C Vilches

Summary

Country: Spain

Publications

  1. ncbi request reprint Complete coding sequences and haplotypic associations of HLA-B*0707, -B*1524, -B*4405, -B*4802, -DRB1*0409, -DRB1*0411, -DRB1*1115, -DRB1*1305, and the novel allele -DRB1*0709. Group-specific amplification of cDNA from DRB1 alleles associated to DRB3 and
    C Vilches
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, 28035 Madrid, Spain
    Tissue Antigens 65:529-38. 2005
  2. ncbi request reprint KIR: diverse, rapidly evolving receptors of innate and adaptive immunity
    Carlos Vilches
    Servicio de Inmunologia, Hospital Universitario Clinica Puerta de Hierro, San Martin de Porres 4, 28035 Madrid, Spain
    Annu Rev Immunol 20:217-51. 2002
  3. ncbi request reprint HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea)
    R de Pablo
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 50:593-601. 1997
  4. ncbi request reprint The novel HLA-Cw*1802 allele is associated with B*5703 in the Bubi population from Equatorial Guinea
    C Vilches
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 49:644-8. 1997
  5. ncbi request reprint Complementary DNA sequence of the HLA-B*3924 allele
    E Estefanía
    Servicio de Immunologia, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Eur J Immunogenet 30:11-2. 2003
  6. ncbi request reprint Specific amplification of the HLA-DRB4 gene from c-DNA. Complete coding sequence of the HLA alleles DRB4*0103101 and DRB4*01033
    R de Pablo
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 59:44-6. 2002
  7. doi request reprint A method for simple and accurate identification of the multiple sclerosis associated allele HLA-DRB1*1501 in neuroscience research laboratories
    E Cisneros
    Inmunogenética HLA, Hospital Universitario Puerta de Hierro, Majadahonda, Spain
    J Neuroimmunol 225:143-8. 2010
  8. ncbi request reprint A new pair of HLA-C alleles, Cw*12042 and Cw*1203, differing at the KIR-related dimorphism of codons 77-80
    C Vilches
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 51:101-5. 1998
  9. ncbi request reprint Complete coding regions of two novel HLA-B alleles detected by phototyping (PCR-SSP) in the British caucasoid population: B*5108 and B*5002
    C Vilches
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 50:38-41. 1997
  10. doi request reprint The cannabinoid receptor 1 gene (CNR1) and multiple sclerosis: an association study in two case-control groups from Spain
    E Ramil
    Neuroinmunology Unit, Hospital Universitario Puerta de Hierro, Majadahonda, Spain
    Mult Scler 16:139-46. 2010

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Complete coding sequences and haplotypic associations of HLA-B*0707, -B*1524, -B*4405, -B*4802, -DRB1*0409, -DRB1*0411, -DRB1*1115, -DRB1*1305, and the novel allele -DRB1*0709. Group-specific amplification of cDNA from DRB1 alleles associated to DRB3 and
    C Vilches
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, 28035 Madrid, Spain
    Tissue Antigens 65:529-38. 2005
    ..The primary structures, functional features, evolutionary relationships, haplotypic associations, and population distributions of each of the nine HLA-B and -DRB1 alleles reported here are reviewed...
  2. ncbi request reprint KIR: diverse, rapidly evolving receptors of innate and adaptive immunity
    Carlos Vilches
    Servicio de Inmunologia, Hospital Universitario Clinica Puerta de Hierro, San Martin de Porres 4, 28035 Madrid, Spain
    Annu Rev Immunol 20:217-51. 2002
    ..This diversity likely contributes variability to the function of NK cells and T-lymphocytes by modulating innate and adaptive immune responses to specific challenges...
  3. ncbi request reprint HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea)
    R de Pablo
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 50:593-601. 1997
    ..The most frequent seven-locus haplotype is: A*30-Cw*17-B*42-DRB1*1102-DRB3*0202-DQA1*05-DQB1 *0301. In terms of genetic distance, the Bubi are closer to other Bantu groups than to West African populations...
  4. ncbi request reprint The novel HLA-Cw*1802 allele is associated with B*5703 in the Bubi population from Equatorial Guinea
    C Vilches
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 49:644-8. 1997
    ..In this study, the new Cw*1802 variant, differing from Cw*1801 at exon 5, is found associated with B*5703 in Bubi individuals from Equatorial Guinea. Confirmatory complete coding regions of B*5703 and B*3910 are also reported...
  5. ncbi request reprint Complementary DNA sequence of the HLA-B*3924 allele
    E Estefanía
    Servicio de Immunologia, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Eur J Immunogenet 30:11-2. 2003
    ..A single amino acid change exclusive to B*3924 (threonine-98) distinguishes it from B*3903...
  6. ncbi request reprint Specific amplification of the HLA-DRB4 gene from c-DNA. Complete coding sequence of the HLA alleles DRB4*0103101 and DRB4*01033
    R de Pablo
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 59:44-6. 2002
    ..The phenotype of G081 (A*01; B*0702, *1302/1303; Cw*0602, *07; DRB1*0403, *0701; DRB4*0103101, *01033; DQB1*0202, *0302; DQA1*0201, *0301) is consistent with a proposed association of DRB4*01033 with DRB1*0403 and DQB1*0302...
  7. doi request reprint A method for simple and accurate identification of the multiple sclerosis associated allele HLA-DRB1*1501 in neuroscience research laboratories
    E Cisneros
    Inmunogenética HLA, Hospital Universitario Puerta de Hierro, Majadahonda, Spain
    J Neuroimmunol 225:143-8. 2010
    ..This approach, much simpler than previously available methods, should facilitate research on MS by making accurate identification of DRB1*1501 accessible to neuroscience laboratories...
  8. ncbi request reprint A new pair of HLA-C alleles, Cw*12042 and Cw*1203, differing at the KIR-related dimorphism of codons 77-80
    C Vilches
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 51:101-5. 1998
    ....
  9. ncbi request reprint Complete coding regions of two novel HLA-B alleles detected by phototyping (PCR-SSP) in the British caucasoid population: B*5108 and B*5002
    C Vilches
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 50:38-41. 1997
    ..The primary structure of B*5002, intermediate between those of B*4501 and B*5001, raises further concern about the current classification of B*45 as a B12 rather than as a B*50 subtype...
  10. doi request reprint The cannabinoid receptor 1 gene (CNR1) and multiple sclerosis: an association study in two case-control groups from Spain
    E Ramil
    Neuroinmunology Unit, Hospital Universitario Puerta de Hierro, Majadahonda, Spain
    Mult Scler 16:139-46. 2010
    ..Disease severity and progression was unrelated to AAT repeat variations. In conclusion, long (AAT) > or = (13) CNR1 genotypes could behave as risk factors for PPMS...
  11. doi request reprint Allele-specific amplification of the complete HLA-C gene from genomic DNA - a novel Cw4 allele (C*04:71) with a Cw1 motif in the peptide-binding site
    M Moraru
    Laboratorio de Inmunogenética HLA, Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, Spain
    Tissue Antigens 79:291-4. 2012
    ..The principle we have used for C*04:71 isolation could be adapted for unambiguous sequence-based HLA-C typing of selected samples in a clinical setting...
  12. doi request reprint Molecular characterisation of KIR2DS2*005, a fusion gene associated with a shortened KIR haplotype
    D Ordonez
    Inmunogenética HLA, Hospital Universitario Puerta de Hierro, Majadahonda, Spain
    Genes Immun 12:544-51. 2011
    ..2% of Caucasoids. Using a combination of two monoclonal antibodies, we also demonstrate that KIR2DS2*005 encodes a molecule expressed on the surface of natural killer- and T-lymphocytes...
  13. ncbi request reprint Influence of KIR gene diversity on the course of HSV-1 infection: resistance to the disease is associated with the absence of KIR2DL2 and KIR2DS2
    E Estefanía
    Department of Immunology, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, San Martin de Porres 4, 28035 Madrid, Spain
    Tissue Antigens 70:34-41. 2007
    ....
  14. doi request reprint Simple genotyping of functional polymorphisms of the human immunoglobulin G receptors CD16A and CD32A: a reference cell panel
    C Vilches
    Department of Immunology, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Tissue Antigens 71:242-6. 2008
    ..We also present the CD16A and CD32A genotypes of cells from the Tenth International Histocompatibility Workshop, which can serve as a reference cell panel for investigating the influence of CD16A and CD32A polymorphisms on human health...
  15. ncbi request reprint A new HLA-Cw*15 allele, Cw*1508, identified in the Peruvian population
    L Sanz
    Servicio de Inmunologia, Clinica Puerto de Hierro, Madrid, Spain
    Tissue Antigens 53:391-3. 1999
    ..The nucleotide sequence of Cw*1508 only differs from that of Cw*1502 at position 539; this change determines the replacement of Leu by Arg 156 in Cw*1508...
  16. ncbi request reprint The complete primary structure of Cw*1701 reveals a highly divergent HLA class I molecule
    M J Herrero
    Servicio de Inmunologia, Clinica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 49:267-70. 1997
    ..Cw*1701 is thus a most unusual HLA-C molecule defining a third allelic lineage of this locus...
  17. doi request reprint Assessment of copy-number variation in the NKG2C receptor gene in a single-tube and characterization of a reference cell panel, using standard polymerase chain reaction
    M Moraru
    Laboratorio de Inmunogenética HLA, Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, Spain
    Tissue Antigens 80:184-7. 2012
    ..Our results should facilitate research on the importance of NKG2C and its deletion for immunity...
  18. doi request reprint Multiple sclerosis associates with LILRA3 deletion in Spanish patients
    D Ordonez
    Inmunogenética HLA, Hospital Universitario Puerta de Hierro, 28220 Majadahonda, Spain
    Genes Immun 10:579-85. 2009
    ..The relationship of LILRA3 deletion with R-MS is not secondary to linkage disequilibrium with a KIR gene, but we cannot exclude some contributions of KIR to the genetic susceptibility to R-MS...
  19. doi request reprint Duplication, mutation and recombination of the human orphan gene KIR2DS3 contribute to the diversity of KIR haplotypes
    D Ordonez
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Genes Immun 9:431-7. 2008
    ..Our results help understand the genetics of KIR2DS3 and the diversity of human KIR genotypes...
  20. ncbi request reprint Facilitation of KIR genotyping by a PCR-SSP method that amplifies short DNA fragments
    C Vilches
    Department of Immunology, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Tissue Antigens 70:415-22. 2007
    ..In addition, the new PCR-SSP method detects recently described alleles of several KIR genes, and allows for discrimination between the major structural variants of KIR2DS4 and KIR3DP1 without increasing the number of reactions...
  21. doi request reprint The 5' intergenic, promoter, pseudoexon 3 and complete coding sequences of the hybrid gene KIR2DS3*002
    D Ordonez
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Tissue Antigens 72:504-5. 2008
    ..Genomic and mRNA sequences support the KIR2DS3*002 gene being a hybrid of KIR2DS3*00103 and KIR2DS5...
  22. ncbi request reprint An apparent KIR2DS2-negative KIR2DL2-positive genotype discloses the novel allele KIR2DS2*00104
    N Gómez-Lozano
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, San Martin de Porres 4, 28035 Madrid, Spain
    Tissue Antigens 70:350-1. 2007
    ..KIR2DS2*00104 lacks a distinctive synonymous substitution of KIR2DS2 in nucleotide 418 that affects KIR genotyping...
  23. ncbi request reprint Point mutation of an EYA1-gene splice site in a patient with oto-facio-cervical syndrome
    E Estefanía
    Servicio de Inmunologia, ENT Department, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, Spain
    Ann Hum Genet 70:140-4. 2006
    ..Our results indicate that not only major rearrangements, but also point mutations altering the EYA1 reading frame, can be found in patients with OFC syndrome...
  24. ncbi request reprint HLA-B73: an atypical HLA-B molecule carrying a Bw6-epitope motif variant and a B pocket identical to HLA-B27
    C Vilches
    Servicio de Inmunologia, Hospital Puerta de Hierro, Madrid, Spain
    Immunogenetics 40:166. 1994
  25. ncbi request reprint Group-specific amplification of cDNA from DRB1 genes. Complete coding sequences of partially defined alleles and identification of the new alleles DRB1*040602, DRB1*111102, DRB1*080103, and DRB1*0113
    Antonio Balas
    Departamento de Histocompatibilidad, Centro de Transfusión de la Comunidad de Madrid, Madrid, Spain
    Hum Immunol 67:1008-16. 2006
    ..In addition, we found a novel allele, DRB1*0113, whose second exon carries a sequence motif characteristic of DRB1*07 alleles. The predicted class II haplotypic associations of all alleles are reported and discussed...
  26. ncbi request reprint Complementary DNA sequence of the novel HLA-B*3704 allele
    E Estefanía
    Servicio de Inmunologia, H U Clínica Puerta de Hierro, Madrid, Spain
    Tissue Antigens 59:142-4. 2002
    ..Therefore, the His171 substitution seen in HLA-B*3704 is likely to affect its antigen-presenting properties and is probably responsible for the differentiated serological phenotype of this allele in comparison with B*3701...
  27. ncbi request reprint Genotyping of human killer-cell immunoglobulin-like receptor genes by polymerase chain reaction with sequence-specific primers: an update
    N Gómez-Lozano
    Servicio de Inmunologia, H U Clinica Pueta de Hierro, Madrid, Spain
    Tissue Antigens 59:184-93. 2002
    ..Furthermore, an additional set of six reactions permits subtyping of KIR2DL5 variants, each of which shows well-differentiated functional and genetic features...
  28. ncbi request reprint Some human KIR haplotypes contain two KIR2DL5 genes: KIR2DL5A and KIR2DL5B
    Natalia Gómez-Lozano
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, San Martin de Porres 4, 28035 Madrid, Spain
    Immunogenetics 54:314-9. 2002
    ..These results are consistent with a model in which KIR2DL5A and KIR2DL5B are products of a gene duplication, which through the action of subsequent recombination have became separated on some haplotypes...
  29. ncbi request reprint Recognition of HLA-G by the NK cell receptor KIR2DL4 is not essential for human reproduction
    Natalia Gómez-Lozano
    Inmunologia, Hospital Universitario Puerta de Hierro, San Martin de Porres 4, E 28035 Madrid, Spain
    Eur J Immunol 33:639-44. 2003
    ..However, we demonstrate here that the KIR2DL4:HLA-G interaction is not essential for human reproduction by showing that a multiparous woman lacks a KIR2DL4 gene...
  30. ncbi request reprint Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002
    Steven G E Marsh
    Anthony Nolan Research Institute, Royal Free Hospital, Hampstead, London, United Kingdom
    Hum Immunol 64:648-54. 2003
  31. ncbi request reprint Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002
    Steven G E Marsh
    Anthony Nolan Research Institute, Royal Free Hospital, Pond Street, Hampstead, London NW3 2QG, UK
    Immunogenetics 55:220-6. 2003
  32. ncbi request reprint Imprint of human cytomegalovirus infection on the NK cell receptor repertoire
    Monica Guma
    Molecular Immunopathology Unit, DCEXS, Universitat Pompeu Fabra, Barcelona, Spain
    Blood 104:3664-71. 2004
    ..Altogether, these data support that HCMV infection selectively shapes the natural killer cell receptor (NKR) repertoire of NK and T cells from healthy carrier individuals...
  33. ncbi request reprint The silent KIR3DP1 gene (CD158c) is transcribed and might encode a secreted receptor in a minority of humans, in whom the KIR3DP1, KIR2DL4 and KIR3DL1/KIR3DS1 genes are duplicated
    Natalia Gómez-Lozano
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Eur J Immunol 35:16-24. 2005
    ..The putative KIR3DP1*004 product, like those of the related genes LAIR-2 and LILRA3/ILT6/LIR4, is predicted to be secreted to the extracellular medium rather than anchored to the cell membrane...
  34. ncbi request reprint Do NK-cell receptors and alloreactivity affect solid organ transplantation?
    Carlos Vilches
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, San Martin de Porres 4, 28035 Madrid, Spain
    Transpl Immunol 17:27-30. 2006
    ..These receptors could represent intrinsic patient factors that influence the success of their transplanted solid organs...
  35. ncbi request reprint Epigenetic silencing of potentially functional KIR2DL5 alleles: Implications for the acquisition of KIR repertoires by NK cells
    Natalia Gómez-Lozano
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, Madrid, Spain
    Eur J Immunol 37:1954-65. 2007
    ..Our results are consistent with a model in which RUNX factors could function as switch elements in the acquisition of KIR repertoires by NK cell precursors...
  36. ncbi request reprint Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets
    Ernesto Estefanía
    Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, San Martin de Porres 4, 28035 Madrid, Spain
    J Immunol 178:4402-10. 2007
    ..KIR2DL5 is thus an inhibitory receptor gathering a combination of genetic, structural, and functional features unique among KIR, which suggests that KIR2DL5 plays a specialized role in innate immunity...
  37. ncbi request reprint MHC class I peptide binding and tapasin
    Carlos Vilches
    J Immunol 171:3. 2003
  38. ncbi request reprint Three structurally and functionally divergent kinds of promoters regulate expression of clonally distributed killer cell Ig-like receptors (KIR), of KIR2DL4, and of KIR3DL3
    Hans Ingo Trompeter
    Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University, Dusseldorf, Germany
    J Immunol 174:4135-43. 2005
    ..The present study suggests that AML-2 acts as a repressor of KIR expression in mature NK cells and opens the possibility that AML factors and associated cofactors are involved in regulation of KIR expression during NK cell development...