PNA for rapid microbiologyHenrik Stender
Boston Probes, Inc, 15 DeAngelo Drive, Bedford, MA 01730, USA
J Microbiol Methods 48:1-17. 2002
....
PNA fluorescent in situ hybridization for rapid microbiology and cytogenetic analysisBrett Williams
Applied Biosystems, Bedford, MA, USA
Methods Mol Biol 208:181-93. 2002
PNA FISH: an intelligent stain for rapid diagnosis of infectious diseasesHenrik Stender
AdvanDx, Inc, 25K Olympia Avenue, Woburn, MA 01801, USA
Expert Rev Mol Diagn 3:649-55. 2003
..Having transitioned from an academic curiosity into an advanced diagnostic tool, PNA probes are now debuting on the infectious disease stage, representing the new generation of therapy-directing diagnostics...
Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlowJAN TRNOVSKY
AdvanDx, Inc, 10A Roessler Road, Woburn, MA 01801, USA
J Clin Microbiol 46:1537-40. 2008
..This method facilitates the accurate identification of clinical yeast isolates using two scoring techniques: flow cytometry and fluorescence microscopy...
Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probesKenneth Oliveira
Boston Probes, Inc, Bedford, Massachusetts 01730, USA
J Clin Microbiol 40:247-51. 2002
..It is concluded that this rapid method (2.5 h) for identification of S. aureus directly from blood culture bottles that contain GPCC offers important information for optimal antibiotic therapy...
Evaluation of mupA EVIGENE assay for determination of high-level mupirocin resistance in Staphylococcus aureusAnne K I Rasmussen
AdvanDx, Bygstubben 11, Vedbaek 2950, Denmark
J Clin Microbiol 48:4253-5. 2010
..aureus (MRSA). The aim of this study was to determine the presence of high-level mupirocin resistance by a new commercially available mupA genotypic diagnostic product, mupA EVIGENE assay (AdvanDx)...
Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottlesJaneen R Shepard
AdvanDx, Inc, 10A Roessler Road, Woburn, MA 01801, USA
J Clin Microbiol 46:50-5. 2008
..albicans and C. glabrata in positive blood culture bottles. This rapid assay may aid in the selection of initial antifungal drugs, leading to improved patient outcomes...
Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometryHanna Hartmann
, , Germany
J Clin Microbiol 43:4855-7. 2005
..We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures...
Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood culturesMette Søgaard
Department of Clinical Microbiology, Aalborg Hospital, Aarhus University Hospital, Postbox 365, 9100 Aalborg, Denmark
J Med Microbiol 56:914-7. 2007
..Discrepancies were resolved by PNA FISH retest and phenotypic tests. In conclusion, the K. pneumoniae probe provided an accurate diagnosis within 3 h and may supplement other methods for direct identification of Gram-negative bacteria...
Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottlesSusan Rigby
Applied Biosystems, Bedford, Massachusetts 01730, USA
J Clin Microbiol 40:2182-6. 2002
..It is concluded that this 2.5-h method for the definitive identification of C. albicans directly from yeast-positive blood culture bottles provides important information for optimal antifungal therapy and patient management...
Direct identification of Staphylococcus aureus from positive blood culture bottlesKenneth Oliveira
Applied Biosystems, Bedford, Boston, Massachusetts, USA
J Clin Microbiol 41:889-91. 2003
..1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded that S. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems...