Olga Svarcova

Summary

Publications

  1. request reprint
    Svarcova O, Laurincik J, Avery B, Mlyncek M, Niemann H, Maddox Hyttel P. Nucleolar development and allocation of key nucleolar proteins require de novo transcription in bovine embryos. Mol Reprod Dev. 2007;74:1428-35 pubmed
    ..However, localization of these two proteins to the nucleolar compartments was negatively affected by the alpha-amanitin treatment. Consequently, functional nucleoli were not established...
  2. Østrup O, Petrovicova I, Strejcek F, Morovic M, Lucas Hahn A, Lemme E, et al. Nuclear and nucleolar reprogramming during the first cell cycle in bovine nuclear transfer embryos. Cloning Stem Cells. 2009;11:367-75 pubmed publisher
    ..At 1-3 hpa, chromatin gradually decondensed toward the nucleus periphery and nuclear envelope reformed. From 4 hpa, the somatic cell nucleus gained a PN-like appearance and displayed NPBs suggesting ooplasmic control of development. ..
  3. Svarcova O, Dinnyes A, Polgar Z, Bodo S, Adorjan M, Meng Q, et al. Nucleolar re-activation is delayed in mouse embryos cloned from two different cell lines. Mol Reprod Dev. 2009;76:132-41 pubmed publisher
    ..NT-MEF embryos displayed normal targeting but delayed activation of nucleolar proteins. Contrary, in NT-HM1 embryos, both of these processes were delayed. ..
  4. Østrup O, Strejcek F, Petrovicova I, Lucas Hahn A, Morovic M, Lemme E, et al. Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle. Cell Reprogram. 2011;13:145-55 pubmed publisher
    ..In conclusion, the results demonstrate a possible reason why the intergeneric SCNT embryos never reached the full term. ..
  5. Østrup O, Hyttel P, Klærke D, Collas P. Remodeling of ribosomal genes in somatic cells by Xenopus egg extract. Biochem Biophys Res Commun. 2011;412:487-93 pubmed publisher
    ..Condition-specific and rapid silencing of ribosomal genes may serve as a sensitive marker for evaluation of various reprogramming methods. ..
  6. Østrup O, Pedersen H, Holm H, Hyttel P. Analysis of nucleolar morphology and protein localization as an indicator of nuclear reprogramming. Methods Mol Biol. 2015;1222:161-74 pubmed publisher
    ..Moreover, detailed protocols for monitoring the nucleolar changes by transmission electron microscopy and immunocytochemistry are presented. ..
  7. Svarcova O, Strejcek F, Petrovicova I, Avery B, Pedersen H, Lucas Hahn A, et al. The role of RNA polymerase I transcription and embryonic genome activation in nucleolar development in bovine preimplantation embryos. Mol Reprod Dev. 2008;75:1095-103 pubmed publisher
  8. Ostrup O, Reiner A, Aleström P, Collas P. The specific alteration of histone methylation profiles by DZNep during early zebrafish development. Biochim Biophys Acta. 2014;1839:1307-15 pubmed publisher
    ..Altered H3K9me3 profiles were associated with severe neuronal and cranial phenotypes at day 4-5 post-fertilization. Thus, DZNep was shown to affect enrichment patterns of H3K27me3 and H3K9me3 at promoters in a gene-specific manner. ..