Jan Holgersson

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi A new generation of carbohydrate-based therapeutics: recombinant mucin-type fusion proteins as versatile inhibitors of protein-carbohydrate interactions
    Anki Gustafsson
    Karolinska Institute, Karolinska University Hospital, Division of Clinical Immunology, F 79, S 141 86 Stockholm, Sweden
    Expert Opin Drug Discov 1:161-78. 2006
  2. ncbi Can ABO-incompatible organ transplantation pave the way for clinical xenotransplantation?
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institute, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 84:S48-50. 2007
  3. ncbi Anti-LFA-1 improves pig islet xenograft function in diabetic mice when long-term acceptance is induced by CTLA4Ig/anti-CD40L
    Makiko Kumagai Braesch
    Department of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 83:1259-67. 2007
  4. ncbi Only a specific subset of human peripheral-blood monocytes has endothelial-like functional capacity
    Elzafir Elsheikh
    Division of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital-Huddinge, Stockholm, Sweden
    Blood 106:2347-55. 2005
  5. ncbi Core saccharide dependence of sialyl Lewis X biosynthesis
    Jonas Löfling
    Division of Clinical Immunology and Transfusion Medicine, F79, Karolinska Institute, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden
    Glycoconj J 26:33-40. 2009
  6. ncbi Long-term gene expression and metabolic control exerted by lentivirus-transduced pancreatic islets
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Xenotransplantation 13:195-203. 2006
  7. ncbi Anti-pig antibody adsorption efficacy of {alpha}-Gal carrying recombinant P-selectin glycoprotein ligand-1/immunoglobulin chimeras increases with core 2 {beta}1, 6-N-acetylglucosaminyltransferase expression
    Jining Liu
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, S-141 86 Stockholm, Sweden
    Glycobiology 15:571-83. 2005
  8. ncbi Aberrant expression of alpha-Gal on primary human endothelium does not confer susceptibility to NK cell cytotoxicity or increased NK cell adhesion
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1185-95. 2004
  9. ncbi Porcine endothelium activated by anti-alpha-GAL antibody binding mediates increased human neutrophil adhesion under flow
    Cecilia Ehrnfelt
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Transplantation 76:1112-9. 2003
  10. ncbi Anti-Gal IgG potentiates natural killer cell migration across porcine endothelium via endothelial cell activation and increased natural killer cell motility triggered by CD16 cross-linking
    Elenor Hauzenberger
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1154-63. 2004

Collaborators

Detail Information

Publications28

  1. ncbi A new generation of carbohydrate-based therapeutics: recombinant mucin-type fusion proteins as versatile inhibitors of protein-carbohydrate interactions
    Anki Gustafsson
    Karolinska Institute, Karolinska University Hospital, Division of Clinical Immunology, F 79, S 141 86 Stockholm, Sweden
    Expert Opin Drug Discov 1:161-78. 2006
    ..In particular, recombinant mucin-type proteins can serve as natural scaffolds for multivalent presentation of therapeutic carbohydrate determinants...
  2. ncbi Can ABO-incompatible organ transplantation pave the way for clinical xenotransplantation?
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institute, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 84:S48-50. 2007
    ..This paper discusses the molecular similarities and differences between an ABO-incompatible allograft and a porcine xenograft in terms of their interactions with the immune system...
  3. ncbi Anti-LFA-1 improves pig islet xenograft function in diabetic mice when long-term acceptance is induced by CTLA4Ig/anti-CD40L
    Makiko Kumagai Braesch
    Department of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    Transplantation 83:1259-67. 2007
    ..The purpose of this study was to test whether this costimulation blockade also can induce acceptance of adult pig islets transplanted to C57BL/6 mice with streptozotocin-induced diabetes...
  4. ncbi Only a specific subset of human peripheral-blood monocytes has endothelial-like functional capacity
    Elzafir Elsheikh
    Division of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital-Huddinge, Stockholm, Sweden
    Blood 106:2347-55. 2005
    ..Our results help clarify which subpopulations may restore damaged endothelium and may participate in the maintenance of vascular homeostasis...
  5. ncbi Core saccharide dependence of sialyl Lewis X biosynthesis
    Jonas Löfling
    Division of Clinical Immunology and Transfusion Medicine, F79, Karolinska Institute, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden
    Glycoconj J 26:33-40. 2009
    ..Our results define the specificities of FucT-III-VII in SLe(x) biosynthesis on O-glycans with different core structures and the fine specificity of the widely used anti-SLe(x) monoclonal antibody, KM93...
  6. ncbi Long-term gene expression and metabolic control exerted by lentivirus-transduced pancreatic islets
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Xenotransplantation 13:195-203. 2006
    ..Lentiviral vectors will allow genetic modifications to be performed in islets before transplantation--modifications that can improve engraftment and/or prevent xenograft rejection...
  7. ncbi Anti-pig antibody adsorption efficacy of {alpha}-Gal carrying recombinant P-selectin glycoprotein ligand-1/immunoglobulin chimeras increases with core 2 {beta}1, 6-N-acetylglucosaminyltransferase expression
    Jining Liu
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, S-141 86 Stockholm, Sweden
    Glycobiology 15:571-83. 2005
    ..The structural complexity of the terminal Gal-Gal expressing O-glycans with both neutral as well as sialic acid-containing structures is likely to contribute to the high adsorption efficacy...
  8. ncbi Aberrant expression of alpha-Gal on primary human endothelium does not confer susceptibility to NK cell cytotoxicity or increased NK cell adhesion
    Zhong He
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1185-95. 2004
    ....
  9. ncbi Porcine endothelium activated by anti-alpha-GAL antibody binding mediates increased human neutrophil adhesion under flow
    Cecilia Ehrnfelt
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Transplantation 76:1112-9. 2003
    ..In addition, the ability of human neutrophils to adhere to human and porcine endothelium under static and flow conditions was evaluated...
  10. ncbi Anti-Gal IgG potentiates natural killer cell migration across porcine endothelium via endothelial cell activation and increased natural killer cell motility triggered by CD16 cross-linking
    Elenor Hauzenberger
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Eur J Immunol 34:1154-63. 2004
    ..Preformed or induced anti-alpha-Gal IgG may therefore contribute to AVR by stimulating innate immune cell infiltration of the graft...
  11. ncbi Downregulation of tissue factor (TF) by RNA interference induces apoptosis and impairs cell survival of primary endothelium and tumor cells
    Cecilia Osterholm
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Cell Tissue Res 334:93-102. 2008
    ..These findings imply that certain levels of TF are required for the maintained viability and growth of endothelium and TF-expressing tumor cells...
  12. ncbi Adult porcine islets produce MCP-1 and recruit human monocytes in vitro
    Cecilia Ehrnfelt
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Xenotransplantation 11:184-94. 2004
    ....
  13. ncbi Characteristics of protein-carbohydrate interactions as a basis for developing novel carbohydrate-based antirejection therapies
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institute, Karolinska University Hospital at Huddinge, Stockholm, Sweden
    Immunol Cell Biol 83:694-708. 2005
    ..Novel mucin-based adsorbers of natural anti-carbohydrate antibodies will also be described...
  14. ncbi Studies of Lewis antigens and H. pylori adhesion in CHO cell lines engineered to express Lewis b determinants
    Jonas Löfling
    Division of Clinical Immunology and Transfusion Medicine, Karolinska Institute, SE 14186 Stockholm, Sweden
    Glycobiology 18:494-501. 2008
    ..Both clones examined supported adhesion of a Le(b)-binding H. pylori strain and may thus be a useful in vitro model system for H. pylori colonization/infection studies...
  15. ncbi Quantification of blood group A and B antibodies by flow cytometry using beads carrying A or B trisaccharides
    Maria Sundbäck
    AbSorber AB, Stockholm, Sweden
    Transplantation 84:S24-6. 2007
    ....
  16. ncbi Is there a clinical need for a diagnostic test allowing detection of chain type-specific anti-A and anti-B?
    Linda Lindberg
    AbSorber AB and the Division of Clinical Immunology and Transfusion Medicine, Karolinska Institute, Stockholm, Sweden
    Transfusion 51:494-503. 2011
    ....
  17. ncbi Activated porcine embryonic brain endothelial cells induce a proliferative human T-lymphocyte response
    Suchitra Sumitran-Holgersson
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, S-141 86 Stockholm, Sweden
    Cell Transplant 12:637-46. 2003
    ..Within a neural graft or the regional lymph nodes, PBMEC might stimulate human T cells via the direct pathway, and should therefore be removed from the donor tissue prior to transplantation...
  18. ncbi The extracellular adherence protein from Staphylococcus aureus inhibits neutrophil binding to endothelial cells
    Axana Haggar
    Division of Clinical Bacteriology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Infect Immun 72:6164-7. 2004
    ..Consequently, Eap also impaired their transendothelial migration. During an S. aureus infection, Eap may thus serve to reduce inflammation by inhibiting neutrophil adhesion and extravasation...
  19. ncbi Multivalent Galalpha1,3Gal-substitution makes recombinant mucin-immunoglobulins efficient absorbers of anti-pig antibodies
    Jining Liu
    Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital AB, Stockholm, Sweden
    Xenotransplantation 10:149-63. 2003
    ....
  20. ncbi Hypoxia inducible factor-1 mediates effects of insulin on pancreatic cancer cells and disturbs host energy homeostasis
    Feng Wang
    Department of Surgery, Karolinska University Hospital at Huddinge, 141 86 Stockholm, Sweden
    Am J Pathol 170:469-77. 2007
    ..These results suggest that an interaction between insulin and HIF-1alpha helps sustain pancreatic cancer cells and disturbs host energy homeostasis...
  21. ncbi Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S-141 86 Stockholm, Sweden
    Glycobiology 16:584-93. 2006
    ..Such tools will be important for investigations on the fine carbohydrate specificity of Le(b)-binding lectins, such as Helicobacter pylori adhesins and DC-SIGN, and may also prove useful as therapeutics...
  22. ncbi Engineering of therapeutic and diagnostic O-glycans on recombinant mucin-type immunoglobulin fusion proteins expressed in CHO cells
    Linda Lindberg
    AbSorber AB, Stockholm, Sweden
    Methods Mol Biol 988:3-17. 2013
    ..The methods used to make stable CHO cell lines secreting a mucin-type fusion protein with blood group A or B determinants following expression of up to five different cDNAs are described...
  23. ncbi Leukocyte endothelial cell interactions in pig to human organ xenograft rejection
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institutet, F79 Huddinge University Hospital AB, S 141 86 Stockholm, Sweden
    Vet Immunol Immunopathol 87:407-15. 2002
    ..Antibodies specific for pig endothelial adhesion molecules can potentially be used as species (graft)-specific immunosuppressive reagents in order to prevent cellular organ xenograft rejection...
  24. ncbi Capillarization of hepatic sinusoid by liver endothelial cell-reactive autoantibodies in patients with cirrhosis and chronic hepatitis
    Bo Xu
    Division of Clinical Immunology, Gastroenterology, Transplantation Surgery, and Pathology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
    Am J Pathol 163:1275-89. 2003
    ..Thus, anti-LSEC autoAbs transform LSE into a vascular type and may therefore play an important role in the development of hepatocellular failure and portal hypertension in PBC and AIH patients...
  25. ncbi Absorption of anti-blood group A antibodies on P-selectin glycoprotein ligand-1/immunoglobulin chimeras carrying blood group A determinants: core saccharide chain specificity of the Se and H gene encoded alpha1,2 fucosyltransferases in different host cell
    Jonas C Löfling
    Division of Clinical Immunology, F79, IMP1, Karolinska Institutet, Huddinge University Hospital AB, S 141 86 Stockholm, Sweden
    Glycobiology 12:173-82. 2002
    ..In conclusion, PSGL-1/mIgG(2b), if substituted with A epitopes, was shown to be an efficient absorber of anti-blood group A antibodies and a suitable model protein for studies on protein glycosylation...
  26. ncbi Carbohydrate phenotyping of human and animal milk glycoproteins
    Anki Gustafsson
    Department of Clinical Chemistry, Sahlgrenska University Hospital, Goteborg, Sweden
    Glycoconj J 22:109-18. 2005
    ..Thus, milk from these animals or their genetically modified descendants could have a therapeutic effect by inhibiting pathogen colonization and infection...
  27. ncbi Carbohydrate-dependent inhibition of Helicobacter pylori colonization using porcine milk
    Anki Gustafsson
    Department of Surgery, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
    Glycobiology 16:1-10. 2006
    ..pylori adhesion in vitro and H. pylori colonization in vivo. Milk from certain pig breeds may have a therapeutic and/or prophylactic effect on H. pylori infection...
  28. ncbi T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components
    Shu Shun Li
    Department of Clinical Immunology, Umea University, Umea, Sweden
    Eur J Immunol 32:1069-79. 2002
    ..These results indicate that endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes...