Nucleolar development and allocation of key nucleolar proteins require de novo transcription in bovine embryosOlga Svarcova
Department of Basic Animal and Veterinary Sciences, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
Mol Reprod Dev 74:1428-35. 2007
..However, localization of these two proteins to the nucleolar compartments was negatively affected by the alpha-amanitin treatment. Consequently, functional nucleoli were not established...
The role of RNA polymerase I transcription and embryonic genome activation in nucleolar development in bovine preimplantation embryosO Svarcova
IBHV, University of Copenhagen, Frederiksberg C, Denmark
Mol Reprod Dev 75:1095-103. 2008
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Nucleolar re-activation is delayed in mouse embryos cloned from two different cell linesO Svarcova
IBHV, University of Copenhagen, Frederiksberg C, Denmark
Mol Reprod Dev 76:132-41. 2009
..NT-MEF embryos displayed normal targeting but delayed activation of nucleolar proteins. Contrary, in NT-HM1 embryos, both of these processes were delayed...
Nuclear and nucleolar reprogramming during the first cell cycle in bovine nuclear transfer embryosOlga Østrup
Constantin the Philosopher University, Nitra, Slovak Republic
Cloning Stem Cells 11:367-75. 2009
..At 1-3 hpa, chromatin gradually decondensed toward the nucleus periphery and nuclear envelope reformed. From 4 hpa, the somatic cell nucleus gained a PN-like appearance and displayed NPBs suggesting ooplasmic control of development...
Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycleOlga Østrup
Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 7, Frederiksberg C, Denmark
Cell Reprogram 13:145-55. 2011
..In conclusion, the results demonstrate a possible reason why the intergeneric SCNT embryos never reached the full term...
Remodeling of ribosomal genes in somatic cells by Xenopus egg extractOlga Østrup
Institute of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark
Biochem Biophys Res Commun 412:487-93. 2011
..Condition-specific and rapid silencing of ribosomal genes may serve as a sensitive marker for evaluation of various reprogramming methods...
Ribosomal RNA and nucleolar proteins from the oocyte are to some degree used for embryonic nucleolar formation in cattle and pigP Maddox-Hyttel
Department of Animal and Veterinary Basic Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 7, DK 1870 Frederiksberg C, Denmark
Theriogenology 68:S63-70. 2007
..In conclusion, the nucleolus is important for the development of oocytes and embryos and may serve as a marker for the completion of oocyte growth and the normality of activation of the embryonic genome...