Juan A Rosado

Summary

Affiliation: University of Extremadura
Country: Spain

Publications

  1. ncbi Early caspase-3 activation independent of apoptosis is required for cellular function
    Juan A Rosado
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, Caceres, Spain
    J Cell Physiol 209:142-52. 2006
  2. doi Dynamic interaction of hTRPC6 with the Orai1-STIM1 complex or hTRPC3 mediates its role in capacitative or non-capacitative Ca(2+) entry pathways
    Isaac Jardin
    Department of Physiology, University of Extremadura, Caceres, Spain
    Biochem J 420:267-76. 2009
  3. ncbi Two pathways for store-mediated calcium entry differentially dependent on the actin cytoskeleton in human platelets
    Juan A Rosado
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    J Biol Chem 279:29231-5. 2004
  4. doi Capacitative and non-capacitative signaling complexes in human platelets
    Alejandro Berna-Erro
    Department of Physiology, University of Extremadura, Caceres, Spain
    Biochim Biophys Acta 1823:1242-51. 2012
  5. doi Melatonin reduces apoptosis induced by calcium signaling in human leukocytes: Evidence for the involvement of mitochondria and Bax activation
    Javier Espino
    Department of Physiology, University of Extremadura, Badajoz, Spain
    J Membr Biol 233:105-18. 2010
  6. doi Transient receptor potential ankyrin-1 (TRPA1) modulates store-operated Ca(2+) entry by regulation of STIM1-Orai1 association
    Letizia Albarrán
    Department of Physiology Cell Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Biochim Biophys Acta 1833:3025-34. 2013
  7. ncbi Endogenously generated reactive oxygen species reduce PMCA activity in platelets from patients with non-insulin-dependent diabetes mellitus
    Isaac Jardin
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Platelets 17:283-8. 2006
  8. doi Homers regulate calcium entry and aggregation in human platelets: a role for Homers in the association between STIM1 and Orai1
    Isaac Jardin
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Biochem J 445:29-38. 2012
  9. doi Store-operated Ca2+ entry is sensitive to the extracellular Ca2+ concentration through plasma membrane STIM1
    Isaac Jardin
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Biochim Biophys Acta 1793:1614-22. 2009
  10. doi Orai1 mediates the interaction between STIM1 and hTRPC1 and regulates the mode of activation of hTRPC1-forming Ca2+ channels
    Isaac Jardin
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    J Biol Chem 283:25296-304. 2008

Collaborators

Detail Information

Publications88

  1. ncbi Early caspase-3 activation independent of apoptosis is required for cellular function
    Juan A Rosado
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, Caceres, Spain
    J Cell Physiol 209:142-52. 2006
    ..Thus, early activation of caspase-3 seems to be a non-apoptotic event required for cellular function...
  2. doi Dynamic interaction of hTRPC6 with the Orai1-STIM1 complex or hTRPC3 mediates its role in capacitative or non-capacitative Ca(2+) entry pathways
    Isaac Jardin
    Department of Physiology, University of Extremadura, Caceres, Spain
    Biochem J 420:267-76. 2009
    ..These findings support the hypothesis that hTRPC6 participates both in CCE and NCCE through its interaction with the Orai1-STIM1 complex or hTRPC3 respectively...
  3. ncbi Two pathways for store-mediated calcium entry differentially dependent on the actin cytoskeleton in human platelets
    Juan A Rosado
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    J Biol Chem 279:29231-5. 2004
    ....
  4. doi Capacitative and non-capacitative signaling complexes in human platelets
    Alejandro Berna-Erro
    Department of Physiology, University of Extremadura, Caceres, Spain
    Biochim Biophys Acta 1823:1242-51. 2012
    ....
  5. doi Melatonin reduces apoptosis induced by calcium signaling in human leukocytes: Evidence for the involvement of mitochondria and Bax activation
    Javier Espino
    Department of Physiology, University of Extremadura, Badajoz, Spain
    J Membr Biol 233:105-18. 2010
    ..Our results suggest that melatonin reduces caspase-9 and -3 activities induced by increases in [Ca(2+)]( c ) in human leukocytes, which are produced through the inhibition of both mPTP and Bax activation...
  6. doi Transient receptor potential ankyrin-1 (TRPA1) modulates store-operated Ca(2+) entry by regulation of STIM1-Orai1 association
    Letizia Albarrán
    Department of Physiology Cell Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Biochim Biophys Acta 1833:3025-34. 2013
    ..Altogether, these findings provide evidence for a new and interesting function of TRPA1 in cellular function associated to the regulation of agonist-induced Ca(2+) entry by the modulation of STIM1/Orai1 interaction. ..
  7. ncbi Endogenously generated reactive oxygen species reduce PMCA activity in platelets from patients with non-insulin-dependent diabetes mellitus
    Isaac Jardin
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Platelets 17:283-8. 2006
    ..These findings open up new horizon for the development of therapeutic strategies to palliate cardiovascular disorders in NIDDM...
  8. doi Homers regulate calcium entry and aggregation in human platelets: a role for Homers in the association between STIM1 and Orai1
    Isaac Jardin
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Biochem J 445:29-38. 2012
    ..The findings of the present study support an important role for Homer proteins in thrombin-stimulated platelet function, which is likely to be mediated by the support of agonist-induced Ca²⁺ entry...
  9. doi Store-operated Ca2+ entry is sensitive to the extracellular Ca2+ concentration through plasma membrane STIM1
    Isaac Jardin
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Biochim Biophys Acta 1793:1614-22. 2009
    ..These findings suggest that PM-STIM1 is important for the inactivation of SOCE by extracellular Ca(2+), an event that is likely to be mediated by interaction with Orai1...
  10. doi Orai1 mediates the interaction between STIM1 and hTRPC1 and regulates the mode of activation of hTRPC1-forming Ca2+ channels
    Isaac Jardin
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    J Biol Chem 283:25296-304. 2008
    ..These findings support the functional role of the STIM1-Orai1-hTRPC1 complex in the activation of store-operated Ca(2+) entry...
  11. ncbi TRPC6 participates in the regulation of cytosolic basal calcium concentration in murine resting platelets
    Letizia Albarrán
    Phycell Group, Department of Physiology, University of Extremadura, Caceres, 10003, Extremadura, Spain
    Biochim Biophys Acta 1843:789-96. 2014
    ..Our results suggest an active participation of the intracellular TRPC6 fraction as a regulator of basal [Ca(2+)]c, controlling the passive Ca(2+) leak rate from agonist-sensitive intracellular Ca(2+) stores in resting platelets. ..
  12. doi Lipid rafts are essential for the regulation of SOCE by plasma membrane resident STIM1 in human platelets
    Natalia Dionisio
    Department of Physiology, Cell Physiology Research Group, University of Extremadura, Caceres, Spain
    Biochim Biophys Acta 1813:431-7. 2011
    ..These findings suggest that lipid raft domains are essential for the inactivation of SOCE by extracellular Ca²+ mediated by the interaction between plasma membrane-located STIM1 and Orai1...
  13. ncbi Intracellular Ca2+ homeostasis and aggregation in platelets are impaired by ethanol through the generation of H2O2 and oxidation of sulphydryl groups
    Juan A Rosado
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Arch Biochem Biophys 452:9-16. 2006
    ..In conclusion we provide considerable evidence that ethanol alters Ca2+ entry and reduces thrombin-induced aggregation as a result of the generation of H2O2 and the oxidation of sulphydryl groups in human platelets...
  14. ncbi Hydrogen peroxide generation induces pp60src activation in human platelets: evidence for the involvement of this pathway in store-mediated calcium entry
    Juan A Rosado
    Department of Physiology, Faculty of Veterinary Sciences, University of Extremadura, Caceres 10071, Spain
    J Biol Chem 279:1665-75. 2004
    ....
  15. doi FKBP52 is involved in the regulation of SOCE channels in the human platelets and MEG 01 cells
    Esther Lopez
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Biochim Biophys Acta 1833:652-62. 2013
    ..All together, these results demonstrate that immunophilins participate in the regulation of SOCE in human platelets...
  16. doi Two-pore channel 2 (TPC2) modulates store-operated Ca(2+) entry
    Jose Lopez
    Department of Physiology Cell Physiology Research Group, University of Extremadura, Caceres, Spain
    Biochim Biophys Acta 1823:1976-83. 2012
    ..These results provide strong evidence for modulation of SOCE by TPC2 involving de novo association between TPC2 and STIM1, as well as Orai1, in human cells...
  17. doi STIM1 tyrosine-phosphorylation is required for STIM1-Orai1 association in human platelets
    Esther Lopez
    Cell Physiology Research Group, Department of Physiology, University of Extremadura, 10003 Caceres, Spain
    Cell Signal 24:1315-22. 2012
    ..We finally conclude that STIM1 tyrosine phosphorylation is a key event for the association of STIM1 with plasma membrane Ca(2+) channels such as Orai1, hence it is required for conducting SOCE activation...
  18. ncbi Hydrogen peroxide and peroxynitrite enhance Ca2+ mobilization and aggregation in platelets from type 2 diabetic patients
    Pedro C Redondo
    Department of Physiology, University of Extremadura, Caceres, Spain
    Biochem Biophys Res Commun 333:794-802. 2005
    ..We conclude that H2O2 and ONOO- are likely involved in the enhanced Ca2+ mobilization observed in platelets from type 2 diabetic patients, which might lead to platelet hyperactivity and hyperaggregability...
  19. doi The cytoskeleton plays a modulatory role in the association between STIM1 and the Ca2+ channel subunits Orai1 and TRPC1
    Carmen Galan
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Biochem Pharmacol 82:400-10. 2011
    ..Thus, we demonstrate that the cytoskeleton plays an essential role in the regulation of SOCE through the modulation of the interaction between their main molecular components...
  20. doi Acidic NAADP-releasable Ca(2+) compartments in the megakaryoblastic cell line MEG01
    Natalia Dionisio
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, Caceres, Spain
    Biochim Biophys Acta 1813:1483-94. 2011
    ..TPCs have been shown to be exclusively localized to the endolysosomal system mediating NAADP-evoked Ca(2+) release from the acidic compartments...
  21. doi STIM1 regulates acidic Ca2+ store refilling by interaction with SERCA3 in human platelets
    Jose J Lopez
    Department of Physiology, Cell Physiology Research Group, University of Extremadura, Caceres 10071, Spain
    Biochem Pharmacol 75:2157-64. 2008
    ..This action is impaired in platelets from type 2 diabetics, which might lead to the enhanced cytosolic Ca(2+) concentration observed and, therefore, in platelet hyperactivity...
  22. ncbi Lipid rafts modulate the activation but not the maintenance of store-operated Ca(2+) entry
    Carmen Galan
    Department of Physiology Cell Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Biochim Biophys Acta 1803:1083-93. 2010
    ..In conclusion, lipid raft domains are necessary for the activation but not the maintenance of SOCE probably due to the support of the formation of Ca(2+) signalling complexes involving Orai1, TRPCs and STIM1...
  23. ncbi Cleavage of SNAP-25 and VAMP-2 impairs store-operated Ca2+ entry in mouse pancreatic acinar cells
    Juan A Rosado
    Department of Physiology, University of Extremadura, Caceres, Spain
    Am J Physiol Cell Physiol 288:C214-21. 2005
    ..These findings support a role for SNAP-25 and VAMP-2 in the activation of SOCE in pancreatic acinar cells and show parallels between this process and secretion in a specialized secretory cell type...
  24. ncbi Role of lipid rafts in the interaction between hTRPC1, Orai1 and STIM1
    Isaac Jardin
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, Caceres, Spain
    Channels (Austin) 2:401-3. 2008
    ..These findings suggest that TRPC1, Orai1 and STIM1 form a heteromultimer associated with lipid raft domains and regulated by the intracellular Ca2+ stores...
  25. ncbi Ca2+-independent activation of Bruton's tyrosine kinase is required for store-mediated Ca2+ entry in human platelets
    Pedro C Redondo
    Department of Physiology, University of Extremadura, Spain
    Cell Signal 17:1011-21. 2005
    ..We propose a role for Btk in the early steps of activation of SMCE in human platelets...
  26. ncbi A role for cofilin in the activation of store-operated calcium entry by de novo conformational coupling in human platelets
    Pedro C Redondo
    Department of Physiology, University of Extremadura, Caceres, Spain
    Blood 107:973-9. 2006
    ..These results suggest that cofilin is important for the rapid actin remodeling necessary for the activation of SOCE in platelets through de novo conformational coupling...
  27. doi Functional relevance of the de novo coupling between hTRPC1 and type II IP3 receptor in store-operated Ca2+ entry in human platelets
    Isaac Jardin
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Cell Signal 20:737-47. 2008
    ....
  28. ncbi Two distinct Ca2+ compartments show differential sensitivity to thrombin, ADP and vasopressin in human platelets
    Jose J Lopez
    Department of Physiology, University of Extremadura, Av Universidad s n, Caceres 10071, Spain
    Cell Signal 18:373-81. 2006
    ....
  29. ncbi Melatonin enhances the immune response to vaccination against A1 and C strains of Dichelobacter nodosus
    Sergio Regodón
    Department of Animal Medicine, University of Extremadura, Caceres, Spain
    Vaccine 27:1566-70. 2009
    ..nodosus compared to vaccinated animals not treated with melatonin. Our results suggest that melatonin increased the immune response to vaccination and open new perspectives in the design of prophylactic strategies...
  30. ncbi Interaction of STIM1 with endogenously expressed human canonical TRP1 upon depletion of intracellular Ca2+ stores
    Jose J Lopez
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    J Biol Chem 281:28254-64. 2006
    ..These findings provide evidence for a role of STIM1 in the activation of store-operated Ca(2+) entry probably acting as a Ca(2+) sensor...
  31. pmc A role for SNAP-25 but not VAMPs in store-mediated Ca2+ entry in human platelets
    Pedro C Redondo
    Department of Physiology, University of Extremadura, Caceres, Spain
    J Physiol 558:99-109. 2004
    ..Finally, treatment with BoNT E impaired the coupling between naturally expressed hTRPC1 and IP3 receptor type II in platelets. From these findings we suggest SNAP-25 has a role in SMCE in human platelets...
  32. doi The membrane potential modulates thrombin-stimulated Ca²⁺ mobilization and platelet aggregation
    Letizia Albarrán
    Department of Physiology Cell Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Arch Biochem Biophys 538:130-7. 2013
    ..These observations indicate that PAR-1 and PAR-4 receptors are modulated by the membrane voltage in human platelets. ..
  33. ncbi Involvement of SNARE proteins in thrombin-induced platelet aggregation: evidence for the relevance of Ca2+ entry
    Isaac Jardin
    Department of Physiology, Cellular Physiology Research Group, University of Extremadura, Av Universidad s n, Caceres 10071, Spain
    Arch Biochem Biophys 465:16-25. 2007
    ..These findings support a role for SNAP-25 but not VAMP-1, -2 and -3 in platelet aggregation, which is likely mediated by the regulation of Ca(2+) mobilization in human platelets...
  34. doi Biochemical and functional properties of the store-operated Ca2+ channels
    Gines M Salido
    Department of Physiology Cell Physiology Research Group, University of Extremadura, Caceres 10071, Spain
    Cell Signal 21:457-61. 2009
    ..Interestingly, TRPC1, which has long been proposed as a SOC channel candidate, associates with Orai1 and STIM1 in a ternary complex that appears to increase the variability of SOC currents available to modulate cell function...
  35. ncbi Store-operated Ca2+ entry: vesicle fusion or reversible trafficking and de novo conformational coupling?
    Juan A Rosado
    Department of Physiology, University of Extremadura, Caceres, Spain
    J Cell Physiol 205:262-9. 2005
    ..These are processes in which SNARE proteins might play a crucial role upon cell activation by directing a precise interaction between the membrane of the transported organelle and the PM...
  36. ncbi Dual effect of hydrogen peroxide on store-mediated calcium entry in human platelets
    Pedro C Redondo
    Department of Physiology, Faculty of Veterinary Sciences, University of Extremadura, 10071 Caceres, Spain
    Biochem Pharmacol 67:1065-76. 2004
    ..We conclude that H(2)O(2) exerted a biphasic effect on SMCE. The inhibitory role of high H(2)O(2) concentrations is mediated by an abnormal actin reorganization pattern involving both Ras- and tyrosine kinases-dependent pathways...
  37. doi Unraveling STIM2 function
    Esther Lopez
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, Av Universidad s n, 10003, Caceres, Spain
    J Physiol Biochem 68:619-33. 2012
    ..This review is intended to summarize and provide an overview of the current data available about this exciting isoform, STIM2, and its actual position together with STIM1 in the mechanism of SOCE...
  38. pmc Vaccination prepartum enhances the beneficial effects of melatonin on the immune response and reduces platelet responsiveness in sheep
    Sergio Regodón
    Department of Animal Medicine, University of Extremadura, 10003 Caceres, Spain
    BMC Vet Res 8:84. 2012
    ..Here, we have investigated the role of melatonin as an adjuvant of the Clostridium perfringens vaccine in prepartum sheep and whether melatonin modulates platelet physiology during peripartum...
  39. ncbi Disruption of the filamentous actin cytoskeleton is necessary for the activation of capacitative calcium entry in naive smooth muscle cells
    Sara Morales
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Cell Signal 17:635-45. 2005
    ..In conclusion, these data suggest an active participation of actin reorganization in the implementation of CCE and support a conformational coupling model for this process in naive smooth muscle cells...
  40. ncbi Phosphatidylinositol 4,5-bisphosphate enhances store-operated calcium entry through hTRPC6 channel in human platelets
    Isaac Jardin
    Department of Physiology Cell Physiology Research Group, University of Extremadura, 10071 Caceres, Spain
    Biochim Biophys Acta 1783:84-97. 2008
    ..In summary, hTRPC6 plays a role both in store-operated and in non-capacitative Ca2+ entry. PIP2 enhances store-operated Ca2+ entry in human platelets, most probably by stimulation of hTRPC6 channels...
  41. doi Hepatitis C virus NS5A and core proteins induce oxidative stress-mediated calcium signalling alterations in hepatocytes
    Natalia Dionisio
    Department of Physiology Cell Physiology Research Group, University of Extremadura, Caceres, Spain
    J Hepatol 50:872-82. 2009
    ....
  42. doi Intracellular calcium release from human platelets: different messengers for multiple stores
    Isaac Jardin
    Department of Physiology, Cell Physiology Research Group, University of Extremadura, Caceres 10071, Spain
    Trends Cardiovasc Med 18:57-61. 2008
    ..Here, we review the role of multiple Ca2+ mobilizing messengers and Ca2+ stores in the activation of specific functions in platelets in response to different physiologic agonists...
  43. doi The TRPC ion channels: association with Orai1 and STIM1 proteins and participation in capacitative and non-capacitative calcium entry
    Gines M Salido
    Cell Physiology Group, Department of Physiology, University of Extremadura, Caceres, Spain
    Adv Exp Med Biol 704:413-33. 2011
    ..The present review summarizes the current knowledge concerning the association of TRP proteins with Orai and STIM proteins and how this affects the participation of TRP proteins in store-operated or receptor-operated Ca(2+) entry...
  44. pmc Differential involvement of thrombin receptors in Ca2+ release from two different intracellular stores in human platelets
    Isaac Jardin
    Cell Physiology Research Group, Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Biochem J 401:167-74. 2007
    ..Our results indicate that thrombin-induced activation of PAR-1 and PAR-4 evokes Ca2+ release from both Ca2+ stores, while activation of GPIb-IX-V by thrombin releases Ca2+ solely from the acidic compartments in human platelets...
  45. doi Homer proteins in Ca²⁺ entry
    Isaac Jardin
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, Caceres, Spain
    IUBMB Life 65:497-504. 2013
    ..This review article focuses on the association of Homer 1 proteins with Ca2+-handling proteins and their role on intracellular Ca2+-homeostasis...
  46. pmc Evidence for secretion-like coupling involving pp60src in the activation and maintenance of store-mediated Ca2+ entry in mouse pancreatic acinar cells
    Pedro C Redondo
    Department of Physiology, Faculty of Veterinary Sciences, Av Universidad s n, University of Extremadura, 10071 Caceres, Spain
    Biochem J 370:255-63. 2003
    ..pp60(src) activation requires the integrity of the actin cytoskeleton and participates in the initial phase of the activation of SMCE in pancreatic acinar cells...
  47. pmc Ca2+ accumulation into acidic organelles mediated by Ca2+- and vacuolar H+-ATPases in human platelets
    Jose J Lopez
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Biochem J 390:243-52. 2005
    ..Our results indicate that the TBHQ-sensitive store in human platelets is an acidic organelle whose Ca2+ accumulation is regulated by both Ca2+- and vacuolar H+-ATPases...
  48. doi Store-operated Ca(2+) entry
    Alejandro Berna-Erro
    Department of Physiology, Cell Physiology Research Group, University of Extremadura, Cáceres 10003, Spain
    Adv Exp Med Biol 740:349-82. 2012
    ..The dynamic interaction of STIM1 with Orai1, TRPCs or both might provide an explanation to the distinct capacitative currents described in different cell types...
  49. doi Cinnamtannin B-1 as an antioxidant and platelet aggregation inhibitor
    Jose J Lopez
    Department of Physiology, University of Extremadura, Apdo de Correos 643, 10071, Caceres, Spain
    Life Sci 82:977-82. 2008
    ..This review summarizes the current knowledge on the actions and relevance of the signalling pathways modulated by cinnamtannin B-1...
  50. doi Role of oxidant scavengers in the prevention of Ca²+ homeostasis disorders
    Carmen Galan
    Cell Physiology Research Group, Department of Physiology, University of Extremadura, Caceres 10071, Spain
    Molecules 15:7167-87. 2010
    ..Here, we review the effects of oxidative stress on intracellular Ca²+ homeostasis and the role of antioxidants in the prevention and treatment of disorders associated to abnormal Ca²+ mobilization induced by ROS...
  51. ncbi Alterations in intracellular calcium homeostasis and platelet aggregation induced by ethanol
    Juan A Rosado
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Biochem Biophys Res Commun 341:917-24. 2006
    ..In conclusion, we provide considerable evidence that ethanol reduces thrombin-induced aggregation, which is likely a result of a significant inhibition of Ca(2+) entry, as well as a reduction in the activity of protein tyrosine kinases...
  52. ncbi Pharmacology of TRP channels in the vasculature
    Isaac Jardin
    Department of Physiology, Cell Physiology Research Group, University of Extremadura, 10003, Caceres, Spain
    Curr Vasc Pharmacol 11:480-9. 2013
    ..A number of identified natural compounds and synthetic agents have been reported to modulate TRP function, and are the base for therapeutical strategies. ..
  53. ncbi Molecular interplay between platelets and the vascular wall in thrombosis and hemostasis
    Alejandro Berna-Erro
    Phycell Group, Department of Physiology, University of Extremadura, Caceres, 10003, Extremadura, Spain
    Curr Vasc Pharmacol 11:409-30. 2013
    ..This review focuses on platelet-vascular wall interactions during thrombosis and hemostasis and provides an overview of the main underlying molecular mechanisms. ..
  54. doi The canonical transient receptor potential 6 (TRPC6) channel is sensitive to extracellular pH in mouse platelets
    Alejandro Berna-Erro
    Department of Physiology Cell Physiology Research Group, University of Extremadura, 10003 Caceres, Spain
    Blood Cells Mol Dis 52:108-15. 2014
    ....
  55. pmc Role of Calcium Signals on Hydrogen Peroxide-Induced Apoptosis in Human Myeloid HL-60 Cells
    Ignacio Bejarano
    Department of Physiology, University of Extremadura, Badajoz, Spain
    Int J Biomed Sci 5:246-56. 2009
    ..Our findings suggest that H2O2(-) and thapsigargin-induced apoptosis is dependent on rises in [Ca(2+)]c in human myeloid HL-60 cells...
  56. doi SERCA2b activity is regulated by cyclophilins in human platelets
    Juan A Rosado
    Department of Physiology, University of Extremadura, Avd Universidad s n, Caceres 10071, Spain
    Arterioscler Thromb Vasc Biol 30:419-25. 2010
    ..The role of cyclophilins (chaperones that are widely expressed in different cell types, including human platelets) was explored in sarcoendoplasmic calcium (Ca(2+)) adenosine triphosphatase (SERCA) activity...
  57. doi Melatonin, as an adjuvant-like agent, enhances platelet responsiveness
    Sergio Regodón
    Department of Animal Medicine, University of Extremadura, Caceres, Spain
    J Pineal Res 46:275-85. 2009
    ..These observations suggest that the use of melatonin, as a novel adjuvant, induces beneficial effects on platelet function and haemostasis, and opens new perspectives for therapeutic manipulation of immune responses to vaccination...
  58. ncbi Effect of hydrogen peroxide on Ca2+ mobilisation in human platelets through sulphydryl oxidation dependent and independent mechanisms
    Pedro C Redondo
    Department of Physiology, University of Extremadura, Av Universidad s n, 10071 Caceres, Spain
    Biochem Pharmacol 67:491-502. 2004
    ..In addition, H2O2 induced Ca2+ release from mitochondria and inhibited the PMCA activity by different mechanisms in human platelets...
  59. ncbi Store-operated Ca(2+) entry and tyrosine kinase pp60(src) hyperactivity are modulated by hyperglycemia in platelets from patients with non insulin-dependent diabetes mellitus
    Fernando R Saavedra
    Department of Physiology, University of Extremadura, 10071 Caceres, Spain
    Arch Biochem Biophys 432:261-8. 2004
    ..Both, SOCE and pp60(src) activity in platelets from NIDDM patients are more susceptible to the extracellular glucose concentration, which seems to be involved in the dysfunction of these mechanisms...
  60. ncbi Calcium signalling and reactive oxygen species in non-excitable cells
    Juan A Rosado
    Department of Physiology, University of Extremadura, Caceres, Spain
    Mini Rev Med Chem 6:409-15. 2006
    ..The physiological significance of the role of biological oxidants in the regulation of calcium signalling pathway as well as the mechanisms of the oxidant-stimulation of signal transduction are discussed in this review...
  61. doi TRPC channels and store-operated Ca(2+) entry
    Gines M Salido
    Department of Physiology Cell Physiology Research Group, University of Extremadura, Caceres 10071, Spain
    Biochim Biophys Acta 1793:223-30. 2009
    ..This review attempts to provide an overview of the arguments in favour and against the role of TRPC proteins in the store-operated mechanisms of agonist-activated Ca(2+) entry...
  62. ncbi Melatonin induces mitochondrial-mediated apoptosis in human myeloid HL-60 cells
    Ignacio Bejarano
    Department of Physiology, University of Extremadura, Badajoz, Spain
    J Pineal Res 46:392-400. 2009
    ..We conclude that melatonin has proapoptotic and/or oncostatic effects in the human myeloid cell line HL-60...
  63. ncbi Effects of reactive oxygen species on actin filament polymerisation and amylase secretion in mouse pancreatic acinar cells
    Juan A Rosado
    Department of Physiology, Faculty of Veterinary Sciences, University of Extremadura, Caceres 10071, Spain
    Cell Signal 14:547-56. 2002
    ..The regulation of the actin cytoskeleton by ROS might be involved in radical-induced cell injury in pancreatic acinar cells...
  64. ncbi Discovering the mechanism of capacitative calcium entry
    Juan A Rosado
    Department of Physiology, University of Extremadura, Caceres, Spain
    Am J Physiol Cell Physiol 291:C1104-6. 2006
    ..Effects of MeCh, thapsigargin, and La(3+) on plasmalemmal and intracellular Ca(2+) transport in lacrimal acinar cells. Am J Physiol Cell Physiol 258: C1006-C1015, 1990...
  65. ncbi SERCA2b and 3 play a regulatory role in store-operated calcium entry in human platelets
    Pedro C Redondo
    Department of Physiology, Development and Neuroscience of University of Cambridge, Cambridge, CB3 9ET, United Kingdom
    Cell Signal 20:337-46. 2008
    ..In summary, our results suggest that SERCA2b and 3 modulate thrombin-stimulated SOCE probably by direct interaction with the hTRPC1 channel in human platelets...
  66. doi Intracellular Ca2+ store depletion induces the formation of macromolecular complexes involving hTRPC1, hTRPC6, the type II IP3 receptor and SERCA3 in human platelets
    Pedro C Redondo
    Department of Physiology, Development and Neuroscience, University of Cambridge, CB2 3EG Cambridge, UK
    Biochim Biophys Acta 1783:1163-76. 2008
    ..In conclusion, the present data demonstrate for the first time that hTRPC1, hTRPC6, IP3RII and SERCA3 are parts of a macromolecular protein complex activated by depletion of the intracellular Ca2+ stores in human platelets...
  67. ncbi Dual role of tubulin-cytoskeleton in store-operated calcium entry in human platelets
    Pedro C Redondo
    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, Downing Site, UK
    Cell Signal 19:2147-54. 2007
    ..These findings suggest that tubulin microtubules play a dual role in SOCE, acting as a barrier that prevents constitutive SOCE regulated by DTS, but also supporting SOCE mediated by the acidic Ca(2+) stores...
  68. pmc A role for 5,6-epoxyeicosatrienoic acid in calcium entry by de novo conformational coupling in human platelets
    Nidhal Ben-Amor
    Unité de Recherche de Biochimie, Institute Superieur de Biotechnologie, Monastir, Tunisia
    J Physiol 570:309-23. 2006
    ..We propose that the involvement of 5,6-EET in TG-induced coupling between IP(3)R type II and hTRPC1 and subsequently CCE is compatible with the de novo conformational coupling in human platelets...
  69. ncbi Cinnamtannin B-1 from bay wood reduces abnormal intracellular Ca2+ homeostasis and platelet hyperaggregability in type 2 diabetes mellitus patients
    A Bouaziz
    Unité de Recherche de Biochimie, Institut Superieur de Biotechnologie, 5019 Monastir, Tunisia
    Arch Biochem Biophys 457:235-42. 2007
    ..We conclude that cinnamtannin B-1 exerts an effective antioxidant action in platelets from patients with type 2 diabetes mellitus and reverses the enhanced Ca2+ mobilization and hyperaggregability...
  70. ncbi Thrombin-induced caspases 3 and 9 translocation to the cytoskeleton is independent of changes in cytosolic calcium in human platelets
    Nidhal B Amor
    Unité de Recherche de Biochimie, Institut Superieur de Biotechnologie, 5019 Monastir, Tunisia
    Blood Cells Mol Dis 36:392-401. 2006
    ..These findings suggest that thrombin-induced caspase 9 and 3 activation and translocation are independent on rises in [Ca(2+)](c) but might require store depletion in human platelets...
  71. ncbi Involvement of ryanodine-operated channels in tert-butylhydroperoxide-evoked Ca2+ mobilisation in pancreatic acinar cells
    Maria A Martinez-Burgos
    Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C Ramon y Cajal, 4 18071, Granada, Spain
    J Exp Biol 209:2156-64. 2006
    ..These findings indicate that tBHP releases Ca2+ from non-mitochondrial Ca2+ pools through ryanodine channels...
  72. ncbi Endogenously expressed Trp1 is involved in store-mediated Ca2+ entry by conformational coupling in human platelets
    Juan A Rosado
    Department of Physiology, University of Cambridge, United Kingdom
    J Biol Chem 277:42157-63. 2002
    ..These results provide evidence for the involvement of hTrp1 in the activation of store-mediated Ca(2+) entry by coupling to IP(3)R type II in normal human cells...
  73. ncbi Antiaggregant effects of Arbutus unedo extracts in human platelets
    Mohammed El Haouari
    Laboratoire de Physiologie et Pharmacologie Cellulaire, Departement de Biologie, Université Mohamed 1er, Faculte des Sciences, Bd Mohamed VI, BP 717, Oujda 60000, Morocco
    J Ethnopharmacol 113:325-31. 2007
    ....
  74. ncbi Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets
    Aicha Bouaziz
    Unité de Recherche de Biochimie, Inst Superieur de Biotechnologie, Monastir, Tunisia
    Thromb Haemost 98:375-84. 2007
    ..Both, initial microtubular disassembly and subsequent polymerisation are required for thrombin-induced platelet aggregation and secretion in human platelets...
  75. ncbi Physical properties of two types of calcium stores and SERCAs in human platelets
    Alfonsas Juska
    Vilniaus Gedimino technikos universitetas, Sauletekio al 11, 10223 Vilnius 40, Lithuania
    Mol Cell Biochem 311:9-18. 2008
    ..5 x 10(6 )s(-1) ions per cell. The properties of both calcium stores and the SERCAs and the characteristics found might be related to physiological or pathological state of the cells...
  76. ncbi Dietary virgin olive oil enhances secretagogue-evoked calcium signaling in rat pancreatic acinar cells
    Maria A Martinez
    Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, Granada, Spain
    Nutrition 20:536-41. 2004
    ..Olive oil is a major component of the Mediterranean diet, and its role in human health is actively being debated...
  77. ncbi Characterization of the intracellular mechanisms involved in the antiaggregant properties of cinnamtannin B-1 from bay wood in human platelets
    Nidhal Ben Amor
    Unité de Recherche de Biochimie, Institut Superieur de Biotechnologie, 5019 Monastir, Tunisia
    J Med Chem 50:3937-44. 2007
    ..These observations suggest that cinnamtannin B-1 may prevent thrombotic complications associated to platelet hyperaggregability and hyperactivity, although further studies are necessary to establish appropriate therapeutic strategies...
  78. ncbi Enhanced exocytotic-like insertion of Orai1 into the plasma membrane upon intracellular Ca2+ store depletion
    Geoffrey E Woodard
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Cell Physiol 294:C1323-31. 2008
    ..In aggregate, these findings demonstrate that store depletion enhances Orai1 plasma membrane expression in an exocytotic manner that involves SNAP-25, a process that contributes to store-dependent Ca2+ entry...
  79. ncbi TRP channels and calcium entry in human platelets
    Stewart O Sage
    Blood 100:4245-6; author reply 4246-7. 2002
  80. doi Platelet signalling abnormalities in patients with type 2 diabetes mellitus: a review
    Mohammed El Haouari
    Laboratoire de Physiologie et d Ethnopharmacologie, Departement de Biologie, Université Mohamed 1er, Faculte des Sciences, Bd Mohamed VI, BP 717, Oujda 60000, Morocco
    Blood Cells Mol Dis 41:119-23. 2008
    ..The study of these abnormalities might be helpful in the development of new pharmacological strategies to reduce platelet activation in type 2 diabetes mellitus...
  81. ncbi Caspases 3 and 9 are translocated to the cytoskeleton and activated by thrombin in human platelets. Evidence for the involvement of PKC and the actin filament polymerization
    Nidhal Ben Amor
    Unité de Recherche de Biochimie, Inst Superieur de Biotechnologie, Monastir, Tunisia
    Cell Signal 18:1252-61. 2006
    ....
  82. ncbi Dynamics of calcium fluxes in human platelets assessed in calcium-free medium
    Alfonsas Juska
    Vilniaus Gedimino technikos universitetas, Sauletekio al 11, 10223 Vilnius 40, Lithuania
    Biochem Biophys Res Commun 334:779-86. 2005
    ....
  83. ncbi G-protein coupled receptors and calcium signaling in development
    Geoffrey E Woodard
    Metabolic Diseases Branch, National Institute of Diabetes Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA
    Curr Top Dev Biol 65:189-210. 2005
  84. ncbi A-type natriuretic peptide receptor in the spontaneously hypertensive rat kidney
    Geoffrey E Woodard
    Physiological Laboratory, University of Cambridge, Downing Street, Cambridge, UK
    Peptides 23:1637-47. 2002
    ..The present study provides further evidences for altered NPR-A receptors in SHR kidney, which might act as a negative feedback in response to hypertension...
  85. ncbi Dendroaspis natriuretic peptide-like immunoreactivity and its regulation in rat aortic vascular smooth muscle
    Geoffrey E Woodard
    Department of Physiology, University of Cambridge, Downing Street, CB2 3EG, Cambridge, UK
    Peptides 23:23-9. 2002
    ..Our observations indicate that DNP elicits a marked inhibitory effect on DNA synthesis in culture rat aortic vascular smooth muscle cells...
  86. ncbi Expression and control of C-type natriuretic peptide in rat vascular smooth muscle cells
    Geoffrey E Woodard
    Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, United Kingdom
    Am J Physiol Regul Integr Comp Physiol 282:R156-65. 2002
    ..This provides evidence for a vascular natriuretic peptide autocrine system of physiological relevance in these cells...
  87. ncbi The ERK cascade, a new pathway involved in the activation of store-mediated calcium entry in human platelets
    Juan A Rosado
    Department of Physiology, University of Extremedura, Caceres, Spain
    Trends Cardiovasc Med 12:229-34. 2002
    ..The role of ERK in SMCE into human platelets was found to be independent of the cytoskeleton and a downstream effector of the small guanosine-triphosphate-binding protein Ras...
  88. ncbi Transient receptor potential channels and intracellular signaling
    Geoffrey E Woodard
    Metabolic Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland, USA
    Int Rev Cytol 256:35-67. 2007
    ....