Stolfi A, Gandhi S, Salek F, Christiaen L. Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014;141:4115-20 pubmed publisher
..We demonstrate that CRISPR/Cas9 is sufficiently effective and specific to generate large numbers of embryos carrying mutations in a targeted gene of interest, which should allow for rapid screening of gene function in Ciona. ..
Stolfi A, Wagner E, Taliaferro J, Chou S, Levine M. Neural tube patterning by Ephrin, FGF and Notch signaling relays. Development. 2011;138:5429-39 pubmed publisher
..This MG regulatory network provides a foundation for elucidating the genetic and cellular basis of a model chordate central pattern generator...
Stolfi A, Christiaen L. Genetic and genomic toolbox of the chordate Ciona intestinalis. Genetics. 2012;192:55-66 pubmed publisher
..The genomic resources available will continue to support the design and interpretation of genetic experiments and allow for increasingly sophisticated approaches on a high-throughput, whole-genome scale...