Jan Holgersson

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. doi request reprint A new generation of carbohydrate-based therapeutics: recombinant mucin-type fusion proteins as versatile inhibitors of protein-carbohydrate interactions
    Anki Gustafsson
    Karolinska Institute, Karolinska University Hospital, Division of Clinical Immunology, F 79, S 141 86 Stockholm, Sweden
    Expert Opin Drug Discov 1:161-78. 2006
  2. doi request reprint Can ABO-incompatible organ transplantation pave the way for clinical xenotransplantation?
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institute, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 84:S48-50. 2007
  3. ncbi request reprint Anti-LFA-1 improves pig islet xenograft function in diabetic mice when long-term acceptance is induced by CTLA4Ig/anti-CD40L
    Makiko Kumagai-Braesch
    Department of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 83:1259-67. 2007
  4. ncbi request reprint Only a specific subset of human peripheral-blood monocytes has endothelial-like functional capacity
    Elzafir Elsheikh
    Division of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Blood 106:2347-55. 2005
  5. doi request reprint Core saccharide dependence of sialyl Lewis X biosynthesis
    Jonas Löfling
    Division of Clinical Immunology and Transfusion Medicine, F79, Karolinska Institute, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden
    Glycoconj J 26:33-40. 2009
  6. ncbi request reprint Long-term gene expression and metabolic control exerted by lentivirus-transduced pancreatic islets
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Xenotransplantation 13:195-203. 2006
  7. ncbi request reprint Anti-pig antibody adsorption efficacy of {alpha}-Gal carrying recombinant P-selectin glycoprotein ligand-1/immunoglobulin chimeras increases with core 2 {beta}1, 6-N-acetylglucosaminyltransferase expression
    Jining Liu
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, S 141 86 Stockholm, Sweden
    Glycobiology 15:571-83. 2005
  8. ncbi request reprint Porcine endothelium activated by anti-alpha-GAL antibody binding mediates increased human neutrophil adhesion under flow
    Cecilia Ehrnfelt
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Transplantation 76:1112-9. 2003
  9. ncbi request reprint Anti-Gal IgG potentiates natural killer cell migration across porcine endothelium via endothelial cell activation and increased natural killer cell motility triggered by CD16 cross-linking
    Elenor Hauzenberger
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1154-63. 2004
  10. ncbi request reprint Aberrant expression of alpha-Gal on primary human endothelium does not confer susceptibility to NK cell cytotoxicity or increased NK cell adhesion
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1185-95. 2004

Collaborators

Detail Information

Publications30

  1. doi request reprint A new generation of carbohydrate-based therapeutics: recombinant mucin-type fusion proteins as versatile inhibitors of protein-carbohydrate interactions
    Anki Gustafsson
    Karolinska Institute, Karolinska University Hospital, Division of Clinical Immunology, F 79, S 141 86 Stockholm, Sweden
    Expert Opin Drug Discov 1:161-78. 2006
    ..In particular, recombinant mucin-type proteins can serve as natural scaffolds for multivalent presentation of therapeutic carbohydrate determinants...
  2. doi request reprint Can ABO-incompatible organ transplantation pave the way for clinical xenotransplantation?
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institute, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 84:S48-50. 2007
    ..This paper discusses the molecular similarities and differences between an ABO-incompatible allograft and a porcine xenograft in terms of their interactions with the immune system...
  3. ncbi request reprint Anti-LFA-1 improves pig islet xenograft function in diabetic mice when long-term acceptance is induced by CTLA4Ig/anti-CD40L
    Makiko Kumagai-Braesch
    Department of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 83:1259-67. 2007
    ..The purpose of this study was to test whether this costimulation blockade also can induce acceptance of adult pig islets transplanted to C57BL/6 mice with streptozotocin-induced diabetes...
  4. ncbi request reprint Only a specific subset of human peripheral-blood monocytes has endothelial-like functional capacity
    Elzafir Elsheikh
    Division of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Blood 106:2347-55. 2005
    ..Our results help clarify which subpopulations may restore damaged endothelium and may participate in the maintenance of vascular homeostasis...
  5. doi request reprint Core saccharide dependence of sialyl Lewis X biosynthesis
    Jonas Löfling
    Division of Clinical Immunology and Transfusion Medicine, F79, Karolinska Institute, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden
    Glycoconj J 26:33-40. 2009
    ..Our results define the specificities of FucT-III-VII in SLe(x) biosynthesis on O-glycans with different core structures and the fine specificity of the widely used anti-SLe(x) monoclonal antibody, KM93...
  6. ncbi request reprint Long-term gene expression and metabolic control exerted by lentivirus-transduced pancreatic islets
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Xenotransplantation 13:195-203. 2006
    ..To accomplish this, efficient gene transfer methods are needed. Lentiviral vectors are transgene vehicles capable of stably transducing a variety of primary, post-mitotic cells including islets...
  7. ncbi request reprint Anti-pig antibody adsorption efficacy of {alpha}-Gal carrying recombinant P-selectin glycoprotein ligand-1/immunoglobulin chimeras increases with core 2 {beta}1, 6-N-acetylglucosaminyltransferase expression
    Jining Liu
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, S 141 86 Stockholm, Sweden
    Glycobiology 15:571-83. 2005
    ..The structural complexity of the terminal Gal-Gal expressing O-glycans with both neutral as well as sialic acid-containing structures is likely to contribute to the high adsorption efficacy...
  8. ncbi request reprint Porcine endothelium activated by anti-alpha-GAL antibody binding mediates increased human neutrophil adhesion under flow
    Cecilia Ehrnfelt
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Transplantation 76:1112-9. 2003
    ..In addition, the ability of human neutrophils to adhere to human and porcine endothelium under static and flow conditions was evaluated...
  9. ncbi request reprint Anti-Gal IgG potentiates natural killer cell migration across porcine endothelium via endothelial cell activation and increased natural killer cell motility triggered by CD16 cross-linking
    Elenor Hauzenberger
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1154-63. 2004
    ..Preformed or induced anti-alpha-Gal IgG may therefore contribute to AVR by stimulating innate immune cell infiltration of the graft...
  10. ncbi request reprint Aberrant expression of alpha-Gal on primary human endothelium does not confer susceptibility to NK cell cytotoxicity or increased NK cell adhesion
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1185-95. 2004
    ....
  11. doi request reprint Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry novel O-glycans with phosphocholine and sulfate substitutions
    Stefan Gaunitz
    Division of Clinical Immunology and Transfusion Medicine, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital at Huddinge, SE 141 86 Huddinge, Sweden
    Glycobiology 23:778-96. 2013
    ..In both cell lines, the core N-acetylgalactosamine was preferentially non-branched, but small amounts of O-glycan cores with single fucose or hexose branches were found...
  12. doi request reprint Downregulation of tissue factor (TF) by RNA interference induces apoptosis and impairs cell survival of primary endothelium and tumor cells
    Cecilia Osterholm
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Cell Tissue Res 334:93-102. 2008
    ..These findings imply that certain levels of TF are required for the maintained viability and growth of endothelium and TF-expressing tumor cells...
  13. ncbi request reprint Adult porcine islets produce MCP-1 and recruit human monocytes in vitro
    Cecilia Ehrnfelt
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Xenotransplantation 11:184-94. 2004
    ....
  14. ncbi request reprint Studies of Lewis antigens and H. pylori adhesion in CHO cell lines engineered to express Lewis b determinants
    Jonas Löfling
    Division of Clinical Immunology and Transfusion Medicine, Karolinska Institute, SE 14186 Stockholm, Sweden
    Glycobiology 18:494-501. 2008
    ..Both clones examined supported adhesion of a Le(b)-binding H. pylori strain and may thus be a useful in vitro model system for H. pylori colonization/infection studies...
  15. doi request reprint Engineering of therapeutic and diagnostic O-glycans on recombinant mucin-type immunoglobulin fusion proteins expressed in CHO cells
    Linda Lindberg
    AbSorber AB, Stockholm, Sweden
    Methods Mol Biol 988:3-17. 2013
    ..The methods used to make stable CHO cell lines secreting a mucin-type fusion protein with blood group A or B determinants following expression of up to five different cDNAs are described...
  16. ncbi request reprint Characteristics of protein-carbohydrate interactions as a basis for developing novel carbohydrate-based antirejection therapies
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institute, Karolinska University Hospital at Huddinge, Stockholm, Sweden
    Immunol Cell Biol 83:694-708. 2005
    ..Novel mucin-based adsorbers of natural anti-carbohydrate antibodies will also be described...
  17. pmc Mannosylated mucin-type immunoglobulin fusion proteins enhance antigen-specific antibody and T lymphocyte responses
    Gustaf Ahlen
    Recopharma AB, Stockholm, Sweden
    PLoS ONE 7:e46959. 2012
    ..Mannosylated mucin-type fusion proteins can be used as versatile APC-targeting molecules for vaccines and as such enhance both humoral and cellular immune responses...
  18. ncbi request reprint Quantification of blood group A and B antibodies by flow cytometry using beads carrying A or B trisaccharides
    Maria Sundbäck
    AbSorber AB, Stockholm, Sweden
    Transplantation 84:S24-6. 2007
    ....
  19. pmc The extracellular adherence protein from Staphylococcus aureus inhibits neutrophil binding to endothelial cells
    Axana Haggar
    Division of Clinical Bacteriology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Infect Immun 72:6164-7. 2004
    ..Consequently, Eap also impaired their transendothelial migration. During an S. aureus infection, Eap may thus serve to reduce inflammation by inhibiting neutrophil adhesion and extravasation...
  20. doi request reprint Is there a clinical need for a diagnostic test allowing detection of chain type-specific anti-A and anti-B?
    Linda Lindberg
    AbSorber AB and the Division of Clinical Immunology and Transfusion Medicine, Karolinska Institute, Stockholm, Sweden
    Transfusion 51:494-503. 2011
    ....
  21. ncbi request reprint Multivalent Galalpha1,3Gal-substitution makes recombinant mucin-immunoglobulins efficient absorbers of anti-pig antibodies
    Jining Liu
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Xenotransplantation 10:149-63. 2003
    ....
  22. pmc Hypoxia inducible factor-1 mediates effects of insulin on pancreatic cancer cells and disturbs host energy homeostasis
    Feng Wang
    Department of Surgery, Karolinska University Hospital at Huddinge, 141 86 Stockholm, Sweden
    Am J Pathol 170:469-77. 2007
    ..These results suggest that an interaction between insulin and HIF-1alpha helps sustain pancreatic cancer cells and disturbs host energy homeostasis...
  23. ncbi request reprint Activated porcine embryonic brain endothelial cells induce a proliferative human T-lymphocyte response
    Suchitra Sumitran-Holgersson
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, S 141 86 Stockholm, Sweden
    Cell Transplant 12:637-46. 2003
    ..Within a neural graft or the regional lymph nodes, PBMEC might stimulate human T cells via the direct pathway, and should therefore be removed from the donor tissue prior to transplantation...
  24. ncbi request reprint Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Glycobiology 16:584-93. 2006
    ..Such tools will be important for investigations on the fine carbohydrate specificity of Le(b)-binding lectins, such as Helicobacter pylori adhesins and DC-SIGN, and may also prove useful as therapeutics...
  25. ncbi request reprint Leukocyte endothelial cell interactions in pig to human organ xenograft rejection
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institutet, F79 Huddinge University Hospital AB, S 141 86 Stockholm, Sweden
    Vet Immunol Immunopathol 87:407-15. 2002
    ..Antibodies specific for pig endothelial adhesion molecules can potentially be used as species (graft)-specific immunosuppressive reagents in order to prevent cellular organ xenograft rejection...
  26. pmc Capillarization of hepatic sinusoid by liver endothelial cell-reactive autoantibodies in patients with cirrhosis and chronic hepatitis
    Bo Xu
    Division of Clinical Immunology, Gastroenterology, Transplantation Surgery, and Pathology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
    Am J Pathol 163:1275-89. 2003
    ..Thus, anti-LSEC autoAbs transform LSE into a vascular type and may therefore play an important role in the development of hepatocellular failure and portal hypertension in PBC and AIH patients...
  27. ncbi request reprint Absorption of anti-blood group A antibodies on P-selectin glycoprotein ligand-1/immunoglobulin chimeras carrying blood group A determinants: core saccharide chain specificity of the Se and H gene encoded alpha1,2 fucosyltransferases in different host cell
    Jonas C Löfling
    Division of Clinical Immunology, F79, IMP1, Karolinska Institutet, Huddinge University Hospital AB, S 141 86 Stockholm, Sweden
    Glycobiology 12:173-82. 2002
    ..In conclusion, PSGL-1/mIgG(2b), if substituted with A epitopes, was shown to be an efficient absorber of anti-blood group A antibodies and a suitable model protein for studies on protein glycosylation...
  28. ncbi request reprint Carbohydrate phenotyping of human and animal milk glycoproteins
    Anki Gustafsson
    Department of Clinical Chemistry, Sahlgrenska University Hospital, Goteborg, Sweden
    Glycoconj J 22:109-18. 2005
    ..Thus, milk from these animals or their genetically modified descendants could have a therapeutic effect by inhibiting pathogen colonization and infection...
  29. ncbi request reprint Carbohydrate-dependent inhibition of Helicobacter pylori colonization using porcine milk
    Anki Gustafsson
    Department of Surgery, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
    Glycobiology 16:1-10. 2006
    ..pylori adhesion in vitro and H. pylori colonization in vivo. Milk from certain pig breeds may have a therapeutic and/or prophylactic effect on H. pylori infection...
  30. ncbi request reprint T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components
    Shu Shun Li
    Department of Clinical Immunology, Umea University, Umea, Sweden
    Eur J Immunol 32:1069-79. 2002
    ..These results indicate that endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes...