Research Topics
| José E Pérez-OrtínSummaryAffiliation: University of Valencia Country: Spain Publications
| Collaborators
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Detail Information
Publications
Genome-wide studies of mRNA synthesis and degradation in eukaryotesJosé E Pérez-Ortín
Departamento de Bioquimica y Biologia Molecular, Facultad de Biologicas, Universitat de Valencia, C Dr Moliner 50, E46100 Burjassot, Spain
Biochim Biophys Acta 1819:604-15. 2012..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing...
The relative importance of transcription rate, cryptic transcription and mRNA stability on shaping stress responses in yeastJosé García-Martínez
Universitat de Valencia, Valencia, Spain
Transcription 3:39-44. 2012....
Functional analysis of 12 ORFs from Saccharomyces cerevisiae chromosome IIS Rodriguez-Navarro
Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies Biologiques, Universitat de Valencia, Spain
Yeast 15:913-9. 1999..No effects were detected in any of the haploid deletion mutants for the rest of the ORFs with respect to growth, gross morphology or mating...- Genomics and gene transcription kinetics in yeastJosé E Pérez-Ortín
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universitat de Valencia, C Dr Moliner 50, E46100 Burjassot, Spain
Trends Genet 23:250-7. 2007..Such experiments could provide a new picture of the transcriptional response, by enabling us to characterize the kinetic strategies that are used by different genes under given environmental conditions... - DNA chips for yeast biotechnology. The case of wine yeastsJosé E Pérez-Ortín
Departamento de Bioquímica y Biología Molecular and Servicio de Chips de DNA, Universitat de Valencia, Spain
J Biotechnol 98:227-41. 2002..During the past two years some groups have started this study and the first results have been published. We review here the current state of the knowledge of wine yeast and the capacity of DNA chip technology for its improvement... - Molecular characterization of a chromosomal rearrangement involved in the adaptive evolution of yeast strainsJosé E Pérez-Ortín
Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, Spain
Genome Res 12:1533-9. 2002..This is the first time that a gross chromosomal rearrangement is shown to be involved in the adaptive evolution of Saccharomyces cerevisiae... - Genomics of mRNA turnoverJosé E Pérez-Ortín
Departamento de Bioquimica y Biologia Molecular, Facultad de Biologicas, Dr Moliner 50 E46100 Burjassot, Spain
Brief Funct Genomic Proteomic 6:282-91. 2007..They will be described in detail and their potential drawbacks discussed. I will also discuss the integration of the data obtained to fully analyse the expression strategies used by yeast and other eukaryotic cells... - A genomic view of mRNA turnover in yeastJosé E Pérez-Ortín
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universitat de Valencia, Dr Moliner 50, 46100 Burjassot, Spain
C R Biol 334:647-54. 2011.... - Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanismsJosé García-Martínez
Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, Burjassot, Spain
Mol Cell 15:303-13. 2004..These results indicate that, although TR is the main determinant of mRNA abundance in yeast, modulation of mRNA stability is a key factor for gene regulation... - Arginase activity is a useful marker of nitrogen limitation during alcoholic fermentationsPurificación Carrasco
Department de Bioquimica i Biologia Molecular, Facultat de Ciencies Biologiques, Universitat de Valencia, Spain
Syst Appl Microbiol 26:471-9. 2003..The results suggest that ethanol produced during vinification or exogenously added up to 8% (v/v) concentration does not cause nitrogen starvation under the conditions tested because arginase activity is not increased... - Study of the first hours of microvinification by the use of osmotic stress-response genes as probesRoberto Perez-Torrado
Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies Biologiques, Universitat de Valencia, Burjassot, Spain
Syst Appl Microbiol 25:153-61. 2002..These results point out the importance of the control of these parameters and the yeast molecular response during the first hours of vinification for an accurate winemaking process... - Common gene expression strategies revealed by genome-wide analysis in yeastJosé García-Martínez
Sección de Chips de DNA SCSIE, Universitat de Valencia, Dr Moliner 50, E 46100, Burjassot, Spain
Genome Biol 8:R222. 2007..However, because mRNA is an intermediate step and the amounts of both mRNA and protein are controlled by their synthesis and degradation rates, the desired amount of protein can be achieved following different strategies... - Regulon-specific control of transcription elongation across the yeast genomeVicent Pelechano
Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, Burjassot, Spain
PLoS Genet 5:e1000614. 2009..This work demonstrates that the regulation of transcription elongation is a widespread, gene class-dependent phenomenon that also affects housekeeping genes... - The Sas3p and Gcn5p histone acetyltransferases are recruited to similar genesLorena E Rosaleny
Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, Valencia, Spain
Genome Biol 8:R119. 2007..This study is focused on the genome-wide binding of Saccharomyces cerevisiae histone acetyltransferases that are specific for Lys14 of histone H3... - Genomic-wide methods to evaluate transcription rates in yeastJosé García-Martínez
Facultad de Ciencias Biologicas, Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, Burjassot, Spain
Methods Mol Biol 734:25-44. 2011..These techniques allow the discrimination of the relative importance of TR and DR in gene regulation for the first time in a eukaryote... - SRC1: an intron-containing yeast gene involved in sister chromatid segregationSusana Rodriguez-Navarro
Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E 46100 Burjassot, Spain
Yeast 19:43-54. 2002..Deletion of the gene induces a shortening of the anaphase and aggravates the phenotype of scc1 and esp1 conditional mutants, which suggests a direct role of the protein in sister chromatid separation... - Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiaeLorena Romero-Santacreu
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universitat de Valencia, E 46100 Burjassot, Spain
RNA 15:1110-20. 2009..Our results indicate that regulation of mRNA turnover contributes to coordinate gene expression upon osmotic stress, and that there are both specific and global controls of mRNA stability depending on the strength of the osmotic stress... - A complete set of nascent transcription rates for yeast genesVicent Pelechano
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universitat de Valencia, Burjassot, Spain
PLoS ONE 5:e15442. 2010..Histone and ribosomal protein genes are the highest transcribed groups of genes and other than these exceptions the transcription of genes is an infrequent phenomenon in a yeast cell... - The transcriptional inhibitor thiolutin blocks mRNA degradation in yeastVicent Pelechano
Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E46100 Burjassot, Spain
Yeast 25:85-92. 2008..Moreover, the thiolutin-dependent inhibition of mRNA degradation has variable effects on different functional groups of genes, suggesting that they use various degradation pathways for their mRNAs... - There is a steady-state transcriptome in exponentially growing yeast cellsVicent Pelechano
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universitat de Valencia, C Dr Moliner 50, 46100 Burjassot, Spain
Yeast 27:413-22. 2010..With this kinetic approach, we were also able to determine the relative influence of the transcription rate and the mRNA stability changes for the mRNA variation for those genes that deviate from the steady state... - Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6Susana Rodriguez-Navarro
Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E 46100, Burjassot, Spain
Yeast 19:1261-76. 2002..Whereas copy 1 is required for conditions in which B(6) is essential for growth, copies 2 and 3 seem more related with B(1) biosynthesis during the exponential phase... - Nonsense-Mediated mRNA Decay Controls the Changes in Yeast Ribosomal Protein Pre-mRNAs Levels upon Osmotic StressElena Garre
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biológicas Universitat de València, Valencia, Spain
PLoS ONE 8:e61240. 2013..Altogether, our results indicate that the degradation of yeast RP unspliced transcripts by NMD increases during osmotic stress, and suggest that this might be another mechanism to control RP synthesis during the stress response... - Yeast HAT1 and HAT2 deletions have different life-span and transcriptome phenotypesLorena E Rosaleny
, , Dr. Moliner 50, E46100 Burjassot, Spain
FEBS Lett 579:4063-8. 2005..The hat1hat2 double mutant shows a transcriptional phenotype similar to that of the hat1 mutant but strongly enhanced. These results indicate that Hat2p could have additional functions in the cell to those of Hat1p... - mRNAStab--a web application for mRNA stability analysisAndrei Alic
Department of Informatics and Department of Biochemistry and Molecular Biology, Universitat de Valencia, 46100 Burjassot, Spain
Bioinformatics 29:813-4. 2013..It allows the measurement of DR, TR and mRNA levels during cell dynamic responses. Here, we offer a software package that provides improved algorithms for determination of mRNA stability during dynamic GRO experiments... - Genomic insights into the different layers of gene regulation in yeastJosé E Pérez-Ortín
Departamento de Bioquimica y Biologia Molecular, Facultad de Biologicas, Universitat de Valencia, C Dr Moliner 50, 46100 Burjassot, Spain
Genet Res Int 2011:989303. 2011..A comparison between the datasets generated by them provides interesting genomic insights into the different layers of gene regulation in eukaryotes... - A genomic study of the inter-ORF distances in Saccharomyces cerevisiaeVicent Pelechano
, , , C/Dr. Moliner 50, E46100 Burjassot, Spain
Yeast 23:689-99. 2006....