S Janciauskiene

....

..In conclusion, the ATZ11 monoclonal antibody detects Z alpha(1)-antitrypsin in hepatic inclusions by detecting a neoepitope that is specific to the polymeric conformer and that is localized close to residue 342...

....

..Therefore, a "local relationship" between mast cells and airway epithelial cells might be an important step in the inflammatory response...

..The only known genetic factor related to COPD is inherited PiZZ deficiency of alpha1-antitrypsin (AAT), an inhibitor of serine proteases...

..There is increasing evidence that AAT is able to exert effects other than protease inhibition. We investigated whether Prolastin, a preparation of purified pooled human AAT used for augmentation therapy, exhibits anti-bacterial effects...

..01, 0.02, 0.05, 0.1, 0.5 and 1 mg/ml for 18 h, and analysed to determine the release of cytokines and to detect the activity of NF-kappaB...

..The antibody can be a powerful tool for the study of the molecular profile of AAT, not only in Z-deficiency cases but also in other (patho)physiological conditions...

..05). We show here for the first time that SLPI is present in AH and may play a role as well as serve as a marker in pathological states...

....

..These results suggest that cleavage of AAT may not only abolish its proteinase inhibitor activity, but can also generate a powerful pro-inflammatory activator for human neutrophils...

..This also supports important roles for CD36 and LDL receptors in atherogenesis and suggests that blockade of CD36 receptor can be protective in pro-inflammatory activation of human monocytes...

..Elucidation of the biological activities of non-inhibitory forms of serpins may provide useful insights into the pathogenesis of diseases and suggest new therapeutic strategies...

..Thus, our data demonstrate for the first time that AAT exhibits anti-inflammatory activity in vitro that is unrelated to inhibition of serine proteases...

..We conclude that interaction of AAT-(358-394)-peptide fibrils with cell surface receptor(s) disturbs intracellular cholesterol homeostasis and induces cell death...

....

..This may be important for an understanding of the events conducive to atherogenesis...

..4- to 5.5-fold. These data extend the findings of potential anti-inflammatory actions of statins and also suggest the possibility for pravastatin use in a broader spectrum of inflammatory situations...

..These data suggest a possible new role for Abeta in Alzheimer's disease through its interaction with the inflammatory reactant, ACT...

....

..In contrast to native and enzymatically ox LDL, copper ox LDL does not induce intracellular lipid accumulation. Differently oxidized LDL molecules may exert distinct effects in lesion development in atherosclerosis...

....

..The presence of Abeta and inflammation-associated proteins in aqueous from cataract cases without detectable PEX raises the possibility that these proteins may reflect early amyloid-related changes in the eye...

..Experimental and clinical investigations have revealed that statins can downregulate both acute and chronic inflammatory processes. Whether statins express anti-inflammatory activities in the treatment of Crohn's disease is unknown...

..No size difference was noted on SDS-PAGE. These results suggest the occurrence of noncovalent complex formation between cholesterol and alpha 1-PI in vitro...

....

..To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-gamma) in primary human monocytes in culture...

..We suggest that C-36 mediates its effects though the activation of LPS signaling pathways...

..Serine proteinase inhibitor, AAT, dependent on its molecular form, is also found to exert diverse effects on the properties of tumour cells confirming the complexity of cell-protein interaction...

..Their role in gallstone pathogenesis is unclear, and it was the aim of this study to determine their effect on cholesterol crystal growth rate in model bile...

..These results indicate that AAT, dependent on its molecular form, can both suppress and induce breast tumour cell biological activity in vitro...

..These observations highlight the importance of the finding sets of biomolecules, which could offer new strategies for the diagnosis of COPD and may have value for monitoring progression of COPD...

..Because statins can reduce chemokine expression in patients with coronary diseases, we wanted to test whether this can be achieved in patients with Crohn's disease...

..Aldehyde-modified peptide sequences in apolipoprotein B-100 (apoB-100) are major targets for these immune responses...

..Since matriptase is involved in a number of physiologic processes, including activation of epithelial sodium channels, our findings offer considerable new insights into new regulatory function of AAT in vivo...

..Treatment with atorvastatin may be a potential strategy to reduce oxLDL and inhibit monocyte migration to inflamed tissue, thus attenuating the inflammatory response...

..These data corroborate previous findings of the pleiotropic role of statins and also suggest the involvement of transcription factors such as PPAR gamma and NF kappa B in the modulation of the inflammatory processes by statins...

..These data indicate that soluble forms of Abeta(1-42), which are a potent stimulus of pro-inflammatory activation of glioma cells in vitro, could be a good target for pravastatin...

..e. ulcerative colitis and Crohn's disease. These mechanisms might provide attractive targets for novel therapies...

..The data suggest alpha 1PI-cholesterol binding to be affected by both direct and indirect modifications of the alpha 1PI-reactive center, that is situated on a mobile peptide loop...

..It is concluded that cystatin C through this effect can act as an immunomodulatory molecule...

..These findings suggest that pause time (in contrast to articulation time) may serve as a sensitive measure in the assessment of information processing speed deficits in dementia, by virtue of its close association with brain pathology...

..CONCLUSIONS: This is the first demonstration that alpha1-AT bound to the vascular endothelium of lungs is in a polymeric form, which also suggests a possible previously unknown role for polymeric alpha1-AT in vivo...

..We have investigated whether plasma AAT levels are altered in Type 2 diabetes...

..On the basis of these findings it is suggested that by releasing AAT, eosinophils may, in a microenvironment, play a role in counteracting the tissue damage caused by serine proteinases released by neutrophils in inflammatory conditions...

....

....

..A number of studies have revealed variations in the plasma levels of serine proteases and their inhibitors among tumor types...

..Thus, our data demonstrate that AAT inhibits MID-induced B cell activation in vitro that is unrelated to its protease inhibitory activity and is not dependent on MID binding to the cell surface...

..In conclusion, C4BP binds to dead brain cells and Abeta peptide in vitro, is present in CSF and possibly protects against excessive complement activation in AD brains...

..These findings may also explain the progression of emphysema in some individuals despite alpha(1)-antitrypsin replacement therapy...

..These results suggest that peptides derived from specific serum proteins may alter hepatic gene expression in a highly specific manner...

..Cleaved form of AAT had no significant effects on parameters measured. Data obtained from this study suggest that specific forms of AAT have multiple effects on tumor cell viability and play diverse roles in tumorogenesis...