods for these cells are needed. Obtaining good survival after thawing has been problematic...
  15. ncbi request reprint Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells
    Jose Inzunza
    Department of Clinical Sciences, Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Stem Cells 23:544-9. 2005
    ..We conclude that hESC lines can be successfully derived using SR medium and postnatal human fibroblasts as feeder cells. This is a step toward xeno-free conditions and facilitates the use of these cells in transplantation...
  16. ncbi request reprint Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells
    Lusine Aghajanova
    Department of Obstetrics and Gynecology, CLINTEC, Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Fertil Steril 86:1193-209. 2006
    ....
  17. doi request reprint Research resource: interactome of human embryo implantation: identification of gene expression pathways, regulation, and integrated regulatory networks
    Signe Altmäe
    Department of Clinical Science Intervention, and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, 14186 Stockholm, Sweden
    Mol Endocrinol 26:203-17. 2012
    ..We demonstrate the first systems biology approach into the complex molecular network of the implantation process in humans...
  18. ncbi request reprint Lentiviral-mediated HoxB4 expression in human embryonic stem cells initiates early hematopoiesis in a dose-dependent manner but does not promote myeloid differentiation
    Christian Unger
    Department of Medicine, M54, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Stem Cells 26:2455-66. 2008
    ..Our study highlights the importance of HoxB4 dosage and points to the need for experimental systems allowing controlled gene expression. Disclosure of potential conflicts of interest is found at the end of this article...
  19. pmc Expression analysis of the NLRP gene family suggests a role in human preimplantation development
    Pu Zhang
    Department of Bioscience and Nutrition at Novum, Karolinska Institutet, Huddinge, Sweden
    PLoS ONE 3:e2755. 2008
    ..Several NLRPs have been indicated as being involved in reproduction as well...
  20. ncbi request reprint Xeno-free culture of human pluripotent stem cells
    Rosita Bergstrom
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden
    Methods Mol Biol 767:125-36. 2011
    ....
  21. doi request reprint Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells
    Christian Unger
    Division of Obstetrics and Gynaecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden
    Hum Reprod 24:2567-81. 2009
    ..Feeder cells are frequently used for the early-stage of derivation and culture of human embryonic stem cell (hESC) lines...
  22. ncbi request reprint Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment
    Victoria Keros
    Karolinska Institute, Division of Obstetrics and Gynaecology, Department of Clinical Science, Technology and Intervention, Karolinska University Hospital, Stockholm, Sweden
    Hum Reprod 22:1384-95. 2007
    ..We wanted to find optimal methods for cryopreservation of testicular tissue from pre-pubertal boys, modifying techniques developed for fetal and adult human testicular tissue cryopreservation...
  23. doi request reprint Disturbances in the LIF pathway in the endometrium among women with unexplained infertility
    Lusine Aghajanova
    Department of Clinical Science Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Fertil Steril 91:2602-10. 2009
    ..Signaling through the LIF pathway is involved in maintenance of a receptive state of human endometrium. Impaired endometrial receptivity may be a cause of female infertility...
  24. ncbi request reprint Recombinant LH is equally effective as recombinant hCG in promoting oocyte maturation in a clinical in-vitro maturation programme: a randomized study
    Julius Hreinsson
    Karolinska Institutet, Department of Obstetrics and Gynaecology, Huddinge University Hospital, Stockholm, Sweden and The Family Federation of Finland, Kalevagatan 16, Helsinki, Finland
    Hum Reprod 18:2131-6. 2003
    ..In-vitro maturation (IVM) may prove important for subjects needing fertility preservation, and also provides information about the final stages of oocyte maturation...
  25. ncbi request reprint Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells
    Pu Zhang
    Department of Biosciences and Nutrition at Novum, Karolinska Institutet, Huddinge, Sweden
    Fertil Steril 87:677-90. 2007
    ..To identify genes that are expressed differently during final oocyte maturation and early embryonic development in humans...
  26. ncbi request reprint Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines
    Susanne Ström
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden
    Hum Reprod 22:3051-8. 2007
    ..Immunosurgery generally used for isolation of the inner cell mass (ICM) still involves animal serum and complement...
  27. ncbi request reprint Embryo transfer is equally effective at cleavage stage and blastocyst stage: a randomized prospective study
    Julius Hreinsson
    Department of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Fertility Unit K 59, Halsovagen, S 14186 Stockholm, Sweden
    Eur J Obstet Gynecol Reprod Biol 117:194-200. 2004
    ..To compare the implantation and pregnancy rates after cleavage stage embryo transfer (ET) with transfer of blastocyst-stage (days 5-6) embryos...
  28. ncbi request reprint Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants
    Victoria Keros
    Karolinska Institute, Division of Obstetrics and Gynaecology, Department of Clinical Science, Karolinska University Hospital, Huddinge, SE 141 86 Stockholm, Sweden
    Hum Reprod 20:1676-87. 2005
    ..We compared three different protocols and cryoprotectants in cryopreservation of testicular tissue...
  29. doi request reprint Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue
    Victoria Keros
    Department of Clinical Science, Technology and Intervention, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, K 57, Stockholm SE 141 86, Sweden
    Hum Reprod 24:1670-83. 2009
    ..To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing...
  30. ncbi request reprint Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor
    Heidi Koivisto
    Department of Clinical Science, Karolinska Institute, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Reprod Biomed Online 9:330-7. 2004
    ..The FCS supported the non-differentiated growth poorly. Basic fibroblast growth factor supported non-differentiated growth, the highest concentration (8 ng/ml) giving the best result, while ITS was not beneficial...
  31. doi request reprint In vivo differentiated human embryonic stem cells can acquire chromosomal aberrations more frequently than in vitro during the same period
    Marco Zucchelli
    Department of Biosciences and Nutrition, Karolinska Institutet, Novum, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Stem Cells Dev 21:3363-71. 2012
    ....
  32. pmc Clinical grade vitrification of human ovarian tissue: an ultrastructural analysis of follicles and stroma in vitrified tissue
    Mona Sheikhi
    Division of Obstetrics and Gynaecology, Department of Clinical, Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Hum Reprod 26:594-603. 2011
    ..To carry out vitrification in a clinical setting, we have developed a clinical grade closed system to avoid direct contact of ovarian tissue with liquid nitrogen...
  33. ncbi request reprint Costimulation blockade induces tolerance to HESC transplanted to the testis and induces regulatory T-cells to HESC transplanted into the heart
    Karl Henrik Grinnemo
    Department of Molecular Medicine and Surgery, Division of Cardiothoracic Surgery and Anaesthesiology, Karolinska University Hospital, S 171 76, Stockholm, Sweden
    Stem Cells 26:1850-7. 2008
    ..HESC specific regulatory T-cells developed to HESC transplanted to the heart and the success of transplantation was similar to that seen in SCID mice...
  34. ncbi request reprint Preservation of human ovarian follicles within tissue frozen by vitrification in a xeno-free closed system using only ethylene glycol as a permeating cryoprotectant
    Mona Sheikhi
    Division of Obstetrics and Gynecology, Department of Clinical, Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Fertil Steril 100:170-7.e1-2. 2013
    ..To study the preservation of follicles within ovarian tissue vitrified using only one or a combination of three permeating cryoprotectants...
  35. ncbi request reprint Fertility preservation in girls with turner syndrome: prognostic signs of the presence of ovarian follicles
    Birgit Borgström
    Department of Pediatrics, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden
    J Clin Endocrinol Metab 94:74-80. 2009
    ..Many girls with Turner syndrome have follicles in their ovaries at adolescence...
  36. doi request reprint Tissue factor and tissue factor pathway inhibitors TFPI and TFPI2 in human secretory endometrium--possible link to female infertility
    Signe Altmäe
    Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Reprod Sci 18:666-78. 2011
    ..No distinct involvement of TFPI2 in the regulation of endometrial receptivity and unexplained infertility was found...
  37. ncbi request reprint Neurotrophic factors promote cholinergic differentiation in human embryonic stem cell-derived neurons
    Mats Nilbratt
    Karolinska Institutet, Karolinska University Hospital Huddinge, Department of Neurobiology, Care Sciences and Society, Stockholm, Sweden
    J Cell Mol Med 14:1476-84. 2010
    ....
  38. pmc No relationship between embryo morphology and successful derivation of human embryonic stem cell lines
    Susanne Ström
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 5:e15329. 2010
    ..Embryo quality is important as regards the likelihood of pregnancy, but there is little information regarding likelihood of stem cell derivation...
  39. ncbi request reprint Exclusion of coding-region mutations in luteinizing hormone and follicle-stimulating hormone receptor genes as the cause of ovarian hyperstimulation syndrome
    Erja Kerkela
    Department of Biosciences, Novum, Karolinska Institutet, Huddinge, Sweden
    Fertil Steril 87:603-6. 2007
    ....
  40. doi request reprint Detection of a procoagulable state during controlled ovarian hyperstimulation for in vitro fertilization with global assays of haemostasis
    Eli Westerlund
    Division of Medicine, Department of Clinical Sciences, Danderyd Hospital, Stockholm, Sweden
    Thromb Res 130:649-53. 2012
    ..In the present study we investigated the effect of the standard IVF-stimulation protocol on coagulation and fibrinolysis as assessed by different global haemostatic assays...
  41. ncbi request reprint Benefits of 8-bromo-guanosine 3',5'-cyclic monophosphate (8-br-cGMP) in human ovarian cortical tissue culture
    Jennifer E Scott
    Karolinska Institutet, Department of Obstetrics and Gynaecology, Karolinska University Hospital Huddinge, S 141 86 Stockholm, Sweden
    Reprod Biomed Online 8:319-24. 2004
    ..In the early stages of human ovarian tissue culture, 8-br-cGMP may be a necessary component as both a growth enhancer and survival factor...
  42. ncbi request reprint Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture
    Inger B Carlsson
    Karolinska Institutet, Department of Clinical Science, Intervention and Technology Karolinska University Hospital, Stockholm, Sweden
    Reproduction 131:641-9. 2006
    ..Blocking the c-Kit receptor induces follicular atresia. The KL/c-Kit signaling system is likely to control the survival of human ovarian follicles during early follicular development...
  43. doi request reprint Luteal phase progesterone increases live birth rate after frozen embryo transfer
    Kerstin Bjuresten
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden
    Fertil Steril 95:534-7. 2011
    ..To see if progesterone support has a beneficial effect on live birth rate after frozen embryo transfer in natural cycles...
  44. ncbi request reprint Receptors for thyroid-stimulating hormone and thyroid hormones in human ovarian tissue
    Lusine Aghajanova
    Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, 14186 Stockholm, Sweden
    Reprod Biomed Online 18:337-47. 2009
    ..These data demonstrate that TSH and thyroid hormone receptors may participate in the regulation of ovarian function...
  45. doi request reprint Thyroid-stimulating hormone receptor and thyroid hormone receptors are involved in human endometrial physiology
    Lusine Aghajanova
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Fertil Steril 95:230-7, 237.e1-2. 2011
    ..To study the expression, distribution, and function of thyroid-stimulating hormone receptor (TSHR) and thyroid hormone receptors (TR) α1, α2, and β1 in human endometrium...
  46. doi request reprint Co-localization of neural cell adhesion molecule and fibroblast growth factor receptor 2 in early embryo development
    Liselotte Vesterlund
    Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
    Int J Dev Biol 55:313-9. 2011
    ..In summary these findings show a co-localization of NCAM and FGFR2 in early vertebrate development with intracellular signaling pathways present to enable a cellular response...
  47. ncbi request reprint Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
    Sergey Rodin
    Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Nat Biotechnol 28:611-5. 2010
    ..This xeno-free and feeder-free system may be useful for the development of cell lineages for therapeutic purposes...
  48. ncbi request reprint Allelic estrogen receptor 1 (ESR1) gene variants predict the outcome of ovarian stimulation in in vitro fertilization
    Signe Altmäe
    Department of Biotechnology, Institute of Molecular and Cell Biology, Estonian Genome Project, University of Tartu, Estonia, and Department of Clinical Science, Intervention and Technology, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Mol Hum Reprod 13:521-6. 2007
    ..These data suggest the variations in ESR1 gene, in addition to the age of a woman, may predict the COH outcome in IVF...
  49. ncbi request reprint Follicles are found in the ovaries of adolescent girls with Turner's syndrome
    Julius Gisli Hreinsson
    Department of Obstetrics and Gynecology, Karolinska Institutet, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    J Clin Endocrinol Metab 87:3618-23. 2002
    ....
  50. ncbi request reprint Immunogenicity of human embryonic stem cells
    Karl Henrik Grinnemo
    Division of Cardiothoracic Surgery and Anaesthesiology, Department of Molecular Medicine and Surgery, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden
    Cell Tissue Res 331:67-78. 2008
    ....
  51. ncbi request reprint Glycodelin is present in pinopodes of receptive-phase human endometrium and is associated with down-regulation of progesterone receptor B
    Anneli Stavreus-Evers
    Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Fertil Steril 85:1803-11. 2006
    ..To test the hypothesis that glycodelin is localized on pinopodes and correlates with temporal immunostaining of leukemia inhibitory factor (LIF), LIF receptor (LIFR), and progesterone receptor B (PRB)...
  52. ncbi request reprint Derivation of human skin fibroblast lines for feeder cells of human embryonic stem cells
    Christian Unger
    Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
    Curr Protoc Stem Cell Biol . 2008
    ..Here, we describe the derivation, culture, and cryopreservation procedures of research grade human skin fibroblast lines. We also describe how to make feeder layers for hESC using these fibroblasts...
  53. pmc Ovarian wedge resection restores fertility in estrogen receptor beta knockout (ERbeta-/-) mice
    Jose Inzunza
    Division of Medical Nutrition, Department of Biosciences and Nutrition, Karolinska University Hospital, Karolinska Institutet, Novum, S 141 86 Stockholm, Sweden
    Proc Natl Acad Sci U S A 104:600-5. 2007
    ..The follicles do not mature because of insufficient blood supply. This problem is overcome by stimulating neovascularization by simple wedge resection of the ovaries...
  54. doi request reprint Good manufacturing practice and clinical-grade human embryonic stem cell lines
    Christian Unger
    Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 14186 Stockholm, Sweden
    Hum Mol Genet 17:R48-53. 2008
    ..Human serum is a xeno-free component available today, but many chemically defined media are under development...
  55. pmc Differences in Gene Expression between Mouse and Human for Dynamically Regulated Genes in Early Embryo
    Elo Madissoon
    Department of Biosciences and Nutrition Center for Biosciences, Karolinska Institutet, Huddinge, Sweden
    PLoS ONE 9:e102949. 2014
    ..We also describe four cancer-testis antigen families that are also highly expressed in human embryos: PRAME, SSX, GAGE and MAGEA. ..
  56. ncbi request reprint Few instead of many: human follicle collection from follicular aspirates at oocyte retrieval
    Pu Zhang
    Department of Clinical Science, Karolinska Institutet, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Hum Reprod 17:3190-2. 2002
    ..The aim of this study was to determine if follicular aspirates obtained during oocyte retrieval for IVF were a good source of ovarian follicles for research purposes...
  57. pmc Costimulation blockade induces foxp3(+) regulatory T cells to human embryonic stem cells
    Karin Ljung
    Department of Emergency Medicine Huddinge, Karolinska University Hospital, Stockholm, Sweden
    Biores Open Access 2:455-8. 2013
    ..These results imply that costimulation blockade could be a useful treatment strategy for the induction of tolerance to hESC transplants and may down-modulate immune responses locally around the graft...
  58. ncbi request reprint Coexpression of pinopodes and leukemia inhibitory factor, as well as its receptor, in human endometrium
    Lusine Aghajanova
    Department of Clinical Science, Division of Obstetrics and Gynecology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Fertil Steril 79:808-14. 2003
    ..To determine cell-type-specific expression of leukemia inhibitory factor (LIF) and LIF receptor (LIFR) proteins relative to formation of pinopodes in human endometrial samples...
  59. ncbi request reprint Cryopreservation of follicles in human ovarian cortical tissue. Comparison of serum and human serum albumin in the cryoprotectant solutions
    Julius Hreinsson
    Karolinska Institutet, Department of Obstetrics and Gynaecology, Huddinge University Hospital and Karolinska Institutet, Clinical Research Centre, Novum, S 141 86 Stockholm, Sweden
    Hum Reprod 18:2420-8. 2003
    ..We compared human serum [20% (v/v)] and human serum albumin (HSA) (25 mg/ml) in cryoprotectant solutions containing propanediol and sucrose to evaluate whether serum-free medium could be used for this purpose...
  60. pmc In vitro effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles
    Pu Zhang
    Department of Clinical Science, Division of Obstetrics and Gynecology, Karolinska Institute, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    J Assist Reprod Genet 21:301-6. 2004
    ..To test the effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period...
  61. ncbi request reprint Human ovarian tissue cultures: extracellular matrix composition, coating density and tissue dimensions
    Jennifer E Scott
    Karolinska Institutet, Department of Obstetrics and Gynaecology, Karolinska University Hospital Huddinge, S 141 86 Stockholm, Sweden
    Reprod Biomed Online 9:287-93. 2004
    ..This technique may provide a solution to the successful recovery and growth of follicles from frozen human ovarian tissue even though it will take time and much more optimization before it can be used in clinical practice...
  62. ncbi request reprint A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells
    Outi Hovatta
    Department of Clinical Science, Karolinska Institutet, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Hum Reprod 18:1404-9. 2003
    ....
  63. ncbi request reprint The liver X receptor-{beta} is essential for maintaining cholesterol homeostasis in the testis
    Kirsten M Robertson
    Karolinska Institutet, Department of Biosciences at Novum, Huddinge 14157, Sweden
    Endocrinology 146:2519-30. 2005
    ..Although the precise molecular mechanism at this time remains unclear, our study highlights the crucial role for LXRbeta in retaining cholesterol homeostasis in Sertoli cells...
  64. ncbi request reprint Methods for cryopreservation of human ovarian tissue
    Outi Hovatta
    Karolinska Institutet, Department of Clinical Science, Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    Reprod Biomed Online 10:729-34. 2005
    ..Maturation of follicles in vitro from frozen-thawed tissue is another option that is still under development...
  65. pmc Β-amyloid 1-42 oligomers impair function of human embryonic stem cell-derived forebrain cholinergic neurons
    Linn Wicklund
    Department of Neurobiology, Care Sciences and Society, Division of Alzheimer Neurobiology, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 5:e15600. 2010
    ..We propose that it may be possible for future AD therapies to promote the maturation of functional stem cell-derived neurons by altering the brain microenvironment with trophic support and by targeting different aggregation forms of Aβ...
  66. pmc Derivation of 30 human embryonic stem cell lines--improving the quality
    Susanne Ström
    Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    In Vitro Cell Dev Biol Anim 46:337-44. 2010
    ..The lines are karyotypically normal, but culture adaptation in two lines has been observed. Our human embryonic stem cell lines are banked, and they are available for researchers...
  67. ncbi request reprint Embryo transfer by midwife or gynecologist: a prospective randomized study
    Kerstin Bjuresten
    Department of Obstetrics and Gynaecology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
    Acta Obstet Gynecol Scand 82:462-6. 2003
    ..The aim of this study was to see if a skilled IVF midwife could perform ET with similar results to a gynecologist...
  68. ncbi request reprint Human embryonic stem cells are immunogenic in allogeneic and xenogeneic settings
    Karl Henrik Grinnemo
    Department of Cardiothoracic Surgery and Anaesthesiology, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden
    Reprod Biomed Online 13:712-24. 2006
    ..It is concluded that HESC are immunologically inert and do not inhibit immune responses during direct or indirect antigen presentation, and they were acutely rejected in a xenogeneic setting...
  69. ncbi request reprint Transplantation of embryonic stem cells: possibilities and challenges
    Olle Ringden
    Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Transplantation 76:1011-2. 2003
  70. doi request reprint Clinical aspects of fertility preservation in female patients
    Kenny A Rodriguez Macias Wallberg
    Division of Obstetrics and Gynecology, Fertility Unit, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Pediatr Blood Cancer 53:254-60. 2009
    ..It is often possible to save ovarian tissue even though the first chemotherapy courses have been undergone, but many more follicles can be stored before cancer treatment...
  71. pmc Activation of neural and pluripotent stem cell signatures correlates with increased malignancy in human glioma
    Johan Holmberg
    Ludwig Institute for Cancer Research, Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 6:e18454. 2011
    ....
  72. ncbi request reprint Cryopreservation and culture of human ovarian cortical tissue containing early follicles
    Outi Hovatta
    Department of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, S 14186 Stockholm, Sweden
    Eur J Obstet Gynecol Reprod Biol 113:S50-4. 2004
    ..Maturation in vitro (IVM) of oocytes obtained by aspiration from small antral follicles is already a feasible technique resulting in pregnancies, with many healthy infants born...
  73. ncbi request reprint Cryobiology of ovarian and testicular tissue
    Outi Hovatta
    Division of Obstetrics and Gynaecology, Department of Clinical Science, Karolinska Institute, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Best Pract Res Clin Obstet Gynaecol 17:331-42. 2003
    ..Cryopreservation of testicular spermatozoa for intracytoplasmic sperm injection (ICSI) is an established method and should be performed whenever a testicular biopsy is being carried out...
  74. ncbi request reprint Feeder-free derivation of human embryonic stem-cell lines
    Outi Hovatta
    Department of Clinical Science, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    Lancet 365:1601-3. 2005
  75. ncbi request reprint Coxsackievirus and adenovirus receptor (CAR) is expressed in male germ cells and forms a complex with the differentiation factor JAM-C in mouse testis
    Momina Mirza
    Ludwig Institute for Cancer Research, Stockholm Branch, Karolinska Institute, PO Box 240, SE 17177 Stockholm, Sweden
    Exp Cell Res 312:817-30. 2006
    ..Together, these findings imply a function for CAR in male fertility. The results also suggest that CAR in spermatozoa is inaccessible to adenovirus-based gene therapy vectors, and that the risk of germ line infection therefore is low...
  76. ncbi request reprint Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures
    Roxana Nat
    Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden
    Glia 55:385-99. 2007
    ..Our system successfully generates human RG cells and could be an effective source for neuronal replacement, since RG cells predominantly generate neurons and provide them with support and guidance...
  77. ncbi request reprint Differential lipolytic regulation in human embryonic stem cell-derived adipocytes
    Vanessa van Harmelen
    Department of Medicine, M61, Karolinska University Hospital, Huddinge 141 86, Stockholm, Sweden
    Obesity (Silver Spring) 15:846-52. 2007
    ..However, determination of lipolytic response, the most important characteristic of adipocytes, has not been performed. This work was intended to study adipogenic conversion of hESCs by functional assessment of differentiation...
  78. ncbi request reprint Anti-Müllerian hormone inhibits initiation of growth of human primordial ovarian follicles in vitro
    I B Carlsson
    Division of Obstetrics and Gynaecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Hum Reprod 21:2223-7. 2006
    ..Furthermore, the ovarian follicle pool diminishes prematurely in AMH-knockout mice. In this study, we examined whether AMH plays a similar role in humans, controlling ovarian follicle growth...
  79. ncbi request reprint Gonadotrophin stimulation of non-luteinized granulosa cells increases steroid production and the expression of enzymes involved in estrogen and progesterone synthesis
    M Lindeberg
    Department of Clinical Science Investigation and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Hum Reprod 22:401-6. 2007
    ..When in vitro maturation (IVM) of the oocytes is performed, non-luteinized GCs can be collected. We have investigated how these cells respond to gonadotrophin stimulation in culture...
  80. doi request reprint Changes in von Willebrand factor and ADAMTS13 during IVF
    Eli Westerlund
    Division of Medicine, Department of Clinical Sciences, Danderyd Hospital, Stockholm, Sweden
    Blood Coagul Fibrinolysis 22:127-31. 2011
    ..This could in part explain why these patients have an increased risk of thrombotic events...
  81. pmc Birth of a healthy male after frozen thawed blastocyst transfer following intracytoplasmic injection of frozen thawed testicular spermatozoa from a man with nonmosaic Klinefelter's syndrome
    Björn Rosenlund
    Department of Obstetrics and Gynecology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
    J Assist Reprod Genet 19:149-51. 2002
    ..Testicular sperm were retrieved by percutaneous needle biopsy...
  82. ncbi request reprint Donation of embryos for stem cell research--how many couples consent?
    Kerstin Bjuresten
    Department of Obstetrics and Gynaecology, Karolinska Institute, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Hum Reprod 18:1353-5. 2003
    ..The opinions of infertile couples and their willingness to donate their supernumerary embryos for stem cell research have not been investigated earlier...
  83. ncbi request reprint Pregnancies after oocyte donation in women with ovarian failure caused by an inactivating mutation in the follicle stimulating hormone receptor
    Outi Hovatta
    Department of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, Sweden
    Hum Reprod 17:124-7. 2002
    ..An inactivating point mutation (Ala189Val) in the FSH receptor (FSHR) causes primary ovarian failure. It has not been known if FSH action is necessary during pregnancy and childbirth...
  84. ncbi request reprint Cryopreservation and culture of human primordial and primary ovarian follicles
    O Hovatta
    Department of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, Sweden
    Mol Cell Endocrinol 169:95-7. 2000
    ..The culture conditions have to be improved to get systematically early antral follicles for a second step of maturation of cumulus-oocyte-complexes...
  85. ncbi request reprint The female-to-male transsexual patient: a source of human ovarian cortical tissue for experimental use
    - Van Den Broecke R
    Department of Gynaecologic Oncology, Infertility Centre, Ghent University Hospital, Ghent, Belgium and Department of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, Sweden
    Hum Reprod 16:145-147. 2001
    ....
  86. ncbi request reprint A role for the androgen receptor in follicular atresia of estrogen receptor beta knockout mouse ovary
    Guojun Cheng
    Department of Medical Nutrition, Karolinska Institute, Novum, S 14186 Huddinge, Sweden
    Biol Reprod 66:77-84. 2002
    ....
  87. ncbi request reprint Growth differentiation factor-9 promotes the growth, development, and survival of human ovarian follicles in organ culture
    Julius Gisli Hreinsson
    Karolinska Institutet, Department of Obstetrics and Gynecology, Huddinge University Hospital, S 141 86 Huddinge, Sweden
    J Clin Endocrinol Metab 87:316-21. 2002
    ..This oocyte-derived factor may be essential for the development of somatic cells in early human follicles and useful in designing culture conditions for maturation of follicles and oocytes in vitro...
  88. ncbi request reprint A North European perspective in assisted reproduction-new legislation is coming in Finland and in Sweden
    Outi Hovatta
    Karolinska Institutet, Department of Obstetrics and Gynaecology, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Reprod Biomed Online 4:197-8. 2002
  89. ncbi request reprint Co-existence of heparin-binding epidermal growth factor-like growth factor and pinopodes in human endometrium at the time of implantation
    Anneli Stavreus-Evers
    Department of Woman and Child Health, Division of Reproductive Endocrinology, Karolinska Hospital, Stockholm, Sweden
    Mol Hum Reprod 8:765-9. 2002
    ..Furthermore, the immunohistochemical staining demonstrates that HB-EGF can be used as a marker for the implantation window...
  90. ncbi request reprint Culture and expansion of the human embryonic stem cell line HS181, evaluated in a double-color system
    Marta P Imreh
    Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
    Stem Cells Dev 13:337-43. 2004
    ..In summary, a two-color system allows analysis of colony formation and also helps to identify and follow the differentiation of cells...
  91. ncbi request reprint Stem cell transplantation--a new era in medicine?
    Outi Hovatta
    Karolinska Institutet, Department of Clinical Science, Karolinska University Hospital Huddinge, SE 141 86 Stockholm, Sweden
    Ann Med 37:466-8. 2005
  92. ncbi request reprint Surface morphology of the human endometrium. Basic and clinical aspects
    G Nikas
    Division of Obstetrics and Gynaecology, Huddinge Hospital, Karolinska Institute, Stockholm, Sweden
    Ann N Y Acad Sci 900:316-24. 2000
    ..This article recapitulates published figures of endometrial ultrastructure and presents some unpublished data from ongoing studies...
  93. ncbi request reprint Clinical outcome of treatment cycles using preimplantation genetic diagnosis for structural chromosomal abnormalities
    M Fridström
    Department of Clinical Sciences, Division of Obstetrics and Gynecology, Karolinska Institute, K 57 Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Prenat Diagn 21:781-7. 2001
    ..To explore oocyte recovery, embryo quality, the number of transferable embryos and pregnancy rate after preimplantation genetic diagnosis (PGD) in patients with structural chromosomal aberrations...
  94. ncbi request reprint Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation
    J Inzunza
    Department of Clinical Sciences, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Mol Hum Reprod 10:461-6. 2004
    ..Single cell CGH is a method that can be used for continuous analysis of the hES cell lines during cultivation, in order to detect chromosome imbalance...
  95. ncbi request reprint Pregnancies in women with Turner's syndrome
    O Hovatta
    Department of Obstetrics and Gynaecology, Karolinska Institute, Huddinge University Hospital, Sweden
    Ann Med 31:106-10. 1999
    ..Fertility counselling has become important in the treatment of girls with Turner's syndrome...
  96. ncbi request reprint Testing of nine different xeno-free culture media for human embryonic stem cell cultures
    Kristiina Rajala
    REGEA, Institute for Regenerative Medicine, University of Tampere, Tampere University Hospital, Tampere, Finland
    Hum Reprod 22:1231-8. 2007
    ..However, currently used culture conditions contain animal-derived components that bear a risk of transmitting animal pathogens and incorporation of non-human immunogenic molecules to hESC...
  97. ncbi request reprint In vitro neural differentiation of human embryonic stem cells
    Roxana Nat
    Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd, Bucharest 050474, Romania
    J Cell Mol Med 8:570-1. 2004
  98. ncbi request reprint Development and characterization of an endometrial tissue culture system
    Anneli Stavreus-Evers
    Division of Reproductive Endocrinology, Department of Woman and Child Health, Karolinska Hospital, 171 76 Stockholm, Sweden
    Reprod Biomed Online 7:243-9. 2003
    ..There was an increased sensitivity to culture after the implantation window. This new method for culture of endometrial strips in vitro has proven successful and may be used for functional studies of the human endometrium...
  99. ncbi request reprint Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
    Oluseun Adewumi
    UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
    Nat Biotechnol 25:803-16. 2007
    ..Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected...
  100. pmc A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas
    Said Assou
    Centre Hospitalier Universitaire de Montpellier, Institute for Research in Biotherapy, Hopital Saint Eloi, 80 avenue Augustin Fliche, 34295 Montpellier Cedex 5, France
    Stem Cells 25:961-73. 2007
    ..montp.inserm.fr, to provide an easy access to this public transcriptome dataset. Expression histograms comparing hESCs to a broad collection of fetal and adult tissues can be retrieved with this web tool for more than 15,000 genes...
  101. ncbi request reprint Culture conditions for human embryonic stem cells
    Heli Skottman
    REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, 33520 Tampere, Finland
    Reproduction 132:691-8. 2006
    ..It is known that culture conditions influence gene expression and, hence, probably many properties of the cells. Optimization and standardization of culture methods is needed for research as well as for clinical purposes...