I Bjorkhem

Summary

Affiliation: Karolinska University Hospital
Country: Sweden

Publications

  1. doi request reprint Oxysterols and Parkinson's disease: evidence that levels of 24S-hydroxycholesterol in cerebrospinal fluid correlates with the duration of the disease
    Ingemar Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden Electronic address
    Neurosci Lett 555:102-5. 2013
  2. doi request reprint Cerebrotendinous xanthomatosis
    Ingemar Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden
    Curr Opin Lipidol 24:283-7. 2013
  3. doi request reprint Five decades with oxysterols
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden
    Biochimie 95:448-54. 2013
  4. pmc Effect of CAR activation on selected metabolic pathways in normal and hyperlipidemic mouse livers
    Tadeja Rezen
    Center for Functional Genomics and Bio Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, SI 1000 Ljubljana, Slovenia
    BMC Genomics 10:384. 2009
  5. pmc High levels of 15-oxygenated steroids in circulation of patients with multiple sclerosis: fact or fiction?
    I Bjorkhem
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska University Hospital Huddinge, Karolinska Institutet, Stockholm, Sweden
    J Lipid Res 52:170-4. 2011
  6. doi request reprint Oxysterols and neurodegenerative diseases
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Mol Aspects Med 30:171-9. 2009
  7. pmc Are side-chain oxidized oxysterols regulators also in vivo?
    Ingemar Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    J Lipid Res 50:S213-8. 2009
  8. ncbi request reprint Crossing the barrier: oxysterols as cholesterol transporters and metabolic modulators in the brain
    I Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska University Hospital, Huddinge, Sweden
    J Intern Med 260:493-508. 2006
  9. pmc Genetic connections between neurological disorders and cholesterol metabolism
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    J Lipid Res 51:2489-503. 2010
  10. doi request reprint Cerebrotendinous xanthomatosis: an inborn error in bile acid synthesis with defined mutations but still a challenge
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, Karolinska University Hospital Huddinge, S 14186 Huddinge, Sweden
    Biochem Biophys Res Commun 396:46-9. 2010

Collaborators

Detail Information

Publications38

  1. doi request reprint Oxysterols and Parkinson's disease: evidence that levels of 24S-hydroxycholesterol in cerebrospinal fluid correlates with the duration of the disease
    Ingemar Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden Electronic address
    Neurosci Lett 555:102-5. 2013
    ..There was no correlation between levels of 27-OH in CSF and duration of the disease. These data indicates that oxysterol levels in CSF may be of value to follow disease progression. ..
  2. doi request reprint Cerebrotendinous xanthomatosis
    Ingemar Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden
    Curr Opin Lipidol 24:283-7. 2013
    ..The mechanism behind the accumulation of cholestanol in the brain was recently clarified and a role of 27-hydroxycholesterol as a regulator of brain cholesterol homeostasis has been established...
  3. doi request reprint Five decades with oxysterols
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden
    Biochimie 95:448-54. 2013
    ..This overexpression had however only a very modest effect on cholesterol turnover. We concluded that oxysterols are not the master regulators of cholesterol homeostasis in vivo suggested previously...
  4. pmc Effect of CAR activation on selected metabolic pathways in normal and hyperlipidemic mouse livers
    Tadeja Rezen
    Center for Functional Genomics and Bio Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, SI 1000 Ljubljana, Slovenia
    BMC Genomics 10:384. 2009
    ..We therefore used 1,4-Bis [2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) to study the effect of CAR activation on mouse hepatic transcriptome and lipid metabolome under conditions of diet-induced hyperlipidemia...
  5. pmc High levels of 15-oxygenated steroids in circulation of patients with multiple sclerosis: fact or fiction?
    I Bjorkhem
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska University Hospital Huddinge, Karolinska Institutet, Stockholm, Sweden
    J Lipid Res 52:170-4. 2011
    ..We conclude that if present there are trace amounts only of the above 15-oxygenated steroids in human circulation and that the role of such oxysterols as pathogenetic factors and biomarkers must be reconsidered...
  6. doi request reprint Oxysterols and neurodegenerative diseases
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    Mol Aspects Med 30:171-9. 2009
    ..Lastly, we discuss the use of oxysterols as diagnostic markers in neurodegenerative disease...
  7. pmc Are side-chain oxidized oxysterols regulators also in vivo?
    Ingemar Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    J Lipid Res 50:S213-8. 2009
    ..The new findings will be critically reviewed here...
  8. ncbi request reprint Crossing the barrier: oxysterols as cholesterol transporters and metabolic modulators in the brain
    I Bjorkhem
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska University Hospital, Huddinge, Sweden
    J Intern Med 260:493-508. 2006
    ..An overview of the above studies is presented and the possibility that the cholesterol 24S-hydroxylase in the brain may be important for memory and learning and that it may be a new drug target is discussed...
  9. pmc Genetic connections between neurological disorders and cholesterol metabolism
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    J Lipid Res 51:2489-503. 2010
    ..In the present review, we discuss the links between genetic disturbances in cholesterol metabolism and the above neurological disorders...
  10. doi request reprint Cerebrotendinous xanthomatosis: an inborn error in bile acid synthesis with defined mutations but still a challenge
    Ingemar Bjorkhem
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, Karolinska University Hospital Huddinge, S 14186 Huddinge, Sweden
    Biochem Biophys Res Commun 396:46-9. 2010
    ..The relationship between the accumulation of cholestanol and the development of cholesterol-rich xanthomas has however not been clarified and a suitable animal model is still lacking...
  11. ncbi request reprint Studies on LXR- and FXR-mediated effects on cholesterol homeostasis in normal and cholic acid-depleted mice
    J Wang
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    J Lipid Res 47:421-30. 2006
    ..Interaction between FXR- and LXR-mediated stimuli might also regulate expression of liver ABCG5/G8...
  12. ncbi request reprint Oxysterols in the circulation of patients with the Smith-Lemli-Opitz syndrome: abnormal levels of 24S- and 27-hydroxycholesterol
    I Bjorkhem
    Division of Clinical Chemistry, Huddinge University Hospital, Sweden
    J Lipid Res 42:366-71. 2001
    ..Lütjohann, S. von Bahr, I. Pikuleva, A. Babiker, and U. Diczfaulsy. Oxysterols in the circulation of patients with the Smith-Lemli-Opitz syndrome: abnormal levels of 24S- and 27-hydroxycholesterol. J. Lipid Res. 2001. 42: 366--371...
  13. pmc Hepatic cholesterol metabolism and resistance to dietary cholesterol in LXRbeta-deficient mice
    S Alberti
    Department of Biosciences at Novum, Karolinska Institutet, Huddinge, Sweden
    J Clin Invest 107:565-73. 2001
    ..Thus, our data indicate that LXRbeta has no complete overlapping function compared with LXRalpha in the liver...
  14. ncbi request reprint Sterol 27-hydroxylase deficiency: a rare cause of xanthomas in normocholesterolemic humans
    I Bjorkhem
    Division of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, SE 141 86 Huddinge, Sweden
    Trends Endocrinol Metab 11:180-3. 2000
    ..Disruption of the gene encoding sterol 27-hydroxylase in mice does not have the same metabolic consequences as in humans...
  15. ncbi request reprint Bile acid synthesis in cultured human hepatocytes: support for an alternative biosynthetic pathway to cholic acid
    M Axelson
    Department of Clinical Chemistry, Karolinska Hospital, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden
    Hepatology 31:1305-12. 2000
    ..These results suggest that the observed intermediates reflect an alternative biosynthetic pathway to CA, which may be quantitatively significant in the cells...
  16. doi request reprint Enhanced production of 24S-hydroxycholesterol is not sufficient to drive liver X receptor target genes in vivo
    M Shafaati
    Divisions of Clinical Chemistry and Clinical Research Center, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, Huddinge, Sweden
    J Intern Med 270:377-87. 2011
    ..24S-OHC is one of the most efficient endogenous LXR agonists known and is present in the brain and in the circulation at relatively high levels...
  17. ncbi request reprint Cerebrotendinous xanthomatosis: molecular characterization of two Scandinavian sisters
    E Rystedt
    Division of Clinical Chemistry, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden
    J Intern Med 252:259-64. 2002
    ..The mutation has also previously been reported in a Japanese family. This is the second example of a CTX-causing mutation that has been recognized in more than one population...
  18. ncbi request reprint On the turnover of brain cholesterol in patients with Alzheimer's disease. Abnormal induction of the cholesterol-catabolic enzyme CYP46 in glial cells
    N Bogdanovic
    Division of Geriatric Medicine, Karolinska Institutet, Huddinge University Hospital, Huddinge, Sweden
    Neurosci Lett 314:45-8. 2001
    ..The dynamic changes in the mechanisms for cholesterol efflux from the brain are of interest in relation to the link between brain cholesterol and amyloid beta-protein in AD...
  19. ncbi request reprint From brain to bile. Evidence that conjugation and omega-hydroxylation are important for elimination of 24S-hydroxycholesterol (cerebrosterol) in humans
    I Bjorkhem
    Division of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, S 141 86 Huddinge, Sweden
    J Biol Chem 276:37004-10. 2001
    ....
  20. ncbi request reprint Correlation between serum levels of some cholesterol precursors and activity of HMG-CoA reductase in human liver
    I Bjorkhem
    Department of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    J Lipid Res 28:1137-43. 1987
    ..The activity of the hepatic microsomal HMG-CoA reductase was measured in liver biopsies taken in connection with the operation.(ABSTRACT TRUNCATED AT 250 WORDS)..
  21. ncbi request reprint Polymorphism in the coding part of the sterol 12alpha-hydroxylase gene does not explain the marked differences in the ratio of cholic acid and chenodeoxycholic acid in human bile
    A Abrahamsson
    Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska Institute, Stockholm
    Scand J Clin Lab Invest 65:595-600. 2005
    ..The aim of this study was to investigate whether this marked difference could be due to a genetic polymorphism in the gene of the CYP8B1...
  22. ncbi request reprint Oxysterols and Alzheimer's disease
    I Bjorkhem
    Division of Clinical Chemistry, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
    Acta Neurol Scand Suppl 185:43-9. 2006
    ..Current knowledge about the role of the two oxysterols for cholesterol homeostasis in the brain as well as their diagnostic potential are reviewed...
  23. ncbi request reprint Unique patient with cerebrotendinous xanthomatosis. Evidence for presence of a defect in a gene that is not identical to sterol 27-hydroxylase
    M Hansson
    Division of Clinical Chemistry, Karolinska University Hospital, Huddinge, Sweden
    J Intern Med 261:504-10. 2007
    ..It is concluded that the patient is heterozygous for two mutations affecting one allele of the CYP27A1 gene and with at least one additional yet undefined gene that is of critical importance for the activity of sterol 27-hydroxylase...
  24. ncbi request reprint Characterization of enzymes involved in formation of ethyl esters of long-chain fatty acids in humans
    M A Diczfalusy
    Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    J Lipid Res 42:1025-32. 2001
    ..A very low rate of FAEE hydrolysis was detected in heart and adipose tissue, which probably accounts for the previously observed accumulation of FAEE in these organs...
  25. ncbi request reprint Effects of combined treatment with pravastatin and ursodeoxycholic acid on hepatic cholesterol metabolism
    C G Hillebrant
    Karoliniska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Eur J Clin Invest 32:528-34. 2002
    ....
  26. ncbi request reprint Acyl-coenzyme A:cholesterol O-acyltransferase is not identical to liver microsomal carboxylesterase
    M A Diczfalusy
    Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, Sweden
    Arterioscler Thromb Vasc Biol 16:606-10. 1996
    ..It is obvious that final identification of ACAT requires demonstration of an active homogenous protein...
  27. ncbi request reprint Activation of rat liver cytosolic phosphatidic acid phosphatase by nucleoside diphosphates
    L Berglund
    Department of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, Sweden
    Biochim Biophys Acta 1002:382-7. 1989
    ..The possibility is discussed that these effects might be of importance for the regulation of triacylglycerol biosynthesis...
  28. ncbi request reprint Phytosterolaemia in a Norwegian family: diagnosis and characterization of the first Scandinavian case
    D Lutjohann
    Department of Medical Laboratory Sciences and Technology, Karolinska Institute, Huddinge University Hospital, Sweden
    Scand J Clin Lab Invest 56:229-40. 1996
    ..Absorption of cholesterol averaged 63% in the patient and ranged from 35 to 45% in the relatives. Cholesterol synthesis appeared to be reduced in the patient and was 46-52% of that of her relatives...
  29. ncbi request reprint Genes involved in initial steps of bile acid synthesis
    I Bjorkhem
    Division of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, Huddinge, Sweden
    Curr Opin Lipidol 12:97-103. 2001
    ..During the past 2 years the genes coding for three of the six enzymes catalysing the first steps in bile acid biosynthesis have been cloned and characterized. These genes and their gene products will be described here...
  30. ncbi request reprint Patients with atherosclerosis may have increased circulating levels of 27-hydroxycholesterol and cholestenoic acid
    A Babiker
    Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Scand J Clin Lab Invest 65:365-75. 2005
    ..At the present state of knowledge, measurements of circulating levels of 27-oxygenated metabolites do not seem to add useful information about the atherosclerotic process...
  31. ncbi request reprint Cholesterol homeostasis in human brain: turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation
    I Bjorkhem
    Division of Clinical Chemistry, Karolinska Institute, Huddinge University Hospital, Sweden
    J Lipid Res 39:1594-600. 1998
    ..The possibility is discussed that circulating levels of 24S-hydroxycholesterol can be used as a marker for pathological and/or developmental changes in the brain...
  32. ncbi request reprint Cholestatic liver disease in adults may be due to an inherited defect in bile acid biosynthesis
    B Fischler
    Department of Pediatrics, Karolinska University Hospital, Huddinge, Sweden
    J Intern Med 262:254-62. 2007
    ..An increasing number of treatable inborn errors of bile acid synthesis have been described, primarily in infants with severe cholestatic liver disease...
  33. ncbi request reprint Platelet alpha- and beta- secretase activities are not significantly affected by dementia or mild cognitive impairment in Swedish patients
    P Gorham
    Department of Neurobiology, Karolinska Institutet and Karolinska University Hospital Huddinge, Stockholm, Sweden
    Curr Alzheimer Res 7:134-9. 2010
    ..The results are discussed in the context of the clinical usefulness of the secretase ratio as a biochemical adjunct to the diagnosis of AD...
  34. ncbi request reprint Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns
    M Gafvels
    Division of Clinical Chemistry, Karolinska Institute at Huddinge University Hospital, Huddinge, S 141 86, Sweden
    Genomics 56:184-96. 1999
    ..This is the first mammalian cytochrome P-450 gene reported to lack introns. The importance of this structural feature for evolution and gene regulation is discussed...
  35. ncbi request reprint Microsomal long-chain acyl-CoA thioesterase (carboxylesterase ES-4) is regulated by thyroxine
    M A Diczfalusy
    Department of Medical Laboratory Sciences and Technology, Division of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, S 141 86, Huddinge, Sweden
    Biochim Biophys Acta 1439:40-6. 1999
    ....
  36. ncbi request reprint Evidence that the major oxysterols in human circulation originate from distinct pools of cholesterol: a stable isotope study
    S Meaney
    Division of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, SE-141 86 Huddinge, Sweden
    J Lipid Res 42:70-8. 2001
    ..Lütjohann, K. von Bergmann, A. Wennmalm, U. Diczfalusy, and I. Björkhem. Evidence that the major oxysterols in human circulation originate from distinct pools of cholesterol: a stable isotope study. J. Lipid Res. 2001. 42: 70;-78...
  37. ncbi request reprint Molecular cloning and expression of rabbit sterol 12alpha-hydroxylase
    G Eggertsen
    Division of Clinical Chemistry, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden
    J Biol Chem 271:32269-75. 1996
    ..5 and 4.5 kilobases, respectively. Fasting of rats and mice led to a severalfold increase in both enzyme activity and mRNA levels. In contrast, starvation of rabbits had little or no stimulatory effect on enzyme activity and mRNA levels...
  38. ncbi request reprint Identification of unusual 7-oxygenated bile acid sulfates in a patient with Niemann-Pick disease, type C
    G Alvelius
    Clinical Research Center, Karolinska Institutet, Huddinge University Hospital, SE-14186 Stockholm, Sweden
    J Lipid Res 42:1571-7. 2001
    ..Based on previous data concerning the effects of 3beta-hydroxy-Delta(5) bile acids on bile acid transport, it is suggested that the formation of such bile acids is responsible for the cholestasis in this patient...