Research Topics
| Said EshaghiSummaryAffiliation: Karolinska Institutet Country: Sweden Publications
| Collaborators
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Detail Information
Publications
Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolutionSaid Eshaghi
Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
Science 313:354-7. 2006....- Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthaseDaniel Martinez Molina
Division of Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
Nature 448:613-6. 2007.... 
Catalysis within the lipid bilayer-structure and mechanism of the MAPEG family of integral membrane proteinsDaniel Martinez Molina
Department of Medical Biochemistry and Biophysics, Division of Biophysics, Karolinska Institutet, Stockholm, Sweden
Curr Opin Struct Biol 18:442-9. 2008..Structure-based sequence alignments of human MAPEG members support the notion that the glutathione binding site is highly conserved among MAPEG enzymes and that they use a similar mechanism for glutathione activation...- High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastorisAnders Wetterholm
Department of Medical Biochemistry and Biophysics, Division for Chemistry 2, Karolinska Institutet, S 171 77 Stockholm, Sweden
Protein Expr Purif 60:1-6. 2008..Hence, our approach holds promise for solving the structure of rat LTC4S and other members of the MAPEG family of integral membrane proteins... - Expression and purification of the recombinant membrane protein YidC: a case study for increased stability and solubilityDaniel Martinez Molina
Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
Protein Expr Purif 62:49-52. 2008..After optimization we could obtain several milligrams of purified YidC that could be easily prepared at high concentrations and that was stable for weeks at +4 degrees C. The isolated protein is thus well suited for structural studies... - High-throughput expression and detergent screening of integral membrane proteinsSaid Eshaghi
Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Instiutet, Stockholm, Sweden
Methods Mol Biol 498:265-71. 2009..g. salt and pH. The method requires standard laboratory equipments, but can also be automated... - A simple strategy towards membrane protein purification and crystallizationDamian Niegowski
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
Int J Biol Macromol 39:83-7. 2006.... - Exploring the activity of tobacco etch virus protease in detergent solutionsAnna Karin Lundbäck
Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
Anal Biochem 382:69-71. 2008..These observations show that, contrary to an earlier report, detergents do not inhibit the enzymatic activity of the TEV protease... - Crystal structure of a novel Thermotoga maritima enzyme (TM1112) from the cupin family at 1.83 A resolutionDaniel McMullan
The Joint Center for Structural Genomics, San Diego, California, USA
Proteins 56:615-8. 2004 - Crystal structure of a zinc-containing glycerol dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A resolutionLinda S Brinen
The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
Proteins 50:371-4. 2003 - Crystal structure of uronate isomerase (TM0064) from Thermotoga maritima at 2.85 A resolutionRobert Schwarzenbacher
Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
Proteins 53:142-5. 2003 - Crystal structure of an iron-containing 1,3-propanediol dehydrogenase (TM0920) from Thermotoga maritima at 1.3 A resolutionRobert Schwarzenbacher
The Joint Center for Structural Genomics, California 92037, USA
Proteins 54:174-7. 2004 - Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 A resolutionAndreas Heine
The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
Proteins 56:387-91. 2004 
Engineering membrane protein overproduction in Escherichia coliDaniel Martinez Molina
Department of Biochemistry and Biophysics, Stockholm University, S 106 91 Stockholm, Sweden
Protein Sci 17:673-80. 2008..In only one cycle of directed evolution were significant improvements of membrane protein yield obtained for five out of nine proteins. In one case, the yield of detergent-solubilized membrane protein was increased 40-fold...- The major peptidyl-prolyl isomerase activity in thylakoid lumen of plant chloroplasts belongs to a novel cyclophilin TLP20Anna Edvardsson
Division of Cell Biology, , , Sweden
FEBS Lett 542:137-41. 2003..TLP20 is suggested to be the major PPIase and protein folding catalyst in the thylakoid lumen of plant chloroplasts... - Subsequent events to GTP binding by the plant PsbO protein: structural changes, GTP hydrolysis and dissociation from the photosystem II complexBjörn Lundin
Division of Cell Biology, Linkoping University, SE 581 85 Linkoping, Sweden
Biochim Biophys Acta 1767:500-8. 2007..We propose the occurrence in higher plants of a PsbO-mediated GTPase activity associated with PSII, which has consequences for the function of the oxygen-evolving complex and D1 protein turnover... - Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolutionPeter Kuhn
The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
Proteins 49:142-5. 2002