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Genomes and Genes | Stefan HohmannSummaryCountry: Sweden Publications
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Publications
Thiamin metabolism and thiamin diphosphate-dependent enzymes in the yeast Saccharomyces cerevisiae: genetic regulationS Hohmann
Department of General and Marine Microbiology, Goteborg University, Medicinaregatan 9C, S 41390 Göteborg, Sweden
Biochim Biophys Acta 1385:201-19. 1998..Apparently, the production of ThDP and of the enzymes using this cofactor is coordinately regulated. Future research will focus on the elucidation of the molecular mechanisms of this novel type of regulation...- Microbial MIP channelsI Hohmann
Dept of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, S 40530 Göteborg, Sweden
Trends Microbiol 8:33-8. 2000..As a result of their involvement in osmoregulation and metabolism, MIP channels are believed to affect a wide range of biological processes... 
Osmotic stress signaling and osmoadaptation in yeastsStefan Hohmann
Department of Cell and Molecular Biology Microbiology, Goteborg University, S 405 30 Goteborg, Sweden
Microbiol Mol Biol Rev 66:300-72. 2002....- The Yeast Systems Biology Network: mating communitiesStefan Hohmann
Department of Cell and Molecular Biology, Goteborg University, Box 462, S 40530 Göteborg, Sweden
Curr Opin Biotechnol 16:356-60. 2005..The yeast Saccharomyces cerevisiae serves as an excellent model system. To coordinate efforts and to bring the yeast research and systems biology communities together, the Yeast Systems Biology Network has recently been started... 
The pathway by which the yeast protein kinase Snf1p controls acquisition of sodium tolerance is different from that mediating glucose regulationTian Ye
Department of Cell and Molecular Biology Microbiology, University of Gothenburg, Box 462, S 40530 Göteborg, Sweden
Microbiology 154:2814-26. 2008..Snf1p appears to be part of a wider functional network than previously anticipated and the full complexity of this network remains to be elucidated...- Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shockMarcus Krantz
Department of Cell and Molecular Biology/Microbiology, , Box 462, , Sweden
Eukaryot Cell 3:1381-90. 2004..These observations demonstrate the crucial role of glycerol accumulation and turgor recovery in determining the period of osmotic shock-induced signaling and the profile of cellular adaptation to osmotic shock... - Comparative genomics of the HOG-signalling system in fungiMarcus Krantz
Department for Cell and Molecular Biology, , Box 462, , Sweden
Curr Genet 49:137-51. 2006..Protein size appears to be conserved largely independently from primary sequence and thus provides an additional tool for functional analysis and orthologue identification... - Yeast aquaglyceroporins use the transmembrane core to restrict glycerol transportCecilia Geijer
Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, S 40530 Gothenburg, Sweden
J Biol Chem 287:23562-70. 2012..This mechanism enables yeast cells to fine-tune intracellular glycerol levels at a wide range of extracellular osmolarities... - A regulatory domain in the C-terminal extension of the yeast glycerol channel Fps1pKristina Hedfalk
Department of Cell and Molecular Biology Microbiology, Gotegorg University, Götegorg, Sweden
J Biol Chem 279:14954-60. 2004..This element seems to be important for controlling Fps1p function. Similar to the N-terminal domain, the C-terminal domain is amphiphilic and has a potential to dip into the membrane... - Expression of the yeast aquaporin Aqy2 affects cell surface properties under the control of osmoregulatory and morphogenic signalling pathwaysKentaro Furukawa
Department of Cell and Molecular Biology Microbiology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden
Mol Microbiol 74:1272-86. 2009..Our observations suggest a potential link between aquaporins and cell surface properties, and relate to the proposed role of mammalian aquaporins in tumour cell migration and invasion... - Quantification of cell volume changes upon hyperosmotic stress in Saccharomyces cerevisiaeElzbieta Petelenz-Kurdziel
Department of Cell and Molecular Biology, University of Gothenburg, Medicinaregatan 9C, SE 413 90 Goteborg, Sweden
Integr Biol (Camb) 3:1120-6. 2011.... - Yeast osmoregulationStefan Hohmann
Department of Cell and Molecular Biology, Goteborg University, Goteborg, Sweden
Methods Enzymol 428:29-45. 2007..Simulations using mathematical models combined with time course measurements of different molecular processes in signaling and adaptation have allowed elucidation of the first system properties on the yeast osmoregulatory network... - Gis4, a new component of the ion homeostasis system in the yeast Saccharomyces cerevisiaeTian Ye
Department of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, S 40530 Göteborg, Sweden
Eukaryot Cell 5:1611-21. 2006..Hence, it appears that the conserved Snf1 kinase plays roles in different pathways controlling nutrient as well as stress response... - Conditional osmotic stress in yeast: a system to study transport through aquaglyceroporins and osmostress signalingSara Karlgren
Department of Cell and Molecular Biology, Goteborg University, S 405 30 Goteborg, Sweden
J Biol Chem 280:7186-93. 2005..Taken together, our "conditional" osmotic stress system facilitates studies on aquaglyceroporin function and reveals features of the osmosensing and signaling system... - Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiaeKarin Elbing
Department of Chemistry and Bioscience Molecular Biotechnology, Chalmers University of Technology, Box 462, SE 405 30 Goteborg, Sweden
Appl Environ Microbiol 70:5323-30. 2004..cerevisiae and will provide new tools for the control of industrial fermentations... - Aquaporins in yeasts and filamentous fungiNina Pettersson
Department of Cell and Molecular Biology, , Box 462, , Sweden
Biol Cell 97:487-500. 2005.... - Robustness and fragility in the yeast high osmolarity glycerol (HOG) signal-transduction pathwayMarcus Krantz
Department of Cell and Molecular Biology, University of Gothenburg, Goteborg, Sweden
Mol Syst Biol 5:281. 2009..Thus, in vivo robustness data can be used to discriminate and improve mathematical models... - Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiaeNina Pettersson
Department of Cell and Molecular Biology, , 40530, , Sweden
Curr Genet 50:247-55. 2006..We conclude that yeast is a versatile system for functional studies of aquaporins, and it can be developed to screen for compounds of potential pharmacological use... - Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiaeDominik Mojzita
Department of Cell and Molecular Biology Microbiology, Goteborg University, PO Box 462, 405 30, Goteborg, Sweden
Mol Genet Genomics 276:147-61. 2006..In conclusion, the THI regulon is the most interesting system to study principles of genes expression and metabolic coordination and deserves further attention... - Comparative analysis of HOG pathway proteins to generate hypotheses for functional analysisMarcus Krantz
Department for Cell and Molecular Biology, , Box 462, 40530, , Sweden
Curr Genet 49:152-65. 2006..We propose that any functional protein analysis in fungi should make use of the unique resource that fungal genome sequences offer... - Fungal fludioxonil sensitivity is diminished by a constitutively active form of the group III histidine kinaseKentaro Furukawa
Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden
FEBS Lett 586:2417-22. 2012..Our results explain the different reasons for fludioxonil resistance conferred by its deletion and missense mutation... - A framework for mapping, visualisation and automatic model creation of signal-transduction networksCarl Fredrik Tiger
Department of Cell and Molecular Biology, University of Gothenburg, Goteborg, Sweden
Mol Syst Biol 8:578. 2012..The framework is species independent and we expect that it will have wider impact in signalling research on any system... - Efficient construction of homozygous diploid strains identifies genes required for the hyper-filamentous phenotype in Saccharomyces cerevisiaeKentaro Furukawa
Department of Cell and Molecular Biology Microbiology, University of Gothenburg, Gothenburg, Sweden
PLoS ONE 6:e26584. 2011.... - The Ashbya gossypii EF-1α promoter of the ubiquitously used MX cassettes is toxic to Saccharomyces cerevisiaeRoja Babazadeh
Department of Cell and Molecular Biology, University of Gothenburg, Gothenburg, Sweden
FEBS Lett 585:3907-13. 2011..Toxicity could be eliminated by promoter replacement or exclusion of the marker cassette. To our knowledge, this is the first report of toxicity caused by introduction of a heterologous promoter alone... - Production, characterization and crystallization of the Plasmodium falciparum aquaporinKristina Hedfalk
Department of Chemistry and Bioscience Molecular Biotechnology, Chalmers University of Technology, Box 462, S 405 30 Goteborg, Sweden
Protein Expr Purif 59:69-78. 2008..pastoris (estimated to 64mg PfAQP per liter cell culture) PfAQP was purified to homogeneity (18mg/L) and initial attempts at crystallization of the protein yielded several different forms... - Osmotic adaptation in yeast--control of the yeast osmolyte systemStefan Hohmann
Department of Cell and Molecular Biology, , Sweden
Int Rev Cytol 215:149-87. 2002.... - A short regulatory domain restricts glycerol transport through yeast Fps1pMarkus J Tamás
Department of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, 40530 Goteborg, Sweden
J Biol Chem 278:6337-45. 2003.... - The Saccharomyces cerevisiae aquaporin Aqy1 is involved in sporulationFrédéric Sidoux-Walter
Department of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, S 40530 Göteborg, Sweden
Proc Natl Acad Sci U S A 101:17422-7. 2004..Taken together, we demonstrate a functional role of an aquaporin in gametogenesis, as well as in the formation of durable structures such as spores, a role that may have wider biological and medical implications... - Switching the mode of metabolism in the yeast Saccharomyces cerevisiaeKarin Otterstedt
Department of Chemistry and Bioscience Molecular Biotechnology, Chalmers University of Technology, Box 462, SE 405 30, Goteborg, Sweden
EMBO Rep 5:532-7. 2004..These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose-induced signal transduction... - Control of high osmolarity signalling in the yeast Saccharomyces cerevisiaeStefan Hohmann
Department of Cell and Molecular Biology, University of Gothenburg, Goteborg, Sweden
FEBS Lett 583:4025-9. 2009..These recent findings provide exciting insight into the higher control levels of signalling through this MAPK system of potential general importance... - Time course gene expression profiling of yeast spore germination reveals a network of transcription factors orchestrating the global responseCecilia Geijer
Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, Gothenburg, S 40530, Sweden
BMC Genomics 13:554. 2012.... - A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changesEmma Eriksson
Department of Physics, Goteborg University, SE 412 96, Goteborg, Sweden
Lab Chip 7:71-6. 2007..We intend to use the platform to study how the age of cells, their progression through the cell cycle, or their genetic landscape, alter their capacity (kinetics and amplitude) to respond to environmental changes... - Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiaeEva Albers
Department of Molecular Biotechnology, Chalmers University of Technology, Box 462, SE 405 30 Goteborg, Sweden
J Biol Chem 278:10264-72. 2003..Moreover, since phosphoglycerate dehydrogenase activity requires NAD(+) as cofactor, deletion of SER3 and SER33 markedly affected redox metabolism as shown by substrate and product analysis... - The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulationR Ansell
Department of General and Marine Microbiology, Gothenburg University, Sweden
EMBO J 16:2179-87. 1997..We conclude that expression of GPD2 is controlled by a novel, oxygen-independent, signalling pathway which is required to regulate metabolism under anoxic conditions... - The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stressA K Pahlman
Department of Cell and Molecular Biology, Microbiology, Goteborg University, Box 463, SE 40530 Goteborg, Sweden
J Biol Chem 276:3555-63. 2001..We conclude that glycerol metabolism plays multiple roles in yeast adaptation to altered growth conditions, explaining the complex regulation of glycerol biosynthesis genes... - Analysis of the pore of the unusual major intrinsic protein channel, yeast Fps1pR M Bill
Department of Cell and Molecular Biology Microbiology, Goteborg University, S 40530 Göteborg, Sweden
J Biol Chem 276:36543-9. 2001..We discuss the fact that this may accommodate the divergent NPA motifs of Fps1p and that the different pore structures of Fps1p and GlpF may reflect the physiological roles of the two glycerol facilitators... - Polymorphism of Saccharomyces cerevisiae aquaporinsV Laizé
Department of Cell and Molecular Biology, Microbiology, Goteborg University, Box 462, SE 40530 Goteborg, Sweden
Yeast 16:897-903. 2000..We conclude that natural and industrial conditions provide selective pressure to maintain AQY1 but apparently not AQY2... - Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrationsC Henricsson
Department of Chemistry and Bioscience-Molecular Biotechnology, Chalmers University of Technology, Box 462, , Sweden
Appl Environ Microbiol 71:6185-92. 2005.... - Transposon mutagenesis reveals novel loci affecting tolerance to salt stress and growth at low temperatureM C de Jesus Ferreira
Department of Cell and Molecular Biology/Microbiology, , Sweden
Curr Genet 40:27-39. 2001..htm) only marginally overlap. We discuss some of the features of transposon mutagenesis in light of the availability of the complete set of yeast deletion mutants and we discuss the possible roles of the genes we identified... - A simple mathematical model of adaptation to high osmolarity in yeastPeter Gennemark
Department of Computer Science and Engineering, Chalmers University of Technology, SE 412 96 Goteborg, Sweden
In Silico Biol 6:193-214. 2006..Besides, the low complexity makes it possible to obtain more reliable parameter estimates... - Combination of two activating mutations in one HOG1 gene forms hyperactive enzymes that induce growth arrestGilad Yaakov
Department of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Mol Cell Biol 23:4826-40. 2003..This study provides the first example of a MAPK that is intrinsically activated by mutations and induces a strong biological effect... - Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screenSara Karlgren
Department of Cell and Molecular Biology/Microbiology, , Sweden
Eur J Biochem 271:771-9. 2004..Our findings provide a framework for further genetic and structural analysis to better understand the mechanism that controls Fps1 function by osmotic changes... - Accumulation and release of the osmolyte glycerol is independent of the putative MIP channel Spac977.17p in Schizosaccharomyces pombeGerald Kayingo
Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
Antonie Van Leeuwenhoek 85:85-92. 2004..pombe appears distinct from that described in S. cerevisiae. Further studies are needed to elucidate the physiological role of the Spac977.17p channel... - Integrative model of the response of yeast to osmotic shockEdda Klipp
Berlin Center for Genome Based Bioinformatics, Max Planck Institute for Molecular Genetics, Dept Vertebrate Genomics, Ihnestr 73, 14195 Berlin, Germany
Nat Biotechnol 23:975-82. 2005..It also serves as a starting point for a comprehensive description of cellular signaling... - Aquaporin expression correlates with freeze tolerance in baker's yeast, and overexpression improves freeze tolerance in industrial strainsAn Tanghe
Laboratorium voor Moleculaire Celbiologie, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, B 3001 Leuven Heverlee, Flanders, Belgium
Appl Environ Microbiol 68:5981-9. 2002.... - [Systems biology can radically change health care. Basis for individualized prediction, prevention and treatment]Mikael Benson
Drottning Silvias barn- och ungdomssjukhus,
Lakartidningen 104:3037-41. 2007 - Yeast systems biology to unravel the network of lifeRoberta Mustacchi
Centre for Microbial Biotechnology CMB, Building 223, BioCentrum DTU, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
Yeast 23:227-38. 2006..This paper discusses the impacts of systems biology and how YSBN may play a role in the future development of the field... - Genome-wide expression profile of the mnn2 Delta mutant of Saccharomyces cerevisiaeIsaac Corbacho
Department of Microbiology, University of Extremadura, 06071, Badajoz, Spain
Antonie Van Leeuwenhoek 89:485-94. 2006..In addition, the mnn2 Delta strain seems to have a certain level of stimulation of DNA processing reactions while some genes involved in intracellular transport pathways are under-regulated... - Transcriptional responses to glucose at different glycolytic rates in Saccharomyces cerevisiaeKarin Elbing
Department of Chemistry and Bioscience Molecular Biotechnology, Chalmers University of Technology, Goteborg, Sweden
Eur J Biochem 271:4855-64. 2004..Taken together, it appears that glucose addition affects the expression of SUC2 as well as Mig1 activity by both Snf1-dependent and -independent mechanisms that can now be dissected and resolved as early and late/sustained responses... - Report of an EU projects workshop on systems biology held in Brussels, Belgium on 8 December 2004Ewan Birney
Syst Biol (Stevenage) 152:55-60. 2005 - The Pep4p vacuolar proteinase contributes to the turnover of oxidized proteins but PEP4 overexpression is not sufficient to increase chronological lifespan in Saccharomyces cerevisiaeMarta Marques
IBMC, Instituto de Biologia Molecular e Celular, Grupo de Microbiologia Celular e Aplicada, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
Microbiology 152:3595-605. 2006..These results indicate that Pep4p is important for protein turnover after oxidative damage; however, increased removal of oxidized proteins is not sufficient to enhance lifespan... - Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and apoptosisTeresa Almeida
IBMC, Instituto de Biologia Molecular e Celular, Grupo de Microbiologia Celular e Aplicada, 4150 180 Porto, Portugal
Mol Biol Cell 19:865-76. 2008..These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis...