Elias S J Arner

Summary

Affiliation: Karolinska Institutet
Country: Sweden

Publications

  1. ncbi request reprint The thioredoxin system in cancer
    Elias S J Arner
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Semin Cancer Biol 16:420-6. 2006
  2. doi request reprint Redox pioneer: Professor Arne Holmgren
    Elias S J Arner
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Antioxid Redox Signal 15:845-51. 2011
  3. doi request reprint Focus on mammalian thioredoxin reductases--important selenoproteins with versatile functions
    Elias S J Arner
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochim Biophys Acta 1790:495-526. 2009
  4. doi request reprint Selenoproteins-What unique properties can arise with selenocysteine in place of cysteine?
    Elias S J Arner
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Exp Cell Res 316:1296-303. 2010
  5. ncbi request reprint Overexpression of enzymatically active human cytosolic and mitochondrial thioredoxin reductase in HEK-293 cells. Effect on cell growth and differentiation
    Ivan Nalvarte
    Department of Biosciences at Novum, Center for Biotechnology, Karolinska Institutet, SE 141 57 Huddinge, Sweden
    J Biol Chem 279:54510-7. 2004
  6. ncbi request reprint Regeneration of the antioxidant ubiquinol by lipoamide dehydrogenase, thioredoxin reductase and glutathione reductase
    Tomas Nordman
    Department of Laboratory Medicine, F 46, Karolinska Institutet, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    Biofactors 18:45-50. 2003
  7. ncbi request reprint Selenolthiol and dithiol C-terminal tetrapeptide motifs for one-step purification and labeling of recombinant proteins produced in E. coli
    Qing Cheng
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
    Chembiochem 7:1976-81. 2006
  8. ncbi request reprint Site-specifically 11C-labeled Sel-tagged annexin A5 and a size-matched control for dynamic in vivo PET imaging of protein distribution in tissues prior to and after induced cell death
    Qing Cheng
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Biochim Biophys Acta 1830:2562-73. 2013
  9. ncbi request reprint Exploiting the 21st amino acid-purifying and labeling proteins by selenolate targeting
    Linda Johansson
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Nat Methods 1:61-6. 2004
  10. doi request reprint Thiophosphate and selenite conversely modulate cell death induced by glutathione depletion or cisplatin: effects related to activity and Sec contents of thioredoxin reductase
    Xiaoxiao Peng
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem J 447:167-74. 2012

Collaborators

Detail Information

Publications83

  1. ncbi request reprint The thioredoxin system in cancer
    Elias S J Arner
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Semin Cancer Biol 16:420-6. 2006
    ..Recent data suggest that TrxR is essential for the carcinogenic process and invasive phenotype of cancer. Both Trx and TrxR have been regarded as interesting targets for chemotherapy...
  2. doi request reprint Redox pioneer: Professor Arne Holmgren
    Elias S J Arner
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Antioxid Redox Signal 15:845-51. 2011
    ..Dr. Holmgren helped lay the foundation for the redox biology field and opened new vistas in the process. He is truly a redox pioneer...
  3. doi request reprint Focus on mammalian thioredoxin reductases--important selenoproteins with versatile functions
    Elias S J Arner
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochim Biophys Acta 1790:495-526. 2009
    ..Clearly, a detailed understanding of mammalian thioredoxin reductases is necessary for a full comprehension of the thioredoxin system and of selenium dependent processes in mammals...
  4. doi request reprint Selenoproteins-What unique properties can arise with selenocysteine in place of cysteine?
    Elias S J Arner
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Exp Cell Res 316:1296-303. 2010
    ....
  5. ncbi request reprint Overexpression of enzymatically active human cytosolic and mitochondrial thioredoxin reductase in HEK-293 cells. Effect on cell growth and differentiation
    Ivan Nalvarte
    Department of Biosciences at Novum, Center for Biotechnology, Karolinska Institutet, SE 141 57 Huddinge, Sweden
    J Biol Chem 279:54510-7. 2004
    ..Most surprisingly, the TrxR1- or TrxR2-overexpressing cells also induced novel expression of the epithelial markers CK18, CK-Cam5.2, and BerEP4, suggestive of a stimulation of cellular differentiation...
  6. ncbi request reprint Regeneration of the antioxidant ubiquinol by lipoamide dehydrogenase, thioredoxin reductase and glutathione reductase
    Tomas Nordman
    Department of Laboratory Medicine, F 46, Karolinska Institutet, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    Biofactors 18:45-50. 2003
    ..The reduction of ubiquinone by the three enzymes offers a multifunctional system for extramitochondrial regeneration of an important antioxidant...
  7. ncbi request reprint Selenolthiol and dithiol C-terminal tetrapeptide motifs for one-step purification and labeling of recombinant proteins produced in E. coli
    Qing Cheng
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
    Chembiochem 7:1976-81. 2006
    ..It should therefore be possible to utilize these multifunctional tetrapeptide motifs to develop a wide range of novel biotechnological applications based on Sec targeting with electrophilic compounds...
  8. ncbi request reprint Site-specifically 11C-labeled Sel-tagged annexin A5 and a size-matched control for dynamic in vivo PET imaging of protein distribution in tissues prior to and after induced cell death
    Qing Cheng
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Biochim Biophys Acta 1830:2562-73. 2013
    ..We describe here a new method for labeling AnxA5 and a size-matched control protein with short-lived carbon-11, for probing the specificity of in vivo cell death monitoring using positron emission tomography (PET) imaging...
  9. ncbi request reprint Exploiting the 21st amino acid-purifying and labeling proteins by selenolate targeting
    Linda Johansson
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Nat Methods 1:61-6. 2004
    ..The results presented here thus show how a wide range of biotechnological applications can be developed starting from the insertion of selenocysteine into proteins...
  10. doi request reprint Thiophosphate and selenite conversely modulate cell death induced by glutathione depletion or cisplatin: effects related to activity and Sec contents of thioredoxin reductase
    Xiaoxiao Peng
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem J 447:167-74. 2012
    ..The results suggest molecular mechanisms by which the selenium status of cells can affect their glutathione dependence while modulating the cytotoxicity of drugs that target TrxR1...
  11. ncbi request reprint Inhibition of thioredoxin reductase but not of glutathione reductase by the major classes of alkylating and platinum-containing anticancer compounds
    Anne Barbara Witte
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 39:696-703. 2005
    ..The TrxR inhibition should thereby be considered as a factor that may contribute to the cytotoxicity seen upon clinical use of these drugs...
  12. ncbi request reprint Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C
    Qing Cheng
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Nat Protoc 1:604-13. 2006
    ..We also describe how the Sel-tag can be used as a basis for efficient one-step protein purification, rapid Sec-targeting protein labeling with electrophilic compounds, or radiolabeling with the positron emitter 11C...
  13. pmc Biochemical discrimination between selenium and sulfur 1: a single residue provides selenium specificity to human selenocysteine lyase
    Ruairi Collins
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    PLoS ONE 7:e30581. 2012
    ..Our findings thus provide molecular insights into a key level of control in human selenium and selenoprotein turnover and metabolism...
  14. pmc Cell death by SecTRAPs: thioredoxin reductase as a prooxidant killer of cells
    Karin Anestål
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 3:e1846. 2008
    ..SecTRAPs are devoid of thioredoxin reductase activity but can induce rapid cell death in cultured cancer cell lines by a gain of function...
  15. doi request reprint Highly active dimeric and low-activity tetrameric forms of selenium-containing rat thioredoxin reductase 1
    Olle Rengby
    Division of Biochemistry, Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 46:893-904. 2009
    ..The tetrameric forms of the protein could represent hitherto unknown regulatory states of the enzyme...
  16. pmc The rare TXNRD1_v3 ("v3") splice variant of human thioredoxin reductase 1 protein is targeted to membrane rafts by N-acylation and induces filopodia independently of its redox active site integrity
    Marcus Cebula
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 288:10002-11. 2013
    ..These results identify v3 as an intricately regulated protein that expands TXNRD1-derived protein functions to the membrane raft compartment...
  17. doi request reprint High levels of thioredoxin reductase 1 modulate drug-specific cytotoxic efficacy
    Sofi E Eriksson
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 47:1661-71. 2009
    ..The results suggest that high overexpression of TrxR has an impact not necessarily linked to Trx function that nonetheless modulates drug-specific cytotoxic responses...
  18. pmc Inhibition of thioredoxin reductase 1 by porphyrins and other small molecules identified by a high-throughput screening assay
    Stefanie Prast-Nielsen
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 50:1114-23. 2011
    ..Finally, additional inhibitors of TrxR1 may be discovered and further characterized based upon the new high-throughput TrxR1 assay presented here...
  19. pmc Wobble decoding by the Escherichia coli selenocysteine insertion machinery
    Jianqiang Xu
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm SE 171 77, Sweden and Division of Physiological Chemistry I, Department of Medical Biochemistry and Biophysics, Proteomics Karolinska PK KI, Karolinska Institutet, Stockholm SE 171 77, Sweden
    Nucleic Acids Res 41:9800-11. 2013
    ..The results also reveal that the E. coli selenoprotein synthesis machinery has the inherent capability to promote wobble decoding. ..
  20. ncbi request reprint Recombinant expression of mammalian selenocysteine-containing thioredoxin reductase and other selenoproteins in Escherichia coli
    Elias S J Arner
    Department of Medical Biochemistry and Biophysics, Medical Nobel Institute for Biochemistry, Karolinska Institute, SE 17177 Stockholm, Sweden
    Methods Enzymol 347:226-35. 2002
  21. pmc Biochemical discrimination between selenium and sulfur 2: mechanistic investigation of the selenium specificity of human selenocysteine lyase
    Ann Louise Johansson
    Arrhenius Laboratories for Natural Sciences C4, Stockholm Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    PLoS ONE 7:e30528. 2012
    ..Based on these results and previously available data we propose a reaction mechanism whereby the Sec over Cys specificity is achieved using a combination of chemical and physico-mechanical control mechanisms...
  22. doi request reprint Pyrroloquinoline quinone modulates the kinetic parameters of the mammalian selenoprotein thioredoxin reductase 1 and is an inhibitor of glutathione reductase
    Jianqiang Xu
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem Pharmacol 83:815-20. 2012
    ..These findings may help explain several of the effects of PQQ seen in mammals...
  23. ncbi request reprint Induction of cell membrane protrusions by the N-terminal glutaredoxin domain of a rare splice variant of human thioredoxin reductase 1
    Pascal Dammeyer
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    J Biol Chem 283:2814-21. 2008
    ..The results presented thus reveal that TXNRD1_v3 has a unique and distinct expression pattern in human cells and suggest that the protein can guide actin polymerization in relation to cell membrane restructuring...
  24. doi request reprint Crystal structure and catalysis of the selenoprotein thioredoxin reductase 1
    Qing Cheng
    Division of Biochemistry, Medical Nobel Institute for Biochemistry, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 284:3998-4008. 2009
    ....
  25. doi request reprint Noble metal targeting of thioredoxin reductase--covalent complexes with thioredoxin and thioredoxin-related protein of 14 kDa triggered by cisplatin
    Stefanie Prast-Nielsen
    Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Free Radic Biol Med 49:1765-78. 2010
    ..These results reveal that Au and Pd are strong inhibitors of TrxR, but Pt and cisplatin trigger highly specific cellular effects on the Trx system, including covalent cross-linking of TrxR1 with Trx1 and TRP14...
  26. ncbi request reprint Studies of an active site mutant of the selenoprotein thioredoxin reductase: the Ser-Cys-Cys-Ser motif of the insect orthologue is not sufficient to replace the Cys-Sec dyad in the mammalian enzyme
    Linda Johansson
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 41:649-56. 2006
    ..These findings suggest that features in addition to the flanking Ser residues are important for facilitating the high activity of the insect enzyme and that the corresponding features are absent in mammalian TrxR...
  27. ncbi request reprint A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation
    Linda Johansson
    Department of Medicine, Clinical Immunology and Allergy Unit, Karolinska Institute and University Hospital, Stockholm, Sweden
    FEBS J 272:3449-60. 2005
    ..Thus, an allergic response to the inhaled allergen may lead to prolonged retention of Der p 2 in the lung...
  28. ncbi request reprint Selenocysteine in proteins-properties and biotechnological use
    Linda Johansson
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Biochim Biophys Acta 1726:1-13. 2005
    ....
  29. ncbi request reprint Regulation of the mammalian selenoprotein thioredoxin reductase 1 in relation to cellular phenotype, growth, and signaling events
    Anna Klara Rundlöf
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Antioxid Redox Signal 6:41-52. 2004
    ..Here, we summarize the current knowledge of these different levels of TrxR1 regulation in diverse cell types and in response to growth and signaling events...
  30. pmc Titration and conditional knockdown of the prfB gene in Escherichia coli: effects on growth and overproduction of the recombinant mammalian selenoprotein thioredoxin reductase
    Olle Rengby
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Appl Environ Microbiol 73:432-41. 2007
    ....
  31. ncbi request reprint p53-dependent inhibition of TrxR1 contributes to the tumor-specific induction of apoptosis by RITA
    Elisabeth Hedström
    Department for Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Cell Cycle 8:3576-83. 2009
    ....
  32. doi request reprint Effects of selenite and chelating agents on mammalian thioredoxin reductase inhibited by mercury: implications for treatment of mercury poisoning
    Cristina M L Carvalho
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    FASEB J 25:370-81. 2011
    ..These results stress the role of TrxR as a target of mercurials and provide the mechanism of selenite as a detoxification agent for mercury poisoning...
  33. pmc Selective activation of oxidized PTP1B by the thioredoxin system modulates PDGF-β receptor tyrosine kinase signaling
    Markus Dagnell
    Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institutet, SE 171 76 Stockholm, Sweden
    Proc Natl Acad Sci U S A 110:13398-403. 2013
    ..The studies demonstrate a previously unrecognized pathway for selective redox-regulated control of receptor tyrosine kinase signaling. ..
  34. doi request reprint Human Protein Atlas of redox systems - what can be learnt?
    Pascal Dammeyer
    Department of Medical Biochemistry and Biophyscis, Karolinska Institutet, Stockholm, Sweden
    Biochim Biophys Acta 1810:111-38. 2011
    ..Here, we wished to probe what could be learnt from this atlas using a manual in-depth analysis of the results regarding the expression of key proteins in the human glutathione and thioredoxin systems...
  35. pmc Cisplatin and oxaliplatin toxicity: importance of cochlear kinetics as a determinant for ototoxicity
    Victoria Hellberg
    Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
    J Natl Cancer Inst 101:37-47. 2009
    ..Cisplatin is a cornerstone anticancer drug with pronounced ototoxicity, whereas oxaliplatin, a platinum derivative with a different clinical profile, is rarely ototoxic. This difference has not been explained...
  36. ncbi request reprint Evidence for intriguingly complex transcription of human thioredoxin reductase 1
    Anna Klara Rundlöf
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Free Radic Biol Med 36:641-56. 2004
    ..Cell-specific transcription of the TXNRD1 gene encoding different isoforms of TrxR1 must be taken into account to fully understand the functions of the human thioredoxin system...
  37. doi request reprint Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells
    Lena Ekström
    Division of Clinical Pharmacology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, SE 141 86 Stockholm, Sweden
    Biochem Biophys Res Commun 430:90-4. 2013
    ..Thus, our results reveal a simvastatin-specific reduction of cellular TrxR1 levels that at least in part involves direct inhibitory effects on the basal activity of the core promoter guiding TrxR1 expression...
  38. doi request reprint HER2-positive tumors imaged within 1 hour using a site-specifically 11C-labeled Sel-tagged affibody molecule
    Helena Wållberg
    Division of Molecular Biotechnology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden
    J Nucl Med 53:1446-53. 2012
    ..In vivo imaging methods are available but not yet in clinical practice; new methodologies improving speed, sensitivity, and specificity are required...
  39. doi request reprint Red wine triggers cell death and thioredoxin reductase inhibition: effects beyond resveratrol and SIRT1
    Karolina Wallenborg
    CoE in Developmental Biology for Regenerative Medicine CEDB DBRM, Department of Neuroscience, Karolinska Institutet, SE17177, Stockholm, Sweden
    Exp Cell Res 315:1360-71. 2009
    ..Our results unveil important roles for SIRT1 and TrxR in resveratrol and red wine-mediated effects on progenitor and cancer cells, and demonstrate that cellular responses to red wine may be more complex than generally appreciated...
  40. ncbi request reprint Rapid induction of cell death by selenium-compromised thioredoxin reductase 1 but not by the fully active enzyme containing selenocysteine
    Karin Anestål
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, 171 77 Stockholm, Sweden
    J Biol Chem 278:15966-72. 2003
    ..The direct cellular effects of selenium-compromised forms of TrxR1 may be important for the pathophysiology of selenium deficiency as well as for the efficacy of antiproliferative drugs targeting the selenocysteine moiety of this enzyme...
  41. pmc Assessment of production conditions for efficient use of Escherichia coli in high-yield heterologous recombinant selenoprotein synthesis
    Olle Rengby
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, 171 77 Stockholm, Sweden
    Appl Environ Microbiol 70:5159-67. 2004
    ..This study also demonstrates that E. coli can indeed be utilized as cell factories for highly efficient production of heterologous selenoproteins such as rat thioredoxin reductase...
  42. ncbi request reprint The mammalian cytosolic selenoenzyme thioredoxin reductase reduces ubiquinone. A novel mechanism for defense against oxidative stress
    Ling Xia
    Department of Microbiology, Pathology, and Immunology, Division of Pathology, F46, Karolinska Institutet, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    J Biol Chem 278:2141-6. 2003
    ..These data demonstrate that the selenoenzyme thioredoxin reductase is an important selenium-dependent ubiquinone reductase and can explain how selenium and ubiquinone, by a combined action, may protect the cell from oxidative damage...
  43. pmc The redox state of SECIS binding protein 2 controls its localization and selenocysteine incorporation function
    Laura V Papp
    Queensland Institute of Medical Research, 300 Herston Road, Herston, Queensland 4029, Australia
    Mol Cell Biol 26:4895-910. 2006
    ....
  44. ncbi request reprint Motexafin gadolinium, a tumor-selective drug targeting thioredoxin reductase and ribonucleotide reductase
    Seyed Isaac Hashemy
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    J Biol Chem 281:10691-7. 2006
    ..This may explain the effects of the drug on cancer cells, which often overproduce TrxR and have induced RNR for replication and repair...
  45. ncbi request reprint Human mitochondrial glutaredoxin reduces S-glutathionylated proteins with high affinity accepting electrons from either glutathione or thioredoxin reductase
    Catrine Johansson
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 171 77 Stockholm, Sweden
    J Biol Chem 279:7537-43. 2004
    ..These results suggest an important role for Grx2 in protection and recovery from oxidative stress...
  46. ncbi request reprint PSK and Trx80 inhibit B-cell growth in EBV-infected cord blood mononuclear cells through T cells activated by the monocyte products IL-15 and IL-12
    Anquan Liu
    Microbiology and Tumor Biology Center, Karolinska Institute, Box 280, 171 77 Stockholm, Sweden
    Blood 105:1606-13. 2005
    ..Phosphatidylinositol 3-(PI 3)-kinase and reactive oxygen species (ROSs) were involved in the PSK-induced activation of monocytes. Restimulation of the cultures with EBV-transformed B cells generated specific cytotoxic activity...
  47. doi request reprint Biochemistry. SNO removal
    Arne Holmgren
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Science 320:1019-20. 2008
  48. ncbi request reprint Selenite in cancer therapy: a commentary on "Selenite induces apoptosis in sarcomatoid malignant mesothelioma cells through oxidative stress"
    Arne Holmgren
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Free Radic Biol Med 41:862-5. 2006
  49. ncbi request reprint Thioredoxin reductase is irreversibly modified by curcumin: a novel molecular mechanism for its anticancer activity
    Jianguo Fang
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 17177 Stockholm, Sweden
    J Biol Chem 280:25284-90. 2005
    ..Modification of TrxR by curcumin provides a possible mechanistic explanation for its cancer preventive activity, shifting the enzyme from an antioxidant to a prooxidant...
  50. ncbi request reprint Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function
    Aristi Potamitou
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 277:18561-7. 2002
    ..The levels of Grx2 and Grx3 behaved exactly the opposite, suggesting that the transcription factor OxyR does not regulate their expression...
  51. ncbi request reprint Truncated thioredoxin: physiological functions and mechanism
    Klas Pekkari
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Antioxid Redox Signal 6:53-61. 2004
    ..Trx80 and IL-2 together were strongly synergistic to induce secretion of interferon-gamma in PBMC. Trx80 is a potent cytokine for monocytes directing the immune system to a Th1 response via IL-12 production...
  52. ncbi request reprint Expression of the thioredoxin system in interstitial lung disease
    Leena Tiitto
    Department of Internal Medicine, University of Oulu and Oulu University Hospital, Oulu, Finland
    J Pathol 201:363-70. 2003
    ....
  53. ncbi request reprint Real-time kinetics of the interaction between the two subunits, Escherichia coli thioredoxin and gene 5 protein of phage T7 DNA polymerase
    Netai C Singha
    Medical Nobel Institute for Biochemistry, Karolinska Institute, Stockholm S 171 77, Sweden
    J Biol Chem 278:21421-8. 2003
    ..2 kcal.m-1) and entropy (TDeltaS =-8.4 kcal.m-1) upon formation of the complex suggested that the interaction is driven by an increase in enthalpy and opposed by a decrease in entropy...
  54. pmc Glutathionylation of human thioredoxin: a possible crosstalk between the glutathione and thioredoxin systems
    Simona Casagrande
    Mario Negri Institute for Pharmacological Research, Department of Biochemistry, 20157 Milan, Italy
    Proc Natl Acad Sci U S A 99:9745-9. 2002
    ..These data suggest that the intracellular glutathione/glutathione disulfide ratio, an indicator of the redox state of the cell, can regulate Trx functions reversibly through thiol-disulfide exchange reactions...
  55. ncbi request reprint A novel antioxidant mechanism of ebselen involving ebselen diselenide, a substrate of mammalian thioredoxin and thioredoxin reductase
    Rong Zhao
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 277:39456-62. 2002
    ..We propose novel mechanisms for ebselen antioxidant action involving ebselen selenol and diselenide formation, with the thioredoxin system rather than glutathione as the predominant effector and target...
  56. pmc Chaperone properties of Escherichia coli thioredoxin and thioredoxin reductase
    Renee Kern
    Stress Molecules, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, 75005 Paris, France
    Biochem J 371:965-72. 2003
    ..These results suggest that the thioredoxin system, in addition to its protein disulphide isomerase activity possesses chaperone-like properties, and that its thioredoxin reductase component plays a major role in this function...
  57. ncbi request reprint Truncated thioredoxin (Trx80) exerts unique mitogenic cytokine effects via a mechanism independent of thiol oxido-reductase activity
    Klas Pekkari
    Medical Nobel Institute for Biochemistry, Department of Biophysics and Biochemistry, Karolinska Institutet, 171 77 Stockholm, Sweden
    FEBS Lett 539:143-8. 2003
    ..01, n=9 and 10). The previously described Trx80 dimer is caused by non-covalent interactions, and not by any intermolecular disulphide bonds...
  58. ncbi request reprint Identification of S-glutathionylated cellular proteins during oxidative stress and constitutive metabolism by affinity purification and proteomic analysis
    Christina Lind
    Division of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska Institute, Stockholm, Sweden
    Arch Biochem Biophys 406:229-40. 2002
    ....
  59. ncbi request reprint Human spermatid-specific thioredoxin-1 (Sptrx-1) is a two-domain protein with oxidizing activity
    Alberto Jimenez
    Center for Biotechnology, Department of Biosciences at Novum, Karolinska Institutet, S 14157 Huddinge, Sweden
    FEBS Lett 530:79-84. 2002
    ..This oxidizing enzymatic activity suggests that Sptrx-1 might govern the stabilization (by disulfide cross-linking) of the different structures in the developing tail of spermatids and spermatozoa...
  60. ncbi request reprint Visualization of the compartmentalization of glutathione and protein-glutathione mixed disulfides in cultured cells
    Therese Söderdahl
    Division of Biochemical Toxicology, Karolinska Institutet, Stockholm Sweden
    FASEB J 17:124-6. 2003
    ..Similar FACS analyses performed in isolated mitochondria presented a considerable variation in GSH content within mitochondria of uniform granularity from the same preparation...
  61. ncbi request reprint Thiol-based mechanisms of the thioredoxin and glutaredoxin systems: implications for diseases in the cardiovascular system
    Carsten Berndt
    The Medical Nobel Inst for Biochemistry, Dept of Medical Biochemistry and Biophysics, Karolinska Inst, SE 17177 Stockholm, Sweden
    Am J Physiol Heart Circ Physiol 292:H1227-36. 2007
    ....
  62. ncbi request reprint Glutaredoxins: glutathione-dependent redox enzymes with functions far beyond a simple thioredoxin backup system
    Aristi Potamitou Fernandes
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Antioxid Redox Signal 6:63-74. 2004
    ..Glutaredoxins also catalyze formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential...
  63. ncbi request reprint Thioredoxin is associated with proliferation, p53 expression and negative estrogen and progesterone receptor status in breast carcinoma
    Nina Turunen
    Department of Pathology, Oulu University Hospital, Oulu, Finland
    APMIS 112:123-32. 2004
    ....
  64. ncbi request reprint Cellular and plasma levels of human glutaredoxin 1 and 2 detected by sensitive ELISA systems
    Mathias Lundberg
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 319:801-9. 2004
    ..This effect was shown to occur at the transcriptional level. The secretion of Grx1 and its presence in plasma suggests extracellular functions as found for mammalian thioredoxin 1...
  65. ncbi request reprint Overexpression of glutaredoxin 2 attenuates apoptosis by preventing cytochrome c release
    Mari Enoksson
    Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 327:774-9. 2005
    ..We propose that Grx2 facilitates the maintenance of cellular redox homeostasis upon treatment with apoptotic agents, thereby preventing cardiolipin oxidation and cytochrome c release...
  66. ncbi request reprint Inhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity
    Jun Lu
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Sweden
    Cancer Res 66:4410-8. 2006
    ..Thus, the anticancer activity of quercetin and myricetin may be due to inhibition of TrxR, consequently inducing cell death...
  67. ncbi request reprint Inhibition of thioredoxin and thioredoxin reductase by 4-hydroxy-2-nonenal in vitro and in vivo
    Jianguo Fang
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 17177 Stockholm, Sweden
    J Am Chem Soc 128:1879-85. 2006
    ....
  68. ncbi request reprint Requirements for the different cysteines in the chemotactic and desensitizing activity of human thioredoxin
    Cinzia Bizzarri
    Dompe Research Center and Consorzio Biolaq, L Aquila, Italy
    Antioxid Redox Signal 7:1189-94. 2005
    ..These data indicate that different redox-active cysteines are important for Trx chemotactic action, whereas its desensitizing action does not have these requirements, suggesting a redox-independent mechanism...
  69. pmc Short interfering RNA-mediated silencing of glutaredoxin 2 increases the sensitivity of HeLa cells toward doxorubicin and phenylarsine oxide
    Christopher Horst Lillig
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 17177 Stockholm, Sweden
    Proc Natl Acad Sci U S A 101:13227-32. 2004
    ..These results indicate a crucial role of Grx2 in the regulation of the mitochondrial redox status and regulation of cell death at the mitochondrial checkpoint...
  70. ncbi request reprint Truncated thioredoxin (Trx80) induces differentiation of human CD14+ monocytes into a novel cell type (TAMs) via activation of the MAP kinases p38, ERK, and JNK
    Klas Pekkari
    Medical Nobel Institute for Biochemistry, Department of Biochemistry and Biophysics, Karolinska Institutet, SE 171 77, Stockholm, Sweden
    Blood 105:1598-605. 2005
    ..We propose that Trx80 is an early signal in response to danger, and that TAMs may play a major role in triggering innate immune responses...
  71. ncbi request reprint A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductase
    Aristi Potamitou Fernandes
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 280:24544-52. 2005
    ..Grx4 was highly elevated upon iron depletion, suggesting an iron-related function for the protein...
  72. ncbi request reprint The thioredoxin system in cancer-introduction to a thematic volume of Seminars in Cancer Biology
    Elias S J Arner
    Semin Cancer Biol 16:419. 2006
  73. ncbi request reprint Thioredoxin and related molecules--from biology to health and disease
    Christopher Horst Lillig
    The Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Antioxid Redox Signal 9:25-47. 2007
    ..Thioredoxin and binding proteins (ASK1 and TBP2) appear to control apoptosis or metabolic states such as carbohydrate and lipid metabolism related to diseases such as diabetes and atherosclerosis...
  74. ncbi request reprint Ebselen is a dehydroascorbate reductase mimic, facilitating the recycling of ascorbate via mammalian thioredoxin systems
    Rong Zhao
    Antioxid Redox Signal 6:99-104. 2004
    ..The high ascorbic acid recycling efficiency of ebselen at pH 6.4 may play a major role in oxidatively stressed cells, where cytosol acidosis may trigger various responses, including apoptosis...
  75. ncbi request reprint Expression pattern of human glutaredoxin 2 isoforms: identification and characterization of two testis/cancer cell-specific isoforms
    Maria Elisabet Lönn
    The Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Antioxid Redox Signal 10:547-57. 2008
    ..Expression of GFP fusion proteins suggested a cytosolic and nuclear localization of both Grx2b and Grx2c. Our findings provide the first evidence for functions of Grx2 outside mitochondria...
  76. pmc Targeting thioredoxin reductase is a basis for cancer therapy by arsenic trioxide
    Jun Lu
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 17177 Stockholm, Sweden
    Proc Natl Acad Sci U S A 104:12288-93. 2007
    ..Blocking cancer cell DNA replication and repair and induction of oxidative stress by the inhibition of both Trx and GSH systems are suggested as cancer chemotherapeutic strategies...
  77. ncbi request reprint High-resolution structures of oxidized and reduced thioredoxin reductase from Helicobacter pylori
    Tomas N Gustafsson
    The Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Acta Crystallogr D Biol Crystallogr 63:833-43. 2007
    ..coli shows that the overall surface charge distribution in these proteins is conserved and that residue substitutions that change the shape of the binding surface may account for the species-specific recognition of thioredoxin by TrxR...
  78. ncbi request reprint Molecular and structural basis for redox regulation of beta-actin
    Ingrid Lassing
    Department of Microbiology, Tumor Biology, and Cell Biology, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Mol Biol 370:331-48. 2007
    ..No other oxidative modification was obvious in the structure, highlighting the specificity of the oxidation by H(2)O(2). Possible consequences of the observed effects in a cellular context and their potential relevance are discussed...
  79. ncbi request reprint A conserved cis-proline precludes metal binding by the active site thiolates in members of the thioredoxin family of proteins
    Dan Su
    Department of Biochemistry, Univeristy of Nebraska Lincoln, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 46:6903-10. 2007
    ..Taken together, our data suggest that an important function of Pro75 in human thioredoxin, and likely other members of this superfamily, is to prevent metal binding by the reactive thiolate-based active site...
  80. ncbi request reprint Molecular mapping of functionalities in the solution structure of reduced Grx4, a monothiol glutaredoxin from Escherichia coli
    Malin Fladvad
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 280:24553-61. 2005
    ..Structural and functional implications are discussed with particular emphasis on identifying common monothiol glutaredoxin properties in substrate specificity and ligand binding events, linking the thioredoxin and glutaredoxin systems...
  81. ncbi request reprint Oxidation and S-nitrosylation of cysteines in human cytosolic and mitochondrial glutaredoxins: effects on structure and activity
    Seyed Isaac Hashemy
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    J Biol Chem 282:14428-36. 2007
    ..The striking differences between Grx1 and Grx2 reflect their diverse regulatory functions in vivo and also adaptation to different subcellular localization...
  82. ncbi request reprint How does iron-sulfur cluster coordination regulate the activity of human glutaredoxin 2?
    Carsten Berndt
    The Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Antioxid Redox Signal 9:151-7. 2007
    ..When reduced glutathione becomes the limiting factor for cluster coordination, the holo-Grx2 complex dissociates, yielding enzymatically active Grx2...
  83. ncbi request reprint Catalysis of thiol/disulfide exchange. Glutaredoxin 1 and protein-disulfide isomerase use different mechanisms to enhance oxidase and reductase activities
    Ruoyu Xiao
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 280:21099-106. 2005
    ....