Said Eshaghi

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi request reprint Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolution
    Said Eshaghi
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Science 313:354-7. 2006
  2. ncbi request reprint Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase
    Daniel Martinez Molina
    Division of Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Nature 448:613-6. 2007
  3. doi request reprint Catalysis within the lipid bilayer-structure and mechanism of the MAPEG family of integral membrane proteins
    Daniel Martinez Molina
    Department of Medical Biochemistry and Biophysics, Division of Biophysics, Karolinska Institutet, Stockholm, Sweden
    Curr Opin Struct Biol 18:442-9. 2008
  4. doi request reprint High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris
    Anders Wetterholm
    Department of Medical Biochemistry and Biophysics, Division for Chemistry 2, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Protein Expr Purif 60:1-6. 2008
  5. doi request reprint Expression and purification of the recombinant membrane protein YidC: a case study for increased stability and solubility
    Daniel Martinez Molina
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Protein Expr Purif 62:49-52. 2008
  6. doi request reprint High-throughput expression and detergent screening of integral membrane proteins
    Said Eshaghi
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Instiutet, Stockholm, Sweden
    Methods Mol Biol 498:265-71. 2009
  7. ncbi request reprint A simple strategy towards membrane protein purification and crystallization
    Damian Niegowski
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Int J Biol Macromol 39:83-7. 2006
  8. doi request reprint Exploring the activity of tobacco etch virus protease in detergent solutions
    Anna Karin Lundbäck
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Anal Biochem 382:69-71. 2008
  9. ncbi request reprint Crystal structure of a novel Thermotoga maritima enzyme (TM1112) from the cupin family at 1.83 A resolution
    Daniel McMullan
    The Joint Center for Structural Genomics, San Diego, California, USA
    Proteins 56:615-8. 2004
  10. ncbi request reprint Crystal structure of a zinc-containing glycerol dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A resolution
    Linda S Brinen
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 50:371-4. 2003

Collaborators

  • Pär Nordlund
  • Jesper Z Haeggstrom
  • Scott A Lesley
  • Chittibabu Guda
  • Glen Spraggon
  • Robert Schwarzenbacher
  • Andreas Kreusch
  • Jaume M Canaves
  • S S Taylor
  • Irimpan I Mathews
  • Jim Barber
  • Daniel Martinez Molina
  • Daniel McMullan
  • Damian Niegowski
  • Linda S Brinen
  • Peter Kuhn
  • Anna Karin Lundbäck
  • Jie Ouyang
  • Slawomir K Grzechnik
  • Heath E Klock
  • Raymond C Stevens
  • Guenter Wolf
  • Timothy M McPhillips
  • Andrew Morse
  • Carina Grittini
  • Ashley M Deacon
  • Keith O Hodgson
  • Adam Godzik
  • Kin Moy
  • Bill West
  • Alyssa Robb
  • Xiaoping Dai
  • Juli Vincent
  • Cathy Karlak
  • Henry van den Bedem
  • Ross Floyd
  • Ian A Wilson
  • John Wooley
  • Jeff Velasquez
  • Xianhong Wang
  • Lukasz Jaroszewski
  • Eric Koesema
  • Anders Wetterholm
  • Mitchell D Miller
  • Mark A Miller
  • Thomas L Selby
  • Marc A Elsliger
  • Kevin Rodrigues
  • Björn Lundin
  • Bertil Andersson
  • Rebecca Page
  • Andreas Heine
  • Frank von Delft
  • John S Kovarik
  • Anna Edvardsson
  • Hans Hebert
  • Marina Ignatushchenko Sabet
  • Helena Berglund
  • Susanne van den Berg
  • Tobias Cornvik
  • Andreas Kohl
  • Cornelia Spetea
  • Goran Samuelsson
  • Tove Hammarberg
  • Tatiana Shutova
  • Andrew A McCarthy
  • Sophie Thuswaldner
  • Eva Ohlson
  • Marie Hedrén
  • Eric Hampton
  • Qingping Xu
  • Tanya Biorac
  • Marc Andre Elsliger
  • Eileen Ambing
  • Mike DiDonato
  • Polat Abdubek
  • Inna Levin
  • Kevin Quijano
  • Ron Reyes
  • Eric Sims
  • Fred Rezezadeh
  • Alexander V Vener
  • John M Kovarik
  • Mitchell Miller

Detail Information

Publications17

  1. ncbi request reprint Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolution
    Said Eshaghi
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Science 313:354-7. 2006
    ....
  2. ncbi request reprint Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase
    Daniel Martinez Molina
    Division of Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Nature 448:613-6. 2007
    ....
  3. doi request reprint Catalysis within the lipid bilayer-structure and mechanism of the MAPEG family of integral membrane proteins
    Daniel Martinez Molina
    Department of Medical Biochemistry and Biophysics, Division of Biophysics, Karolinska Institutet, Stockholm, Sweden
    Curr Opin Struct Biol 18:442-9. 2008
    ..Structure-based sequence alignments of human MAPEG members support the notion that the glutathione binding site is highly conserved among MAPEG enzymes and that they use a similar mechanism for glutathione activation...
  4. doi request reprint High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris
    Anders Wetterholm
    Department of Medical Biochemistry and Biophysics, Division for Chemistry 2, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Protein Expr Purif 60:1-6. 2008
    ..Hence, our approach holds promise for solving the structure of rat LTC4S and other members of the MAPEG family of integral membrane proteins...
  5. doi request reprint Expression and purification of the recombinant membrane protein YidC: a case study for increased stability and solubility
    Daniel Martinez Molina
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Protein Expr Purif 62:49-52. 2008
    ..After optimization we could obtain several milligrams of purified YidC that could be easily prepared at high concentrations and that was stable for weeks at +4 degrees C. The isolated protein is thus well suited for structural studies...
  6. doi request reprint High-throughput expression and detergent screening of integral membrane proteins
    Said Eshaghi
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Instiutet, Stockholm, Sweden
    Methods Mol Biol 498:265-71. 2009
    ..g. salt and pH. The method requires standard laboratory equipments, but can also be automated...
  7. ncbi request reprint A simple strategy towards membrane protein purification and crystallization
    Damian Niegowski
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Int J Biol Macromol 39:83-7. 2006
    ....
  8. doi request reprint Exploring the activity of tobacco etch virus protease in detergent solutions
    Anna Karin Lundbäck
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Anal Biochem 382:69-71. 2008
    ..These observations show that, contrary to an earlier report, detergents do not inhibit the enzymatic activity of the TEV protease...
  9. ncbi request reprint Crystal structure of a novel Thermotoga maritima enzyme (TM1112) from the cupin family at 1.83 A resolution
    Daniel McMullan
    The Joint Center for Structural Genomics, San Diego, California, USA
    Proteins 56:615-8. 2004
  10. ncbi request reprint Crystal structure of a zinc-containing glycerol dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A resolution
    Linda S Brinen
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 50:371-4. 2003
  11. ncbi request reprint Crystal structure of uronate isomerase (TM0064) from Thermotoga maritima at 2.85 A resolution
    Robert Schwarzenbacher
    Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 53:142-5. 2003
  12. ncbi request reprint Crystal structure of an iron-containing 1,3-propanediol dehydrogenase (TM0920) from Thermotoga maritima at 1.3 A resolution
    Robert Schwarzenbacher
    The Joint Center for Structural Genomics, California 92037, USA
    Proteins 54:174-7. 2004
  13. ncbi request reprint Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 A resolution
    Andreas Heine
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 56:387-91. 2004
  14. pmc Engineering membrane protein overproduction in Escherichia coli
    Daniel Martinez Molina
    Department of Biochemistry and Biophysics, Stockholm University, S 106 91 Stockholm, Sweden
    Protein Sci 17:673-80. 2008
    ..In only one cycle of directed evolution were significant improvements of membrane protein yield obtained for five out of nine proteins. In one case, the yield of detergent-solubilized membrane protein was increased 40-fold...
  15. ncbi request reprint The major peptidyl-prolyl isomerase activity in thylakoid lumen of plant chloroplasts belongs to a novel cyclophilin TLP20
    Anna Edvardsson
    Division of Cell Biology, Linkoping University, SE 581 85 Linkoping, Sweden
    FEBS Lett 542:137-41. 2003
    ..TLP20 is suggested to be the major PPIase and protein folding catalyst in the thylakoid lumen of plant chloroplasts...
  16. ncbi request reprint Subsequent events to GTP binding by the plant PsbO protein: structural changes, GTP hydrolysis and dissociation from the photosystem II complex
    Björn Lundin
    Division of Cell Biology, Linkoping University, SE 581 85 Linkoping, Sweden
    Biochim Biophys Acta 1767:500-8. 2007
    ..We propose the occurrence in higher plants of a PsbO-mediated GTPase activity associated with PSII, which has consequences for the function of the oxygen-evolving complex and D1 protein turnover...
  17. ncbi request reprint Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolution
    Peter Kuhn
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 49:142-5. 2002