Lisbeth Olsson

Summary

Affiliation: Chalmers University of Technology
Country: Sweden

Publications

  1. pmc Common and distant structural characteristics of feruloyl esterase families from Aspergillus oryzae
    D B R K Gupta Udatha
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden
    PLoS ONE 7:e39473. 2012
  2. pmc The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae
    Magnus Ask
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, SE 41296, Gothenburg, Sweden
    Biotechnol Biofuels 6:22. 2013
  3. pmc Simultaneous saccharification and co-fermentation for bioethanol production using corncobs at lab, PDU and demo scales
    Rakesh Koppram
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Goteborg SE 412 96, Sweden
    Biotechnol Biofuels 6:2. 2013
  4. pmc Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass
    Rakesh Koppram
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Goteborg, SE 412 96, Sweden
    Biotechnol Biofuels 5:32. 2012
  5. pmc Revealing the beneficial effect of protease supplementation to high gravity beer fermentations using "-omics" techniques
    Maya P Piddocke
    Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, DK 2800 Kongens Lyngby, Denmark
    Microb Cell Fact 10:27. 2011
  6. pmc A comparative summary of expression systems for the recombinant production of galactose oxidase
    Oliver Spadiut
    Division of Glycoscience, School of Biotechnology, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    Microb Cell Fact 9:68. 2010
  7. pmc The impact of phosphate scarcity on pharmaceutical protein production in S. cerevisiae: linking transcriptomic insights to phenotypic responses
    Ali Kazemi Seresht
    Protein Expression, Novo Nordisk A S, Novo Nordisk Park 1, 2760 Måløv, Denmark
    Microb Cell Fact 10:104. 2011
  8. pmc Studies of the production of fungal polyketides in Aspergillus nidulans by using systems biology tools
    Gianni Panagiotou
    Center for Microbial Biotechnology, Department of Systems Biology, Building 223, Technical University of Denmark, DK 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 75:2212-20. 2009
  9. ncbi request reprint Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae
    Vijayendran Raghevendran
    Center for Microbial Biotechnology, Building 223, BioCentrum DTU, Technical University of Denmark, DK 2800, Kongens Lyngby, Denmark
    J Biol Chem 281:12308-14. 2006
  10. doi request reprint Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations
    Peter Odman
    Department of Systems Biology, Technical University of Denmark, Building 223, DK 2800, Kgs Lyngby, Denmark
    Appl Microbiol Biotechnol 86:1745-59. 2010

Collaborators

Detail Information

Publications66

  1. pmc Common and distant structural characteristics of feruloyl esterase families from Aspergillus oryzae
    D B R K Gupta Udatha
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden
    PLoS ONE 7:e39473. 2012
    ..Following the increasing attention on these enzymes, a novel descriptor based classification system has been proposed for FAEs resulting into 12 distinct families and pharmacophore models for three FAE sub-families have been developed...
  2. pmc The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae
    Magnus Ask
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, SE 41296, Gothenburg, Sweden
    Biotechnol Biofuels 6:22. 2013
    ..In the present study, the effects of HMF and furfural on redox metabolism, energy metabolism and gene expression were investigated in anaerobic chemostats where the inhibitors were added to the feed-medium...
  3. pmc Simultaneous saccharification and co-fermentation for bioethanol production using corncobs at lab, PDU and demo scales
    Rakesh Koppram
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Goteborg SE 412 96, Sweden
    Biotechnol Biofuels 6:2. 2013
    ..In the current work, we designed a SSCF process and scaled up from lab to demo scale reaching 4% (w/v) ethanol using xylose rich corncobs...
  4. pmc Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass
    Rakesh Koppram
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Goteborg, SE 412 96, Sweden
    Biotechnol Biofuels 5:32. 2012
    ..abstract:..
  5. pmc Revealing the beneficial effect of protease supplementation to high gravity beer fermentations using "-omics" techniques
    Maya P Piddocke
    Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, DK 2800 Kongens Lyngby, Denmark
    Microb Cell Fact 10:27. 2011
    ....
  6. pmc A comparative summary of expression systems for the recombinant production of galactose oxidase
    Oliver Spadiut
    Division of Glycoscience, School of Biotechnology, Royal Institute of Technology KTH, SE 106 91 Stockholm, Sweden
    Microb Cell Fact 9:68. 2010
    ..graminearum, an optimized variant for E. coli and a codon-optimized gene for P. pastoris were expressed without the native pro-sequence, but with a His-tag either at the N- or the C-terminus of the enzyme...
  7. pmc The impact of phosphate scarcity on pharmaceutical protein production in S. cerevisiae: linking transcriptomic insights to phenotypic responses
    Ali Kazemi Seresht
    Protein Expression, Novo Nordisk A S, Novo Nordisk Park 1, 2760 Måløv, Denmark
    Microb Cell Fact 10:104. 2011
    ....
  8. pmc Studies of the production of fungal polyketides in Aspergillus nidulans by using systems biology tools
    Gianni Panagiotou
    Center for Microbial Biotechnology, Department of Systems Biology, Building 223, Technical University of Denmark, DK 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 75:2212-20. 2009
    ....
  9. ncbi request reprint Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae
    Vijayendran Raghevendran
    Center for Microbial Biotechnology, Building 223, BioCentrum DTU, Technical University of Denmark, DK 2800, Kongens Lyngby, Denmark
    J Biol Chem 281:12308-14. 2006
    ....
  10. doi request reprint Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations
    Peter Odman
    Department of Systems Biology, Technical University of Denmark, Building 223, DK 2800, Kgs Lyngby, Denmark
    Appl Microbiol Biotechnol 86:1745-59. 2010
    ..The analysis revealed four spectral profiles present in the fluorescence data, three of which were identified as pyridoxine, NAD(P)H, and flavin nucleotides, respectively...
  11. doi request reprint Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae
    Jin Hou
    Center for Microbial Biotechnology, Technical University of Denmark, Building 223, 2800 Kgs, Lyngby, Denmark
    Appl Microbiol Biotechnol 82:909-19. 2009
    ....
  12. doi request reprint Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum
    Kristian B R M Krogh
    Center for Microbial Biotechnology, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
    Appl Microbiol Biotechnol 86:143-54. 2010
    ..A novel assay was developed to characterize this glucose inhibition on cellobiose hydrolysis. The assay uses labelled glucose-13C6 as inhibitor and subsequent mass spectrometry analysis to quantify the hydrolysis rates...
  13. ncbi request reprint Manipulation of malic enzyme in Saccharomyces cerevisiae for increasing NADPH production capacity aerobically in different cellular compartments
    Margarida Moreira dos Santos
    Center for Process Biotechnology, Technical University of Denmark, BioCentrum DTU, Building 223, DK 2800 Lyngby, Denmark
    Metab Eng 6:352-63. 2004
    ..This may find application in redirecting fluxes for improving specific biotechnological applications...
  14. ncbi request reprint Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose
    Gianni Panagiotou
    Center for Microbial Biotechnology, Technical University of Denmark, BioCentrum DTU, Building 223, DK 2800 Kgs Lyngby, Denmark
    Metab Eng 8:474-82. 2006
    ..It was demonstrated that dissimilatory nitrate reduction allows F. oxysporum to exhibit typical respiratory metabolic behaviour with a highly active TCA cycle and a large demand for NADPH...
  15. pmc Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator
    Renata Usaite
    Department of Systems Biology, Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
    Mol Syst Biol 5:319. 2009
    ....
  16. doi request reprint Physiological characterization of brewer's yeast in high-gravity beer fermentations with glucose or maltose syrups as adjuncts
    Maya P Piddocke
    Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, 2800, Kongens Lyngby, Denmark
    Appl Microbiol Biotechnol 84:453-64. 2009
    ..Our study underlines the effects of the various stress factors on brewer's yeast metabolism and the influence of the type of sugar syrups on the fermentation performance and the flavor profile of the final beer...
  17. ncbi request reprint Intracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Soltofts Plads, Building 223, 2800 Lyngby, Denmark
    J Biotechnol 115:425-34. 2005
    ..On the other hand, the presence of high levels of gamma-amino-n-butyric acid (GABA) under anaerobic conditions suggests a functional GABA bypass and a possible block in the TCA cycle at these conditions...
  18. ncbi request reprint Penicillium brasilianum as an enzyme factory; the essential role of feruloyl esterases for the hydrolysis of the plant cell wall
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, 2800 Lyngby, Denmark
    J Biotechnol 130:219-28. 2007
    ..While total release of phenolic acids and pentoses was not observed, the synergistic enhancement of hydrolysis in the presence of feruloyl esterase was clearly demonstrated...
  19. doi request reprint The roles of galactitol, galactose-1-phosphate, and phosphoglucomutase in galactose-induced toxicity in Saccharomyces cerevisiae
    Willem A de Jongh
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, Søltofts Plads, DK 2800 Kgs Lyngby, Denmark
    Biotechnol Bioeng 101:317-26. 2008
    ....
  20. ncbi request reprint Production and partial characterization of arabinoxylan-degrading enzymes by Penicillium brasilianum under solid-state fermentation
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800, Kgs Lyngby, Denmark
    Appl Microbiol Biotechnol 72:1117-24. 2006
    ..The P. brasilianum CE produced in this work contains a balance of cell wall-modifying enzymes capable of degrading arabinoxylan of BSG by more than 40%...
  21. ncbi request reprint Steady-state and transient-state analyses of aerobic fermentation in Saccharomyces kluyveri
    Kasper Møller
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
    FEMS Yeast Res 2:233-44. 2002
    ..kluyveri led to ethanol formation after a delay of 20-50 min (depending on culture conditions prior to the pulse), which is in contrast to S. cerevisiae that ferments immediately after glucose addition...
  22. ncbi request reprint Ethanolic fermentation of acid pre-treated starch industry effluents by recombinant Saccharomyces cerevisiae strains
    Jesus Zaldivar
    Center for Microbial Biotechnology, Technical University of Denmark, BioCentrum DTU, Building 223, DK 2800 Kgs, Lyngby, Denmark
    Bioresour Technol 96:1670-6. 2005
    ..CPB.CR1 showed impaired growth compared to the two other xylose-utilizing strains, but displayed 18% increased ethanol yield in the post-fermentation effluent...
  23. doi request reprint On-line estimation of biomass, glucose and ethanol in Saccharomyces cerevisiae cultivations using in-situ multi-wavelength fluorescence and software sensors
    Peter Odman
    Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark
    J Biotechnol 144:102-12. 2009
    ..Corresponding numbers in low gravity models were 0.3 gl(-1) CDW, 0.7 gl(-1) ethanol and 1.0 gl(-1) consumed glucose, corresponding to 4%, 8% and 4% of the respective concentration intervals...
  24. ncbi request reprint Aerobic physiology of redox-engineered Saccharomyces cerevisiae strains modified in the ammonium assimilation for increased NADPH availability
    Margarida Moreira dos Santos
    Center for Process Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Lyngby, Denmark
    FEMS Yeast Res 4:59-68. 2003
    ..No redox effect was observed in a strain containing a deletion of GLR1, encoding glutathione reductase, an enzyme that is NADPH-consuming...
  25. ncbi request reprint A systems biology approach to study glucose repression in the yeast Saccharomyces cerevisiae
    Steen Lund Westergaard
    Center for Microbial Biotechnology, BioCentrum, Technical University of Denmark, Building 223, DK 2800 Kgs, Lyngby, Denmark
    Biotechnol Bioeng 96:134-45. 2007
    ....
  26. ncbi request reprint Dynamic effects related to steady-state multiplicity in continuous Saccharomyces cerevisiae cultivations
    Frede Lei
    CAPEC, Department of Chemical Engineering, Building 227, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Biotechnol Bioeng 88:838-48. 2004
    ..A more narrow multiplicity range, however, was obtained when the productostat was operated as an accelero-productostat...
  27. ncbi request reprint The influence of different cultivation conditions on the metabolome of Fusarium oxysporum
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    J Biotechnol 118:304-15. 2005
    ....
  28. ncbi request reprint Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains
    Thomas Grotkjaer
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Metab Eng 7:437-44. 2005
    ..CR4. Furthermore, the analysis indicated that the glyoxylate cycle was activated in CPB.CR4...
  29. pmc Industrial systems biology of Saccharomyces cerevisiae enables novel succinic acid cell factory
    Jose Manuel Otero
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
    PLoS ONE 8:e54144. 2013
    ..cerevisiae, and hence show proof of concept that this is a potentially attractive cell factory for over-producing different platform chemicals...
  30. pmc Systems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans
    Gianni Panagiotou
    Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark
    PLoS ONE 3:e3847. 2008
    ..niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity...
  31. doi request reprint Fermentation characteristics of Fusariumoxysporum grown on acetate
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Bioresour Technol 99:7397-401. 2008
    ....
  32. ncbi request reprint Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces cerevisiae improves growth and ethanol production on D-xylose
    Jennifer Headman Van Vleet
    Center for Microbial Biotechnology, Technical University of Denmark, BioCentrum DTU, Building 223, DK 2800 Kgs Lyngby, Denmark
    Metab Eng 10:360-9. 2008
    ..In addition to showing higher acetate levels, pho13Delta mutants also produced less glycerol on xylose, suggesting that deletion of Pho13p could improve growth by altering redox levels when cells are grown on xylose...
  33. ncbi request reprint Elucidation of the role of Grr1p in glucose sensing by Saccharomyces cerevisiae through genome-wide transcription analysis
    Steen L Westergaard
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, DK 2800 Kgs Lyngby, Denmark
    FEMS Yeast Res 5:193-204. 2004
    ....
  34. ncbi request reprint Growth and enzyme production by three Penicillium species on monosaccharides
    Henning Jørgensen
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, DK 2800 Kgs Lyngby, Denmark
    J Biotechnol 109:295-9. 2004
    ..After glucose depletion, the fungi started producing beta-glucosidase and endoglucanases. Xylose did not repress the enzyme production and it induced the production of endoxylanases and beta-xylosidases...
  35. pmc Identification of in vivo enzyme activities in the cometabolism of glucose and acetate by Saccharomyces cerevisiae by using 13C-labeled substrates
    Margarida Moreira dos Santos
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, DK 2800, Lyngby, Denmark
    Eukaryot Cell 2:599-608. 2003
    ..Cytoplasmic alanine aminotransferase activity was detected, and evidence was found that alpha-isopropylmalate synthase has two active forms in vivo, one mitochondrial and the other a short cytoplasmic form...
  36. ncbi request reprint Physiological characterization of glucose repression in the strains with SNF1 and SNF4 genes deleted
    Renata Usaite
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, DK 2800 Kgs Lyngby, Denmark
    J Biotechnol 133:73-81. 2008
    ..13 h(-1)) and were found to be 0.07, 0.08 and 0.04 h(-1) for the Deltasnf1, Deltasnf4 and Deltasnf1Deltasnf4 strains, respectively. The study showed that Snf1 kinase was not solely responsible for the derepression of galactose metabolism...
  37. pmc Characterization of global yeast quantitative proteome data generated from the wild-type and glucose repression saccharomyces cerevisiae strains: the comparison of two quantitative methods
    Renata Usaite
    BioCentrum DTU, Technical University of Denmark, Kgs Lyngby, Denmark
    J Proteome Res 7:266-75. 2008
    ..The functional categorization of the relative protein expression differences that occur in Snf1-deficient strains uncovers a wide range of biological processes regulated by this important cellular kinase...
  38. doi request reprint The interplay between sulphur and selenium metabolism influences the intracellular redox balance in Saccharomyces cerevisiae
    Valeria Mapelli
    Deparment of Chemical and Biological Engineering, Chalmers University of Technology, Goteborg, Sweden
    FEMS Yeast Res 12:20-32. 2012
    ....
  39. doi request reprint The challenge of improved secretory production of active pharmaceutical ingredients in Saccharomyces cerevisiae: a case study on human insulin analogs
    Ali Kazemi Seresht
    Industrial Biotechnology, Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
    Biotechnol Bioeng 110:2764-74. 2013
    ....
  40. doi request reprint Gene deletion of cytosolic ATP: citrate lyase leads to altered organic acid production in Aspergillus niger
    Susan Meijer
    Department for Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
    J Ind Microbiol Biotechnol 36:1275-80. 2009
    ..Genome-scale stoichiometric metabolic model predictions can be used for identifying gene targets. Deletion of the acl led to increased succinic acid production by A. niger...
  41. ncbi request reprint Physiological characterisation of acuB deletion in Aspergillus niger
    Susan Meijer
    Center for Microbial Biotechnology, DTU BioSys, Lyngby, Denmark
    Appl Microbiol Biotechnol 84:157-67. 2009
    ..The experiments demonstrated that also when acuB was deleted, no acetate was formed. Therefore, AcuB cannot be the only activator of AcuA, and another control mechanism has to be available for activating AcuA...
  42. pmc Improvement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: example of transcript analysis as a tool in inverse metabolic engineering
    Christoffer Bro
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 71:6465-72. 2005
    ..This conclusion was supported by measurements of sugar phosphates, which showed that there were increased concentrations of glucose-6-phosphate, galactose-6-phosphate, and fructose-6-phosphate in the strain construct overexpressing PGM2...
  43. ncbi request reprint Production of fungal alpha-amylase by Saccharomyces kluyveri in glucose-limited cultivations
    Kasper Møller
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, 2800 Kgs Lyngby, Denmark
    J Biotechnol 111:311-8. 2004
    ..kluyveri could be grown to high cell density, it was also observed that it has a high maintenance coefficient, which resulted in low biomass yields at the low specific growth rates prevailing towards the end of the fed-batch cultivation...
  44. pmc Combining substrate specificity analysis with support vector classifiers reveals feruloyl esterase as a phylogenetically informative protein group
    Roberto Olivares-Hernández
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
    PLoS ONE 5:e12781. 2010
    ..The diversity of substrate specificities found in the FAE family shows that this family is old enough to have experienced the emergence and loss of many activities...
  45. pmc Metabolic engineering of ammonium assimilation in xylose-fermenting Saccharomyces cerevisiae improves ethanol production
    Christophe Roca
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 69:4732-6. 2003
    ..Overexpression of the GS-GOGAT complex did not improve conversion of xylose to ethanol during batch cultivation, but it increased ethanol yield by 16% in carbon-limited continuous cultivation at a low dilution rate...
  46. pmc Identification of biomarkers for genotyping Aspergilli using non-linear methods for clustering and classification
    Irene Kouskoumvekaki
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    BMC Bioinformatics 9:59. 2008
    ....
  47. doi request reprint Overexpression of a novel endogenous NADH kinase in Aspergillus nidulans enhances growth
    Gianni Panagiotou
    Center for Microbial Biotechnology, Department of Systems Biology, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Metab Eng 11:31-9. 2009
    ..This conclusion may impact the design of new strains capable of generating reducing power in the form of NADPH, which is crucial for efficient production of many industrially important metabolites and enzymes...
  48. ncbi request reprint Chemometric analysis of in-line multi-wavelength fluorescence measurements obtained during cultivations with a lipase producing Aspergillus oryzae strain
    Martin B Haack
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Søltofts Plads, Building 223, DK 2800 Kgs Lyngby, Denmark
    Biotechnol Bioeng 96:904-13. 2007
    ..93. The results presented here clearly show that multi-wavelength fluorescence is a useful tool for monitoring fed-batch cultivations of filamentous fungi...
  49. ncbi request reprint Effect of compounds released during pretreatment of wheat straw on microbial growth and enzymatic hydrolysis rates
    Gianni Panagiotou
    Center for Microbial Biotechnology, BioCentrum DTU, Building 223, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Biotechnol Bioeng 96:250-8. 2007
    ..From all the potential inhibiting compounds that were tested, formic acid had the most severe influence on the hydrolysis rate resulting in a complete inactivation of the two enzyme mixtures...
  50. doi request reprint Evolutionary engineering of Saccharomyces cerevisiae for efficient aerobic xylose consumption
    Gionata Scalcinati
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
    FEMS Yeast Res 12:582-97. 2012
    ..The resulting strain is a desirable platform for the industrial production of biomass-related products using xylose as a sole carbon source...
  51. ncbi request reprint Experimental investigations of multiple steady states in aerobic continuous cultivations of Saccharomyces cerevisiae
    Frede Lei
    Computer Aided Process Engineering Center, Department of Chemical Engineering, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Biotechnol Bioeng 82:766-77. 2003
    ..However, at higher ethanol concentrations repression of the oxidative metabolism was observed (the oxidative capacity decreased), and this resulted in a broader range of dilution rates where multiple steady states could be found...
  52. ncbi request reprint Determination of cell mass and polymyxin using multi-wavelength fluorescence
    Anna Eliasson Lantz
    Center for Microbial Biotechnology, Building 223, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    J Biotechnol 121:544-54. 2006
    ..17+/-0.01 gg(-1), was reached in the cultivations where both nitrogen and phosphate concentrations were kept low...
  53. pmc Lipidomic profiling of Saccharomyces cerevisiae and Zygosaccharomyces bailii reveals critical changes in lipid composition in response to acetic acid stress
    Lina Lindberg
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden
    PLoS ONE 8:e73936. 2013
    ..The results also suggest that acetic acid tolerance is associated with the ability of a given strain to generate large rearrangements in its lipid profile. ..
  54. doi request reprint The interplay of descriptor-based computational analysis with pharmacophore modeling builds the basis for a novel classification scheme for feruloyl esterases
    D B R K Gupta Udatha
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Kemivagen 10, SE 412 96, Gothenburg, Sweden
    Biotechnol Adv 29:94-110. 2011
    ..Knowing the pharmacophoric features of a small molecule that are essential for binding to the members of a certain family opens a window of opportunities for tailored applications of FAEs...
  55. doi request reprint Long-term adaptation of Saccharomyces cerevisiae to the burden of recombinant insulin production
    Ali Kazemi Seresht
    Industrial Biotechnology, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivaegen 10, 41296, Gothenburg, Sweden
    Biotechnol Bioeng 110:2749-63. 2013
    ....
  56. pmc Engineering glutathione biosynthesis of Saccharomyces cerevisiae increases robustness to inhibitors in pretreated lignocellulosic materials
    Magnus Ask
    Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Gothenburg SE 41296, Sweden
    Microb Cell Fact 12:87. 2013
    ..In this study, Saccharomyces cerevisiae was engineered for increased robustness by modulating the redox state through overexpression of GSH1, CYS3 and GLR1, three genes involved in glutathione (GSH) metabolism...
  57. ncbi request reprint Fueling industrial biotechnology growth with bioethanol
    Jose Manuel Otero
    Center for Microbial Biotechnology, BioCentrum, Technical University of Denmark, BioCentrum DTU, 2800, Kgs Lyngby, Denmark
    Adv Biochem Eng Biotechnol 108:1-40. 2007
    ..Biofuel will continue to be the keystone of any industrial biotechnology-based economy whereby biorefineries leverage common raw materials and unit operations to integrate diverse processes to produce demand-driven product portfolios...
  58. ncbi request reprint Metabolic footprinting in microbiology: methods and applications in functional genomics and biotechnology
    Valeria Mapelli
    Department of Chemical and Biological Engineering, Chalmers University of Technology, SE 412 96 Gothenburg, Sweden
    Trends Biotechnol 26:490-7. 2008
    ..Hereby, we state that integrative information from metabolic footprinting can assist in further interpretation of metabolic networks...
  59. doi request reprint Metabolic and bioprocess engineering for production of selenized yeast with increased content of seleno-methylselenocysteine
    Valeria Mapelli
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Goteborg, Sweden
    Metab Eng 13:282-93. 2011
    ..Collectively, our data show how the combination of metabolic and bioprocess engineering can be used for the production of selenized yeast enriched with beneficial Se-metabolites...
  60. ncbi request reprint Change in hyphal morphology of Aspergillus oryzae during fed-batch cultivation
    Martin B Haack
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Søltofts Plads
    Appl Microbiol Biotechnol 70:482-7. 2006
    ..The results indicate that the two events are linked as a return to normal growth was observed upon cessation of production due to oxygen limitations...
  61. ncbi request reprint An expanded role for microbial physiology in metabolic engineering and functional genomics: moving towards systems biology
    Jens Nielsen
    Center for Process Biotechnology, Building 223, BioCentrum DTU, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    FEMS Yeast Res 2:175-81. 2002
    ..Clearly this will have a significant impact on microbial physiology as well as on metabolic engineering...
  62. ncbi request reprint On-line cell mass monitoring of Saccharomyces cerevisiae cultivations by multi-wavelength fluorescence
    Martin B Haack
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Søltofts Plads, Building 223, DK 2800 Kgs Lyngby, Denmark
    J Biotechnol 114:199-208. 2004
    ..20 and 0.19 g l(-1), respectively, when test set validation was used. Based on this work, it is evident that on-line monitoring of culture fluorescence can be used for estimation of the cell mass concentration during cultivations...
  63. ncbi request reprint The beta-subunits of the Snf1 kinase in Saccharomyces cerevisiae, Gal83 and Sip2, but not Sip1, are redundant in glucose derepression and regulation of sterol biosynthesis
    Jie Zhang
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivagen 10, Gothenburg, Sweden
    Mol Microbiol 77:371-83. 2010
    ..Furthermore, we found that Sip2, but not Sip1, can take over when Gal83 is deleted, but to a lesser extent. However, Sip1 may be sufficient for some other processes such as regulation of the nitrogen metabolism and meiosis...
  64. doi request reprint Lignocellulosic ethanol production at high-gravity: challenges and perspectives
    Rakesh Koppram
    Industrial Biotechnology, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goteborg SE 412 96, Sweden
    Trends Biotechnol 32:46-53. 2014
    ..Lignocellulosic materials add challenges compared to implemented processes due to high inhibitors content and the physical properties of these materials at high gravity. ..
  65. ncbi request reprint Screening genus Penicillium for producers of cellulolytic and xylanolytic enzymes
    Kristian B R Krogh
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, DK 2800 Lyngby, Denmark
    Appl Biochem Biotechnol 113:389-401. 2004
    ..The highest filter paper activity was measured after cultivation of Penicillium brasilianum IBT 20888 on cellulose...
  66. ncbi request reprint Aerobic glucose metabolism of Saccharomyces kluyveri: growth, metabolite production, and quantification of metabolic fluxes
    Kasper Møller
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
    Biotechnol Bioeng 77:186-93. 2002
    ..cerevisiae at the same growth conditions, where the tricarboxylic acid cycle operates as two branches. This indicates that the respiratory system was not significantly repressed in S. kluyveri during batch cultivation on glucose...